639
Journal of Physiology (1993), 469, pp. 639-652
With 7 figures
Printed in Great Britain
BLOCK OF CURRENT THROUGH T-TYPE CALCIUM CHANNELS
BY TRIVALENT METAL CATIONS AND NICKEL IN
NEURAL RAT AND HUMAN CELLS
By BORIS MLINAR AND JOHN J. ENYEART
From the Department of Pharmacology and the Neuroscience Program,
The Ohio State University College of Medicine, Columbus, OH 43210-1239, USA
(Received 17 November 1992)
SUMMARY
1. The effects of the trivalent cations yttrium (Y3 ), lanthanum (La3"), cerium
(Ce3+), neodymium (Nd3+), gadolinium (Gd3+), holmium (Ho3"), erbium (Er3"),
ytterbium (Yb3+ ) and the divalent cation nickel (Ni2") on the T-type voltage gated
calcium channel (VGCC) were characterized by the whole-cell patch clamp technique
using rat and human thyroid C cell lines.
2. All the metal cations (M3+) studied, blocked current through T-type VGCC (IT)
in a concentration-dependent manner. Smaller trivalents were the best T-channel
antagonists and potency varied inversely with ionic radii for the larger M3+ ions.
Estimation of half-maximal blocking concentrations (IC50s) for IT carried by 10 mM
Ca2" resulted in the following potency sequence: Ho3" (IC50 = 0 107 /4M) t Y3
(0 117) Yb3+ (0 124) > Er3+ (0 153) > Gd3+ (0 267) > Nd3+ (0 429) > Ce3+ (0 728) >
La3+ (1-015) ~> Ni2+ (5 65).
3. Tail current measurements and conditioning protocols were used to study the
influence of membrane voltage on the potency of these antagonists. Block of IT by
Ni2+, Y3+ La3+ and the lanthanides was voltage independent in the range from -200
to + 80 mV. In addition, the antagonists did not affect macroscopic inactivation
and deactivation of T-type VGCC.
4. Increasing the extracellular Ca2+ concentration reduced the potency of IT
block by Ho3+, indicative of competitive antagonism between this blocker and the
permeant ion for a binding site.
5. The results suggest that the mechanism of metal cation block of T-type VGCC
is occlusion of the channel pore by the antagonist binding to a Ca2+/M3+ binding
site, located out of the membrane electric field.
6. Block of T-type VGCC by Y3+, lanthanides and La3+ differ from the inhibition
of high voltage-activated VGCC block in several respects: smaller cations are more
potent IT antagonists; block is voltage independent and the antagonists do not
permeate T-type channels. These differences suggest corresponding structural
dissimilarities in the permeation pathways of low and high voltage-activated Ca2+
channels.
MS 1914
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640
B. MLINAR AND J. ENYEART
INTRODUCTION
Trivalent cations including Y3, La3" and the lanthanides (elements 58-71 in the
periodic table) share biologically important chemical properties with the divalent
calcium (Ca2") cation (Nieboer, 1975; Williams, 1982; Evans, 1990). Their similarity,
especially in ionic radii, co-ordination chemistry and preference for the oxygen
donor groups, have provided the basis for wide use of Y3+, La3" and the lanthanides
in studying the function of Ca2" in biological systems (Nieboer, 1975; reviewed in
Evans, 1990). Because of their strong interaction with Ca2' binding sites on
membrane proteins (dos Remedios, 1981), they have been useful in studying
voltage-gated calcium channels, where they potently inhibit Ca21 currents.
Previous studies of Ca21 channel block by trivalent metal cations (M3+) have
focused primarily on high voltage-activated voltage-gated calcium channels (VGCC)
(see Hille, 1992 for VGCC nomenclature and a review). Several of the more extensive
studies have provided valuable insights into high voltage-activated VGCC structure
and function (Nachsen, 1984; Lansman, Hess & Tsien, 1986; Lansman 1990). In
contrast, block of low voltage-activated T-type VGCC by trivalent metal cations
has not been systematically studied. Similarly, the blocking properties of Ni2", a
widely used T-type channel antagonist, have not been described. Several reports
have appeared describing the potency of T-type VGCC block by La3" (Narahashi,
Tsunoo & Yoshii, 1987; Akaike, Kostyuk & Osipchuk, 1989; Akaike, Kanaide,
Kuga, Nakamura, Sadoshima & Tomoike 1989), Gd3+ (Biagi & Enyeart, 1990) and
Y3+ (Biagi & Enyeart, 1991).
