shivani R pandya

Magnetic nanoparticle accumulated dendrimers (MADs) have been prepared using a 1 : 1 ratio of MNPs and trimesoyl 1,3,5-trimethyl malonate ester (TTDMM), a 1st tier dendrimer, in ethanolic medium at 100 °C on refluxing for 24 h. The MADs formed were characterized for their structural, functional and morphological properties using XRD, VSM, FTIR, SEM-EDX, TEM and DLS techniques. The MNPs show superparamagnetic character which moderates the structural ability of TTDMM to bind silibinin (SB) and methotrexate (MTX) anticancer drugs for their potential use in drug delivery systems. In this context, the MADs have shown higher silibinin and methotrexate binding activities compared to those of the dendrimer only, which are confirmed through FTIR, DLS and SEM-EDX. An UV-Vis spectrophotometry study shows 32.65% and 53.03% in vitro release of SB and MTX respectively at 10 h, in PBS with a 10% DMSO (PD) medium at 37 °C. Furthermore, chemosensitivity testing of MAD was carried out on a human lung...

Abstract Nowadays smart and multifunctional materials with unique optical and several other physicochemical properties have pooled up potential to contribute with various applications in science and technological development. We have initiated a new innovative research work for synthesis of lanthanide nanocomposite by developing certain stoichiometric functional combinations of proteins and lanthanides as per energy distribution and Boltzmann partition functions which are based on capping, binding or occupying surface areas by adsorption on generating suitable Vander Waals and London dispersive forces as functional materials matrix. This work aims to synthesize a novel Albumen-CeO2 Biofunctional nanocomposite (BFNC) and further study its potential antioxidant properties. In our work, 25µM to 150 µM CeO2NP with 150µM Albumen Physicochemical interaction was carried out to understand the optical behavior and aggregation. The result shows maximum interaction with 1:1 150µM CeO2NP: Albumen confirmed through UV-Vis spectrophotometer. The CeO2-Albumen BFNC was synthesized by facile eco-friendly ex-situ methodology with hydrodynamic diameter of 110.6 nm confirmed by Dynamic light scattering (DLS). The BFNC were characterized by UV-Vis spectrophotometer, FT-IR and DLS. The bare CeO2 NP and synthesized BFNC were tested for Antioxidant property by using 2, 2-Diphenyl-1-picrylhydrazyl (DPPH•). Thus BFNC shows 48.92% scavenging activity compared to that of 22.71% scavenging activity of bare CeO2NP. Thus the present research work shows that the design of such lanthanide based BFNC which exhibit unusual optical and surface functionalized biological properties could induce suitable biomedical properties for the host matrix, which can be used for the development of new inorganic or organic hybrid matrix systems.

Abstract Biofunctionalized magnetic nanoparticles (FMNPs) were synthesized through in situ coprecipitations of Fe (II) and Fe (III) salts under air followed by the addition of amino acids (AA): serine (Ser), alanine (Ala), cysteine (Cys), histidine (His), and methionine (Met) via bottom-up approach without following any sophisticated route. However, the effect of amino acid side chain on the formation of γ-Fe2O3 (maghemite) and Fe3O4 (magnetite) was attained through XRD, HR-TEM, SEM, VSM, FTIR, DLS and Mercury Porosimeter (MP). Surprisingly, our study depicts that except His; Ser, Ala, Cys and Met produced more than 50% of magnetite phase and inhibited the oxidation of Fe3O4 in the presence of air. Furthermore, AA@MNPs were used for in vitro biological assessment such as salmon testes DNA (ST-DNA) binding efficiency, DPPH scavenging activity, antimicrobial and anticancer properties using UV–visible spectrophotometry and colorimeter respectively. The ST-DNA binding efficiency was observed at λmax 259 nm and quantitative interaction was determined using 15–125 µM AA@MNPs and 500 µg/ml DNA at physiological temperature, shows stacking interaction. The Cys@MNPs have exhibited highest DPPH scavenging activity with SC50 2.5 µg/mL at λmax 525 nm. Antimicrobial property of AA@MNPs was studied on gram-positive (B. subtilis), gram-negative (E. coli) bacteria and fungi (C. albicans) using disc diffusion assay by measuring zone of inhibition (ZOI) in mm, mentioned as a diameter. Alongside, minimum inhibition concentration (MIC) was estimated by the dilution method for those strains sensitive against AA@MNPs. Although, AA@MNPs showed ∼100% control growth of human lung cancer cell line (A549) with LC50, TGI and GI50 >80 µg/ml shown cells compatibility. These significant results revealed the impact of AA side chain with different surface activities of FMNPs.

Synthesis of Magnetic nanoparticles were made using coprecipitation method on mixing Fe+3 and Fe+2 in 2:1 ratio with aqueous 8M NaOH which on heating at 90°C for 2 h has yielded 85% magnetic (Fe3O4) nanoparticles (MNPs), characterized by XRD, VSM, SEM, and HR-TEM. The formic acid (FA), oxalic acid (OA) and citric acid (CA), the series of aliphatic organic acids along with Trimesoyl 1, 3, 5 tridimethyl malonate (TTDMM), trimesoyl 1, 3, 5 tridiethyl malonate (TTDEM), trimesoyl 1, 3, 5 tridipropyl malonate (TTDPM), trimesoyl 1, 3, 5 tridibutyl malonate (TTDBM) and trimesoyl 1, 3, 5 tridihexyl malonate (TTDHM) 1st tier dendrimers were used separately for preparing nanomagnetic fluid. From 25 to 150 µM MNPs at an interval of 25 µM were dispersed in 150 µM of acids and dendrimers separately with DMSO. UV-VIS spectrophotometry showed a maximum MNPs dispersion with TTDMM against others and found to be most stable nanomagnetic fluid on account of capping type mechanism of acids.