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Gastroenterology. Author manuscript; available in PMC 2022 March 01.
Published in final edited form as:
Gastroenterology. 2021 March ; 160(4): 1240–1255.e3. doi:10.1053/j.gastro.2020.11.008.
A novel role of SLC26A3 in maintenance of intestinal epithelial
barrier integrity
Anoop Kumar1,2,#,$, Shubha Priyamvada2,#, Yong Ge3,#, Dulari Jayawardena2, Megha
Singhal2, Arivarasu N. Anbazhagan2, Ishita Chatterjee2, Aneal Dayal2, Mitul Patel2, Kimia
Zadeh, Seema Saksena1,2, Waddah A. Alrefai1,2, Ravinder K Gill2, Mojgan Zadeh3, Ni Zhao3,
Mansour Mohamadzadeh3, Pradeep K. Dudeja1,2,*,$
Author Manuscript
1Jesse
Brown VA Medical Center, University of Illinois at Chicago, Chicago, IL.
2Division
of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at
Chicago, Chicago, IL.
3Department
of Infectious Diseases and Immunology and Division of Gastroenterology,
Hepatology and Nutrition, Department of Medicine, University of Florida, Gainesville, FL.
Abstract
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Background: The Down Regulated in Adenoma (DRA) encoded by SLC26A3, a key intestinal
chloride anion exchanger, has recently been identified as a novel susceptibility gene for
inflammatory bowel disease (IBD). However, the mechanisms underlying the increased
susceptibility to inflammation induced by the loss of DRA remain elusive.
Aims: Compromised barrier is a key event in IBD pathogenesis, the current studies were
undertaken to elucidate the impact of DRA deficiency on epithelial barrier integrity and to define
underlying mechanisms.
Methods: WT and DRA KO mice and crypt-derived colonoids were used as models for intestinal
epithelial response. Paracellular permeability was measured using FITC (Fluorescein
Isothiocyanate)-dextran flux. Immunoblotting, immunofluorescence, immunohistochemistry and
*
Author Manuscript
Author to whom correspondence should be addressed: Pradeep K. Dudeja, Ph.D., Senior Research Career Scientist, Jesse Brown
VA Medical Center, Professor of Physiology in Medicine, University of Illinois at Chicago, Medical Research Service (600/151), Jesse
Brown VA Medical Center, 820 South Damen Avenue Chicago, IL 60612, pkdudeja@uic.edu, Tel.: (312)-569-7434 Fax.
(312)-569-6487.
#These authors equally contributed to this work.
$Co-senior authors
Author contributions: Conceptualization: AK, SP, YG, DJ, MS, ANA, SK, IC, KZ, SS, WAA, RKG, MZ, NZ, MM, PKD; Data
Curation: AK, SP, YG, NZ, DJ; Formal Analysis: AK, SP, YG, NZ, DJ, MS, ANA, PKD; Funding Acquisition: AK, PKD, WAA, SS,
RKG,MM; Investigation: AK, SP, YG,NZ, DJ, MS, ANA, MZ, AD, MP; Methodology: AK, SP, YG, NZ, MZ, DJ, MS, ANA, AD,
MP; Project Administration: AK, SP, PKD; Resources: AK, MM, PKD, WAA, SS, RKG; Software: AK, YG, NZ, DJ, ANA, MZ,
MM, PKD; Supervision: AK, PKD, WAA, RKG, SS, MM, Validation: AK, YG, NZ, AD, WAA, PKD; Visualization: AK, YG, NZ,
DJ, MZ,KZ, PKD; Writing (original draft): AK, YG, MM, SP; Writing (editing and revising): AK, SP, YG, DJ, MS, ANA, IC, MP,
SS, WAA, RKG, MM, PKD
Conflict of interest: none
We demonstrate that DRA (key chloride transporter) plays an important role in maintaining gut barrier function as loss of the DRA
gene promotes leaky gut implicated in development of IBD.
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Kumar et al.
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RNP-IP (Ribonucleoprotein Immunoprecipitation) assays were performed. Gut microbiome
analysis was conducted to demonstrate the role of DRA deficiency on gut microbial communities.
Results: DRA KO mice exhibited an increased colonic paracellular permeability with
significantly decreased levels of tight junction/adherens junction (TJ/AJ) proteins, including ZO-1,
occludin and E-cadherin. A similar expression pattern of occludin and E-cadherin was observed in
colonoids derived from DRA KO mice and shRNA-mediated DRA knock-down in Caco-2 cells.
Microbial analysis showed gut dysbiosis in DRA KO mice. However, cohousing studies showed
that dysbiosis only played a partial role in maintaining TJ protein expression. Furthermore, our
results showed increased binding of RNA-binding protein CUGBP1 with occludin and E-cadherin
genes in DRA KO mouse colon, suggesting post-transcriptional mechanisms play a key role in gut
barrier dysfunction.
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Conclusion: Our studies demonstrate a novel role of DRA in maintaining the intestinal epithelial
barrier function and potential implications of its dysregulation in IBD pathogenesis.
Graphical Abstract
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Keywords
Inflammatory bowel disease; Intestinal chloride transporter; Down regulated in adenoma; Gut
microbiota
Introduction:
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Inflammatory Bowel Diseases (IBD), including Crohn’s disease (CD) and ulcerative colitis
(UC), are chronic inflammatory intestinal disorders. The etiology of IBD is a multifactorial
complex interaction that is believed to manifest from a combination of genetic,
environmental, immunological and gut microbial triggers1, 2. Specifically, one of the key
events in the pathogenesis of IBD consists of impaired epithelial barrier function that may in
turn lead to increased dissemination of pathogens and their gene products (e.g., toxins)
locally and peripherally in the host3.
