Proceedings of the National Academy of Sciences, 1975
Activated thiol-Sepharose [agarose-(glutathione-2-pyridyl disulfide) conjugate] has been used to ... more Activated thiol-Sepharose [agarose-(glutathione-2-pyridyl disulfide) conjugate] has been used to immobilize proteins with a single or a few thiol groups via disulfide bridges. The immobilized proteins were subsequently proteolytically degraded. After washing, the thiol-containing peptides were eluted with a reducing agent. A single preparative paper electrophoresis, occasionally after a modification such as oxidation, was sufficient to obtain pure peptides in good yields. The method was applied to the major parvalbumin from hake muscle (a protein with 108 amino acid residues and one cysteine residue), to mercaptalbumin from bovine serum (565 residues and one cysteine), and to human serum ferroxidase [EC 1.16.3.1; iron (II):oxygen oxidoreductase] (ceruloplasmin) (1065 residues and three cysteines). The use of the technique, e.g., as a simple means of obtaining homologous peptides in related proteins, is discussed.
Ciencia para la paz y el desarrollo: el caso del …
117 El Juramento de Buenos Aires: Una ética de derechos, deberes y responsabilidades humanas para... more 117 El Juramento de Buenos Aires: Una ética de derechos, deberes y responsabilidades humanas para la ciencia MC Patricia Morales Dedicado a Carlos A. Mallmann y Gustavo Malek Resumen: El artículo se ocupa de considerar el logro del Juramento de Bue-nos Aires. A ...
Proceedings of the National Academy of Sciences, 1984
We have determined the amino acid sequence of the amino-terminal 67,000-dalton (67-kDa) fragment ... more We have determined the amino acid sequence of the amino-terminal 67,000-dalton (67-kDa) fragment of human ceruloplasmin and have established overlapping sequences between the 67-kDa and 50-kDa fragments and between the 50-kDa and 19-kDa fragments. The 67-kDa fragment contains 480 amino acid residues and three glucosamine oligosaccharides. These results together with our previous sequence data for the 50-kDa and 19-kDa fragments complete the amino acid sequence of human ceruloplasmin. The polypeptide chain has a total of 1,046 amino acid residues (Mr 120,085) and has attachment sites for four glucosamine oligosaccharides; together these account for the total molecular mass of human ceruloplasmin (132 kDa). The sequence analysis of the peptides overlapping the fragments showed that one additional amino acid, arginine, is present between the 67-kDa and 50-kDa fragments, and another, lysine, is between the 50-kDa and 19-kDa fragments. Only two apparent sites of amino acid interchange ha...
The venom of the Australian tiger snake Notechis scutatus scutatus has been fractionated by ion-e... more The venom of the Australian tiger snake Notechis scutatus scutatus has been fractionated by ion-exchange chromatography on Bio-Rex 70 in an ammonium acetate gradient. The main neurotoxic component, the notexin, which constituted 6 per cent of the crude venom, was thereby ...
In a double-blind randomized trial, 1,395 patients with suspected acute myocardial infarction (MI... more In a double-blind randomized trial, 1,395 patients with suspected acute myocardial infarction (MI) were investigated to evaluate the possibility of limiting indirect signs of the size and severity of acute MI with the beta 1 -selective adrenoceptor antagonist metoprolol. Metoprolol (15 ...
Proceedings of the National Academy of Sciences, 1975
Activated thiol-Sepharose [agarose-(glutathione-2-pyridyl disulfide) conjugate] has been used to ... more Activated thiol-Sepharose [agarose-(glutathione-2-pyridyl disulfide) conjugate] has been used to immobilize proteins with a single or a few thiol groups via disulfide bridges. The immobilized proteins were subsequently proteolytically degraded. After washing, the thiol-containing peptides were eluted with a reducing agent. A single preparative paper electrophoresis, occasionally after a modification such as oxidation, was sufficient to obtain pure peptides in good yields. The method was applied to the major parvalbumin from hake muscle (a protein with 108 amino acid residues and one cysteine residue), to mercaptalbumin from bovine serum (565 residues and one cysteine), and to human serum ferroxidase [EC 1.16.3.1; iron (II):oxygen oxidoreductase] (ceruloplasmin) (1065 residues and three cysteines). The use of the technique, e.g., as a simple means of obtaining homologous peptides in related proteins, is discussed.
Ciencia para la paz y el desarrollo: el caso del …
117 El Juramento de Buenos Aires: Una ética de derechos, deberes y responsabilidades humanas para... more 117 El Juramento de Buenos Aires: Una ética de derechos, deberes y responsabilidades humanas para la ciencia MC Patricia Morales Dedicado a Carlos A. Mallmann y Gustavo Malek Resumen: El artículo se ocupa de considerar el logro del Juramento de Bue-nos Aires. A ...
Proceedings of the National Academy of Sciences, 1984
We have determined the amino acid sequence of the amino-terminal 67,000-dalton (67-kDa) fragment ... more We have determined the amino acid sequence of the amino-terminal 67,000-dalton (67-kDa) fragment of human ceruloplasmin and have established overlapping sequences between the 67-kDa and 50-kDa fragments and between the 50-kDa and 19-kDa fragments. The 67-kDa fragment contains 480 amino acid residues and three glucosamine oligosaccharides. These results together with our previous sequence data for the 50-kDa and 19-kDa fragments complete the amino acid sequence of human ceruloplasmin. The polypeptide chain has a total of 1,046 amino acid residues (Mr 120,085) and has attachment sites for four glucosamine oligosaccharides; together these account for the total molecular mass of human ceruloplasmin (132 kDa). The sequence analysis of the peptides overlapping the fragments showed that one additional amino acid, arginine, is present between the 67-kDa and 50-kDa fragments, and another, lysine, is between the 50-kDa and 19-kDa fragments. Only two apparent sites of amino acid interchange ha...
The venom of the Australian tiger snake Notechis scutatus scutatus has been fractionated by ion-e... more The venom of the Australian tiger snake Notechis scutatus scutatus has been fractionated by ion-exchange chromatography on Bio-Rex 70 in an ammonium acetate gradient. The main neurotoxic component, the notexin, which constituted 6 per cent of the crude venom, was thereby ...
In a double-blind randomized trial, 1,395 patients with suspected acute myocardial infarction (MI... more In a double-blind randomized trial, 1,395 patients with suspected acute myocardial infarction (MI) were investigated to evaluate the possibility of limiting indirect signs of the size and severity of acute MI with the beta 1 -selective adrenoceptor antagonist metoprolol. Metoprolol (15 ...
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