I have PhD in analytical chemistry and work as professor in college of Education for pure sciences at Mosul Unversity. My work is depend upon development of analytical methods for determination of drugs individual and in a mixtures. I supervised several doctoral and master's degrees.
A simple, sensitive and accurate spectrophotometric method is developed for the quantitative dete... more A simple, sensitive and accurate spectrophotometric method is developed for the quantitative determination of aminophenol isomers, i.e. o-aminophenol, m-aminophenol and p-aminophenol. The method is based on the interaction between these amines and 9-chloroacridine reagent. The spectra of the products show maximum absorption at 436 nm. Beer’s law is obeyed in the concentration range of 0.25-12, 0.2-10 and 0.0-10 µg/ml with molar absorptivity values 7.20х10 3 and 7.67х10 3 L.mol -1 .cm -1 for the above mentioned isomers respectively. The mean percent recoveries are ranged between 97.3% and 101.07 % with relative standard deviation (RSD) ≤ 4.7% for all the isomers. In addition, the stability constant has been determined and the mechanism is proposed for the 9-CA - aminophenol products.
This study involves a development method for spectrophotometric analysis of sulfamethoxazole (SMZ... more This study involves a development method for spectrophotometric analysis of sulfamethoxazole (SMZ) with vanillin reagent in acidic medium. The reaction mechanism was proposed by Schiff's base reaction of the amine group in SMZ with the carbonyl group in vanillin. The spectrum of SMZ complex showed high absorbance at 399.09 nm following the Beer–Lambert law in the concentration levels ranging from 5 to 80 µg.mL-1 with good sensitivity. The stability constant of the product was determined. The values of relative standard deviation (≤ 6.63) and recovery (> 98.73%) indicated a reasonably precise and accurate method with a good limit of detection and quantification. The present method was compared with other spectrophotometric methods in the literature. The procedure of the method showed to be simple since no need for temperature control and solvent extraction. The suggested method was used to determine of SMZ in commercial pharmaceutical drops and compared with the certified method.
A spectrophotometric method is developed for the determination of some drugs containing amino gro... more A spectrophotometric method is developed for the determination of some drugs containing amino groups (sulfacetamide sodium, lidocaine and terbutaline sulfate) based on their reaction with p-chloranilic acid reagent in an organic medium forming colored charge transfer complexes. The complexes have maximum absorptions at 530 and 527 nm for sulfacetamide sodium and lidocaine respectively, but terbutaline sulfate gave two maximum absorptions at 529 and 319 nm. Beers law is obeyed over the concentration range of 10-60 μg.ml-1 for sulfacetamide sodium and lidocaine and 5-70 μg.ml-1 for terbutaline sulfate. The molar absorptivity values are 0.940×103, 0.913×103 L.mol-1.cm-1 for sulfacetamide sodium and lidocaine respectively while terbutaline sulfate gave 0.987×103 L.mol-1.cm-1 at 529 nm and 7.407×103 L.mol-1.cm-1 at 319 nm with accuracy range between 100.20% and 101.42% and RSD better than 3.15% for all drugs. The method is applied successfully for determination of these drugs in pharmace...
A simple, sensitive and selective spectrophotometric method is described for the determination of... more A simple, sensitive and selective spectrophotometric method is described for the determination of dapson and nitrazepam in bulk drug and in dosage forms using vanillin as the chromogenic agent. The method is based on the condensation reaction between dapson (DN) and reduced nitrazepam (RNZ) with vanillin to produce an intense yellow colored product. The resulting Schiff’s bases show an absorption maximum at 405 nm for both drugs. Beer’s law was obeyed in the concentration range 1-30 and 1-20 µg/ml with a molar absorptivity of 8.69×103 and 1.332×104 L mol-1 cm-1 for DN and RNZ respectively. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.087 and 0.299 µg/ml for DN, respectively and 0.069 and 0.231 µg/ml for RNZ, respectively. The method shows good accuracy and precision, and was applied successfully to the determination of DN and RNZ in tablets. No interference was observed from common additives found in pharmaceutical preparations. The results obtai...