Using neural crest-derived rat and human thyroid C cell lines, we found that
Y3+ and smaller lanthanides are the most potent inorganic antagonists of T-type
VGCC. The properties of IT block by M3 and Ni2" indicate that structural
differences exist between T-type and high voltage-activated Ca2" channels.
METHODS
Materials
Tissue culture media, horse serum, and fetal calf serum were obtained from Gibco (Grand
Island, NY, USA). Culture dishes were purchased from Corning (Corning, NY, USA). YCl3 and
lanthanide chlorides (at least 99.9 % purity) were obtained from Aldrich Chemical Co. Ltd
(Milwaukee, WI, USA). All other chemicals were purchased from Sigma Chemical Co. Ltd (St
Louis, MO, USA).
Cell culture
The rat medullary thyroid carcinoma 6-23 (clone 6) cell line (rat C cells) was purchased from
the American Type Culture Collection and grown in 35 mm dishes on poly-D-lysine-coated
coverslips in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10 % horse serum
at 37 °C in a humidified atmosphere of 95 % air and 5 % CO2. The human medullary thyroid
carcinoma TT cell line (human C cells), kindly provided by Andree de Bustros (John Hopkins
University), was grown on coverslips as described above in DMEM supplemented with 10 % fetal
bovine serum.
Solutions and bath perfusion
The standard electrode filling solution was (mM): 120 CsCl, 1 CaCl2, 2 MgCl2, 111,2-bis(Oaminophenoxy)ethane-N,N,N7,N7-tetraacetic acid (BAPTA), 10 Hepes and 1 MgATP, with pH
titrated to 7-25 + 0 05 using CsOH. The standard external solution consisted of (mM):
117 tetraethylammonium chloride (TEACl), 5 CsCl, 10 CaCl2, 2 MgCl2, 5 Hepes, 10 glucose, with
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METAL CATION BLOCK OF T-TYPE CALCIUM CHANNELS
641
pH adjusted to 7-3 using TEAOH. In experiments with different concentrations of extracellular
CaCl2 isosmolarity was maintained by adjusting the concentration of TEACI. All solutions were
filtered through 0-22 ,um cellulose acetate filters.
Handling of Y, La3+ and the lanthanides is greatly restricted by their specific chemical
properties (reviewed by Evans, 1990). To avoid formation and precipitation of insoluble M(OH)3
and M2(CO3)3, as well as formation of radiocolloids and loss of M3+ ions to the container surface,
millimolar aqueous stock solutions of MC13 were prepared daily in polyethylene microtubes. Stock
solutions were diluted to final concentration directly in the bath perfusion system immediately
before use. The perfusion system consisted of polyethylene and polypropylene containers and
tubing, since Y3+, La3+ and the lanthanides strongly bind to negatively charged groups on glass
surfaces. The recording chamber (volume 1 ml) was continuously perfused by gravity at a rate
of 5-6 ml/min. Bath solution exchange was done by a manually controlled six-way rotary valve,
flushing at least 10 ml of perfusate.
In spite of the relatively constant activity coefficients of trivalent lanthanide chlorides in
aqueous solution and precautions taken in experimental procedures, free concentration of
unhydrolysed M(H2O).31 ionic species in the extracellular solution cannot be determined with
certainty. Partial hydrolysis of M3+ results in rapid formation of significant quantities of
relatively soluble M(OH)2+, M(OH)2' and other species (Biedermann & Ciavatta, 1961; Evans,
1990), the final concentrations of which are largely unknown. It is likely that some of the
hydrolysis products contributed to the observed effects in this study.
