BSc in Pharmacy/Biochemistry, MSc in Pharmacology/Biochemistry, Doctoral Candidate in Pathology/Experimental Pathology, Visiting Scholar at Tufts University/Biochemical and Molecular Nutrition.
Resveratrol (RSV), polyphenol from grape, was studied to evaluate its effects on calorimetric par... more Resveratrol (RSV), polyphenol from grape, was studied to evaluate its effects on calorimetric parameters, energy metabolism, and antioxidants in the myocardium of diabetic rats. The animals were randomly divided into four groups (n = 8): C (control group): normal rats; C-RSV: normal rats receiving RSV; DM: diabetic rats; and DM-RSV: diabetics rats receiving RSV. Type 1 diabetes mellitus was induced with administration of streptozotocin (STZ; 60 mg21 body weight, single dose, i.p.). After 48 hours of STZ administration, the animals received RSV (1.0 mg/kg/day) for gavage for 30 days. Food, water, and energy intake were higher in the DM group, while administration of RSV caused decreases (p,0.05) in these parameters. The glycemia decreased and higher final body weight increased in DM-RSV when compared with the DM group. The diabetic rats showed higher serum-free fatty acid, which was normalized with RSV. Oxygen consumption (VO2) and carbon dioxide production (VCO2) decreased (p,0.05) in the DM group. This was accompanied by reductions in RQ. The C-RSV group showed higher VO2 and VCO2 values. Pyruvate dehydrogenase activity was lower in the DM group and normalizes with RSV. The DM group exhibited higher myocardial b-hydroxyacyl coenzyme-A dehydrogenase and citrate synthase activity, and RSV decreased the activity of these enzymes. The DM group had higher cardiac lactate dehydrogenase compared to the DM-RSV group. Myocardial protein carbonyl was increased in the DM group. RSV increased reduced glutathione in the cardiac tissue of diabetic animals. The glutathione reductase activity was higher in the DM-RSV group compared to the DM group. In conclusion, diabetes is accompanied by cardiac energy metabolism dysfunction and change in the biomarkers of oxidative stress. The cardioprotective effect may be mediated through RVS’s ability to normalize free fatty acid oxidation, enhance utilization glucose, and control the biomarkers’ level of oxidative stress under diabetic conditions.
The effects of the inhalation of Cymbopogon martinii essential oil (EO) and geraniol onWistar rat... more The effects of the inhalation of Cymbopogon martinii essential oil (EO) and geraniol onWistar rats were evaluated for biochemical parameters and hepatic oxidative stress. Wistar rats were divided into three groups (𝑛 = 8): G1 was control group, treated with saline solution; G2 received geraniol; and G3 received C. martinii EO by inhalation during 30 days. No significant differences were observed in glycemia and triacylglycerol levels; G2 and G3 decreased (𝑃 < 0.05) total cholesterol level. There were no differences in serum protein, urea, aspartate aminotransferase activity, and total hepatic protein. Creatinine levels increased in G2 but decreased in G3. Alanine aminotransferase activity and lipid hydroperoxide were higher in G2 than in G3. Catalase and superoxide dismutase activities were higher in G3. C. martinii EO and geraniol increased glutathione peroxidase. Oxidative stress caused by geraniol may have triggered some degree of hepatic toxicity, as verified by the increase in serum creatinine and alanine aminotransferase. Therefore, the beneficial effects of EO on oxidative stress can prevent the toxicity in the liver. This proves possible interactions between geraniol and numerous chemical compounds present in C. martinii EO.
