The expression of the cell wall protein extensin, a hydroxyproline-rich glycoprotein, is induced ... more The expression of the cell wall protein extensin, a hydroxyproline-rich glycoprotein, is induced by several different stimuli, including wounding. The process of protoplast preparation mimics the wounding effect and results in the induction of extensin. Using transient expression in protoplasts we analyzed several deletions of the extensin promoter. We identified an important transcriptional regulatory element located between the two TATA
The eukaryotic translation elongation factor eIF5A is the only protein known to contain the unusu... more The eukaryotic translation elongation factor eIF5A is the only protein known to contain the unusual amino acid hypusine which is essential for its biological activity. This post-translational modification is achieved by the sequential action of the enzymes deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH). The crucial molecular function of eIF5A during translation has been recently elucidated in yeast and it is expected to be fully conserved in every eukaryotic cell, however the functional description of this pathway in plants is still sparse. The genetic approaches with transgenic plants for either eIF5A overexpression or antisense have revealed some activities related to the control of cell death processes but the molecular details remain to be characterized. One important aspect of fully understanding this pathway is the biochemical description of the hypusine modification system. Here we have used recombinant eIF5A proteins either modified by hypusination or non-...
... Diego Orzáez,; Antonio Granell. Article first published online: 5 MAR 2002. ... Departamento ... more ... Diego Orzáez,; Antonio Granell. Article first published online: 5 MAR 2002. ... Departamento de Biología del Desarrollo, Instituto de Biologia Molecular y Celular de Plantas (UPV-CSIC), Caminode Vera 14, 46022 Valencia, Spain. *Correspondence: Antonio Granell,. ...
The expression of the cell wall protein extensin, a hydroxyproline-rich glycoprotein, is induced ... more The expression of the cell wall protein extensin, a hydroxyproline-rich glycoprotein, is induced by several different stimuli, including wounding. The process of protoplast preparation mimics the wounding effect and results in the induction of extensin. Using transient expression in protoplasts we analyzed several deletions of the extensin promoter. We identified an important transcriptional regulatory element located between the two TATA
The eukaryotic translation elongation factor eIF5A is the only protein known to contain the unusu... more The eukaryotic translation elongation factor eIF5A is the only protein known to contain the unusual amino acid hypusine which is essential for its biological activity. This post-translational modification is achieved by the sequential action of the enzymes deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH). The crucial molecular function of eIF5A during translation has been recently elucidated in yeast and it is expected to be fully conserved in every eukaryotic cell, however the functional description of this pathway in plants is still sparse. The genetic approaches with transgenic plants for either eIF5A overexpression or antisense have revealed some activities related to the control of cell death processes but the molecular details remain to be characterized. One important aspect of fully understanding this pathway is the biochemical description of the hypusine modification system. Here we have used recombinant eIF5A proteins either modified by hypusination or non-...
... Diego Orzáez,; Antonio Granell. Article first published online: 5 MAR 2002. ... Departamento ... more ... Diego Orzáez,; Antonio Granell. Article first published online: 5 MAR 2002. ... Departamento de Biología del Desarrollo, Instituto de Biologia Molecular y Celular de Plantas (UPV-CSIC), Caminode Vera 14, 46022 Valencia, Spain. *Correspondence: Antonio Granell,. ...
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