Recording conditions and electronics
Rat and human C cells were used in patch clamp experiments 1-4 days after plating. Coverslips with cells were transferred from 35 mm culture dishes to the recording chamber. Spherical
cells 10-40 ,sm in diameter and without processes were selected for recording. Patch electrodes
with resistances of 1-2 MQl were fabricated from 10O10 glass (Corning) and R-6 glass (Garner
Glass Co., Claremont, CA, USA) using a Brown-Flaming Model P-80 microelectrode puller
(Sutter Instruments, Novato, CA, USA). Access resistance during recording was 2-5 Mfl. Wholecell currents were recorded at room temperature (22-24 °C) following the procedure of Hamill,
Marty, Neher, Sakmann & Sigworth (1981), using a List EPC-7 (List-Medical, Darmstadt,
Germany) or an Axopatch ID (Axon Instruments, Inc., Burlingame, CA, USA) patch clamp
amplifier. Pulse generation and data acquisition were done using an IBM-AT computer and
pCLAMP software with an Axolab interface (Axon Instruments, Inc.). Currents were filtered
using an 8-pole low-pass Bessel filter (Frequency Devices, Haverhill, MA, USA) with cut-off
frequency (-3 dB) set at 1-22 kHz and digitized at 1-3-100 kHz. Linear leak and capacity
currents were subtracted from current records using summed scaled hyperpolarizing steps of 1/2
to 1/6 pulse amplitude.
Data were analysed and plotted using pCLAMP (CLAMPAN and CLAMPFIT) and InPlot 4
(GraphPAD Software, San Diego, CA, USA) programs and an IBM-compatible PC. All fits to
single exponential functions were done from the point of maximal decay to the end of current
records using the pCLAMP least-squares regression subroutine. Inhibition curves are InPlot 4
least-square regression fits, where current in control saline is normalized to 1 and assuming
complete block of current with sufficient concentration of antagonists. All quantitative results are
given as the means + S.E.M. or, in the case of least-square fits, as the estimate + S.E.E. (standard
error of the estimate).
Identification of IT in rat C cells
The majority of experiments were performed on rat C cells, which express prominent T-type
Ca2l current (IT). Occasionally, experiments as noted in the text were performed using human C
cells which express only T-type VGCC, but have less prominent IT (Biagi, Mlinar & Enyeart, 1992).
In rat C cells, IT can be easily distinguished from other Ca2+ current components by its voltagedependent and kinetic properties (Biagi & Enyeart, 1991). When voltage clamp steps are applied
at test potentials between -30 and -15 mV, the low threshold IT is selectively activated, and
appears as a transient component of Ca21 current which inactivates completely. A distinctive
feature of T-type Ca2+ channels is their slow rate of closing, which upon repolarization after a
short test depolarization is observed as a slowly decaying 'tail current'. For our studies, IT was
isolated as an inactivating component of Ca2+ current present at test potentials more negative
than -15 mV, or as a tail current measured 1P5 to 3 ms after repolarization to -80 mV.
,
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642
B. MLINAR AND J. ENYEART
RESULTS
Concentration-dependent block of T-type VGCC by Y3+, La3" and lanthanides
Y3+, La3+ and each of six lanthanides studied were found to block IT at submicromolar concentrations. Examples of concentration-dependent block by four of these
agents are shown in Fig. 1. Block by any of the M3+ ionswas only partially
reversible upon switching to control perfusate, even after 10 min of washing.
However, inclusion of 50-100 /M EGTA (which potently chelates Y3+, La3+ and
lanthanides) in the control solution resulted in the rapid and usually complete
reversal of the inhibition (Fig. 1E).
To quantitate the relative potency of the M3+ ions as IT antagonists, inhibition
curves were constructed for each of the eight elements, after measuring relative
block at a variety of concentrations (Fig. 2A). IC50s varied over a tenfold range
from 107 + 5 nM (Ho3+) to 1015 + 1 nm (La3+). Inhibition curves for all of the
M3+ had Hill coefficients close to -1 (-0-98 to -1 33), a characteristic of 1:1
ligand:receptor binding.
