Page 1. J Bras Pneumol. 2007;33(3):323-334 323 Artigo de Revisão Diagnóstico imunológico da tuber... more Page 1. J Bras Pneumol. 2007;33(3):323-334 323 Artigo de Revisão Diagnóstico imunológico da tuberculose: problemas e estratégias para o sucesso* Immunological diagnosis of tuberculosis: problems and strategies for success ...
1Abteilung Immunologie, Universitat UJm, Albert Einstein Allee 11, 89070 Ulm, Germany 2Departamen... more 1Abteilung Immunologie, Universitat UJm, Albert Einstein Allee 11, 89070 Ulm, Germany 2Departamento de Imunologia, Universidade de Sao Paulo, Avenida Lineu Prestes 2415, 05508 Sao Paulo, Brazil ... 3Present address: Departamento de Parasitologia, Microbiologia e Imunologia, ...
Frequencies of IFNγ- and IL-4-producing spleen cells in response to Mycobacterium bovis BCG infec... more Frequencies of IFNγ- and IL-4-producing spleen cells in response to Mycobacterium bovis BCG infection were determined in C57BL6 and BALBc mice. Both mouse strains express equal innate susceptibility to M. bovis BCG (Bcgs), but differ in their NK1.1 and T-cell activities. M. bovis BCG infection induced higher frequencies (f ≈ 1500) of antigen-induced IFNγ-secreting spleen cells in C57BL6 mice as compared to BALBc mice (f ≈ 18000). Concanavalin A stimulated almost equal numbers of IFNγ-secreting cells in both mouse strains (f ≈ 150). Treatment with anti-NK1.1 mAb of M. bovis BCG-infected C57BL6 mice did not alter frequencies of IFNγ-secreting cells. Equally low numbers of antigen-induced IL-4-producing cells (f ≈ 13000) were determined in both C57BL6 and BALBc mice during M. bovis BCG infection and treatment of C57BL6 mice with anti-NK1.1 mAb had no measurable effect on IL-4 producers. Finally, frequencies of IFNγ-producing cells were markedly reduced (10-fold) in M. bovis BCG-infected TCR-β−− gene deletion mutants as compared to their heterozygous controls. Our findings verify that M. bovis BCG infection primarily induces IFNγ-secreting αβ T cells of TH1 type and show that the frequencies of these IFNγ producers differ in the two Bcgs mouse strains C57BL6 and BALBc.
Experimental autoimmune encephalomyelitis (EAE) is a murine autoimmune disease used to study mult... more Experimental autoimmune encephalomyelitis (EAE) is a murine autoimmune disease used to study multiple sclerosis (MS), a human inflammatory demyelinating disease of the central nervous system. Genistein, an isoflavonoid phytoestrogenic compound found in soy, is known to reverse clinical signs of EAE. Although genistein has some potential in clinical application, it has some disadvantages related to its chemical structure, such as rapid in vivo metabolism and a fast decline in serum after oral administration. The present work investigates the treatment of EAE by using 7-O-tetradecanoyl-genistein (TDG), a more lipophilic analog of genistein obtained by esterification. The clinical course of EAE was investigated in C57Bl/6 mice immunized with myelin oligodendrocyte glycoprotein peptide (MOG)(35-55) in complete Freund's adjuvant supplemented with Mycobacterium tuberculosis H37RA. After 14 days of MOG immunization, mice were treated with TDG for seven days. Numbers of IL-17-producing...
Page 1. J Bras Pneumol. 2007;33(3):323-334 323 Artigo de Revisão Diagnóstico imunológico da tuber... more Page 1. J Bras Pneumol. 2007;33(3):323-334 323 Artigo de Revisão Diagnóstico imunológico da tuberculose: problemas e estratégias para o sucesso* Immunological diagnosis of tuberculosis: problems and strategies for success ...
1Abteilung Immunologie, Universitat UJm, Albert Einstein Allee 11, 89070 Ulm, Germany 2Departamen... more 1Abteilung Immunologie, Universitat UJm, Albert Einstein Allee 11, 89070 Ulm, Germany 2Departamento de Imunologia, Universidade de Sao Paulo, Avenida Lineu Prestes 2415, 05508 Sao Paulo, Brazil ... 3Present address: Departamento de Parasitologia, Microbiologia e Imunologia, ...
Frequencies of IFNγ- and IL-4-producing spleen cells in response to Mycobacterium bovis BCG infec... more Frequencies of IFNγ- and IL-4-producing spleen cells in response to Mycobacterium bovis BCG infection were determined in C57BL6 and BALBc mice. Both mouse strains express equal innate susceptibility to M. bovis BCG (Bcgs), but differ in their NK1.1 and T-cell activities. M. bovis BCG infection induced higher frequencies (f ≈ 1500) of antigen-induced IFNγ-secreting spleen cells in C57BL6 mice as compared to BALBc mice (f ≈ 18000). Concanavalin A stimulated almost equal numbers of IFNγ-secreting cells in both mouse strains (f ≈ 150). Treatment with anti-NK1.1 mAb of M. bovis BCG-infected C57BL6 mice did not alter frequencies of IFNγ-secreting cells. Equally low numbers of antigen-induced IL-4-producing cells (f ≈ 13000) were determined in both C57BL6 and BALBc mice during M. bovis BCG infection and treatment of C57BL6 mice with anti-NK1.1 mAb had no measurable effect on IL-4 producers. Finally, frequencies of IFNγ-producing cells were markedly reduced (10-fold) in M. bovis BCG-infected TCR-β−− gene deletion mutants as compared to their heterozygous controls. Our findings verify that M. bovis BCG infection primarily induces IFNγ-secreting αβ T cells of TH1 type and show that the frequencies of these IFNγ producers differ in the two Bcgs mouse strains C57BL6 and BALBc.
Experimental autoimmune encephalomyelitis (EAE) is a murine autoimmune disease used to study mult... more Experimental autoimmune encephalomyelitis (EAE) is a murine autoimmune disease used to study multiple sclerosis (MS), a human inflammatory demyelinating disease of the central nervous system. Genistein, an isoflavonoid phytoestrogenic compound found in soy, is known to reverse clinical signs of EAE. Although genistein has some potential in clinical application, it has some disadvantages related to its chemical structure, such as rapid in vivo metabolism and a fast decline in serum after oral administration. The present work investigates the treatment of EAE by using 7-O-tetradecanoyl-genistein (TDG), a more lipophilic analog of genistein obtained by esterification. The clinical course of EAE was investigated in C57Bl/6 mice immunized with myelin oligodendrocyte glycoprotein peptide (MOG)(35-55) in complete Freund's adjuvant supplemented with Mycobacterium tuberculosis H37RA. After 14 days of MOG immunization, mice were treated with TDG for seven days. Numbers of IL-17-producing...
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