The aims of the study were to evaluate the influence of culture media on biofilm formation byC. a... more The aims of the study were to evaluate the influence of culture media on biofilm formation byC. albicans, C. glabrata, C. krusei,andC. parapsilosisand to investigate the responses of sessile cells to antifungals and reactive oxygen species (ROS) as compared to planktonic cells. For biofilm formation, theCandidaspecies were grown at different periods of time in YP or YNB media supplemented or not with 0.2 or 2% glucose. Sessile and planktonic cells were exposed to increasing concentrations of antifungals, H2O2, menadione or silver nanoparticles (AgNPs). Biofilms were observed by scanning electron microscopy (SEM) and quantified by the XTT assay.C. albicansformed biofilms preferentially in YPD containing 2% glucose (YPD/2%),C. glabratain glucose-free YNB or supplemented with 0.2% glucose (YNB/0.2%), whileC. kruseiandC. parapsilosispreferred YP, YPD/0.2%, and YPD/2%. Interestingly, onlyC. albicansproduced an exopolymeric matrix. This is the first report dealing with thein vitroeffect o...
Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology, 1997
We report the use of an extremely low frequency magnetic field (ELF-MF, 60 Hz, 10 Grms) to stimul... more We report the use of an extremely low frequency magnetic field (ELF-MF, 60 Hz, 10 Grms) to stimulate cultured chromaffin cells to determine the possible changes that may occur in their electrical properties. The results showed that ELF-MF not only facilitated neurite outgrowth, but also formation of neurite varicosity with high concentration of catecholamines. This report also shows for the first time that a greater number of cultured chromaffin cells differentiated by ELF-MF have spontaneous extracellular electrical activity and that their firing frequency is higher than that seen in non-stimulated cells.
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference, 2010
Biomedical engineering at the Universidad Nacional Autónoma de Mexico (UNAM - National Autonomous... more Biomedical engineering at the Universidad Nacional Autónoma de Mexico (UNAM - National Autonomous University of Mexico) has been developing slowly compared to other local universities. A BME-closing module was established as an elective one for the bachelors of electronic and computer engineering degrees and it has been offered recently. This approach enables the students to apply the principles of physics and engineering in a biomedical context. The popularity of the BME-closing module is growing and more students are opting for this module. This paper presents the BME activities at the UNAM and the experience of the elective module.
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference, 2011
Providing appropriate cardio-pulmonary reanimation after cardio-pulmonary arrest is paramount for... more Providing appropriate cardio-pulmonary reanimation after cardio-pulmonary arrest is paramount for survival. An effective and low-cost approach to learn and practice the cardio-pulmonary reanimation is through a computerized life-size patient simulator. The present work describes the development of a patient simulator for the Centre of Education and Certification of Medical Aptitudes (CECAM) from the UNAM's Faculty of Medicine. This patient simulator has many new and innovative features, such real-time feedback to the medical student, which improves the whole teaching/learning experience.
2005 IEEE Engineering in Medicine and Biology 27th Annual Conference, 2005
The Strathclyde Upper-Limb Activity Monitor (SULAM) was used to assess real-world upper-limb acti... more The Strathclyde Upper-Limb Activity Monitor (SULAM) was used to assess real-world upper-limb activity. The SULAM consists of an electro-hydraulic activity-sensor which measures the vertical displacement of the wrist in relation to the shoulder. The aims of this study were to obtain a profile of upper-limb activity in two different populations (able-bodied participants and stroke patients) Ten able-bodied volunteers and ten stroke patients-wore the SULAM while performing their everyday activities. The outcome measures were movement time, its distribution in five vertical ranges, bimanual and unimanual movement time. There was a difference in the use of both upper-limbs for both groups, favouring the dominant/unaffected arm. This difference was only in two of the five ranges (chest to shoulder and shoulder to head for able-bodied participants; waist to chest and chest to shoulder for stroke patients). Bimanual movement was greater than unimanual movement for able-bodied participants whereas unimanual movement was greater than bimanual movement for stroke patients.
