International Journal of Zoology and Applied Biosciences, 2022
In human female reproductive physiology predicting ovulation is the basis on which the fertile pe... more In human female reproductive physiology predicting ovulation is the basis on which the fertile period is determined. The present study was designed to investigate the level of salivary Sialic acid to find the possibility to develop a biomarker for detection of ovulation by non-invasive method. 42 females of different age group of different reproductive physiological conditions (i.e. prepubertal, parous, non-parous, menopausal, and diabetogenic) were selected for this study. It was observed significant (p<0.001) increase in ovulatory, menopause and diabetic condition and highly significant decrease in non-parous ovulatory and post ovulatory condition in women. The study showed that salivary Sialic acid concentration was elevated during ovulation.
International Journal of Agricultural and Applied Sciences, 2022
In the present study, four plant extracts (Allium sativum L., Desmodium gangeticum L., Eclipta al... more In the present study, four plant extracts (Allium sativum L., Desmodium gangeticum L., Eclipta alba L., and Piper longum L.) were considered and checked for their acetylcholinesterase inhibitory activity which is the main true enzyme which hydrolyses acetylcholine in the body. The dried coarse powder of plants was extracted with methanol by cold extraction method. The resultant was assessed for acetylcholinesterase (AChE) inhibitory activity by Ellman's method with few modifications. The antioxidant activity was determined by DPPH (1, 1-diphenyl-2-picrylhydrazyl) and FRAP (Ferrous reducing Antioxidant power) assays. Quantitative phytochemical (phenolic contents) analysis of endogenous substances was performed by standard spectrophotometric methods. Plant extract significantly inhibited AChE activity. Additionally, the plant extracts exhibited strong radical scavenging activity against DPPH and reduced the Ferric ion (FRAP) significantly when compared to that of standards. Plant extracts were found to be rich in phenolic (gallic acid equivalent/g of dry extract) content. Furthermore, a positive correlation was observed between the total phenolics and antioxidants as well as the anticholinesterase potential.
Saliva is a complex diagnostic fluid, which contains a variety of enzymes, hormones, antibodies, ... more Saliva is a complex diagnostic fluid, which contains a variety of enzymes, hormones, antibodies, antimicrobial constituents and growth factors. In our body fluids like blood, saliva, tears, sweat and urine are crucial resources of pathological diagnosis. Changing in level of total cholesterol level in human female saliva was investigated in 60 women volunteers during various stages of reproduction (pre-pubertal, parous, nonparous, menopausal and diabetogenic). According to findings, level of total cholesterol level is significantly increased during ovulatory phase, which was due to hormonal metabolic changes in the period of menstrual cycle.
Saliva has been studied extensively as a potential diagnostic tool over the last decade due to it... more Saliva has been studied extensively as a potential diagnostic tool over the last decade due to its ease and non-invasive accessibility along with its abundance of biomarkers, such as genetic material and proteins.The activity of protein in saliva increased during ovulation. When we monitored salivary protein activity in 40 different women volunteers during various stages of reproduction like (prepubertal, parous, non-parous, menopausal and in the metabolic disorder state diabetic condition it has been observed that highly significant (p<0.001) increase in parous ovulatory & non parous ovulatory whereas, highly significant (p<0.01)increase was observed in non-parous postovulatory and a highly significant (p<0.001) decrease was observed in menopause and diabetic in comparison to prepubertal. A highly significant (p<0.001) decrease was observed in menopause in comparison to parous preovulatory, ovulatory, post ovulatory & non-parous ovulatory and postovulatory. A highly sig...
International Journal of Zoology and Applied Biosciences, 2022
In human female reproductive physiology predicting ovulation is the basis on which the fertile pe... more In human female reproductive physiology predicting ovulation is the basis on which the fertile period is determined. The present study was designed to investigate the level of salivary Sialic acid to find the possibility to develop a biomarker for detection of ovulation by non-invasive method. 42 females of different age group of different reproductive physiological conditions (i.e. prepubertal, parous, non-parous, menopausal, and diabetogenic) were selected for this study. It was observed significant (p<0.001) increase in ovulatory, menopause and diabetic condition and highly significant decrease in non-parous ovulatory and post ovulatory condition in women. The study showed that salivary Sialic acid concentration was elevated during ovulation.