Two simple, sensitive and accurate spectrophotometric methods for the determination of mesalamine... more Two simple, sensitive and accurate spectrophotometric methods for the determination of mesalamine are described. The methods are based on charge-transfer complex formation reactions of the drug with two π-electron acceptors, p-chloranil and p-fluoranil, in aqueous medium. The coloured complexes having maximum absorbance at 346 nm and 347 nm by using p-chloranil and p-fluoranil reagents respectively. Under the optimized experimental conditions, the calibration curves showed a linear relationship over the concentration ranges of 1–35 and 1-30 μg ml−1 with molar absorptivity values 4.60×103 and 5.67×103 L mol-1 cm-1. using above reagents respectively. The limits of detection and quantitation were found 0.376 and 1.25 μg ml−1 using p-chloranil, respectively, and 0.333 and 1.111 μg ml−1 using p-fluoranil, respectively. The complexes were found to be formed in ratio of 1:1 species in both methods, with stability constants of 6.9104 and 5.1105 L mol-1 for mesalamine complexes with p-chlo...
Official methods used for the determination of these drugs in pharmaceutical preparations are usu... more Official methods used for the determination of these drugs in pharmaceutical preparations are usually based on the extraction of a freebase and subsequent determi-nation by UV-spectrophotometry [15, 16]. Many organic compounds, drug excipients, and diluents (as well as various ...
A simple, accurate and sensitive spectrophotometric method is described for the determination of ... more A simple, accurate and sensitive spectrophotometric method is described for the determination of ampicillin and amoxicillin in pure forms and in pharmaceutical formulations. The method is based on the reaction of these drugs in aqueous solution with tetracyanoethylene reagent in the presence of sodium hydroxide to produce a yellow coloured species measurable at λmax 322 and 325nm for ampicillin and amoxicillin respectively. The method has been used for the determination of 1.0-40 µg/ml for both drugs. The complexes have apparent molar absorptivities of 7.545×103 and 9.915×103 L.mol-1.cm-1 and Sandell sensitivities of 0.0532 and 0.042 µg.cm-2 for above drugs respectively. Also, it was found that these products were formed in ratio of 1:1 species with stability constants of 7.96104 and 3.91104L/mol for ampicillin and amoxicillin respectively. The method was applied successfully to the assay of ampicillin and amoxicillin in their pharmaceutical formulations and was agreed well with its certified value and with the standard addition procedure.
A simple, sensitive and accurate spectrophotometric method is developed for the quantitative dete... more A simple, sensitive and accurate spectrophotometric method is developed for the quantitative determination of aminophenol isomers, i.e. o-aminophenol, m-aminophenol and p-aminophenol. The method is based on the interaction between these amines and 9-chloroacridine reagent. The spectra of the products show maximum absorption at 436 nm. Beer’s law is obeyed in the concentration range of 0.25-12, 0.2-10 and 0.0-10 µg/ml with molar absorptivity values 7.20х10 3 and 7.67х10 3 L.mol -1 .cm -1 for the above mentioned isomers respectively. The mean percent recoveries are ranged between 97.3% and 101.07 % with relative standard deviation (RSD) ≤ 4.7% for all the isomers. In addition, the stability constant has been determined and the mechanism is proposed for the 9-CA - aminophenol products.
This study involves a development method for spectrophotometric analysis of sulfamethoxazole (SMZ... more This study involves a development method for spectrophotometric analysis of sulfamethoxazole (SMZ) with vanillin reagent in acidic medium. The reaction mechanism was proposed by Schiff's base reaction of the amine group in SMZ with the carbonyl group in vanillin. The spectrum of SMZ complex showed high absorbance at 399.09 nm following the Beer–Lambert law in the concentration levels ranging from 5 to 80 µg.mL-1 with good sensitivity. The stability constant of the product was determined. The values of relative standard deviation (≤ 6.63) and recovery (> 98.73%) indicated a reasonably precise and accurate method with a good limit of detection and quantification. The present method was compared with other spectrophotometric methods in the literature. The procedure of the method showed to be simple since no need for temperature control and solvent extraction. The suggested method was used to determine of SMZ in commercial pharmaceutical drops and compared with the certified method.