Previous studies have shown that changes in expression and/or distribution of tight junction
(TJ) proteins lead to disrupted barrier function, observed in active CD and UC patients3.
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Further, a number of studies have shown that TJ dysregulation may be in response to
cytokines or induced gut inflammation that activate distinct transcriptional and posttranscriptional mechanisms4–6. However, there is still a significant gap in our knowledge in
understanding the molecular mechanisms involved in regulating TJ barrier function during
normal homeostasis and disease manifestation. Of particular note, the intestinal ion
transporters, NHE3 and DRA, have recently emerged as the key regulators of epithelial
homeostasis7, 8. Therefore, their dysregulation may critically perturb the epithelial regulation
contributing to the development and exacerbation of IBD.
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DRA is the main chloride transporter involved in the intestinal vectorial NaCl absorption1.
Inhibition of DRA expression and function has been implicated in diarrheal diseases in
response to pathogen infection and gut inflammation1. Furthermore, mutations in the DRA
gene result in congenital chloride diarrhea associated with high fecal chloride content.
Additionally, DRA expression is drastically reduced in all models of colitis as well as in IBD
patients1, 9, 10. DRA knockout mice (DRA KO mice) phenotypically display a higher IBD
risk, including lack of the adherent inner mucus layer, altered proliferative homeostasis of
the colonic crypts and increased susceptibility to experimental colitis11, 12.
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A recent genome wide association study (GWAS) identified SLC26A3 (DRA) as a novel
IBD susceptibility gene in Japanese cohorts13. Similarly, a recent study performed in
Chinese patients found the association of SLC26A3 polymorphism leading to decreased
DRA expression with higher risk for ulcerative colitis 14. Additionally, another recent study
showed that 7 damaging variants, including SLC26A3, were located within the IBDassociated loci of Icelandic UC patients. SLC26A3 was noted to have a CADD score above
20, indicating that DRA was amongst the top 1% of deleterious variants for IBD in the
human genome15. These data strongly suggest that DRA may not only be involved in
chloride absorption but may also play an integral role in maintaining gut epithelial
homeostasis. However, whether dysfunctional DRA plays an important role in the
development of gut inflammation and the associated mechanisms remain unclear.
This study provides strong evidence for the novel role of DRA in maintaining intestinal
epithelial integrity and identifies underlying mechanisms. By utilizing DRA KO mice, we
demonstrated that the loss of DRA resulted in markedly increased colonic paracellular
permeability, decreased TJ and AJ proteins and gut dysbiosis. Moreover, our studies also
demonstrated that loss of DRA disrupted the TJ and AJ proteins via post-transcriptional
mechanisms with no alterations in the mRNA levels. Thus, optimal DRA expression in the
colonic epithelium may be integral for maintaining gut epithelial integrity and its loss in
colonic epithelium could potentially contribute to the pathogenesis of IBD.
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Materials and Methods:
Anti-occludin, claudin 1, 2, 3, ZO-1 and E-cadherin antibodies were obtained from
Invitrogen (Carlsbad, CA) and Cell Signaling (Danvers, MA). The C-terminal amino acid
sequence INTNGGLRNRVYEPVETKF of DRA was utilized to generate a DRA antibody at
the Research Resources Center at the University of Illinois at Chicago (UIC)16. FITC-
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dextran was purchased from Sigma-Aldrich (St. Louis, MO). RNA extraction and RT-PCR
kits were procured from Qiagen (Valencia, CA) and Agilent (La Jolla, CA), respectively.
In vivo studies:
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Prior approval for performing in vivo studies in C57BL/6 and DRA KO mice was obtained
from the Institutional Animal Care Committee of Jesse Brown Veterans Affairs Medical
Center and UIC. DRA KO mice were maintained on an electrolyte solution in drinking
water to prevent dehydration17. For the first 3–4 weeks after birth and before weaning, WT
mice were cage mates with DRA knockout mice and thus received the same electrolyte
solution. Before sacrifice, WT mice were again maintained on the same electrolyte solution
for 2 weeks in order to have an appropriate control for the experiments. The intestinal tissues
were removed and the mucosa was harvested for isolating RNA and protein extraction.
Additionally, the distal colons were snap frozen for immunofluorescence studies. For the
cohousing studies, wild type and DRA KO mice were co-housed together for 4 weeks on
electrolyte solution and the fecal matter were collected pre- and post-cohousing.
Dextran sulfate sodium (DSS) treatment:
C57BL/6J mice (male, 8 weeks of age, Jackson laboratory) were divided into two groups to
either administer 3% DSS (wt/vol) (MP Biomedical, Solon, OH) in drinking water for 7
days or drinking water alone (control). Mice were euthanized and distal colonic mucosal
scrapings were harvested for protein extraction.
Mouse colonoids:
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Mouse colonic crypts were isolated and colonoids were cultured as previously described 16.
Briefly, each colon was dissected, rinsed with ice-cold PBS (penicillin, 100 IU/ml/
streptomycin), and divided into 1 cm sections. Tissues were incubated in PBS with 2mM
EDTA at 4°C for 30 minutes on a shaker. Subsequently, tissues were treated with 54.9 mM
D-sorbitol and 43.4 mM sucrose in PBS, followed by vortexing the tissues thoroughly for 1–
2 minutes before filtration through a 100-μm sterile cell strainer. Filtrates were centrifuged at
150 rpm at 4°C for 10 minutes to collect the crypts, which were placed in growth factor
reduced phenol-free Matrigel (BD Biosciences). Next, 60-μl droplets of Matrigel/crypt mix
were placed in the center of each well of a 12-well plate in a 37°C incubator for Matrigel
polymerization. Following gel polymerization, 700 μl/well of mouse organoid media
(Stemcell Technologies, Vancouver, Canada) was added to the plates which were then
incubated at 37°C to promote differentiation into crypt-derived colonoids that were used for
experiments. The medium was changed every 2–3 days. Each experiment was repeated at
least 3 times during the study.
