El presente libro, resultado de estudios etnobotónicos realizados por investigadores de la Univer... more El presente libro, resultado de estudios etnobotónicos realizados por investigadores de la Universidad de Ciencias y Artes de Cliiapas y de lo Universidad Nocional Autónoma de México, recupera la tradición oral que sobre los plantas medicinales tienen los zoques de las comunidades de Copainalá, Rayón, Tapalapa y Tapilula. Documenta también los nombres en zoque de los especies y contribuye a la elaboración de la memoria escrita sobre el uso y manejo que los pobladores hacen de los recursos naturales de su entorno. Mediante revisiones bibliogróficas, aporta datos sobre los usos y nombres no locales y los propiedades medicinales de los plantas; es uno versión corregida y aumentada de la publicación Plantas usadas por los zoques en enfermedades gastrointestinales y respiratorias que se distribuyó en Rayón y Tapalapa, y que fue editado por la Universidad de Ciencias y Artes de Chiapos y el Sistema de Investigación Benito Juórez en 1998. ISBN: 17a-t07-6S10-ll-l
Resumen es: Los ceparios o colecciones de microorganismos son fuentes de recursos geneticos cuyo ... more Resumen es: Los ceparios o colecciones de microorganismos son fuentes de recursos geneticos cuyo proposito es la preservacion de la diversidad biologica, garantizand...
Data collected about the profile of E. coli strains (virulence genes, phylogrouping, antibiotics ... more Data collected about the profile of E. coli strains (virulence genes, phylogrouping, antibiotics profile) isolated from 240 captive Iguana iguana of Chiapas, Mexico.
Introduction The direct Gram stain of a urethral discharge from males with urethritis is a rapid ... more Introduction The direct Gram stain of a urethral discharge from males with urethritis is a rapid and sensitive screening test for Neisseria gonorrhoeae infection. This image shows gram-negative diplococci within polymorphonuclear leukocytes. Cocci are 0.6 to 1.0 mm in diameter and can occur singly, but are more often found in pairs with adjacent sides flattened (diplococci). Bacterial culture and carbohydrate fermentation studies provide definitive identification of the organism. N. gonorrhoeae is the etiological agent of the sexually transmitted disease gonorrhea. Infections caused by this organism are localized to the mucosal surfaces in the area of initial exposure to the organism (e.g., cervix, conjunctiva, pharyngeal surface, anorectal area, or urethra of males). The organism may also be spread from infected mother to newborn during birth. Methods This image is of a Gram stain of urethral discharge from a symptomatic male with urethritis. A urethral discharge was collected by...
Summary These images show various stages in the development of Ascaris lumbricoides, including an... more Summary These images show various stages in the development of Ascaris lumbricoides, including an unfertilized egg, a fertilized egg, an infective egg containing a larva, and a larva hatching from an infective egg. Introduction Figure 1 shows an unfertilized egg of Ascaris lumbricoides containing a mass of refractile granules. It has an average length of 90 μm. Figure 2 depicts a fertilized egg. It appears broad and oval in shape with a thick shell that is stained brown by bile. Figures 3 and 4 show an infective egg of A. lumbricoides containing a larva and a larva of A. lumbricoides hatching from an infective egg, respectively. After ingestion, the larvae penetrate the intestinal mucosa where they are subsequently transported by blood circulation to the liver, heart, lungs, and trachea. From the trachea, the larvae are transported to the epiglottis where they are then swallowed (1). When swallowed, they pass down into the small intestine where they develop into adults (Fig. 5, an ...
Introduction The image shows a direct wet mount prepared from a white vaginal discharge showing p... more Introduction The image shows a direct wet mount prepared from a white vaginal discharge showing pseudohyphae, budding yeast, and human epithelial cells. Examination of a fresh vaginal discharge provides the simplest and most rapid diagnostic test in aiding presumptive identification of Candida species. Methods The vaginal discharge was collected from a 37-year-old female with dysuria and vulvovaginal pruritus. The sample was diluted with equal parts of physiologic sterile saline and applied directly to the surface of a slide. A coverslip was placed over the surface of the sample, and excess liquid was blotted from around the edges. References 1. Buxton, R. 2007. Examination of Gram stains of vaginal secretions. ASM Microbe Library. 2. Casasone, A., F. De Berrnardis, and G. Santoni. 2007. Anticandidal immunity and vaginitis: novel opportunities for immune intervention. Infec. Immun. 75:4675–4686. 3. Gilligan, P. H., M. L. Smiley, and D. S. Shapiro. 1997. Cases in medical mi...