International Journal of Agricultural and Applied Sciences, 2022
In the present study, four plant extracts (Allium sativum L., Desmodium gangeticum L., Eclipta al... more In the present study, four plant extracts (Allium sativum L., Desmodium gangeticum L., Eclipta alba L., and Piper longum L.) were considered and checked for their acetylcholinesterase inhibitory activity which is the main true enzyme which hydrolyses acetylcholine in the body. The dried coarse powder of plants was extracted with methanol by cold extraction method. The resultant was assessed for acetylcholinesterase (AChE) inhibitory activity by Ellman's method with few modifications. The antioxidant activity was determined by DPPH (1, 1-diphenyl-2-picrylhydrazyl) and FRAP (Ferrous reducing Antioxidant power) assays. Quantitative phytochemical (phenolic contents) analysis of endogenous substances was performed by standard spectrophotometric methods. Plant extract significantly inhibited AChE activity. Additionally, the plant extracts exhibited strong radical scavenging activity against DPPH and reduced the Ferric ion (FRAP) significantly when compared to that of standards. Plant extracts were found to be rich in phenolic (gallic acid equivalent/g of dry extract) content. Furthermore, a positive correlation was observed between the total phenolics and antioxidants as well as the anticholinesterase potential.
Saliva has been studied extensively as a potential diagnostic tool over the last decade due to it... more Saliva has been studied extensively as a potential diagnostic tool over the last decade due to its ease and non-invasive accessibility along with its abundance of biomarkers, such as genetic material and proteins. The activity of protein in saliva increased during ovulation. When we monitored salivary protein activity in 40 different women volunteers during various stages of reproduction like (prepubertal, parous, non-parous, menopausal and in the metabolic disorder state diabetic condition it has been observed that highly significant (p<0.001) increase in parous ovulatory & non parous ovulatory whereas, highly significant (p<0.01)increase was observed in non-parous postovulatory and a highly significant (p<0.001) decrease was observed in menopause and diabetic in comparison to prepubertal. A highly significant (p<0.001) decrease was observed in menopause in comparison to parous preovulatory, ovulatory, post ovulatory & non-parous ovulatory and postovulatory. A highly significant (p<0.01) decrease was observed in diabetic in comparison to menopausal human female subjects. The result revealed that the total protein was considered as testing the saliva instead of blood isa non-invasive loom and it can be used as a biomarker for ovulation detection.
The methanolic extracts of Allium sativum were investigated for its antioxidant activity by using... more The methanolic extracts of Allium sativum were investigated for its antioxidant activity by using 1,1-diphenyl-2- picryl hydrazyl (DPPH) radical scavenging assay and by ferric reducing antioxidant power (FRAP) assay. The total phenolic content was evaluated by Folin-Ciocalteu procedure. This study indicated that Allium sativum L. exhibited the high antioxidant activity and phenolic contents and can be used potentially as a readily accessible source of natural antioxidant. Due to its natural origin and potent free-radical scavenging ability, Allium sativum L. could be used as a potential preventive intervention for free radical-mediated diseases.
Acetylcholinesterase (AChE), the predominant cholinesterase in the brain, hydrolyzes ACh to choli... more Acetylcholinesterase (AChE), the predominant cholinesterase in the brain, hydrolyzes ACh to choline and acetate, thereby terminating the effect of this neurotransmitter at cholinergic synapses. Therefore, AChE is the target of cholinesterase inhibitors used for addressing the cholinergic deficit in Alzheimer’s disease (AD) patients. Despite decades of research and advances in our understanding of its aetiology and pathogenesis, current pharmacotherapeutic options for AD are still very limited and represent an area of need that is currently unmet. The leading AD therapeutics involves AChE inhibitors, resulting in increased acetylcholine concentrations in the synaptic cleft and enhanced cholinergic transmission. Compounds showing an AChE inhibitory effect are also used for the treatment of senile dementia, myastenia gravis, Parkinson’s disease and ataxia. Taking into account that the inhibition of AChE has been one of the most used strategies for treating AD and that existing drugs ar...