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Measuring intestinal paracellular permeability:
Mouse intestines were opened along the mesenteric border and mounted into an Easy Mount
Ussing chamber system (Physiologic Instruments, San Diego, CA). Tissues were subjected
to 5 ml Ringer solution (in mmol/l: 0.4 KH2PO4, 2.4 K2HPO4, 115 NaCl, 25 NaHCO3, 1.2
CaCl2, 1.2 MgCl2, 10 mannitol) on each side in a water-jacketed system maintained at
37°C. After an equilibration period of 10 min, solutions were replaced with fresh Ringer and
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4 kDa FITC-dextran (Sigma) was added to the luminal side to a final concentration of 2.2
mg/mL. Intestinal permeability was determined by comparing mucosal-to-serosal flux of
FITC dextran over 2 hours 18.
RNA extraction and gene expression:
RNeasy Mini Kits (Qiagen) were used for RNA extraction from mouse distal mucosal
scrapings. Equivalent amounts of RNA from each sample were reverse-transcribed and
amplified in a 1-step reaction using Brilliant III Ultra-fast SYBR Green QRT-PCR master
mix kit (Agilent) using Mx 3000 (Agilent). The gene-specific primers for mouse DRA,
occludin, ZO-1, claudins, cytokines and GAPDH have been described previously19 and are
also mentioned in Supplementary Table 1.
Western blotting:
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RIPA lysis buffer (Cell Signaling, Danvers, MA) was utilized for obtaining protein lysates
from the mucosa. Protein lysates were separated on 10% SDS-PAGE gels, transferred onto
nitrocellulose membranes, probed with anti-TJ/AJ antibodies, followed by visualization with
enhanced chemiluminescence detection reagent. Quantification of band intensity was
determined using ImageJ software (NIH, Bethesda, MD).
Immunofluorescence staining of mouse colonic tissues:
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Colonic tissues from WT and DRA KO mice were snap-frozen in OCT for immunostaining.
Tissue sections were fixed with 4% paraformaldehyde in PBS for 15 minutes, permeabilized
with 0.3% NP-40 for 5 minutes, followed by blocking with 5% normal goat serum (NGS)
for 2 hr. Antibodies for the TJ/AJ proteins were incubated at 1:100 dilution in PBS with 1%
NGS. After 4 washes, Alexa Fluor 594 and 488 conjugated goat anti-rabbit IgG (Invitrogen)
were used for 60 min. The sections were mounted using Slowfade Gold antifade with DAPI
reagent (Invitrogen), followed by imaging with a BX Fluorescent microscope (Olympus)
equipped with 100X oil immersion objective.
16S rRNA gene sequencing and data analysis:
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Total DNA was isolated from fecal and cecal samples using ZymoBIOMICS DNA Miniprep
Kit (Zymo Research). The V4-V5 regions of 16S rRNA gene were amplified using barcoded
dual-index primers 19, 2021. PCR amplicons were purified, quantified and pooled libraries
were sequenced on an Illumina Miseq instrument using a Miseq Reagent v3 kit (Illumina).
The 2 × 300 bp reads were processed using QIIME 2 (v2020.2.0). Briefly, paired-reads were
merged, quality trimmed and clustered into operational taxonomic units (OTUs) at 97%
sequence identity. Taxonomy was assigned using the Greengenes database. OTU tables were
rarefied to 45,000 sequences and the rarefied OTU tables were used to calculate alpha
diversity (Shannon index) and beta diversity (weighted Unifrac distance) to determine
relative abundances of taxa at multiple hierarchical levels. Linear discriminant analysis
effect size (LEfSe) was performed to identify bacterial species that significantly contributed
to microbiota difference by cohousing. Differentially significant taxa at each level were
identified by unpaired t-test or Mann–Whitney U test.
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Statistical Analysis:
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The data presented are mean ± SEM of 3–6 independent experiments. Unpaired Student’s ttest was utilized for statistical analysis. P ≤.05 was considered statistically significant.
Results:
Loss of DRA increases paracellular permeability in mouse colonic epithelium:
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DRA KO mice are known to exhibit a significant decrease in apical Cl−/HCO3− exchange
activity translating to markedly higher chloride levels and water content in the stool11.
Similarly, DRA KO mice demonstrated severe diarrhea, distended intestines and abdomen
(Figures 1A and 1B). Thus, these symptoms, in addition to genotyping, were utilized in
ascertaining the DRA KO phenotype. Previous studies demonstrated that DRA KO mice
exhibit decreased resistance to mucosal damage induced by DSS12. To further examine
whether the intestinal permeability is altered in DRA KO mice when compared to agematched wild type littermates, Ussing chambers were used to measure mucosal-to-serosal
FITC-dextran flux, as a surrogate for paracellular permeability in the proximal and distal
colons of wild type (WT) and DRA KO mice. Here, the colons for DRA KO mice exhibited
significantly higher paracellular permeability compared to those of WT mice in proximal
(Figure 1C) and distal colonic regions (Figure 1D). However, no significant changes in
paracellular permeability were observed in the ileal regions (Supplementary Fig 1A). These
results provide novel evidence for the perturbation of epithelial barrier function under basal
conditions, which may increase the susceptibility of these mice to experimental DSS
induced colitis.
