This article, a collaboration between a literary scholar and a scientist, examines Livingstone&am... more This article, a collaboration between a literary scholar and a scientist, examines Livingstone's poetry to see how his scientific views affected his later poetry and, in particular, a mystical sense of nature. We suggest that Livingstone moves from the earlier idealism of the scientist as hunter and destroyer of evil or the even earlier sense of man as male‐master of
The bulk of this book describes the viruses and virus taxa approved by the International Committe... more The bulk of this book describes the viruses and virus taxa approved by the International Committee on Virus Taxonomy (ICTV) up to 1999, incorporating, in addition to the contents of the Sixth Report, decisions made at the Tenth International Congress of Virology in 1996 and at ...
Mol. Plant Pathol.(published by the British Society for …
The investigation of genetic diversity among maize, grass and wheat isolates of maize streak viru... more The investigation of genetic diversity among maize, grass and wheat isolates of maize streak virus (MSV) and other Mastreviruses (family: Geminiviridae) is described. The products of a degenerate primer polymerase chain reaction ...
Chlorotic, streak-like symptoms were observed in April 2013 on a single specimen of Albuca rautan... more Chlorotic, streak-like symptoms were observed in April 2013 on a single specimen of Albuca rautanenii (Schinz) J.C.Manning & Goldblatt (Family: Hyacinthaceae) found among other plants near Homeb in the Namib Desert, Namibia. No potential insect vectors (e.g., aphids) were observed on or around the infected plant. An extract from symptomatic leaves was assessed by transmission electron microscopy (leaf dip method) to ascertain if the symptoms were viral in origin. Long, flexuous threadlike particles 687 to 825 nm in length and 12.5 nm in diameter were observed. The morphology and size of the particles were indicative of a putative member of the taxonomic family Potyviridae. To confirm this, RT-PCR using universal potyvirus primers which amplify part of the nuclear inclusion b gene (NIb) was conducted (1) on total RNA extracted from leaf tissue (Qiagen RNeasy Plant Mini Kit). The triplicated reaction yielded amplicons of the expected size (~350 bp), which were cloned into the pJET 1.2...
The feasibility of applying molecular phylogenetic methods of analysis to aligned coat-protein se... more The feasibility of applying molecular phylogenetic methods of analysis to aligned coat-protein sequences and other molecular data derived from coat proteins or genomic sequences of members of the proposed taxonomic family of Potyviridae, is discussed. We show that comparative sequence analysis of whole coat-protein sequences may be used reliably to differentiate between sequences of closely related strains, and to show groupings of more distantly related viruses; that coat proteins of putative Potyviridae cluster according to the proposed generic divisions, and, even if some are only very distantly related, the members of the family form a cluster distinct from coat proteins of other filamentous and rod-shaped viruses. Taxonomic revisions based on perceived evolutionary relationships, and the lack of feasibility of erecting higher taxa for these viruses, are discussed.
This article, a collaboration between a literary scholar and a scientist, examines Livingstone&am... more This article, a collaboration between a literary scholar and a scientist, examines Livingstone's poetry to see how his scientific views affected his later poetry and, in particular, a mystical sense of nature. We suggest that Livingstone moves from the earlier idealism of the scientist as hunter and destroyer of evil or the even earlier sense of man as male‐master of
The bulk of this book describes the viruses and virus taxa approved by the International Committe... more The bulk of this book describes the viruses and virus taxa approved by the International Committee on Virus Taxonomy (ICTV) up to 1999, incorporating, in addition to the contents of the Sixth Report, decisions made at the Tenth International Congress of Virology in 1996 and at ...
Mol. Plant Pathol.(published by the British Society for …
The investigation of genetic diversity among maize, grass and wheat isolates of maize streak viru... more The investigation of genetic diversity among maize, grass and wheat isolates of maize streak virus (MSV) and other Mastreviruses (family: Geminiviridae) is described. The products of a degenerate primer polymerase chain reaction ...
Chlorotic, streak-like symptoms were observed in April 2013 on a single specimen of Albuca rautan... more Chlorotic, streak-like symptoms were observed in April 2013 on a single specimen of Albuca rautanenii (Schinz) J.C.Manning & Goldblatt (Family: Hyacinthaceae) found among other plants near Homeb in the Namib Desert, Namibia. No potential insect vectors (e.g., aphids) were observed on or around the infected plant. An extract from symptomatic leaves was assessed by transmission electron microscopy (leaf dip method) to ascertain if the symptoms were viral in origin. Long, flexuous threadlike particles 687 to 825 nm in length and 12.5 nm in diameter were observed. The morphology and size of the particles were indicative of a putative member of the taxonomic family Potyviridae. To confirm this, RT-PCR using universal potyvirus primers which amplify part of the nuclear inclusion b gene (NIb) was conducted (1) on total RNA extracted from leaf tissue (Qiagen RNeasy Plant Mini Kit). The triplicated reaction yielded amplicons of the expected size (~350 bp), which were cloned into the pJET 1.2...
The feasibility of applying molecular phylogenetic methods of analysis to aligned coat-protein se... more The feasibility of applying molecular phylogenetic methods of analysis to aligned coat-protein sequences and other molecular data derived from coat proteins or genomic sequences of members of the proposed taxonomic family of Potyviridae, is discussed. We show that comparative sequence analysis of whole coat-protein sequences may be used reliably to differentiate between sequences of closely related strains, and to show groupings of more distantly related viruses; that coat proteins of putative Potyviridae cluster according to the proposed generic divisions, and, even if some are only very distantly related, the members of the family form a cluster distinct from coat proteins of other filamentous and rod-shaped viruses. Taxonomic revisions based on perceived evolutionary relationships, and the lack of feasibility of erecting higher taxa for these viruses, are discussed.
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Papers by Ed Rybicki