Papers by Kazi Ahsan Habib, PhD
Journal of Asiatic Society of Bangladesh, 2019
Genus Upeneus distributed in Indo-Pacific, South Africa, north to southern Japan and south to New... more Genus Upeneus distributed in Indo-Pacific, South Africa, north to southern Japan and south to New Caledonia. Although there is difficulty in identifying accurately goatfish solely on the basis of morphology, U. vittatus and U. supravittatus were confirmed by both using morphological characters and DNA barcoding cytochrome oxidase I subunit (COI) approach for the first time in St. Martin's Island, Bay of Bengal, Bangladesh. Increased number of species of the genus Upeneus was found in Bangladesh from 3 to 5 and extended the distribution range.
Bangladesh Journal of Fisheries, 2020
A new record of Plectropomus pessuliferus (Serranidae: Epinephelinae) was documented based ... more A new record of Plectropomus pessuliferus (Serranidae: Epinephelinae) was documented based on morphological characters and DNA barcoding. The species was collected during a regular survey for making an inventory of reef associated fishes in Saint Martin`s Island, Bangladesh. This is the first report of roving coral grouper from the marine waters of Bangladesh validated by morpho-meristic analysis and DNA barcoding. This is also the first report from the northern Bay of Bengal.
Bangladesh Journal of Fisheries, 2020
A checklist of the marine fishes of Bangladesh is presented with their scientific, common and Ban... more A checklist of the marine fishes of Bangladesh is presented with their scientific, common and Bangla or local names. The global IUCN Red List catagories of these species are also provided. This inventory of the marine fish species is compiled from different major and valid published scientific papers, reports and books published within last 50 years from 1970 to 2020. The directory covers a total of 740 species belonging to 389 Genera of 145 Families and 30 Orders. Among the fish species, 53.38% are exclusively marine and 46.62% are found in both brackish and marine water. Besides, 296 species of fishes are reef associated and 204 of these are recorded from the Saint Martin's Island. Further, 271 species of brakishwater and/or marine fishes are commonly observed in the Sundarbans mangrove ecosystem and its adjacent sea area. About 7% of the total marine fishes of Bangladesh are identified as threatened as per global IUCN Red List. However, the conservation status of the marine fish species of Bangladesh has not yet been assessed locally by IUCN which is essential. The updated checklist will constitute the reference inventory of marine fishes of the coastal and maritime area of the country.
Rupsha is one of the most famous rivers in Bangladesh and endures country's important multi-speci... more Rupsha is one of the most famous rivers in Bangladesh and endures country's important multi-species commercial fishery. The present study was conducted to assess the operation of used fishing gears of the fishermen in the Rupsha River during the period from February 2011 to January, 2012. A wide variety of fishing gears were operated throughout the year in the study area for commercial fishing. A total of 2 gill net, 1 fixed purse net, 1 cast net, 1 dip net, 2 traps, 2 hooks and lines and 2 wounding gears were found in the study area. But a number of the fishing gears was found to catch fish irrespective of their size or species and destroys the habitat of the wild species. An awareness or training program should be conducted to the fishermen to create awareness of the long-term effects of different fishing gears and to impart knowledge of fishing laws.
Sibsa River is a significant and familiar river in southwest Bangladesh in terms of fish producti... more Sibsa River is a significant and familiar river in southwest Bangladesh in terms of fish production and source of income for many fishermen living around it. The present study was planned to assess the current fish diversity status of this river. Fish samples were collected fortnightly from six sampling stations of the Sibsa River at Khulna district in SouthWestern Bangladesh during May 2011 to April 2012. A total of 61 species of fish under 10 orders and 27 families were recorded. Perciformes were most leading order constituting 42.62% of the total fish population followed by Siluriformes (11.48%), Clupeiformes (9.84%), Pleuronectiformes (3.29%), Synbranchiformes (3.29%), Osteoglossiformes (1.64%), Beloniformes (1.64%), Mugiliformes (4.92%), Cypriniformes (16.39%) and Tetraodontiformes (4.92%). Fishes in this river are seriously affected by the various kinds of human development interventions and activities. Under this circumstance, a better understanding of the diversity and distribution of fishes in the Sibsa River is urgently needed.
Stinging catfish (Heteropneustes fossilis) is an important aquaculture fish species in Bangladesh... more Stinging catfish (Heteropneustes fossilis) is an important aquaculture fish species in Bangladesh with high nutritive value. Three microsatellite loci (Cba02, Cba19 and Cba20) were tested to study the genetic variation in this species. Samples were collected from two natural populations namely Mohongonj and Narsingdi and from a hatchery population located in Mymensingh of Bangladesh. All the three loci were found to be polymorphic (P) in the three studied populations. The average observed heterozygosity (H) value was 95 o the highest in Narsingdi population (0.80) followed by the Hatchery (0.70) and the Mohongonj population (0.67). Only Mohongonj population was significantly deviated from Hardy-Weinberg Equilibrium in one locus. The population differentiation value (F) between all the population pairs was insignificant. The highest ST genetic distance value (D = 0.47) was found between the Mohongonj and Hatchery populations while the smallest value (D = 0.34) was found between the Mohongonj and Narsingdi as well as Narsingdi and Hatchery populations. The UPGMA dendrogram based on genetic distance resulted in two major clusters: Hatchery stock alone is in one cluster and the remaining two natural stocks are in other cluster. The study also revealed that the Clarias batrachus microsatellites could be effectively used in the assessment of genetic structure of the stinging catfish H. fossilis
The experiment was conducted to determine the effect of seasonal variation on growth
performance ... more The experiment was conducted to determine the effect of seasonal variation on growth
performance of Cyprinus carpio fry in six earthen ponds during December 2011 to June 2012
covering winter (WS) and summer season (SS). Stocking density was 6250 per decimal with a
mean weight of 0.1920.002 (g). This study was done with three replications for each season.
