Zulfiqur Ali
Teesside University, Graduate Research School, Department Member
Research Interests:
Research Interests:
Low cost and highly sensitive cavity enhanced absorption detection.
Research Interests:
Research Interests:
Microbioreactor system, with inset the microbioreactor element, and an example cultivation growth profile showing dissolved oxygen, pH and dry cell weight.
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A reusable low cost microfluidic cell culture array device (MCCAD) integrated with a six output concentration gradient generator (cGG) and 4 Â 6 arrays of microchamber elements, addressed by a series of row and columnar pneumatically... more
A reusable low cost microfluidic cell culture array device (MCCAD) integrated with a six output concentration gradient generator (cGG) and 4 Â 6 arrays of microchamber elements, addressed by a series of row and columnar pneumatically actuated normally closed (NC) microvalves was fabricated for cell-based screening of chemotherapeutic compounds. The poly(dimethylsiloxane) (PDMS) device consists of three layers: fluidic, control and membrane which are held by surface contact and made leak-proof by clamping pressure. The NC valves are actuated by a thick PDMS membrane that was created by a novel method based on the self-assembly of PDMS pre-polymer molecules over a denser calcium chloride solution. The membrane actuated the valves reliably and particulates such as alumina particles (3 mm) and MCF-7 cells (20-24 mm) (2 Â 10 5 cells/mL) were flowed through the valves without causing blockage or leakage and consequently avoiding contamination of the different cell culture elements. The MCCAD was cast and assembled in a standard laboratory without specialist equipment and demonstrated for performing quantitative cell-based cytotoxicity assays of pyocyanine on human breast cancer (MCF-7) cells and assessed for toxic effect on human hepatocyte carcinoma (HepG2) cells as an indicator for liver injury. Then, the MCCAD was demonstrated for sequential drug combinatorial screening involving gradient generation of paclitaxel doses followed by treatment with aspirin doses on the viability of MCF-7 cells. The interaction between paclitaxel and aspirin was evaluated by using the Bliss independence predictive model and results showed reasonable agreement with the model. A robust, portable, easily fabricated and low cost device is therefore shown to conveniently carry out culturing of multiple cell lines for high throughput screening of anti-cancer compounds using minimal reagents.
The use of microfluidic bioreactor platforms for cell culturing holds considerable promise for a range of fields which include drug discovery, tissue engineering, bioprocessing optimisation and cell based screening studies. Microfluidic... more
The use of microfluidic bioreactor platforms for cell culturing holds considerable promise for a range of fields
which include drug discovery, tissue engineering, bioprocessing optimisation and cell based screening studies. Microfluidic
bioreactor systems have length scales that are well matched to the physical dimensions of most cells and microorganisms.
In view of this, microfluidic bioreactors have attractive features which make them ideal to study the behaviour of
cells and their internal organisation in their native microenvironment. Due to their small footprint microbioreactor platforms offer a number of advantages over conventional macroscale systems including improved biological function, higher quality cell-based data, reduced volume of reagents, ease of integration and lower cost. This review highlights the basic
concepts, designs and operational requirements of microbioreactors for cell based studies. An illustrative outline of different applications of microbioreactors and some indication of new trends and progress in recent years are provided. Specific
examples of applications of microbioreactors are drawn for cytotoxicity assays, tissue engineering, stem cells, microbial
fermentations, single cell analysis and in vitro fertilisation.
which include drug discovery, tissue engineering, bioprocessing optimisation and cell based screening studies. Microfluidic
bioreactor systems have length scales that are well matched to the physical dimensions of most cells and microorganisms.
In view of this, microfluidic bioreactors have attractive features which make them ideal to study the behaviour of
cells and their internal organisation in their native microenvironment. Due to their small footprint microbioreactor platforms offer a number of advantages over conventional macroscale systems including improved biological function, higher quality cell-based data, reduced volume of reagents, ease of integration and lower cost. This review highlights the basic
concepts, designs and operational requirements of microbioreactors for cell based studies. An illustrative outline of different applications of microbioreactors and some indication of new trends and progress in recent years are provided. Specific
examples of applications of microbioreactors are drawn for cytotoxicity assays, tissue engineering, stem cells, microbial
fermentations, single cell analysis and in vitro fertilisation.
