In vitro, and possibly in vivo, hemoglobin polymerization and red blood cell sickling appear to b... more In vitro, and possibly in vivo, hemoglobin polymerization and red blood cell sickling appear to be widespread in codfish. In this article, we show that the hemoglobins of the two Arctic fish Lycodes reticulatus and Gadus morhua also have the tendency to polymerize, as monitored by dynamic light scattering experiments. The elucidation of the primary structure of the single hemoglobin of the zoarcid L. reticulatus shows the presence of a large number of cysteyl residues in α and β chains. Their role in eliciting the ability to produce polymers was also addressed by MALDI-TOF and TOF-TOF mass spectrometry. The G.morhua globins are also rich in Cys, but unlike in L. reticulatus, polymerization does not seem to be disulfide driven. The widespread occurrence of the polymerization phenomenon displayed by hemoglobins of Arctic fish supports the hypothesis that this feature may bea response to stressful environmental conditions.
Acta Crystallographica Section C Crystal Structure Communications, 2002
In the title compound, C(27)H(39)IN(3)(+).I(-), the acridinium system shows the usual approximate... more In the title compound, C(27)H(39)IN(3)(+).I(-), the acridinium system shows the usual approximate mirror symmetry about the central C.N line, and the corresponding bond lengths and angles in the two halves agree within experimental error. The alkyl chain at the ring N atom is initially perpendicular to the ring plane and then bends sharply at the fourth C atom. Pairs of centrosymmetrically related cations overlap two of their rings and the dimethylamino groups are also partly involved in the overlap. Each I(-) ion is involved in short-range interactions with two cations. These interactions give rise to a 14-membered cyclic structure, which involves pairs of cations and anions across an inversion centre.
Complexes of 2-(2-pyridinylmethyl)amino-3-(2-pyridinylmethylene)amino-2-butenedinitrile, (PPH), h... more Complexes of 2-(2-pyridinylmethyl)amino-3-(2-pyridinylmethylene)amino-2-butenedinitrile, (PPH), have been prepared with Fe(II), Co(II) and Ni(II). The complexes have the general formula [M{(PP)2H}]PF6·2H2O. Two dimorphs of the Fe(II) complex have been obtained, and single crystal x-ray structures of them have been completed. The molecular structures in the orthorhombic and monoclinic forms are closely related.
Acta Crystallographica Section B Structural Crystallography and Crystal Chemistry, 1977
L-Cysteinyl-L-cysteine crystallizes in the orthorhombic space group P2,2,2, with a = 8.629 (7), b... more L-Cysteinyl-L-cysteine crystallizes in the orthorhombic space group P2,2,2, with a = 8.629 (7), b = 23.500 (9), c = 5.145 (2) A. The structure, solved by the heavy-atom method, was refined by least-squares calculations to an R of 0-042, from 963 observed reflexions. The ...
Acta Crystallographica Section C Crystal Structure Communications, 1983
... A Novel Conformation of the L-Lysine Side Chain. BY SANTE CAPASSO, CARLO A. MATTIA, LELIO MAZ... more ... A Novel Conformation of the L-Lysine Side Chain. BY SANTE CAPASSO, CARLO A. MATTIA, LELIO MAZZARELLA AND ADRIANA ZAGARI Istituto Chimico, Universitd di Napoli, Via Mezzocannone 4, Napoli, Italy. (Received 27 July 1982; accepted 4 October 1982). Abstract. ...
Journal of the Chemical Society, Perkin Transactions 2, 1980
The aerial oxidation of L-cysteinyl-L-cysteine in water at pH 8.5 has been investigated. The reac... more The aerial oxidation of L-cysteinyl-L-cysteine in water at pH 8.5 has been investigated. The reaction products, monomeric cyclic disulphide and parallel and antiparallel cyclic dimers, were fractionated by gel and thin layer chromatography. The relative yields of the products are independent ...