Loss of DRA decreases tight junction (TJ) protein expression in mouse colonic tissue:
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Occludin and ZO-1 are the major transmembrane tight junction proteins that regulate the
paracellular permeability to specific ions and large molecules3. The effect of DRA
deficiency on the expression of the key TJ proteins was analyzed. There was a significant
decrease in expression of occludin and ZO-1 and an increase in the expression of the pore
forming protein claudin-2 in the distal colon of DRA KO mice (Figure 1E–H). Furthermore,
immunostaining also demonstrated similar changes in occludin and ZO-1 expression in the
distal colon of DRA KO mice, suggesting an increase in paracellular permeability (Figure 1I
and 1J). In contrast, claudin-1 and 3 protein expression did not show any significant changes
(Supplementary Figures 1B and C), indicating that the changes were specific to only certain
TJ proteins: occludin, ZO-1 and claudin-2.
DRA KO mice exhibit decreased colonic adherens junction proteins:
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In addition to TJ proteins, adherens junctions (AJ), also play an important role in
maintaining the host epithelial integrity22. Similar to TJ proteins, there was a significant
decrease in expression of the key AJ proteins, E-cadherin and β-catenin (Figure 2A and 2B).
Additionally, immunostaining analysis further confirmed decreases in E-cadherin expression
(Figure 2C) in the distal colon of DRA KO mice.
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Gut microbiota analysis in DRA KO mice:
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The gut microbiome is critical for maintenance of the intestinal epithelial barrier
integrity2324. To investigate whether the observed reduction of intestinal permeability in
DRA KO mice is related to the perturbation of gut microbiota, the bacterial communities
from fecal and cecal contents derived from WT and DRA KO mice were analyzed by 16S
rRNA sequencing. Bacterial diversity, as indicated by the Shannon index (measure of OTU
richness and evenness), was significantly decreased in both fecal and cecal samples of DRA
KO mice compared to WT mice (Figure 3A). Weighted Unifrac principal coordinate analysis
(PCoA) showed a distinct difference in both cecal and fecal microbiota derived from DRA
KO mice compared to WT mice (Figure 3B). In particular, the major phyla Actinobacteria
was diminished in DRA KO mice compared to WT mice in fecal samples (Figure 3C).
Although phylum Bacteroidetes was not significantly different between the groups,
substantial differences were observed at the bacterial family level (Figures 3C and 3D).
Compared to WT mice, DRA KO mice demonstrated decreased levels of Bacteroidales
family S24–7 and Rikenellaceae, and significantly increased levels of Bacteroidaceae and
Porphyromonadaceae in fecal and cecal samples (Figure 3D). Accordingly, Bacteroides
ovatus and the genus Parabacteroides exhibited significantly higher levels in DRA KO mice
compared to WT mice (Figure 3E). Within Firmicutes, Erysipelotrichaceae family members
were dominant in DRA KO mice but not WT mice (Figure 3D). This was largely accounted
for at the genus level by the greatly increased level of Allobaculum in the fecal and cecal
samples of DRA KO mice (Figure 3E). Notably, DRA KO mice demonstrated significantly
less butyrate-producing taxa compared to WT mice. This includes Butyricicoccus
pullicaecorum, Coprococcus, Ruminococcus, Odoribacter, Clostridium, Anaerostipes and
Oscillospira (Figure 3E and Supplemental Figure 3). Combined, these data suggest that loss
of DRA significantly impacts the composition of gut microbiota in mice.
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Effect of DRA deficiency on TJ/AJ proteins in mouse colonoids and Caco-2 cells:
Next, we examined TJ/AJ protein expression in colonoids generated from the DRA KO
mice, as well as in Caco-2 cells where DRA was silenced by shRNA. Representative blots
for colonoids (Figure 4A) and the associated densitometric analyses (Figures 4B and C)
exhibited a decrease in occludin and E-cadherin expression in the colonoids generated from
DRA KO mice. Additionally, shRNA-mediated-DRA-silenced Caco-2 cells also showed a
decrease in occludin expression by immunoblotting (Figures 4D–F).
DRA KO mice exhibited a moderate increase in some inflammatory cytokines while mRNA
expression of TJ/AJ proteins remained unchanged:
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Interestingly, DRA KO mice showed a moderate increase in the pro-inflammatory cytokines
CXCL1 and IL-1β (Supplementary Figures 2A and 2B). There were no significant changes
observed in TNFα and IFNγ transcripts in the distal colon of DRA KO mice
(Supplementary Figures 2C and 2D). With regards to TJ/AJ expression, it is noteworthy that
the decreased TJ/AJ protein expression occurred only at the protein level, without any
significant alterations in the transcripts of TJ/AJ (Supplementary Figures 2E, 2F, 2G, and
2H).
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Role of RNA binding proteins (RBPs) in TJ/AJ protein expression in colon of DRA KO
mice:
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Since there was no change in TJ/AJ protein transcripts in DRA KO mice, we explored the
regulation of TJ/AJ protein expression by potential post-transcriptional mechanisms. A
previous report demonstrated that RNA binding proteins HuR and CUGBP1 play a key role
in post-transcriptional regulation of TJ/AJ proteins25. Our data showed that CUGBP1
expression was significantly upregulated in the distal colon of DRA KO mice (Figure 4G).
