Photobiomodulation, Photomedicine, and Laser Surgery
Background. The lipid metabolism is essential for maintaining the body's energy responses... more Background. The lipid metabolism is essential for maintaining the body's energy responses. Laser photobiomodulation triggers many important cellular effects, but these effects on lipid metabolism are not well described. In this study, we analyzed the laser photobiomodulation in the Lipase Sensitive Hormone activity, a key enzyme in the triglycerides hydrolysis in adipose tissue. 3T3-L1 Methods. Cells were submitted to the differentiation protocol in adipose cells, irradiated with 1J, 2J and 3J with laser (904 nm - 60 mw - laser diode) and incubated for 4 hours after irradiation. Results. The response of laser photobiomodulation was able to trigger an inhibition of Hormone-Sensitive Lipase activity (control = 0,057 ± 0,0008; 1J = 0,050 ± 0,0003; 2J = 0,0477 ± 0,002; 3J = 0,051 ± 0,002. p = 0,0003 against the control), but no modulations was observed in triglycerides levels into the medium (control =26,5856 ± 0,52; 1J = 26,5856 ± 0,52; 2J = 27,2372 ± 1,41; 3J = 25,9991 ± 0,1303, p = 0,18). Conclusion. This is the first describe of Hormone-Sensitive Lipase activity modulation with laser radiation, suggesting that photobiomodulation can influence adipose tissue metabolism and open a new field of study.
Angiotensin-I-converting enzyme (ACE) is involved in the synthesis and degradation of important b... more Angiotensin-I-converting enzyme (ACE) is involved in the synthesis and degradation of important bioactive peptides. The ACE gene has a 287-bp insertion/deletion polymorphism that controls ACE expression through a mechanism that remains elusive. In this study, we found that the 287-bp polymorphic element of the ACE gene, a member of the AluYa5 sub-family of Alu elements, codes for an RNA molecule that controls the levels of ACE mRNA. Transient transfection of a plasmid containing a CMV promoter upstream of the ACE polymorphic element resulted in significant expression of an AluYa5 RNA and reduced ACE mRNA expression as well as ACE enzymatic activity in AD 293 cells. The AluYa5 element also independently reduced the expression of other genes, regardless of whether these genes harbored Alu elements within their genomic context. Interestingly, the CMV promoter was not required for the expression of the AluYa5 element in AD 293 cells. The 287-bp sequence was sufficient to produce AluYa5 ...
Acute kidney injury (AKI) is mostly reversible and hepatocyte growth factor (HGF) has a relevant ... more Acute kidney injury (AKI) is mostly reversible and hepatocyte growth factor (HGF) has a relevant role in the tissue repair. MicroRNA (miR)-26a is an endogenous modulator of HGF. The role of miR-26a in the kidney repair process was evaluated in Wistar rats submitted to an AKI model of rhabdomyolysis induced by glycerol (6 mL/kg). Animals were evaluated 3, 12, 48, 96 and 120 hr after glycerol injection. Serum creatinine (SCr) and gene expression of HGF, c-met, STAT3 and miR-26a were estimated. Also, tubular NK52E cells were transfected with anti-miR26a and stimulated with Fe3+ for 24 hr to mimic the effects of myoglobin in vitro. SCr was highest after 48 hr. After 96 hr, SCr started to decrease, characterizing the recovery phase, with normalization after 120 hr. HGF expression increased during the onset phase (3 hr), with a low relationship with miR-26a. In contrast, in the recovery phase, the increase in miR-26a was coincident with HGF mRNA suppression, suggesting that in the recover...
Photobiomodulation, Photomedicine, and Laser Surgery
Background. The lipid metabolism is essential for maintaining the body's energy responses... more Background. The lipid metabolism is essential for maintaining the body's energy responses. Laser photobiomodulation triggers many important cellular effects, but these effects on lipid metabolism are not well described. In this study, we analyzed the laser photobiomodulation in the Lipase Sensitive Hormone activity, a key enzyme in the triglycerides hydrolysis in adipose tissue. 3T3-L1 Methods. Cells were submitted to the differentiation protocol in adipose cells, irradiated with 1J, 2J and 3J with laser (904 nm - 60 mw - laser diode) and incubated for 4 hours after irradiation. Results. The response of laser photobiomodulation was able to trigger an inhibition of Hormone-Sensitive Lipase activity (control = 0,057 ± 0,0008; 1J = 0,050 ± 0,0003; 2J = 0,0477 ± 0,002; 3J = 0,051 ± 0,002. p = 0,0003 against the control), but no modulations was observed in triglycerides levels into the medium (control =26,5856 ± 0,52; 1J = 26,5856 ± 0,52; 2J = 27,2372 ± 1,41; 3J = 25,9991 ± 0,1303, p = 0,18). Conclusion. This is the first describe of Hormone-Sensitive Lipase activity modulation with laser radiation, suggesting that photobiomodulation can influence adipose tissue metabolism and open a new field of study.
Angiotensin-I-converting enzyme (ACE) is involved in the synthesis and degradation of important b... more Angiotensin-I-converting enzyme (ACE) is involved in the synthesis and degradation of important bioactive peptides. The ACE gene has a 287-bp insertion/deletion polymorphism that controls ACE expression through a mechanism that remains elusive. In this study, we found that the 287-bp polymorphic element of the ACE gene, a member of the AluYa5 sub-family of Alu elements, codes for an RNA molecule that controls the levels of ACE mRNA. Transient transfection of a plasmid containing a CMV promoter upstream of the ACE polymorphic element resulted in significant expression of an AluYa5 RNA and reduced ACE mRNA expression as well as ACE enzymatic activity in AD 293 cells. The AluYa5 element also independently reduced the expression of other genes, regardless of whether these genes harbored Alu elements within their genomic context. Interestingly, the CMV promoter was not required for the expression of the AluYa5 element in AD 293 cells. The 287-bp sequence was sufficient to produce AluYa5 ...
Acute kidney injury (AKI) is mostly reversible and hepatocyte growth factor (HGF) has a relevant ... more Acute kidney injury (AKI) is mostly reversible and hepatocyte growth factor (HGF) has a relevant role in the tissue repair. MicroRNA (miR)-26a is an endogenous modulator of HGF. The role of miR-26a in the kidney repair process was evaluated in Wistar rats submitted to an AKI model of rhabdomyolysis induced by glycerol (6 mL/kg). Animals were evaluated 3, 12, 48, 96 and 120 hr after glycerol injection. Serum creatinine (SCr) and gene expression of HGF, c-met, STAT3 and miR-26a were estimated. Also, tubular NK52E cells were transfected with anti-miR26a and stimulated with Fe3+ for 24 hr to mimic the effects of myoglobin in vitro. SCr was highest after 48 hr. After 96 hr, SCr started to decrease, characterizing the recovery phase, with normalization after 120 hr. HGF expression increased during the onset phase (3 hr), with a low relationship with miR-26a. In contrast, in the recovery phase, the increase in miR-26a was coincident with HGF mRNA suppression, suggesting that in the recover...
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Papers by Pedro Gattai