A b s t r a c t . The karyotype of bream Abramis brama was analysed by means of C-banding, replic... more A b s t r a c t . The karyotype of bream Abramis brama was analysed by means of C-banding, replication banding, DAPI fluorescent staining and in situ hybridisation with 18S rDNA and telomeric probes. The use of the in vivo 5-bromodeoxyuridine incorporation technique enabled the induction of replication bands of the RBA type in the karyotype. C-bands corresponded to late replicated chromosome regions. 18S rDNA clusters were found on one chromosome pair. Telomeric sequences were observed only on ends of chromatids. The karyotype morphology and NOR phenotype of A. brama are very similar to those found in other species of the leuciscine cyprinids karyotyped so far.
We analyzed the DNA content of hepatocyte and erythrocyte nuclei of the spined loach Cobitis taen... more We analyzed the DNA content of hepatocyte and erythrocyte nuclei of the spined loach Cobitis taenia (diploid) and its allopolyploid forms. Twenty triploid females and one tetraploid were used. At least 20,000 hepatocyte and erythrocyte nuclei were acquired and analyzed by flow cytometry. C. taenia erythrocyte nuclei contain 3.15 ± 0.21 pg of DNA and the hepatocyte nuclei 4.45 ± 0.46 pg of DNA. Triploid Cobitis have 5.08 ± 0.41 pg of DNA in erythrocyte nuclei and 6.11 ± 0.40 pg of DNA in hepatocyte nuclei, whereas the tetraploid erythrocyte and hepatocyte nuclei contained 6.60 and 7.40 pg of DNA, respectively. In general, the DNA contents correlate positively with the ploidy level of the fish investigated. The DNA content variation in the hepatocyte and erythrocyte nuclei may be due to differences in extent of chromatin condensation, which is more pronounced in the erythrocyte than hepatocyte nuclei, or to the several orders of ploidy that occur in the parenchymal liver cells.
The history of the decline in barbel abundance and distribution in the Warta River system is desc... more The history of the decline in barbel abundance and distribution in the Warta River system is described. This fish, formerly widely distributed in the Warta and its larg-er tributaries, is nowadays confined mainly to the upper Warta, where it is still rela-tively abundant, although not ...
A bstract . The variability of morphological characters was studied in a population of spined loa... more A bstract . The variability of morphological characters was studied in a population of spined loach from Klawój Lake karyologically identified as a pure diploid C. taenia (2n = 48). 24 metric features were used to calculate correlation coefficients and linear regressions to determine the ...
A b s t r a c t . The karyotype of bream Abramis brama was analysed by means of C-banding, replic... more A b s t r a c t . The karyotype of bream Abramis brama was analysed by means of C-banding, replication banding, DAPI fluorescent staining and in situ hybridisation with 18S rDNA and telomeric probes. The use of the in vivo 5-bromodeoxyuridine incorporation technique enabled the induction of replication bands of the RBA type in the karyotype. C-bands corresponded to late replicated chromosome regions. 18S rDNA clusters were found on one chromosome pair. Telomeric sequences were observed only on ends of chromatids. The karyotype morphology and NOR phenotype of A. brama are very similar to those found in other species of the leuciscine cyprinids karyotyped so far.
We analyzed the DNA content of hepatocyte and erythrocyte nuclei of the spined loach Cobitis taen... more We analyzed the DNA content of hepatocyte and erythrocyte nuclei of the spined loach Cobitis taenia (diploid) and its allopolyploid forms. Twenty triploid females and one tetraploid were used. At least 20,000 hepatocyte and erythrocyte nuclei were acquired and analyzed by flow cytometry. C. taenia erythrocyte nuclei contain 3.15 ± 0.21 pg of DNA and the hepatocyte nuclei 4.45 ± 0.46 pg of DNA. Triploid Cobitis have 5.08 ± 0.41 pg of DNA in erythrocyte nuclei and 6.11 ± 0.40 pg of DNA in hepatocyte nuclei, whereas the tetraploid erythrocyte and hepatocyte nuclei contained 6.60 and 7.40 pg of DNA, respectively. In general, the DNA contents correlate positively with the ploidy level of the fish investigated. The DNA content variation in the hepatocyte and erythrocyte nuclei may be due to differences in extent of chromatin condensation, which is more pronounced in the erythrocyte than hepatocyte nuclei, or to the several orders of ploidy that occur in the parenchymal liver cells.
The history of the decline in barbel abundance and distribution in the Warta River system is desc... more The history of the decline in barbel abundance and distribution in the Warta River system is described. This fish, formerly widely distributed in the Warta and its larg-er tributaries, is nowadays confined mainly to the upper Warta, where it is still rela-tively abundant, although not ...
A bstract . The variability of morphological characters was studied in a population of spined loa... more A bstract . The variability of morphological characters was studied in a population of spined loach from Klawój Lake karyologically identified as a pure diploid C. taenia (2n = 48). 24 metric features were used to calculate correlation coefficients and linear regressions to determine the ...
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