Experienced Postdoctoral Researcher with a demonstrated history of working in the research industry. Skilled in Molecular Biology, Plant Propagation including Tissue Culture, and Plant Phenotyping. Strong research professional with a Doctor of Agricultural Sciences focused in Horticulture from Warsaw University of Life Sciences.
Rooting is an important step in vegetative plant propagation; failure to form roots always create... more Rooting is an important step in vegetative plant propagation; failure to form roots always creates losses. EU-imposed restrictions on chemicals used in plant production create need for test of new natural products that may enhance rooting in commercial production. This study tested the effect of two biological preparations: AlgaminoPlant and Route on the difficult-to-root smokebush in conventional propagation and in micropropagation. Stem cuttings were sprayed with the biostimulators in different combinations and their efficiency was compared to the commonly used synthetic auxin β-indolyle butyric acid (IBA) used either as a commercial rooting powder Rhizopon AA or a foliar application of the aqueous solution of IBA. The success rate was measured by the rooting percentage and the degree of root ball development. Treatment with a aqueaous solution of IBA was more effective than Rhizopon AA. AlgaminoPlant and Route enhanced rhizogenesis so they are viable substitutes for synthetic auxins in conventional propagation of C. coggygria. In in vitro propagation, microshoots were cultured on Murashige and Skoog (MS) medium supplemented with IBA or biostimulators. The highest rooting rate was recorded for the 10-30 mg dm-3 IBA. AlgaminoPlant added to the medium increased the number of roots produced by microcuttings while Route had a deleterious effect. The experiments show that bio-stimulators are commercially viable substitutes for commonly used hormones in nursery handling of even difficult stocks.
Effect of biopreparations Amino Total and Biochikol 020 PC on rhizogenesis in stem cuttings of Ph... more Effect of biopreparations Amino Total and Biochikol 020 PC on rhizogenesis in stem cuttings of Physocarpus opulifolius 'Dart's Gold' and 'Diabolo'. The experiments were carried out on Physocarpus opulifolius 'Dart's Gold' and 'Diabolo'in the nursery of M.M. Kryt in Wola Prażmowska near Warsaw, in 2008. Stem cuttings were treated with the commercial rooting powders Ukorzeniacz AB or Rhizopon AA and/or sprayed with biostimulators Amino Total (0.1%) and Biochikol 020 PC (2%) in different combinations. A percentage of rooted cuttings was determined and a degree of the root ball development was evaluated after 6 weeks. Rhizopon AA containing IBA was more suitable for rooting the cuttings of Physocarpus opuli-folius cultivars than Ukorzeniacz AB containing NAA and in both cultivars it enhanced the rhizogenesis regardless the sprayings with bio-preparations. Amino Total and Biochikol 020 PC positively affected the rhizogenesis so they can be regarded as substitutes for synthetic auxins in stimulating the rooting of cuttings in Physocar-pus opulifolius. Both biopreparations increased the contents of chlorophyll a+b, free amino acids and polyphenolic acids while decreased the levels of reducing sugars and sucrose in cuttings during their rooting.
The UE-imposed restrictions on the manufacture and application of plant protection chemicals impo... more The UE-imposed restrictions on the manufacture and application of plant protection chemicals impose on the nurseryman the need to screen for new substances that are environmentally friendly and yet effective in the production of plant material. Biopre-parations may constitute such a group as they contain substance little affecting the environment. The aim of the work was to evaluate effects of the biopreparation Algamino-Plant on rooting of stem cuttings in Cornus alba 'Aurea' and C. alba 'Elegantissima'. Freshly harvested cuttings were sprayed with the 0.2% water solution of the bioprepara-tion. To compare its effectiveness with the commercial rooting powders used routinely in the nursery production part of the cuttings were treated with " Ukorzeniacz AB " (0.3% NAA) or Rhizopon AA (2% IBA). Both powders little affected rhizogenesis. Application of the biopreparation increased percentage and degree of rooting in dogwood as compared to control cuttings what shows its potential in a commercial production. The above treatment increased chlorophyll, carbohydrate contents and free amino acids in leaves of the sprayed cuttings.
The smoke bush (Cotinus coggygria Scop.) is a popular and highly demanded ornamental shrub whose ... more The smoke bush (Cotinus coggygria Scop.) is a popular and highly demanded ornamental shrub whose propagation can be problematic. The aim of the study was to test the effect of the mineral salt composition in the medium, concentration of plant growth regulators and the medium pH on the in vitro multiplication and shoot growth of C. cog-gygria Scop. 'Royal Purple'. Shoot tips were cultured on the following media: Anderson (AN), Murashige and Skoog (MS) (full-, half-and quarter strength), Nitsch and Nitsch (NN), Quoirin and Lepoivre (QL) or Lloyd and McCown (WPM). Media were supplemented with 6-benzyladenine (BA) in combinations with 1-naphthaleneacetic acid (NAA). The medium type and a concentration of the cytokinin significantly affected the regeneration rate of explants, the number, length and weight of axillary shoots as well as callus growth. The MS medium containing full strength of mineral salts with pH 5.8 was the best. High shoot proliferation (100%) and the highest number of shoots per explant (4.6) were obtained due to the use of 1.0 mg·dm-3 BA in combination with 0.1 mg·dm-3 NAA .
