This document presents the mission and strategy of the<strong> Italian Competence Centre on... more This document presents the mission and strategy of the<strong> Italian Competence Centre on Open Science, FAIR, and EOSC</strong>. The Competence Centre is an initiative born within the Italian Computing and Data Infrastructure (ICDI), a forum created by representatives of major Italian Research Infrastructures and e-Infrastructures, with the aim of promoting sinergies at the national level, and optimising the Italian participation to European and global challenges in this field, including the European Open Science Cloud (<strong>EOSC</strong>), the European Data Infrastructure (<strong>EDI</strong>) and <strong>HPC</strong>. This working paper depicts the mission and objectives of the ICDI Competence Centre, a network of experts with various skills and competences that are supporting the national stakeholders on topics related to Open Science, FAIR principles application and participation to the EOSC. The different actors and roles ar...
The bergamot polyphenolic fraction (BPF) was evaluated in the F1FO-ATPase activity of swine heart... more The bergamot polyphenolic fraction (BPF) was evaluated in the F1FO-ATPase activity of swine heart mitochondria. In the presence of a concentration higher than 50 µg/mL BPF, the ATPase activity of F1FO-ATPase, dependent on the natural cofactor Mg2+, increased by 15%, whereas the enzyme activity in the presence of Ca2+ was inhibited by 10%. By considering this opposite BPF effect, the F1FO-ATPase activity involved in providing ATP synthesis in oxidative phosphorylation and triggering mitochondrial permeability transition pore (mPTP) formation has been evaluated. The BPF improved the catalytic coupling of oxidative phosphorylation in the presence of a substrate at the first phosphorylation site, boosting the respiratory control ratios (state 3/state 4) by 25% and 85% with 50 µg/mL and 100 µg/mL BPF, respectively. Conversely, the substrate at the second phosphorylation site led to the improvement of the state 3/state 4 ratios by 15% only with 100 µg/mL BPF. Moreover, the BPF carried out...
The foetal bovine serum (FBS) concentration could influence functional parameters of IPEC-J2 cell... more The foetal bovine serum (FBS) concentration could influence functional parameters of IPEC-J2 cells. IPEC-J2 is a non-transformed continuous epithelial cell line that represents an established in vitro model to study porcine gut inflammation and alterations of intestinal integrity. This cell line also represents a good translational model thanks to the high similitudes between pig and human gastrointestinal tract. With the aim to assess if the FBS-dependent functional variations are linked to the bioenergetic aspects, the addition of 5% and 10% FBS in the IPEC-J2 culture medium were tested. Doubling time and TEER measurement indicated that cells cultured at higher FBS dose grow faster and as a more compact monolayer. 10% FBS increases ATP production and mitochondrial oxidative phosphorylation (OxPhos) and does not affect glycolysis. Both at 5% and 10% FBS ATP production mainly comes from OxPhos and FBS concentration does not affect the cell respiration bioenergetic parameters. Noteworthy, IPEC-J2 treated with 5% and 10% FBS have a metabolic potential since both OxPhos and glycolysis increase by > 100% and < 50%, respectively in comparison with baseline metabolism. Moreover, glucose, fatty acids and glutamine constitute the preferred metabolic fuel for mitochondrial respiration at both FBS conditions tested. Accordingly, the cells flexibility to oxidize these substrates shows that IPEC-J2 mitochondria cannot maintain the basal ATP production without oxidizing all the substrates available irrespective of FBS concentration. To sum up, in IPEC-J2 cells OxPhos increases with the FBS-stimulated functional physiological parameters to fulfil ATP requirements.
This study was conducted to evaluate the safety and the beneficial effects of dietary supplementa... more This study was conducted to evaluate the safety and the beneficial effects of dietary supplementation with Boswellia serrata (Bs) and Salix alba (Sa) in Leghorn hens during the critical pre-laying and laying phases. A total of 120 pullets, 17 weeks of age, were assigned to two groups (Control—C; Treated—T, n = 60 each). For 12 weeks, the T group received a diet supplemented with 0.3% of dry extracts of Bs (5%) and Sa (5%). The study lasted 19 weeks. Productive performance, serum analytes, H/L ratio, IgA and anti-IBV antibodies were investigated. Water intake was significantly higher, while body and egg weight was significantly lower for the T group (p < 0.05). No other differences were detected in performance parameters, serum analytes, IgA and H/L ratio excluding t0, with a significantly (p < 0.05) higher H/R ratio and higher titers of anti-IBV antibody for the T group. Overall, the data obtained in this study show that the supplementation with Bs and Sa was safe and resulted...
