Thymus broussonettii, a moroccan endemic plant, exists in two chemotypes. the aim of our study is to compare the cytotoxic activity of their essential oils and major products as well as their effect on cell cycle and apoptosis induction.... more
Thymus broussonettii, a moroccan endemic plant, exists in two chemotypes. the aim of our study is to compare the cytotoxic activity of their essential oils and major products as well as their effect on cell cycle and apoptosis induction. the chemical composition analysis of essential oils by Gc-sm revealed that the lasts are rich and diverse and the major products of the chemotypes tbA and tbe are carvacrol and thymol, respectively. the in vitro cytotoxic effect study against five tumor cell lines shows that tbA essential oil, rich in carvacrol, has an important cytotoxic effect, higher than that of tbe, rich in thymol. this result is confirmed by comparing cytotoxic effect of carvacrol and thymol. furthermore, tbA eo/carvacrol and tbe eo/thymol induce cell cycle arrest at s and G0/G1 phases, respectively. on the other hand, carvacrol, most cytotoxic in vitro, was studied for its effect on solid tumor in vivo and apoptosis-induction. our results show that carvacrol, administred by g...
The present work aimed to investigate the phenolic composition and the in vitro anticancer activity of ethanol olive cake extracts (OCE) originating in different areas of Tadla-Azilal region in Morocco. The HPLC-MS analysis showed that... more
The present work aimed to investigate the phenolic composition and the in vitro anticancer activity of ethanol olive cake extracts (OCE) originating in different areas of Tadla-Azilal region in Morocco. The HPLC-MS analysis showed that OCE were rich in biophenols mainly composed of secoiridoids and their derivatives, phenolic acids and flavonoids. The bioactivity of these phenolic extracts was evaluated by testing their cytotoxic effect against P815 mastocytoma murine cell line using the MTT assay. The results showed that the OCE exerted an in vitro cytotoxic activity in a dose-dependent manner, and the IC50 values were ranging from 20 to 40 µg/mL. Interestingly, compared to the conventional antitumor drug methotrexate, no cytotoxic effect of OCE was observed on the normal human peripheral blood mononuclear cells (PBMC). Furthermore, the extracts showed an apoptotic effect against P815 tumor cell line depending on the dose and the extract’s phenolic content. Keywords: Phenolic compo...
This investigation aimed to evaluate the in vitro and in vivo antitumor potential of a Moroccan propolis extracts. For in vitro assays, three mammalian tumor cell lines were used: BSR (hamster renal adenocarcinoma), Hep-2 (human laryngeal... more
This investigation aimed to evaluate the in vitro and in vivo antitumor potential of a Moroccan propolis extracts. For in vitro assays, three mammalian tumor cell lines were used: BSR (hamster renal adenocarcinoma), Hep-2 (human laryngeal carcinoma) and P815 (murin mastocytoma). The propolis ethanolic extract as well as the ethyl acetate extract, exert an in vitro cytotoxic activity in dose-dependent manner. The IC50 values were ranging from 15 µg/mL to 38 µg/mL. This activity depends not only on the extract's chemical composition (analysed by HPLC/ESI-MS), but also on the target tumor cells. Interestingly, the cytotoxic effect of these extracts on the normal human peripheral blood mononuclear cells (PBMC) was weak when compared to that induced on tumor cells. On the other hand, oral route treatment of P815 tumor-bearing mice (DBA2/P815) with propolis ethanolic extract (5 mg per mouse every fourth day, fi ve times for group A, and 2.5 mg per mouse every fourth day, fi ve times for group B) signifi cantly reduced the tumor volume (1.2 cm 3 for group A and 2.7 cm 3 for group B at the 22 nd day after tumor graft). These effects are statistically signifi cant as compared to those obtained with the control untreated mice (tumor volume 3.5 cm 3 at day 22).
The aim of this work is to investigate the in vitro cytotoxic and antibacterial effects of the essential oils of Aloysia citriodora Palau, harvested in different regions of Morocco. The chemical profile was established using gas... more
The aim of this work is to investigate the in vitro cytotoxic and antibacterial effects of the essential oils of Aloysia citriodora Palau, harvested in different regions of Morocco. The chemical profile was established using gas chromatography-mass spectrometry analysis. The cytotoxic activity against P815, MCF7, and VERO cell lines as well as the normal human peripheral blood mononuclear cells (PBMCs) was evaluated using the MTT assay. Standard, ATCC, strains of bacteria (Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa) were cultivated in Muller Hinton media. Then, agar disc diffusion, minimum inhibitory concentrations (MICs), and minimal bactericidal concentrations (MBCs) were determined using microdilution method. The essential oils obtained were predominantly composed of í µí»½-spathulenol (15.61%), Ar-curcumene (14.15%), trans-caryophyllene oxide (14.14%), and neral (10.02%). The results of the assays showed that the cytotoxic effect of the essential oil of A. citriodora was high on P815 and moderate on MCF7 and on VERO cell lines. However, no cytotoxic effect was observed on PBMCs. On the other hand, essential oils showed a significant antimicrobial activity against both Gram-negative and Gram-positive bacteria. MICs ranged between 2.84 and 8.37 mg/ml. Essential oil of A. citriodora leaves possesses significant antibacterial effect and cytotoxic activity against tumor cell lines.
