RESUMO Avaliou-se a etiologia de bactérias aeróbicas e fúngica e a sensibilidade microbiana in vi... more RESUMO Avaliou-se a etiologia de bactérias aeróbicas e fúngica e a sensibilidade microbiana in vitro de agentes bacterianos, de 207 éguas com endometrite. Obteve-se o isolamento microbiano em 75 (36,5%) amostras de secreção uterina. Isolou-se com maior freqüência Streptococcus beta-hemolítico (32,0%), Escherichia coli (17,3%) e Corynebacterium spp. (12,0%). Dentre os agentes de origem fúngica identificou-se Candida albicans (4,0%) e Geotrichum candidum (1,3%). No teste in vitro de sensibilidade microbiana, a norfloxacina (77,0%), a ampicilina (72,3%) e a gentamicina (63,5%) foram os antimicrobianos mais efetivos. Estreptomicina (68,75%), sulfadiazina/trimetoprim (50,5%) e peni-cilina G (48,7%) apresentaram os maiores índices de resistência frente aos agentes. O presente estudo ratifica a complexidade etiológica na gênese de infecções uterinas na espécie eqüina, e a importância do perfil in vitro de sensibilidade microbiana, como subsídio para procedimentos terapêuticos. PALAVRAS-CHA...
This paper describes the control, epidemiological, pathological, and molecular aspects of an outb... more This paper describes the control, epidemiological, pathological, and molecular aspects of an outbreak of meningoencephalitis in calves due to bovine herpesvirus 5 at a feedlot with 540 animals in São Paulo State, Brazil. The introduction of new animals and contact between the resident animals and the introduced ones were most likely responsible for virus transmission. Bovine herpesvirus 1 vaccine was used, resulting in the efficacy of the outbreak control, although two bovine herpesvirus 1 positive animals, vaccinated and revaccinated, presented meningoencephalitis, thereby characterizing vaccinal failure.
Revista da Sociedade Brasileira de Medicina Tropical, 2015
Toxoplasmosis and leishmaniasis are two worldwide zoonoses caused by the protozoan parasites Toxo... more Toxoplasmosis and leishmaniasis are two worldwide zoonoses caused by the protozoan parasites Toxoplasma gondii and Leishmania spp., respectively. This report describes the clinical and laboratorial findings of a co-infection with both parasites in a 4-year-old female dog suspected of ehrlichiosis that presented anemia, thrombocytopenia, hypoalbuminemia, hyperglobulinemia, tachyzoite-like structures to the lung imprints, and polymerase chain reaction (PCR) results positive for T. gondii (kidney, lung, and liver) and Leishmania spp. Co-infection with Toxoplasma gondii and Leishmania braziliensis was confirmed by sequencing; restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) confirmed an atypical T. gondii genotype circulating in dogs that has been reported to cause human congenital toxoplasmosis.
Introduction: Bovine vaccinia virus (VACV) is a well-known zoonotic agent related to exanthemous ... more Introduction: Bovine vaccinia virus (VACV) is a well-known zoonotic agent related to exanthemous lesions in skin and mucous membranes of dairy cattle and humans, characterized by the formation of vesicles, pustules and ulcers. Mastitis is one of the most common infectious diseases of dairy herds. Bovine mammary infections are caused mainly by bacterial microorganisms, especially staphylococci. To the best of our knowledge, intramammary coinfection with VACV and Staphylococcus aureus in cows has not been reported previously. Case presentation: During an outbreak of exanthematic bovine VACV infection with animals showing vesicles, pustules and haemorrhagic ulcers on the teats, milk samples were collected for mastitis detection. Conclusion: The present report describes a case of intramammary coinfection by VACV and S. aureus in a bovine VACV outbreak.
Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire, 2014
Streptococcus suis is an important pathogen in the swine industry. This study is the first to rep... more Streptococcus suis is an important pathogen in the swine industry. This study is the first to report on the antimicrobial susceptibility of S. suis isolated from clinically healthy pigs in Brazil; the fourth major pork producer in the world. The antimicrobial susceptibility of 260 strains was determined by disc diffusion method. Strains were commonly susceptible to ceftiofur, cephalexin, chloramphenicol, and florfenicol, with more than 80% of the strains being susceptible to these antimicrobials. A high frequency of resistance to some of the antimicrobial agents was demonstrated, with resistance being most common to sulfa-trimethoprim (100%), tetracycline (97.69%), clindamycin (84.61%), norfloxacin (76.92%), and ciprofloxacin (61.15%). A high percentage of multidrug resistant strains (99.61%) were also found. The results of this study indicate that ceftiofur, cephalexin, and florfenicol are the antimicrobials of choice for empirical control of the infections caused by S. suis.
