The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
The protective effects of Sonchus asper methanol extract (SAME) against KBrO 3-induced oxidative ... more The protective effects of Sonchus asper methanol extract (SAME) against KBrO 3-induced oxidative damage in heart tissues of rat were determined in this study. Sprague-Dawley male rats (180 to 200 g) were divided into five groups. Group I: received saline (1 ml/kg b.w., 0.85% NaCl) and DMSO (1 ml/kg b.w.); group II: were injected with KBrO 3 (20 mg/kg b.w., i.p.); group III and IV: treated with KBrO 3 and after 48 with SAME (100; 200 mg/kg b.w.) and group V: with SAME (200 mg/kg b.w.). All the treatments were given twice a week for four weeks. The results revealed that KBrO 3 induced oxidative stress as evidenced by the significant decrease in the activity levels of antioxidant enzymes (CAT, POD, SOD, GSH-Px, GSR, GST, QR, XO) and glutathione (GSH), while increase in the levels of γ-GT, MDA and H 2 O 2 in cardiac samples compared with the control group. Serum levels of CK, CK-MB, LDH, uric acid, AST, ALT, ALP, triglycerides, total cholesterol, LDL, VLDL were elevated while HDL level was significantly reduced in KBrO 3 group compared with the control. Since SAME treatment reversed these responses dose dependently, it seems likely that S. asper methanol extract can protect the cardiac tissues against KBrO 3 mediated oxidative damage.
The protective effects of Sonchus asper methanol extract (SAME) against KBrO 3-induced oxidative ... more The protective effects of Sonchus asper methanol extract (SAME) against KBrO 3-induced oxidative damage in heart tissues of rat were determined in this study. Sprague-Dawley male rats (180 to 200 g) were divided into five groups. Group I: received saline (1 ml/kg b.w., 0.85% NaCl) and DMSO (1 ml/kg b.w.); group II: were injected with KBrO 3 (20 mg/kg b.w., i.p.); group III and IV: treated with KBrO 3 and after 48 with SAME (100; 200 mg/kg b.w.) and group V: with SAME (200 mg/kg b.w.). All the treatments were given twice a week for four weeks. The results revealed that KBrO 3 induced oxidative stress as evidenced by the significant decrease in the activity levels of antioxidant enzymes (CAT, POD, SOD, GSH-Px, GSR, GST, QR, XO) and glutathione (GSH), while increase in the levels of γ-GT, MDA and H 2 O 2 in cardiac samples compared with the control group. Serum levels of CK, CK-MB, LDH, uric acid, AST, ALT, ALP, triglycerides, total cholesterol, LDL, VLDL were elevated while HDL level was significantly reduced in KBrO 3 group compared with the control. Since SAME treatment reversed these responses dose dependently, it seems likely that S. asper methanol extract can protect the cardiac tissues against KBrO 3 mediated oxidative damage.
This study examined the sex differences for physical, morphological, histological, mRNA, and prot... more This study examined the sex differences for physical, morphological, histological, mRNA, and protein expression levels changes for interleukins and natriuretic peptides in left ventricle (LV) of two groups of adult FVB/N mice; males (WM) and females (WF). LV morphological, histological, reverse transcription and quantitative real-time PCR (RT-PCR), and immunohistochemical (IHC) alterations were determined in FVB/N mice at 34-35 weeks on gender basis. Confirming the gender dimorphism, FVB/N males (WM) illustrated a significant reduction in ANP and IL1-A levels as well as significantly increased body weight (BW (gm)), tibia length (TL (mm)), heart weight (HW (mg)), heart weight-to-body weight (HW/BW (mg/gm)) ratio, heart weight-to-tibia length (HW/TL (mg/mm)) ratio, left ventricle weight (LV (mg)), left ventricle-to-body weight (LV/BW (mg/gm)) ratio, and left ventricle-to-tibia length (LV/TL (mg/mm)) ratio, left ventricular (LV) cardiomyocyte diameter, high BNP, NPRA, IL-1B, and IL1R1 expression in comparison with FVB/N females (WF). Gender differences in relation to left ventricle (LV) may be due to differences in the interleukins and natriuretic peptides levels as an outcome of sex related hormones.
To investigate the potential role of IL-1A gene variations in pathogenesis of coronary artery dis... more To investigate the potential role of IL-1A gene variations in pathogenesis of coronary artery disease (CAD) with familial history. We investigated the IL-1A-889C>T polymorphism in 335 patients with CAD and 335 healthy individuals for case-control association analysis. In this study, we also investigated the heritability of the susceptible allele from 130 trio families with CAD-affected offspring. We performed genotyping using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The IL-1A-889 C/T polymorphism was significantly associated with CAD in patients compared with healthy controls. The minor allele T at -889 was more prevalent in cases vs controls. The results of a transmission disequilibrium test revealed a significant association between the IL-1A-889 polymorphism and CAD. The mutant genotype CT+TT was significantly associated with high levels of high-sensitivity C-reactive protein (CRP) and other relative markers from patients with C...