Acetylcholinesterase (AChE) inhibition and antioxidant activity are considered to be highly corre... more Acetylcholinesterase (AChE) inhibition and antioxidant activity are considered to be highly correlated with Alzheimer’s disease (AD) treatment. The present study was designed to investigate the antioxidant and acetylcholinesterase inhibitory activity of Piper longum L. Properly identified powdered plant material was extracted successively using methanol as a solvent. Acetylcholinesterase inhibitory activity was measured with modified Ellman’s method at 405 nm and antioxidant activity measured based on 1, 1- diphenyl-2-picrylhydrazil (DPPH) free radical scavenging test at 517 nm. Percentage inhibition for AChE ranged from 31.28±0.12 to 62.26±0.05 whereas DPPH radical scavenging percentage ranged from 14.40±0.72 to 37.95±0.67. Results reveal that plant extracts studied possess anti-oxidant properties. Most potent extracts could be a lead to novel antioxidants and acetylcholinesterase inhibitors for the treatment of AD.
Acetylcholinesterase (AChE) inhibition and antioxidant activity are considered to be highly corre... more Acetylcholinesterase (AChE) inhibition and antioxidant activity are considered to be highly correlated with Alzheimer’s disease treatment. The present study was designed to investigate the antioxidant and acetylcholinesterase inhibitory activity of Desmodium gangeticum L. Properly identified powdered plant material was extracted successively using methanol as a solvent. Acetylcholinesterase inhibitory activity was measured with modified Ellman method at 405 nm and antioxidant activity measured based on 1, 1- diphenyl-2-picrylhydrazil (DPPH) free radical scavenging test at 517 nm. Percentage inhibition for AChE ranged from 28.78±0.12 to 40.83±0.05 whereas DPPH radical scavenging percentage ranged from 24.68±0.72 to 42.22±0.67.
Acetylcholinesterase (AChE) inhibition and antioxidant activity are considered to be highly corre... more Acetylcholinesterase (AChE) inhibition and antioxidant activity are considered to be highly correlated with Alzheimer’s disease treatment. The present study was designed to investigate the antioxidant and acetylcholinesterase inhibitory activity of Desmodium gangeticum L. Properly identified powdered plant material was extracted successively using methanol as a solvent. Acetylcholinesterase inhibitory activity was measured with modified Ellman method at 405 nm and antioxidant activity measured based on 1, 1- diphenyl-2-picrylhydrazil (DPPH) free radical scavenging test at 517 nm. Percentage inhibition for AChE ranged from 28.78±0.12 to 40.83±0.05 whereas DPPH radical scavenging percentage ranged from 24.68±0.72 to 42.22±0.67.
Acetylcholinesterase (AChE), the predominant cholinesterase in the brain, hydrolyzes ACh to choli... more Acetylcholinesterase (AChE), the predominant cholinesterase in the brain, hydrolyzes ACh to choline and acetate, thereby terminating the effect of this neurotransmitter at cholinergic synapses. Therefore, AChE is the target of cholinesterase inhibitors used for addressing the cholinergic deficit in Alzheimer's disease (AD) patients. Despite decades of research and advances in our understanding of its aetiology and pathogenesis, current pharmacotherapeutic options for AD are still very limited and represent an area of need that is currently unmet. The leading AD therapeutics involves AChE inhibitors, resulting in increased acetylcholine concentrations in the synaptic cleft and enhanced cholinergic transmission. Compounds showing an AChE inhibitory effect are also used for the treatment of senile dementia, myastenia gravis, Parkinson's disease and ataxia. Taking into account that the inhibition of AChE has been one of the most used strategies for treating AD and that existing drugs are effective only against mild to moderate type of disease while presenting considerable side effects, the search for new sources of effective and selective anti acetylcholinesterase agents with fewer side effects is imperative. Various plants and phytochemical substances have demonstrated AChE inhibitory activity and thus could be beneficial in the treatment of neurodegenerative disorders such as AD.
