Although extensive literature exists on antioxidant properties of medicinal plants, very few stud... more Although extensive literature exists on antioxidant properties of medicinal plants, very few studies have focused on their polyphenolic composition. Here, preliminary phytochemical screening and total flavonoid content in extracts of seeds of Myristica fragrans and leaves of Cordyline terminalis have been investigated. Screening of potential flavonoid-subclasses was done by LC-ESI-MS, whose data were interpreted using database of Lipid Metabolites and Pathways Strategy Consortium. ‘Flavones and Flavonols’ and ‘Anthocyanidins’ appear to be more abundant in C. terminalis than in M. fragrans. Higher content of isoflavonoids, chalcones and dihydrochalcones and some isoprenoids were observed in M. fragrans than in C. terminalis. The strategy and results of this study will help to choose appropriate standards prior to their identification and confirmation in natural extracts. These results may also be useful to assess antioxidant activity of only the subclass alone and accordingly appropr...
Aloe vera (L.) Burm. F (Asphodelaceae) is a traditional medicinal plant having multiple biologica... more Aloe vera (L.) Burm. F (Asphodelaceae) is a traditional medicinal plant having multiple biological activities such as wound healing, anticancer and antidiabetic activity, etc. These properties have been attributed to the constituents present in Aloe vera. One of the major constituents is carbohydrate, which has not been explored much for its antioxidant and antiinflammatory activity. Here, we evaluated the antioxidant and anti-inflammatory potential of carbohydrate fraction (CF) of Aloe vera extract using in vitro and in vivo model for its therapeutic application in the treatment of inflammation. CF extract was prepared using hot water extraction followed by ethanolic precipitation. The CF extract was tested for free radical scavenging assays, lipopolysaccharide-stimulated RAW264.7 cells in vitro and in vivo by Freund's adjuvant induced rat paw edema model. The free radical scavenging activity of CF extract significantly increased in a concentration-dependent manner with maximum inhibition observed at the concentration of 2 mg/mL. CF extract (20 and 60 µg/mL) showed their maximum cell viability in RAW264.7 cells by MTT assay. CF extract shows a considerable decrease of inflammatory mediators in both in vitro and as well as in vivo studies. The increase of superoxide dismutase and catalase levels in CF treated adjuvant induced rats decreases ROS/RNS levels and inflammatory mediators. It may be primarily activated by NF-κB and AP-1 indicating its potent antioxidant activity that promotes modulation in cellular redox state. The results suggest that carbohydrate fraction of Aloe vera could be useful for the management of oxidative stress and inflammatory diseases.
A series of spiro-[indoline-3,3 0-pyrrolizin/pyrrolidin]-2-ones, 4, 5 and 6 were synthesized in a... more A series of spiro-[indoline-3,3 0-pyrrolizin/pyrrolidin]-2-ones, 4, 5 and 6 were synthesized in a sequential manner from Cu-TEMPO catalyzed dehydrogenation of alkylated ketones, 1 followed by 1,3-dipolar cycloaddition of azomethine ylides via decarboxylative condensation of isatin, 2 and L-proline/sarcosine, 3 in high regioselectivities and yields. The detailed mechanistic studies were performed to identify the reaction intermediates, which revealed that the reaction proceeds via dehydrogenative cycloaddition. Additionally, the regio and stereochemistry of the synthesized derivatives were affirmed by 2D NMR spectroscopic studies. The synthesized derivatives were explored further with molecular docking, in vitro antioxidant, and anti-diabetic activities.
Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia. Plant extracts ... more Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia. Plant extracts and their products are being used as an alternative system of medicine for the treatment of diabetes. Aloe vera has been traditionally used to treat several diseases and it exhibits antioxidant, anti-inflammatory, and wound-healing effects. Streptozotocin (STZ)-induced Wistar diabetic rats were used in this study to understand the potential protective effect of A. vera extract on the pancreatic islets. The aim of the present study was to evaluate the A. vera extract on improvement of insulin secretion and pancreatic β-cell function by morphometric analysis of pancreatic islets in STZ-induced diabetic Wistar rats. After acclimatization, male Wistar rats, maintained as per the Committee for the Purpose of Control and Supervision of Experiments on Animals guidelines, were randomly divided into four groups of six rats each. Fasting plasma glucose and insulin levels were assessed. The effect ...