Sso7d is a 62-residue protein from the hyperthemophilic archaeon Sulfolobus solfataricus with a d... more Sso7d is a 62-residue protein from the hyperthemophilic archaeon Sulfolobus solfataricus with a denaturation temperature close to 100 degrees C around neutral pH. An engineered form of Sso7d truncated at leucine 54 (L54Delta) is significantly less stable, with a denaturation temperature of 53 degrees C. Molecular dynamics (MD) studies of Sso7d and its truncated form at two different temperatures have been performed. The results of the MD simulations at 300 K indicate that: (1) the flexibility of Sso7d chain at 300 K agrees with that detected from X-ray and NMR structural studies; (2) L54Delta remains stable in the native folded conformation and possesses an overall dynamic behavior similar to that of the parent protein. MD simulations performed at 500 K, 10 ns long, indicate that, while Sso7d is in-silico resistant to high temperature, the truncated variant partially unfolds, revealing the early phases of the thermal unfolding pathway of the protein. Analysis of the trajectories of ...
International journal of peptide and protein research, 1995
The kinetics of the spontaneous formation of 2,5-dioxopiperazines from peptides containing the Ti... more The kinetics of the spontaneous formation of 2,5-dioxopiperazines from peptides containing the Tic (1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) residue in the 2-position of the sequence has been studied in DMSO and water solution. The reaction is first order in Tic-peptide and subject to general-acid catalysis. Moreover, only the fraction of peptide having the amino terminal group in the deprotonated state reacts with appreciable rate. In pure organic solvent, and in aqueous solution with low buffer concentration, the degradation reaction of Tic-peptides is very low; at 20 degrees C for the peptide H-Tyr-Tic-Phe-Phe-NH2, in DMSO and in neutral water in the absence of buffer, the half-lives (t1/2) are 3 x 10(4) and 1.2 x 10(4) h, respectively. The addition of carboxylic acids or buffers to the reaction solutions markedly increases the reaction rate; in 0.01 m HAc in DMSO and in 0.1 M phosphate buffer in water, pH 7.1, t1/2 values for the tetrapeptide are 61 and 121 h, respectiv...
A novel bromo-compound, C 11 H 10 N 5 O 2 Br, has been isolated from the sponges Axinella verruco... more A novel bromo-compound, C 11 H 10 N 5 O 2 Br, has been isolated from the sponges Axinella verrucosa and Acanthella aurantiaca. The structure was determined as 2 on special grounds and by X-ray analysis. ... EE Garcia, LE Benjamin and R. Ian Fryer JCS Chem. Comm. ...
The aminosuccinyl (Asu) residue is formed as intermediate in some peptide and protein reactions. ... more The aminosuccinyl (Asu) residue is formed as intermediate in some peptide and protein reactions. Potential energy calculations, using the parameters of the empirical conformational energy program for peptides (ECEPP), were performed on blocked X-Asu dipeptides, where X = Gly, Ala, Ser, Val and Pro. Results indicate that intra-residue interactions are dominant, and the low-energy dipeptide conformations correspond to the low-energy single-residue minima. Comparisons were made with previous results from energy calculations on blocked Asu-X dipeptides (X = Gly, Ala, Ser and Val).
ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
An NAD + -dependent alcohol dehydrogenase from the extreme thermophilic archaebacterium Sulfolobu... more An NAD + -dependent alcohol dehydrogenase from the extreme thermophilic archaebacterium Sulfolobus solfataricus has been crystallized in the holo-enzyme and apo-enzyme forms. Crystals of the holo-enzyme grow from 2-methyl-2,4-pentanediol at pH 8·4 with the addition of ...
Journal of the Chemical Society, Dalton Transactions, 1974
By Sante Capasso, Federico Giordano, Carlo Mattia, Lelio Mazzarella, and Alberto Ripamonti, lsti... more By Sante Capasso, Federico Giordano, Carlo Mattia, Lelio Mazzarella, and Alberto Ripamonti, lstituto Chimico, Universitil degli Studi, via Mezzocannone 4,801 34 Napoli, Italy ... Crystals of bis(pyridoxy1idene-DL-valinato) nickel(l1). (I), are orthorhombic, space group ...
Journal of the Chemical Society, Perkin Transactions 2, 1993
... Sante Capasso,B Lelio Mazzarella,e Filomena Sica,B Adriana Zagari and Severo Salvadori a Depa... more ... Sante Capasso,B Lelio Mazzarella,e Filomena Sica,B Adriana Zagari and Severo Salvadori a Department of Chemistry, via Mezzocannone, 4, 80 734 Napoli, Italy Department of Pharmaceutical Sciences ... 7 P. Galletti, P. D. Ingrosso, C. Manna, F. Sica, S. Capasso, P. Pucci ...