The methanolic extracts of Allium sativum were investigated for its antioxidant activity by using... more The methanolic extracts of Allium sativum were investigated for its antioxidant activity by using 1,1-diphenyl-2- picryl hydrazyl (DPPH) radical scavenging assay and by ferric reducing antioxidant power (FRAP) assay. The total
phenolic content was evaluated by Folin-Ciocalteu procedure. This study indicated that Allium sativum L. exhibited the
high antioxidant activity and phenolic contents andcan be used potentially as a readily accessible source of natural
antioxidant. Due to its natural origin and potent free-radical scavenging ability, Allium sativum L. could be used as a
potential preventive intervention for free radical-mediated diseases.
Keywords: Allium sativum L., Antioxidant activity, DPPH, FRAP, Phenolic content.
In the present work an attempt has been made to compare the in vitro antioxidant activities of cr... more In the present work an attempt has been made to compare the in vitro antioxidant activities of crude extracts O.sanctum using Water, Methanol and Ethanol as solvent by DPPH scavenging assay. Ethanolic extract gave maximum yield of crude extract as well as antioxidant activity. The analyzed plant extracts showed the scavenging of DPPH radicals in the range from 58.45±0.2 to 92.82±0.5. These values are comparable with Ascorbic acid as standard. These findings suggest that the rich phytochemical content of O.sanctum and its good antioxidant activity may be responsible for its popular and wide traditional use.
KEY WORDS: O. sanctum, DPPH, Antioxidant activity, ascorbic acid, Free radical.
International Journal of Zoology and Applied Biosciences, 2022
In human female reproductive physiology predicting ovulation is the basis on which the fertile pe... more In human female reproductive physiology predicting ovulation is the basis on which the fertile period is determined. The present study was designed to investigate the level of salivary Sialic acid to find the possibility to develop a biomarker for detection of ovulation by non-invasive method. 42 females of different age group of different reproductive physiological conditions (i.e. prepubertal, parous, non-parous, menopausal, and diabetogenic) were selected for this study. It was observed significant (p<0.001) increase in ovulatory, menopause and diabetic condition and highly significant decrease in non-parous ovulatory and post ovulatory condition in women. The study showed that salivary Sialic acid concentration was elevated during ovulation.
International Journal of Agricultural and Applied Sciences, 2022
In the present study, four plant extracts (Allium sativum L., Desmodium gangeticum L., Eclipta al... more In the present study, four plant extracts (Allium sativum L., Desmodium gangeticum L., Eclipta alba L., and Piper longum L.) were considered and checked for their acetylcholinesterase inhibitory activity which is the main true enzyme which hydrolyses acetylcholine in the body. The dried coarse powder of plants was extracted with methanol by cold extraction method. The resultant was assessed for acetylcholinesterase (AChE) inhibitory activity by Ellman's method with few modifications. The antioxidant activity was determined by DPPH (1, 1-diphenyl-2-picrylhydrazyl) and FRAP (Ferrous reducing Antioxidant power) assays. Quantitative phytochemical (phenolic contents) analysis of endogenous substances was performed by standard spectrophotometric methods. Plant extract significantly inhibited AChE activity. Additionally, the plant extracts exhibited strong radical scavenging activity against DPPH and reduced the Ferric ion (FRAP) significantly when compared to that of standards. Plant extracts were found to be rich in phenolic (gallic acid equivalent/g of dry extract) content. Furthermore, a positive correlation was observed between the total phenolics and antioxidants as well as the anticholinesterase potential.
Saliva is a complex diagnostic fluid, which contains a variety of enzymes, hormones, antibodies, ... more Saliva is a complex diagnostic fluid, which contains a variety of enzymes, hormones, antibodies, antimicrobial constituents and growth factors. In our body fluids like blood, saliva, tears, sweat and urine are crucial resources of pathological diagnosis. Changing in level of total cholesterol level in human female saliva was investigated in 60 women volunteers during various stages of reproduction (pre-pubertal, parous, nonparous, menopausal and diabetogenic). According to findings, level of total cholesterol level is significantly increased during ovulatory phase, which was due to hormonal metabolic changes in the period of menstrual cycle.