During the WS, the mean values of water parameters were temperature 16.78±2.17,
transparency 32.67±1.9 cm, dissolved oxygen 5.882.18 mg/l, pH 8.240.49, total alkalinity
184.7222.72, and ammonia nitrogen 0.210.05 whereas the mean value of water parameters in
SS were temperature 30.56±1.51, transparency 30.61±1.71cm, dissolved oxygen 4.31.37 mg/l,
pH 8.330.24, total alkalinity 274.956.73 mg/l, and ammonia nitrogen 0.160.05 mg/l. Mean
values of survival and specific growth rates were 58.940.95 and 2.450.03 in WS and
67.854.27 and 4.001.03, in SS respectively. Mean gross and net productions in WS were
1581.9471.55 kg ha-1 60 d-1 and 1285.0369.84 kg ha-1 60 d-1 and, 4262.74147.81 kg ha-1 60
d-1 and 3964.74145.74 kg ha-1 60 d-1, in SS respectively. Therefore, it can be concluded that
mirror carp fry production was better in SS than in WS.
Different growth and production parameters of cage cultured Vietnamese climbing perch
(Anabas tes... more Different growth and production parameters of cage cultured Vietnamese climbing perch
(Anabas testudineus, Bloach), locally known as koi, were studied. Fishes were reared in bamboo framed net
cages under three different stocking densitiesina single experimental culture cycle of 4 months (July to October,
2013). The experimental earthen pond at Sher-e-Bangla Agricultural University (SAU) campus, Dhaka, was
installed with a total of nine 1m3 (1m x 1m x 1m) cages; wherein 100 fish m 3, 150 fish m 3and 200 fish m 3 were
set as the different stocking densities demarked as treatment T1, T2 and T3 respectively with three replications
each. Mean initial length and weight of the fries were 4.21± 0.114, 4.42± 0.023 and 4.51± 0.047 cm for T1, T2 and
T3, respectively. Mean initial weight for the same treatments were 1.62 ± 0.089, 1.54 ± 0.045 and 1.60 ± 0.0097 g,
respectively. Whereas the mean harvesting length and weight for T1, T2 and T3 recorded were 16.63± 0.119,
16.12± 0.039, 16.67± 0.093 cmand 105.54±1.84, 118.60±2.535, 112.49±2.10 g, respectively. T2 showed the highest
length and weight gain after harvesting (P<0.05). The specific growth rate of T2 was found significantly
different among treatments and again T2 resulting the highest figure of 3.60±0.003 (%). The same treatment had
the highest survival rate of 62.66 ± 4.66 (%) with a mean of 57.44±2.36 (%). However, no significant difference
among treatments was observed for survival rate (P>0.05). Finally, the gross yield (kgm 3) was 7.929±0.92 in
T2 (P<0.05) which was highest among all the treatments and T2 returned back lowest production of 3.39 ± 0.307
kg m 3 which was significantly lower than the yield found in remaining treatments.The gross revenue earned
from selling of fish at a price of 220 Tk kg 1 were 747.98 ± 67.54, 1744.5 ± 203.6 and 1665.1 ± 187.7 Tk m 3 in T ,
1
T2 and T3, respectively. Whereasthe values,in terms of net profit, stood at 418.3 ± 141.16, 779.0 ± 170.73 and
62.00 ± 43.40 Tk m 3 for the same treatments, respectively. Therefore, the cage with 150 fishes (T ) showed
2
promising growth, yield and survival rate which could be recommended to adopt. However, more trials are
suggested to optimize the stocking density and feeding regime for better production performance and profit.
The study was conducted to recover parasitic fungi associated with diseased
fishes. Fishes were c... more The study was conducted to recover parasitic fungi associated with diseased
fishes. Fishes were collected from different water bodies like traditional
culture pond, commercial fish farm and wild water bodies in different
locations of Mymensingh district during. For isolation and identification of
the collected fungi, a small piece of tissue from the lesions of fishes or
mycelium fragments teased from lesion was kept in GP-Pen-Ox broth at
25oC for 10 h. Emerging hyphal strands from this broth were collected and
cultured respectively in GP-Pen-Strep agar, GP-agar and finally in GP-broth.
Collected hyphae from hyphal mat of GP-broth were used for observation of
the hyphal characteristics under microscope and for sporulation.
Identification of fungi was accomplished following the asexual
characteristics of fungal hyphae and the characteristics of sporulation and
spore. The fungi that were isolated and identified are Achlya spp., Aspergillus
spp. and Exophiala spp. Achlya spp. were isolated from the fish species
Anabus testudineus, Channa punctatus and Colisa fasciata; Aspergillus from
Oreochromes niloticus and Exophiala from Puntius gonionotus.
