ABSTRACT: Epidemiologic classification of human papillomavirus types associated with cervical ca... more ABSTRACT: Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Engl Epidemiological and research studies have shown that human papillomavirus infections cause cervical cancer. Bosch FX et al, 2002. About 288 000 deaths and 500,000 incidence of cervical cancer are reported worldwide annually. This fact render HPV induced cervical cancer the second most prevalent cancer among women worldwide. To date based on DNA sequence data more than 200 HPV types that demonstrate genomic sequence differences have been recognized. The commonest genotyping methods for HPV are those based on PCR, which employ either general primers followed by genotyping or a serious of type specific PCRs. Even though the clinical validity of HPV genotyping is unquestionable and well documented for that matter; however no HPV genotyping has yet been approved by the US. Food and drug administration (FDA). The development of the current assay involve the combination of the method of multiple sequencing primers with that of DNA pyrosequencing to recognize the 12 most high-risk HPV types namely: HPV-16, HPV-18, HPV-31, HPV-33, HPV-35, HPV-39, HPV-45, HPV-51, HPV-52, HPV-56, HPV-58, and HPV-59. In this study 50 samples extracted from an archival liquid based and formalin fixed paraffin embedded cytology sample of cervical, vulvar and penile cancers were used in this study. HPV DNA samples tested positive for HPV by real-time PCR using GP5+/6+ and the modified MGP5+/6+ were subsequently genotyped by type specific real time PCR and pyrosequencing technology (PSQ) using the multiple sequencing primers (MSP). Moreover efficiency of the conventional GP5+/6+ and the modified MGP5+/6+ primers has been evaluated. Similarly the type specific method of genotyping were challenged by the MSP based pyrosequencing of HPV DNA clinical samples.The sensitivity and validity of genotyping of human papillomaviruses (HPV) by the DNA hybridization techniques like the assay reverse line blot (RLB) and type specific real-time PCR is well documented; however no HPV genotyping has yet been approved by the US. Food and drug administration (FDA).
References: 1. Bosch FX, Lorincz A, Munoz N, Meijer CJ, Shah KV. The causal relationship between human papillomavirus and cervical cancer. J clin pathol. 2002; 55(4): 244-265. 2. Gharizadeh B, Nyrén P, Wallin KL, et al. Sentinel-base DNA genotyping using multiple sequencing primers for high-risk human papillomaviruses. Mol Cell Probes. 2006; 20(3-4): 230-238. 3. Munoz N, Bosch FX, Sanjose S, Herrero R, Castellsague X and Shah KV.
The experience and knowledge I have concerning, Sisay Ambaye MSc, is based on my position, as a c... more The experience and knowledge I have concerning, Sisay Ambaye MSc, is based on my position, as a course director/examine of the Master Degree Projects in Medicine at. Our department is an educational and research department provide educational programs in Medical/Biomedical Sciences including BSc-, MSc-and PhD-levels. Sisay Ambaye has been completed our Master program (Biomedicine and Methods in Medical Diagnostics, profile Experimental Medicine 120 credits), and he was performed well both the theoretical and practical courses. He has completed a 45 ECTS Master Degree Project thesis at the department of Laboratory Medicine, University Hospital Örebro, with a project entitled "Method development and advancement of Human papilloma virus (HPV) detection and genotyping". He carried out his thesis project and passed it very well. Sisay Ambaye is an ambitious student, he showed a great interest in participating in our seminars and research activities, and had a very good social competence and a genuine interest on the research field conducted his Master Thesis. He is an open communicative person and was always followed the instruction's/research's plans etc.. I believe that he has all the qualification and the capacity to complete the Healthcare Leadership and Commissioning course. I warmly recommend him. Sincerely yours, _____________________________________ Nikolaos Venizelos, Professor Senior/Researcher
