Peste-des-petits ruminants, is an important viral contagious disease of sheep and goat. Consideri... more Peste-des-petits ruminants, is an important viral contagious disease of sheep and goat. Considering the importance of PPR in Sistan region, its economic losses as a consequence of mortality and treatment expenses, the high consumption of animal meat in the traditional way of slaughter in this area and lack of adequate supervision on animal health, the present study was conducted with the aim of histopathological investigation of this disease in sheep and goats of Sistan region. Tissue specimens from mouth, lungs, intestines and lymph nodes of 30 cases from suspected flocks were collected and after tissue processing were microscopically examined. In microscopic investigation, lesions were observed in 9 specimens. The data were analyzed using SPSS software and Kappa test. The incidence of infection in sheep and goats was 33.25% and 38/88% respectively and significant association was observed between species and lesions such as multinucleated inflammatory cells accumulation and syncyti...
BACKGROUND: Toxoplasmosis is one of the most prevalent parasitic diseases in human societies and ... more BACKGROUND: Toxoplasmosis is one of the most prevalent parasitic diseases in human societies and ani- mal populations, particularly pregnant women and domestic animals. This life-threatening infection may cause severe consequences in the fetus. OBJECTIVES: This study was aimed to estimate the prevalence of anti-parasite antibodies in pregnant wom- en and sheep and goat populations of Zabol city, Sistan and Baluchistan Province, Southeast Iran. METHODS: Ninety serum samples of pregnant women and 184 serum samples of sheep and goats were collected and anti-Toxoplasma IgG and IgM antibodies were examined using a commercial enzyme-linked immunosorbent assay kit. Subsequently, the correlation between the seroprevalence of infection and socio-de- mographic data was statistically calculated. RESULTS: Among pregnant women, 13/90 (14%) samples were IgG positive and seroprevalence was signifi- cantly correlated to history of abortion (p <0.05). Among examined sheep and goats, 34 sheep (24....
In recent years, popularity of horse-riding has increased which has caused many consequences and ... more In recent years, popularity of horse-riding has increased which has caused many consequences and problems. One of the important issues raised in equestrian sports is the abuse of different drugs for doping. It has been reported that flunixin meglumine has been mainly used for doing of horses in recent years in Iran and also most of doping cases are of morphine type. In this study, urinary morphine detection kit, with the ability to detect at least 300 mg/ml morphine in urine, manufactured by a France company named ABON® was used for early detection of morphine in horse doping. For this purpose, 36 horses of both sexes (26 horses in the experimental group and 10 horses as the control) were obtained. Horses in the experimental group were intramuscularly treated with 0.4 mg/kg morphine sulfate produced by Daroo Pakhsh Company. In addition, 10 control horses received distilled water by the same amount. Then, urine samples were collected 3, 24, and 48 hours after administration and teste...
The equine herpesviruses (EHVs) are important pathogens in all members of Equidae family world wi... more The equine herpesviruses (EHVs) are important pathogens in all members of Equidae family world wide. Because of the causative agents of several diseases and latency makes major challenges to equine industry. In the present study blood samples of 108 mules and donkeys from different rural areas of North-East of Iran, as a region with most equine population, were examined for presence of Equine herpesvirus -1 and 4 using real time PCR as a rapid molecular diagnostic test that has high sensitivity and specificity. Based on results, it was conclude that EHV-1 DNA was not detected in any blood samples whereas EHV-4 was present in 14.8% of donkeys and mules. Which can have important role as a source of infection for other equids population.
Theileria, Babesia, and Anaplasma are tick-borne pathogens affecting livestock industries worldwi... more Theileria, Babesia, and Anaplasma are tick-borne pathogens affecting livestock industries worldwide. In this study, we surveyed the presence of Babesia ovis, Theileria ovis, Theileria lestoquardi, Anaplasma ovis,Anaplasma phagocytophilum, and Anaplasma marginale in 200 goats from 3 different districts in Sistan and Baluchestan province, Iran. Species-specific diagnostic PCR and sequence analysis revealed that 1.5%, 12.5%, and 80% of samples were positive for T. lestoquardi, T. ovis, and A. ovis, respectively. Co-infections of goats with up to 3 pathogens were seen in 22% of the samples. We observed a positive correlation between A. ovis and T. ovis infection. In addition, by analyzing the data with respect to Plasmodium caprae infection in these goats, a negative correlation was found between P. caprae and A. ovis and between P. caprae and T. ovis. This study contributes to understanding the epidemiology of vector-borne pathogens and their interplay in goats.
A correction to this article has been published and is linked from the HTML and PDF versions of t... more A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
Plasmodium was first identified in a goat in Angola in 1923, and only recently characterized by D... more Plasmodium was first identified in a goat in Angola in 1923, and only recently characterized by DNA isolation from a goat blood sample in Zambia. Goats were first domesticated in the Fertile Crescent approximately 10,000 years ago, and are now globally distributed. It is not known if the Plasmodium identified in African goats originated from parasites circulating in the local ungulates, or if it co-evolved in the goat before its domestication. To address this question, we performed PCR-based surveillance using a total of 1,299 goat blood samples collected from Sudan and Kenya in Africa, Iran in west Asia, and Myanmar and Thailand in southeast Asia. Plasmodium DNA was detected from all locations, suggesting that the parasite is not limited to Africa, but widely distributed. Whole mitochondrial DNA sequences revealed that there was only one nucleotide substitution between Zambian/Kenyan samples and others, supporting the existence of a goat-specific Plasmodium species, presumably Plas...