Summary These images show two laboratory tools for diagnosis of enteroaggregative Escherichia col... more Summary These images show two laboratory tools for diagnosis of enteroaggregative Escherichia coli (EAEC). The adherence assay, which assesses the ability of EAEC cells to adhere in a honeycomb pattern to both the glass coverslip and to eukaryotic cultured cells, and the polymerase chain reaction technique in which hallmark genes of EAEC are amplified. Introduction Enteroaggregative Escherichia coli has been recognized as an etiologic agent of acute and persistent diarrhea among children, travelers, and in human immunodeficiency virus-infected persons (7). This enteric bacterium can be diagnosed by means of the adhesion assay, considered as the "gold standard" (3, 6), but the test is expensive and is a time and labor intensive method. A specific, sensitive, and less time-consuming technique is the multiplex polymerase chain reaction (mPCR), in which two or more sets of primer pairs specific for different targets are introduced in the same tube (11, 12). Several authors h...
La Internet es una de las tecnologias de informacion y comunicacion (TICs) que permite el interca... more La Internet es una de las tecnologias de informacion y comunicacion (TICs) que permite el intercambio de informacion mediante un sistema de documentos que conducen a otros textos relacionados y enlazados entre si, este tipo de tecnologia esta impactando en todas las actividades de la vida cotidiana del ser humano, siendo particularmente notoria en la educacion. La Biologia es una ciencia sujeta de estas vertientes novedosas y los materiales didactico-interactivos que se encuentran en esta red virtual, son recursos poderosos para la ensenanza y el aprendizaje de esta ciencia. Sin embargo, la localizacion de los recursos es dificil sobre todo por el desconocimiento de estos sitios virtuales ademas de la aparicion constante de nuevas paginas. El proposito de este trabajo es dar a conocer una compilacion de direcciones electronicas cuyo contenido didactico apoye el proceso de ensenanza aprendizaje de la Biologia
Introduction The figure shows a typical Hymenolepis nana egg found in feces from a 2-year-old chi... more Introduction The figure shows a typical Hymenolepis nana egg found in feces from a 2-year-old child with diarrhea. An oncosphere containing the hexacanth embryo with three pairs of hooks and two polar thickenings can be observed. The polar filaments are present in the space between the oncosphere and the eggshell; those filaments are difficult to see in this picture, however. Methods The feces were collected in a sterile wide-mouth plastic bottle and prepared using the formalin-ethyl acetate sedimentation procedure; the sediment was analyzed using light microscopy. The egg was photographed with a digital camera (Leica) using an oil immersion objective (1,000x). Discussion H. nana , known as the dwarf tapeworm, is the smallest of the adult human tapeworms. It is normally a parasite of mice, in which the life cycle involves various beetles as intermediate hosts. In humans, transmission is usually accomplished by direct ingestion of infective eggs containing oncospheres. Also humans ...
Introduction The figure shows an Enterobius vermicularis (pinworm) egg found in a feces sample. ... more Introduction The figure shows an Enterobius vermicularis (pinworm) egg found in a feces sample. G enerally the ovum measures 50 to 60 μm long by 20 to 40 μm wide. The figure is of an embryonated ovum, flattened on one side, with a thin colorless shell. Methods Diagnosis of pinworm infection is usually based on the recovery of typical eggs by means of the transparent cellophane ("Scotch") tape preparation. With tape held between the thumb and forefingers of both hands, the outward -facing adhesive surface is pressed against the skin across the anal opening with even thorough pressure. The tape is then transferred to a glass microscope slide with the adhesive surface downward in such a way as to firmly adhere to the slide. Because adult female pinworms live in the lower portion of the intestinal tract, females often lay their eggs on the surface of the fecal mass or on the anal skin folds. As a result, the eggs are not well mixed within the feces and usually will not be det...
