Free radicals contribute to more than one hundred disorders in humans. The synthetic antioxidants... more Free radicals contribute to more than one hundred disorders in humans. The synthetic antioxidants have been suspected to cause or prompt negative health effects leading to extensive research on naturally occurring antioxidants especially from plant sources. The present study describes the antioxidant efficacy of methanol extracts of three medicinal plants namely Drypetes roxburghii Wall, Coscinium fenestratum Colebr and Nardostachys jatamansi DC using three methods DPPH free radical scavenging assay, Hydroxyl radical scavenging assay and Fe+3 reducing assay. The dried plant materials were powdered, extracted using methanol in soxhlet apparatus and subjected to preliminary phytochemical analysis. The extracts concentrations namely 0.5 and 1.0mg/ml were screened for antioxidant activity by above three methods. The presence of various constituents namely alkaloids, flavonoids, tannins, steroids, glycosides were detected in methanol extracts. In DPPH free radical assay, potent scavengin...
Free radicals contribute to more than one hundred disorders in humans. In the present study, the ... more Free radicals contribute to more than one hundred disorders in humans. In the present study, the methanol extracts of three traditionally used medicinal plants Nardostachys jatamansi DC, Coscinium fenestratum Colebr and Drypetes roxburghii Wall were evaluated for antioxidant activity (DPPH free radical scavenging assay), total phenol (Folin Ciocalteu reagent method) and flavonoid content (Aluminium chloride colorimetric method). High phenol content was detected in D. roxburghii (213.33mg/g) followed by C. fenestratum (54.60mg/g) and N. jatamansi (53.33mg/g). Flavonoid content, expressed in terms of quercetin equivalent, was found to be high in case of N. jatamansi (31.25mg/g) which was followed by D. roxburghii (23.12mg/g) and C. fenestratum (20.00mg/g). Among the extracts, N. jatamansi exhibited high free radical scavenging activity (0.5mg- 86.70%; 1mg- 90.15%) followed by D. roxburghii (0.5mg- 73%; 1mg-88.19%) and C. fenestratum (0.5mg- 64.00%; 1mg- 83.01%). According to our study...
In vitro antioxidant activity of Putranjiva roxburghii Wall seed by DPPH radical scavenging activ... more In vitro antioxidant activity of Putranjiva roxburghii Wall seed by DPPH radical scavenging activity Putranjiva roxburghii Wall; Antioxidant activity; Soxhlet extraction; DPPH free radical scavenging assay; Ascorbic acid. KEYWORDS ABSTRACT Antioxidants help to deal with oxidative stress, caused by free radical damage. The present study highlights the antioxidant activity of methanol extract of P. roxburghii by its DPPH free radical scavenging potential. Various phytoconstituents namely alkaloids, flavonoids, saponins, glycosides, coumarins, quinones, tannins and steroids were detected. A marked radical scavenging activity was observed and found to be dose dependent. The activity of extract was found to be slightly lower as compared to the scavenging activity of ascorbic acid. Though in vitro antioxidant assay has been carried out, in vivo tests remains to be done.
In this study, we investigated the larvicidal activity of the methanolic extracts from traditiona... more In this study, we investigated the larvicidal activity of the methanolic extracts from traditionally used medicinal plants namely Putranjiva roxburghii Wall., Coscinium fenestratum Colebr, and Nardostachys jatamansi DC against larvae of Aedes aegypti. The larvae were taken in beakers were exposed to methanolic extracts of the plants at concentrations of 1, 2.5 and 5mg/ml for 24hours followed by counting of dead larvae and percentage mortality was calculated. The LC50 was also determined for each extract.All extracts showed larval mortality. Larval mortality was 100%with the use of 2.5 and 5%concentration of extract of each plant. The LC50 for N. jatamansi, P. roxburghii and C.fenestratum was found to be 0.83mg/ml, 0.90mg/ml and 1.25mg/ml respectively suggestingN. jatamansi is more potent followed by P. roxburghii and C. fenestratum. Qualitative analysis of the extracts revealed the presence of tannin, alkaloid, steroid and flavonoid in all the extracts. Saponin was not detected in N...
