The study aims to perform, for the first time, the molecular identification of anisakid larvae in commercial fish from the Southeastern Pacific Ocean off the Peru coast, and to provide data on their infection level by fishing ground, fish... more
The study aims to perform, for the first time, the molecular identification of anisakid larvae in commercial fish from the Southeastern Pacific Ocean off the Peru coast, and to provide data on their infection level by fishing ground, fish host, and site of infection. Fish specimens (N = 348) from the northern and the central coast of Peru were examined for parasites. The fish fillets were examined by the UV-press method. Anisakis spp. larvae (N = 305) were identified by mtDNA cox2 sequences analysis and by the ARMS-PCR of the locus nas10 nDNA. Two hundred and eighty-eight Anisakis Type I larvae corresponded to Anisakis pegreffii, whereas 17 Anisakis Type II larvae clustered in a phylogenetic lineage distinct from Anisakis physeteris deposited in GenBank, and corresponding to a phylogenetic lineage indicated as Anisakis sp. 2, previously detected in fish from both Pacific and Atlantic waters. Anisakis pegreffii was found to infect both the flesh and viscera, while Anisakis sp. 2 occu...
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El presente estudio tuvo como objetivo caracterizar la frecuencia de neoplasias caninas diagnosticadas por histopatología durante el periodo del 2003 al 2015 en el Laboratorio de Histología y Patología Veterinaria de la Universidad... more
El presente estudio tuvo como objetivo caracterizar la frecuencia de neoplasias caninas diagnosticadas por histopatología durante el periodo del 2003 al 2015 en el Laboratorio de Histología y Patología Veterinaria de la Universidad Peruana Cayetano Heredia. Se analizaron un total de 2620 casos, de los cuales solo el 46.3% fueron incluidos en el estudio. El diagnóstico morfológico fue reclasificado de acuerdo con la "Clasificación Histológica Internacional de Tumores de Animales Domésticos" (OMS-AFIP), excluyendo los tumores mamarios que fueron clasificados de acuerdo con Goldschmidt et al. (2011). El sexo, la edad, la raza, la ubicación anatómica de la muestra, las características morfológicas o clínicas y el grado de malignidad se registraron y analizaron mediante análisis estadístico. El Carcinoma de células escamosas fue la neoplasia más frecuente (7.7%), siendo las hembras y los pacientes geriátricos los más afectados (60.7 y 54.4 % respectivamente). La raza cruzada pr...
Blastocystis is a common intestinal protist distributed worldwide, infecting humans and a wide range of domestic and wild animals. It exhibits an extensive genetic diversity and, so far, 25 distinct small subunit ribosomal RNA (SSU rRNA)... more
Blastocystis is a common intestinal protist distributed worldwide, infecting humans and a wide range of domestic and wild animals. It exhibits an extensive genetic diversity and, so far, 25 distinct small subunit ribosomal RNA (SSU rRNA) lineages termed subtypes (STs)) have been characterized; among them, 12 have thus far been reported in humans. The aims of the present study were to detect and genetically characterize Blastocystis sp. in synantropic animals to improve our current knowledge on the distribution and zoonotic transmission of Blastocystis STs in Italy. Samples were collected from N = 193 farmed animals and submitted to DNA extraction and PCR amplification of the SSU rRNA. Blastocystis was detected in 60 samples (31.08%) and successfully subtyped. Phylogenetic analysis evidenced that the isolates from fallow deer, goats, and pigs (N = 9) clustered within the ST5; those from pheasants (N = 2) in the ST6; those from chickens (N = 8) in the ST7; those from sheep (N = 6) in ...