The breeding of alpaca (Vicugna pacos) is one of the most important economic activities in the hi... more The breeding of alpaca (Vicugna pacos) is one of the most important economic activities in the high Andean areas of Peru. The commercialization of products derived from alpaca represents more than 80% of the income of high-Andean families. However, the infestation of parasites such as Sarcocystis lamacanis in the alpacas causes economic losses that deteriorate the already diminished quality of life of the alpaca breeder. The search for biomarkers that allow the early detection of these parasites is one of the most critical challenges in Peru, a country with the largest population of alpacas worldwide. This work aimed to analyze and quantify the microcysts formed by the parasite and relate them to the troponin cTnI level in the blood serum. Troponins are proteins secreted when there is damage to the cardiac muscle. 60 blood and cardiac tissue samples were collected from Tisco and La Raya slaughterhouses, localities of Caylloma Province in Arequipa, and Chucuito District in Puno, both regions in southern Peru. The cardiac muscle samples were processed with the routine histology technique and stained with hematoxylin and eosin. In addition, serum samples were processed with the ELISA and immunochromatography methods for troponin cTnI. Results were 100% positive for the presence of Sarcocystis lamacanis microcysts in all cardiac muscle samples. The average microcyst quantification per field of 100x were 3.5 and 5.7 for the Tisco and La Raya samples. In addition, several microscopic lesions were observed in the cardiac muscles: microcyst infiltration between muscle fibers, basophilic microcysts with a thick outer membrane and bradyzoites inside, and tissue displacement. On the other hand, all serum blood samples were negative for troponin cTnI, with both methods, ELISA and immunochromatography. For results, we infer troponin cTnI do not can be used as a biomarker for heart damage caused by Sarcocystis lamacanis parasite in alpacas.
This study evaluated sexual dimorphism and seasonal variations in corticotrophs and adrenal zona ... more This study evaluated sexual dimorphism and seasonal variations in corticotrophs and adrenal zona fasciculata in dogs, as well as the expression of oestrogen receptor alpha (ERα). An immunohistochemical analysis was conducted in pituitaries for ACTH and in adrenal glands for ERα and for the melanocortin-2-receptor (MC2R) in winter and summer. Double immunofluorescence was performed to identify ERα in corticotrophs. Females had a greater proportion of corticotrophs per field (p<0.01), with a greater cellular area and optical density (p<0.001) than males. Optical density of corticotrophs was greater in winter for both sexes (p<0.001). In zona fasciculata, ERα and MC2R expression was greater in females (p<0.001) and was greater in winter (p<0.001). ERα was identified in corticotrophs. This study is the first to demonstrate ERα expression in corticotrophs and the adrenal cortex in dogs, providing evidence for sexual dimorphism and seasonal variations.
Fil: Lorenzo, Maria Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Inst... more Fil: Lorenzo, Maria Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnologia en Reproduccion Animal; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Oocyte maturation in culture is still the weakest part of in vitro fertilization (IVF) and cocult... more Oocyte maturation in culture is still the weakest part of in vitro fertilization (IVF) and coculture with somatic cells may be an alternative to improve suboptimal culture conditions, especially in the pig in which maturation takes more than 44 h. In the present study, we investigated the effect of a coculture system of porcine luteal cells (PLC) during in vitro maturation (IVM) on embryo development and gene expression. Cumulus-oocyte complexes were matured in vitro in TCM-199 with human menopausal gonadotrophin (control) and in coculture with PLC. IVF was performed with frozen-thawed boar semen in Tris-buffered medium. Presumptive zygotes were cultured in PZM for 7 days. The coculture with PLC significantly increased blastocysts rates. Gene expression changes were measured with a porcine embryo-specific microarray and confirmed by RT-qPCR. The global transcription pattern of embryos developing after PLC coculture exhibited overall downregulation of gene expression. Following global gene expression pattern analysis, genes associated with lipid metabolism, mitochondrial function, endoplasmic reticulum stress, and apoptosis were found downregulated, and genes associated with cell cycle and proliferation were found upregulated in the PLC coculture. Canonical pathway analysis by Ingenuity Pathway revealed that differential expression transcripts were associated with the sirtuin signaling pathway, oxidative phosphorylation pathway, cytokines and ephrin receptor signaling. To conclude, the coculture system of PLC during IVM has a lasting effect on the embryo until the blastocyst stage, modifying gene expression, with a positive effect on embryo development. Our model could be an alternative to replace the conventional maturation medium with gonadotrophins with higher rates of embryo development, a key issue in porcine in vitro embryo production.
