Visceral Leishmaniasis is considered by WHO as one of the leading diseases in the world and is present in 88 countries worldwide. It affects poor communities in peripheral areas. It infects the internal organs of the body.,fatal if not... more
Visceral Leishmaniasis is considered by WHO as one of the leading diseases in the world and is present in 88 countries worldwide. It affects poor communities in peripheral areas. It infects the internal organs of the body.,fatal if not treated. It is caused by the parasite L. donovani in the old world and L. infantum in the new world. Correct diagnosis is necessary to treat the disease. Many diagnostic techniques are known, but none is agreed upon worldwide. This study aims to compare two techniques for the diagnosis of VL in Sudan. 37 patients were included in this study. Buffy coat smears were prepared from peripheral blood of these patients and were searched for the presence of the parasite. Serum from these blood samples was used to preform DAT technique. The sensitivity of buffy coat smear was 37.8% and that of DAT was 100% .When MacNemar test was conducted, it was found that DAT is better than buffy coat smear for the diagnosis of VL. We concluded that DAT is a better diagnostic technique and could be adapted for diagnosis of VL in Sudan.
Tel.: +1321287662 Polysaccharides have multiple significant roles and possess extensive bioactivities in field of medicine, healthcare, food and cosmetic industries, because of their therapeutic effects and relatively minimal toxicity.... more
Tel.: +1321287662 Polysaccharides have multiple significant roles and possess extensive bioactivities in field of medicine, healthcare, food and cosmetic industries, because of their therapeutic effects and relatively minimal toxicity. For the first time, we investigated the chemical structure of Ziziphus spina Christ fruits polysaccharides fraction. The plant is classified as one of the most important Sudanese medical plant, still not adequately researched. The Z. spina Christi polysaccharides fraction was extracted by using cold water extraction method, deproteinized via Savage's method, then after participated by using ethanol 80%, the precipitant fractions were dried via lyophilized for 24h. ZSCFPs was investigated by using colorimetric and analytic methods; the colorimetric assays were carried out to determine some functional groups in the polysaccharides fraction such as phenol-sulfuric acid, sulfuric acid-carbazole reaction, ferric chloride reaction. The analytical assays were carried out to confirm the chemical structure of the ZSCFP 80%, which were FTIR spectra, Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), and X-ray diffraction (XRD).
Tel.: +1321287662 Polysaccharides have multiple significant roles and possess extensive bioactivities in field of medicine, healthcare, food and cosmetic industries, because of their therapeutic effects and relatively minimal toxicity.... more
Tel.: +1321287662 Polysaccharides have multiple significant roles and possess extensive bioactivities in field of medicine, healthcare, food and cosmetic industries, because of their therapeutic effects and relatively minimal toxicity. For the first time, we investigated the chemical structure of Ziziphus spina Christ fruits polysaccharides fraction. The plant is classified as one of the most important Sudanese medical plant, still not adequately researched. The Z. spina Christi polysaccharides fraction was extracted by using cold water extraction method, deproteinized via Savage's method, then after participated by using ethanol 80%, the precipitant fractions were dried via lyophilized for 24h. ZSCFPs was investigated by using colorimetric and analytic methods; the colorimetric assays were carried out to determine some functional groups in the polysaccharides fraction such as phenol-sulfuric acid, sulfuric acid-carbazole reaction, ferric chloride reaction. The analytical assays were carried out to confirm the chemical structure of the ZSCFP 80%, which were FTIR spectra, Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), and X-ray diffraction (XRD).
