Brazilian Archives of Biology and Technology, 2009
The aim of this work was to investigate the anaerobic biomass formation capable of treating vinas... more The aim of this work was to investigate the anaerobic biomass formation capable of treating vinasse from the production of sugar cane alcohol, which was evolved within an anaerobic sequencing batch biofilm reactor (ASBBR) as immobilized biomass on cubes of polyurethane foam at the temperature of 55ºC. The reactor was inoculated with mesophilic granular sludge originally treating poultry slaughterhouse wastewater. The evolution of the biofilm in the polyurethane foam matrices was assessed during seven experimental phases which were thus characterized by the changes in the organic matter concentrations as COD (1.0 to 20.0 g/L). Biomass characterization proceeded with the examination of sludge samples under optical and scanning electron microscopy. The reactor showed high microbial morphological diversity along the trial. The predominance of Methanosaeta-like cells was observed up to the organic load of 2.5 gCOD/L.d. On the other hand, Methanosarcinalike microorganisms were the predomi...
Mangrove forests are coastal intertidal ecosystems, characterized by mangrove trees growing in sl... more Mangrove forests are coastal intertidal ecosystems, characterized by mangrove trees growing in slow moving saline waters, that constitute a large portion of the coastline in the tropical and subtropical regions. The dynamic water regime created by the tides results in different micro-habitats in which microbial communities play an essential role in the functioning and maintenance of the mangrove ecosystem. However, little is known about the diversity of taxa within these micro-habitats and their functional roles, as only a small fraction of these organisms can be cultured in the laboratory. In this study, we characterized the microbial community present in three distinct regions of mangrove sediments from the Serinhaem estuary, part of the Atlantic Forest biome within the Environmental Protection Area of Pratigi. We sampled sediments from regions below the tidal waterline (submerged), intertidal regions (intertidal), and regions above the tidal waterline (seco). More than 85% of all...
Journal of Photochemistry and Photobiology B: Biology
Light biotechnology is a promising tool for enhancing recalcitrant compounds biodegradation. Xeno... more Light biotechnology is a promising tool for enhancing recalcitrant compounds biodegradation. Xenobiotics can cause a significant impact on the quality of the results achieved by sewage treatment systems due to their recalcitrance and toxicity. The optimization of bioremediation and industrial processes, aiming to increase efficiency and income is of great value. The aim of this study was to accelerate and optimize the hydrolysis of Remazol Brilliant Blue R by photo stimulating a thermophilic bacterial consortium. Three experimental groups were studied: control group; LED Group and Laser Group. The control group was exposed to the same conditions as the irradiated groups, except exposure to light. The samples were irradiated in Petri dishes with either a Laser device (λ660 nm, CW, θ = 0.04 cm2, 40 mW, 325 s, 13 J/cm2) or by a LED prototype (λ632 ± 2 nm, CW, θ = 0.5 cm2, 145 mW, 44 s, 13 J/cm2). We found that, within 48-h, statistically significant differences were observed between the irradiated and the control groups in the production of RNA, proteins, as well as in the degradation of the RBBR. It is concluded that, both Laser and LED light irradiation caused increased cellular proliferation, protein production and metabolic activity, anticipating and increasing the catabolism of the RBBR. Being the economic viability a predominant aspect for industrial propose our results indicates that photo stimulation is a low-cost booster of bioprocesses.
Journal of photochemistry and photobiology. B, Biology, 2018
Cellulose has a highly diversified architecture and its enzymatic complexes are studied for achie... more Cellulose has a highly diversified architecture and its enzymatic complexes are studied for achieving an efficient conversion and a high level of efficiency in the deconstruction of cellulolytic biomass into sugars. The aim of this investigation was to evaluate the effect of Laser or LED light in the cellulolytic activity (CMCase) and on the proliferation of the thermophilic microbial consortium used on the degradation process of a lignocellulosic biomass of green coconut shell. The irradiation protocol consisted of six Laser irradiations (λ660 ηm, 40 mW, 270 s, 13 J/cm) or LED (λ632 ± 2 ηm, 145 mW, 44 s, 13 J/cm) with 12- h time intervals in nutrient deprivation conditions. After irradiation, the consortium was inoculated into a lignocellulosic biomass (coconut fibers). Non- irradiated consortium was also inoculated and acted as control. Cell proliferation and endoglucanase activity were quantified during the experimental time. Experiments were carried out in triplicate. The result...