ABSTRACT The use of the Beer?Lambert law in spectroscopy is the core of standard methods for dete... more ABSTRACT The use of the Beer?Lambert law in spectroscopy is the core of standard methods for determining a chromophore concentration in a solution. Its application requires an understanding about interaction of light with a colored solution and the use of light emission and light detection devices. We build here a simple electronic circuit formed of light-emitting diodes and light-dependent resistors that can be used for measuring chromophore concentrations during experiments focused on chemical kinetics: decolorizing of phenolphthalein in a basic solution, and reduction of methylene blue by ascorbic acid. From the chemical point of view, the goal is to determine experimentally the rate law. In addition, students understand, using inexpensive electronics, the basic design of a spectrophotometer to achieve successful measurements of absorbance.
We propose a hypothesis suggesting that the most prominent experiences occurring during wakefulne... more We propose a hypothesis suggesting that the most prominent experiences occurring during wakefulness activate specific clusters of neurons related to such experiences. These neurons could possibly then evoke the release of various types of sleep-inducing molecules, thereby causing different patterns of sleep architecture. In this study, we therefore sought to determine whether manipulations of behavior during wakefulness, such as forced wakefulness induced by gentle handling, forced wakefulness associated with a stressful condition such as immobilization, or forced wakefulness associated with excess intake of palatable food, could result in a variation of Fos immunoreactivity in selective brain structures and could also result in different sleep and EEG power density patterns. The results showed that the sleep-wake cycle of rats after all the experimental manipulations was different not only with respect to the control group but also among themselves. Additionally, power spectrum analysis showed an increase of 0.25-4.0 Hz in all experimental manipulations, whereas the 4.25-8.0 Hz increase occurred only in the situation of forced wakefulness plus stress. The Fos induction showed activation of cell clusters in cortical areas and telencephalic centers, in several hypothalamic nuclei, in monoaminergic cell groups, and in brain stem nuclei. The density of Fos-immunoreactive neurons varied in relation to the different paradigms of forced wakefulness. These results suggest that activation of cell clusters in the brain are related to the type of manipulation imposed on the rat during wakefulness and that such variation in cell activation prior to sleep may be associated with sleep architecture and EEG power.
The aims of the study were to evaluate the influence of culture media on biofilm formation byC. a... more The aims of the study were to evaluate the influence of culture media on biofilm formation byC. albicans, C. glabrata, C. krusei,andC. parapsilosisand to investigate the responses of sessile cells to antifungals and reactive oxygen species (ROS) as compared to planktonic cells. For biofilm formation, theCandidaspecies were grown at different periods of time in YP or YNB media supplemented or not with 0.2 or 2% glucose. Sessile and planktonic cells were exposed to increasing concentrations of antifungals, H2O2, menadione or silver nanoparticles (AgNPs). Biofilms were observed by scanning electron microscopy (SEM) and quantified by the XTT assay.C. albicansformed biofilms preferentially in YPD containing 2% glucose (YPD/2%),C. glabratain glucose-free YNB or supplemented with 0.2% glucose (YNB/0.2%), whileC. kruseiandC. parapsilosispreferred YP, YPD/0.2%, and YPD/2%. Interestingly, onlyC. albicansproduced an exopolymeric matrix. This is the first report dealing with thein vitroeffect o...
Comparative biochemistry and physiology. Part C, Pharmacology, toxicology & endocrinology, 1997
We report the use of an extremely low frequency magnetic field (ELF-MF, 60 Hz, 10 Grms) to stimul... more We report the use of an extremely low frequency magnetic field (ELF-MF, 60 Hz, 10 Grms) to stimulate cultured chromaffin cells to determine the possible changes that may occur in their electrical properties. The results showed that ELF-MF not only facilitated neurite outgrowth, but also formation of neurite varicosity with high concentration of catecholamines. This report also shows for the first time that a greater number of cultured chromaffin cells differentiated by ELF-MF have spontaneous extracellular electrical activity and that their firing frequency is higher than that seen in non-stimulated cells.
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference, 2010
Biomedical engineering at the Universidad Nacional Autónoma de Mexico (UNAM - National Autonomous... more Biomedical engineering at the Universidad Nacional Autónoma de Mexico (UNAM - National Autonomous University of Mexico) has been developing slowly compared to other local universities. A BME-closing module was established as an elective one for the bachelors of electronic and computer engineering degrees and it has been offered recently. This approach enables the students to apply the principles of physics and engineering in a biomedical context. The popularity of the BME-closing module is growing and more students are opting for this module. This paper presents the BME activities at the UNAM and the experience of the elective module.