Differential expression of nitric oxide synthases in porcine aortic endothelial cells during LPS-... more Differential expression of nitric oxide synthases in porcine aortic endothelial cells during LPS-induced apoptosis
Stem-cell therapy provides a promising strategy for patients with ischemic heart disease. In rece... more Stem-cell therapy provides a promising strategy for patients with ischemic heart disease. In recent years, numerous studies related to this therapeutic approach were performed; however, the results were often heterogeneous and contradictory. For this reason, we conducted a systematic review and meta-analysis of trials, reporting the use of stem-cell treatment against acute or chronic ischemic cardiomyopathies in large animal models with regard to Left Ventricular Ejection Fraction (LVEF). The defined research strategy was applied to the PubMed database to identify relevant studies published from January 2011 to July 2021. A random-effect meta-analysis was performed on LVEF mean data at follow-up between control and stem-cell-treated animals. In order to improve the definition of the effect measure and to analyze the factors that could influence the outcomes, a subgroup comparison was conducted. Sixty-six studies (n = 1183 animals) satisfied our inclusion criteria. Ischemia/reperfusi...
Several reliable methods to produce transgenic animals utilize the male genome. After penetration... more Several reliable methods to produce transgenic animals utilize the male genome. After penetration into oocyte, sperm DNA undergoes dramatic conformational changes that could represent a great opportunity for exogenous DNA to be integrated in the zygote genome. Among the enzymes responsible for sperm remodeling, a nuclease could be involved. The presence of a DNase I in oocytes has not been much investigated. To date, an immunolocalization of DNase I has been reported only in rat immature oocytes and the presence of nuclease activities has been shown in avian oocytes. The present study was conducted to verify whether a DNase-I like activity is present in MII mature pig oocytes. To do this, oocyte extracts were assessed for nuclease activity by a plasmid degradation assay and by zymography; these analyses evi-denced a 33 kDa, Ca21/Mg21 dependent DNase I-like activity that was inhibited by Zn21. A further identification of DNase I was achieved by Western blot, immunofluorescence and RT...
Prostaglandin F2-alpha receptor (FPr) expression on porcine corpus luteum microvascular endotheli... more Prostaglandin F2-alpha receptor (FPr) expression on porcine corpus luteum microvascular endothelial cells (pCL-MVECs)
Aim: The aim of the study was to set up a porcine ex vivo model of acid-induced damage and to eva... more Aim: The aim of the study was to set up a porcine ex vivo model of acid-induced damage and to evaluate its performance by means of multichannel intraluminal impedance and pH (MII-pH) live recording, histology, and Evans blue (EB) permeability assay. Materials and Methods: Thirteen esophagi, collected at a slaughterhouse, were ablated of their sphincters, pinned upright on a support, and placed in a thermostatic hood at 37°C with two infusion tubes and an MII-pH probe inserted in the top end. Three esophagi (histology controls) were only left in the hood for 3.5 h before sampling, while the remaining organs underwent the experimental protocol including saline infusion and recovery recording, and acid solution infusion and recovery recording. Results: MII-pH analysis highlighted a significantly stronger decrease during acid infusion when compared to saline, but a better post-infusion recovery for saline solution. At the end of the protocol, MII was still statistically lower than basel...
Abstract Effetto benefico dell&#x27;inalazione di monossido di carbonio durante lo shock endo... more Abstract Effetto benefico dell&#x27;inalazione di monossido di carbonio durante lo shock endotossico del suino/SM Mazzola, M. Costanzi, M. Albertini, M. Forni, F. Pirrone, CV Pastore, ML Bacci, MG Clement.-2006. Meeting SO. FI. VET: Ossido d&#x27;azoto e monossido ...
INTRODUCTION Semen quality assessment represents a fundamental step for obtaining successful Arti... more INTRODUCTION Semen quality assessment represents a fundamental step for obtaining successful Artificial Insemination (AI) in pig industries, however the decline in boar fertility, not related to apparent causes, is a common and economically relevant problem. In commercial settings, ejaculates were evaluated at collection, but traditional quality estimates are not able to foretell fertility outcome. New fertility parameters have been therefore studied in vitro and compared with traditional ones. This study was designed to evaluate if temporary fertility decline could be due to contact with pathogens at level not sufficient to evocate clinical signs of disease but adequate to stimulate the seroconversion.