This investigation aimed to evaluate the in vitro and in vivo antitumor potential of a Moroccan propolis extracts. For in vitro assays, three mammalian tumor cell lines were used: BSR (hamster renal adenocarcinoma), Hep-2 (human laryngeal... more
This investigation aimed to evaluate the in vitro and in vivo antitumor potential of a Moroccan propolis extracts. For in vitro assays, three mammalian tumor cell lines were used: BSR (hamster renal adenocarcinoma), Hep-2 (human laryngeal carcinoma) and P815 (murin mastocytoma). The propolis ethanolic extract as well as the ethyl acetate extract, exert an in vitro cytotoxic activity in dose-dependent manner. The IC50 values were ranging from 15 µg/mL to 38 µg/mL. This activity depends not only on the extract's chemical composition (analysed by HPLC/ESI-MS), but also on the target tumor cells. Interestingly, the cytotoxic effect of these extracts on the normal human peripheral blood mononuclear cells (PBMC) was weak when compared to that induced on tumor cells. On the other hand, oral route treatment of P815 tumor-bearing mice (DBA2/P815) with propolis ethanolic extract (5 mg per mouse every fourth day, fi ve times for group A, and 2.5 mg per mouse every fourth day, fi ve times for group B) signifi cantly reduced the tumor volume (1.2 cm 3 for group A and 2.7 cm 3 for group B at the 22 nd day after tumor graft). These effects are statistically signifi cant as compared to those obtained with the control untreated mice (tumor volume 3.5 cm 3 at day 22).
The present study aims at defining the differential cytotoxicity effect of artemisinin toward P815 (murin mastocytoma) and BSR (kidney adenocarcinoma of hamster) cell lines. Cytotoxicity was measured by the growth inhibition using MTT... more
The present study aims at defining the differential cytotoxicity effect of artemisinin toward P815 (murin mastocytoma) and BSR (kidney adenocarcinoma of hamster) cell lines. Cytotoxicity was measured by the growth inhibition using MTT assay. These in vitro cytotoxicity studies were complemented by the determination of apoptotic DNA fragmentation and Annexin V-streptavidin-FITC assay. Furthermore, we examined the in vitro synergism between artemisinin and the chemotherapeutic drug, vincristin. The in vivo study was investigated using the DBA2/P815 (H2d) mouse model. While artemisinin acted on both tumor cell lines, P815 was much more sensitive to this drug than BSR cells, as revealed by the respective IC 50 values (12 lM for P815 and 52 lM for BSR cells). On another hand, and interestingly, apoptosis was induced in P815 but not induced in BSR. These data, reveal an interesting differential cytotoxic effect, suggesting the existence of different molecular interactions between artemisinin and the studied cell lines. In vivo, our results clearly showed that the oral administration of artemisinin inhibited solid tumor development. Our study demonstrates that artemisinin caused differential cytotoxic effects depending not only on the concentration and time of exposure but also on the target cells.
Thymus broussonettii, a moroccan endemic plant, exists in two chemotypes. the aim of our study is to compare the cytotoxic activity of their essential oils and major products as well as their effect on cell cycle and apoptosis induction.... more
Thymus broussonettii, a moroccan endemic plant, exists in two chemotypes. the aim of our study is to compare the cytotoxic activity of their essential oils and major products as well as their effect on cell cycle and apoptosis induction. the chemical composition analysis of essential oils by Gc-sm revealed that the lasts are rich and diverse and the major products of the chemotypes tbA and tbe are carvacrol and thymol, respectively. the in vitro cytotoxic effect study against five tumor cell lines shows that tbA essential oil, rich in carvacrol, has an important cytotoxic effect, higher than that of tbe, rich in thymol. this result is confirmed by comparing cytotoxic effect of carvacrol and thymol. furthermore, tbA eo/carvacrol and tbe eo/thymol induce cell cycle arrest at s and G0/G1 phases, respectively. on the other hand, carvacrol, most cytotoxic in vitro, was studied for its effect on solid tumor in vivo and apoptosis-induction. our results show that carvacrol, administred by g...
Macrophytes are one of the main components of the aquatic ecosystem. They are used in several countries as metrics for the ecological assessment of hydrosystems. The objective of our study was to evaluate the suitability of the Macrophyte... more
Macrophytes are one of the main components of the aquatic ecosystem. They are used in several countries as metrics for the ecological assessment of hydrosystems. The objective of our study was to evaluate the suitability of the Macrophyte Biological Index for Rivers (IBMR) to determine a trophic level in the upper Oum Er Rbia basin (Morocco) and to understand physicochemical parameters of water that govern the distribution of macrophyte species. CCA analysis was used to relate the distribution of macrophytes to hydrochemical parameters of water. The CCA analysis shows that the distribution of macrophytes was more correlated with abiotic parameters (EC, WT and DO) than nutrient parameters (PO4-P, NO3-N, NO2-N, NH3-N and CODMn). The recorded values of IBMR in the upper Oum Er Rbia basin indicate that the trophic level of the studied rivers ranged from “moderate” to “very high”. Pearson's correlation coefficient showed that the IBMR is more correlated with the abiotic parameters su...
