Pseudomonas flectens (Johnson 1956), a plant pathogenic bacterium on the pods of the French bean, is not considered to be a member of the genus Pseudomonas sensu stricto. A polyphasic approach that included phenotypic properties and... more
Pseudomonas flectens (Johnson 1956), a plant pathogenic bacterium on the pods of the French bean, is not considered to be a member of the genus Pseudomonas sensu stricto. A polyphasic approach that included phenotypic properties and phylogenetic analysis based on 16S rRNA, rpoB and atpD gene sequences supported the transfer of Pseudomonas flectens, Johnson 1956 to a novel genus in the Enterobacteriaceae family as Phaseolibacter flectens comb. nov. Two strains of Phaseolibacter flectens were studied (ATCC 12775T and LMG 2186). The strains shared 99.8% sequence similarities in their 16SrRNA gene and the housekeeping genes were identical. Phaseolibacter flectens shared 96.6% sequence similarities, or less, with species from different genera in the Enterobacteriaceae family and only 84.7% sequence similarities with Pseudomonas aeruginosa LMG 1242T, demonstrating that it is not related to the genus Pseudomonas. As Phaseolibacter flectens formed an independent phyletic lineage in all the ...
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Summary Floral nectar is regarded as the most important reward available to animal-pollinated plants to attract pollinators. Despite the vast amount of publications on nectar properties, the role of nectar as a natural bacterial habitat... more
Summary Floral nectar is regarded as the most important reward available to animal-pollinated plants to attract pollinators. Despite the vast amount of publications on nectar properties, the role of nectar as a natural bacterial habitat is yet unexplored. To gain a better understanding of bacterial communities inhabiting floral nectar, culture-dependent and-independent (454-pyrosequencing) methods were used. Our findings demonstrate that bacterial communities in nectar are abundant and diverse. Using culturedependent ...