… expert admitted that the enzyme that resulted from that procedure was not nuclease-free … that
Prep 4 contained only a "very small" or "minimal" amount of nucleases, but Promega's …

… Any of a variety of nucleases can be used." (Id. at 32.) The specification goes on to say, "One
of skill in the art can select an appropriate nuclease." (Id.) An endonuclease digests all …

… of the specifications provides that in a different embodiment, "the cleavage means is a
structure-specific nuclease; particularly preferred structure-specific nucleases are thermostable …

… These included the possibility that CRISPR-Cas9 might be degraded by nucleases in eukaryotic …
Specifically, it found that the prior art TALEN and zinc finger nuclease ("ZFN") systems …

… of the specifications provides that in a different embodiment, "the cleavage means is a
structure-specific nuclease; particularly preferred structure-specific nucleases are thermostable …

… I) and, more specifically, modified DNAPs having 5' nuclease but lacking synthetic activity." '543
pat., col. 20, lns. 58-61; '314 pat., col. 18. lns. 9-12. "The ability of 5' nucleases to cleave …

857 F.3d 833 (2017). Karen VANOVER, Plaintiff-Appellant, v. NCO FINANCIAL SERVICES, INC.,
Defendant-Appellee. No. 15-15294. United States Court of Appeals, Eleventh Circuit …

138 S.Ct. 1348 (2018). 584 US 357. SAS INSTITUTE INC., Petitioner v. Andrei IANCU, as
Director, United States Patent and Trademark Office, et al. No. 16-969 …

… 4. The method of claim 1, wherein the detecting step is by a) allele specific hybridization; b) size
analysis; c) sequencing; d) hybridization; e) 5' nuclease digestion; f) single-stranded …

26 Cal.3d 588 (1980). 607 P.2d 924. 163 Cal. Rptr. 132. JUDITH SINDELL, Plaintiff and Appellant,
v. ABBOTT LABORATORIES et al., Defendants and Respondents …