Vanessa K Hilliard Young

Members of several terrestrial vertebrate lineages have returned to nearly exclusive use of aquatic habitats. These transitions were often accompanied by changes in skeletal morphology, such as flattening of limb bone shafts. Such morphological changes might be correlated with the exposure of limb bones to altered loading. Though the environmental forces acting on the skeleton differ substantially between water and land, no empirical data exist to quantify the impact of such differences on the skeleton, either in terms of load magnitude or regime. To test how locomotor loads change between water and land, we compared in vivo strains from femora of turtles (Trachemys scripta) during swimming and terrestrial walking. As expected, strain magnitudes were much lower (by 67.9%) during swimming than during walking. However, the loading regime of the femur

Abstract. Limb bone morphology often correlates with functional demands placed on animals by the environment. Comparisons of limb bone allometry in functionally divergent turtle taxa indicate highly specialized lineages show extensive flattening of the humerus. In Sea Turtles this contributes to flipper-shaped forelimb morphology that facilitates lift-based swimming (i.e., underwater flight). In contrast robust humeri and femora in terrestrial tortoises may reflect specializations for resisting high torsional loads during terrestrial walking and digging. However, it is unknown whether allometric patterns of ecomorphological divergence can be detected among more closely related lineages within clades that encompass species with diverse ecological habits. To test whether limb bone size and shape vary among closely related taxa that live in divergent habitats, we used phylogenetic comparative methods to assess scaling patterns and overall morphology of the humerus and femur of 27 emydi...

Reproduction is linked to a plethora of costs in gravid females, not least of which is a reduction in locomotor performance. Locomotor constraints due to gravidity are apparent across aquatic, terrestrial, and arboreal habitats. Decrements to speed and maneuverability are the most often cited performance consequences of gravidity, regardless of habitat. Arboreal habitats present additional challenges, as they often are composed of unstable and varying substrates that affect locomotor performance. Many arboreal taxa exhibit morphological adaptations, such as grasping extremities and tails, that function to aid in stability during locomotion. Tail length has been found to correlate with lifestyle: arboreal mammals tend to have relatively longer tails compared with terrestrial counterparts. Balancing on a limb is hard on its own, but when combined with increased mass and shifts in center of mass due to pregnancy, it becomes even more challenging. However, few studies have explored the ...

Several terrestrial vertebrate clades include lineages that have evolved nearly exclusive use of aquatic habitats. In many cases, such transitions are associated with the evolution of flattened limbs that are used to swim via dorsoventral flapping. Such changes in shape may have been facilitated by changes in limb bone loading in novel aquatic environments. Studies on limb bone loading in turtles found that torsion is high relative to bending loads on land, but reduced compared to bending during aquatic rowing. Release from torsion among rowers could have facilitated the evolution of hydrodynamically advantageous flattened limbs among aquatic species. Because rowing is regarded as an intermediate locomotor stage between walking and flapping, rowing species might show limb bone flattening intermediate between the tubular shapes of walkers and the flattened shapes of flappers. We collected measurements of humeri and femora from specimens representing four functionally divergent turtle clades: sea turtles (marine flappers), softshells (specialized freshwater rowers), emydids (generalist semiaquatic rowers), and tortoises (terrestrial walkers). Patterns of limb bone scaling with size were compared across lineages using phylogenetic comparative methods. Although rowing taxa did not show the intermediate scaling patterns we predicted, our data provide other functional insights. For example, flattening of sea turtle humeri was associated with positive allometry (relative to body mass) for the limb bone diameter perpendicular to the flexion‐extension plane of the elbow. Moreover, softshell limb bones exhibit positive allometry of femoral diameters relative to body mass, potentially helping them maintain their typical benthic position in water by providing additional weight to compensate for shell reduction. Tortoise limb bones showed positive allometry of diameters, as well as long humeri, relative to body mass, potentially reflecting specializations for resisting loads associated with digging. Overall, scaling patterns of many turtle lineages appear to correlate with distinctive behaviors or locomotor habits.

During evolutionary reinvasions of water by terrestrial vertebrates, ancestrally tubular limb bones often flatten to form flippers. Differences in skeletal loading between land and water might have facilitated such changes. In turtles, femoral shear strains are significantly lower during swimming than during walking, potentially allowing a release from loads favoring tubular shafts. However, flipper-like morphology in specialized tetrapod swimmers is most accentuated in the forelimbs. To test if the forelimbs of turtles also experience reduced torsional loading in water, we compared strains on the humerus of river cooters (Pseudemys concinna) between swimming and terrestrial walking. Humeral shear strains are also lower during swimming compared to terrestrial walking; however, this appears to relate to reduction in overall strain magnitudes, rather than a specific reduction in twisting. These results indicate that shear strains show similar reductions between swimming and walking fo...

Specialization for a new habitat often entails a cost to performance in the ancestral habitat. Although aquatic lifestyles are ancestral among extant cryptodiran turtles, multiple lineages, including tortoises (Testudinidae) and emydid box turtles (genus Terrapene), independently specialized for terrestrial habitats. To what extent is swimming function retained in such lineages despite terrestrial specialization? Because tortoises diverged from other turtles over 50 Ma, but box turtles did so only 5 Ma, we hypothesized that swimming kinematics for box turtles would more closely resemble those of aquatic relatives than those of tortoises. To test this prediction, we compared high-speed video of swimming Russian tortoises (Testudo horsfieldii), box turtles (Terrapene carolina) and two semi-aquatic emydid species: sliders (Trachemys scripta) and painted turtles (Chrysemys picta). We identified different kinematic patterns between limbs. In the forelimb, box turtle strokes most resemble...