The relationship between ionic radii of the eight M3+ ions and their respective
potency as antagonists of T-type Ca2` channels is shown in Fig. 2B. The most
potent block (smallest IC50) was produced by the smaller lanthanides and Y3+. For
elements with radii above 0 102 nm (Gd3+ to La3+ ), potency varied inversely with
ionic radius.
To determine whether M3+ ions compete with Ca2` for specific binding sites on Ttype VGCC, inhibition curves were constructed for Ho3+ block of IT carried by 2, 10
and 50 mm Ca2+. As expected for a competitive antagonist, increasing the external
Ca2+ concentration reduced the potency of Ho3+ as evidenced by a nearly parallel
shift to the right in the inhibition curve (Fig. 3).
Voltage (in)dependence of block of IT by Y3+, La 3+ and lanthanides
The potency of ion channel block by charged antagonists often depends on the
conducting state of the channel and therefore on the transmembrane potential.
Voltage-dependent block of L-type VGCC by di- and trivalent metal cations is well
documented (Lansman et al. 1986; Lansman, 1990). Experiments were designed to
determine whether block of T-type VGCC is voltage dependent. Transition from
open to non-conducting states may be faster in the presence of an antagonist which
preferentially blocks open channels. To determine whether such a change in
kinetics occurs during the block of IT with M3+, we compared rates of macroscopic
inactivation and deactivation with and without M3+ in the solution.
All eight M3+ ions tested failed to change the macroscopic inactivation rate, which
occurred along a single exponential time course. For example, in control saline, the
inactivation time constant of IT was 18-5 + 0-2 ms (n = 6) at a test potential of -20 mV. After addition of 20, 100 and 500 nm Er3+, the corresponding time
constants were 18-2 + 0-2 ms (n = 6), 18-9 + 0 3 ms (n = 5) and 18-4 + 0-6 ms (n = 5).
Figure 4A shows scaled traces illustrating the absence of any effect of Gd3+ on
macroscopic IT inactivation.
The effects of M3+ on deactivation kinetics was studied by comparing tail currents
upon repolarization to -80 mV, after an activating test pulse. Deactivation, like
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a single exponential
which could
be fitted
occurredfrom
byJ aPhysiol
process
inactivation,
METAL CATION BLOCK OF T-TYPE CALCIUM CHANNELS
A
643
B
Y3+
03
3 ms
50 ms
C
GdP+
D
0. 5m
0.
3 ms
50 Ms
E
3m
&-~
1 *0
t
0*O-5 Gd3+
.0
0./
0-1 Gd3+ *|..
.0
0
0
0
0
0
a
I-,
_F-
0-5
2*5 Gd3+
*.
00
00
50 EGTA
*
0
a
0
0
L
o
2
4
8
6
10
Time (min)
Fig. 1. Concentration-dependent block of IT by M3+ rat C cells. A-D, superimposed
current records illustrating block of IT by four different M3+ concentrations. The averaged
traces in descending order of amplitude, represent steady-state current in: A, control
saline (0 Y3+, 10 mm Ca"+), 50, 250 and 500 nM Y3; B, control, 50, 200 and 1000 nM Yb3+;
C, control, 0 1, 0 5 and 2-5 /SM Gd3+; D, control, 0-1, 0-5, 2-5j/M Nd3+. Currents were
activated by depolarizing steps of either 300 (A and C) or 10-5 (B and D) ms duration,
applied at 0-1 Hz from a holding potential of-80 mV. Test potentials were: A, -24 mV; B,
C and D, -20 mV. Tail currents were recorded at -80 mV. E, time course of IT block by
Gd3+ (concentrations in FM) followed by reversal upon superfusion of saline containing
50 FM FGTA. Data from the same cell as shown in C.
m
time course. All M3+ ions were tested in at least four cells; each failed to affect
deactivation kinetics (data not shown). The tail currents shown in Fig. 4B
deactivated with time constants (-d) of 214
Y3+) and
(50
(control), 2412
In
the
same cell, Td in the presence of 500 nM y3+ was 2-09 ms
2-05 ms (250 nM Y3+).