Simple Summary The roe deer is a small wild ruminant, very common in Europe and Asia; adult speci... more Simple Summary The roe deer is a small wild ruminant, very common in Europe and Asia; adult specimens are sexually active only during summer, in very short timeframes. Peculiarly, males, also known as bucks, produce spermatozoa only in this period, with a subsequent morph-functional testicular involution. In seasonal breeders, melatonin plays a pivotal role by converting light information and controlling the testicular hormonal function and, recently, its local production within testes has been described in other species. The aim of the present work was to study testicular melatonin and its synthesis pathway in roe deer during the pre-rut (June–July) and post-rut (August–September) periods, and correlate it with morph-functional testicular changes. Samples were opportunistically obtained from hunted specimens according to the local hunting calendar. The results also seem to suggest a local melatonin production in this species, but no correlations with testicular involution parameter...
Purpose AL2106 is a new medical device based on a mixture of chondroitin sulphate in a xyloglucan... more Purpose AL2106 is a new medical device based on a mixture of chondroitin sulphate in a xyloglucan and glycerol solution made to maximize its bioadhesive capability to the esophageal mucosa. The aim of the present study was twofold to evaluate the AL2106 protective effect on the esophageal mucosa when exposed to an acidic solution mimicking gastric reflux and to assess the resilience of this effect to saline washing. Materials and Methods A porcine ex vivo model was used and the effects of the new medical device were compared to a sodium alginate suspension (SAS) already present on the market which was assumed as reference. Mucosal damage was induced in 19 porcine esophagi by perfusion with an acidic solution added with pepsin, and Evans blue dye (EBD) tissue uptake was used as an indicator of mucosal permeability. The EBD penetration, expressed as EBD µg/g of dry tissue, was assessed in specimens of untreated damaged mucosa and in specimens treated with AL2106 or SAS. The same evalu...
This document presents the mission and strategy of the<strong> Italian Competence Centre on... more This document presents the mission and strategy of the<strong> Italian Competence Centre on Open Science, FAIR, and EOSC</strong>. The Competence Centre is an initiative born within the Italian Computing and Data Infrastructure (ICDI), a forum created by representatives of major Italian Research Infrastructures and e-Infrastructures, with the aim of promoting sinergies at the national level, and optimising the Italian participation to European and global challenges in this field, including the European Open Science Cloud (<strong>EOSC</strong>), the European Data Infrastructure (<strong>EDI</strong>) and <strong>HPC</strong>. This working paper depicts the mission and objectives of the ICDI Competence Centre, a network of experts with various skills and competences that are supporting the national stakeholders on topics related to Open Science, FAIR principles application and participation to the EOSC. The different actors and roles ar...
The bergamot polyphenolic fraction (BPF) was evaluated in the F1FO-ATPase activity of swine heart... more The bergamot polyphenolic fraction (BPF) was evaluated in the F1FO-ATPase activity of swine heart mitochondria. In the presence of a concentration higher than 50 µg/mL BPF, the ATPase activity of F1FO-ATPase, dependent on the natural cofactor Mg2+, increased by 15%, whereas the enzyme activity in the presence of Ca2+ was inhibited by 10%. By considering this opposite BPF effect, the F1FO-ATPase activity involved in providing ATP synthesis in oxidative phosphorylation and triggering mitochondrial permeability transition pore (mPTP) formation has been evaluated. The BPF improved the catalytic coupling of oxidative phosphorylation in the presence of a substrate at the first phosphorylation site, boosting the respiratory control ratios (state 3/state 4) by 25% and 85% with 50 µg/mL and 100 µg/mL BPF, respectively. Conversely, the substrate at the second phosphorylation site led to the improvement of the state 3/state 4 ratios by 15% only with 100 µg/mL BPF. Moreover, the BPF carried out...