RESUMEN El juego de Jeopardy! es un concurso televisivo en el que se hacen preguntas a los partic... more RESUMEN El juego de Jeopardy! es un concurso televisivo en el que se hacen preguntas a los participantes sobre diversos temas de cultura general. herramienta didáctica denominada Jeopardy bioquímico basada en el juego Microbial Jeopardy!™ con algunas modificaciones. Para crear la presentación se usó el programa informático Power Point (Microsoft Office, 2007) y como ejemplo se usaron tres tópicos de bioquímica: ácidos nucleicos, proteínas y ADN recombinante. ABSTRACT The Jeopardy! game is a television quiz show in which questions about general knowledge are presented to contestants; they must answer in question form. This game has been used with educational purposes and scientific literature shows evidence about their didactic benefits. The purpose of this work was to show how to construct a ludic didactic tool named The biochemistry Jeopardy, based on the Microbial Jeo-pardy! ™ game with some modifications. A series of slides were created by means of the Power Point software (Micro...
Los helmintos son los gusanos parasitarios causantes de las infecciones más comunes que afectan a... more Los helmintos son los gusanos parasitarios causantes de las infecciones más comunes que afectan al ser humano y otros animales. Se encuentran en grandes concentraciones en aguas residuales y lodos (restos de estas aguas) que, a su vez, son utilizados en la agricultura, por los nutrientes que aportan al suelo, tales como nitrógeno y fósforo. Una vía de transmisión de huevos de helminto (HH) es el agua, ya que dichos huevos son liberados en las heces de las personas que han sido infectadas, y así llegan a las aguas residuales, convirtiéndose en un problema sanitario, debido a su gran capacidad para sobrevivir por largos periodos, pues son extremadamente resistentes a los tratamientos de desinfección del agua. Tal resistencia está dada por la composición química de las capas de estos huevos, principalmente, formadas por lípidos y proteínas.
RESUMENIntroducción. El parásito Trypanosoma cruzi es el agente etiológico de la enfermedad de Ch... more RESUMENIntroducción. El parásito Trypanosoma cruzi es el agente etiológico de la enfermedad de Chagas. Su transmisión es a través de insectos de la subfamilia Triatominae, donde Triatoma dimidiata es uno de los vectores principales en México y Centro América. La transmisión del parásito depende de factores como la tasa de infección de las distintas especies y el tiempo de defecación después de la alimentación. Objetivo. Evaluar la infección con T. cruzi en los cinco estadios de T. dimidiata y determinar el tiempo de defecación postprandial. Material y métodos. Treinta individuos de cada estadio de T. dimidiata fueron alimentados con sangre infectada con T. cruzi. Se llevó a cabo una segunda alimentación 20 días posteriores para evaluar la presencia del parásito en las heces y medir el tiempo de defecación postprandial. Resultados. El número total de ejemplares infectados con T. cruzi fue de 99 (66 %). No se encontraron diferencias significativas en la tasa de infección por estadio n...
The Journal of Infection in Developing Countries, 2018
Introduction: Among beta-hemolytic streptococci, Streptococcus pyogenes causes a wide variety of ... more Introduction: Among beta-hemolytic streptococci, Streptococcus pyogenes causes a wide variety of human disease including pharyngitis, necrotizing fasciitis and streptococcal toxic syndrome. Group A Streptococcus (GAS) uses a variety of virulence traits to colonize and then cause damage to the host; others species of beta-hemolytic streptococci are considered as emerging pathogens for humans. Despite its recognized virulence, only few studies have investigated virulence factors of GAS strains isolated in Mexico. Methodology: We conducted an epidemiological study to investigate the prevalence of GAS strains in child illnesses in Chiapas Mexico. Virulence genes encoding proteases, DNases, superantigens, as well as susceptibility to antibiotics were investigated. Results: During 2010, 2013 and 2014, beta-hemolytic streptococci (N=12) were isolated from cases of bacterial infections including pharyngitis and bacteremia, with a prevalence of 0.42, 0.04 and 0.20%, respectively. S. pyogenes...