The present study was carried to investigate phytochemical profile, antimicrobial and wormicidal ... more The present study was carried to investigate phytochemical profile, antimicrobial and wormicidal activity of ethanol and methanol extracts of Nardostachys jatamansi DC and Coscinium fenestratum Colebr obtained by Soxhlet extraction process. Agar well diffusion method was employed to check the efficacy of extracts against bacteria and fungi. Wormicidal activity was assessed by determining paralysis and death time in earthworm model. The solvent extracts of the selected plants showed the presence of various phytochemical groups. A marked antibacterial activity was observed in case of C. fenestratum extracts followed by N. jatamansi. The extracts of N. jatamansi were found to inhibit test fungi to more extent than C. fenestratum. A dose dependent wormicidal activity was observed. Among extracts tested, N. jatamansi was found to be more efficient in terms of its activity as compared to C. fenestratum. the antimicrobial and wormicidal activity of extracts could be mainly attributed to th...
Preliminary phytochemical and antimicrobial activity ofCurcuma aromatica (rhizome) and Coscinium ... more Preliminary phytochemical and antimicrobial activity ofCurcuma aromatica (rhizome) and Coscinium fenestratum (stem) was investigated. The powdered plant materials were subjected to soxhlet extraction using ethanol as solvent. Phytochemical screening revealed the presence of flavonoids, saponins, tannins, glycosides, alkaloids and steroids in both the extracts. The ethanol extracts of the plant materials were subjected to antibacterial activity (Agar well diffusion method) andAntifungal activity (Poison food technique). The bacterial strains were seeded on media by Spread plate method and test fungi were point inoculated. Diameter of zone of inhibition was recorded (for bacteria) and colony diameter in poisoned plates (for fungi) was measured to assess the effect of extracts on test organisms. Both the extracts have shown marked antibacterial and antifungal activity against bacteria and fungi tested. Ethanol extract of Cosciniumfenestratum was found to inhibit bacteria to more extent...
Antioxidants help to deal with oxidative stress, caused by free radical damage. The present study... more Antioxidants help to deal with oxidative stress, caused by free radical damage. The present study highlights the antioxidant activity of methanol extract of P. roxburghii by its DPPH free radical scavenging potential. Various phytoconstituents namely alkaloids, flavonoids, saponins, glycosides, coumarins, quinones, tannins and steroids were detected. A marked radical scavenging activity was observed and found to be dose dependent. The activity of extract was found to be slightly lower as compared to the scavenging activity of ascorbic acid. Though in vitro antioxidant assay has been carried out, in vivo tests remains to be done.
The present study reports the efficacy of Mesua ferrea (Linn.) extract in mitigating radiation in... more The present study reports the efficacy of Mesua ferrea (Linn.) extract in mitigating radiation induced toxicity along with immunomodulatory property. Swiss albino mice, splenic lymphocytes and plasmid pBR322 DNA were used to evaluate the radioprotective effect by exposing them to 6Gy EBR or 4 Gy gamma radiation. In this study, Mesua ferrea methanolic (MfM) or aqueous extracts (MfA) significantly protected pBR322 DNA against radiation induced strand breaks. Both the extracts significantly offered protection against radiation-induced apoptosis as indicated by propidium iodide staining and DNA ladder assay. MfM or MfA significantly scavenged radiation derived free radicals indicating their anti-oxidant potential. MfM or MfA were orally administered to Swiss albino mice at 250 or200 mg/kg body weight respectively for 7 days. The study showed significant increase in the levels of glutathione, and activities of endogenous antioxidant enzymes superoxide dismutase & catalase. Administration of MfA or MfM to mice significantly reduced electron beam radiation (EBR; 6Gy) induced increase in MDA levels. Immunomodulatory efficacy of MfM and MfA was evaluated using concanavalin-A (Con-A) induced proliferation of CFSE labelled splenic lymphocytes. Both the extracts significantly reduced proliferation in a dose-dependent manner. Further, MfM or MfA treatment prevented EBR induced histopathological changes in jejunum, spleen, liver and kidney. This demonstrates that the plant Mesua ferrea has promising antioxidant, radioprotective and immunomodulatory activity which may be attributed to the active constituents present in it.