ABSTRACT: Epidemiologic classification of human papillomavirus types associated with cervical ca... more ABSTRACT: Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Engl Epidemiological and research studies have shown that human papillomavirus infections cause cervical cancer. Bosch FX et al, 2002. About 288 000 deaths and 500,000 incidence of cervical cancer are reported worldwide annually. This fact render HPV induced cervical cancer the second most prevalent cancer among women worldwide. To date based on DNA sequence data more than 200 HPV types that demonstrate genomic sequence differences have been recognized. The commonest genotyping methods for HPV are those based on PCR, which employ either general primers followed by genotyping or a serious of type specific PCRs. Even though the clinical validity of HPV genotyping is unquestionable and well documented for that matter; however no HPV genotyping has yet been approved by the US. Food and drug administration (FDA). The development of the current assay involve the combination of the method of multiple sequencing primers with that of DNA pyrosequencing to recognize the 12 most high-risk HPV types namely: HPV-16, HPV-18, HPV-31, HPV-33, HPV-35, HPV-39, HPV-45, HPV-51, HPV-52, HPV-56, HPV-58, and HPV-59. In this study 50 samples extracted from an archival liquid based and formalin fixed paraffin embedded cytology sample of cervical, vulvar and penile cancers were used in this study. HPV DNA samples tested positive for HPV by real-time PCR using GP5+/6+ and the modified MGP5+/6+ were subsequently genotyped by type specific real time PCR and pyrosequencing technology (PSQ) using the multiple sequencing primers (MSP). Moreover efficiency of the conventional GP5+/6+ and the modified MGP5+/6+ primers has been evaluated. Similarly the type specific method of genotyping were challenged by the MSP based pyrosequencing of HPV DNA clinical samples.The sensitivity and validity of genotyping of human papillomaviruses (HPV) by the DNA hybridization techniques like the assay reverse line blot (RLB) and type specific real-time PCR is well documented; however no HPV genotyping has yet been approved by the US. Food and drug administration (FDA).
References: 1. Bosch FX, Lorincz A, Munoz N, Meijer CJ, Shah KV. The causal relationship between human papillomavirus and cervical cancer. J clin pathol. 2002; 55(4): 244-265. 2. Gharizadeh B, Nyrén P, Wallin KL, et al. Sentinel-base DNA genotyping using multiple sequencing primers for high-risk human papillomaviruses. Mol Cell Probes. 2006; 20(3-4): 230-238. 3. Munoz N, Bosch FX, Sanjose S, Herrero R, Castellsague X and Shah KV.
The experience and knowledge I have concerning, Sisay Ambaye MSc, is based on my position, as a c... more The experience and knowledge I have concerning, Sisay Ambaye MSc, is based on my position, as a course director/examine of the Master Degree Projects in Medicine at. Our department is an educational and research department provide educational programs in Medical/Biomedical Sciences including BSc-, MSc-and PhD-levels. Sisay Ambaye has been completed our Master program (Biomedicine and Methods in Medical Diagnostics, profile Experimental Medicine 120 credits), and he was performed well both the theoretical and practical courses. He has completed a 45 ECTS Master Degree Project thesis at the department of Laboratory Medicine, University Hospital Örebro, with a project entitled "Method development and advancement of Human papilloma virus (HPV) detection and genotyping". He carried out his thesis project and passed it very well. Sisay Ambaye is an ambitious student, he showed a great interest in participating in our seminars and research activities, and had a very good social competence and a genuine interest on the research field conducted his Master Thesis. He is an open communicative person and was always followed the instruction's/research's plans etc.. I believe that he has all the qualification and the capacity to complete the Healthcare Leadership and Commissioning course. I warmly recommend him. Sincerely yours, _____________________________________ Nikolaos Venizelos, Professor Senior/Researcher
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References:
1. Bosch FX, Lorincz A, Munoz N, Meijer CJ, Shah KV. The causal relationship between human papillomavirus and cervical cancer. J clin pathol. 2002; 55(4): 244-265.
2. Gharizadeh B, Nyrén P, Wallin KL, et al. Sentinel-base DNA genotyping using multiple sequencing primers for high-risk human papillomaviruses. Mol Cell Probes. 2006; 20(3-4): 230-238. 3. Munoz N, Bosch FX, Sanjose S, Herrero R, Castellsague X and Shah KV.
References:
1. Bosch FX, Lorincz A, Munoz N, Meijer CJ, Shah KV. The causal relationship between human papillomavirus and cervical cancer. J clin pathol. 2002; 55(4): 244-265.
2. Gharizadeh B, Nyrén P, Wallin KL, et al. Sentinel-base DNA genotyping using multiple sequencing primers for high-risk human papillomaviruses. Mol Cell Probes. 2006; 20(3-4): 230-238. 3. Munoz N, Bosch FX, Sanjose S, Herrero R, Castellsague X and Shah KV.