The study was conducted to investigate the socio-economic profiles of the fishermen of river Talm... more The study was conducted to investigate the socio-economic profiles of the fishermen of river Talma in Panchagarh
district in the Northern part of Bangladesh. Pertinent information was collected from fifty randomly selected fishermen from
the study area during the period of eight months from March to October, 2014. In the study area the fishermen age range from
20 to 66 years. It was found that most of the fishermen (58%) illiterate though a small proportion completed primary level of
education. Among them Muslims were 86% and Hindus 14%. About 68% of the fishermen had tin shed with bamboo house,
while 26% and 6% of them had tin shed with tin and straw house, respectively. Annual income of the fishermen ranged from
35001-55000 BDT and they had large family size (member above 7) constituted 36%. Forty eight percent of the fishermen
used their own tube-well, while 40% and 12% used government and neighbors’ tube-well, respectively. About 80% of the
fishermen used kacha toilets and 16% semi pakka toilets and 6% of the fishermen had no sanitary facilities. In the study area,
86% of the fishermen were far from power plants and the rest of them had (14%) access to electricity. Majority (46%) of the
fishermen was landless, while 38% had 1-20 decimal lands and 16% had above 20 decimal lands and significant proportion of
fishermen depend upon village doctors (64%) for their health facilities. Seventy six percent of fishermen were engaged in
fishing as their main occupation, 16% in agriculture and 8% in daily labor. The study revealed that the fishermen of Talma
River were mostly illiterate, poor income, lack of training exposure and lack of awareness about their health facilities and
sanitation. So, provide soft term loan and building people awareness have been suggested to improve the livelihood status of
fishermen in Talma River.
The study was conducted to investigate the present status of fish biodiversity and abundance of f... more The study was conducted to investigate the present status of fish biodiversity and abundance of fish fauna
of an Indo-Bangladesh common river Talma in Northern part of Bangladesh. The study was conducted
from March to October, 2014. A total of 56 species of fishes have been recorded belonging to eight
orders, 21 families and 37 genera. Cypriniformes was the most diversified fish group in terms of both
number of species and individuals observed. Among 56 species, 32 species under the order
Cypriniformes, nine species of Siluriformes, five species of Perciformes, three species of Channiformes,
three species of Synbranchiformes, one species of Beloniformes, one species of Clupeiformes and two
species of Decapoda were found. The study revealed that 32.14% of fish species were threatened. Among
the threatened species, 12.5% were vulnerable, 16.07% endangered and 3.57% were critically
endangered. Overall values of diversity, richness and evenness indices were found 1.42, 6.64 and 0.86,
respectively. From the investigation, it was found that the fish biodiversity of the Talma River has
declined day by day due to over exploitation, natural causes (such as siltation, flooding, drought and
calamities), construction of obstacle for fish migration and lack of public awareness.
The population genetic structure and historical
demography of spotty belly greenling, Hexagrammos... more The population genetic structure and historical
demography of spotty belly greenling, Hexagrammos agrammus, which has limited distribution in the Northwest Pacific, was assessed with partial sequences of the mitochondrial DNA (mtDNA) cytochrome b and the control region (D-loop). A total of 103 individuals were collected from four sites located at the Korea Strait (Southern coast of Korea) and the East coast of Korea and two places in the Pacific coast of Japan. For all the populations, nucleotide diversities were low (0.006 - 0.009) while the haplotype
diversities were as high as 0.92 to 0.97, indicating that the fish has
undergone a recent population expansion after experiencing
bottleneck. Star-shaped patterns of haplotype networks as well as the significant negative values of Tajima’s D and Fu’s FS corroborate the recent population expansion. Mismatch distribution analysis reveals that the demographic expansion of the species started
during the 2nd half of the Middle Pleistocene Series approximately 141,000 - 406,000 years ago. Hierarchical analysis of molecular variance (AMOVA), the pairwise population statistics (FST), and the exact test of haplotype differentiation demonstrate no significant genetic differentiation among populations investigated, suggesting
that spotty belly greenling is panmictic in the East Sea and the
Pacific coast of Japan.
Stinging catfish (Heteropneustes fossilis) is an important aquaculture fish species in Bangladesh... more Stinging catfish (Heteropneustes fossilis) is an important aquaculture fish species in Bangladesh with
high nutritive value. Three microsatellite loci (Cba02, Cba19 and Cba20) were tested to study the genetic
variation in this species. Samples were collected from two natural populations namely Mohongonj and
Narsingdi and from a hatchery population located in Mymensingh of Bangladesh. All the three loci were found
to be polymorphic (P95) in the three studied populations. The average observed heterozygosity (H o) value was
the highest in Narsingdi population (0.80) followed by the Hatchery (0.70) and the Mohongonj population
(0.67). Only Mohongonj population was significantly deviated from Hardy-Weinberg Equilibrium in one locus.