Resveratrol (RSV), polyphenol from grape, was studied to evaluate its effects on calorimetric par... more Resveratrol (RSV), polyphenol from grape, was studied to evaluate its effects on calorimetric parameters, energy metabolism, and antioxidants in the myocardium of diabetic rats. The animals were randomly divided into four groups (n = 8): C (control group): normal rats; C-RSV: normal rats receiving RSV; DM: diabetic rats; and DM-RSV: diabetics rats receiving RSV. Type 1 diabetes mellitus was induced with administration of streptozotocin (STZ; 60 mg21 body weight, single dose, i.p.). After 48 hours of STZ administration, the animals received RSV (1.0 mg/kg/day) for gavage for 30 days. Food, water, and energy intake were higher in the DM group, while administration of RSV caused decreases (p,0.05) in these parameters. The glycemia decreased and higher final body weight increased in DM-RSV when compared with the DM group. The diabetic rats showed higher serum-free fatty acid, which was normalized with RSV. Oxygen consumption (VO2) and carbon dioxide production (VCO2) decreased (p,0.05) in the DM group. This was accompanied by reductions in RQ. The C-RSV group showed higher VO2 and VCO2 values. Pyruvate dehydrogenase activity was lower in the DM group and normalizes with RSV. The DM group exhibited higher myocardial b-hydroxyacyl coenzyme-A dehydrogenase and citrate synthase activity, and RSV decreased the activity of these enzymes. The DM group had higher cardiac lactate dehydrogenase compared to the DM-RSV group. Myocardial protein carbonyl was increased in the DM group. RSV increased reduced glutathione in the cardiac tissue of diabetic animals. The glutathione reductase activity was higher in the DM-RSV group compared to the DM group. In conclusion, diabetes is accompanied by cardiac energy metabolism dysfunction and change in the biomarkers of oxidative stress. The cardioprotective effect may be mediated through RVS’s ability to normalize free fatty acid oxidation, enhance utilization glucose, and control the biomarkers’ level of oxidative stress under diabetic conditions.
The effects of the inhalation of Cymbopogon martinii essential oil (EO) and geraniol onWistar rat... more The effects of the inhalation of Cymbopogon martinii essential oil (EO) and geraniol onWistar rats were evaluated for biochemical parameters and hepatic oxidative stress. Wistar rats were divided into three groups (𝑛 = 8): G1 was control group, treated with saline solution; G2 received geraniol; and G3 received C. martinii EO by inhalation during 30 days. No significant differences were observed in glycemia and triacylglycerol levels; G2 and G3 decreased (𝑃 < 0.05) total cholesterol level. There were no differences in serum protein, urea, aspartate aminotransferase activity, and total hepatic protein. Creatinine levels increased in G2 but decreased in G3. Alanine aminotransferase activity and lipid hydroperoxide were higher in G2 than in G3. Catalase and superoxide dismutase activities were higher in G3. C. martinii EO and geraniol increased glutathione peroxidase. Oxidative stress caused by geraniol may have triggered some degree of hepatic toxicity, as verified by the increase in serum creatinine and alanine aminotransferase. Therefore, the beneficial effects of EO on oxidative stress can prevent the toxicity in the liver. This proves possible interactions between geraniol and numerous chemical compounds present in C. martinii EO.
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Papers by Klinsmann Carolo
metabolism, and antioxidants in the myocardium of diabetic rats. The animals were randomly divided into four groups
(n = 8): C (control group): normal rats; C-RSV: normal rats receiving RSV; DM: diabetic rats; and DM-RSV: diabetics rats
receiving RSV. Type 1 diabetes mellitus was induced with administration of streptozotocin (STZ; 60 mg21 body weight,
single dose, i.p.). After 48 hours of STZ administration, the animals received RSV (1.0 mg/kg/day) for gavage for 30 days.
Food, water, and energy intake were higher in the DM group, while administration of RSV caused decreases (p,0.05) in
these parameters. The glycemia decreased and higher final body weight increased in DM-RSV when compared with the DM
group. The diabetic rats showed higher serum-free fatty acid, which was normalized with RSV. Oxygen consumption (VO2)
and carbon dioxide production (VCO2) decreased (p,0.05) in the DM group. This was accompanied by reductions in RQ.
The C-RSV group showed higher VO2 and VCO2 values. Pyruvate dehydrogenase activity was lower in the DM group and
normalizes with RSV. The DM group exhibited higher myocardial b-hydroxyacyl coenzyme-A dehydrogenase and citrate
synthase activity, and RSV decreased the activity of these enzymes. The DM group had higher cardiac lactate
dehydrogenase compared to the DM-RSV group. Myocardial protein carbonyl was increased in the DM group. RSV increased
reduced glutathione in the cardiac tissue of diabetic animals. The glutathione reductase activity was higher in the DM-RSV
group compared to the DM group. In conclusion, diabetes is accompanied by cardiac energy metabolism dysfunction and
change in the biomarkers of oxidative stress. The cardioprotective effect may be mediated through RVS’s ability to
normalize free fatty acid oxidation, enhance utilization glucose, and control the biomarkers’ level of oxidative stress under
diabetic conditions.