Research Interests:
Research Interests:
Research Interests:
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Research Interests: Artificial Intelligence, Analytical Chemistry, Multivariate Statistics, Fuzzy Logic, Data Analysis, and 14 morePattern Recognition, Process Control, Neural Network, Genetic Algorithm, Sensor Arrays, Multivariate Analysis, Data Visualisation, Electronic Nose, Artificial Intelligent, Gas Sensor, Trend Analysis, Sensor Array, Volatile organic compound, and Selectivity
Research Interests:
Research Interests:
Research Interests: Analytical Chemistry, Fuzzy Logic, Data Analysis, Computational Biology, Chemometrics, and 10 morePrincipal Component Analysis, Pattern Recognition, Radial Basis Function, Quality Control, Multivariate Analysis, Chemical Analysis, Qualitative Analysis, Analyst, Plant Oils, and Backpropagation Algorithm
Research Interests:
Research Interests:
The Lab-on-a-chip systems regarding the terrorist activity for the detection of warfare agents and explosives are discussed. These systems have the potential to provide reliable forensic information in a faster, simpler and cheaper... more
The Lab-on-a-chip systems regarding the terrorist activity for the detection of warfare agents and explosives are discussed. These systems have the potential to provide reliable forensic information in a faster, simpler and cheaper manner. These systems integrate all of the ...
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Research Interests:
Self-assembled monolayers (SAMs) have numerous applications, for example, engine wear inhibitors, surface profiling signal enhancement, nanostructure production, sensor production, and catalysis. The adsorbed SAM structure has a major... more
Self-assembled monolayers (SAMs) have numerous applications, for example, engine wear inhibitors, surface profiling signal enhancement, nanostructure production, sensor production, and catalysis. The adsorbed SAM structure has a major impact on the properties of the outer monolayer surface which dictates the performance and viability of the SAM for individual applications. Substrate growth phases of SAMs have been extensively studied, and two structures have been identified. Initially, a lying down SAM structure is formed that evolves into a standing up structure. It is often critical to know how both structures form as a function of substrate immersion time to be able to design the properties of this structure. The formation of mercaptopropionic acid (MPA) SAMs on gold has been studied. Electrochemical impedance spectroscopy (EIS) was used to measure the adsorption isotherms at five temperatures in the range 4-40 degrees C. Infrared reflectance absorption spectroscopy (IRRAS) was also used, and the results show close agreement. A new monolayer adsorption isotherm is proposed which models SAM structure formation as a function of immersion time, representing all phases of SAM adsorption. This model represents a significant improvement on previous models based on Langmuir and Kisliuk adsorption isotherms that only model the fractional coverage of a surface with a SAM. The new model predicts the optimum immersion time taken for an MPA monolayer on gold to attain a surface saturated with MPA. It accounts for temperature effects on the rate of formation and the degree of monolayer disorder. It has potential for use in other SAM systems and may become the method of choice for modeling many instances of sequential substrate adsorption of two different structures, each of which exhibits different properties, as a function of immersion time.
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Research Interests:
Research Interests:
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The photoreaction of thionine with NAD(P)H leads to the formation of non-fluorescent semi and lecuothionine. This has been previously used for the development of enzymatic assays. We show that the fluorescence of thionine is quenched in... more
The photoreaction of thionine with NAD(P)H leads to the formation of non-fluorescent semi and lecuothionine. This has been previously used for the development of enzymatic assays. We show that the fluorescence of thionine is quenched in the presence of Nocardia corallin. We ...
A process based on a simulation of an industrial float line has been used for deposition of TiO 2 on glass slides. The process is operated at atmospheric pressure rather than under vacuum condition. A variety of organic pre-cursors can be... more
A process based on a simulation of an industrial float line has been used for deposition of TiO 2 on glass slides. The process is operated at atmospheric pressure rather than under vacuum condition. A variety of organic pre-cursors can be used for TiO 2 formation and films of ...
Research Interests:
The photoreaction of thionine with NAD(P)H leads to the formation of non-fluorescent semi and lecuothionine. This has been previously used for the development of enzymatic assays. We show that the fluorescence of thionine is quenched in... more
The photoreaction of thionine with NAD(P)H leads to the formation of non-fluorescent semi and lecuothionine. This has been previously used for the development of enzymatic assays. We show that the fluorescence of thionine is quenched in the presence of Nocardia corallin. We ...
The pattern of responses from a four sensor array have been used for the classification of methanol, propanol, butanol, hexane, heptane and toluene using artificial intelligence (AI) based pattern recognition methods. A feedforward... more
The pattern of responses from a four sensor array have been used for the classification of methanol, propanol, butanol, hexane, heptane and toluene using artificial intelligence (AI) based pattern recognition methods. A feedforward forward network with backpropagation was ...
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Scott, SM, O'Hare, WT and Ali, Z. (2003) 'Fuzzy logic and fuzzy classification techniques', in Cartwright, HM and Sztandera, LM (eds) Soft computing approaches in chemistry. Berlin: Springer Verlag, pp.95. ...... more
Scott, SM, O'Hare, WT and Ali, Z. (2003) 'Fuzzy logic and fuzzy classification techniques', in Cartwright, HM and Sztandera, LM (eds) Soft computing approaches in chemistry. Berlin: Springer Verlag, pp.95. ... There are no files associated with this item. ... Del.icio.us LinkedIn ...