International Journal of Phytomedicine, Sep 27, 2012
The context and purpose of the study: Plants that have been used for traditional medicines are ve... more The context and purpose of the study: Plants that have been used for traditional medicines are very good sources of phyotochemicals. There are many plants like Casuarina equisetifolia, which are still unexplored for their medicinal properties. In the present study, we have elucidated the in vitro antioxidant and antiproliferative activity of different solvent extracts (both polar and non polar) of C. equisetifolia needles. Main findings: In vitro antioxidant activity of different solvent extracts of C. equisetifolia needles was studied by analyzing the total polyphenols, flavonoids, total antioxidant capacity and free radical scavenging activity. The polar solvent extracts showed significantly high amount total polyphenols, flavonoids, antioxidants and free radical scavenging activity compared to the non polar solvent extracts. The cytotoxic and apoptosis inducing activity of the different solvent extracts were analyzed on MCF-7 cells by MTT assay, acridine orange/ethidium bromide staining, DAPI staining and caspase-3 release. The polar solvent extracts did not show any growth inhibition in MCF-7 cancer cells. But the non polar solvent extracts are very good in inducing cell death by inducing apoptosis which involves DNA fragmentation and release of caspase 3. Using silica gel fractionation and RP-HPLC analysis the active component present in non-polar solvent extracts was identified as ascorbic acid.
Résumé/Abstract An attempt was made to study the beneficial effects of Aloe vera (L.) Burm. fil. ... more Résumé/Abstract An attempt was made to study the beneficial effects of Aloe vera (L.) Burm. fil. in streptozotocin-induced diabetic rats. In diabetic induced rats fed with A. vera (300 mg/kg body wt), the fasting plasma glucose levels were reduced to normal and body ...
Although extensive literature exists on antioxidant properties of medicinal plants, very few stud... more Although extensive literature exists on antioxidant properties of medicinal plants, very few studies have focused on their polyphenolic composition. Here, preliminary phytochemical screening and total flavonoid content in extracts of seeds of Myristica fragrans and leaves of Cordyline terminalis have been investigated. Screening of potential flavonoid-subclasses was done by LC-ESI-MS, whose data were interpreted using database of Lipid Metabolites and Pathways Strategy Consortium. 'Flavones and Flavonols' and 'Anthocyanidins' appear to be more abundant in C. terminalis than in M. fragrans. Higher content of isoflavonoids, chalcones and dihydrochalcones and some isoprenoids were observed in M. fragrans than in C. terminalis. The strategy and results of this study will help to choose appropriate standards prior to their identification and confirmation in natural extracts. These results may also be useful to assess antioxidant activity of only the subclass alone and accordingly appropriate subclasses can be selected to prepare formulations having nutraceutical applications.
Although extensive literature exists on antioxidant properties of medicinal plants, very few stud... more Although extensive literature exists on antioxidant properties of medicinal plants, very few studies have focused on their polyphenolic composition. Here, preliminary phytochemical screening and total flavonoid content in extracts of seeds of Myristica fragrans and leaves of Cordyline terminalis have been investigated. Screening of potential flavonoid-subclasses was done by LC-ESI-MS, whose data were interpreted using database of Lipid Metabolites and Pathways Strategy Consortium. ‘Flavones and Flavonols’ and ‘Anthocyanidins’ appear to be more abundant in C. terminalis than in M. fragrans. Higher content of isoflavonoids, chalcones and dihydrochalcones and some isoprenoids were observed in M. fragrans than in C. terminalis. The strategy and results of this study will help to choose appropriate standards prior to their identification and confirmation in natural extracts. These results may also be useful to assess antioxidant activity of only the subclass alone and accordingly appropr...
Aloe vera (L.) Burm. F (Asphodelaceae) is a traditional medicinal plant having multiple biologica... more Aloe vera (L.) Burm. F (Asphodelaceae) is a traditional medicinal plant having multiple biological activities such as wound healing, anticancer and antidiabetic activity, etc. These properties have been attributed to the constituents present in Aloe vera. One of the major constituents is carbohydrate, which has not been explored much for its antioxidant and antiinflammatory activity. Here, we evaluated the antioxidant and anti-inflammatory potential of carbohydrate fraction (CF) of Aloe vera extract using in vitro and in vivo model for its therapeutic application in the treatment of inflammation. CF extract was prepared using hot water extraction followed by ethanolic precipitation. The CF extract was tested for free radical scavenging assays, lipopolysaccharide-stimulated RAW264.7 cells in vitro and in vivo by Freund's adjuvant induced rat paw edema model. The free radical scavenging activity of CF extract significantly increased in a concentration-dependent manner with maximum inhibition observed at the concentration of 2 mg/mL. CF extract (20 and 60 µg/mL) showed their maximum cell viability in RAW264.7 cells by MTT assay. CF extract shows a considerable decrease of inflammatory mediators in both in vitro and as well as in vivo studies. The increase of superoxide dismutase and catalase levels in CF treated adjuvant induced rats decreases ROS/RNS levels and inflammatory mediators. It may be primarily activated by NF-κB and AP-1 indicating its potent antioxidant activity that promotes modulation in cellular redox state. The results suggest that carbohydrate fraction of Aloe vera could be useful for the management of oxidative stress and inflammatory diseases.