Cornus alba L. (white dogwood) is an important ornamental shrub having a wide range of applicatio... more Cornus alba L. (white dogwood) is an important ornamental shrub having a wide range of applications such as reforestation programs and soil retention systems. The vegeta-tive propagation of dogwood by cuttings may be slow, difficult, and cultivar dependent; therefore, an improved micropropagation method was developed. Nodal stem segments of C. alba cultivars 'Aurea' and 'Elegantissima' were cultured on media enriched with six different sources of mac-ronutrients. Media were supplemented with either N 6-benzyladenine (BA) or thidiazuron (TDZ) in combination with 1-naphthaleneacetic acid (NAA). Regardless of the cultivar, the best shoot proliferation was observed on Lloyd and McCown medium (woody plant medium (WPM)) at pH 6.2, containing 1.0 mg L −1 BA, 0.1 mg L −1 NAA, and 20–30 g L −1 sucrose. Rooting of regenerated shoots was achieved by an in vitro method when different concentrations of NAA or indole-3-butyric acid (IBA) were tested. Microcuttings were rooted for 8 wk on medium enriched with 0.25 mg L −1 NAA and potted into P9 containers in the greenhouse. The final survival rate of the plants after 20 wk was 80% for 'Aurea' and 90% for 'Elegantissima'. Genetic stability of the micropropagated plants was confirmed by using two DNA-based molecular marker techniques. A total of 30 random amplified polymor-phic DNA (RAPD) and 20 inter-simple sequence repeat (ISSR) primers resulted in 197–199 and 184–187 distinct and reproducible band classes, respectively, in 'Aurea' and 'Elegantissima' plantlets. All of the RAPD and ISSR profiles were monomorphic and comparable with the mother plant.
Efficient plant micropropagation depends upon a number of factors one of which is the type and co... more Efficient plant micropropagation depends upon a number of factors one of which is the type and concentration of exogenously supplied carbon sources in the medium. This study tested several different sugars as carbon source on the efficiency of shoot proliferation and in vitro rooting of common ninebark (Physocarpus opulifolius (L.) Maxim.). Fructose, glucose, maltose and sucrose were tested at concentration ranging from 0–50 g dm-3. The dry matter content, reducing sugars and sucrose in shoots were related to sugar concentrations in the medium and so was the rate of adventitious root formation. Sucrose did not stimulate shoot proliferation and glucose was completely ineffective in rooting induction. The highest number of shoots was produced on the fructose-containing medium. The concentration of 30 g dm-3 appeared to be optimal; the rate of proliferation at 30 and 40 g dm-3 were in fact similar, but the former produced a more favorable shoot length. The number of adventitious roots produced per shoot increased with increasing fructose concentration up to 30 g dm-3. Fructose can be therefore recommended as the best C-source for the in vitro shoot proliferation and rooting in common ninebark.
Induction of adventitious roots is crucial for the in vitro micropropagation of woody species. In... more Induction of adventitious roots is crucial for the in vitro micropropagation of woody species. In this study the histological and biochemical aspects of the adventitious root formation in microcuttings of smoke tree were evaluated. Smoke tree shoots grown on Murashige and Skoog's (1962) medium with 1 mg dm −3 N 6-benzyladenine (BA) and 0.1 mg dm −3 1-naphtaleneacetic acid (NAA) were transferred onto a rooting medium with or without 20 mg dm −3 indole-3-butyric acid (IBA) and sampled at various intervals for histological and biochemical analyses of the rooting process. First cell divisions were observed on the first day of culture in the area of primary bark identifiable root primordia with an oval shape and several cell layers were present at Day 3 in cuttings on both media. Roots with organized tissue were observed 9 days after the root induction on the medium with IBA; In shoots rooted on medium with IBA, the induction phase corresponded to a transient increase in the oxidase indole-3-acetic acid (IAAO) activity and levels of endogenous indole-3-acetic acid (IAA) and hydrogen peroxide (H 2 O 2) coincidental with a reduction in the peroxidase (POX) and polyphenol oxidase (PPO) activity and the polyphenoloacids contents. An increase the PPO and POX activity, accompanied by a low activity of IAAO during phases of root development and elongation resulted in a reduction of the endogenous auxin and H 2 O 2 contents in the IBA-treated cuttings.