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference, 2011
Providing appropriate cardio-pulmonary reanimation after cardio-pulmonary arrest is paramount for... more Providing appropriate cardio-pulmonary reanimation after cardio-pulmonary arrest is paramount for survival. An effective and low-cost approach to learn and practice the cardio-pulmonary reanimation is through a computerized life-size patient simulator. The present work describes the development of a patient simulator for the Centre of Education and Certification of Medical Aptitudes (CECAM) from the UNAM's Faculty of Medicine. This patient simulator has many new and innovative features, such real-time feedback to the medical student, which improves the whole teaching/learning experience.
2005 IEEE Engineering in Medicine and Biology 27th Annual Conference, 2005
The Strathclyde Upper-Limb Activity Monitor (SULAM) was used to assess real-world upper-limb acti... more The Strathclyde Upper-Limb Activity Monitor (SULAM) was used to assess real-world upper-limb activity. The SULAM consists of an electro-hydraulic activity-sensor which measures the vertical displacement of the wrist in relation to the shoulder. The aims of this study were to obtain a profile of upper-limb activity in two different populations (able-bodied participants and stroke patients) Ten able-bodied volunteers and ten stroke patients-wore the SULAM while performing their everyday activities. The outcome measures were movement time, its distribution in five vertical ranges, bimanual and unimanual movement time. There was a difference in the use of both upper-limbs for both groups, favouring the dominant/unaffected arm. This difference was only in two of the five ranges (chest to shoulder and shoulder to head for able-bodied participants; waist to chest and chest to shoulder for stroke patients). Bimanual movement was greater than unimanual movement for able-bodied participants whereas unimanual movement was greater than bimanual movement for stroke patients.
ABSTRACT The use of the Beer?Lambert law in spectroscopy is the core of standard methods for dete... more ABSTRACT The use of the Beer?Lambert law in spectroscopy is the core of standard methods for determining a chromophore concentration in a solution. Its application requires an understanding about interaction of light with a colored solution and the use of light emission and light detection devices. We build here a simple electronic circuit formed of light-emitting diodes and light-dependent resistors that can be used for measuring chromophore concentrations during experiments focused on chemical kinetics: decolorizing of phenolphthalein in a basic solution, and reduction of methylene blue by ascorbic acid. From the chemical point of view, the goal is to determine experimentally the rate law. In addition, students understand, using inexpensive electronics, the basic design of a spectrophotometer to achieve successful measurements of absorbance.
We propose a hypothesis suggesting that the most prominent experiences occurring during wakefulne... more We propose a hypothesis suggesting that the most prominent experiences occurring during wakefulness activate specific clusters of neurons related to such experiences. These neurons could possibly then evoke the release of various types of sleep-inducing molecules, thereby causing different patterns of sleep architecture. In this study, we therefore sought to determine whether manipulations of behavior during wakefulness, such as forced wakefulness induced by gentle handling, forced wakefulness associated with a stressful condition such as immobilization, or forced wakefulness associated with excess intake of palatable food, could result in a variation of Fos immunoreactivity in selective brain structures and could also result in different sleep and EEG power density patterns. The results showed that the sleep-wake cycle of rats after all the experimental manipulations was different not only with respect to the control group but also among themselves. Additionally, power spectrum analysis showed an increase of 0.25-4.0 Hz in all experimental manipulations, whereas the 4.25-8.0 Hz increase occurred only in the situation of forced wakefulness plus stress. The Fos induction showed activation of cell clusters in cortical areas and telencephalic centers, in several hypothalamic nuclei, in monoaminergic cell groups, and in brain stem nuclei. The density of Fos-immunoreactive neurons varied in relation to the different paradigms of forced wakefulness. These results suggest that activation of cell clusters in the brain are related to the type of manipulation imposed on the rat during wakefulness and that such variation in cell activation prior to sleep may be associated with sleep architecture and EEG power.
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Papers by Arturo Vega Gonzalez