The population differentiation value (FST) between all the population pairs was insignificant. The highest
genetic distance value (D = 0.47) was found between the Mohongonj and Hatchery populations while the
smallest value (D = 0.34) was found between the Mohongonj and Narsingdi as well as Narsingdi and Hatchery
populations. The UPGMA dendrogram based on genetic distance resulted in two major clusters: Hatchery stock
alone is in one cluster and the remaining two natural stocks are in other cluster. The study also revealed that
the Clarias batrachus microsatellites could be effectively used in the assessment of genetic structure of the
stinging catfish H. fossilis
Two nuclear microsatellite DNA loci along with partial sequences of mitochondrial DNA
tRNAPRO-D-l... more Two nuclear microsatellite DNA loci along with partial sequences of mitochondrial DNA
tRNAPRO-D-loop region were analyzed to assess the genetic diversity and population genetic structure
of spottybelly greenling (Hexagrammos agrammus). A total of 85 individuals were investigated from
two different locations at the East and the South coasts of Korea which are Imwonjin (IM) and
Tongyeong (TN), respectively. In the analysis of genetic diversity, nucleotide diversities were low
showing 0.01 whereas haplotype diversities were as high as 0.92 and 0.97 in IM and TN populations,
respectively indicating population bottleneck after rapid growth of these populations. No significant
genealogical branches or clusters were recognized on the neighbor-joining phylogenetic tree. Pairwise
population statistics FST and the exact test of population differentiation from the analysis of
microsatellite DNA loci demonstrated no significant genetic difference between two populations
investigated in the Korean coasts. In addition, significant deviations from Hardy-Weinberg equilibrium
and heterozygote deficiencies were found from the microsatellite DNA loci. The results of the study
will help to make a plan for fisheries management of the species.
Fat greenling (Hexagrammos otakii) is an important commercial
fish in the Northwestern Pacific, b... more Fat greenling (Hexagrammos otakii) is an important commercial
fish in the Northwestern Pacific, being distributed along
the coastal waters of the East/Japan Sea and the Yellow Sea.
To investigate population genetic structure and demographic
history of this species, one hundred and fifty five individuals
were collected from five localities in the distribution range
of the species and sequence variations in the mitochondrial
genes COI, COIII-ND3-ND4L, and cytochrome b were
examined. For all the genes in every sampling location, the
nucleotide diversities were very low (0.001 ~ 0.005) although
the haplotype diversities were relatively high, 0.55 ~ 0.81 for
COI, 0.79 ~ 0.84 for COIII-ND3-ND4L, and 0.95 ~ 0.97 for
cytochrome b. Hierarchical analysis of molecular variance
(AMOVA), the conventional population statistic FST, and exact
test of population differentiation revealed no significant
genetic structuring among the samples, indicating that fat
greening is panmictic throughout the range of its distribution.
Neutrality tests such as Tajima’s D and Fu’s FS statistics and
mismatch distribution analyses suggested that fat greening has
undergone the demographic history of population expansion
during the late Pleistocene period approximately 91,000 ~
327,000 years ago. The star-burst patterns of haplotype networks
and low nucleotide diversities also indicated recent population
expansion. These results help establish the fisheries
management strategy for fat greenling in the Northwestern
Pacific.
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Papers by Kazi Ahsan Habib, PhD
performance of Cyprinus carpio fry in six earthen ponds during December 2011 to June 2012
covering winter (WS) and summer season (SS). Stocking density was 6250 per decimal with a
mean weight of 0.1920.002 (g). This study was done with three replications for each season.
During the WS, the mean values of water parameters were temperature 16.78±2.17,
transparency 32.67±1.9 cm, dissolved oxygen 5.882.18 mg/l, pH 8.240.49, total alkalinity
184.7222.72, and ammonia nitrogen 0.210.05 whereas the mean value of water parameters in
SS were temperature 30.56±1.51, transparency 30.61±1.71cm, dissolved oxygen 4.31.37 mg/l,
pH 8.330.24, total alkalinity 274.956.73 mg/l, and ammonia nitrogen 0.160.05 mg/l. Mean
values of survival and specific growth rates were 58.940.95 and 2.450.03 in WS and
67.854.27 and 4.001.03, in SS respectively. Mean gross and net productions in WS were
1581.9471.55 kg ha-1 60 d-1 and 1285.0369.84 kg ha-1 60 d-1 and, 4262.74147.81 kg ha-1 60
d-1 and 3964.74145.74 kg ha-1 60 d-1, in SS respectively. Therefore, it can be concluded that
mirror carp fry production was better in SS than in WS.