parameters and hepatic oxidative stress. Wistar rats were divided into three groups (𝑛 = 8): G1 was control group, treated with
saline solution; G2 received geraniol; and G3 received C. martinii EO by inhalation during 30 days. No significant differences were
observed in glycemia and triacylglycerol levels; G2 and G3 decreased (𝑃 < 0.05) total cholesterol level. There were no differences in
serum protein, urea, aspartate aminotransferase activity, and total hepatic protein. Creatinine levels increased in G2 but decreased
in G3. Alanine aminotransferase activity and lipid hydroperoxide were higher in G2 than in G3. Catalase and superoxide dismutase
activities were higher in G3. C. martinii EO and geraniol increased glutathione peroxidase. Oxidative stress caused by geraniol
may have triggered some degree of hepatic toxicity, as verified by the increase in serum creatinine and alanine aminotransferase.
Therefore, the beneficial effects of EO on oxidative stress can prevent the toxicity in the liver. This proves possible interactions
between geraniol and numerous chemical compounds present in C. martinii EO.
metabolism, and antioxidants in the myocardium of diabetic rats. The animals were randomly divided into four groups
(n = 8): C (control group): normal rats; C-RSV: normal rats receiving RSV; DM: diabetic rats; and DM-RSV: diabetics rats
receiving RSV. Type 1 diabetes mellitus was induced with administration of streptozotocin (STZ; 60 mg21 body weight,
single dose, i.p.). After 48 hours of STZ administration, the animals received RSV (1.0 mg/kg/day) for gavage for 30 days.
Food, water, and energy intake were higher in the DM group, while administration of RSV caused decreases (p,0.05) in
these parameters. The glycemia decreased and higher final body weight increased in DM-RSV when compared with the DM
group. The diabetic rats showed higher serum-free fatty acid, which was normalized with RSV. Oxygen consumption (VO2)
and carbon dioxide production (VCO2) decreased (p,0.05) in the DM group. This was accompanied by reductions in RQ.
The C-RSV group showed higher VO2 and VCO2 values. Pyruvate dehydrogenase activity was lower in the DM group and
normalizes with RSV. The DM group exhibited higher myocardial b-hydroxyacyl coenzyme-A dehydrogenase and citrate
synthase activity, and RSV decreased the activity of these enzymes. The DM group had higher cardiac lactate
dehydrogenase compared to the DM-RSV group. Myocardial protein carbonyl was increased in the DM group. RSV increased
reduced glutathione in the cardiac tissue of diabetic animals. The glutathione reductase activity was higher in the DM-RSV
group compared to the DM group. In conclusion, diabetes is accompanied by cardiac energy metabolism dysfunction and
change in the biomarkers of oxidative stress. The cardioprotective effect may be mediated through RVS’s ability to
normalize free fatty acid oxidation, enhance utilization glucose, and control the biomarkers’ level of oxidative stress under
diabetic conditions.
parameters and hepatic oxidative stress. Wistar rats were divided into three groups (𝑛 = 8): G1 was control group, treated with
saline solution; G2 received geraniol; and G3 received C. martinii EO by inhalation during 30 days. No significant differences were
observed in glycemia and triacylglycerol levels; G2 and G3 decreased (𝑃 < 0.05) total cholesterol level. There were no differences in
serum protein, urea, aspartate aminotransferase activity, and total hepatic protein. Creatinine levels increased in G2 but decreased
in G3. Alanine aminotransferase activity and lipid hydroperoxide were higher in G2 than in G3. Catalase and superoxide dismutase
activities were higher in G3. C. martinii EO and geraniol increased glutathione peroxidase. Oxidative stress caused by geraniol
may have triggered some degree of hepatic toxicity, as verified by the increase in serum creatinine and alanine aminotransferase.
Therefore, the beneficial effects of EO on oxidative stress can prevent the toxicity in the liver. This proves possible interactions
between geraniol and numerous chemical compounds present in C. martinii EO.