Interestingly, the protein level of intact HuR was significantly decreased while cleaved HuR
(HuR CP-1) was significantly increased in DRA KO mice colon (Figure 4H). This finding is
important as the cleaved product (~24kDa) has been earlier shown to change the binding
ability of HuR to occludin and E-cadherin mRNA 3’ UTRs26. Further, we tested whether
increased expression of CUGBP1 leads to increased binding to the occludin and E-cadherin
3’UTRs leading to downregulation of their expression. Interestingly, our RNP-IP assay
results showed significant enrichment of the binding of CUGBP1 to occludin and E-cadherin
3’UTR (Figures 4I and 4J). Taken together, these data suggest that induction of CUGBP1
and cleavage of HuR play significant roles in the mechanisms underlying disruption of
barrier function in DRA KO mice.
Enhanced CUGBP1 and Cleaved HuR protein expression in DSS induced colitis:
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We and others previously demonstrated that DRA9 and tight junction proteins27 were
decreased in the distal colon of DSS-treated mice. Consistent with these reports, we
observed a similar decrease in DRA (Figure 5A) and occludin expression (Figure 5B) in the
distal colon of DSS treated mice. Interestingly, similar to DRA KO mice, DSS-induced
colitis in mice also exhibited a marked increase in the levels of CUGBP1 (Figure 5C) and
the cleaved-HuR protein (HuR CP-1). Further, there was a decrease in the level of intact
HuR (Figure 5D), indicating that changes in the expression of these RNA binding proteins,
as well as, the cleavage of HuR may have contributed to the downregulation of tight junction
protein expression in DSS-induced colitis.
Effects of cohousing on DRA KO and WT mice:
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Because mice are coprophagic, the potential for transfer of gut microbiota through the fecaloral route is high. Thus, we used a cohousing strategy to determine whether exposure of
DRA KO mice demonstrating a distinct microbial dysbiosis could impact the expression of
TJ/AJ proteins involved in the barrier integrity in WT mice or vice versa. Thus, 4 weeks,
post cohousing, a significant decrease in Shannon index was observed in the fecal samples
of WT mice, but not in DRA KO mice, compared to fecal samples collected from the same
mice before cohousing (Figure 6A). The weighted PCoA plot showed separate community
clusters of the unrelated DRA KO and WT mice, with the cohoused mice forming mostly
non-overlapping clusters between them (Figure 6B). This is consistent with reduced
weighted Unifrac distances between cohoused DRA KO and WT mice, indicating a higher
similarity than the comparison of their pre-cohoused counterparts (Figure 6C). This suggests
a normalization of bacterial community structure by cohousing. Furthermore, cohousing led
to a significant increase in Actinobacteria and a reduction in Bacteroidetes in both DRA KO
and WT mice (Figure 6D). Next, we investigated differences in the bacterial families and
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genera between cohoused DRA KO and WT mice. Here, Parabacteroides
(Porphyromonadaceae) was more abundant in cohoused DRA KO mice compared to WT
mice (Figure 6E and Supplementary Figure 3), which may suggest the limited capability of
this bacterial genus to transmit horizontally. Although Akkermansia muciniphila
(Verrucomicrobiaceae) and Blautia producta (Lachnospiraceae) were clearly higher in DRA
KO mice than the WT mice (Figure 6E and Supplementary Figure 3), significant numbers of
these bacteria were also found in WT mice during cohousing, as demonstrated by linear
discriminant analysis (LDA) for effect size (LEfSe) (Figure 6F). Notably, Allobaculum
(Erysipelotrichaceae) may potentially be acquired by WT mice from their cohoused partners
(Figures 6E and 6F), while Lactobacillus (Lactobacillaceae), dominantly populated in the
WT mice (Figure 6E), was the most distinguishing taxa enriched in cohoused DRA KO mice
(Figure 6F). Cohoused WT mice, compared to their pre-cohoused controls, also had
significantly more unclassified Enterococcaceae in their fecal samples, which was, by
contrast, reduced in cohoused DRA KO mice (Figure 6F). Additionally, cohousing favored
the propagation of some taxa in both DRA KO and WT mice, including Staphylococcus
sciuri, Corynebacterium stationis and Jeotgalicoccus psychrophilus (Figure 6F). Taken
together, these data suggest that cohousing of DRA KO and WT mice may provide
opportunities for some taxa mixing and microbial community shifts toward their cohoused
partners in the fecal samples.
Effect of cohousing on TJ/AJ protein expression in DRA KO mice:
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The effect of cohousing on the expression of key TJ/AJ proteins was analyzed to elucidate
the role of gut microbiome dysbiosis in DRA KO mice. Interestingly, our results
demonstrated a slightly less, but still significant, decrease in the TJ protein expression of
occludin and ZO-1 (Figure 7A–D) in distal colon of DRA KO mice. However, although the
changes in the protein expression of E-cadherin also showed a decrease after cohousing, this
decrease did not reach statistical significance (Figure 7A–D). Immunostaining also
demonstrated similar changes in occludin, ZO-1 and E-cadherin expression in the distal
colon of DRA KO mice after cohousing (Figure 7E). In addition, our results from co-housed
mice also showed similar changes in RNA binding proteins CUGBP-1 and HuR expression
in the DRA KO mice compared to wild type mice (Figure 7F and 7G) as seen in noncohoused mice (Figure 4G and 4H). These data indicate that microbial dysbiosis only partly
contributes to the decreases in TJ/AJ protein expression observed in DRA KO mice.
Discussion:
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Impaired intestinal epithelial barrier function is one of the key risk factors in the
pathogenesis of IBD 28. However, the mechanisms underlying the dysregulation of intestinal
TJ and AJ barriers under pathophysiological conditions are still evolving. Here, we present
strong evidence highlighting the critical role of the chloride transporter DRA in maintaining
the intestinal barrier integrity in a mouse model deficient in DRA.
