Shafiquzzaman Siddiquee
BIOGRAPHICAL DETAILS
Assoc. Prof. Dr. Md Shafiquzzaman Siddiquee has received his MSc (Molecular Microbiology) and PhD (Bioelectrochemistry) degrees in 2008 and 2010, respectively, from University Putra Malaysia (UPM), Malaysia. Given his achievements, Dr. Siddiquee had received a Best Post Graduate Award, 2011 from UPM for being the most outstanding postgraduate student of the year. Dr. Siddiquee has started his independent academic career as a senior lecturer after that got promotion as an Associate Professor at the Biotechnology Research Institute, University Malaysia Sabah. He is a head of the Biosensor Research Group at the BRI UMS. He has involved in varying types of research areas such as chemo- or/ bio- sensors, and optical sensors based research, production of biofertilizers from oil palm fibres and rice straw, fungal biodiversity, removal of heavy metal ions from sediments and waste water. Currently he has been supervising above 12 post-graduate students (PhD and Master programs) as well as 12 undergraduates and industrial students. He has been published more than 50 papers in high impact journals, 2 books, 8 book chapters and 22 proceeding papers. His findings have yielded, so far, 4 patent, 5 invited speakers and 32 research awards, including notable award of MOHE entrepreneurial Project Award 2015 and the ProSPER.net-Scopus Young Scientist Award, 2014. In addition, more than 14 gene sequences from his fungal researches had been deposited in Gene Bank and nowaday widely used by fungal researchers community. Dr. Siddiquee has established a network of relevant research groups such as institutes, industries and different agencies national and internationally like the Riken Research Institute, Japan, University of Regensburg, Germany and Swiss-German University, Indonesia. In management, he is currently serving some positions including courses coordinator, lab coordinators of biochemistry laboratory and as a member of the academic council at the Biotechnology Research Institute. He is also serving as an editorial member of Fungal Biology and Biotechnology and expert reviewers for several journals, including Biosensors and Bioelectronics, Analytica Chimica Acta, Molecular Biology Reports, etc.,. In pop culture, Dr. Siddiquee findings had been broadcasted in several national (Malaysia) TV stations (TV1, TV2 and TV7) as well as highlighted in various printed news agencies.
RESEARCH INTERESTS
Our research group works at the boundaries of analytical chemistry, physical chemistry, biochemistry, molecular microbiology, molecular genetics and markers, gas chromatography- MAS spectrum, Infrared spectrum and protein purifications. We focus in nanostructuring surfaces to provide them with distinctive functionality. Most frequently we use self-assembled monolayers for modifying the surfaces but also use nanomaterials, such as carbon nanotubes and nanoparticles, and polymers. Biosensors exploit biological recognition molecules, such as enzymes, peptides and DNA, for chemical and biological analysis. We are investigating couple the remarkable specificity of some biological molecules with a signal transducer to allow the detection of a single species.
Phone: +6088-320000 ext. 8467
Address: Biochemistry Lab
Biotechnology Research Institute
Universiti Malaysia Sabah
Jln UMS, 88400 Kota Kinabalu, Sabah, Malaysia
Assoc. Prof. Dr. Md Shafiquzzaman Siddiquee has received his MSc (Molecular Microbiology) and PhD (Bioelectrochemistry) degrees in 2008 and 2010, respectively, from University Putra Malaysia (UPM), Malaysia. Given his achievements, Dr. Siddiquee had received a Best Post Graduate Award, 2011 from UPM for being the most outstanding postgraduate student of the year. Dr. Siddiquee has started his independent academic career as a senior lecturer after that got promotion as an Associate Professor at the Biotechnology Research Institute, University Malaysia Sabah. He is a head of the Biosensor Research Group at the BRI UMS. He has involved in varying types of research areas such as chemo- or/ bio- sensors, and optical sensors based research, production of biofertilizers from oil palm fibres and rice straw, fungal biodiversity, removal of heavy metal ions from sediments and waste water. Currently he has been supervising above 12 post-graduate students (PhD and Master programs) as well as 12 undergraduates and industrial students. He has been published more than 50 papers in high impact journals, 2 books, 8 book chapters and 22 proceeding papers. His findings have yielded, so far, 4 patent, 5 invited speakers and 32 research awards, including notable award of MOHE entrepreneurial Project Award 2015 and the ProSPER.net-Scopus Young Scientist Award, 2014. In addition, more than 14 gene sequences from his fungal researches had been deposited in Gene Bank and nowaday widely used by fungal researchers community. Dr. Siddiquee has established a network of relevant research groups such as institutes, industries and different agencies national and internationally like the Riken Research Institute, Japan, University of Regensburg, Germany and Swiss-German University, Indonesia. In management, he is currently serving some positions including courses coordinator, lab coordinators of biochemistry laboratory and as a member of the academic council at the Biotechnology Research Institute. He is also serving as an editorial member of Fungal Biology and Biotechnology and expert reviewers for several journals, including Biosensors and Bioelectronics, Analytica Chimica Acta, Molecular Biology Reports, etc.,. In pop culture, Dr. Siddiquee findings had been broadcasted in several national (Malaysia) TV stations (TV1, TV2 and TV7) as well as highlighted in various printed news agencies.
