Eric Yirenkyi Danquah is a Professor of Plant Genetics at the Department of Crop Science of the College of Basic and Applied Sciences, University of Ghana. He is a recipient of the University of Ghana Distinguished Award for Meritorious Service, 2013 and a member of the Academic Board of Planet Earth Institute, UK. He holds a BSc. degree in Agriculture (Crop Science) from the University of Ghana and an MPhil degree in Plant Breeding and a PhD in Genetics from the University of Cambridge, UK. He is a former Dean of International Programmes of the University of Ghana. Currently, he serves as the Director of the West Africa Centre for Crop Improvement, a flagship PhD programme established in the University in 2007 as a consequence of his shared vision and leadership to train a new generation of plant breeders to develop improved varieties of the staple crops of West and Central Africa. He also serves as the Director of the Biotechnology Centre, University of Ghana.
ABSTRACT A range of three generations from each of three populations of Cambridge Composite Cross... more ABSTRACT A range of three generations from each of three populations of Cambridge Composite Cross Five of barley spanning a period of 18 years of cultivation in Cambridge was evaluated for grain yield over 2 years in the field in 1991 and 1992. The design of the experiment was a randomized complete block with two replications. In 1991, the yield of the best composite cross generation was 78% that of Atem, a commercial variety bred for N.W. European conditions. In the drought-affected year, 1992, three of the composite cross generations performed better than Atem. It is suggested that composite cross populations may be useful in deriving lines for low-input agriculture.
418. hygromycin, kanamycin, cefotaxime and carbenicillin were tested for their suitability for in... more 418. hygromycin, kanamycin, cefotaxime and carbenicillin were tested for their suitability for in vitro culture of S. gila. Concentrations used \'Vcre2.5, 5.0, 10.0,20.0 and 30.0 mg/I for G4 I8 and hygromycin: and 100, ISO, 200, 250 and 300 mg/I for kanamycin. Cefotaxime and carbenicillin concentrations were 300, 500 arid 700 mg/I with or without zeatin in the medium. Hypocotyl explants tolerated up to 20 mg/I G418 while regeneration of cotyledon and leaf explants was inhibited at 2.5 mg/1.Callus development was high on medium containing 10mg/I hygromycin but declined at 20 mg/1.Callus production in hypocotyl explants was normal on ISO mg/I kanamycin while shoot prodliction declined with increasing concentrations. No regeneration occurred in cotyledon explants cultured on 100mg/l kanamycin. Without zeatin, increasing concentrations of carbenicillin did not affect regeneration but zeatin supplementation further enhance it. Cefotazime improved cotyledon explant enlongation but adv...
Single Nucleotide Polymorphism (SNP) markers were used in characterization of 113 cowpea accessio... more Single Nucleotide Polymorphism (SNP) markers were used in characterization of 113 cowpea accessions comprising of 108 from Ghana and 5 from abroad. Leaf tissues from plants cultivated at the University of Ghana were genotyped at KBioscience in the United Kingdom. Data was generated for 477 SNPs, out of which 458 revealed polymorphism. The results were used to analyze genetic dissimilarity among the accessions using Darwin 5 software. The markers discriminated among all of the cowpea accessions and the dissimilarity values which ranged from 0.006 to 0.63 were used for factorial plot. Unexpected high levels of heterozygosity were observed on some of the accessions. Accessions known to be closely related clustered together in a dendrogram drawn with WPGMA method. A maximum length sub-tree which comprised of 48 core accessions was constructed. The software package structure was used to separate accessions into three groups, and the programme correctly identified varieties that were known hybrids. The hybrids were those accessions with numerous heterozygous loci. The structure plot showed closely related accessions with similar genome patterns. The SNP markers were more efficient in discriminating among the cowpea germplasm than morphological, seed protein polymorphism and simple sequence repeat studies reported earlier on the same collection.
ABSTRACT Summary Diversity studies of Echinochloa spp. are complicated by problems in taxonomy an... more ABSTRACT Summary Diversity studies of Echinochloa spp. are complicated by problems in taxonomy and species identification, caused by the existence of morphologically intergrading types. Six amplified fragment length polymorphism (AFLP) primer combinations and five microsatellites were used to assess variation in 24 samples morphologically identified as E. crus-galli, E. colona and E. crus-pavonis, from Bangladesh, India, Colombia, Costa Rica, Côte d'Ivoire and Philippines. Out of 909 AFLP bands generated, 775 were polymorphic. Genotype diversity for the microsatellites ranged from 0.28 to 0.72. Similarity matrices were calculated using Jaccard coefficient, and input into cluster and principal coordinates analyses. AFLP and microsatellite results were highly correlated. Echinochloa crus-pavonis and E. crus-galli were intermixed, consistent with the view that E. crus-galli occurs as numerous intergrading races in the four countries (Bangladesh, India, Côte d'Ivoire and Philippines). The E. colona samples clustered as a distinct group. In 15 samples of E. crus-pavonis collected from rice fields in a valley in Côte d'Ivoire (over a 2-km distance), four different genotypes were found in a 4 m × 4 m area. These results suggest that AFLPs and SSRs may be useful not only for discriminating genotypes and studying population structure but also for helping to resolve taxonomic relationships in Echinochloa spp.