Saliva has been studied extensively as a potential diagnostic tool over the last decade due to it... more Saliva has been studied extensively as a potential diagnostic tool over the last decade due to its ease and non-invasive accessibility along with its abundance of biomarkers, such as genetic material and proteins.The activity of protein in saliva increased during ovulation. When we monitored salivary protein activity in 40 different women volunteers during various stages of reproduction like (prepubertal, parous, non-parous, menopausal and in the metabolic disorder state diabetic condition it has been observed that highly significant (p<0.001) increase in parous ovulatory & non parous ovulatory whereas, highly significant (p<0.01)increase was observed in non-parous postovulatory and a highly significant (p<0.001) decrease was observed in menopause and diabetic in comparison to prepubertal. A highly significant (p<0.001) decrease was observed in menopause in comparison to parous preovulatory, ovulatory, post ovulatory & non-parous ovulatory and postovulatory. A highly sig...
International Journal of Zoology and Applied Biosciences, 2022
In human female reproductive physiology predicting ovulation is the basis on which the fertile pe... more In human female reproductive physiology predicting ovulation is the basis on which the fertile period is determined. The present study was designed to investigate the level of salivary Sialic acid to find the possibility to develop a biomarker for detection of ovulation by non-invasive method. 42 females of different age group of different reproductive physiological conditions (i.e. prepubertal, parous, non-parous, menopausal, and diabetogenic) were selected for this study. It was observed significant (p<0.001) increase in ovulatory, menopause and diabetic condition and highly significant decrease in non-parous ovulatory and post ovulatory condition in women. The study showed that salivary Sialic acid concentration was elevated during ovulation.
International Journal of Agricultural and Applied Sciences, 2022
In the present study, four plant extracts (Allium sativum L., Desmodium gangeticum L., Eclipta al... more In the present study, four plant extracts (Allium sativum L., Desmodium gangeticum L., Eclipta alba L., and Piper longum L.) were considered and checked for their acetylcholinesterase inhibitory activity which is the main true enzyme which hydrolyses acetylcholine in the body. The dried coarse powder of plants was extracted with methanol by cold extraction method. The resultant was assessed for acetylcholinesterase (AChE) inhibitory activity by Ellman's method with few modifications. The antioxidant activity was determined by DPPH (1, 1-diphenyl-2-picrylhydrazyl) and FRAP (Ferrous reducing Antioxidant power) assays. Quantitative phytochemical (phenolic contents) analysis of endogenous substances was performed by standard spectrophotometric methods. Plant extract significantly inhibited AChE activity. Additionally, the plant extracts exhibited strong radical scavenging activity against DPPH and reduced the Ferric ion (FRAP) significantly when compared to that of standards. Plant extracts were found to be rich in phenolic (gallic acid equivalent/g of dry extract) content. Furthermore, a positive correlation was observed between the total phenolics and antioxidants as well as the anticholinesterase potential.
Saliva has been studied extensively as a potential diagnostic tool over the last decade due to it... more Saliva has been studied extensively as a potential diagnostic tool over the last decade due to its ease and non-invasive accessibility along with its abundance of biomarkers, such as genetic material and proteins. The activity of protein in saliva increased during ovulation. When we monitored salivary protein activity in 40 different women volunteers during various stages of reproduction like (prepubertal, parous, non-parous, menopausal and in the metabolic disorder state diabetic condition it has been observed that highly significant (p<0.001) increase in parous ovulatory & non parous ovulatory whereas, highly significant (p<0.01)increase was observed in non-parous postovulatory and a highly significant (p<0.001) decrease was observed in menopause and diabetic in comparison to prepubertal. A highly significant (p<0.001) decrease was observed in menopause in comparison to parous preovulatory, ovulatory, post ovulatory & non-parous ovulatory and postovulatory. A highly significant (p<0.01) decrease was observed in diabetic in comparison to menopausal human female subjects. The result revealed that the total protein was considered as testing the saliva instead of blood isa non-invasive loom and it can be used as a biomarker for ovulation detection.