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ms
ms
nM
B. MLINAR AND J. ENYEART
644
A
1-0r
o Yb3+
y3+
o Ho3+
* Er3+
A
0)
c
c
E
A
a)
0-5
C
0
F
Gd3+
. Nd3+
* Ce3+
. La3+
._o0
cJ
0
-9
-8
-7
log [M3+] (log M)
-6
-5
B
1.00
0
r
0-75 r
I
0
0*50
lo
0-25
A
3A
0
0-00
0-100
0-105
0-110
Ionic radius (nm)
0-115
Fig. 2. Potency of IT block by M3+ and relationship to ionic radius. A, inhibition curves for
eight different M3+ ions. Data obtained in experiments as described in legend of Fig. 1.
Data points are normalized mean values obtained in 5-10 (usually 6) separate
measurements after steady-state block was reached. Inhibition curves are best fits of data
to an equation of the form:
Y= 1/1 +
(1C50/X)X
where Y is the fraction of control current remaining after addition of the antagonist and X
is the log of antagonist concentration. IC50 and Hill slope (h) have been estimated from the
fits. IC50s and Hill slopes for the M3` ions are: 107 + 5 nM, -1P27 + 0-08 for Ho3+;
117 + 8 nM, -P105 + 0.09, Y3+; 125 + 1 nM, -P107 + 0-01, Yb3+; 153 + 9 nm, -P118 + 0-08,
Er3+; 267 + 7 nm, -P133 + 0-04, Gd3+; 429 + 81 nm, -0-98 + 0-21, Nd3+; 728 + 43 nM,
-0-98 + 0-06, Ce3+; 1015 + 1 nM, -P125 + 0-001, La3+. B, potency as a function of ionic
radius. IC50s of eight M3+ ions are plotted against corresponding cationic radius (Shannon,
1976), assuming a co-ordination number of 8. Although 8 is the most likely co-ordination
number for Y3+, lanthanides and La3+ under most biological conditions (Moeller, Martin,
Thompson, Ferrus, Feistel & Randal, 1965; Nieboer, 1975), the presence of ionic species
with other co-ordination numbers is probable. Symbols representing IC50s are for the same
M3+ as those in A.
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645
METAL CATIONBLOCK OF T-TYPE CALCIUM CHANNELS
(data not shown). Using the human C cells, it was possible to study the effects of
M3+ on deactivation kinetics over a wide range of potentials (Fig. 4 C). Neither
Y3 (n = 2) nor La3" (n = 2) affected rd at repolarization potentials ranging from
-200 to -60 mV.
C 50mMCa2+
B 10mMCa2+
A 2mMCa21
Ho3+
0-1 PM Ho3l
0.1 PM HO3+
Control
0
a ms
3ms
Control
Control
3ms
1*0
E
0
0
-9
-8
-7
-6
-5
log [Ho3+] (log M)
Fig. 3. The effect of Ca2+ concentration on potency of IT block by HO3+. A-C, the inhibition
of IT tail currents by 01 /UM Ho3` was measured at three different external Ca2+
concentrations. Tail current records before and after superfusing 01 /SM Ho3` in the
presence of 2, 10 and 50 mm external Ca2+ as indicated. Voltage protocols were similar to
those described in the legend of Fig. 1. Test potentials in A (-25 mV) and C (0 mV) were
adjusted to account for shifts in the current-voltage relationship, produced by changing the
external Ca2+ concentrations. D, inhibition curves for Ho3+ block of IT. Data points (n = 5-7)
have been calculated as described in the legend of Fig. 1. IC50s for Ho3+ block and Hill slopes
of corresponding curves are: 24-6 + 2-4 nM, -1-23 + 0-15, in 2 mm Ca2+ (U); 107 + 5 nM,
-1-27 + 0-08, in 10 mM Ca2+ (A); 716 ± 45 nm, 04J97 ± 0-06, in 50 mM Ca2+ (@).