El presente libro, resultado de estudios etnobotónicos realizados por investigadores de la Univer... more El presente libro, resultado de estudios etnobotónicos realizados por investigadores de la Universidad de Ciencias y Artes de Cliiapas y de lo Universidad Nocional Autónoma de México, recupera la tradición oral que sobre los plantas medicinales tienen los zoques de las comunidades de Copainalá, Rayón, Tapalapa y Tapilula. Documenta también los nombres en zoque de los especies y contribuye a la elaboración de la memoria escrita sobre el uso y manejo que los pobladores hacen de los recursos naturales de su entorno. Mediante revisiones bibliogróficas, aporta datos sobre los usos y nombres no locales y los propiedades medicinales de los plantas; es uno versión corregida y aumentada de la publicación Plantas usadas por los zoques en enfermedades gastrointestinales y respiratorias que se distribuyó en Rayón y Tapalapa, y que fue editado por la Universidad de Ciencias y Artes de Chiapos y el Sistema de Investigación Benito Juórez en 1998. ISBN: 17a-t07-6S10-ll-l
Resumen es: Los ceparios o colecciones de microorganismos son fuentes de recursos geneticos cuyo ... more Resumen es: Los ceparios o colecciones de microorganismos son fuentes de recursos geneticos cuyo proposito es la preservacion de la diversidad biologica, garantizand...
Data collected about the profile of E. coli strains (virulence genes, phylogrouping, antibiotics ... more Data collected about the profile of E. coli strains (virulence genes, phylogrouping, antibiotics profile) isolated from 240 captive Iguana iguana of Chiapas, Mexico.
Introduction The direct Gram stain of a urethral discharge from males with urethritis is a rapid ... more Introduction The direct Gram stain of a urethral discharge from males with urethritis is a rapid and sensitive screening test for Neisseria gonorrhoeae infection. This image shows gram-negative diplococci within polymorphonuclear leukocytes. Cocci are 0.6 to 1.0 mm in diameter and can occur singly, but are more often found in pairs with adjacent sides flattened (diplococci). Bacterial culture and carbohydrate fermentation studies provide definitive identification of the organism. N. gonorrhoeae is the etiological agent of the sexually transmitted disease gonorrhea. Infections caused by this organism are localized to the mucosal surfaces in the area of initial exposure to the organism (e.g., cervix, conjunctiva, pharyngeal surface, anorectal area, or urethra of males). The organism may also be spread from infected mother to newborn during birth. Methods This image is of a Gram stain of urethral discharge from a symptomatic male with urethritis. A urethral discharge was collected by...
Summary These images show various stages in the development of Ascaris lumbricoides, including an... more Summary These images show various stages in the development of Ascaris lumbricoides, including an unfertilized egg, a fertilized egg, an infective egg containing a larva, and a larva hatching from an infective egg. Introduction Figure 1 shows an unfertilized egg of Ascaris lumbricoides containing a mass of refractile granules. It has an average length of 90 μm. Figure 2 depicts a fertilized egg. It appears broad and oval in shape with a thick shell that is stained brown by bile. Figures 3 and 4 show an infective egg of A. lumbricoides containing a larva and a larva of A. lumbricoides hatching from an infective egg, respectively. After ingestion, the larvae penetrate the intestinal mucosa where they are subsequently transported by blood circulation to the liver, heart, lungs, and trachea. From the trachea, the larvae are transported to the epiglottis where they are then swallowed (1). When swallowed, they pass down into the small intestine where they develop into adults (Fig. 5, an ...
Introduction The image shows a direct wet mount prepared from a white vaginal discharge showing p... more Introduction The image shows a direct wet mount prepared from a white vaginal discharge showing pseudohyphae, budding yeast, and human epithelial cells. Examination of a fresh vaginal discharge provides the simplest and most rapid diagnostic test in aiding presumptive identification of Candida species. Methods The vaginal discharge was collected from a 37-year-old female with dysuria and vulvovaginal pruritus. The sample was diluted with equal parts of physiologic sterile saline and applied directly to the surface of a slide. A coverslip was placed over the surface of the sample, and excess liquid was blotted from around the edges. References 1. Buxton, R. 2007. Examination of Gram stains of vaginal secretions. ASM Microbe Library. 2. Casasone, A., F. De Berrnardis, and G. Santoni. 2007. Anticandidal immunity and vaginitis: novel opportunities for immune intervention. Infec. Immun. 75:4675–4686. 3. Gilligan, P. H., M. L. Smiley, and D. S. Shapiro. 1997. Cases in medical mi...