The methanolic extracts of Allium sativum were investigated for its antioxidant activity by using... more The methanolic extracts of Allium sativum were investigated for its antioxidant activity by using 1,1-diphenyl-2- picryl hydrazyl (DPPH) radical scavenging assay and by ferric reducing antioxidant power (FRAP) assay. The total phenolic content was evaluated by Folin-Ciocalteu procedure. This study indicated that Allium sativum L. exhibited the high antioxidant activity and phenolic contents and can be used potentially as a readily accessible source of natural antioxidant. Due to its natural origin and potent free-radical scavenging ability, Allium sativum L. could be used as a potential preventive intervention for free radical-mediated diseases.
Acetylcholinesterase (AChE), the predominant cholinesterase in the brain, hydrolyzes ACh to choli... more Acetylcholinesterase (AChE), the predominant cholinesterase in the brain, hydrolyzes ACh to choline and acetate, thereby terminating the effect of this neurotransmitter at cholinergic synapses. Therefore, AChE is the target of cholinesterase inhibitors used for addressing the cholinergic deficit in Alzheimer’s disease (AD) patients. Despite decades of research and advances in our understanding of its aetiology and pathogenesis, current pharmacotherapeutic options for AD are still very limited and represent an area of need that is currently unmet. The leading AD therapeutics involves AChE inhibitors, resulting in increased acetylcholine concentrations in the synaptic cleft and enhanced cholinergic transmission. Compounds showing an AChE inhibitory effect are also used for the treatment of senile dementia, myastenia gravis, Parkinson’s disease and ataxia. Taking into account that the inhibition of AChE has been one of the most used strategies for treating AD and that existing drugs ar...
Acetylcholinesterase (AChE) inhibition and antioxidant activity are considered to be highly corre... more Acetylcholinesterase (AChE) inhibition and antioxidant activity are considered to be highly correlated with Alzheimer’s disease (AD) treatment. The present study was designed to investigate the antioxidant and acetylcholinesterase inhibitory activity of Piper longum L. Properly identified powdered plant material was extracted successively using methanol as a solvent. Acetylcholinesterase inhibitory activity was measured with modified Ellman’s method at 405 nm and antioxidant activity measured based on 1, 1- diphenyl-2-picrylhydrazil (DPPH) free radical scavenging test at 517 nm. Percentage inhibition for AChE ranged from 31.28±0.12 to 62.26±0.05 whereas DPPH radical scavenging percentage ranged from 14.40±0.72 to 37.95±0.67. Results reveal that plant extracts studied possess anti-oxidant properties. Most potent extracts could be a lead to novel antioxidants and acetylcholinesterase inhibitors for the treatment of AD.
Acetylcholinesterase (AChE) inhibition and antioxidant activity are considered to be highly corre... more Acetylcholinesterase (AChE) inhibition and antioxidant activity are considered to be highly correlated with Alzheimer’s disease treatment. The present study was designed to investigate the antioxidant and acetylcholinesterase inhibitory activity of Desmodium gangeticum L. Properly identified powdered plant material was extracted successively using methanol as a solvent. Acetylcholinesterase inhibitory activity was measured with modified Ellman method at 405 nm and antioxidant activity measured based on 1, 1- diphenyl-2-picrylhydrazil (DPPH) free radical scavenging test at 517 nm. Percentage inhibition for AChE ranged from 28.78±0.12 to 40.83±0.05 whereas DPPH radical scavenging percentage ranged from 24.68±0.72 to 42.22±0.67.
Acetylcholinesterase (AChE) inhibition and antioxidant activity are considered to be highly corre... more Acetylcholinesterase (AChE) inhibition and antioxidant activity are considered to be highly correlated with Alzheimer’s disease treatment. The present study was designed to investigate the antioxidant and acetylcholinesterase inhibitory activity of Desmodium gangeticum L. Properly identified powdered plant material was extracted successively using methanol as a solvent. Acetylcholinesterase inhibitory activity was measured with modified Ellman method at 405 nm and antioxidant activity measured based on 1, 1- diphenyl-2-picrylhydrazil (DPPH) free radical scavenging test at 517 nm. Percentage inhibition for AChE ranged from 28.78±0.12 to 40.83±0.05 whereas DPPH radical scavenging percentage ranged from 24.68±0.72 to 42.22±0.67.