Nuclear Instruments & Methods in Physics Research Section B-beam Interactions With Materials and Atoms, Dec 1, 2001
Abstract Polymer surface modifications are obtained by the application of radiation treatments an... more Abstract Polymer surface modifications are obtained by the application of radiation treatments and other physico-chemical methods: fission fragment (ff) irradiation and etching. The biocompatibility of the surface is then observed by cell seeding and cell adhesion experiments. Approaches to improvement of the cell adhesion are obtained by different methods: for example, in PS, cell adhesion is improved after ion implantation; in PMMA, after bombarding the polymer, the surface is reconditioned with surfactants and proteins and in PVDF, cell adhesion is assayed on nuclear tracks membranes. In this work, we obtained important cell adhesion improvements in PP films by irradiation with swift heavy ions and subsequent etching of the nuclear tracks. We use BOPP (isotactic −25 μm thickness). Irrradiations were performed with a Cf-252 californium ff source. The source has a heavy ff and a light one, with 160–200 MeV energy divided among them corresponding to ff energies between 1 and 2 MeV/amu. A chemical etching procedure consisting of a solution of sulphuric acid and chromium three oxide at 85 °C was used. The 3T3 NIH fibroblast cell line was used for the cell adhesion experiment. Here we report for the first time, the results of a series of experiments by varying the ff fluence and the etching time showing that attachment and spreadout of cells are very much improved in this cell line according to the number of pores and the pore size.
Revista de investigaciones veterinarias del Perú, Apr 28, 2023
El conocimiento del desarrollo embrionario y fetal es de suma importancia para poder establecer p... more El conocimiento del desarrollo embrionario y fetal es de suma importancia para poder establecer parámetros de desarrollo normal, la salud del feto y para identificar periodos críticos en la curva de crecimiento. Uno de estos procesos es el patrón de osificación. El presente estudio describe el patrón de osificación en embriones y fetos de alpaca utilizando las técnicas de radiología y diafanización. Se utilizaron 17 alpacas hembra preñadas para la colección de embriones y fetos de variada edad (32, 45, 60, 90, 118, 150, 165, 190, 220 y 280 días). Los especímenes fueron pesados, medidos y radiografiados, y posteriormente fijados en formol tamponado al 10% para ser sometidos
Se han descrito áreas alopécicas en la zona lateral y medial del metatarso de los camélidos sudam... more Se han descrito áreas alopécicas en la zona lateral y medial del metatarso de los camélidos sudamericanos, y se les ha denominado “glándulas metatarsales”, análogas a las presentes en los cérvidos. En este estudio, se colectaron muestras de tejido de la región metatarsal de 8 especímenes (6 alpacas adultas y dos fetos [5 y 8 meses]) en el camal de Sicuani, Cusco (Perú), y fueron fijadas en formol tamponado al 10% y procesadas mediante la técnica histológica estándar. Seis muestras fueron teñidas con Hematoxilina-Eosina, PAS y Tricrómico de Golden Masson, y fueron evaluadas posteriormente mediante microscopía de campo claro, y dos muestras de alpacas adultas fueron procesadas mediante corte por congelación con un criostato y posterior tinción lipídica diferencial con rojo Nilo y contraste nuclear con Hoescht 33342, evaluándose mediante microscopio de fluorescencia. Se observó un tejido epidérmico alto, conformado por células principales del tipo lipoqueratinocitos, con presencia de v...