Summary These images show two laboratory tools for diagnosis of enteroaggregative Escherichia col... more Summary These images show two laboratory tools for diagnosis of enteroaggregative Escherichia coli (EAEC). The adherence assay, which assesses the ability of EAEC cells to adhere in a honeycomb pattern to both the glass coverslip and to eukaryotic cultured cells, and the polymerase chain reaction technique in which hallmark genes of EAEC are amplified. Introduction Enteroaggregative Escherichia coli has been recognized as an etiologic agent of acute and persistent diarrhea among children, travelers, and in human immunodeficiency virus-infected persons (7). This enteric bacterium can be diagnosed by means of the adhesion assay, considered as the "gold standard" (3, 6), but the test is expensive and is a time and labor intensive method. A specific, sensitive, and less time-consuming technique is the multiplex polymerase chain reaction (mPCR), in which two or more sets of primer pairs specific for different targets are introduced in the same tube (11, 12). Several authors h...
La Internet es una de las tecnologias de informacion y comunicacion (TICs) que permite el interca... more La Internet es una de las tecnologias de informacion y comunicacion (TICs) que permite el intercambio de informacion mediante un sistema de documentos que conducen a otros textos relacionados y enlazados entre si, este tipo de tecnologia esta impactando en todas las actividades de la vida cotidiana del ser humano, siendo particularmente notoria en la educacion. La Biologia es una ciencia sujeta de estas vertientes novedosas y los materiales didactico-interactivos que se encuentran en esta red virtual, son recursos poderosos para la ensenanza y el aprendizaje de esta ciencia. Sin embargo, la localizacion de los recursos es dificil sobre todo por el desconocimiento de estos sitios virtuales ademas de la aparicion constante de nuevas paginas. El proposito de este trabajo es dar a conocer una compilacion de direcciones electronicas cuyo contenido didactico apoye el proceso de ensenanza aprendizaje de la Biologia
Introduction The figure shows a typical Hymenolepis nana egg found in feces from a 2-year-old chi... more Introduction The figure shows a typical Hymenolepis nana egg found in feces from a 2-year-old child with diarrhea. An oncosphere containing the hexacanth embryo with three pairs of hooks and two polar thickenings can be observed. The polar filaments are present in the space between the oncosphere and the eggshell; those filaments are difficult to see in this picture, however. Methods The feces were collected in a sterile wide-mouth plastic bottle and prepared using the formalin-ethyl acetate sedimentation procedure; the sediment was analyzed using light microscopy. The egg was photographed with a digital camera (Leica) using an oil immersion objective (1,000x). Discussion H. nana , known as the dwarf tapeworm, is the smallest of the adult human tapeworms. It is normally a parasite of mice, in which the life cycle involves various beetles as intermediate hosts. In humans, transmission is usually accomplished by direct ingestion of infective eggs containing oncospheres. Also humans ...
Introduction The figure shows an Enterobius vermicularis (pinworm) egg found in a feces sample. ... more Introduction The figure shows an Enterobius vermicularis (pinworm) egg found in a feces sample. G enerally the ovum measures 50 to 60 μm long by 20 to 40 μm wide. The figure is of an embryonated ovum, flattened on one side, with a thin colorless shell. Methods Diagnosis of pinworm infection is usually based on the recovery of typical eggs by means of the transparent cellophane ("Scotch") tape preparation. With tape held between the thumb and forefingers of both hands, the outward -facing adhesive surface is pressed against the skin across the anal opening with even thorough pressure. The tape is then transferred to a glass microscope slide with the adhesive surface downward in such a way as to firmly adhere to the slide. Because adult female pinworms live in the lower portion of the intestinal tract, females often lay their eggs on the surface of the fecal mass or on the anal skin folds. As a result, the eggs are not well mixed within the feces and usually will not be det...