Acetylcholinesterase (AChE), the predominant cholinesterase in the brain, hydrolyzes ACh to choli... more Acetylcholinesterase (AChE), the predominant cholinesterase in the brain, hydrolyzes ACh to choline and acetate, thereby terminating the effect of this neurotransmitter at cholinergic synapses. Therefore, AChE is the target of cholinesterase inhibitors used for addressing the cholinergic deficit in Alzheimer's disease (AD) patients. Despite decades of research and advances in our understanding of its aetiology and pathogenesis, current pharmacotherapeutic options for AD are still very limited and represent an area of need that is currently unmet. The leading AD therapeutics involves AChE inhibitors, resulting in increased acetylcholine concentrations in the synaptic cleft and enhanced cholinergic transmission. Compounds showing an AChE inhibitory effect are also used for the treatment of senile dementia, myastenia gravis, Parkinson's disease and ataxia. Taking into account that the inhibition of AChE has been one of the most used strategies for treating AD and that existing drugs are effective only against mild to moderate type of disease while presenting considerable side effects, the search for new sources of effective and selective anti acetylcholinesterase agents with fewer side effects is imperative. Various plants and phytochemical substances have demonstrated AChE inhibitory activity and thus could be beneficial in the treatment of neurodegenerative disorders such as AD.
The methanolic extracts of Allium sativum were investigated for its antioxidant activity by using... more The methanolic extracts of Allium sativum were investigated for its antioxidant activity by using 1,1-diphenyl-2- picryl hydrazyl (DPPH) radical scavenging assay and by ferric reducing antioxidant power (FRAP) assay. The total
phenolic content was evaluated by Folin-Ciocalteu procedure. This study indicated that Allium sativum L. exhibited the
high antioxidant activity and phenolic contents andcan be used potentially as a readily accessible source of natural
antioxidant. Due to its natural origin and potent free-radical scavenging ability, Allium sativum L. could be used as a
potential preventive intervention for free radical-mediated diseases.
Keywords: Allium sativum L., Antioxidant activity, DPPH, FRAP, Phenolic content.
In the present work an attempt has been made to compare the in vitro antioxidant activities of cr... more In the present work an attempt has been made to compare the in vitro antioxidant activities of crude extracts O.sanctum using Water, Methanol and Ethanol as solvent by DPPH scavenging assay. Ethanolic extract gave maximum yield of crude extract as well as antioxidant activity. The analyzed plant extracts showed the scavenging of DPPH radicals in the range from 58.45±0.2 to 92.82±0.5. These values are comparable with Ascorbic acid as standard. These findings suggest that the rich phytochemical content of O.sanctum and its good antioxidant activity may be responsible for its popular and wide traditional use.
KEY WORDS: O. sanctum, DPPH, Antioxidant activity, ascorbic acid, Free radical.
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phenolic content was evaluated by Folin-Ciocalteu procedure. This study indicated that Allium sativum L. exhibited the
high antioxidant activity and phenolic contents andcan be used potentially as a readily accessible source of natural
antioxidant. Due to its natural origin and potent free-radical scavenging ability, Allium sativum L. could be used as a
potential preventive intervention for free radical-mediated diseases.
Keywords: Allium sativum L., Antioxidant activity, DPPH, FRAP, Phenolic content.
KEY WORDS: O. sanctum, DPPH, Antioxidant activity, ascorbic acid, Free radical.
phenolic content was evaluated by Folin-Ciocalteu procedure. This study indicated that Allium sativum L. exhibited the
high antioxidant activity and phenolic contents andcan be used potentially as a readily accessible source of natural
antioxidant. Due to its natural origin and potent free-radical scavenging ability, Allium sativum L. could be used as a
potential preventive intervention for free radical-mediated diseases.
Keywords: Allium sativum L., Antioxidant activity, DPPH, FRAP, Phenolic content.
KEY WORDS: O. sanctum, DPPH, Antioxidant activity, ascorbic acid, Free radical.