To explore the possibility of slowly developing voltage-dependent block, we
tested whether changing the conditioning and/or holding potential affected the
potency of the M3` antagonists. Conditioning pulses of 20 s duration to potentials
ranging from -95 to -45 mV were ineffective in changing the potency of 100 nM
Ho3+ (n= 1) or 1 /SM La3+ (n = 1; Fig. 5 A) in rat C cells. Changes in holding potential
did not affect the block by 750 nM Y3+ (n = 1) and 750 nm La3+ (n = 1) in human C
cells (data not shown).
In addition to the lack of voltage dependence, no evidence of frequency-dependent
block was observed. Increasing the stimulation frequency to values between 0-008
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B. MLINAR AND J. ENYEART
646
and 10 Hz did not change the potency of La3" in two rat C cells (Fig. 5 B), nor that
of Y3+, Gd3+ and La3+ in six human C cells (data not shown).
In some preparations, applying extreme transmembrane potentials transiently
relieves block of ion channels by charged antagonists (Swandulla & Armstrong,
A
B
O
|
st
/
L
40 ms
LO
2 ms
C
8
6
5
4
8
* Control
E 3
2 -o 1 MLa3+
6
-200
0
0
8
-160
-120
-80
Repolarization potential (mV)
Fig. 4. Effect of M3+ on T-type VGCC gating. A, inactivation kinetics: scaled current traces
recorded at test potentials of -20 mV are shown beginning with the point of fastest
inactivation. The vertical scale bar applies to the control record (0 Gd3+). Currents recorded
after addition of 100 and 500 nM Gd3+ are enlarged 1P25 and 3-73 times, respectively. B,
deactivation kinetics: scaled tails, recorded at -80 mV, are shown from the point of the
fastest deactivation. The vertical scale bar applies to the control record (0 y3+). The scaling
factors for tail currents recorded upon addition of 50 and 250 nm Y3+ are 1P33 and 4 03,
respectively. C, effect of La3+ on deactivation kinetics in a human C cell: deactivation time
constants (TD), obtained by fitting of raw data records, have been plotted in respect to the
repolarization potential at which tails were recorded. *, control; 0, 1 /,M La3+.
1989; Thevenod & Jones, 1992). For positively charged pore blockers, extreme
hyperpolarization appears to attract the trapped cation into the cell while strong
depolarization expels it to the extracellular space. To determine whether block of IT
in human C cells by Y3+ and La3+ may be transiently relieved at positive potentials,
we measured tail currents at -80 mV after depolarizing steps to potentials as
positive as +80 mV. To find if block was relieved at negative voltages, tail currents
were measured at potentials between -50 and -200 mV, following an activating
test pulse to 0 mV (Fig. 6, insets). Since the transient unblocking and/or reblocking
upon change in membrane potential may occur rapidly (less than 1 ms), an effort
was made to achieve fast voltage clamp. Using small spherical human C cells (cell
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METAL CATION BLOCK OF T-TYPE CALCIUM CHANNELS 647
capacitance t 10 pF) and low resistance patch electrodes, which during recording
resulted in access resistances of 2-3 MQ, useful current measurements were
obtained at times of > 80 ,us (at least 4 time constants of voltage clamp, TVc) after
switching to the repolarization potential. Tail currents were measured at least
B
A
0*20
Control
A
300
|
0.
j
='
E
200
~~~~0*15
A
A
A__;*--AA
~~~~~~~~~oA
A
A
3 0*10 .
Co
&
c
100
0L05
2uMMLaM
-90 -80 -70 -60 -50
Conditioning potential (mV)
0
2
1
-1
0
log stimulation frequency (log Hz)
Fig. 5. Voltage and use independence of T-type VGCC block by La3+ in rat C cells. A, effect
of 2 /M La3+ in rat C cells. IT was activated by voltage steps to -15 mV after applying 20 s
conditioning pulses to various potentials between -100 and -40 mV. Availability curves
(or steady-state inactivation curves) were best fit of data from a single cell to a Boltzmann
expression of the form:
IT= I jAX/{1 + exp[(V.-Vj)/k]},
where IT is measured current amplitude, 'MAX is the maximal IT, VC is conditioning
potential, Vi is the potential at which half of channels are available for activation and k is
the slope factor. Vi is -62-8 + 0 3 mV for the control and -63-9 + 0 5 for 2 /M La'+. B, usedependent block of IT by La'+. Data points represent the fraction of IT remaining in a rat C
cell after steady-state block by 2 /M La'+. Data points are the fraction of IT remaining
after block, relative to the control value at the same stimulation frequency. Holding
potential was -80 mV, test potential was -25 mV. Linear regression line through the data
points has a slope not significantly different from zero.