(Anabas testudineus, Bloach), locally known as koi, were studied. Fishes were reared in bamboo framed net
cages under three different stocking densitiesina single experimental culture cycle of 4 months (July to October,
2013). The experimental earthen pond at Sher-e-Bangla Agricultural University (SAU) campus, Dhaka, was
installed with a total of nine 1m3 (1m x 1m x 1m) cages; wherein 100 fish m 3, 150 fish m 3and 200 fish m 3 were
set as the different stocking densities demarked as treatment T1, T2 and T3 respectively with three replications
each. Mean initial length and weight of the fries were 4.21± 0.114, 4.42± 0.023 and 4.51± 0.047 cm for T1, T2 and
T3, respectively. Mean initial weight for the same treatments were 1.62 ± 0.089, 1.54 ± 0.045 and 1.60 ± 0.0097 g,
respectively. Whereas the mean harvesting length and weight for T1, T2 and T3 recorded were 16.63± 0.119,
16.12± 0.039, 16.67± 0.093 cmand 105.54±1.84, 118.60±2.535, 112.49±2.10 g, respectively. T2 showed the highest
length and weight gain after harvesting (P<0.05). The specific growth rate of T2 was found significantly
different among treatments and again T2 resulting the highest figure of 3.60±0.003 (%). The same treatment had
the highest survival rate of 62.66 ± 4.66 (%) with a mean of 57.44±2.36 (%). However, no significant difference
among treatments was observed for survival rate (P>0.05). Finally, the gross yield (kgm 3) was 7.929±0.92 in
T2 (P<0.05) which was highest among all the treatments and T2 returned back lowest production of 3.39 ± 0.307
kg m 3 which was significantly lower than the yield found in remaining treatments.The gross revenue earned
from selling of fish at a price of 220 Tk kg 1 were 747.98 ± 67.54, 1744.5 ± 203.6 and 1665.1 ± 187.7 Tk m 3 in T ,
1
T2 and T3, respectively. Whereasthe values,in terms of net profit, stood at 418.3 ± 141.16, 779.0 ± 170.73 and
62.00 ± 43.40 Tk m 3 for the same treatments, respectively. Therefore, the cage with 150 fishes (T ) showed
2
promising growth, yield and survival rate which could be recommended to adopt. However, more trials are
suggested to optimize the stocking density and feeding regime for better production performance and profit.
fishes. Fishes were collected from different water bodies like traditional
culture pond, commercial fish farm and wild water bodies in different
locations of Mymensingh district during. For isolation and identification of
the collected fungi, a small piece of tissue from the lesions of fishes or
mycelium fragments teased from lesion was kept in GP-Pen-Ox broth at
25oC for 10 h. Emerging hyphal strands from this broth were collected and
cultured respectively in GP-Pen-Strep agar, GP-agar and finally in GP-broth.
Collected hyphae from hyphal mat of GP-broth were used for observation of
the hyphal characteristics under microscope and for sporulation.
Identification of fungi was accomplished following the asexual
characteristics of fungal hyphae and the characteristics of sporulation and
spore. The fungi that were isolated and identified are Achlya spp., Aspergillus
spp. and Exophiala spp. Achlya spp. were isolated from the fish species
Anabus testudineus, Channa punctatus and Colisa fasciata; Aspergillus from
Oreochromes niloticus and Exophiala from Puntius gonionotus.
district in the Northern part of Bangladesh. Pertinent information was collected from fifty randomly selected fishermen from
the study area during the period of eight months from March to October, 2014. In the study area the fishermen age range from
20 to 66 years. It was found that most of the fishermen (58%) illiterate though a small proportion completed primary level of
education. Among them Muslims were 86% and Hindus 14%. About 68% of the fishermen had tin shed with bamboo house,
while 26% and 6% of them had tin shed with tin and straw house, respectively. Annual income of the fishermen ranged from
35001-55000 BDT and they had large family size (member above 7) constituted 36%. Forty eight percent of the fishermen
used their own tube-well, while 40% and 12% used government and neighbors’ tube-well, respectively. About 80% of the
fishermen used kacha toilets and 16% semi pakka toilets and 6% of the fishermen had no sanitary facilities. In the study area,
86% of the fishermen were far from power plants and the rest of them had (14%) access to electricity. Majority (46%) of the
fishermen was landless, while 38% had 1-20 decimal lands and 16% had above 20 decimal lands and significant proportion of
fishermen depend upon village doctors (64%) for their health facilities. Seventy six percent of fishermen were engaged in
fishing as their main occupation, 16% in agriculture and 8% in daily labor. The study revealed that the fishermen of Talma
River were mostly illiterate, poor income, lack of training exposure and lack of awareness about their health facilities and
sanitation. So, provide soft term loan and building people awareness have been suggested to improve the livelihood status of
fishermen in Talma River.
of an Indo-Bangladesh common river Talma in Northern part of Bangladesh. The study was conducted
from March to October, 2014. A total of 56 species of fishes have been recorded belonging to eight
orders, 21 families and 37 genera. Cypriniformes was the most diversified fish group in terms of both
number of species and individuals observed. Among 56 species, 32 species under the order
Cypriniformes, nine species of Siluriformes, five species of Perciformes, three species of Channiformes,
three species of Synbranchiformes, one species of Beloniformes, one species of Clupeiformes and two
species of Decapoda were found. The study revealed that 32.14% of fish species were threatened. Among
the threatened species, 12.5% were vulnerable, 16.07% endangered and 3.57% were critically
endangered. Overall values of diversity, richness and evenness indices were found 1.42, 6.64 and 0.86,
respectively. From the investigation, it was found that the fish biodiversity of the Talma River has
declined day by day due to over exploitation, natural causes (such as siltation, flooding, drought and
calamities), construction of obstacle for fish migration and lack of public awareness.