Normal 0 21 MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-na... more Normal 0 21 MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman";} A total of 493 hollander cows were examinated through California Mastitis Test (CMT) in the northern Paraná, for the purpose of studying the occurence of Corynebacterium bovis on subclinical bovine mastitis. Among the 225 milk samples that were positive in the CMT, the C. bovis was isolated in 58 (25,77%). The average microscopic count of somatic cells in milk samples with C. bovis was 1.032.420 cells/ml and the colonies count ware of 912,88 on blood agas (BA) and 879,63 CFU/ml of milk on Plate Count Agar (PCA). Normal 0 21 MicrosoftInternetExplorer4 /* Style Definitions */ tab...
Previously, survival of rabies infection was shown to correlate with low IL-6 serum concentration... more Previously, survival of rabies infection was shown to correlate with low IL-6 serum concentration in mice subjected to post-exposure treatment with the Fuenzalida Palacios rabies vaccine in conjunction with the immunomodulator Propionibacterium acnes, previously Corynebacterium parvum. Considering the substitution of the Fuenzalida Palacios rabies vaccine by the Vero cell raised anti-rabies vaccine in almost all countries, the objective of this
ABSTRACT Objectives: The nucleoside analog ribavirin is one of the antiviral drugs recommended in... more ABSTRACT Objectives: The nucleoside analog ribavirin is one of the antiviral drugs recommended in the management of several viral infections in humans. Although ribavirin has been used in rabies treatment, its efficacy has not been formally proven. The objectives were to study the effect of ribavirin on rabies virus (RABV) infection in neuronal cultures, to analyze whether orally-given ribavirin activates an innate immune response in the brain and whether ribavirin confers protection against brain infection in a model of experimental rabies in mice. Design: The effect of ribavirin on RABV multiplication was evaluated in vitro in both fibroblast (BSRT7) and neuroblastoma cell lines (SK-N-SH) and also in vivo using an experimental rabies post-exposure treatment in C57BL6 mice, which were inoculated with different street RABV strains in different doses.
Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária
This study investigated the etiology of canine ehrlichiosis and possible clinical and epidemiolog... more This study investigated the etiology of canine ehrlichiosis and possible clinical and epidemiological data associated with the infection in 70 dogs suspect of ehrlichiosis attended at the Veterinary Hospital of the São Paulo State University in Botucatu city during 2001 and 2002. Dogs were evaluated by clinical-epidemiological and hematological data and molecular analysis by partial amplification and DNA sequencing of the ehrlichial dsb gene. E. canis DNA was amplified and sequenced in 28 (40.0%) dogs. Dogs younger than 12 months old showed significantly higher infection rates (65.0%; P < 0.05). Diarrhea, apathy, and anorexia were the major clinical signs observed in 55.2% (P = 0.05), 47.0% (P > 0.05), and 42.4% (P > 0.05) of the PCR-positive dogs, respectively. Twenty-five anemic (<5.5 x 10(6) RBC x microL (-1)), and 8 leukopenic (<5.5 x 103 WBC x microL (-1)) dogs were PCR-positive (P > 0.05). All 28 PCR-positive dogs showed thrombocytopenia (< 175 x 103 plate...
The ovine brucellosis caused by Brucella ovis has tropism for reproductive tissues but until now ... more The ovine brucellosis caused by Brucella ovis has tropism for reproductive tissues but until now the mechanism of bacterial persistence is not understood. Cytokine expression profiles were studied for 8 months in rams after being experimentally infected with the rough virulent strain of B. ovis (R-B. ovis) to study the pathogenesis of B. ovis and immune mechanism possibly associated to bacteria tropism and persistence. The messenger RNA (mRNA) expression levels of interleukin-1α (IL-1α), IL-1β, IL-6, IL-10, IL-12, interferon-γ (INF-γ) and tumour necrosis factor-α (TNF-α) cytokines were quantified by real-time quantitative RT-PCR (qRT-PCR) in reproductive tissues (epididymus, testicles, ampolae, vesicular glands and bulbourethral glands), and non-reproductive (liver, spleen and kidneys) tissues at 30, 60, 120 and 240 days post infection (dpi). During the acute phase of infection at 30dpi, the host immune response was most notable demonstrating an up-regulation of several cytokines in...