90 jus after tail inactivation in control conditions and in the presence of Y3+ or La3+.
Neither extreme depolarization (n = 6 for 0'2 to 2-5 /mM Y3+; n = 2 for 1 /M La3+) nor
extreme repolarization (n = 2 for both 0 2 #M Y` and 1 AM La3+) significantly
relieved the block by Y3+ or La3+ (Fig. 6).
Block of IT by divalent nickel cation (Ni2+)
Although Ni2` is widely used as a preferential antagonist of T-type VGCC,
properties of its blocking action apart from concentration dependence have not
been described. Ni2` reversibly blocked IT in rat C cells with an IC50 of 5-8 + 0 5 EM
and Hill slope (N) significantly less than 1 (0-69 + 0 05) (Fig. 7 A), and in human C
cells (n > 5, not shown) with IC50 of 5-45 + 0 5 /M and Hill slope of -0-62 + 0 04. In
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B. MLINAR AND J. ENYEART
648
rat C cells, blocking potency with respect to equipotential control was not affected
by changing of conditioning potentials in the range from -95 to -45 mV (n = 3;
Fig. 7 B). Voltage dependence of block was studied over a wider range of potentials
in human C cells, with experimental protocols the same as those used in Fig. 6.
A
B
Depolarization potential (mV)
54iO
-25
25
-50
_
_
.
SP--rEM, ER
_
,
0O 000[
*
y0000003
Depolarization potential (mV)
25
50
-25
-50
,
A
A
°°
_
a
CL
* -200-I-
<
A
A
U
A
I--
.
a
AL
AALA&,
L.a3+
A
-200
A
0
L-
CZ
I
A
lU
C.)
e~~~~~~~~~~~~~~~~~~~~~~~~~
-600-
.
AA
AA r A
-400 nAA
Control
m
Control *.*
C
Repolarization potential (mV)
-100
-50
-200
-150
D
-200
0
Repolarization potential (mV)
-50
-150
-100
0
0 0
0 0
0 0
0
0
0 0
y3+
0
**
0
a
0
00
0
0o
-
La3+0
I--
-250
0
0
A
-200 + 6
-
0 0
0
o
.0q1)
0
-
0
-500
-400]+
._
HL-
*0. 00
--Control
**
-750
0
-
Control
-600 +
Fig. 6. Effect of extreme voltages on block of IT by Y3+ and La3". A and B, tail currents
recorded at a repolarization potential of -80 mV after activation by 10 5 ms steps to
various potentials between -60 to + 50 mV before and after block by 200 nM Y3+ (A) or
1 #uM La3` (B). Cut-off frequency of analogue filter was 12 kHz and sampling rate was
30 /s. Tvc was 35 ,us (A), and 31P5 /us (B). Tail current amplitudes are the average of 3
consecutive sample points recorded 120 to 180 ,s after repolarization. C and D, tail
currents were recorded at potentials between -50 and -200 mV after activation by
10-S5 ms depolarizing steps to 0 mV. Data points represent amplitudes of recorded tail
currents before and after block by 200 nm Y` (C) or 1 /am La3" (D). Sampling and filtering
specifications as in (A). TVC for C and D were 35 and 60 Ius respectively. Lag of averaged
sample points was 210-270 ,us.
were
Neither depolarization to + 80 mV (n = 6; Fig. 7C), nor repolarization to -200 mV
(n = 2; Fig. 7D) influenced the potency of IT block by 5 to 200 /LM Ni2+.