A spectrophotometric method is developed for the determination of some drugs containing amino gro... more A spectrophotometric method is developed for the determination of some drugs containing amino groups (sulfacetamide sodium, lidocaine and terbutaline sulfate) based on their reaction with p-chloranilic acid reagent in an organic medium forming colored charge transfer complexes. The complexes have maximum absorptions at 530 and 527 nm for sulfacetamide sodium and lidocaine respectively, but terbutaline sulfate gave two maximum absorptions at 529 and 319 nm. Beers law is obeyed over the concentration range of 10-60 μg.ml-1 for sulfacetamide sodium and lidocaine and 5-70 μg.ml-1 for terbutaline sulfate. The molar absorptivity values are 0.940×103, 0.913×103 L.mol-1.cm-1 for sulfacetamide sodium and lidocaine respectively while terbutaline sulfate gave 0.987×103 L.mol-1.cm-1 at 529 nm and 7.407×103 L.mol-1.cm-1 at 319 nm with accuracy range between 100.20% and 101.42% and RSD better than 3.15% for all drugs. The method is applied successfully for determination of these drugs in pharmace...
A simple, sensitive and selective spectrophotometric method is described for the determination of... more A simple, sensitive and selective spectrophotometric method is described for the determination of dapson and nitrazepam in bulk drug and in dosage forms using vanillin as the chromogenic agent. The method is based on the condensation reaction between dapson (DN) and reduced nitrazepam (RNZ) with vanillin to produce an intense yellow colored product. The resulting Schiff’s bases show an absorption maximum at 405 nm for both drugs. Beer’s law was obeyed in the concentration range 1-30 and 1-20 µg/ml with a molar absorptivity of 8.69×103 and 1.332×104 L mol-1 cm-1 for DN and RNZ respectively. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.087 and 0.299 µg/ml for DN, respectively and 0.069 and 0.231 µg/ml for RNZ, respectively. The method shows good accuracy and precision, and was applied successfully to the determination of DN and RNZ in tablets. No interference was observed from common additives found in pharmaceutical preparations. The results obtai...
Two simple, sensitive and accurate spectrophotometric methods for the determination of mesalamine... more Two simple, sensitive and accurate spectrophotometric methods for the determination of mesalamine are described. The methods are based on charge-transfer complex formation reactions of the drug with two π-electron acceptors, p-chloranil and p-fluoranil, in aqueous medium. The coloured complexes having maximum absorbance at 346 nm and 347 nm by using p-chloranil and p-fluoranil reagents respectively. Under the optimized experimental conditions, the calibration curves showed a linear relationship over the concentration ranges of 1–35 and 1-30 μg ml−1 with molar absorptivity values 4.60×103 and 5.67×103 L mol-1 cm-1. using above reagents respectively. The limits of detection and quantitation were found 0.376 and 1.25 μg ml−1 using p-chloranil, respectively, and 0.333 and 1.111 μg ml−1 using p-fluoranil, respectively. The complexes were found to be formed in ratio of 1:1 species in both methods, with stability constants of 6.9104 and 5.1105 L mol-1 for mesalamine complexes with p-chlo...
Official methods used for the determination of these drugs in pharmaceutical preparations are usu... more Official methods used for the determination of these drugs in pharmaceutical preparations are usually based on the extraction of a freebase and subsequent determi-nation by UV-spectrophotometry [15, 16]. Many organic compounds, drug excipients, and diluents (as well as various ...
A simple, accurate and sensitive spectrophotometric method is described for the determination of ... more A simple, accurate and sensitive spectrophotometric method is described for the determination of ampicillin and amoxicillin in pure forms and in pharmaceutical formulations. The method is based on the reaction of these drugs in aqueous solution with tetracyanoethylene reagent in the presence of sodium hydroxide to produce a yellow coloured species measurable at λmax 322 and 325nm for ampicillin and amoxicillin respectively. The method has been used for the determination of 1.0-40 µg/ml for both drugs. The complexes have apparent molar absorptivities of 7.545×103 and 9.915×103 L.mol-1.cm-1 and Sandell sensitivities of 0.0532 and 0.042 µg.cm-2 for above drugs respectively. Also, it was found that these products were formed in ratio of 1:1 species with stability constants of 7.96104 and 3.91104L/mol for ampicillin and amoxicillin respectively. The method was applied successfully to the assay of ampicillin and amoxicillin in their pharmaceutical formulations and was agreed well with its certified value and with the standard addition procedure.
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Papers by Theia'a N Al-Sabha