demography of spotty belly greenling, Hexagrammos agrammus, which has limited distribution in the Northwest Pacific, was assessed with partial sequences of the mitochondrial DNA (mtDNA) cytochrome b and the control region (D-loop). A total of 103 individuals were collected from four sites located at the Korea Strait (Southern coast of Korea) and the East coast of Korea and two places in the Pacific coast of Japan. For all the populations, nucleotide diversities were low (0.006 - 0.009) while the haplotype
diversities were as high as 0.92 to 0.97, indicating that the fish has
undergone a recent population expansion after experiencing
bottleneck. Star-shaped patterns of haplotype networks as well as the significant negative values of Tajima’s D and Fu’s FS corroborate the recent population expansion. Mismatch distribution analysis reveals that the demographic expansion of the species started
during the 2nd half of the Middle Pleistocene Series approximately 141,000 - 406,000 years ago. Hierarchical analysis of molecular variance (AMOVA), the pairwise population statistics (FST), and the exact test of haplotype differentiation demonstrate no significant genetic differentiation among populations investigated, suggesting
that spotty belly greenling is panmictic in the East Sea and the
Pacific coast of Japan.
high nutritive value. Three microsatellite loci (Cba02, Cba19 and Cba20) were tested to study the genetic
variation in this species. Samples were collected from two natural populations namely Mohongonj and
Narsingdi and from a hatchery population located in Mymensingh of Bangladesh. All the three loci were found
to be polymorphic (P95) in the three studied populations. The average observed heterozygosity (H o) value was
the highest in Narsingdi population (0.80) followed by the Hatchery (0.70) and the Mohongonj population
(0.67). Only Mohongonj population was significantly deviated from Hardy-Weinberg Equilibrium in one locus.
The population differentiation value (FST) between all the population pairs was insignificant. The highest
genetic distance value (D = 0.47) was found between the Mohongonj and Hatchery populations while the
smallest value (D = 0.34) was found between the Mohongonj and Narsingdi as well as Narsingdi and Hatchery
populations. The UPGMA dendrogram based on genetic distance resulted in two major clusters: Hatchery stock
alone is in one cluster and the remaining two natural stocks are in other cluster. The study also revealed that
the Clarias batrachus microsatellites could be effectively used in the assessment of genetic structure of the
stinging catfish H. fossilis
tRNAPRO-D-loop region were analyzed to assess the genetic diversity and population genetic structure
of spottybelly greenling (Hexagrammos agrammus). A total of 85 individuals were investigated from
two different locations at the East and the South coasts of Korea which are Imwonjin (IM) and
Tongyeong (TN), respectively. In the analysis of genetic diversity, nucleotide diversities were low
showing 0.01 whereas haplotype diversities were as high as 0.92 and 0.97 in IM and TN populations,
respectively indicating population bottleneck after rapid growth of these populations. No significant
genealogical branches or clusters were recognized on the neighbor-joining phylogenetic tree. Pairwise
population statistics FST and the exact test of population differentiation from the analysis of
microsatellite DNA loci demonstrated no significant genetic difference between two populations
investigated in the Korean coasts. In addition, significant deviations from Hardy-Weinberg equilibrium
and heterozygote deficiencies were found from the microsatellite DNA loci. The results of the study
will help to make a plan for fisheries management of the species.
fish in the Northwestern Pacific, being distributed along
the coastal waters of the East/Japan Sea and the Yellow Sea.
To investigate population genetic structure and demographic
history of this species, one hundred and fifty five individuals
were collected from five localities in the distribution range
of the species and sequence variations in the mitochondrial
genes COI, COIII-ND3-ND4L, and cytochrome b were
examined. For all the genes in every sampling location, the
nucleotide diversities were very low (0.001 ~ 0.005) although
the haplotype diversities were relatively high, 0.55 ~ 0.81 for
COI, 0.79 ~ 0.84 for COIII-ND3-ND4L, and 0.95 ~ 0.97 for
cytochrome b. Hierarchical analysis of molecular variance
(AMOVA), the conventional population statistic FST, and exact
test of population differentiation revealed no significant
genetic structuring among the samples, indicating that fat
greening is panmictic throughout the range of its distribution.
Neutrality tests such as Tajima’s D and Fu’s FS statistics and
mismatch distribution analyses suggested that fat greening has
undergone the demographic history of population expansion
during the late Pleistocene period approximately 91,000 ~
327,000 years ago. The star-burst patterns of haplotype networks
and low nucleotide diversities also indicated recent population
expansion. These results help establish the fisheries
management strategy for fat greenling in the Northwestern
Pacific.
performance of Cyprinus carpio fry in six earthen ponds during December 2011 to June 2012
covering winter (WS) and summer season (SS). Stocking density was 6250 per decimal with a
mean weight of 0.1920.002 (g). This study was done with three replications for each season.
During the WS, the mean values of water parameters were temperature 16.78±2.17,
transparency 32.67±1.9 cm, dissolved oxygen 5.882.18 mg/l, pH 8.240.49, total alkalinity
184.7222.72, and ammonia nitrogen 0.210.05 whereas the mean value of water parameters in
SS were temperature 30.56±1.51, transparency 30.61±1.71cm, dissolved oxygen 4.31.37 mg/l,
pH 8.330.24, total alkalinity 274.956.73 mg/l, and ammonia nitrogen 0.160.05 mg/l. Mean
values of survival and specific growth rates were 58.940.95 and 2.450.03 in WS and
67.854.27 and 4.001.03, in SS respectively. Mean gross and net productions in WS were
1581.9471.55 kg ha-1 60 d-1 and 1285.0369.84 kg ha-1 60 d-1 and, 4262.74147.81 kg ha-1 60
d-1 and 3964.74145.74 kg ha-1 60 d-1, in SS respectively. Therefore, it can be concluded that
mirror carp fry production was better in SS than in WS.
