Papers by Mark David De Guzman
Phylogenetic Analysis and DNA Barcoding of Hedychium philippinense K. Schum., 2018
Hedychium philippinense is an endemic flowering plant from the Zingiberaceae family and is one of... more Hedychium philippinense is an endemic flowering plant from the Zingiberaceae family and is one of the species under the genus Hedychium. Since this plant is endemic to the Philippines and is endangered the number of related studies regarding to H. philippinense is insufficient or low. Here researchers present results of a phylogenetic analysis of 10 species including H. philippinense. The plant material was collected in its matured stage from the Laguna-Quezon Land Grant. The ITS2 was the chosen gene since accession numbers of the ITS2 sequences from Hedychium sequence produced fully resolved trees in other species of Zingiberaceae. There were 2 major groups generated using likelihood maximum method in Mega7, the first group has a bootstrap of 41 and the second group with a bootstrap of 81. The molecular phylogenetic analysis created for tha analysation of H. philippinense shows that it formed a sister clade in H. coronarium furthermore of selected Hedychium species it also shows that there are two outgroup species namely the Zingiber zerumbet and Boesenbergia sp. The average interspecific variation calculated was 0.024443 which is low in general. The ITS2 is not that varying among Hedychium species but by the addition of other taxa, the inter specific value rose to 0.09. ITS2 have a significant inter and intraspecific variations it also showed the highest inter and intraspecific distances between species of Zingiberaceae as compared to other loci. The DNA sequence that was obtained using ITS primers were different from those found on Nucleotide/DNA sequences available on different databases. Results indicate that the phylogenetic placement of Hedychium species may help to broaden the understanding of the ITS2 capability.
Thesis Chapters by Mark David De Guzman
Floral Inventory of Polytechnic University of the Philippines - Mulanay Campus, Quezon Province, Apr 16, 2019
The Polytechnic University of the Philippines - Mulanay Campus is the largest of the three PUP ca... more The Polytechnic University of the Philippines - Mulanay Campus is the largest of the three PUP campuses in Quezon Province, its strategic location near the shore of Tayabas Bay which possess high levels of species endemism and an abundance of forest vegetation. Floral inventory was conducted in the campus to determine the species diversity of fern, fern allies, gymnosperms and angiosperms. Opportunistic sampling was used as a method in collection of vascular plant specimens. A total of 123 plant species belonging to 75 genera and 50 families in PUP Mulanay. Among of the 50 families collected in PUP Mulanay, 6 families are Pteridophytes and 1 family of gymnosperm were recorded. The remaining 43 families were angiosperm, among these the family Poaceae dominates in the study area.
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Papers by Mark David De Guzman
Thesis Chapters by Mark David De Guzman