Free radicals contribute to more than one hundred disorders in humans. The synthetic antioxidants... more Free radicals contribute to more than one hundred disorders in humans. The synthetic antioxidants have been suspected to cause or prompt negative health effects leading to extensive research on naturally occurring antioxidants especially from plant sources. The present study describes the antioxidant efficacy of methanol extracts of three medicinal plants namely Drypetes roxburghii Wall, Coscinium fenestratum Colebr and Nardostachys jatamansi DC using three methods DPPH free radical scavenging assay, Hydroxyl radical scavenging assay and Fe+3 reducing assay. The dried plant materials were powdered, extracted using methanol in soxhlet apparatus and subjected to preliminary phytochemical analysis. The extracts concentrations namely 0.5 and 1.0mg/ml were screened for antioxidant activity by above three methods. The presence of various constituents namely alkaloids, flavonoids, tannins, steroids, glycosides were detected in methanol extracts. In DPPH free radical assay, potent scavengin...
Free radicals contribute to more than one hundred disorders in humans. In the present study, the ... more Free radicals contribute to more than one hundred disorders in humans. In the present study, the methanol extracts of three traditionally used medicinal plants Nardostachys jatamansi DC, Coscinium fenestratum Colebr and Drypetes roxburghii Wall were evaluated for antioxidant activity (DPPH free radical scavenging assay), total phenol (Folin Ciocalteu reagent method) and flavonoid content (Aluminium chloride colorimetric method). High phenol content was detected in D. roxburghii (213.33mg/g) followed by C. fenestratum (54.60mg/g) and N. jatamansi (53.33mg/g). Flavonoid content, expressed in terms of quercetin equivalent, was found to be high in case of N. jatamansi (31.25mg/g) which was followed by D. roxburghii (23.12mg/g) and C. fenestratum (20.00mg/g). Among the extracts, N. jatamansi exhibited high free radical scavenging activity (0.5mg- 86.70%; 1mg- 90.15%) followed by D. roxburghii (0.5mg- 73%; 1mg-88.19%) and C. fenestratum (0.5mg- 64.00%; 1mg- 83.01%). According to our study...
In vitro antioxidant activity of Putranjiva roxburghii Wall seed by DPPH radical scavenging activ... more In vitro antioxidant activity of Putranjiva roxburghii Wall seed by DPPH radical scavenging activity Putranjiva roxburghii Wall; Antioxidant activity; Soxhlet extraction; DPPH free radical scavenging assay; Ascorbic acid. KEYWORDS ABSTRACT Antioxidants help to deal with oxidative stress, caused by free radical damage. The present study highlights the antioxidant activity of methanol extract of P. roxburghii by its DPPH free radical scavenging potential. Various phytoconstituents namely alkaloids, flavonoids, saponins, glycosides, coumarins, quinones, tannins and steroids were detected. A marked radical scavenging activity was observed and found to be dose dependent. The activity of extract was found to be slightly lower as compared to the scavenging activity of ascorbic acid. Though in vitro antioxidant assay has been carried out, in vivo tests remains to be done.
In this study, we investigated the larvicidal activity of the methanolic extracts from traditiona... more In this study, we investigated the larvicidal activity of the methanolic extracts from traditionally used medicinal plants namely Putranjiva roxburghii Wall., Coscinium fenestratum Colebr, and Nardostachys jatamansi DC against larvae of Aedes aegypti. The larvae were taken in beakers were exposed to methanolic extracts of the plants at concentrations of 1, 2.5 and 5mg/ml for 24hours followed by counting of dead larvae and percentage mortality was calculated. The LC50 was also determined for each extract.All extracts showed larval mortality. Larval mortality was 100%with the use of 2.5 and 5%concentration of extract of each plant. The LC50 for N. jatamansi, P. roxburghii and C.fenestratum was found to be 0.83mg/ml, 0.90mg/ml and 1.25mg/ml respectively suggestingN. jatamansi is more potent followed by P. roxburghii and C. fenestratum. Qualitative analysis of the extracts revealed the presence of tannin, alkaloid, steroid and flavonoid in all the extracts. Saponin was not detected in N...