Surfactants and co-surfactans play an important role in enhanced oil recovery (EOR) for they impr... more Surfactants and co-surfactans play an important role in enhanced oil recovery (EOR) for they improve petroleum solubility and reduce interfacial tensions between oil, water and the rock formation. Ethanol is receiving renewed attention as potential co-surfactant because of the negative results obtained with the use of salts and alkaline substances in specific rock formations. On the other hand, sulphate-reducing bacteria (SRB) can use surfactant and co-surfactants as carbon sources and, consequently, this can increase the biological accumulation of sulphide (souring). The aim of this research is to correlate SRB activity with different concentrations of co-surfactant (ethanol) amendments as an attempt to quantifying in which concentration such compound can potentially increase or inhibit souring. The results obtained with a SRB consortium isolated from a mature oil well show that the combination of surfactant (Lauryl glucoside) and co-surfactant (ethanol) can increase SRB activity i...
Equine infectious anemia is an important infectious disease that affects equids worldwide. Contro... more Equine infectious anemia is an important infectious disease that affects equids worldwide. Control of the disease is currently based on detection of anti-p26 EIAV by Agar Gel Immunodiffusion (AGID). In this work, 62 animals were examined by AGID and nested-PCR using primers for the gag gene. Fifty-three samples (85.5%) were positive by nested-PCR, whereas only 33 samples (53%) were positive for AGID. Fifteen amplicons obtained by nested-PCR were sequenced and the aligned results subjected to phylogenetic analysis. The analysis suggests that the Brazilian EIAV form a cluster with WSU5, EIAVUK and Wyoming strains from United States.
The effect of low operating temperature and pollutant concentration on the performance of five an... more The effect of low operating temperature and pollutant concentration on the performance of five anaerobic hybrid reactors was investigated. Stable and efficient long-term (> 400 days) treatment of a cold (6-13 degrees C), volatile fatty acid (VFA)-based, wastewater was achieved at applied organic loading rates (OLRs) of 5 kg chemical oxygen demand (COD) m (-3) d (-1) with COD removal efficiencies c. 84% at 6 degrees C (sludge loading rate (SLR) 1.04-1.46 kg COD kg [VSS](-1) d (-1)). VFA-based wastewaters, containing up to 14 g ...
Brazilian Archives of Biology and Technology, 2009
The aim of this work was to investigate the anaerobic biomass formation capable of treating vinas... more The aim of this work was to investigate the anaerobic biomass formation capable of treating vinasse from the production of sugar cane alcohol, which was evolved within an anaerobic sequencing batch biofilm reactor (ASBBR) as immobilized biomass on cubes of polyurethane foam at the temperature of 55ºC. The reactor was inoculated with mesophilic granular sludge originally treating poultry slaughterhouse wastewater. The evolution of the biofilm in the polyurethane foam matrices was assessed during seven experimental phases which were thus characterized by the changes in the organic matter concentrations as COD (1.0 to 20.0 g/L). Biomass characterization proceeded with the examination of sludge samples under optical and scanning electron microscopy. The reactor showed high microbial morphological diversity along the trial. The predominance of Methanosaeta-like cells was observed up to the organic load of 2.5 gCOD/L.d. On the other hand, Methanosarcinalike microorganisms were the predomi...
Mangrove forests are coastal intertidal ecosystems, characterized by mangrove trees growing in sl... more Mangrove forests are coastal intertidal ecosystems, characterized by mangrove trees growing in slow moving saline waters, that constitute a large portion of the coastline in the tropical and subtropical regions. The dynamic water regime created by the tides results in different micro-habitats in which microbial communities play an essential role in the functioning and maintenance of the mangrove ecosystem. However, little is known about the diversity of taxa within these micro-habitats and their functional roles, as only a small fraction of these organisms can be cultured in the laboratory. In this study, we characterized the microbial community present in three distinct regions of mangrove sediments from the Serinhaem estuary, part of the Atlantic Forest biome within the Environmental Protection Area of Pratigi. We sampled sediments from regions below the tidal waterline (submerged), intertidal regions (intertidal), and regions above the tidal waterline (seco). More than 85% of all...
Journal of Photochemistry and Photobiology B: Biology
Light biotechnology is a promising tool for enhancing recalcitrant compounds biodegradation. Xeno... more Light biotechnology is a promising tool for enhancing recalcitrant compounds biodegradation. Xenobiotics can cause a significant impact on the quality of the results achieved by sewage treatment systems due to their recalcitrance and toxicity. The optimization of bioremediation and industrial processes, aiming to increase efficiency and income is of great value. The aim of this study was to accelerate and optimize the hydrolysis of Remazol Brilliant Blue R by photo stimulating a thermophilic bacterial consortium. Three experimental groups were studied: control group; LED Group and Laser Group. The control group was exposed to the same conditions as the irradiated groups, except exposure to light. The samples were irradiated in Petri dishes with either a Laser device (λ660 nm, CW, θ = 0.04 cm2, 40 mW, 325 s, 13 J/cm2) or by a LED prototype (λ632 ± 2 nm, CW, θ = 0.5 cm2, 145 mW, 44 s, 13 J/cm2). We found that, within 48-h, statistically significant differences were observed between the irradiated and the control groups in the production of RNA, proteins, as well as in the degradation of the RBBR. It is concluded that, both Laser and LED light irradiation caused increased cellular proliferation, protein production and metabolic activity, anticipating and increasing the catabolism of the RBBR. Being the economic viability a predominant aspect for industrial propose our results indicates that photo stimulation is a low-cost booster of bioprocesses.