En las aves durante el desarrollo ovárico se pueden reconocer 4 eventos fundamentales: migración ... more En las aves durante el desarrollo ovárico se pueden reconocer 4 eventos fundamentales: migración y colonización de las células germinales primordiales, diferenciación y proliferación de ovogonias, organización de nidos germinales e inicio del proceso meiótico. El conocimiento de dichos eventos resulta fundamental para la interpretación de los procesos involucrados con la diferenciación de las gametas, sin embargo sólo existen referencias para algunas especies modelo como Gallus gallus domesticus y Coturnix coturnix. En el presente trabajo se describen los cambios y sucesos celulares implicados en la ontogenia ovárica. Los esbozos gonadales indiferenciados se observan, a partir del estadio 16, como dos masas alargadas dispuestas en el margen medio ventral del riñón. El conducto de Müller se distingue a partir del estadio 21, desde el extremo anterior del riñón y hasta la región de la cloaca. A partir del estadio 34 comienza a visualizarse la regresión de la gónada derecha y del corre...
La inseminación artificial intrauterina post-cervical (PCAI) en cerdas optimiza el rendimiento de... more La inseminación artificial intrauterina post-cervical (PCAI) en cerdas optimiza el rendimiento del cerdo macho respecto de la inseminación artificial estándar o cervical (CAI). Actualmente, la PCAI, sólo se utiliza en cerdas multíparas y no en cerdas nulíparas por lo que una demostración de utilidad de la PCAI en cerdas nulíparas podría aumentar el rendimiento de los establecimientos productivos. Con este objetivo se compararon las técnicas PCAI y CAI en dicha categoría de cerdas. Éste es un trabajo a pequeña escala realizado a campo, y representa un punto inicial para el empleo de esta técnica a nivel productivo. Se analizaron tasas de preñez post-inseminación (Pr), pérdidas de preñez (Perd) y número de crías nacidas vivas por cerda preñada (Crías). Se realizaron 4 CAI y 7 PCAI en cerdas nulíparas. No se observaron diferencias en Pr ni Perd (p>0,05); tampoco en Crías (p>0,05). Concluimos que la PCAI es una técnica válida en cerdas nulíparas.Fil: Luchetti, Carolina Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal. Cátedra de Histología y Embriología; ArgentinaFil: Renoulin, E. G.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal. Cátedra de Histología y Embriología; ArgentinaFil: Carou, María Clara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal. Cátedra de Histología y Embriología; ArgentinaFil: Lombardo, Daniel Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal. Cátedra de Histología y Embriología; Argentin
The oviduct is involved in many reproductive functions, including early embryo development. The e... more The oviduct is involved in many reproductive functions, including early embryo development. The epithelial cells that cover the oviduct produce oviducal fluid and could be used to recreate the invivo environment into which embryo development takes place. This study aimed to evaluate the co-culture of porcine embryos with a monolayer of porcine oviducal epithelial cells (POEC) and its effect on embryo development and quality. The POEC were obtained by pressing the isthmus (from diestrus sow oviducts) using slides and performing 3 cycles of vortexing and decanting in DMEM-F12 medium. Passage 1 cells were used for these experiments (POEC-1). Oocytes were obtained from follicular aspiration of slaughterhouse ovaries. Oocytes were invitro matured for 44h in TCM-199 supplemented with human menopausal gonadotrophin and cyclic AMP during the first 22h. Invitro fertilization was performed with 17°C-refrigerated boar semen for 4h in 100-µL drops of TCM-199 with caffeine, bovine serum albumin,...
l-Carnitine (LC) plays an important role in the catabolism of lipids and protects cells from the ... more l-Carnitine (LC) plays an important role in the catabolism of lipids and protects cells from the damage caused by reactive oxygen species due to its antioxidant activity. The aim of this study was to evaluate the effect of adding different concentrations of LC during porcine invitro maturation on embryo quality and development. The cumulus–oocyte complexes were obtained by follicular aspiration from ovaries of slaughtered sows and matured invitro for 44h without LC (control) or with different concentrations of LC (0.6 or 1.25mg mL−1) (Sigma-Aldrich) in TCM-199 supplemented with human menopausal gonadotrophin and cyclic AMP (cAMP) during the first 22h. Invitro fertilization was performed with fresh boar semen for 4h in 100-µL drops of TCM-199 with caffeine, bovine serum albumin, sodium lactate, and pyruvate (20 denuded oocytes per drop, 1×106 spermatozoa mL−1). Presumptive zygotes were washed and cultured in NCSU 23 at 39°C, 7% O2, 5% CO2, and humidity. The cleavage rate was register...