DRA is a major intestinal epithelial apical membrane Cl−/HCO3− exchanger that is pivotal
in maintaining fluid and electrolyte absorption in the intestine. Mutations in the DRA gene
in humans lead to congenital chloride diarrhea (CLD), which requires life-long supplemental
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electrolyte therapy29. Similar to the phenotype observed in CLD patients, DRA KO mice
exhibit a diarrheal phenotype11, 12, a lack of an adherent inner mucus layer and are more
prone to experimental colitis12. Furthermore, DRA expression is significantly reduced in
animal models of infectious colitis30 and inflammation-associated diarrhea1, 9, 10.
Importantly, several genome wide association studies (GWAS) in Japanese, Chinese and
Icelandic cohorts suggest that DRA deficiency or mutations in DRA gene are associated
with higher risk for the development of ulcerative colitis1, 1314.
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Our current studies demonstrated that the DRA KO mouse colon exhibited significantly
higher paracellular permeability compared to WT mice. Our data further showed a
significant decrease in the expression of the TJ proteins occludin and ZO-1 and a marked
increase in claudin-2 expression in DRA KO mice. In addition to alterations in TJ proteins,
the AJ proteins, including E-cadherin and β-catenin were also significantly reduced in DRA
KO mice. Interestingly, the maximum decrease in TJ/AJ protein expression was observed in
the distal colon, which was the same region where the highest increase in paracellular
permeability occurred when compared to the proximal colon and ileum in DRA KO mice.
This is consistent with the finding that DRA expression is highest in the distal colon
compared to other intestinal regions. The expression pattern of DRA along the length of
intestine appears to parallel the barrier defect observed and, thus, may partly explain the
maximum effects on TJ proteins and barrier function observed in the distal colon of DRA
KO mice.
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To further elucidate the direct role of DRA in gut barrier integrity, studies were also carried
out with colonoids derived from DRA KO mice. Similar to our data in DRA KO mice,
occludin and E-cadherin expression was significantly downregulated in DRA KO colonoids.
These data indicate that DRA downregulation can also directly perturb the epithelial barrier
under basal conditions, which may increase susceptibility of DRA KO mice to experimental
colitis and associated diarrhea in the absence of microbial dysbiosis. In this regard, data
from caco-2 cells with shRNA-mediated knock-down of DRA also showed downregulation
of occludin levels, further indicating the direct involvement of DRA in maintaining TJ
integrity.
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To delineate the mechanism(s) underlying the changes observed in TJ/AJ proteins in DRA
KO mice, we further examined the transcript levels of occludin, ZO-1, claudin-2 and Ecadherin. Interestingly, the gene expression of these proteins remained unchanged in distal
colon of DRA KO mice, suggesting the involvement of post-transcriptional mechanisms.
Previous studies showed that the RNA binding proteins CUGBP1 (CUG-binding protein 1)
and HuR conjointly regulate the translation of occludin and E-cadherin and play a key role
in TJ and AJ maintenance in IECs25. However, while HuR acts as a positive regulator,
CUGBP1 acts as a negative regulator of TJ/AJ proteins by competing with HuR for binding
to the 3’UTR of occludin and E-cadherin22. In our current studies, the decrease of occludin
and E-cadherin protein levels in response to loss of DRA was accompanied by an increased
expression in CUGBP1 and a significant decrease in intact HuR with a concomitant increase
in HuR-Cleaved Protein1 (HuR CP-1). Further, increased direct binding of CUGBP-1 to
occludin and E- cadherin 3’UTR as assessed by RNA-IP assay clearly showed that post
transcriptional modulation of TJ protein levels by RBPs may be one of the key mechanisms
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underlying barrier disruption in response to loss of DRA. We also demonstrated that DSScolitis mice (which exhibit significant downregulation of DRA and barrier impairment), also
showed a significantly increased level of CUGBP1 and a decreased level of intact HuR.
However, DSS-induced colitis models are also known to exhibit histological evidence of
mucosal damage and higher expression of proinflammatory cytokines, such as IL-1β, which
has been shown to reduce the TJ/AJ protein expression at the mRNA level. DRA KO mice,
however, did not show any histologic evidence of mucosal damage and showed significantly
smaller increases in IL-1β, and did not exhibit any changes in the mRNA levels of occludin,
ZO-1, claudin-2 and E-cadherin. Taken together, this suggests that the changes observed in
gut permeability and TJ proteins in DRA KO mice were predominantly due to the loss of
DRA and alterations in RBPs and not mediated by inflammatory mediators.