Journal of photochemistry and photobiology. B, Biology, 2018
Cellulose has a highly diversified architecture and its enzymatic complexes are studied for achie... more Cellulose has a highly diversified architecture and its enzymatic complexes are studied for achieving an efficient conversion and a high level of efficiency in the deconstruction of cellulolytic biomass into sugars. The aim of this investigation was to evaluate the effect of Laser or LED light in the cellulolytic activity (CMCase) and on the proliferation of the thermophilic microbial consortium used on the degradation process of a lignocellulosic biomass of green coconut shell. The irradiation protocol consisted of six Laser irradiations (λ660 ηm, 40 mW, 270 s, 13 J/cm) or LED (λ632 ± 2 ηm, 145 mW, 44 s, 13 J/cm) with 12- h time intervals in nutrient deprivation conditions. After irradiation, the consortium was inoculated into a lignocellulosic biomass (coconut fibers). Non- irradiated consortium was also inoculated and acted as control. Cell proliferation and endoglucanase activity were quantified during the experimental time. Experiments were carried out in triplicate. The result...
Surfactants and co-surfactans play an important role in enhanced oil recovery (EOR) for they impr... more Surfactants and co-surfactans play an important role in enhanced oil recovery (EOR) for they improve petroleum solubility and reduce interfacial tensions between oil, water and the rock formation. Ethanol is receiving renewed attention as potential co-surfactant because of the negative results obtained with the use of salts and alkaline substances in specific rock formations. On the other hand, sulphate-reducing bacteria (SRB) can use surfactant and co-surfactants as carbon sources and, consequently, this can increase the biological accumulation of sulphide (souring). The aim of this research is to correlate SRB activity with different concentrations of co-surfactant (ethanol) amendments as an attempt to quantifying in which concentration such compound can potentially increase or inhibit souring. The results obtained with a SRB consortium isolated from a mature oil well show that the combination of surfactant (Lauryl glucoside) and co-surfactant (ethanol) can increase SRB activity i...
Equine infectious anemia is an important infectious disease that affects equids worldwide. Contro... more Equine infectious anemia is an important infectious disease that affects equids worldwide. Control of the disease is currently based on detection of anti-p26 EIAV by Agar Gel Immunodiffusion (AGID). In this work, 62 animals were examined by AGID and nested-PCR using primers for the gag gene. Fifty-three samples (85.5%) were positive by nested-PCR, whereas only 33 samples (53%) were positive for AGID. Fifteen amplicons obtained by nested-PCR were sequenced and the aligned results subjected to phylogenetic analysis. The analysis suggests that the Brazilian EIAV form a cluster with WSU5, EIAVUK and Wyoming strains from United States.
The effect of low operating temperature and pollutant concentration on the performance of five an... more The effect of low operating temperature and pollutant concentration on the performance of five anaerobic hybrid reactors was investigated. Stable and efficient long-term (> 400 days) treatment of a cold (6-13 degrees C), volatile fatty acid (VFA)-based, wastewater was achieved at applied organic loading rates (OLRs) of 5 kg chemical oxygen demand (COD) m (-3) d (-1) with COD removal efficiencies c. 84% at 6 degrees C (sludge loading rate (SLR) 1.04-1.46 kg COD kg [VSS](-1) d (-1)). VFA-based wastewaters, containing up to 14 g ...
