Biology

Objective: Benzene (BZ) is an important pollutant compound, present in both occupational and general environment that has been linked to adverse health effect. The aim of the current study was to investigate the curative and preventive effect of Atriplex halimus L. (Ah) extract against BZ-induced nephrotoxicity in rats. Methods: A total of 30 male albino Wistar rats were divided into five groups (n=6): Control, Ah, BZ, AhP+BZ, and BZ+AhC. BZ (100 mg/kg b.w) was added in their drinking water for 15 weeks. Ah (Aqueous Extract of aerial parts) was given by gavage during the past 30 days of BZ-exposed for curative treatment (AhC) and all the duration of BZ exposure in the animals for preventive treatment (AhP). Some biochemical, oxidative stress parameters, and histopathology of kidney tissue were studied. Results: Obtained results revealed that BZ exposure to rats caused a significant elevation in urea, creatinine, and malondialdehyde levels. Then, it led to reducing the glutathione le...

Objective: The present study was designed to estimate the preventive and curative potency of aqueous extract of Atriplex halimus L. (Ah) aerial parts against benzene (BZ) intoxication in liver rats. Methods: 30 male albino rats were divided into five groups of 6 rats each: Control, Ah, BZ, AhP+BZ, and BZ+AhC. BZ (100 mg/kg b.w) was added in drinking water for 15 weeks. Aqueous extract of Atriplex halimus was received intragastrically during the last 30 days of BZ exposure for curative treatment (AhC) and all the duration of BZ exposure for preventive treatment (AhP). At the end the experiment, body weight gain and relative liver weight were estimated and liver enzyme markers (AST, ALT, ALP and LDH) were analysed by spectrophotometry. Histopathological studies on hepatic tissue were also performed by the method of Hematoxylin and Eosin staining. Results: Benzene administrated to rats caused an alteration in physiological parameters (body and organs weigh) and hepatic enzymes markers (AST, ALT, ALP and LDH). In addition, Histopathological studies showed a massive degeneration in liver tissue in BZ-exposed rats. However, treatment by Atriplex halimus especially preventive effect ameliorated most of the adverse effects induced by benzene. Ah restored the altered of physiological, biochemical and histopathological changes. Conclusion: The present study suggests that Atriplex halimus extract could be a substantially promising hepatoprotective agent against benzene toxic effects and may be against other hepatotoxic chemical or drugs.

This work aims to study the influence of salt stress of the soil by different concentrations of NaCl (0, 10, 15 g / l) on the water content and some osmolytes of the durum wheat Triticum durum var KEBIR at growth stage and to reduce its effect by spraying the BAP (Phytohormone synthesized: Benzyl amino purine) with concentrations of about 10, 20, 30 mg / l. The results obtained show that the BAP used positively affects on the water content of stressed plants when they are sprayed with the concentrations 20 and 30 mg /l. Similarly, an increase is observed in different contents of soluble sugars, chlorophylls a and b particularly under the effect of the concentration 10 mg / l of BAP. On the other hand, the recorded values showed that the salinity has perfectly affected the concentration or rate of proline on the levels of the plants whether treated by the BAP or not.

The objective of this study is to evaluate the antioxidant and hepatoprotective activity of aerial part and seeds of Coriandrum sativum plant against Lambda cyhalothrin insecticide. Male Wistar Albinos rats were randomly divided into control, LCT, CsA, CsS, CsS+LCT, CsA+LCT groups, after 90 days of treatments Biochemical, some oxidative stress parameters, and histopathology of liver tissue were evaluated. Total polyphenol content in aerial part and the seed extract estimated at 9.29 and 14.64 mg EAG / mg of extract and IC50 for an antioxidant activity equal to 19.38 and 22.62 mg/ml respectively. The obtained results revealed that rats received Lambda cyhalothrin insecticide showed a significant change in enzymes activity (AST, ALT, ALP and c-GT) and Glutathione (GSH) in liver. Meanwhile content of hepatic Malondialdehyde (MDA). Histopathology examination of liver revealed that Coriandrum sativum attenuate the incidence of liver lesions triggered by Lambda cyhalothrin intoxication. T...

Objective: The present study was designed to estimate the preventive and curative potency of aqueous extract of Atriplex halimus L. (Ah) aerial parts against benzene (BZ) intoxication in liver rats. Methods: 30 male albino rats were divided into five groups of 6 rats each: Control, Ah, BZ, AhP+BZ, and BZ+AhC. BZ (100 mg/kg b.w) was added in drinking water for 15 weeks. Aqueous extract of Atriplex halimus was received intragastrically during the last 30 days of BZ exposure for curative treatment (AhC) and all the duration of BZ exposure for preventive treatment (AhP). At the end the experiment, body weight gain and relative liver weight were estimated and liver enzyme markers (AST, ALT, ALP and LDH) were analysed by spectrophotometry. Histopathological studies on hepatic tissue were also performed by the method of Hematoxylin and Eosin staining. Results: Benzene administrated to rats caused an alteration in physiological parameters (body and organs weigh) and hepatic enzymes markers ...

This study was designed to evaluate the effect of natural active principles of a medicinal and aromatic plant: the Sage or Salvia officinalis. To do this, the essential oils were extracted by hydrodistillation, separated and identified by GC/MS. Their antibacterial effect was determined, in vitro on MuellerHinton medium, against some pathogen enterobacteria. Convincing results were obtained. These results showed that the isolated essential oil is constituted of a Chemotype of nineteen (19) terpene compounds with a predominance of α-Thujone (about 37%). The tests of the antibacterial activity showed that the inhibition of the growth depends on the bacterial species and the concentration of the extracted natural product. Generally, the obtained essential oils have a very important antibacterial therapeutic action against the studied multiresistant germs. From all tested strains, three of them were very sensitive face to different concentrations of the obtained plant extract. The recorded inhibition zones often exceed those caused by the used antibiotic (Rifampin 5μg). These results are promising and provide a scientific validation for the massive use of the Sage. Thus the effect of natural active principles isolated from medicinal plants could well rival that of antibiotics.

The aim of the current study was to investigate the preventive and curative effect of Atriplex halimus L. (Ah) extract against the kidney damages induced by Sodium benzoate (SB) in rats. Thirty male albino rats were divided into five groups of 6 rats each: Control, Ah, SB, AhP+SB and SB+AhC. SB (100 mg/kg b.w) was added to drinking water for 15 weeks. Aqueous extract of aerial parts of Atriplex halimus received intragastrically during the last 30 days of SB exposure for curative treatment (AhC) and all the duration of SB exposure for preventive treatment (AhP). Some Biochemical markers, oxidative stress parameters and histopathology of kidney tissue were studied. Administration of Sodium benzoate to rats caused a loss of weight and a significant elevation in creatinine, urea, renal malondialdehyde levels and lactate dehydrogenase activity. These changes were accompanied by decreasing in antioxidant defenses, like reduced glutathione level, catalase and glutathione S transferase acti...