Research Interests:
A process based on a simulation of an industrial float line has been used for deposition of TiO on glass slides. The process is operated at atmospheric pressure rather than under vacuum condition. A variety of organic pre-cursors can be... more
A process based on a simulation of an industrial float line has been used for deposition of TiO on glass slides. The process is operated at atmospheric pressure rather than under vacuum condition. A variety of organic pre-cursors can be used for TiO formation and films of ...
The pattern of responses from a four sensor array have been used for the classification of methanol, propanol, butanol, hexane, heptane and toluene using artificial intelligence (AI) based pattern recognition methods. A feedforward... more
The pattern of responses from a four sensor array have been used for the classification of methanol, propanol, butanol, hexane, heptane and toluene using artificial intelligence (AI) based pattern recognition methods. A feedforward forward network with backpropagation was ...
Research Interests:
Ali, Z., O'Hare, WT and Theaker, BJ (2001) 'Application of a piezoelectric quartz crystal based electronic nose for the sampling of heated edible oil', 8th international symposium on olfaction and the electronic... more
Ali, Z., O'Hare, WT and Theaker, BJ (2001) 'Application of a piezoelectric quartz crystal based electronic nose for the sampling of heated edible oil', 8th international symposium on olfaction and the electronic nose, Washington DC, March 25-30, in Penrose, WR and Stetter, JR ...
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Research Interests:
Research Interests:
P1: SFK/UKS P2: SFK c02 BLBK298-Alasalvar August 5, 2010 15: 16 Trim: 244mm× 172mm 2 Practical evaluation of fish quality by objective, subjective, and statistical testing Cesarettin Alasalvar, John M. Grigor, and Zulfiqur Ali 2.1... more
P1: SFK/UKS P2: SFK c02 BLBK298-Alasalvar August 5, 2010 15: 16 Trim: 244mm× 172mm 2 Practical evaluation of fish quality by objective, subjective, and statistical testing Cesarettin Alasalvar, John M. Grigor, and Zulfiqur Ali 2.1 Introduction The practical evaluation of ...
The perspective on the development of nano and microsystems for chemical and biological measurements is presented. The use of nano and microfabrication approaches allows to create miniaturized analytical devices that are inherently... more
The perspective on the development of nano and microsystems for chemical and biological measurements is presented. The use of nano and microfabrication approaches allows to create miniaturized analytical devices that are inherently smaller, have lower power consumption, give lower ...
10 Analysis of Seafood Aroma/Odour by Electronic Nose Technology and Direct Analysis JOHN M. GRIGOR, BRENDEN J. THEAKER, CESARETTIN ALASALVAR, WILLIAM T. O'HARE, ZULFIQUR ALI Volatile aroma compounds are generally regarded to be... more
10 Analysis of Seafood Aroma/Odour by Electronic Nose Technology and Direct Analysis JOHN M. GRIGOR, BRENDEN J. THEAKER, CESARETTIN ALASALVAR, WILLIAM T. O'HARE, ZULFIQUR ALI Volatile aroma compounds are generally regarded to be important parameters for ...
An electronic nose based on an array of six coated Piezoelectric Quartz crystal (PZQ) sensors has been used for classification of fresh edible oils. The electronic nose was presented with 346 samples of fresh edible oil headspace... more
An electronic nose based on an array of six coated Piezoelectric Quartz crystal (PZQ) sensors has been used for classification of fresh edible oils. The electronic nose was presented with 346 samples of fresh edible oil headspace volatiles, generated at 45ºC. Extra virgin olive ...
Research Interests: Engineering, Technology, Fluid Mechanics, Microfluidics, Production, and 14 moreBiotechnology, Biological Sciences, Bioreactor, Bacteria, Pseudomonas aeruginosa, Applied Microbiology and Biotechnology, Anti-Bacterial Agents, Glycolipids, Miniaturization, Bioreactors, Surface Active Agents, Pseudomonas Aeruginosa, Equipment Design, and Microfluidic
Ali, Z. et. al. (2001) 'Application of a radial basis neural network for the classification of fresh edible oils using a piezoelectric sensor array based electronic nose', Systemics, cybernetics and informatics, in... more
Ali, Z. et. al. (2001) 'Application of a radial basis neural network for the classification of fresh edible oils using a piezoelectric sensor array based electronic nose', Systemics, cybernetics and informatics, in Cybernetics and informatics, III, pp.161-165.