Land-crops versus microalgae as feedstock for biodiesel: fuel quality and production potential in... more Land-crops versus microalgae as feedstock for biodiesel: fuel quality and production potential in support to industrial planning
Iracema A. Nascimento, Sheyla S. I. Marques, Iago Teles D. Cabanelas, Solange A. Pereira, Gilson C. de Carvalho, Fabio Alexandre Chinalia
1Institute of Biology, Federal University of Bahia, Campus Ondina. CEP 40.170-290. Salvador-Bahia, Brazil. 2Health Sciences Institute, Federal University of Bahia. Campus Canela, Salvador-Bahia, Brazil. Corresponding author: toiracema@gmail.com
1-Introdução-Despite claims of microalgae advantages over land-crops as feedstock, the algae-biodiesel industry is still in an emerging state. Sound knowledge of algal species for biodiesel production is limited to a few species. The diversity of climate, growth systems and exaggerated yields estimations make difficult a comparative analysis between land-crops and microalgae as feedstock. This work aims to compare both feedstock by crosschecking the oil average yields data reported [1] for commercial open-ponds and photobioreactors (PBR) in relation to the most conservative oil-yields, which were obtained by this research at laboratory standard conditions, for ten Chlorophyta species. 2-Methods-The comparison between those results and oils-yields reported in literature for ten land-crops feedstock was carried out by an integrative analysis involving (i) yields, (ii) fuel quality and (iii) compliance to biodiesel standards, based on FAME-profiling. Fuel-quality parameters were estimated by using correlative models applied to the molecular structures of FAME, and tested for compliance to internationally known biodiesel standards [2]. . 3-Results and Conclusions -Commercial algae-oil yields may achieve production ranging from 3.5 to 23.0 ton/ha/year and from 6.4 to 44.4 ton/ha/year respectively for open-ponds and photobioreactors. The variation is dependent on the producing strains, which may yield oil from 7.4 to 50.8 ton/ha/year at the most conservative laboratorial estimations. The final comparison between land-crops and microalgae, carried out through integrative statistical analyses, showed the following: (i) the laboratorial data on microalgae oil yields have been superior, while not statistically significant, in relation to the ones reported for the pilot-scale PBR system [2]. This reflects the highest control at laboratory conditions, besides the good performance of the pilot-scale growth-system taken as reference; (ii) in all the scenarios, eight among ten of the studied microalgae showed superior oil-yields than palm-tree, which is the most productive among the land-crops feedstock; (iii) in general, microalgae have surpassed land-crops in oil-yields by at least one order of magnitude; (iv) based on a cutting-off threshold equivalent to oil-yields of 40 mgl-1d-1, seven of the ten studied microalgae were identified as adequate for sustaining commercial production systems. 4-References: [1] Quinn, J.C et al.. Bioenerg. Res. 5, (2012) p 49 [2] Nascimento, IA et al. Bioenerg. Res. 6, (2013) p 1 [3] Chisti, Y. Biotechnol. Advan., 25, (2007), p 294
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Papers by Fabio Chinalia
Iracema A. Nascimento, Sheyla S. I. Marques, Iago Teles D. Cabanelas, Solange A. Pereira, Gilson C. de Carvalho, Fabio Alexandre Chinalia
1Institute of Biology, Federal University of Bahia, Campus Ondina. CEP 40.170-290. Salvador-Bahia, Brazil. 2Health Sciences Institute, Federal University of Bahia. Campus Canela, Salvador-Bahia, Brazil.
Corresponding author: toiracema@gmail.com
1-Introdução-Despite claims of microalgae advantages over land-crops as feedstock, the algae-biodiesel industry is still in an emerging state. Sound knowledge of algal species for biodiesel production is limited to a few species. The diversity of climate, growth systems and exaggerated yields estimations make difficult a comparative analysis between land-crops and microalgae as feedstock. This work aims to compare both feedstock by crosschecking the oil average yields data reported [1] for commercial open-ponds and photobioreactors (PBR) in relation to the most conservative oil-yields, which were obtained by this research at laboratory standard conditions, for ten Chlorophyta species.
2-Methods-The comparison between those results and oils-yields reported in literature for ten land-crops feedstock was carried out by an integrative analysis involving (i) yields, (ii) fuel quality and (iii) compliance to biodiesel standards, based on FAME-profiling. Fuel-quality parameters were estimated by using correlative models applied to the molecular structures of FAME, and tested for compliance to internationally known biodiesel standards [2]. .
3-Results and Conclusions -Commercial algae-oil yields may achieve production ranging from 3.5 to 23.0 ton/ha/year and from 6.4 to 44.4 ton/ha/year respectively for open-ponds and photobioreactors. The variation is dependent on the producing strains, which may yield oil from 7.4 to 50.8 ton/ha/year at the most conservative laboratorial estimations. The final comparison between land-crops and microalgae, carried out through integrative statistical analyses, showed the following: (i) the laboratorial data on microalgae oil yields have been superior, while not statistically significant, in relation to the ones reported for the pilot-scale PBR system [2]. This reflects the highest control at laboratory conditions, besides the good performance of the pilot-scale growth-system taken as reference; (ii) in all the scenarios, eight among ten of the studied microalgae showed superior oil-yields than palm-tree, which is the most productive among the land-crops feedstock; (iii) in general, microalgae have surpassed land-crops in oil-yields by at least one order of magnitude; (iv) based on a cutting-off threshold equivalent to oil-yields of 40 mgl-1d-1, seven of the ten studied microalgae were identified as adequate for sustaining commercial production systems.
4-References:
[1] Quinn, J.C et al.. Bioenerg. Res. 5, (2012) p 49
[2] Nascimento, IA et al. Bioenerg. Res. 6, (2013) p 1
[3] Chisti, Y. Biotechnol. Advan., 25, (2007), p 294