RESUMEN El juego de Jeopardy! es un concurso televisivo en el que se hacen preguntas a los partic... more RESUMEN El juego de Jeopardy! es un concurso televisivo en el que se hacen preguntas a los participantes sobre diversos temas de cultura general. herramienta didáctica denominada Jeopardy bioquímico basada en el juego Microbial Jeopardy!™ con algunas modificaciones. Para crear la presentación se usó el programa informático Power Point (Microsoft Office, 2007) y como ejemplo se usaron tres tópicos de bioquímica: ácidos nucleicos, proteínas y ADN recombinante. ABSTRACT The Jeopardy! game is a television quiz show in which questions about general knowledge are presented to contestants; they must answer in question form. This game has been used with educational purposes and scientific literature shows evidence about their didactic benefits. The purpose of this work was to show how to construct a ludic didactic tool named The biochemistry Jeopardy, based on the Microbial Jeo-pardy! ™ game with some modifications. A series of slides were created by means of the Power Point software (Micro...
Los helmintos son los gusanos parasitarios causantes de las infecciones más comunes que afectan a... more Los helmintos son los gusanos parasitarios causantes de las infecciones más comunes que afectan al ser humano y otros animales. Se encuentran en grandes concentraciones en aguas residuales y lodos (restos de estas aguas) que, a su vez, son utilizados en la agricultura, por los nutrientes que aportan al suelo, tales como nitrógeno y fósforo. Una vía de transmisión de huevos de helminto (HH) es el agua, ya que dichos huevos son liberados en las heces de las personas que han sido infectadas, y así llegan a las aguas residuales, convirtiéndose en un problema sanitario, debido a su gran capacidad para sobrevivir por largos periodos, pues son extremadamente resistentes a los tratamientos de desinfección del agua. Tal resistencia está dada por la composición química de las capas de estos huevos, principalmente, formadas por lípidos y proteínas.
RESUMENIntroducción. El parásito Trypanosoma cruzi es el agente etiológico de la enfermedad de Ch... more RESUMENIntroducción. El parásito Trypanosoma cruzi es el agente etiológico de la enfermedad de Chagas. Su transmisión es a través de insectos de la subfamilia Triatominae, donde Triatoma dimidiata es uno de los vectores principales en México y Centro América. La transmisión del parásito depende de factores como la tasa de infección de las distintas especies y el tiempo de defecación después de la alimentación. Objetivo. Evaluar la infección con T. cruzi en los cinco estadios de T. dimidiata y determinar el tiempo de defecación postprandial. Material y métodos. Treinta individuos de cada estadio de T. dimidiata fueron alimentados con sangre infectada con T. cruzi. Se llevó a cabo una segunda alimentación 20 días posteriores para evaluar la presencia del parásito en las heces y medir el tiempo de defecación postprandial. Resultados. El número total de ejemplares infectados con T. cruzi fue de 99 (66 %). No se encontraron diferencias significativas en la tasa de infección por estadio n...
The Journal of Infection in Developing Countries, 2018
Introduction: Among beta-hemolytic streptococci, Streptococcus pyogenes causes a wide variety of ... more Introduction: Among beta-hemolytic streptococci, Streptococcus pyogenes causes a wide variety of human disease including pharyngitis, necrotizing fasciitis and streptococcal toxic syndrome. Group A Streptococcus (GAS) uses a variety of virulence traits to colonize and then cause damage to the host; others species of beta-hemolytic streptococci are considered as emerging pathogens for humans. Despite its recognized virulence, only few studies have investigated virulence factors of GAS strains isolated in Mexico. Methodology: We conducted an epidemiological study to investigate the prevalence of GAS strains in child illnesses in Chiapas Mexico. Virulence genes encoding proteases, DNases, superantigens, as well as susceptibility to antibiotics were investigated. Results: During 2010, 2013 and 2014, beta-hemolytic streptococci (N=12) were isolated from cases of bacterial infections including pharyngitis and bacteremia, with a prevalence of 0.42, 0.04 and 0.20%, respectively. S. pyogenes...
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Papers by Javier Gutierrez Jimenez