The breeding of alpaca (Vicugna pacos) is one of the most important economic activities in the hi... more The breeding of alpaca (Vicugna pacos) is one of the most important economic activities in the high Andean areas of Peru. The commercialization of products derived from alpaca represents more than 80% of the income of high-Andean families. However, the infestation of parasites such as Sarcocystis lamacanis in the alpacas causes economic losses that deteriorate the already diminished quality of life of the alpaca breeder. The search for biomarkers that allow the early detection of these parasites is one of the most critical challenges in Peru, a country with the largest population of alpacas worldwide. This work aimed to analyze and quantify the microcysts formed by the parasite and relate them to the troponin cTnI level in the blood serum. Troponins are proteins secreted when there is damage to the cardiac muscle. 60 blood and cardiac tissue samples were collected from Tisco and La Raya slaughterhouses, localities of Caylloma Province in Arequipa, and Chucuito District in Puno, both regions in southern Peru. The cardiac muscle samples were processed with the routine histology technique and stained with hematoxylin and eosin. In addition, serum samples were processed with the ELISA and immunochromatography methods for troponin cTnI. Results were 100% positive for the presence of Sarcocystis lamacanis microcysts in all cardiac muscle samples. The average microcyst quantification per field of 100x were 3.5 and 5.7 for the Tisco and La Raya samples. In addition, several microscopic lesions were observed in the cardiac muscles: microcyst infiltration between muscle fibers, basophilic microcysts with a thick outer membrane and bradyzoites inside, and tissue displacement. On the other hand, all serum blood samples were negative for troponin cTnI, with both methods, ELISA and immunochromatography. For results, we infer troponin cTnI do not can be used as a biomarker for heart damage caused by Sarcocystis lamacanis parasite in alpacas.
This study evaluated sexual dimorphism and seasonal variations in corticotrophs and adrenal zona ... more This study evaluated sexual dimorphism and seasonal variations in corticotrophs and adrenal zona fasciculata in dogs, as well as the expression of oestrogen receptor alpha (ERα). An immunohistochemical analysis was conducted in pituitaries for ACTH and in adrenal glands for ERα and for the melanocortin-2-receptor (MC2R) in winter and summer. Double immunofluorescence was performed to identify ERα in corticotrophs. Females had a greater proportion of corticotrophs per field (p<0.01), with a greater cellular area and optical density (p<0.001) than males. Optical density of corticotrophs was greater in winter for both sexes (p<0.001). In zona fasciculata, ERα and MC2R expression was greater in females (p<0.001) and was greater in winter (p<0.001). ERα was identified in corticotrophs. This study is the first to demonstrate ERα expression in corticotrophs and the adrenal cortex in dogs, providing evidence for sexual dimorphism and seasonal variations.
Fil: Lorenzo, Maria Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Inst... more Fil: Lorenzo, Maria Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnologia en Reproduccion Animal; Argentina. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Oocyte maturation in culture is still the weakest part of in vitro fertilization (IVF) and cocult... more Oocyte maturation in culture is still the weakest part of in vitro fertilization (IVF) and coculture with somatic cells may be an alternative to improve suboptimal culture conditions, especially in the pig in which maturation takes more than 44 h. In the present study, we investigated the effect of a coculture system of porcine luteal cells (PLC) during in vitro maturation (IVM) on embryo development and gene expression. Cumulus-oocyte complexes were matured in vitro in TCM-199 with human menopausal gonadotrophin (control) and in coculture with PLC. IVF was performed with frozen-thawed boar semen in Tris-buffered medium. Presumptive zygotes were cultured in PZM for 7 days. The coculture with PLC significantly increased blastocysts rates. Gene expression changes were measured with a porcine embryo-specific microarray and confirmed by RT-qPCR. The global transcription pattern of embryos developing after PLC coculture exhibited overall downregulation of gene expression. Following global gene expression pattern analysis, genes associated with lipid metabolism, mitochondrial function, endoplasmic reticulum stress, and apoptosis were found downregulated, and genes associated with cell cycle and proliferation were found upregulated in the PLC coculture. Canonical pathway analysis by Ingenuity Pathway revealed that differential expression transcripts were associated with the sirtuin signaling pathway, oxidative phosphorylation pathway, cytokines and ephrin receptor signaling. To conclude, the coculture system of PLC during IVM has a lasting effect on the embryo until the blastocyst stage, modifying gene expression, with a positive effect on embryo development. Our model could be an alternative to replace the conventional maturation medium with gonadotrophins with higher rates of embryo development, a key issue in porcine in vitro embryo production.