Rooting is an important step in vegetative plant propagation; failure to form roots always create... more Rooting is an important step in vegetative plant propagation; failure to form roots always creates losses. EU-imposed restrictions on chemicals used in plant production create need for test of new natural products that may enhance rooting in commercial production. This study tested the effect of two biological preparations: AlgaminoPlant and Route on the difficult-to-root smokebush in conventional propagation and in micropropagation. Stem cuttings were sprayed with the biostimulators in different combinations and their efficiency was compared to the commonly used synthetic auxin β-indolyle butyric acid (IBA) used either as a commercial rooting powder Rhizopon AA or a foliar application of the aqueous solution of IBA. The success rate was measured by the rooting percentage and the degree of root ball development. Treatment with a aqueaous solution of IBA was more effective than Rhizopon AA. AlgaminoPlant and Route enhanced rhizogenesis so they are viable substitutes for synthetic auxins in conventional propagation of C. coggygria. In in vitro propagation, microshoots were cultured on Murashige and Skoog (MS) medium supplemented with IBA or biostimulators. The highest rooting rate was recorded for the 10-30 mg dm-3 IBA. AlgaminoPlant added to the medium increased the number of roots produced by microcuttings while Route had a deleterious effect. The experiments show that bio-stimulators are commercially viable substitutes for commonly used hormones in nursery handling of even difficult stocks.
Effect of biopreparations Amino Total and Biochikol 020 PC on rhizogenesis in stem cuttings of Ph... more Effect of biopreparations Amino Total and Biochikol 020 PC on rhizogenesis in stem cuttings of Physocarpus opulifolius 'Dart's Gold' and 'Diabolo'. The experiments were carried out on Physocarpus opulifolius 'Dart's Gold' and 'Diabolo'in the nursery of M.M. Kryt in Wola Prażmowska near Warsaw, in 2008. Stem cuttings were treated with the commercial rooting powders Ukorzeniacz AB or Rhizopon AA and/or sprayed with biostimulators Amino Total (0.1%) and Biochikol 020 PC (2%) in different combinations. A percentage of rooted cuttings was determined and a degree of the root ball development was evaluated after 6 weeks. Rhizopon AA containing IBA was more suitable for rooting the cuttings of Physocarpus opuli-folius cultivars than Ukorzeniacz AB containing NAA and in both cultivars it enhanced the rhizogenesis regardless the sprayings with bio-preparations. Amino Total and Biochikol 020 PC positively affected the rhizogenesis so they can be regarded as substitutes for synthetic auxins in stimulating the rooting of cuttings in Physocar-pus opulifolius. Both biopreparations increased the contents of chlorophyll a+b, free amino acids and polyphenolic acids while decreased the levels of reducing sugars and sucrose in cuttings during their rooting.
The UE-imposed restrictions on the manufacture and application of plant protection chemicals impo... more The UE-imposed restrictions on the manufacture and application of plant protection chemicals impose on the nurseryman the need to screen for new substances that are environmentally friendly and yet effective in the production of plant material. Biopre-parations may constitute such a group as they contain substance little affecting the environment. The aim of the work was to evaluate effects of the biopreparation Algamino-Plant on rooting of stem cuttings in Cornus alba 'Aurea' and C. alba 'Elegantissima'. Freshly harvested cuttings were sprayed with the 0.2% water solution of the bioprepara-tion. To compare its effectiveness with the commercial rooting powders used routinely in the nursery production part of the cuttings were treated with " Ukorzeniacz AB " (0.3% NAA) or Rhizopon AA (2% IBA). Both powders little affected rhizogenesis. Application of the biopreparation increased percentage and degree of rooting in dogwood as compared to control cuttings what shows its potential in a commercial production. The above treatment increased chlorophyll, carbohydrate contents and free amino acids in leaves of the sprayed cuttings.
The smoke bush (Cotinus coggygria Scop.) is a popular and highly demanded ornamental shrub whose ... more The smoke bush (Cotinus coggygria Scop.) is a popular and highly demanded ornamental shrub whose propagation can be problematic. The aim of the study was to test the effect of the mineral salt composition in the medium, concentration of plant growth regulators and the medium pH on the in vitro multiplication and shoot growth of C. cog-gygria Scop. 'Royal Purple'. Shoot tips were cultured on the following media: Anderson (AN), Murashige and Skoog (MS) (full-, half-and quarter strength), Nitsch and Nitsch (NN), Quoirin and Lepoivre (QL) or Lloyd and McCown (WPM). Media were supplemented with 6-benzyladenine (BA) in combinations with 1-naphthaleneacetic acid (NAA). The medium type and a concentration of the cytokinin significantly affected the regeneration rate of explants, the number, length and weight of axillary shoots as well as callus growth. The MS medium containing full strength of mineral salts with pH 5.8 was the best. High shoot proliferation (100%) and the highest number of shoots per explant (4.6) were obtained due to the use of 1.0 mg·dm-3 BA in combination with 0.1 mg·dm-3 NAA .