In vitro, and possibly in vivo, hemoglobin polymerization and red blood cell sickling appear to b... more In vitro, and possibly in vivo, hemoglobin polymerization and red blood cell sickling appear to be widespread in codfish. In this article, we show that the hemoglobins of the two Arctic fish Lycodes reticulatus and Gadus morhua also have the tendency to polymerize, as monitored by dynamic light scattering experiments. The elucidation of the primary structure of the single hemoglobin of the zoarcid L. reticulatus shows the presence of a large number of cysteyl residues in α and β chains. Their role in eliciting the ability to produce polymers was also addressed by MALDI-TOF and TOF-TOF mass spectrometry. The G.morhua globins are also rich in Cys, but unlike in L. reticulatus, polymerization does not seem to be disulfide driven. The widespread occurrence of the polymerization phenomenon displayed by hemoglobins of Arctic fish supports the hypothesis that this feature may bea response to stressful environmental conditions.
Acta Crystallographica Section C Crystal Structure Communications, 2002
In the title compound, C(27)H(39)IN(3)(+).I(-), the acridinium system shows the usual approximate... more In the title compound, C(27)H(39)IN(3)(+).I(-), the acridinium system shows the usual approximate mirror symmetry about the central C.N line, and the corresponding bond lengths and angles in the two halves agree within experimental error. The alkyl chain at the ring N atom is initially perpendicular to the ring plane and then bends sharply at the fourth C atom. Pairs of centrosymmetrically related cations overlap two of their rings and the dimethylamino groups are also partly involved in the overlap. Each I(-) ion is involved in short-range interactions with two cations. These interactions give rise to a 14-membered cyclic structure, which involves pairs of cations and anions across an inversion centre.
Complexes of 2-(2-pyridinylmethyl)amino-3-(2-pyridinylmethylene)amino-2-butenedinitrile, (PPH), h... more Complexes of 2-(2-pyridinylmethyl)amino-3-(2-pyridinylmethylene)amino-2-butenedinitrile, (PPH), have been prepared with Fe(II), Co(II) and Ni(II). The complexes have the general formula [M{(PP)2H}]PF6·2H2O. Two dimorphs of the Fe(II) complex have been obtained, and single crystal x-ray structures of them have been completed. The molecular structures in the orthorhombic and monoclinic forms are closely related.
Acta Crystallographica Section B Structural Crystallography and Crystal Chemistry, 1977
L-Cysteinyl-L-cysteine crystallizes in the orthorhombic space group P2,2,2, with a = 8.629 (7), b... more L-Cysteinyl-L-cysteine crystallizes in the orthorhombic space group P2,2,2, with a = 8.629 (7), b = 23.500 (9), c = 5.145 (2) A. The structure, solved by the heavy-atom method, was refined by least-squares calculations to an R of 0-042, from 963 observed reflexions. The ...
Acta Crystallographica Section C Crystal Structure Communications, 1983
... A Novel Conformation of the L-Lysine Side Chain. BY SANTE CAPASSO, CARLO A. MATTIA, LELIO MAZ... more ... A Novel Conformation of the L-Lysine Side Chain. BY SANTE CAPASSO, CARLO A. MATTIA, LELIO MAZZARELLA AND ADRIANA ZAGARI Istituto Chimico, Universitd di Napoli, Via Mezzocannone 4, Napoli, Italy. (Received 27 July 1982; accepted 4 October 1982). Abstract. ...
Journal of the Chemical Society, Perkin Transactions 2, 1980
The aerial oxidation of L-cysteinyl-L-cysteine in water at pH 8.5 has been investigated. The reac... more The aerial oxidation of L-cysteinyl-L-cysteine in water at pH 8.5 has been investigated. The reaction products, monomeric cyclic disulphide and parallel and antiparallel cyclic dimers, were fractionated by gel and thin layer chromatography. The relative yields of the products are independent ...
