Objective: The present study was carried out to evaluate the biological active phytochemical comp... more Objective: The present study was carried out to evaluate the biological active phytochemical compounds from moss of Berijam Lake, Kodaikanal, India. Methods: Acetone and methanol extracts of moss were obtained using soxhlet extractor and the obtained extracts were evaluated for various biological activities. The combined effect of commercially available antibiotics and moss extracts was also investigated by disk-diffusion assay method against various Gram positive and Gram negative bacteria. The free radical scavenging activity of moss extracts were studied by 2,2-diphenyl-1-picrylhydrazyl (DPPH). Results: The maximum zone of inhibition was found against Gram negative pathogens whereas weak inhibitory activity was investigated against Staphylococcus aureus. The combined effect of chloramphenicol with acetone and methanol extracts exhibited pronounced antimicrobial effect against Bacillus subtilis, Proteus mirabilis and Escherichia coli. The phytochemical tests revealed the presence of carbohydrates in Funaria sp. and the acetone extract showed effective antioxidant activity when compare to methanol extract using free radical scavenging assay. Conclusion: The present study reveals that the bryophytes from Berijam Lake possess potential antimicrobial and antioxidant activity.
The microbiota of the human large intestine influences health and well-being. Whereas it has long... more The microbiota of the human large intestine influences health and well-being. Whereas it has long been accepted that gut bacteria play a role in host pathogenesis, current opinion is that certain microflora components can have beneficial effects on gastroenteritis resistance, ...
International Journal of Pharmaceutical Sciences Review and Research
Amycolatopsis decaplanina JAR8 is rare actinomycetes which was isolated from agricultural field o... more Amycolatopsis decaplanina JAR8 is rare actinomycetes which was isolated from agricultural field of Punjab, India. The 16S rRNA gene sequencing of strain JAR8 showed 98% similarity with Amycolatopsis decaplanina. The morphological, cultural and physiological characteristics were determined by International Streptomyces project by using various mediums. The bioactive metabolites produced by the strain were obtained from the optimized culture medium through solvent extraction method. The ethyl acetate extract obtained after extraction was characterized using UV, FTIR and GC-MS spectroscopic analysis. The minimum inhibitory concentration of active metabolite was found to be 60 µg/ml against Staphylococcus aureus. The present study deals with the exploration of rare actinomycetes which also produces various biological active compounds against infectious diseases.
The present investigation reveals the green synthesis of silver nanoparticles using Solanum nigru... more The present investigation reveals the green synthesis of silver nanoparticles using Solanum nigrum and Cardiospermum halicacabum. The silver nanoparticles were synthesized and characterized by UV-Vis spectrophotometer (UV-Vis), Fourier transfer infrared spectrophotometer (FT-IR), X-ray diffraction (XRD), atomic force mass spectroscopy (AFM) and particle shape of the synthesized nanoparticles were determined by scanning electron microscopy (SEM). Furthermore, synthesized nanoparticles were evaluated for antimicrobial effect against various pathogens and cytotoxic effect on human osteosarcoma (MG-63) cell line. The plant mediated silver nanoparticles exhibited good antimicrobial activity against clinical pathogens and cytotoxic effect against bone cancer cells. Plant mediated silver nanoparticles possess therapeutic properties which may be useful in pharmaceutical applications.
International Journal of Pharmaceutical Sciences and Research
Actinomycetes are versatile and prolific source of microbial natural products with great advantag... more Actinomycetes are versatile and prolific source of microbial natural products with great advantage in medicine and agriculture. Recently there is growing interest in exploring biological active compounds from rare actinomycetes. Usually rare actinomycetes are regarded as strains of actinomycetes whose isolation frequency by conventional methods is much lower than that of Streptomycetes strains. Amycolatopsis orientalis JAR10 rare genera of actinomycetes which have been isolated, characterized and antimicrobial activity against various pathogens have been determined in the present work. The morphological, cultural, genotypical and physiological characteristics of strain JAR10 has been examined by International Streptomyces Project. The bioactive metabolites produced by strain JAR10 were extracted by solvent extraction method and the crude extract obtained was further analyzed against various pathogens. The minimum inhibitory concentration of crude extract obtained from Amycolatopsis ...
International Journal of Pharmaceutical Sciences and Research
ABSTRACT: In the present study, Vigna mungo (blackgram) have been used to investigate bioactive c... more ABSTRACT: In the present study, Vigna mungo (blackgram) have been used to investigate bioactive compounds through soxhlet extraction. The obtained methanol extract was evaluated for antimicrobial activity by Kirby- Bauer method against bacterial strains (Enterococci sp., Pseudomonas aeruginosa, Klebsiella sp., Bacillus sp., Staphylococcus aueres) and fungal strains (Aspergillus niger, Candida albicans) on Muller Hinton agar medium and potato dextrose agar medium respectively. The blackgram was analyzed for the presence of proteins through SDS-PAGE and compared with the BSA (66KDa) and Lysine (18KDa) protein markers. The sensitivity of the obtained methanol extract against pathogenic microorganisms was studied with the combined effect of commercially available antibiotics which resulted in good and enhanced antimicrobial effect. The methanol extract of blackgram was also analyzed for bioactive compounds through gaschromatography mass spectrometry (GC-MS). The GC-MS spectrum revealed ...
