Pulmonary hypertension (PH), associated with imbalance in vasoactive mediators and massive remodeling of pulmonary vasculature, represents a serious health complication. Despite the progress in treatment, PH patients typically have poor... more
Pulmonary hypertension (PH), associated with imbalance in vasoactive mediators and massive remodeling of pulmonary vasculature, represents a serious health complication. Despite the progress in treatment, PH patients typically have poor prognoses with severely affected quality of life. Asymmetric dimethyl arginine (ADMA), endogenous inhibitor of endothelial nitric oxide synthase (eNOS), also represents one of the critical regulators of pulmonary vascular functions. The present study describes a novel mechanism of ADMA-induced dysfunction in human pulmonary endothelial and smooth muscle cells. The effect of ADMA was compared with well-established model of hypoxia-induced pulmonary vascular dysfunction. It was discovered for the first time that ADMA induced the activation of signal transducer and activator of transcription 3 (STAT3) and stabilization of hypoxia inducible factor 1α (HIF-1α) in both types of cells, associated with drastic alternations in normal cellular functions (e.g., nitric oxide production, cell proliferation/Ca(2+) concentration, production of pro-inflammatory mediators, and expression of eNOS, DDAH1, and ICAM-1). Additionally, ADMA significantly enhanced the hypoxia-mediated increase in the signaling cascades. In summary, increased ADMA may lead to manifestation of PH phenotype in human endothelial and smooth muscle cells via the STAT3/HIF-1α cascade. Therefore this signaling pathway represents the potential pathway for future clinical interventions in PH.
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Security incidents are becoming more serious and more common not only in computer networks, but also in automation networks. Automation devices are still more and more based on computers and they have the same weak points like standard... more
Security incidents are becoming more serious and more common not only in computer networks, but also in automation networks. Automation devices are still more and more based on computers and they have the same weak points like standard computers. Actual trends in automation networks are among others wide automation networks covering several manufacture divisions or remote controlling of automation networks
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This paper is focused on the Internet Graphical Simulation Tool for computer analysis of electronic circuits and describes all main features and advantages of using this tool for computer modeling and simulations of electronic circuits.... more
This paper is focused on the Internet Graphical Simulation Tool for computer analysis of electronic circuits and describes all main features and advantages of using this tool for computer modeling and simulations of electronic circuits. Internet Graphical Simulation Tool (IGST) provides easy way for modeling and simulation of electronic circuits without necessity of purchasing expensive software license or installing some special simulation software tools. IGST can be used anywhere access to the Internet is available. IGST is composed from three main parts which provide basic PSpice analyzing functionality for any electronic circuit. The first part of the IGST is a GUI for interactive drawing and editing of electronic circuit's schema. The second part is a simulation core from freely available older version of the PSpice simulation software and the last part of the IGST is an Internet graphical environment for displaying simulation results. Based on these three parts the IGST creates complex tool for easy analysis of electronic circuits through the Internet.