Electropolymerized nanoporous polymeric SPME coatings: preparation and characterization by small ... more Electropolymerized nanoporous polymeric SPME coatings: preparation and characterization by small angle X-ray scattering and scanning electron microscopy
1 Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernic... more 1 Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin Str., 87100 Torun, Poland 2 Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, 85 Umultowska Str., 61614 Poznan, Poland 3 Department of Crystallography, Faculty of Chemistry, Maria Curie-Sklodowska University, 3 Maria Curie-Sklodowska Str., 20031 Lublin, Poland
HIV-1 infection of macrophages results in cell survival and viral persistence. Although it has be... more HIV-1 infection of macrophages results in cell survival and viral persistence. Although it has been shown that infecting virus deregulates macrophage function, the mechanism for cell survival remains unknown. Moreover, HIV infection is often complicated by concurrent use of illicit drugs and may potentiate already adverse effect of viral infection. Taken together, it is very difficult to manage these two devastating factors. Therefore, understanding molecular mechanisms is of the utmost importance at this stage of our fight against HIV.
A new approach to the rapid determination of amoxicillin (AMO) in human plasma followed by solid ... more A new approach to the rapid determination of amoxicillin (AMO) in human plasma followed by solid phase microextraction (SPME) fiber coatings based on conducting polymers (polypyrrole and polythiophene) and high performance liquid chromatography (HPLC) has been described. The porous structures of the electrochemically deposited polymer coatings have been characterized by scanning electron microscopy (SEM). The experimental parameters relating to the extraction efficiency of the SPME fibers such as pH, extraction time and desorption conditions (solvents, time) were studied and selected. The SPME/HPLC-UV method was linear over a working range of 1-50 μg ml(-1). The inter-day accuracy (expressed as coefficients of variations, CVs) was less than 15% and precision (expressed as the relative standard deviations, RSDs) with percentage values was less than 5.9%. Amoxicillin was found to be stable in the human plasma at room temperature (20 °C) within 8 hours. The developed method was successfully applied to the analysis of real human plasma samples. The limit of detection and limit of quantification for amoxicillin in plasma were 1.21 μg ml(-1) and 3.48 μg ml(-1), respectively.
Electropolymerized nanoporous polymeric SPME coatings: preparation and characterization by small ... more Electropolymerized nanoporous polymeric SPME coatings: preparation and characterization by small angle X-ray scattering and scanning electron microscopy
1 Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernic... more 1 Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 7 Gagarin Str., 87100 Torun, Poland 2 Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, 85 Umultowska Str., 61614 Poznan, Poland 3 Department of Crystallography, Faculty of Chemistry, Maria Curie-Sklodowska University, 3 Maria Curie-Sklodowska Str., 20031 Lublin, Poland
HIV-1 infection of macrophages results in cell survival and viral persistence. Although it has be... more HIV-1 infection of macrophages results in cell survival and viral persistence. Although it has been shown that infecting virus deregulates macrophage function, the mechanism for cell survival remains unknown. Moreover, HIV infection is often complicated by concurrent use of illicit drugs and may potentiate already adverse effect of viral infection. Taken together, it is very difficult to manage these two devastating factors. Therefore, understanding molecular mechanisms is of the utmost importance at this stage of our fight against HIV.
A new approach to the rapid determination of amoxicillin (AMO) in human plasma followed by solid ... more A new approach to the rapid determination of amoxicillin (AMO) in human plasma followed by solid phase microextraction (SPME) fiber coatings based on conducting polymers (polypyrrole and polythiophene) and high performance liquid chromatography (HPLC) has been described. The porous structures of the electrochemically deposited polymer coatings have been characterized by scanning electron microscopy (SEM). The experimental parameters relating to the extraction efficiency of the SPME fibers such as pH, extraction time and desorption conditions (solvents, time) were studied and selected. The SPME/HPLC-UV method was linear over a working range of 1-50 μg ml(-1). The inter-day accuracy (expressed as coefficients of variations, CVs) was less than 15% and precision (expressed as the relative standard deviations, RSDs) with percentage values was less than 5.9%. Amoxicillin was found to be stable in the human plasma at room temperature (20 °C) within 8 hours. The developed method was successfully applied to the analysis of real human plasma samples. The limit of detection and limit of quantification for amoxicillin in plasma were 1.21 μg ml(-1) and 3.48 μg ml(-1), respectively.
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