Sso7d is a 62-residue protein from the hyperthemophilic archaeon Sulfolobus solfataricus with a d... more Sso7d is a 62-residue protein from the hyperthemophilic archaeon Sulfolobus solfataricus with a denaturation temperature close to 100 degrees C around neutral pH. An engineered form of Sso7d truncated at leucine 54 (L54Delta) is significantly less stable, with a denaturation temperature of 53 degrees C. Molecular dynamics (MD) studies of Sso7d and its truncated form at two different temperatures have been performed. The results of the MD simulations at 300 K indicate that: (1) the flexibility of Sso7d chain at 300 K agrees with that detected from X-ray and NMR structural studies; (2) L54Delta remains stable in the native folded conformation and possesses an overall dynamic behavior similar to that of the parent protein. MD simulations performed at 500 K, 10 ns long, indicate that, while Sso7d is in-silico resistant to high temperature, the truncated variant partially unfolds, revealing the early phases of the thermal unfolding pathway of the protein. Analysis of the trajectories of ...
International journal of peptide and protein research, 1995
The kinetics of the spontaneous formation of 2,5-dioxopiperazines from peptides containing the Ti... more The kinetics of the spontaneous formation of 2,5-dioxopiperazines from peptides containing the Tic (1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) residue in the 2-position of the sequence has been studied in DMSO and water solution. The reaction is first order in Tic-peptide and subject to general-acid catalysis. Moreover, only the fraction of peptide having the amino terminal group in the deprotonated state reacts with appreciable rate. In pure organic solvent, and in aqueous solution with low buffer concentration, the degradation reaction of Tic-peptides is very low; at 20 degrees C for the peptide H-Tyr-Tic-Phe-Phe-NH2, in DMSO and in neutral water in the absence of buffer, the half-lives (t1/2) are 3 x 10(4) and 1.2 x 10(4) h, respectively. The addition of carboxylic acids or buffers to the reaction solutions markedly increases the reaction rate; in 0.01 m HAc in DMSO and in 0.1 M phosphate buffer in water, pH 7.1, t1/2 values for the tetrapeptide are 61 and 121 h, respectiv...
A novel bromo-compound, C 11 H 10 N 5 O 2 Br, has been isolated from the sponges Axinella verruco... more A novel bromo-compound, C 11 H 10 N 5 O 2 Br, has been isolated from the sponges Axinella verrucosa and Acanthella aurantiaca. The structure was determined as 2 on special grounds and by X-ray analysis. ... EE Garcia, LE Benjamin and R. Ian Fryer JCS Chem. Comm. ...
The aminosuccinyl (Asu) residue is formed as intermediate in some peptide and protein reactions. ... more The aminosuccinyl (Asu) residue is formed as intermediate in some peptide and protein reactions. Potential energy calculations, using the parameters of the empirical conformational energy program for peptides (ECEPP), were performed on blocked X-Asu dipeptides, where X = Gly, Ala, Ser, Val and Pro. Results indicate that intra-residue interactions are dominant, and the low-energy dipeptide conformations correspond to the low-energy single-residue minima. Comparisons were made with previous results from energy calculations on blocked Asu-X dipeptides (X = Gly, Ala, Ser and Val).
ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
An NAD + -dependent alcohol dehydrogenase from the extreme thermophilic archaebacterium Sulfolobu... more An NAD + -dependent alcohol dehydrogenase from the extreme thermophilic archaebacterium Sulfolobus solfataricus has been crystallized in the holo-enzyme and apo-enzyme forms. Crystals of the holo-enzyme grow from 2-methyl-2,4-pentanediol at pH 8·4 with the addition of ...
Journal of the Chemical Society, Dalton Transactions, 1974
By Sante Capasso, Federico Giordano, Carlo Mattia, Lelio Mazzarella, and Alberto Ripamonti, lsti... more By Sante Capasso, Federico Giordano, Carlo Mattia, Lelio Mazzarella, and Alberto Ripamonti, lstituto Chimico, Universitil degli Studi, via Mezzocannone 4,801 34 Napoli, Italy ... Crystals of bis(pyridoxy1idene-DL-valinato) nickel(l1). (I), are orthorhombic, space group ...
Journal of the Chemical Society, Perkin Transactions 2, 1993
... Sante Capasso,B Lelio Mazzarella,e Filomena Sica,B Adriana Zagari and Severo Salvadori a Depa... more ... Sante Capasso,B Lelio Mazzarella,e Filomena Sica,B Adriana Zagari and Severo Salvadori a Department of Chemistry, via Mezzocannone, 4, 80 734 Napoli, Italy Department of Pharmaceutical Sciences ... 7 P. Galletti, P. D. Ingrosso, C. Manna, F. Sica, S. Capasso, P. Pucci ...
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