(Anabas testudineus, Bloach), locally known as koi, were studied. Fishes were reared in bamboo framed net
cages under three different stocking densitiesina single experimental culture cycle of 4 months (July to October,
2013). The experimental earthen pond at Sher-e-Bangla Agricultural University (SAU) campus, Dhaka, was
installed with a total of nine 1m3 (1m x 1m x 1m) cages; wherein 100 fish m 3, 150 fish m 3and 200 fish m 3 were
set as the different stocking densities demarked as treatment T1, T2 and T3 respectively with three replications
each. Mean initial length and weight of the fries were 4.21± 0.114, 4.42± 0.023 and 4.51± 0.047 cm for T1, T2 and
T3, respectively. Mean initial weight for the same treatments were 1.62 ± 0.089, 1.54 ± 0.045 and 1.60 ± 0.0097 g,
respectively. Whereas the mean harvesting length and weight for T1, T2 and T3 recorded were 16.63± 0.119,
16.12± 0.039, 16.67± 0.093 cmand 105.54±1.84, 118.60±2.535, 112.49±2.10 g, respectively. T2 showed the highest
length and weight gain after harvesting (P<0.05). The specific growth rate of T2 was found significantly
different among treatments and again T2 resulting the highest figure of 3.60±0.003 (%). The same treatment had
the highest survival rate of 62.66 ± 4.66 (%) with a mean of 57.44±2.36 (%). However, no significant difference
among treatments was observed for survival rate (P>0.05). Finally, the gross yield (kgm 3) was 7.929±0.92 in
T2 (P<0.05) which was highest among all the treatments and T2 returned back lowest production of 3.39 ± 0.307
kg m 3 which was significantly lower than the yield found in remaining treatments.The gross revenue earned
from selling of fish at a price of 220 Tk kg 1 were 747.98 ± 67.54, 1744.5 ± 203.6 and 1665.1 ± 187.7 Tk m 3 in T ,
1
T2 and T3, respectively. Whereasthe values,in terms of net profit, stood at 418.3 ± 141.16, 779.0 ± 170.73 and
62.00 ± 43.40 Tk m 3 for the same treatments, respectively. Therefore, the cage with 150 fishes (T ) showed
2
promising growth, yield and survival rate which could be recommended to adopt. However, more trials are
suggested to optimize the stocking density and feeding regime for better production performance and profit.
fishes. Fishes were collected from different water bodies like traditional
culture pond, commercial fish farm and wild water bodies in different
locations of Mymensingh district during. For isolation and identification of
the collected fungi, a small piece of tissue from the lesions of fishes or
mycelium fragments teased from lesion was kept in GP-Pen-Ox broth at
25oC for 10 h. Emerging hyphal strands from this broth were collected and
cultured respectively in GP-Pen-Strep agar, GP-agar and finally in GP-broth.
Collected hyphae from hyphal mat of GP-broth were used for observation of
the hyphal characteristics under microscope and for sporulation.
Identification of fungi was accomplished following the asexual
characteristics of fungal hyphae and the characteristics of sporulation and
spore. The fungi that were isolated and identified are Achlya spp., Aspergillus
spp. and Exophiala spp. Achlya spp. were isolated from the fish species
Anabus testudineus, Channa punctatus and Colisa fasciata; Aspergillus from
Oreochromes niloticus and Exophiala from Puntius gonionotus.
district in the Northern part of Bangladesh. Pertinent information was collected from fifty randomly selected fishermen from
the study area during the period of eight months from March to October, 2014. In the study area the fishermen age range from
20 to 66 years. It was found that most of the fishermen (58%) illiterate though a small proportion completed primary level of
education. Among them Muslims were 86% and Hindus 14%. About 68% of the fishermen had tin shed with bamboo house,
while 26% and 6% of them had tin shed with tin and straw house, respectively. Annual income of the fishermen ranged from
35001-55000 BDT and they had large family size (member above 7) constituted 36%. Forty eight percent of the fishermen
used their own tube-well, while 40% and 12% used government and neighbors’ tube-well, respectively. About 80% of the
fishermen used kacha toilets and 16% semi pakka toilets and 6% of the fishermen had no sanitary facilities. In the study area,
86% of the fishermen were far from power plants and the rest of them had (14%) access to electricity. Majority (46%) of the
fishermen was landless, while 38% had 1-20 decimal lands and 16% had above 20 decimal lands and significant proportion of
fishermen depend upon village doctors (64%) for their health facilities. Seventy six percent of fishermen were engaged in
fishing as their main occupation, 16% in agriculture and 8% in daily labor. The study revealed that the fishermen of Talma
River were mostly illiterate, poor income, lack of training exposure and lack of awareness about their health facilities and
sanitation. So, provide soft term loan and building people awareness have been suggested to improve the livelihood status of
fishermen in Talma River.