RESEARCH INTERESTS
Our research group works at the boundaries of analytical chemistry, physical chemistry, biochemistry, molecular microbiology, molecular genetics and markers, gas chromatography- MAS spectrum, Infrared spectrum and protein purifications. We focus in nanostructuring surfaces to provide them with distinctive functionality. Most frequently we use self-assembled monolayers for modifying the surfaces but also use nanomaterials, such as carbon nanotubes and nanoparticles, and polymers. Biosensors exploit biological recognition molecules, such as enzymes, peptides and DNA, for chemical and biological analysis. We are investigating couple the remarkable specificity of some biological molecules with a signal transducer to allow the detection of a single species.
Phone: +6088-320000 ext. 8467
Address: Biochemistry Lab
Biotechnology Research Institute
Universiti Malaysia Sabah
Jln UMS, 88400 Kota Kinabalu, Sabah, Malaysia
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Books by Shafiquzzaman Siddiquee
This book aims to provide information to undergraduates, post-graduates and researchers studying in fields of Trichoderma. All the topics covered in the book will dealt with sound scientific footing. Chapters related to important research work on applications of Trichoderma will be invited from specialists in the concerned disciplines of International community. Also, all the chapter covered in the book will be derived from current peer reviewed literature as accepted by the international scientific community. It is expected that this will cater to the needs of researchers/academician of science dealing with Trichoderma spp. related research works
Papers by Shafiquzzaman Siddiquee
pesticides used in rice-prawn integrated culture technique. Randomly selected survey
technique was applied through direct observations and personal interviews for this study. :
Data were collected from five different upazillas viz. Fultola and Dumuria of Khulna,
Mollahat, Fokirhat and Chitolmari of Bagerhat district which were situated in the south-west
region in Bangladesh from January to March 2011. Randomly selected survey technique
was applied to collect information from sample farmers which were chosen by consultation
with local Agriculture Extension Officer and Fisheries Officer. A total of 75 (45 from
Bagerhat and 30 from Khulna) sample farmers were asked to mention various information.
Eight active ingredients of pesticides within 28 trade names were used to kill pest such as
the stem borers, green leafhoppers and some of grasshoppers and gall midges infesting
the rice farms. Pesticide frequency was varied between 1 and 3 sprays per crop season. A
total of 94% respondents applied pesticides for controlling pests, 5% respondents used
biological control and 1% respondent did not use any technique for pest management. The pesticide groups Organophosphorus and carbamate were used by 40% and 38% of the
respondents respectively and another 15% farmers used pyrethroid in their rice crop during
the winter period. Various groups of pesticides were used in order of 36% for Carbofuran,
17% for Chloropyriphos, 12% for Malathion, 11% for Diazinon, 7% for Cyhalotrin, 3% for
Carbosulfan, 1% for Thiomax and 7% for Cypermethrin, respectively of the respondent
farmers. No banned pesticides were identified from the respondents during this survey. It is
suggested that pesticides regulation and effective implementation, increasing farmer’s
awareness of effective pesticide use and expansion of IPM (Integrated Pest Management)
practices can be helped towards sustainable integrated rice-prawn culture in the part of
Bangladesh.
cucumber body wall consisted of chondroitin sulfate type backbone with attached sulfated or nonsulfated
fucose side chain. Structurally this compound plays an important role in maintaining the body
wall integrity and possesses a wide spectrum of biological activities. Recently several glycosaminoglycans’
structures have been solved to elucidate its physicochemical activity. The purpose of this review paper
is to elaborate existing structural properties and functions, reporting over 30 years and systematically
discussion herein.