... Devi-ation from Hardy–Weinberg equilibrium was calculated using GENEPOP v4.0.10 (Raymond and ... more ... Devi-ation from Hardy–Weinberg equilibrium was calculated using GENEPOP v4.0.10 (Raymond and Rousset 1995; Rousset 2008). Data were checked for evidence of null alleles and scoring errors with MICRO-CHECKER v2.2.3 (van Oosterhout et al. ...
ABSTRACT A range of three generations from each of three populations of Cambridge Composite Cross... more ABSTRACT A range of three generations from each of three populations of Cambridge Composite Cross Five of barley spanning a period of 18 years of cultivation in Cambridge was evaluated for grain yield over 2 years in the field in 1991 and 1992. The design of the experiment was a randomized complete block with two replications. In 1991, the yield of the best composite cross generation was 78% that of Atem, a commercial variety bred for N.W. European conditions. In the drought-affected year, 1992, three of the composite cross generations performed better than Atem. It is suggested that composite cross populations may be useful in deriving lines for low-input agriculture.
418. hygromycin, kanamycin, cefotaxime and carbenicillin were tested for their suitability for in... more 418. hygromycin, kanamycin, cefotaxime and carbenicillin were tested for their suitability for in vitro culture of S. gila. Concentrations used \'Vcre2.5, 5.0, 10.0,20.0 and 30.0 mg/I for G4 I8 and hygromycin: and 100, ISO, 200, 250 and 300 mg/I for kanamycin. Cefotaxime and carbenicillin concentrations were 300, 500 arid 700 mg/I with or without zeatin in the medium. Hypocotyl explants tolerated up to 20 mg/I G418 while regeneration of cotyledon and leaf explants was inhibited at 2.5 mg/1.Callus development was high on medium containing 10mg/I hygromycin but declined at 20 mg/1.Callus production in hypocotyl explants was normal on ISO mg/I kanamycin while shoot prodliction declined with increasing concentrations. No regeneration occurred in cotyledon explants cultured on 100mg/l kanamycin. Without zeatin, increasing concentrations of carbenicillin did not affect regeneration but zeatin supplementation further enhance it. Cefotazime improved cotyledon explant enlongation but adv...
Single Nucleotide Polymorphism (SNP) markers were used in characterization of 113 cowpea accessio... more Single Nucleotide Polymorphism (SNP) markers were used in characterization of 113 cowpea accessions comprising of 108 from Ghana and 5 from abroad. Leaf tissues from plants cultivated at the University of Ghana were genotyped at KBioscience in the United Kingdom. Data was generated for 477 SNPs, out of which 458 revealed polymorphism. The results were used to analyze genetic dissimilarity among the accessions using Darwin 5 software. The markers discriminated among all of the cowpea accessions and the dissimilarity values which ranged from 0.006 to 0.63 were used for factorial plot. Unexpected high levels of heterozygosity were observed on some of the accessions. Accessions known to be closely related clustered together in a dendrogram drawn with WPGMA method. A maximum length sub-tree which comprised of 48 core accessions was constructed. The software package structure was used to separate accessions into three groups, and the programme correctly identified varieties that were known hybrids. The hybrids were those accessions with numerous heterozygous loci. The structure plot showed closely related accessions with similar genome patterns. The SNP markers were more efficient in discriminating among the cowpea germplasm than morphological, seed protein polymorphism and simple sequence repeat studies reported earlier on the same collection.
ABSTRACT Summary Diversity studies of Echinochloa spp. are complicated by problems in taxonomy an... more ABSTRACT Summary Diversity studies of Echinochloa spp. are complicated by problems in taxonomy and species identification, caused by the existence of morphologically intergrading types. Six amplified fragment length polymorphism (AFLP) primer combinations and five microsatellites were used to assess variation in 24 samples morphologically identified as E. crus-galli, E. colona and E. crus-pavonis, from Bangladesh, India, Colombia, Costa Rica, Côte d'Ivoire and Philippines. Out of 909 AFLP bands generated, 775 were polymorphic. Genotype diversity for the microsatellites ranged from 0.28 to 0.72. Similarity matrices were calculated using Jaccard coefficient, and input into cluster and principal coordinates analyses. AFLP and microsatellite results were highly correlated. Echinochloa crus-pavonis and E. crus-galli were intermixed, consistent with the view that E. crus-galli occurs as numerous intergrading races in the four countries (Bangladesh, India, Côte d'Ivoire and Philippines). The E. colona samples clustered as a distinct group. In 15 samples of E. crus-pavonis collected from rice fields in a valley in Côte d'Ivoire (over a 2-km distance), four different genotypes were found in a 4 m × 4 m area. These results suggest that AFLPs and SSRs may be useful not only for discriminating genotypes and studying population structure but also for helping to resolve taxonomic relationships in Echinochloa spp.
... Devi-ation from Hardy–Weinberg equilibrium was calculated using GENEPOP v4.0.10 (Raymond and ... more ... Devi-ation from Hardy–Weinberg equilibrium was calculated using GENEPOP v4.0.10 (Raymond and Rousset 1995; Rousset 2008). Data were checked for evidence of null alleles and scoring errors with MICRO-CHECKER v2.2.3 (van Oosterhout et al. ...
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