of an Indo-Bangladesh common river Talma in Northern part of Bangladesh. The study was conducted
from March to October, 2014. A total of 56 species of fishes have been recorded belonging to eight
orders, 21 families and 37 genera. Cypriniformes was the most diversified fish group in terms of both
number of species and individuals observed. Among 56 species, 32 species under the order
Cypriniformes, nine species of Siluriformes, five species of Perciformes, three species of Channiformes,
three species of Synbranchiformes, one species of Beloniformes, one species of Clupeiformes and two
species of Decapoda were found. The study revealed that 32.14% of fish species were threatened. Among
the threatened species, 12.5% were vulnerable, 16.07% endangered and 3.57% were critically
endangered. Overall values of diversity, richness and evenness indices were found 1.42, 6.64 and 0.86,
respectively. From the investigation, it was found that the fish biodiversity of the Talma River has
declined day by day due to over exploitation, natural causes (such as siltation, flooding, drought and
calamities), construction of obstacle for fish migration and lack of public awareness.
demography of spotty belly greenling, Hexagrammos agrammus, which has limited distribution in the Northwest Pacific, was assessed with partial sequences of the mitochondrial DNA (mtDNA) cytochrome b and the control region (D-loop). A total of 103 individuals were collected from four sites located at the Korea Strait (Southern coast of Korea) and the East coast of Korea and two places in the Pacific coast of Japan. For all the populations, nucleotide diversities were low (0.006 - 0.009) while the haplotype
diversities were as high as 0.92 to 0.97, indicating that the fish has
undergone a recent population expansion after experiencing
bottleneck. Star-shaped patterns of haplotype networks as well as the significant negative values of Tajima’s D and Fu’s FS corroborate the recent population expansion. Mismatch distribution analysis reveals that the demographic expansion of the species started
during the 2nd half of the Middle Pleistocene Series approximately 141,000 - 406,000 years ago. Hierarchical analysis of molecular variance (AMOVA), the pairwise population statistics (FST), and the exact test of haplotype differentiation demonstrate no significant genetic differentiation among populations investigated, suggesting
that spotty belly greenling is panmictic in the East Sea and the
Pacific coast of Japan.
high nutritive value. Three microsatellite loci (Cba02, Cba19 and Cba20) were tested to study the genetic
variation in this species. Samples were collected from two natural populations namely Mohongonj and
Narsingdi and from a hatchery population located in Mymensingh of Bangladesh. All the three loci were found
to be polymorphic (P95) in the three studied populations. The average observed heterozygosity (H o) value was
the highest in Narsingdi population (0.80) followed by the Hatchery (0.70) and the Mohongonj population
(0.67). Only Mohongonj population was significantly deviated from Hardy-Weinberg Equilibrium in one locus.
The population differentiation value (FST) between all the population pairs was insignificant. The highest
genetic distance value (D = 0.47) was found between the Mohongonj and Hatchery populations while the
smallest value (D = 0.34) was found between the Mohongonj and Narsingdi as well as Narsingdi and Hatchery
populations. The UPGMA dendrogram based on genetic distance resulted in two major clusters: Hatchery stock
alone is in one cluster and the remaining two natural stocks are in other cluster. The study also revealed that
the Clarias batrachus microsatellites could be effectively used in the assessment of genetic structure of the
stinging catfish H. fossilis
tRNAPRO-D-loop region were analyzed to assess the genetic diversity and population genetic structure
of spottybelly greenling (Hexagrammos agrammus). A total of 85 individuals were investigated from
two different locations at the East and the South coasts of Korea which are Imwonjin (IM) and
Tongyeong (TN), respectively. In the analysis of genetic diversity, nucleotide diversities were low
showing 0.01 whereas haplotype diversities were as high as 0.92 and 0.97 in IM and TN populations,
respectively indicating population bottleneck after rapid growth of these populations. No significant
genealogical branches or clusters were recognized on the neighbor-joining phylogenetic tree. Pairwise
population statistics FST and the exact test of population differentiation from the analysis of
microsatellite DNA loci demonstrated no significant genetic difference between two populations
investigated in the Korean coasts. In addition, significant deviations from Hardy-Weinberg equilibrium
and heterozygote deficiencies were found from the microsatellite DNA loci. The results of the study
will help to make a plan for fisheries management of the species.
fish in the Northwestern Pacific, being distributed along
the coastal waters of the East/Japan Sea and the Yellow Sea.
To investigate population genetic structure and demographic
history of this species, one hundred and fifty five individuals
were collected from five localities in the distribution range
of the species and sequence variations in the mitochondrial
genes COI, COIII-ND3-ND4L, and cytochrome b were
examined. For all the genes in every sampling location, the
nucleotide diversities were very low (0.001 ~ 0.005) although
the haplotype diversities were relatively high, 0.55 ~ 0.81 for
COI, 0.79 ~ 0.84 for COIII-ND3-ND4L, and 0.95 ~ 0.97 for
cytochrome b. Hierarchical analysis of molecular variance
(AMOVA), the conventional population statistic FST, and exact
test of population differentiation revealed no significant
genetic structuring among the samples, indicating that fat
greening is panmictic throughout the range of its distribution.
Neutrality tests such as Tajima’s D and Fu’s FS statistics and
mismatch distribution analyses suggested that fat greening has
undergone the demographic history of population expansion
during the late Pleistocene period approximately 91,000 ~
327,000 years ago. The star-burst patterns of haplotype networks
and low nucleotide diversities also indicated recent population
expansion. These results help establish the fisheries
management strategy for fat greenling in the Northwestern
Pacific.