The foetal bovine serum (FBS) concentration could influence functional parameters of IPEC-J2 cell... more The foetal bovine serum (FBS) concentration could influence functional parameters of IPEC-J2 cells. IPEC-J2 is a non-transformed continuous epithelial cell line that represents an established in vitro model to study porcine gut inflammation and alterations of intestinal integrity. This cell line also represents a good translational model thanks to the high similitudes between pig and human gastrointestinal tract. With the aim to assess if the FBS-dependent functional variations are linked to the bioenergetic aspects, the addition of 5% and 10% FBS in the IPEC-J2 culture medium were tested. Doubling time and TEER measurement indicated that cells cultured at higher FBS dose grow faster and as a more compact monolayer. 10% FBS increases ATP production and mitochondrial oxidative phosphorylation (OxPhos) and does not affect glycolysis. Both at 5% and 10% FBS ATP production mainly comes from OxPhos and FBS concentration does not affect the cell respiration bioenergetic parameters. Noteworthy, IPEC-J2 treated with 5% and 10% FBS have a metabolic potential since both OxPhos and glycolysis increase by > 100% and < 50%, respectively in comparison with baseline metabolism. Moreover, glucose, fatty acids and glutamine constitute the preferred metabolic fuel for mitochondrial respiration at both FBS conditions tested. Accordingly, the cells flexibility to oxidize these substrates shows that IPEC-J2 mitochondria cannot maintain the basal ATP production without oxidizing all the substrates available irrespective of FBS concentration. To sum up, in IPEC-J2 cells OxPhos increases with the FBS-stimulated functional physiological parameters to fulfil ATP requirements.
This study was conducted to evaluate the safety and the beneficial effects of dietary supplementa... more This study was conducted to evaluate the safety and the beneficial effects of dietary supplementation with Boswellia serrata (Bs) and Salix alba (Sa) in Leghorn hens during the critical pre-laying and laying phases. A total of 120 pullets, 17 weeks of age, were assigned to two groups (Control—C; Treated—T, n = 60 each). For 12 weeks, the T group received a diet supplemented with 0.3% of dry extracts of Bs (5%) and Sa (5%). The study lasted 19 weeks. Productive performance, serum analytes, H/L ratio, IgA and anti-IBV antibodies were investigated. Water intake was significantly higher, while body and egg weight was significantly lower for the T group (p < 0.05). No other differences were detected in performance parameters, serum analytes, IgA and H/L ratio excluding t0, with a significantly (p < 0.05) higher H/R ratio and higher titers of anti-IBV antibody for the T group. Overall, the data obtained in this study show that the supplementation with Bs and Sa was safe and resulted...
Differential expression of nitric oxide synthases in porcine aortic endothelial cells during LPS-... more Differential expression of nitric oxide synthases in porcine aortic endothelial cells during LPS-induced apoptosis
Stem-cell therapy provides a promising strategy for patients with ischemic heart disease. In rece... more Stem-cell therapy provides a promising strategy for patients with ischemic heart disease. In recent years, numerous studies related to this therapeutic approach were performed; however, the results were often heterogeneous and contradictory. For this reason, we conducted a systematic review and meta-analysis of trials, reporting the use of stem-cell treatment against acute or chronic ischemic cardiomyopathies in large animal models with regard to Left Ventricular Ejection Fraction (LVEF). The defined research strategy was applied to the PubMed database to identify relevant studies published from January 2011 to July 2021. A random-effect meta-analysis was performed on LVEF mean data at follow-up between control and stem-cell-treated animals. In order to improve the definition of the effect measure and to analyze the factors that could influence the outcomes, a subgroup comparison was conducted. Sixty-six studies (n = 1183 animals) satisfied our inclusion criteria. Ischemia/reperfusi...
Several reliable methods to produce transgenic animals utilize the male genome. After penetration... more Several reliable methods to produce transgenic animals utilize the male genome. After penetration into oocyte, sperm DNA undergoes dramatic conformational changes that could represent a great opportunity for exogenous DNA to be integrated in the zygote genome. Among the enzymes responsible for sperm remodeling, a nuclease could be involved. The presence of a DNase I in oocytes has not been much investigated. To date, an immunolocalization of DNase I has been reported only in rat immature oocytes and the presence of nuclease activities has been shown in avian oocytes. The present study was conducted to verify whether a DNase-I like activity is present in MII mature pig oocytes. To do this, oocyte extracts were assessed for nuclease activity by a plasmid degradation assay and by zymography; these analyses evi-denced a 33 kDa, Ca21/Mg21 dependent DNase I-like activity that was inhibited by Zn21. A further identification of DNase I was achieved by Western blot, immunofluorescence and RT...
