Papers by Luciano Brambilla
FEMS microbiology letters, 2014
The OmpW family consists of a ubiquitous group of small outer membrane (OM) β-barrel proteins of ... more The OmpW family consists of a ubiquitous group of small outer membrane (OM) β-barrel proteins of Gram-negative bacteria with proposed roles in environmental adaptation but poorly understood mechanisms of expression. We report here that E. coli K-12 OmpW contents are drastically modified by temperature changes compatible with the leap from the environment to warm-blooded hosts and/or vice versa. Thus, while OmpW is present in the OM of bacteria grown at 37°C, it sharply disappears at 23°C with the concomitant acquisition of colicin S4 resistance by the cells. ompW::lacZY fusions indicated that temperature regulation operates at the level of transcription, being ompW expression almost abolished at 23ºC as compared to 37°C. Moreover, E. coli Δhns mutants lacking H-NS showed reductions in ompW transcription and OmpW contents at 37ºC indicating positive modulatory roles for this nucleoid-structuring protein in ompW expression. Also, ΔhnsΔstpA double mutants simultaneously lacking H-NS and its paralog StpA showed more severe reductions in ompW expression at 37ºC resulting in the complete loss of OmpW. The overall results indicate that OmpW contents in E. coli are regulated by both temperature and H-NS, and reinforce OmpW functions in bacterial adaptation to warm blooded hosts.This article is protected by copyright. All rights reserved.
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Antimicrobial agents and chemotherapy, 2014
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Antimicrobial agents and chemotherapy, 2013
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Journal of Plant Growth Regulation, 2006
The synthesis of isochroman derivatives 4–9 from α-hydroxylactone 3 is reported. These heterocyc... more The synthesis of isochroman derivatives 4–9 from α-hydroxylactone 3 is reported. These heterocycles, carrying different substituents on C-3, C-4, and C-8, exhibited different degrees of inhibition of the vegetative growth of wheat (Triticum aestivum cv Klein Escorpion) plants, whereas plant developmental patterns such as their protein profile, carotenes/chlorophylls ratio, and weight/length relationship were not significantly affected. Microscopic observation of transverse sections of shoots and roots showed morphological changes in the treated plants, consistent with delayed development. The results suggest that among these isochromans the C-3 carbonyl moiety of the lactone and the C-4 free hydroxyl group are important but not essential for activity, and that a short side chain appended to C-3 is tolerated. However, cleavage of the C-8 methyl ether group to the related free phenol causes a drastic reduction in the growth inhibitory activity.
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Capítulo: "Genealogía de la familia Arnold"
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Capítulo: "Autoridades, Alcaldes de la Santa Hermandad, comisarios y jueces de paz en el actual d... more Capítulo: "Autoridades, Alcaldes de la Santa Hermandad, comisarios y jueces de paz en el actual distrito Álvarez (Santa Fe)"
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Historia by Luciano Brambilla
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Papers by Luciano Brambilla
Historia by Luciano Brambilla