Peste-des-petits ruminants, is an important viral contagious disease of sheep and goat. Consideri... more Peste-des-petits ruminants, is an important viral contagious disease of sheep and goat. Considering the importance of PPR in Sistan region, its economic losses as a consequence of mortality and treatment expenses, the high consumption of animal meat in the traditional way of slaughter in this area and lack of adequate supervision on animal health, the present study was conducted with the aim of histopathological investigation of this disease in sheep and goats of Sistan region. Tissue specimens from mouth, lungs, intestines and lymph nodes of 30 cases from suspected flocks were collected and after tissue processing were microscopically examined. In microscopic investigation, lesions were observed in 9 specimens. The data were analyzed using SPSS software and Kappa test. The incidence of infection in sheep and goats was 33.25% and 38/88% respectively and significant association was observed between species and lesions such as multinucleated inflammatory cells accumulation and syncyti...
BACKGROUND: Toxoplasmosis is one of the most prevalent parasitic diseases in human societies and ... more BACKGROUND: Toxoplasmosis is one of the most prevalent parasitic diseases in human societies and ani- mal populations, particularly pregnant women and domestic animals. This life-threatening infection may cause severe consequences in the fetus. OBJECTIVES: This study was aimed to estimate the prevalence of anti-parasite antibodies in pregnant wom- en and sheep and goat populations of Zabol city, Sistan and Baluchistan Province, Southeast Iran. METHODS: Ninety serum samples of pregnant women and 184 serum samples of sheep and goats were collected and anti-Toxoplasma IgG and IgM antibodies were examined using a commercial enzyme-linked immunosorbent assay kit. Subsequently, the correlation between the seroprevalence of infection and socio-de- mographic data was statistically calculated. RESULTS: Among pregnant women, 13/90 (14%) samples were IgG positive and seroprevalence was signifi- cantly correlated to history of abortion (p <0.05). Among examined sheep and goats, 34 sheep (24....
In recent years, popularity of horse-riding has increased which has caused many consequences and ... more In recent years, popularity of horse-riding has increased which has caused many consequences and problems. One of the important issues raised in equestrian sports is the abuse of different drugs for doping. It has been reported that flunixin meglumine has been mainly used for doing of horses in recent years in Iran and also most of doping cases are of morphine type. In this study, urinary morphine detection kit, with the ability to detect at least 300 mg/ml morphine in urine, manufactured by a France company named ABON® was used for early detection of morphine in horse doping. For this purpose, 36 horses of both sexes (26 horses in the experimental group and 10 horses as the control) were obtained. Horses in the experimental group were intramuscularly treated with 0.4 mg/kg morphine sulfate produced by Daroo Pakhsh Company. In addition, 10 control horses received distilled water by the same amount. Then, urine samples were collected 3, 24, and 48 hours after administration and teste...
The equine herpesviruses (EHVs) are important pathogens in all members of Equidae family world wi... more The equine herpesviruses (EHVs) are important pathogens in all members of Equidae family world wide. Because of the causative agents of several diseases and latency makes major challenges to equine industry. In the present study blood samples of 108 mules and donkeys from different rural areas of North-East of Iran, as a region with most equine population, were examined for presence of Equine herpesvirus -1 and 4 using real time PCR as a rapid molecular diagnostic test that has high sensitivity and specificity. Based on results, it was conclude that EHV-1 DNA was not detected in any blood samples whereas EHV-4 was present in 14.8% of donkeys and mules. Which can have important role as a source of infection for other equids population.
Theileria, Babesia, and Anaplasma are tick-borne pathogens affecting livestock industries worldwi... more Theileria, Babesia, and Anaplasma are tick-borne pathogens affecting livestock industries worldwide. In this study, we surveyed the presence of Babesia ovis, Theileria ovis, Theileria lestoquardi, Anaplasma ovis,Anaplasma phagocytophilum, and Anaplasma marginale in 200 goats from 3 different districts in Sistan and Baluchestan province, Iran. Species-specific diagnostic PCR and sequence analysis revealed that 1.5%, 12.5%, and 80% of samples were positive for T. lestoquardi, T. ovis, and A. ovis, respectively. Co-infections of goats with up to 3 pathogens were seen in 22% of the samples. We observed a positive correlation between A. ovis and T. ovis infection. In addition, by analyzing the data with respect to Plasmodium caprae infection in these goats, a negative correlation was found between P. caprae and A. ovis and between P. caprae and T. ovis. This study contributes to understanding the epidemiology of vector-borne pathogens and their interplay in goats.
A correction to this article has been published and is linked from the HTML and PDF versions of t... more A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
Plasmodium was first identified in a goat in Angola in 1923, and only recently characterized by D... more Plasmodium was first identified in a goat in Angola in 1923, and only recently characterized by DNA isolation from a goat blood sample in Zambia. Goats were first domesticated in the Fertile Crescent approximately 10,000 years ago, and are now globally distributed. It is not known if the Plasmodium identified in African goats originated from parasites circulating in the local ungulates, or if it co-evolved in the goat before its domestication. To address this question, we performed PCR-based surveillance using a total of 1,299 goat blood samples collected from Sudan and Kenya in Africa, Iran in west Asia, and Myanmar and Thailand in southeast Asia. Plasmodium DNA was detected from all locations, suggesting that the parasite is not limited to Africa, but widely distributed. Whole mitochondrial DNA sequences revealed that there was only one nucleotide substitution between Zambian/Kenyan samples and others, supporting the existence of a goat-specific Plasmodium species, presumably Plas...
Uploads