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The gut microbiome plays an important role in maintaining intestinal permeability2324. In
depth microbial analysis (down to the genus and species level) demonstrated that DRA
deficiency markedly altered the gut microbial community. Specifically, DRA KO mice
showed significantly reduced microbial diversity in the fecal and cecal contents. The phylum
Actinobacteria was significantly decreased in the feces of DRA KO mice. A very recent
study has also briefly shown microbial changes at the phylum level in DRA KO mice similar
to the one reported here while being fed a special diet31. Interestingly, previous studies have
reported that a decreased relative abundance of Actinobacteria is associated with a stressinduced increase in gut permeability32 Also, patients with irritable bowel syndrome (IBS)
show decreased abundance of Actinobacteria in stool samples33. The genus Allobaculum,
which has been shown to be associated with intestinal inflammation and a leaky gut in
mouse and rat models, was significantly enriched in the stool samples derived from DRA
KO mice in this study34. Additionally, Parabacteroides, which has been implicated in the
susceptibility of NHE3 KO mice to colitis, and Bacteroides ovatus, which has been
implicated in the pathogenesis of IBD, were significantly enriched in fecal and cecal
samples of DRA KO mice in this study35. Importantly, DRA KO mice exhibited
significantly reduced levels of numerous butyrate-producing Clostridia bacteria, including
Butyricicoccus pullicaecorum, Coprococcus, Ruminococcus, Clostridium, Anaerostipes and
Oscillospira. Notably, butyrate promotes intestinal barrier function through the regulation of
TJ protein assembly and expression36, 37. Butyrate also supports the oxygenation of
intestinal epithelial cells, stabilizes the transcription factor HIF-1 and augments epithelial
barrier function38. Thus, it is conceivable that increased intestinal permeability of DRA KO
mice at least in part be due to the decreased level of butyrate-producing microbial
community in the gut. It is generally accepted that cohousing normalizes the microbiota
between mouse strains due to coprophagy. We consistently observed an increasingly similar
composition of gut microbiota between DRA KO mice and WT mice after cohousing
compared to their pre-cohoused microbiota, with bacterial taxa such as Allobaculum and
Lactobacillus significantly enriched in the cohoused WT partners. Furthermore, changes in
microbial composition were accompanied by the expansion of some proinflammatory
bacteria such as Facklamia and Staphylococcus, which are normally suppressed by
colonization resistance. Additionally, Akkermensia muciniphila, an important mucindegrading intestinal bacterium, was evidently increased in WT mice through cohousing
effects of DRA KO mice, which may underlie the thin mucus layer observed in DRA KO
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mice12. Notably, after cohousing, DRA KO mice still showed a significant, decrease in the
ZO-1 and occludin protein expression and significant increase in CUGBP1 and cleaved HuR
levels when compared to mice that were not cohoused. However, the maximum effect of cohousing was observed on E-cadherin expression. Taken together, our data showed that
optimal DRA expression plays a critical role in shaping the gut microbiota, and epithelial
barrier defects in DRA KO mice may be only partly attributed to changes in the gut
microbiome. Translating this mechanism to human disease, DRA deficiency, thus, may be a
critical contributor to dysbiosis observed in patients with IBD.
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In summary, our studies demonstrate that DRA plays a critical role in the maintenance of
colonic barrier function, expression and regulation of TJ/AJ proteins, and healthy microbial
community and its deficiency may lead to barrier impairment, microbial dysbiosis and
development of IBD.Thus, it appears that DRA upregulation may have a dual beneficial
impact in IBD by not only counteracting diarrhea, (the most debilitating symptom of IBD,
through enhancing electrolyte absorption) but also correcting barrier disruption associated
with IBD.
Supplementary Material
Refer to Web version on PubMed Central for supplementary material.
Acknowledgments:
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Supported by Department of Veterans Affairs CDA2 Award # BX004719 (A. Kumar) VA Merit Award # BX002011
(P. K. Dudeja), VA Merit Award # BX000152 (W. A. Alrefai), VA Merit Award BX002867 (S. Saksena), and VA
Senior Research Career Scientist Award # 1IK6BX005242-01 (P. K. Dudeja), Research Career Scientist Award #
1IK6BX005243-01 (W.A. Alrefai), and the NIDDK grants DK54016, DK92441 (P.K. Dudeja), DK109709 (W. A.
Alrefai), DK98170 (R.K. Gill), DK109560 (M. Mohamadzadeh).
We acknowledge the help of Dr. Theodor Griggs and Mr. Nathan Calzadilla in proofreading this manuscript.
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Background:
DRA (intestinal chloride transporter) has been suggested to be a novel susceptibilty gene
in IBD pathogenesis. However, the mechanisms underlying loss of DRA in increasing
susceptibility to inflammation remain elusive.
New Findings:
Our data demonstrate that loss of DRA results in increased intestinal paracellular
permeability, microbial dysbiosis and decrease in tight/adherens junction proteins via
posttranscriptional mechanism.
Limitation:
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Further studies are needed to establish if the similar mechanism is involved in patients
with IBD.
Impact:
DRA upregulation may serve as a novel therapeutic approach with dual impact in IBD
via beneficial effects on gut epithelial integrity as well as in counteracting IBD associated
diarrhea.
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Figure 1: Loss of DRA results in increased paracellular permeability and decreased TJ protein
expression in mouse colon:
DRA KO mice exhibit severe diarrhea, a distended abdomen and enlarged cecum (Figures
A and B). DRA KO mice showed increased paracellular permeability in proximal colon
(PC) and distal colon (DC) (Figures C and D). Intestinal permeability was determined by
comparing mucosal-to-serosal flux of 4 kDa-FITC dextran (FD4) fluorescence over 120
minutes (n=3–4). Effects of DRA knockdown on protein levels of TJ components in wild
type and DRA KO mice distal colon by immunoblotting are shown (Figure E) (n=3–6).
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GAPDH was used as the internal control. Densitometric analyses of band intensities are
shown (Figures F, G, H). Values are means ± SEM. Data was analyzed by unpaired t-test
(*p <0.05 vs. wild type; **p <0.01 vs. wild type). Immunofluorescence staining of distal
colon sections of wild type and DRA KO mice for occludin (green), villin (red), and DAPI
(blue) (Figure I) and ZO-1 (red), villin (green), and DAPI (blue) (Figure J). Scale bar: 20
μm.
