Human toxocariasis, a worldwide parasitic disease, is caused by the larval stage of intestinal ne... more Human toxocariasis, a worldwide parasitic disease, is caused by the larval stage of intestinal nematodes of dogs and cats, namelyToxocara canisandToxocara cati.Human infection occurs by the accidental ingestion of embryonated eggs present in the soil, vegetables or on other contaminated surfaces, as well as via consumption of uncooked paratenic hosts, such as bird meat and giblets. The objective of this study was to evaluate the contamination of soil in public parks and playgrounds in Shiraz using microscopy and molecular methods. A total of 150 soil samples were collected from public parks and playgrounds in various areas of Shiraz, southern Iran. The samples were treated with saturated zinc sulphate solution, andToxocaraspp. eggs were detected by microscopic observation followed by nested polymerase chain reaction (PCR). To differentiateT. canisandT. catieggs from each other, PCR restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS)-rDNA region b...
The study was conducted to determine the sequence variation in two mitochondrial genes, namely cy... more The study was conducted to determine the sequence variation in two mitochondrial genes, namely cytochromecoxidase 1 (pcox1) and NADH dehydrogenase 1 (pnad1) within and among isolates ofToxocara cati,Toxocara canisandToxascaris leonina. Genomic DNA was extracted from 32 isolates ofT. cati, 9 isolates ofT. canisand 19 isolates ofT. leoninacollected from cats and dogs in different geographical areas of Iran. Mitochondrial genes were amplified by polymerase chain reaction (PCR) and sequenced. Sequence data were aligned using the BioEdit software and compared with published sequences in GenBank. Phylogenetic analysis was performed using Bayesian inference and maximum likelihood methods. Based on pairwise comparison, intra-species genetic diversity within Iranian isolates ofT. cati,T. canisandT. leoninaamounted to 0–2.3%, 0–1.3% and 0–1.0% for pcox1 and 0–2.0%, 0–1.7% and 0–2.6% for pnad1, respectively. Inter-species sequence variation among the three ascaridoid nematodes was significantl...
Toxocara is a common nematode of cats in different parts of Iran. Despite the close association o... more Toxocara is a common nematode of cats in different parts of Iran. Despite the close association of cats with human, no attempt has been done so far for molecular identification of this nematode in the country. Therefore, current study was performed on identification of some isolates of Toxocara from stray cats in Shiraz, Fars Province, Southern Iran, based on morphological and molecular approaches, and also determination of intensity of infection. This cross-sectional study was carried out on 30 stray cats trapped from different geographical areas of Shiraz in 2011. Adult male and female worms were recovered from digestive tract after dissection of cats. Morphological features using existing keys and PCR-sequencing of ITS-rDNA region and pcox1 mitochondrial l gene were applied for the delineating the species of the parasites. Eight out of 30 cats (26.7%) were found infected with Toxocara nematodes. All the isolates were confirmed as Toxocara cati based on morphological features and ...
Journal of Mazandaran University of Medical Sciences
Abstract: AbstractBackground and purpose: Visceral leishmaniasis (Kala-azar) is one of the parasi... more Abstract: AbstractBackground and purpose: Visceral leishmaniasis (Kala-azar) is one of the parasitic zoonotic diseases which is caused by Leishmania donovani complex parasites. Thus, the aim of this study is the application of different enzymatic systems for discrimination species and strains visceral leishmaniasis agent. Materials and methods: In this experimental study, reference strains of Leishmania infatum, Leishmania major, Leishmania tropica in addition, the leishmania parasites isolated from bone marrow of subjects and ...
Results Of 186 suspected patients, 104 (56%) cases were infected with CL. PCR results were positi... more Results Of 186 suspected patients, 104 (56%) cases were infected with CL. PCR results were positive in 35 (56.4%) out of 62 smears, among which microscopic examination did not reveal Leishmania amastigotes. Also, Leishmania species isolated from the patients residing in the urban and rural areas were Leishmania tropica and Leishmania major by species-specific primers, respectively. Conclusion Since in some cases the direct smear is reported negative, we suggest using PCR methods on smears in endemic regions.
Journal of Mazandaran University of Medical Sciences, May 15, 2009
Background and purpose: Visceral leishmaniasis (Kala-azar) is one of the parasitic zoonotic disea... more Background and purpose: Visceral leishmaniasis (Kala-azar) is one of the parasitic zoonotic diseases which is caused by Leishmania donovani complex parasites. Thus, the aim of this study is the application of different enzymatic systems for discrimination species and strains visceral leishmaniasis agent. Materials and methods: In this experimental study, reference strains of Leishmania infatum, Leishmania major, Leishmania tropica in addition, the leishmania parasites isolated from bone marrow of subjects and internal organs of dogs ...