Members of several terrestrial vertebrate lineages have returned to nearly exclusive use of aquatic habitats. These transitions were often accompanied by changes in skeletal morphology, such as flattening of limb bone shafts. Such morphological changes might be correlated with the exposure of limb bones to altered loading. Though the environmental forces acting on the skeleton differ substantially between water and land, no empirical data exist to quantify the impact of such differences on the skeleton, either in terms of load magnitude or regime. To test how locomotor loads change between water and land, we compared in vivo strains from femora of turtles ( Trachemys scripta ) during swimming and terrestrial walking. As expected, strain magnitudes were much lower (by 67.9%) during swimming than during walking. However, the loading regime of the femur also changed between environments: torsional strains are high during walking, but torsion is largely eliminated during swimming. Chang...

Though ultimately descended from terrestrial amniotes, turtles have deep roots as an aquatic lineage and are quite diverse in the extent of their aquatic specializations. Many taxa can be viewed as “on the fence” between aquatic and terrestrial realms, whereas others have independently hyperspecialized and moved “all in” to aquatic habitats. Such differences in specialization are reflected strongly in the locomotor system. We have conducted several studies to evaluate the performance consequences of such variation in design, as well as the mechanisms through which specialization for aquatic locomotion is facilitated in turtles. One path to aquatic hyperspecialization has involved the evolutionary transformation of the forelimbs from rowing, tubular limbs with distal paddles into flapping, flattened flippers, as in sea turtles. Prior to the advent of any hydrodynamic advantages, the evolution of such flippers may have been enabled by a reduction in twisting loads on proximal limb bones that accompanied swimming in rowing ancestors, facilitating a shift from tubular to flattened limbs. Moreover, the control of flapping movements appears related primarily to shifts in the activity of a single forelimb muscle, the deltoid. Despite some performance advantages, flapping may entail a locomotor cost in terms of decreased locomotor stability. However, other morphological specializations among rowing species may enhance swimming stability. For example, among highly aquatic pleurodiran turtles, fusion of the pelvis to the shell appears to dramatically reduce motions of the pelvis compared to freshwater cryptodiran species. This could contribute to advantageous increases in aquatic stability among predominantly aquatic pleurodires. Thus, even within the potential constraints of a body plan in which the body is encased by a shell, turtles exhibit diverse locomotor capacities that have enabled diversification into a wide range of aquatic habitats.

Habitats vary in temperature both spatially and temporally.  Variation in thermal habitat introduces challenges to organisms and may reduce fitness unless organisms can physiologically adjust to such changes.  Theory predicts that thermal variability should influence the capacity for acclimation such that increased variation should favor a reduction in the thermal sensitivity of physiological traits.  IN this study, we investigated acclimation to constant and variable conditions in populations of the salamander Desmognathus brimleyorum from the Ouachita Mountains of Arkansas, USA.  We exposed salamanders to constant and variable temperature regimes for 8 weeks in the laboratory.  We then tested salamanders for acclimation of thermal tolerance, and the thermal sensitivities of swimming performance and standard metabolic rate.  Our results indicate limited capacity for thermal acclimation to constant and variable conditions in D. brimleyorum.  Instead, variation in physiological traits is dominated by differences among populations.  Population differences do not appear to be correlated with observed variation in the thermal conditions of the streams, but are likely a consequence of structural and ecological differences.  Due to the mixed support for theoretical predictions for acclimation to thermal environments, further consideration should be given to revising and expanding current theoretical models.

Specialization for a new habitat often entails a cost to performance in the ancestral habitat. Although aquatic lifestyles are ancestral among extant cryptodiran turtles, multiple lineages, including tortoises (Testudinidae) and emydid box turtles (genus <i>Terrapene</i>), independently specialized for terrestrial habitats. To what extent is swimming function retained in such lineages despite terrestrial specialization? Because tortoises diverged from other turtles over 50 Ma, but box turtles did so only 5 Ma, we hypothesized that swimming kinematics for box turtles would more closely resemble those of aquatic relatives than those of tortoises. To test this prediction, we compared high-speed video of swimming Russian tortoises (<i>Testudo horsfieldii</i>), box turtles (<i>Terrapene carolina</i>) and two semi-aquatic emydid species: sliders (<i>Trachemys scripta</i>) and painted turtles (<i>Chrysemys picta</i>). We identified different kinematic patterns between limbs. In the forelimb, box turtle strokes most resemble those of tortoises; for the hindlimb, box turtles are more similar to semi-aquatic species. Such patterns indicate functional convergence of the forelimb of terrestrial species, whereas the box turtle hindlimb exhibits greater retention of ancestral swimming motions.

Supplemental tables and figures. Includes the following: mean values and standard errors of forelimb kinematic variables (Table S1), mean values and standard errors of hindlimb kinematic variables (Table S2), PC loadings from a principal components analysis of forelimb swimming kinematics (Table S3), PC loadings from a principal components analysis of hindlimb swimming kinematics (Table S4), Euclidian distances for forelimb kinematics (Table S5), Euclidian distances for hindlimb kinematics (Table S6), P-values from Tukey's pair-wise comparisons of forelimb kinematic variables (Table S7), P-values from Tukey's pair-wise comparisons of hindlimb kinematic variables (Table S8), ventral and lateral views of limb kinematics (Figure S1), and limb morphology (Figure S2).