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Figure 2: Loss of DRA results in decreased AJ protein expression in mouse colon:
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Distal colonic mucosal lysates of wild type and DRA KO mice were subjected to SDSPAGE and probed with E-cadherin and β-catenin antibodies. (Figures A and B):
Densitometric analysis was performed of relative band intensities with GAPDH as internal
control. Values are means ± SEM and representative blots of 3–6 independent experiments
are shown. Data was analyzed by unpaired t-test (*p <0.05 vs. wild type; **p <0.01 vs. wild
type). (C) Immunofluorescence staining of distal colonic sections of wild type and DRA KO
mice for E-cadherin (red), villin (green), and DAPI (blue) is shown. Scale bar: 20 μm.
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Figure 3. Loss of DRA alters gut microbial composition.
(Figure A) Shannon index measuring bacterial diversity by 16S rRNA sequencing in the
feces and cecal contents of DRA KO and WT mice (n=3). (Figure B) Weighted Unifrac
principal coordinate analysis (PCoA) plot separating fecal and cecal microbiota composition
between DRA KO and WT mice. (Figure C) Top phylum abundance in the feces and cecal
contents. (Figure D) Top family abundance of fecal and cecal microbes. Asterisks (*)
indicate statistical significance between the groups of mice. (Figure E) Significantly
different taxa between DRA KO and WT mice in fecal and cecal samples in red. The dashed
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lines represent thresholds (log2 fold change > 1, FDR P <0.05). *p <0.05, **p <0.01, ***p
<0.001.
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Figure 4: Loss of DRA results in reduced TJ/AJ protein expression in colonoids and Caco-2 cells
and increased CUGBP1 expression, HuR protein cleavage, and binding of CUBGP1 to 3’ UTR of
occludin and E-cadherin in DRA KO mice:
Protein expression of E-cadherin and occludin in colonoids from wild type and DRA KO
mice by western blotting (Figure A). Densitometric analyses of band intensities (GAPDH
was used as the internal control) are shown (Figures B and C). DRA and occludin
immunoblotting in lentiviral shRNA mediated knockdown of DRA in Caco-2 cells are
shown (Figure D). Densitometric analyses of band intensities (GAPDH was used as the
internal control) are shown (Figures E and F). Values (for figures 4B,4C,4E,4F) are means
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± SEM from blots from 3–4 independent experiments. Data was analyzed by unpaired t-test
(**p <0.01 vs. wild type). Western blot and densitometric analysis of CUGBP1 and HuR
protein levels in distal colonic mucosal lysates of wild type or DRA KO mice (Figures G
and H). Results represent means ± SEM of 3–4 mice (**p <0.01 compared to wild type,
###p <0.001, cleaved compared with intact HuR). CUGBP1 binding (over IgG) to 3’UTR of
occludin and E-cadherin as measured by RNP-IP Assay (Figures I and J). Values are means
± SEM of blots of 4–5 independent experiments. Data was analyzed by unpaired t-test (*p
<0.05 compared to wild type).
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Figures 5: DSS-colitis results in decreased protein levels of DRA, occludin, and increased protein
levels of CUGBP1 and cleaved-HuR:
Representative western blots and densitometric analysis of DRA and occludin protein levels
in distal colonic mucosal tissue lysates of DSS or control mice (Figures A and B). Results
represent means ± SEM of 4 mice. (*p <0.05 and **p <0.01 compared to controls).
Representative western blots and densitometric analysis of CUGBP1 and HuR protein
(intact and cleaved HuR) levels in distal colonic mucosal tissue lysates of wild type or DRA
KO mice (Figures C and D). Results represent means ± SEM of 3 mice. Data was analyzed
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by unpaired t-test (*p <0.05 CUGBP1 in DSS mice compared to control mice. #p <0.05,
cleaved HuR/intact HuR in DSS mice compared to control mice).
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Figure 6. Cohousing contributes to partial exchange of fecal microbiota in DRA KO and WT
mice.
Shannon index of fecal microbiota in post-cohoused DRA KO and WT mice compared to
their pre-cohoused counterparts (n=5) (Figure A). Weighted Unifrac PCoA plot showing
community variation in indicated mouse groups (Figure B). Weighted Unifrac distances
between cohoused mice and their pre-cohoused controls showing the reduced similarity
between DRA KO and WT mice after cohousing (Figure C). Averaged relative abundances
of top phyla (Figure D) and families (Figure E) in pre- and post-cohoused DRA KO and
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WT mice. LEfSe plots showing differentiating taxa (species level) between post-cohoused
mice and their pre-cohoused controls (Figure F). *p <0.05, **p <0.01, pre-cohoused WT vs.
post-cohoused WT; #p <0.05, ##P <0.01, pre-cohoused KO vs. post-cohoused KO.
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Figure 7: TJ/AJ protein and RBPs expression in cohoused wild type and DRA KO mice:
Protein expression of ZO-1, occludin and E-cadherin in distal colon of wild type and DRA
KO co-housed mice (Figure A). Densitometric analyses (GAPDH was used as the internal
control) of band intensities are shown (Figures B, C and D). Values are means ± SEM of
blots from 4 wild type and 5 KO co-housed mice. Data was analyzed by unpaired t-test (*p
<0.05 vs wild type, **p <0.01 vs. wild type). Immunostaining for ZO-1, occludin, and Ecadherin is shown (Figure E). Scale bar: 20 μm. Protein expression of CUGBP-1 and HuR
in distal colon of wild type and DRA KO co-housed mice by western analysis and
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densitometric analyses (GAPDH-internal control) of band intensities is shown (Figures F
and G). Values are means ± SEM of blots from 4 wild type and 5 KO mice. Data was
analyzed by unpaired t-test (*p <0.05 vs wild type, ## p <0.01 vs. wild type).
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