A new outbreak of Vaccinia virus was observed in Southwest region of São Paulo State, Brazil. The... more A new outbreak of Vaccinia virus was observed in Southwest region of São Paulo State, Brazil. The disease was observed in four small dairy farms with manual milking. Lesions were detected in cattle and in humans previously vaccinated and not vaccinated against smallpox. Although several reports of Vaccinia virus outbreaks have been occurring in Brazil, it was not yet reported
RESUMO Avaliou-se a etiologia de bactérias aeróbicas e fúngica e a sensibilidade microbiana in vi... more RESUMO Avaliou-se a etiologia de bactérias aeróbicas e fúngica e a sensibilidade microbiana in vitro de agentes bacterianos, de 207 éguas com endometrite. Obteve-se o isolamento microbiano em 75 (36,5%) amostras de secreção uterina. Isolou-se com maior freqüência Streptococcus beta-hemolítico (32,0%), Escherichia coli (17,3%) e Corynebacterium spp. (12,0%). Dentre os agentes de origem fúngica identificou-se Candida albicans (4,0%) e Geotrichum candidum (1,3%). No teste in vitro de sensibilidade microbiana, a norfloxacina (77,0%), a ampicilina (72,3%) e a gentamicina (63,5%) foram os antimicrobianos mais efetivos. Estreptomicina (68,75%), sulfadiazina/trimetoprim (50,5%) e peni-cilina G (48,7%) apresentaram os maiores índices de resistência frente aos agentes. O presente estudo ratifica a complexidade etiológica na gênese de infecções uterinas na espécie eqüina, e a importância do perfil in vitro de sensibilidade microbiana, como subsídio para procedimentos terapêuticos. PALAVRAS-CHA...
This paper describes the control, epidemiological, pathological, and molecular aspects of an outb... more This paper describes the control, epidemiological, pathological, and molecular aspects of an outbreak of meningoencephalitis in calves due to bovine herpesvirus 5 at a feedlot with 540 animals in São Paulo State, Brazil. The introduction of new animals and contact between the resident animals and the introduced ones were most likely responsible for virus transmission. Bovine herpesvirus 1 vaccine was used, resulting in the efficacy of the outbreak control, although two bovine herpesvirus 1 positive animals, vaccinated and revaccinated, presented meningoencephalitis, thereby characterizing vaccinal failure.
Revista da Sociedade Brasileira de Medicina Tropical, 2015
Toxoplasmosis and leishmaniasis are two worldwide zoonoses caused by the protozoan parasites Toxo... more Toxoplasmosis and leishmaniasis are two worldwide zoonoses caused by the protozoan parasites Toxoplasma gondii and Leishmania spp., respectively. This report describes the clinical and laboratorial findings of a co-infection with both parasites in a 4-year-old female dog suspected of ehrlichiosis that presented anemia, thrombocytopenia, hypoalbuminemia, hyperglobulinemia, tachyzoite-like structures to the lung imprints, and polymerase chain reaction (PCR) results positive for T. gondii (kidney, lung, and liver) and Leishmania spp. Co-infection with Toxoplasma gondii and Leishmania braziliensis was confirmed by sequencing; restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) confirmed an atypical T. gondii genotype circulating in dogs that has been reported to cause human congenital toxoplasmosis.
Introduction: Bovine vaccinia virus (VACV) is a well-known zoonotic agent related to exanthemous ... more Introduction: Bovine vaccinia virus (VACV) is a well-known zoonotic agent related to exanthemous lesions in skin and mucous membranes of dairy cattle and humans, characterized by the formation of vesicles, pustules and ulcers. Mastitis is one of the most common infectious diseases of dairy herds. Bovine mammary infections are caused mainly by bacterial microorganisms, especially staphylococci. To the best of our knowledge, intramammary coinfection with VACV and Staphylococcus aureus in cows has not been reported previously. Case presentation: During an outbreak of exanthematic bovine VACV infection with animals showing vesicles, pustules and haemorrhagic ulcers on the teats, milk samples were collected for mastitis detection. Conclusion: The present report describes a case of intramammary coinfection by VACV and S. aureus in a bovine VACV outbreak.
Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire, 2014
Streptococcus suis is an important pathogen in the swine industry. This study is the first to rep... more Streptococcus suis is an important pathogen in the swine industry. This study is the first to report on the antimicrobial susceptibility of S. suis isolated from clinically healthy pigs in Brazil; the fourth major pork producer in the world. The antimicrobial susceptibility of 260 strains was determined by disc diffusion method. Strains were commonly susceptible to ceftiofur, cephalexin, chloramphenicol, and florfenicol, with more than 80% of the strains being susceptible to these antimicrobials. A high frequency of resistance to some of the antimicrobial agents was demonstrated, with resistance being most common to sulfa-trimethoprim (100%), tetracycline (97.69%), clindamycin (84.61%), norfloxacin (76.92%), and ciprofloxacin (61.15%). A high percentage of multidrug resistant strains (99.61%) were also found. The results of this study indicate that ceftiofur, cephalexin, and florfenicol are the antimicrobials of choice for empirical control of the infections caused by S. suis.