Nuclear Instruments & Methods in Physics Research Section B-beam Interactions With Materials and Atoms, Dec 1, 2001
Abstract Polymer surface modifications are obtained by the application of radiation treatments an... more Abstract Polymer surface modifications are obtained by the application of radiation treatments and other physico-chemical methods: fission fragment (ff) irradiation and etching. The biocompatibility of the surface is then observed by cell seeding and cell adhesion experiments. Approaches to improvement of the cell adhesion are obtained by different methods: for example, in PS, cell adhesion is improved after ion implantation; in PMMA, after bombarding the polymer, the surface is reconditioned with surfactants and proteins and in PVDF, cell adhesion is assayed on nuclear tracks membranes. In this work, we obtained important cell adhesion improvements in PP films by irradiation with swift heavy ions and subsequent etching of the nuclear tracks. We use BOPP (isotactic −25 μm thickness). Irrradiations were performed with a Cf-252 californium ff source. The source has a heavy ff and a light one, with 160–200 MeV energy divided among them corresponding to ff energies between 1 and 2 MeV/amu. A chemical etching procedure consisting of a solution of sulphuric acid and chromium three oxide at 85 °C was used. The 3T3 NIH fibroblast cell line was used for the cell adhesion experiment. Here we report for the first time, the results of a series of experiments by varying the ff fluence and the etching time showing that attachment and spreadout of cells are very much improved in this cell line according to the number of pores and the pore size.
Revista de investigaciones veterinarias del Perú, Apr 28, 2023
El conocimiento del desarrollo embrionario y fetal es de suma importancia para poder establecer p... more El conocimiento del desarrollo embrionario y fetal es de suma importancia para poder establecer parámetros de desarrollo normal, la salud del feto y para identificar periodos críticos en la curva de crecimiento. Uno de estos procesos es el patrón de osificación. El presente estudio describe el patrón de osificación en embriones y fetos de alpaca utilizando las técnicas de radiología y diafanización. Se utilizaron 17 alpacas hembra preñadas para la colección de embriones y fetos de variada edad (32, 45, 60, 90, 118, 150, 165, 190, 220 y 280 días). Los especímenes fueron pesados, medidos y radiografiados, y posteriormente fijados en formol tamponado al 10% para ser sometidos
Se han descrito áreas alopécicas en la zona lateral y medial del metatarso de los camélidos sudam... more Se han descrito áreas alopécicas en la zona lateral y medial del metatarso de los camélidos sudamericanos, y se les ha denominado “glándulas metatarsales”, análogas a las presentes en los cérvidos. En este estudio, se colectaron muestras de tejido de la región metatarsal de 8 especímenes (6 alpacas adultas y dos fetos [5 y 8 meses]) en el camal de Sicuani, Cusco (Perú), y fueron fijadas en formol tamponado al 10% y procesadas mediante la técnica histológica estándar. Seis muestras fueron teñidas con Hematoxilina-Eosina, PAS y Tricrómico de Golden Masson, y fueron evaluadas posteriormente mediante microscopía de campo claro, y dos muestras de alpacas adultas fueron procesadas mediante corte por congelación con un criostato y posterior tinción lipídica diferencial con rojo Nilo y contraste nuclear con Hoescht 33342, evaluándose mediante microscopio de fluorescencia. Se observó un tejido epidérmico alto, conformado por células principales del tipo lipoqueratinocitos, con presencia de v...