The ongoing increasing antibiotic resistance is one of the biggest challenges faced by global pub... more The ongoing increasing antibiotic resistance is one of the biggest challenges faced by global public health. The perennial need for new antimicrobials against a background of increasing antibiotic resistance in pathogenic and opportunistic microorganisms obliges the scientific community to constantly develop new drugs and antimicrobial agents. Lichens are known prolific sources of natural antimicrobial drugs and biologically active natural products. This study was aimed to explore in vitro antimicrobial activity of lichen Parmotrema sp. The methanol and aqueous extracts of lichen Parmotrema sp. was extracted using Soxhlet extractor. Antibiotic assessment of methanol and aqueous extracts was done against eight bacterial (Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Salmonella sp., Shigella sp., Enterococci faecalis, Pseudomonas aeruginosa, Klebsiella pneumoniae,) clinical pathogens and five plant pathogenic fungal strains (Aspergillus terreus strain JAS1, Scedosporium ...
The biological approach to synthesize metal nanoparticles is an important aspect of current nanot... more The biological approach to synthesize metal nanoparticles is an important aspect of current nanotechnology research. Silver nanoparticles have been well-known for their inhibitory and antimicrobial effects. The ever-increasing antibiotic resistance in pathogenic and opportunistic microorganisms is a major threat to the health care industry. In the present investigation, silver nanoparticles have been successfully biosynthesized by Streptomyces sp JAR1. Biosynthesized silver nanoparticles were characterized by means of several analytical techniques including a UV-Visible spectrophotometer, Fourier transform infrared spectroscopy, X-ray diffraction pattern analysis, and atomic force microscopy. An evaluation of the antimicrobial activity of silver nanoparticles (AgNPs) was carried out against clinically important pathogenic microorganisms. The metal nanoparticles were also evaluated for their combined effects with antibiotics against the clinical pathogens. The anti-bacterial activities of the antibiotics increased in the presence of the biologically synthesized AgNPs against the clinically important pathogens. The highest enhancing effect was observed for erythromycin against the test pathogens.
Objective: The present study was carried out to evaluate the biological active phytochemical comp... more Objective: The present study was carried out to evaluate the biological active phytochemical compounds from moss of Berijam Lake, Kodaikanal, India. Methods: Acetone and methanol extracts of moss were obtained using soxhlet extractor and the obtained extracts were evaluated for various biological activities. The combined effect of commercially available antibiotics and moss extracts was also investigated by disk-diffusion assay method against various Gram positive and Gram negative bacteria. The free radical scavenging activity of moss extracts were studied by 2,2-diphenyl-1-picrylhydrazyl (DPPH). Results: The maximum zone of inhibition was found against Gram negative pathogens whereas weak inhibitory activity was investigated against Staphylococcus aureus. The combined effect of chloramphenicol with acetone and methanol extracts exhibited pronounced antimicrobial effect against Bacillus subtilis, Proteus mirabilis and Escherichia coli. The phytochemical tests revealed the presence of carbohydrates in Funaria sp. and the acetone extract showed effective antioxidant activity when compare to methanol extract using free radical scavenging assay. Conclusion: The present study reveals that the bryophytes from Berijam Lake possess potential antimicrobial and antioxidant activity.
The microbiota of the human large intestine influences health and well-being. Whereas it has long... more The microbiota of the human large intestine influences health and well-being. Whereas it has long been accepted that gut bacteria play a role in host pathogenesis, current opinion is that certain microflora components can have beneficial effects on gastroenteritis resistance, ...
International Journal of Pharmaceutical Sciences Review and Research
Amycolatopsis decaplanina JAR8 is rare actinomycetes which was isolated from agricultural field o... more Amycolatopsis decaplanina JAR8 is rare actinomycetes which was isolated from agricultural field of Punjab, India. The 16S rRNA gene sequencing of strain JAR8 showed 98% similarity with Amycolatopsis decaplanina. The morphological, cultural and physiological characteristics were determined by International Streptomyces project by using various mediums. The bioactive metabolites produced by the strain were obtained from the optimized culture medium through solvent extraction method. The ethyl acetate extract obtained after extraction was characterized using UV, FTIR and GC-MS spectroscopic analysis. The minimum inhibitory concentration of active metabolite was found to be 60 µg/ml against Staphylococcus aureus. The present study deals with the exploration of rare actinomycetes which also produces various biological active compounds against infectious diseases.
