Papers by Danillo M Porfírio
Brazilian Journal of Health Review, 2020
A Leptospirose e uma doenca febril aguda causada pela espiroqueta Leptospira interrogans. Sua tra... more A Leptospirose e uma doenca febril aguda causada pela espiroqueta Leptospira interrogans. Sua transmissao ocorre, principalmente, atraves do contato com a agua ou lama de enchentes contaminadas com urina de animais portadores, sobretudo os ratos. Nessa otica, foi realizado um estudo descritivo qualitativo com analise de dados secundarios disponiveis no Departamento de Informatica do SUS (DATASUS), sobre o perfil clinico-epidemiologico leptospirose no estado do Para entre os anos de 2012 a 2017. Os dados pesquisados foram analisados de acordo com as variaveis: faixa etaria, sexo, raca e evolucao clinica. Apos a analise dos dados, foram evidenciados um total de 782 notificacoes de casos da doenca, no periodo analisado. O grupo etario mais afetado foi o de 20 a 39 anos de idade. Em relacao ao sexo, observou-se predominio nos homens. Quanto a variavel cor, os pardos tiveram 73,9% indice de contagio. Do total de pessoas diagnosticadas com a doenca, 11,5% evoluiram para obito. Observou-se...
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Frontiers in Pharmacology, 2020
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Prevalência de Doença de Chagas em Idosos no Estado do Pará: Uma Análise Retrospectiva, 2020
Chagas disease is an anthropozoonosis caused by the protozoan Tripanosoma cruzi, having as a vect... more Chagas disease is an anthropozoonosis caused by the protozoan Tripanosoma cruzi, having as a vector the triatomine hematophagous insect, commonly called "barber bug". The disease progresses with an acute phase that can persist if there is no timely diagnosis and treatment, characterizing a chronic phase. As chagas disease is the second most common cause of death in elderly Brazilians among infectious and parasitic diseases, the association of the disease with the aging process deserves emphasis. Thus, the objective of this study was to verify the prevalence of Chagas disease in elderly people in the state of Pará and its contribution to medical training. This was an analytical, observational, cross-sectional and retrospective study, whose data were obtained by consulting the SINAN database (Notifiable Disease Information System) made available by the Department of Informatics of the Unified Health System (DATASUS). The study population consisted of all cases of Chagas disease in the age group from 60 to 80 years old in the state of Pará in the period from 2010 to 2017. The variables analyzed were: disease, notification status, mode of transmission, gender and age group. In this period, 173 acute cases of Chagas diseases in the State of Pará were reported in the corresponding age group, with predominance in the cities of Ananindeua (23.12%), Belém (20.23%) and Abaetetuba (14.45%). The disease had higher rates in 2012 and 2017. The age group most affected was 70 - 79 years old (34.68%), in which there was a predominance of females (51.66%). However, under a general analysis of the reported cases, the male gender predominated with 90 cases (52.02%). As for race, most of those affected by the disease declared themselves to be brown (75.72%). The main probable mode of transmission of the disease was oral (75.14%), with home as the main probable site of infection. Regarding the confirmatory criterion, there was a predominance of the laboratory confirmation (97.68%). Regarding the evolution of the disease, 148 confirmed cases evolved to “alive” (85.54%) and only 9 to death. Thus, the occurrence of Chagas disease in the elderly is a serious public health problem, being caused mainly through the oral transmission of the pathogen. In addition, about sex, there was a balance in the number of acute cases with slight male prevalence. Thus, the factors associated with the disease presented can be manipulated by the public authorities and must be considered when planning effective measures for their control.
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Frontiers in Pharmacology, 2020
Fish use spatial cognition based on allocentric cues to navigate, but little is known about how e... more Fish use spatial cognition based on allocentric cues to navigate, but little is known about how environmental enrichment (EE) affects learning and memory in correlation with hematological changes or gene expression in the fish brain. Here we investigated these questions in Colossoma macropomum (Teleostei). Fish were housed for 192 days in either EE or in an impoverished environment (IE) aquarium. EE contained toys, natural plants, and a 12-h/day water stream for voluntary exercise, whereas IE had no toys, plants, or water stream. A third plus maze aquarium was used for spatial and object recognition tests. Compared with IE, the EE fish showed greater learning rates, body length, and body weight. After behavioral tests, whole brain tissue was taken, stored in RNA-later, and then homogenized for DNA sequencing after conversion of isolated RNA. To compare read mapping and gene expression profiles across libraries for neurotranscriptome differential expression, we mapped back RNA-seq reads to the C. macropomum de novo assembled transcriptome. The results showed significant differential behavior, cell counts and gene expression in EE and IE individuals. As compared with IE, we found a greater number of cells in the telencephalon of individuals maintained in EE but no significant difference in the tectum opticum, suggesting differential plasticity in these areas. A total of 107,669 transcripts were found that ultimately yielded 64 differentially expressed transcripts between IE and EE brains. Another group of adult fish growing in aquaculture conditions were either subjected to exercise using running water flow or maintained
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Original Research by Danillo M Porfírio
Fish use spatial cognition based on allocentric cues to navigate, but little is known about how e... more Fish use spatial cognition based on allocentric cues to navigate, but little is known about how environmental enrichment (EE) affects learning and memory in correlation with hematological changes or gene expression in the fish brain. Here we investigated these questions in Colossoma macropomum (Teleostei). Fish were housed for 192 days in either EE or in an impoverished environment (IE) aquarium. EE contained toys, natural plants, and a 12-h/day water stream for voluntary exercise, whereas IE had no toys, plants, or water stream. A third plus maze aquarium was used for spatial and object recognition tests. Compared with IE, the EE fish showed greater learning rates, body length, and body weight. After behavioral tests, whole brain tissue was taken, stored in RNA-later, and then homogenized for DNA sequencing after conversion of isolated RNA. To compare read mapping and gene expression profiles across libraries for neurotranscriptome differential expression, we mapped back RNA-seq reads to the C. macropomum de novo assembled transcriptome. The results showed significant differential behavior, cell counts and gene expression in EE and IE individuals. As compared with IE, we found a greater number of cells in the telencephalon of individuals maintained in EE but no significant difference in the tectum opticum, suggesting differential plasticity in these areas. A total of 107,669 transcripts were found that ultimately yielded 64 differentially expressed transcripts between IE and EE brains. Another group of adult fish growing in aquaculture conditions were either subjected to exercise using running water flow or maintained sedentary. Flow cytometry analysis of peripheral blood showed a significantly higher density of lymphocytes, and platelets but no significant differences in erythrocytes and granulocytes. Thus, under the influence of contrasting environments, our findings showed differential changes at the behavioral, cellular, and molecular levels. We propose that the differential expression of selected transcripts, number of telencephalic cell counts, learning and memory performance, and selective hematological cell changes may be part of Teleostei adaptive physiological responses triggered by EE visuospatial and somatomotor stimulation. Our findings suggest abundant differential gene expression changes depending on environment and provide a basis for exploring gene regulation mechanisms under EE in C. macropomum.
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Conference Presentations by Danillo M Porfírio
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2017
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2017
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Papers by Danillo M Porfírio
Original Research by Danillo M Porfírio
Conference Presentations by Danillo M Porfírio
Drafts by Danillo M Porfírio