En las aves durante el desarrollo ovárico se pueden reconocer 4 eventos fundamentales: migración ... more En las aves durante el desarrollo ovárico se pueden reconocer 4 eventos fundamentales: migración y colonización de las células germinales primordiales, diferenciación y proliferación de ovogonias, organización de nidos germinales e inicio del proceso meiótico. El conocimiento de dichos eventos resulta fundamental para la interpretación de los procesos involucrados con la diferenciación de las gametas, sin embargo sólo existen referencias para algunas especies modelo como Gallus gallus domesticus y Coturnix coturnix. En el presente trabajo se describen los cambios y sucesos celulares implicados en la ontogenia ovárica. Los esbozos gonadales indiferenciados se observan, a partir del estadio 16, como dos masas alargadas dispuestas en el margen medio ventral del riñón. El conducto de Müller se distingue a partir del estadio 21, desde el extremo anterior del riñón y hasta la región de la cloaca. A partir del estadio 34 comienza a visualizarse la regresión de la gónada derecha y del corre...
La inseminación artificial intrauterina post-cervical (PCAI) en cerdas optimiza el rendimiento de... more La inseminación artificial intrauterina post-cervical (PCAI) en cerdas optimiza el rendimiento del cerdo macho respecto de la inseminación artificial estándar o cervical (CAI). Actualmente, la PCAI, sólo se utiliza en cerdas multíparas y no en cerdas nulíparas por lo que una demostración de utilidad de la PCAI en cerdas nulíparas podría aumentar el rendimiento de los establecimientos productivos. Con este objetivo se compararon las técnicas PCAI y CAI en dicha categoría de cerdas. Éste es un trabajo a pequeña escala realizado a campo, y representa un punto inicial para el empleo de esta técnica a nivel productivo. Se analizaron tasas de preñez post-inseminación (Pr), pérdidas de preñez (Perd) y número de crías nacidas vivas por cerda preñada (Crías). Se realizaron 4 CAI y 7 PCAI en cerdas nulíparas. No se observaron diferencias en Pr ni Perd (p>0,05); tampoco en Crías (p>0,05). Concluimos que la PCAI es una técnica válida en cerdas nulíparas.Fil: Luchetti, Carolina Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal. Cátedra de Histología y Embriología; ArgentinaFil: Renoulin, E. G.. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal. Cátedra de Histología y Embriología; ArgentinaFil: Carou, María Clara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal. Cátedra de Histología y Embriología; ArgentinaFil: Lombardo, Daniel Marcelo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigación y Tecnología en Reproducción Animal. Cátedra de Histología y Embriología; Argentin
The oviduct is involved in many reproductive functions, including early embryo development. The e... more The oviduct is involved in many reproductive functions, including early embryo development. The epithelial cells that cover the oviduct produce oviducal fluid and could be used to recreate the invivo environment into which embryo development takes place. This study aimed to evaluate the co-culture of porcine embryos with a monolayer of porcine oviducal epithelial cells (POEC) and its effect on embryo development and quality. The POEC were obtained by pressing the isthmus (from diestrus sow oviducts) using slides and performing 3 cycles of vortexing and decanting in DMEM-F12 medium. Passage 1 cells were used for these experiments (POEC-1). Oocytes were obtained from follicular aspiration of slaughterhouse ovaries. Oocytes were invitro matured for 44h in TCM-199 supplemented with human menopausal gonadotrophin and cyclic AMP during the first 22h. Invitro fertilization was performed with 17°C-refrigerated boar semen for 4h in 100-µL drops of TCM-199 with caffeine, bovine serum albumin,...
l-Carnitine (LC) plays an important role in the catabolism of lipids and protects cells from the ... more l-Carnitine (LC) plays an important role in the catabolism of lipids and protects cells from the damage caused by reactive oxygen species due to its antioxidant activity. The aim of this study was to evaluate the effect of adding different concentrations of LC during porcine invitro maturation on embryo quality and development. The cumulus–oocyte complexes were obtained by follicular aspiration from ovaries of slaughtered sows and matured invitro for 44h without LC (control) or with different concentrations of LC (0.6 or 1.25mg mL−1) (Sigma-Aldrich) in TCM-199 supplemented with human menopausal gonadotrophin and cyclic AMP (cAMP) during the first 22h. Invitro fertilization was performed with fresh boar semen for 4h in 100-µL drops of TCM-199 with caffeine, bovine serum albumin, sodium lactate, and pyruvate (20 denuded oocytes per drop, 1×106 spermatozoa mL−1). Presumptive zygotes were washed and cultured in NCSU 23 at 39°C, 7% O2, 5% CO2, and humidity. The cleavage rate was register...
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