Cornus alba L. (white dogwood) is an important ornamental shrub having a wide range of applicatio... more Cornus alba L. (white dogwood) is an important ornamental shrub having a wide range of applications such as reforestation programs and soil retention systems. The vegeta-tive propagation of dogwood by cuttings may be slow, difficult, and cultivar dependent; therefore, an improved micropropagation method was developed. Nodal stem segments of C. alba cultivars 'Aurea' and 'Elegantissima' were cultured on media enriched with six different sources of mac-ronutrients. Media were supplemented with either N 6-benzyladenine (BA) or thidiazuron (TDZ) in combination with 1-naphthaleneacetic acid (NAA). Regardless of the cultivar, the best shoot proliferation was observed on Lloyd and McCown medium (woody plant medium (WPM)) at pH 6.2, containing 1.0 mg L −1 BA, 0.1 mg L −1 NAA, and 20–30 g L −1 sucrose. Rooting of regenerated shoots was achieved by an in vitro method when different concentrations of NAA or indole-3-butyric acid (IBA) were tested. Microcuttings were rooted for 8 wk on medium enriched with 0.25 mg L −1 NAA and potted into P9 containers in the greenhouse. The final survival rate of the plants after 20 wk was 80% for 'Aurea' and 90% for 'Elegantissima'. Genetic stability of the micropropagated plants was confirmed by using two DNA-based molecular marker techniques. A total of 30 random amplified polymor-phic DNA (RAPD) and 20 inter-simple sequence repeat (ISSR) primers resulted in 197–199 and 184–187 distinct and reproducible band classes, respectively, in 'Aurea' and 'Elegantissima' plantlets. All of the RAPD and ISSR profiles were monomorphic and comparable with the mother plant.
Efficient plant micropropagation depends upon a number of factors one of which is the type and co... more Efficient plant micropropagation depends upon a number of factors one of which is the type and concentration of exogenously supplied carbon sources in the medium. This study tested several different sugars as carbon source on the efficiency of shoot proliferation and in vitro rooting of common ninebark (Physocarpus opulifolius (L.) Maxim.). Fructose, glucose, maltose and sucrose were tested at concentration ranging from 0–50 g dm-3. The dry matter content, reducing sugars and sucrose in shoots were related to sugar concentrations in the medium and so was the rate of adventitious root formation. Sucrose did not stimulate shoot proliferation and glucose was completely ineffective in rooting induction. The highest number of shoots was produced on the fructose-containing medium. The concentration of 30 g dm-3 appeared to be optimal; the rate of proliferation at 30 and 40 g dm-3 were in fact similar, but the former produced a more favorable shoot length. The number of adventitious roots produced per shoot increased with increasing fructose concentration up to 30 g dm-3. Fructose can be therefore recommended as the best C-source for the in vitro shoot proliferation and rooting in common ninebark.
Induction of adventitious roots is crucial for the in vitro micropropagation of woody species. In... more Induction of adventitious roots is crucial for the in vitro micropropagation of woody species. In this study the histological and biochemical aspects of the adventitious root formation in microcuttings of smoke tree were evaluated. Smoke tree shoots grown on Murashige and Skoog's (1962) medium with 1 mg dm −3 N 6-benzyladenine (BA) and 0.1 mg dm −3 1-naphtaleneacetic acid (NAA) were transferred onto a rooting medium with or without 20 mg dm −3 indole-3-butyric acid (IBA) and sampled at various intervals for histological and biochemical analyses of the rooting process. First cell divisions were observed on the first day of culture in the area of primary bark identifiable root primordia with an oval shape and several cell layers were present at Day 3 in cuttings on both media. Roots with organized tissue were observed 9 days after the root induction on the medium with IBA; In shoots rooted on medium with IBA, the induction phase corresponded to a transient increase in the oxidase indole-3-acetic acid (IAAO) activity and levels of endogenous indole-3-acetic acid (IAA) and hydrogen peroxide (H 2 O 2) coincidental with a reduction in the peroxidase (POX) and polyphenol oxidase (PPO) activity and the polyphenoloacids contents. An increase the PPO and POX activity, accompanied by a low activity of IAAO during phases of root development and elongation resulted in a reduction of the endogenous auxin and H 2 O 2 contents in the IBA-treated cuttings.
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