Prostaglandin F2-alpha receptor (FPr) expression on porcine corpus luteum microvascular endotheli... more Prostaglandin F2-alpha receptor (FPr) expression on porcine corpus luteum microvascular endothelial cells (pCL-MVECs)
Aim: The aim of the study was to set up a porcine ex vivo model of acid-induced damage and to eva... more Aim: The aim of the study was to set up a porcine ex vivo model of acid-induced damage and to evaluate its performance by means of multichannel intraluminal impedance and pH (MII-pH) live recording, histology, and Evans blue (EB) permeability assay. Materials and Methods: Thirteen esophagi, collected at a slaughterhouse, were ablated of their sphincters, pinned upright on a support, and placed in a thermostatic hood at 37°C with two infusion tubes and an MII-pH probe inserted in the top end. Three esophagi (histology controls) were only left in the hood for 3.5 h before sampling, while the remaining organs underwent the experimental protocol including saline infusion and recovery recording, and acid solution infusion and recovery recording. Results: MII-pH analysis highlighted a significantly stronger decrease during acid infusion when compared to saline, but a better post-infusion recovery for saline solution. At the end of the protocol, MII was still statistically lower than basel...
Abstract Effetto benefico dell&#x27;inalazione di monossido di carbonio durante lo shock endo... more Abstract Effetto benefico dell&#x27;inalazione di monossido di carbonio durante lo shock endotossico del suino/SM Mazzola, M. Costanzi, M. Albertini, M. Forni, F. Pirrone, CV Pastore, ML Bacci, MG Clement.-2006. Meeting SO. FI. VET: Ossido d&#x27;azoto e monossido ...
INTRODUCTION Semen quality assessment represents a fundamental step for obtaining successful Arti... more INTRODUCTION Semen quality assessment represents a fundamental step for obtaining successful Artificial Insemination (AI) in pig industries, however the decline in boar fertility, not related to apparent causes, is a common and economically relevant problem. In commercial settings, ejaculates were evaluated at collection, but traditional quality estimates are not able to foretell fertility outcome. New fertility parameters have been therefore studied in vitro and compared with traditional ones. This study was designed to evaluate if temporary fertility decline could be due to contact with pathogens at level not sufficient to evocate clinical signs of disease but adequate to stimulate the seroconversion.
Simple Summary The roe deer is a small wild ruminant, very common in Europe and Asia; adult speci... more Simple Summary The roe deer is a small wild ruminant, very common in Europe and Asia; adult specimens are sexually active only during summer, in very short timeframes. Peculiarly, males, also known as bucks, produce spermatozoa only in this period, with a subsequent morph-functional testicular involution. In seasonal breeders, melatonin plays a pivotal role by converting light information and controlling the testicular hormonal function and, recently, its local production within testes has been described in other species. The aim of the present work was to study testicular melatonin and its synthesis pathway in roe deer during the pre-rut (June–July) and post-rut (August–September) periods, and correlate it with morph-functional testicular changes. Samples were opportunistically obtained from hunted specimens according to the local hunting calendar. The results also seem to suggest a local melatonin production in this species, but no correlations with testicular involution parameter...
Purpose AL2106 is a new medical device based on a mixture of chondroitin sulphate in a xyloglucan... more Purpose AL2106 is a new medical device based on a mixture of chondroitin sulphate in a xyloglucan and glycerol solution made to maximize its bioadhesive capability to the esophageal mucosa. The aim of the present study was twofold to evaluate the AL2106 protective effect on the esophageal mucosa when exposed to an acidic solution mimicking gastric reflux and to assess the resilience of this effect to saline washing. Materials and Methods A porcine ex vivo model was used and the effects of the new medical device were compared to a sodium alginate suspension (SAS) already present on the market which was assumed as reference. Mucosal damage was induced in 19 porcine esophagi by perfusion with an acidic solution added with pepsin, and Evans blue dye (EBD) tissue uptake was used as an indicator of mucosal permeability. The EBD penetration, expressed as EBD µg/g of dry tissue, was assessed in specimens of untreated damaged mucosa and in specimens treated with AL2106 or SAS. The same evalu...
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