Normal 0 21 MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-na... more Normal 0 21 MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tabela normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman";} A total of 493 hollander cows were examinated through California Mastitis Test (CMT) in the northern Paraná, for the purpose of studying the occurence of Corynebacterium bovis on subclinical bovine mastitis. Among the 225 milk samples that were positive in the CMT, the C. bovis was isolated in 58 (25,77%). The average microscopic count of somatic cells in milk samples with C. bovis was 1.032.420 cells/ml and the colonies count ware of 912,88 on blood agas (BA) and 879,63 CFU/ml of milk on Plate Count Agar (PCA). Normal 0 21 MicrosoftInternetExplorer4 /* Style Definitions */ tab...
Previously, survival of rabies infection was shown to correlate with low IL-6 serum concentration... more Previously, survival of rabies infection was shown to correlate with low IL-6 serum concentration in mice subjected to post-exposure treatment with the Fuenzalida Palacios rabies vaccine in conjunction with the immunomodulator Propionibacterium acnes, previously Corynebacterium parvum. Considering the substitution of the Fuenzalida Palacios rabies vaccine by the Vero cell raised anti-rabies vaccine in almost all countries, the objective of this
ABSTRACT Objectives: The nucleoside analog ribavirin is one of the antiviral drugs recommended in... more ABSTRACT Objectives: The nucleoside analog ribavirin is one of the antiviral drugs recommended in the management of several viral infections in humans. Although ribavirin has been used in rabies treatment, its efficacy has not been formally proven. The objectives were to study the effect of ribavirin on rabies virus (RABV) infection in neuronal cultures, to analyze whether orally-given ribavirin activates an innate immune response in the brain and whether ribavirin confers protection against brain infection in a model of experimental rabies in mice. Design: The effect of ribavirin on RABV multiplication was evaluated in vitro in both fibroblast (BSRT7) and neuroblastoma cell lines (SK-N-SH) and also in vivo using an experimental rabies post-exposure treatment in C57BL6 mice, which were inoculated with different street RABV strains in different doses.
Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária
This study investigated the etiology of canine ehrlichiosis and possible clinical and epidemiolog... more This study investigated the etiology of canine ehrlichiosis and possible clinical and epidemiological data associated with the infection in 70 dogs suspect of ehrlichiosis attended at the Veterinary Hospital of the São Paulo State University in Botucatu city during 2001 and 2002. Dogs were evaluated by clinical-epidemiological and hematological data and molecular analysis by partial amplification and DNA sequencing of the ehrlichial dsb gene. E. canis DNA was amplified and sequenced in 28 (40.0%) dogs. Dogs younger than 12 months old showed significantly higher infection rates (65.0%; P < 0.05). Diarrhea, apathy, and anorexia were the major clinical signs observed in 55.2% (P = 0.05), 47.0% (P > 0.05), and 42.4% (P > 0.05) of the PCR-positive dogs, respectively. Twenty-five anemic (<5.5 x 10(6) RBC x microL (-1)), and 8 leukopenic (<5.5 x 103 WBC x microL (-1)) dogs were PCR-positive (P > 0.05). All 28 PCR-positive dogs showed thrombocytopenia (< 175 x 103 plate...
The ovine brucellosis caused by Brucella ovis has tropism for reproductive tissues but until now ... more The ovine brucellosis caused by Brucella ovis has tropism for reproductive tissues but until now the mechanism of bacterial persistence is not understood. Cytokine expression profiles were studied for 8 months in rams after being experimentally infected with the rough virulent strain of B. ovis (R-B. ovis) to study the pathogenesis of B. ovis and immune mechanism possibly associated to bacteria tropism and persistence. The messenger RNA (mRNA) expression levels of interleukin-1α (IL-1α), IL-1β, IL-6, IL-10, IL-12, interferon-γ (INF-γ) and tumour necrosis factor-α (TNF-α) cytokines were quantified by real-time quantitative RT-PCR (qRT-PCR) in reproductive tissues (epididymus, testicles, ampolae, vesicular glands and bulbourethral glands), and non-reproductive (liver, spleen and kidneys) tissues at 30, 60, 120 and 240 days post infection (dpi). During the acute phase of infection at 30dpi, the host immune response was most notable demonstrating an up-regulation of several cytokines in...
A new outbreak of Vaccinia virus was observed in Southwest region of São Paulo State, Brazil. The... more A new outbreak of Vaccinia virus was observed in Southwest region of São Paulo State, Brazil. The disease was observed in four small dairy farms with manual milking. Lesions were detected in cattle and in humans previously vaccinated and not vaccinated against smallpox. Although several reports of Vaccinia virus outbreaks have been occurring in Brazil, it was not yet reported
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