Background: The causative agent of visceral leishmaniasis (VL) in Iran is Leishmania infantum (L.... more Background: The causative agent of visceral leishmaniasis (VL) in Iran is Leishmania infantum (L. infantum) (Mediterranean type) and its major reservoir host is the dog. Ob- jective: To compare the serological methods including direct agglutination test (DAT), indirect immunofluorescent-antibody test (IFA) and enzyme-linked immunosorbent as- say (ELISA) for serodiagnosis of endemic strain of L. infantum. Methods: 61 blood samples from
Human toxocariasis, a worldwide parasitic disease, is caused by the larval stage of intestinal ne... more Human toxocariasis, a worldwide parasitic disease, is caused by the larval stage of intestinal nematodes of dogs and cats, namelyToxocara canisandToxocara cati.Human infection occurs by the accidental ingestion of embryonated eggs present in the soil, vegetables or on other contaminated surfaces, as well as via consumption of uncooked paratenic hosts, such as bird meat and giblets. The objective of this study was to evaluate the contamination of soil in public parks and playgrounds in Shiraz using microscopy and molecular methods. A total of 150 soil samples were collected from public parks and playgrounds in various areas of Shiraz, southern Iran. The samples were treated with saturated zinc sulphate solution, andToxocaraspp. eggs were detected by microscopic observation followed by nested polymerase chain reaction (PCR). To differentiateT. canisandT. catieggs from each other, PCR restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS)-rDNA region b...
The study was conducted to determine the sequence variation in two mitochondrial genes, namely cy... more The study was conducted to determine the sequence variation in two mitochondrial genes, namely cytochromecoxidase 1 (pcox1) and NADH dehydrogenase 1 (pnad1) within and among isolates ofToxocara cati,Toxocara canisandToxascaris leonina. Genomic DNA was extracted from 32 isolates ofT. cati, 9 isolates ofT. canisand 19 isolates ofT. leoninacollected from cats and dogs in different geographical areas of Iran. Mitochondrial genes were amplified by polymerase chain reaction (PCR) and sequenced. Sequence data were aligned using the BioEdit software and compared with published sequences in GenBank. Phylogenetic analysis was performed using Bayesian inference and maximum likelihood methods. Based on pairwise comparison, intra-species genetic diversity within Iranian isolates ofT. cati,T. canisandT. leoninaamounted to 0–2.3%, 0–1.3% and 0–1.0% for pcox1 and 0–2.0%, 0–1.7% and 0–2.6% for pnad1, respectively. Inter-species sequence variation among the three ascaridoid nematodes was significantl...
Toxocara is a common nematode of cats in different parts of Iran. Despite the close association o... more Toxocara is a common nematode of cats in different parts of Iran. Despite the close association of cats with human, no attempt has been done so far for molecular identification of this nematode in the country. Therefore, current study was performed on identification of some isolates of Toxocara from stray cats in Shiraz, Fars Province, Southern Iran, based on morphological and molecular approaches, and also determination of intensity of infection. This cross-sectional study was carried out on 30 stray cats trapped from different geographical areas of Shiraz in 2011. Adult male and female worms were recovered from digestive tract after dissection of cats. Morphological features using existing keys and PCR-sequencing of ITS-rDNA region and pcox1 mitochondrial l gene were applied for the delineating the species of the parasites. Eight out of 30 cats (26.7%) were found infected with Toxocara nematodes. All the isolates were confirmed as Toxocara cati based on morphological features and ...
Journal of Mazandaran University of Medical Sciences
Abstract: AbstractBackground and purpose: Visceral leishmaniasis (Kala-azar) is one of the parasi... more Abstract: AbstractBackground and purpose: Visceral leishmaniasis (Kala-azar) is one of the parasitic zoonotic diseases which is caused by Leishmania donovani complex parasites. Thus, the aim of this study is the application of different enzymatic systems for discrimination species and strains visceral leishmaniasis agent. Materials and methods: In this experimental study, reference strains of Leishmania infatum, Leishmania major, Leishmania tropica in addition, the leishmania parasites isolated from bone marrow of subjects and ...
Results Of 186 suspected patients, 104 (56%) cases were infected with CL. PCR results were positi... more Results Of 186 suspected patients, 104 (56%) cases were infected with CL. PCR results were positive in 35 (56.4%) out of 62 smears, among which microscopic examination did not reveal Leishmania amastigotes. Also, Leishmania species isolated from the patients residing in the urban and rural areas were Leishmania tropica and Leishmania major by species-specific primers, respectively. Conclusion Since in some cases the direct smear is reported negative, we suggest using PCR methods on smears in endemic regions.
Journal of Mazandaran University of Medical Sciences, May 15, 2009
Background and purpose: Visceral leishmaniasis (Kala-azar) is one of the parasitic zoonotic disea... more Background and purpose: Visceral leishmaniasis (Kala-azar) is one of the parasitic zoonotic diseases which is caused by Leishmania donovani complex parasites. Thus, the aim of this study is the application of different enzymatic systems for discrimination species and strains visceral leishmaniasis agent. Materials and methods: In this experimental study, reference strains of Leishmania infatum, Leishmania major, Leishmania tropica in addition, the leishmania parasites isolated from bone marrow of subjects and internal organs of dogs ...
Background: The causative agent of visceral leishmaniasis (VL) in Iran is Leishmania infantum (L.... more Background: The causative agent of visceral leishmaniasis (VL) in Iran is Leishmania infantum (L. infantum) (Mediterranean type) and its major reservoir host is the dog. Ob- jective: To compare the serological methods including direct agglutination test (DAT), indirect immunofluorescent-antibody test (IFA) and enzyme-linked immunosorbent as- say (ELISA) for serodiagnosis of endemic strain of L. infantum. Methods: 61 blood samples from
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