The present investigation reveals the green synthesis of silver nanoparticles using Solanum nigru... more The present investigation reveals the green synthesis of silver nanoparticles using Solanum nigrum and Cardiospermum halicacabum. The silver nanoparticles were synthesized and characterized by UV-Vis spectrophotometer (UV-Vis), Fourier transfer infrared spectrophotometer (FT-IR), X-ray diffraction (XRD), atomic force mass spectroscopy (AFM) and particle shape of the synthesized nanoparticles were determined by scanning electron microscopy (SEM). Furthermore, synthesized nanoparticles were evaluated for antimicrobial effect against various pathogens and cytotoxic effect on human osteosarcoma (MG-63) cell line. The plant mediated silver nanoparticles exhibited good antimicrobial activity against clinical pathogens and cytotoxic effect against bone cancer cells. Plant mediated silver nanoparticles possess therapeutic properties which may be useful in pharmaceutical applications.
International Journal of Pharmaceutical Sciences and Research
Actinomycetes are versatile and prolific source of microbial natural products with great advantag... more Actinomycetes are versatile and prolific source of microbial natural products with great advantage in medicine and agriculture. Recently there is growing interest in exploring biological active compounds from rare actinomycetes. Usually rare actinomycetes are regarded as strains of actinomycetes whose isolation frequency by conventional methods is much lower than that of Streptomycetes strains. Amycolatopsis orientalis JAR10 rare genera of actinomycetes which have been isolated, characterized and antimicrobial activity against various pathogens have been determined in the present work. The morphological, cultural, genotypical and physiological characteristics of strain JAR10 has been examined by International Streptomyces Project. The bioactive metabolites produced by strain JAR10 were extracted by solvent extraction method and the crude extract obtained was further analyzed against various pathogens. The minimum inhibitory concentration of crude extract obtained from Amycolatopsis ...
International Journal of Pharmaceutical Sciences and Research
ABSTRACT: In the present study, Vigna mungo (blackgram) have been used to investigate bioactive c... more ABSTRACT: In the present study, Vigna mungo (blackgram) have been used to investigate bioactive compounds through soxhlet extraction. The obtained methanol extract was evaluated for antimicrobial activity by Kirby- Bauer method against bacterial strains (Enterococci sp., Pseudomonas aeruginosa, Klebsiella sp., Bacillus sp., Staphylococcus aueres) and fungal strains (Aspergillus niger, Candida albicans) on Muller Hinton agar medium and potato dextrose agar medium respectively. The blackgram was analyzed for the presence of proteins through SDS-PAGE and compared with the BSA (66KDa) and Lysine (18KDa) protein markers. The sensitivity of the obtained methanol extract against pathogenic microorganisms was studied with the combined effect of commercially available antibiotics which resulted in good and enhanced antimicrobial effect. The methanol extract of blackgram was also analyzed for bioactive compounds through gaschromatography mass spectrometry (GC-MS). The GC-MS spectrum revealed ...
The ongoing increasing antibiotic resistance is one of the biggest challenges faced by global pub... more The ongoing increasing antibiotic resistance is one of the biggest challenges faced by global public health. The perennial need for new antimicrobials against a background of increasing antibiotic resistance in pathogenic and opportunistic microorganisms obliges the scientific community to constantly develop new drugs and antimicrobial agents. Lichens are known prolific sources of natural antimicrobial drugs and biologically active natural products. This study was aimed to explore in vitro antimicrobial activity of lichen Parmotrema sp. The methanol and aqueous extracts of lichen Parmotrema sp. was extracted using Soxhlet extractor. Antibiotic assessment of methanol and aqueous extracts was done against eight bacterial (Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Salmonella sp., Shigella sp., Enterococci faecalis, Pseudomonas aeruginosa, Klebsiella pneumoniae,) clinical pathogens and five plant pathogenic fungal strains (Aspergillus terreus strain JAS1, Scedosporium ...
The biological approach to synthesize metal nanoparticles is an important aspect of current nanot... more The biological approach to synthesize metal nanoparticles is an important aspect of current nanotechnology research. Silver nanoparticles have been well-known for their inhibitory and antimicrobial effects. The ever-increasing antibiotic resistance in pathogenic and opportunistic microorganisms is a major threat to the health care industry. In the present investigation, silver nanoparticles have been successfully biosynthesized by Streptomyces sp JAR1. Biosynthesized silver nanoparticles were characterized by means of several analytical techniques including a UV-Visible spectrophotometer, Fourier transform infrared spectroscopy, X-ray diffraction pattern analysis, and atomic force microscopy. An evaluation of the antimicrobial activity of silver nanoparticles (AgNPs) was carried out against clinically important pathogenic microorganisms. The metal nanoparticles were also evaluated for their combined effects with antibiotics against the clinical pathogens. The anti-bacterial activities of the antibiotics increased in the presence of the biologically synthesized AgNPs against the clinically important pathogens. The highest enhancing effect was observed for erythromycin against the test pathogens.
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