The present study was carried to investigate phytochemical profile, antimicrobial and wormicidal ... more The present study was carried to investigate phytochemical profile, antimicrobial and wormicidal activity of ethanol and methanol extracts of Nardostachys jatamansi DC and Coscinium fenestratum Colebr obtained by Soxhlet extraction process. Agar well diffusion method was employed to check the efficacy of extracts against bacteria and fungi. Wormicidal activity was assessed by determining paralysis and death time in earthworm model. The solvent extracts of the selected plants showed the presence of various phytochemical groups. A marked antibacterial activity was observed in case of C. fenestratum extracts followed by N. jatamansi. The extracts of N. jatamansi were found to inhibit test fungi to more extent than C. fenestratum. A dose dependent wormicidal activity was observed. Among extracts tested, N. jatamansi was found to be more efficient in terms of its activity as compared to C. fenestratum. the antimicrobial and wormicidal activity of extracts could be mainly attributed to th...
Preliminary phytochemical and antimicrobial activity ofCurcuma aromatica (rhizome) and Coscinium ... more Preliminary phytochemical and antimicrobial activity ofCurcuma aromatica (rhizome) and Coscinium fenestratum (stem) was investigated. The powdered plant materials were subjected to soxhlet extraction using ethanol as solvent. Phytochemical screening revealed the presence of flavonoids, saponins, tannins, glycosides, alkaloids and steroids in both the extracts. The ethanol extracts of the plant materials were subjected to antibacterial activity (Agar well diffusion method) andAntifungal activity (Poison food technique). The bacterial strains were seeded on media by Spread plate method and test fungi were point inoculated. Diameter of zone of inhibition was recorded (for bacteria) and colony diameter in poisoned plates (for fungi) was measured to assess the effect of extracts on test organisms. Both the extracts have shown marked antibacterial and antifungal activity against bacteria and fungi tested. Ethanol extract of Cosciniumfenestratum was found to inhibit bacteria to more extent...
Antioxidants help to deal with oxidative stress, caused by free radical damage. The present study... more Antioxidants help to deal with oxidative stress, caused by free radical damage. The present study highlights the antioxidant activity of methanol extract of P. roxburghii by its DPPH free radical scavenging potential. Various phytoconstituents namely alkaloids, flavonoids, saponins, glycosides, coumarins, quinones, tannins and steroids were detected. A marked radical scavenging activity was observed and found to be dose dependent. The activity of extract was found to be slightly lower as compared to the scavenging activity of ascorbic acid. Though in vitro antioxidant assay has been carried out, in vivo tests remains to be done.
The present study reports the efficacy of Mesua ferrea (Linn.) extract in mitigating radiation in... more The present study reports the efficacy of Mesua ferrea (Linn.) extract in mitigating radiation induced toxicity along with immunomodulatory property. Swiss albino mice, splenic lymphocytes and plasmid pBR322 DNA were used to evaluate the radioprotective effect by exposing them to 6Gy EBR or 4 Gy gamma radiation. In this study, Mesua ferrea methanolic (MfM) or aqueous extracts (MfA) significantly protected pBR322 DNA against radiation induced strand breaks. Both the extracts significantly offered protection against radiation-induced apoptosis as indicated by propidium iodide staining and DNA ladder assay. MfM or MfA significantly scavenged radiation derived free radicals indicating their anti-oxidant potential. MfM or MfA were orally administered to Swiss albino mice at 250 or200 mg/kg body weight respectively for 7 days. The study showed significant increase in the levels of glutathione, and activities of endogenous antioxidant enzymes superoxide dismutase & catalase. Administration of MfA or MfM to mice significantly reduced electron beam radiation (EBR; 6Gy) induced increase in MDA levels. Immunomodulatory efficacy of MfM and MfA was evaluated using concanavalin-A (Con-A) induced proliferation of CFSE labelled splenic lymphocytes. Both the extracts significantly reduced proliferation in a dose-dependent manner. Further, MfM or MfA treatment prevented EBR induced histopathological changes in jejunum, spleen, liver and kidney. This demonstrates that the plant Mesua ferrea has promising antioxidant, radioprotective and immunomodulatory activity which may be attributed to the active constituents present in it.
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Phytochemical Investigation and Hepatoprotective A by Syed Murthuza
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