This book aims to provide information to undergraduates, post-graduates and researchers studying in fields of Trichoderma. All the topics covered in the book will dealt with sound scientific footing. Chapters related to important research work on applications of Trichoderma will be invited from specialists in the concerned disciplines of International community. Also, all the chapter covered in the book will be derived from current peer reviewed literature as accepted by the international scientific community. It is expected that this will cater to the needs of researchers/academician of science dealing with Trichoderma spp. related research works
pesticides used in rice-prawn integrated culture technique. Randomly selected survey
technique was applied through direct observations and personal interviews for this study. :
Data were collected from five different upazillas viz. Fultola and Dumuria of Khulna,
Mollahat, Fokirhat and Chitolmari of Bagerhat district which were situated in the south-west
region in Bangladesh from January to March 2011. Randomly selected survey technique
was applied to collect information from sample farmers which were chosen by consultation
with local Agriculture Extension Officer and Fisheries Officer. A total of 75 (45 from
Bagerhat and 30 from Khulna) sample farmers were asked to mention various information.
Eight active ingredients of pesticides within 28 trade names were used to kill pest such as
the stem borers, green leafhoppers and some of grasshoppers and gall midges infesting
the rice farms. Pesticide frequency was varied between 1 and 3 sprays per crop season. A
total of 94% respondents applied pesticides for controlling pests, 5% respondents used
biological control and 1% respondent did not use any technique for pest management. The pesticide groups Organophosphorus and carbamate were used by 40% and 38% of the
respondents respectively and another 15% farmers used pyrethroid in their rice crop during
the winter period. Various groups of pesticides were used in order of 36% for Carbofuran,
17% for Chloropyriphos, 12% for Malathion, 11% for Diazinon, 7% for Cyhalotrin, 3% for
Carbosulfan, 1% for Thiomax and 7% for Cypermethrin, respectively of the respondent
farmers. No banned pesticides were identified from the respondents during this survey. It is
suggested that pesticides regulation and effective implementation, increasing farmer’s
awareness of effective pesticide use and expansion of IPM (Integrated Pest Management)
practices can be helped towards sustainable integrated rice-prawn culture in the part of
Bangladesh.
cucumber body wall consisted of chondroitin sulfate type backbone with attached sulfated or nonsulfated
fucose side chain. Structurally this compound plays an important role in maintaining the body
wall integrity and possesses a wide spectrum of biological activities. Recently several glycosaminoglycans’
structures have been solved to elucidate its physicochemical activity. The purpose of this review paper
is to elaborate existing structural properties and functions, reporting over 30 years and systematically
discussion herein.
disease and increasing yield of production in the agricultural industry. The prevalent soil fungus of Trichoderma
produces lignocellulolytic enzymes that assist the degradation of woody lignocellulose materials. The aim of the
experimental work was to check the potential of lignocellulolytic Trichoderma fungi for the use of rapid composting
of oil palm empty fruit bunches fibers. Fifty-two of Trichoderma isolates from Sabah and seven isolates from
Antarctic were examined for in-vitro lignocellulolytic activity by assaying their ability to develop dark brown pigments,
yellow halo zone, and clear white zone on tannic acid media (TAM) for lignin; Jensen Media (JM) for cellulose; and
modified Melin–Nokrans media (MMNM) for starch. The best six Sabah Trichoderma isolates (5D, 10L2, 10P, 5E,
10X, and 10E2) were found to be potential lignocellulolytic agents based on their diameter of halo zone formed on
amended media for further testing of in vitro bioconversion of oil palm empty fruit bunches. The diameters of halo
zones were measured for the analysis of their ability in degrading lignin, cellulose, and starch. In contrast, Antarctic
Trichoderma isolates consistently exhibited lower lignocellulolytic activities based on their smaller diameter of halo
zone formed on TAM, JM, and MMNM. Most of the Trichoderma isolates are found to synthesize polyphenol oxidase,
endoglucanases, and are able to hydrolyze starch to glucose in the three different media. Thus, the finding shows the
potential of these isolates for use in large-scale composting of oil palm empty fruit bunches.
biological control agent against various plant pathogens. The identification of Trichoderma species worldwide
is currently deduced from micro-morphological descriptions which are tedious and prone to error.
Electrochemical approaches are currently being developed for the detection and analysis of DNA. In the
present study, an electrochemical DNA biosensor was successfully developed based on ionic liquid (e.g.,
1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIM][Otf])), ZnO nanoparticles and a chitosan
(CHIT) nanocomposite membrane on a modified gold electrode (AuE). A single-stranded DNA probe
was immobilized on this electrode. Methylene blue (MB) was used as the hybridization indicator to
monitor the hybridization reaction of the target DNA. Under optimal conditions using differential
pulse voltammetry (DPV), the target DNA sequences were detectable at concentration ranges of
1.0 1018–1.82 104 mol L1, and the detectable limit was 1.0 1019 mol L1. The developed DNA
biosensor enables the study of hybridization with crude DNA fragments and the results of this study
confirm that this DNA biosensor provides a fast, sensitive and convenient way for the species level
identification of Trichoderma harzianum.