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately.
The protective effects of Sonchus asper methanol extract (SAME) against KBrO 3-induced oxidative ... more The protective effects of Sonchus asper methanol extract (SAME) against KBrO 3-induced oxidative damage in heart tissues of rat were determined in this study. Sprague-Dawley male rats (180 to 200 g) were divided into five groups. Group I: received saline (1 ml/kg b.w., 0.85% NaCl) and DMSO (1 ml/kg b.w.); group II: were injected with KBrO 3 (20 mg/kg b.w., i.p.); group III and IV: treated with KBrO 3 and after 48 with SAME (100; 200 mg/kg b.w.) and group V: with SAME (200 mg/kg b.w.). All the treatments were given twice a week for four weeks. The results revealed that KBrO 3 induced oxidative stress as evidenced by the significant decrease in the activity levels of antioxidant enzymes (CAT, POD, SOD, GSH-Px, GSR, GST, QR, XO) and glutathione (GSH), while increase in the levels of γ-GT, MDA and H 2 O 2 in cardiac samples compared with the control group. Serum levels of CK, CK-MB, LDH, uric acid, AST, ALT, ALP, triglycerides, total cholesterol, LDL, VLDL were elevated while HDL level was significantly reduced in KBrO 3 group compared with the control. Since SAME treatment reversed these responses dose dependently, it seems likely that S. asper methanol extract can protect the cardiac tissues against KBrO 3 mediated oxidative damage.
The protective effects of Sonchus asper methanol extract (SAME) against KBrO 3-induced oxidative ... more The protective effects of Sonchus asper methanol extract (SAME) against KBrO 3-induced oxidative damage in heart tissues of rat were determined in this study. Sprague-Dawley male rats (180 to 200 g) were divided into five groups. Group I: received saline (1 ml/kg b.w., 0.85% NaCl) and DMSO (1 ml/kg b.w.); group II: were injected with KBrO 3 (20 mg/kg b.w., i.p.); group III and IV: treated with KBrO 3 and after 48 with SAME (100; 200 mg/kg b.w.) and group V: with SAME (200 mg/kg b.w.). All the treatments were given twice a week for four weeks. The results revealed that KBrO 3 induced oxidative stress as evidenced by the significant decrease in the activity levels of antioxidant enzymes (CAT, POD, SOD, GSH-Px, GSR, GST, QR, XO) and glutathione (GSH), while increase in the levels of γ-GT, MDA and H 2 O 2 in cardiac samples compared with the control group. Serum levels of CK, CK-MB, LDH, uric acid, AST, ALT, ALP, triglycerides, total cholesterol, LDL, VLDL were elevated while HDL level was significantly reduced in KBrO 3 group compared with the control. Since SAME treatment reversed these responses dose dependently, it seems likely that S. asper methanol extract can protect the cardiac tissues against KBrO 3 mediated oxidative damage.
This study examined the sex differences for physical, morphological, histological, mRNA, and prot... more This study examined the sex differences for physical, morphological, histological, mRNA, and protein expression levels changes for interleukins and natriuretic peptides in left ventricle (LV) of two groups of adult FVB/N mice; males (WM) and females (WF). LV morphological, histological, reverse transcription and quantitative real-time PCR (RT-PCR), and immunohistochemical (IHC) alterations were determined in FVB/N mice at 34-35 weeks on gender basis. Confirming the gender dimorphism, FVB/N males (WM) illustrated a significant reduction in ANP and IL1-A levels as well as significantly increased body weight (BW (gm)), tibia length (TL (mm)), heart weight (HW (mg)), heart weight-to-body weight (HW/BW (mg/gm)) ratio, heart weight-to-tibia length (HW/TL (mg/mm)) ratio, left ventricle weight (LV (mg)), left ventricle-to-body weight (LV/BW (mg/gm)) ratio, and left ventricle-to-tibia length (LV/TL (mg/mm)) ratio, left ventricular (LV) cardiomyocyte diameter, high BNP, NPRA, IL-1B, and IL1R1 expression in comparison with FVB/N females (WF). Gender differences in relation to left ventricle (LV) may be due to differences in the interleukins and natriuretic peptides levels as an outcome of sex related hormones.
To investigate the potential role of IL-1A gene variations in pathogenesis of coronary artery dis... more To investigate the potential role of IL-1A gene variations in pathogenesis of coronary artery disease (CAD) with familial history. We investigated the IL-1A-889C>T polymorphism in 335 patients with CAD and 335 healthy individuals for case-control association analysis. In this study, we also investigated the heritability of the susceptible allele from 130 trio families with CAD-affected offspring. We performed genotyping using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The IL-1A-889 C/T polymorphism was significantly associated with CAD in patients compared with healthy controls. The minor allele T at -889 was more prevalent in cases vs controls. The results of a transmission disequilibrium test revealed a significant association between the IL-1A-889 polymorphism and CAD. The mutant genotype CT+TT was significantly associated with high levels of high-sensitivity C-reactive protein (CRP) and other relative markers from patients with C...
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