A series of spiro-[indoline-3,3 0-pyrrolizin/pyrrolidin]-2-ones, 4, 5 and 6 were synthesized in a... more A series of spiro-[indoline-3,3 0-pyrrolizin/pyrrolidin]-2-ones, 4, 5 and 6 were synthesized in a sequential manner from Cu-TEMPO catalyzed dehydrogenation of alkylated ketones, 1 followed by 1,3-dipolar cycloaddition of azomethine ylides via decarboxylative condensation of isatin, 2 and L-proline/sarcosine, 3 in high regioselectivities and yields. The detailed mechanistic studies were performed to identify the reaction intermediates, which revealed that the reaction proceeds via dehydrogenative cycloaddition. Additionally, the regio and stereochemistry of the synthesized derivatives were affirmed by 2D NMR spectroscopic studies. The synthesized derivatives were explored further with molecular docking, in vitro antioxidant, and anti-diabetic activities.
Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia. Plant extracts ... more Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia. Plant extracts and their products are being used as an alternative system of medicine for the treatment of diabetes. Aloe vera has been traditionally used to treat several diseases and it exhibits antioxidant, anti-inflammatory, and wound-healing effects. Streptozotocin (STZ)-induced Wistar diabetic rats were used in this study to understand the potential protective effect of A. vera extract on the pancreatic islets. The aim of the present study was to evaluate the A. vera extract on improvement of insulin secretion and pancreatic β-cell function by morphometric analysis of pancreatic islets in STZ-induced diabetic Wistar rats. After acclimatization, male Wistar rats, maintained as per the Committee for the Purpose of Control and Supervision of Experiments on Animals guidelines, were randomly divided into four groups of six rats each. Fasting plasma glucose and insulin levels were assessed. The effect ...
International Journal of Phytomedicine, Sep 27, 2012
The context and purpose of the study: Plants that have been used for traditional medicines are ve... more The context and purpose of the study: Plants that have been used for traditional medicines are very good sources of phyotochemicals. There are many plants like Casuarina equisetifolia, which are still unexplored for their medicinal properties. In the present study, we have elucidated the in vitro antioxidant and antiproliferative activity of different solvent extracts (both polar and non polar) of C. equisetifolia needles. Main findings: In vitro antioxidant activity of different solvent extracts of C. equisetifolia needles was studied by analyzing the total polyphenols, flavonoids, total antioxidant capacity and free radical scavenging activity. The polar solvent extracts showed significantly high amount total polyphenols, flavonoids, antioxidants and free radical scavenging activity compared to the non polar solvent extracts. The cytotoxic and apoptosis inducing activity of the different solvent extracts were analyzed on MCF-7 cells by MTT assay, acridine orange/ethidium bromide staining, DAPI staining and caspase-3 release. The polar solvent extracts did not show any growth inhibition in MCF-7 cancer cells. But the non polar solvent extracts are very good in inducing cell death by inducing apoptosis which involves DNA fragmentation and release of caspase 3. Using silica gel fractionation and RP-HPLC analysis the active component present in non-polar solvent extracts was identified as ascorbic acid.
Résumé/Abstract An attempt was made to study the beneficial effects of Aloe vera (L.) Burm. fil. ... more Résumé/Abstract An attempt was made to study the beneficial effects of Aloe vera (L.) Burm. fil. in streptozotocin-induced diabetic rats. In diabetic induced rats fed with A. vera (300 mg/kg body wt), the fasting plasma glucose levels were reduced to normal and body ...
Although extensive literature exists on antioxidant properties of medicinal plants, very few stud... more Although extensive literature exists on antioxidant properties of medicinal plants, very few studies have focused on their polyphenolic composition. Here, preliminary phytochemical screening and total flavonoid content in extracts of seeds of Myristica fragrans and leaves of Cordyline terminalis have been investigated. Screening of potential flavonoid-subclasses was done by LC-ESI-MS, whose data were interpreted using database of Lipid Metabolites and Pathways Strategy Consortium. 'Flavones and Flavonols' and 'Anthocyanidins' appear to be more abundant in C. terminalis than in M. fragrans. Higher content of isoflavonoids, chalcones and dihydrochalcones and some isoprenoids were observed in M. fragrans than in C. terminalis. The strategy and results of this study will help to choose appropriate standards prior to their identification and confirmation in natural extracts. These results may also be useful to assess antioxidant activity of only the subclass alone and accordingly appropriate subclasses can be selected to prepare formulations having nutraceutical applications.
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