DISCUSSION
Mechanism of block of T-type VGCC by Y3+, La3+, lanthanides and Ni2+
The results presented here show that Y3+, La3+, lanthanides and Ni2+ block
current through T-type VGCC in a competitive and concentration-dependent
for on
lack
of effects on channel
of nanomolar
concentration
manner. Sufficiency
block,
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July 12,
2011
649
METAL CATION BLOCK OF T-TYPE CALCIUM CHANNELS
gating and notable antagonism between Ca2+ and the blockers favour ion pore
occlusion as the blocking mechanism for each of these cations. Blocking actions
through screening of negative surface charges, or by allosteric modulation seems
unlikely for the same reasons.
B
A
1.0
600
Control
(4)
c't 0° [
400-
E
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* *200| ^ ^ ^ ^
e
-8
-5
-6
-7
i*
CZ
>200
0-5
0~~~~~~~~~~~~~
-3
-4
-90
log concentration [Ni2-](log M)
C
.
in F 5A
A.
-80
-70
-60
-50
Conditioning potential (mV)
D
Repolarization potential (mV)
-50
:1
m
0a9
and-7 -100
te c -200 -150
+ 0 1 mV ao
Depolarization potential (mV)
-50
|
e
-25.
25
iw
50
A
~A
AA
Z
inhibition curvein
-1 00
A
AA
A
A
A
A
(O). C andiD, theeffectof25
-200a3
o A
A
0
~~~~~~~~~~~~~~~~~~-400-
Control
A Control
A AA A A*Cotl
A
~~~~~~~~~Ni2+
0
A
r psAA
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~A
H
o0
-200c
0
AA
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~~~~~~~~~-6001
els xeiet eepromda
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cotrl
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block of ITieti
of
block
7.
Nis.
A,
by Nicurn
concentration-dependent
IT
Properties
by
Fig.
to 2418 cu fe h eoaiainse,wr vrgdt
inning~~~~~~~~~~~
C cells. Inhibition curve with an IC50 of 5o65 + 0a84 and Hill slope of -0s69 + 005 was
obtained using procedures as described in Fig. 2. Experimental replicates, n, are given in
parentheses. B, effect of 5/ilm Ni1on IT availability in a rat C cell. Experimental procedure
as in Fig. 5 A. VI was -69-5 + 0-1 mV for the control (@) and -71-09 + 0-2 mV for the
Nil+ (&aC; control, A) and 5 thm
inhibition curve in Ni` (0). C and D, the effect of 25 lm
Ni2+ (K0,D; control, *) on tail currents in human C cells. Experiments were performed as
described in the legend of Fig. 6. In C, TV was 47 ss, 3 consecutive sample points,
beginning 180 to 240 /ss after the repolarization step, were averaged to give tail current
amplitudes. In D, -rv = 36 ,ss and the lag was 120 to 180 /ss.
In order to pass through an ion channel, a permeant ion must bind to at least
one, and probably two or more binding sites located in the permeation pathway
(Hess & Tsien, 1984; Hess, Lansman & Tsien, 1986). Inorganic channel blockers and
permeant ions appear to compete for common binding sites at the channel.
Therefore, inorganic blockers are useful tools for studying properties of binding
sites (see Lester, 1991; Hille, 1992 for references and discussion). The voltageindependent block of T-type VGCC by y3 La3+and lanthanides, described in this
study, implies that block occurs by their binding to a site which is outside the
M3+
the
small(jp.physoc.org
field. However,
membrane electric
changes in) bypotency
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from J Physiol
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July
12, 2011antagonists
650
B. MLINAR AND J. ENYEART
at different potentials cannot be ruled out because of difficulties in measuring small
( < 50 pA) tail currents at high cut-off frequencies (e.g. Fig. 6D). The inability of
extreme hyperpolarizing pulses to even transiently alleviate block indicates that
Y3+, La3+ and lanthanides, as well as Ni2` cannot pass through T-type VGCC, and
thus represent non-permeant T-type VGCC antagonists.
Inability of Y3+, La3+ and Ni3+ to change macroscopic deactivation and
inactivation kinetics suggests that T-type VGCC can normally close or inactivate
when occupied with one of the antagonists. This, in turn, implies that under
physiological conditions, in the absence of antagonists, at least one Ca2+ ion stays
trapped in the closed or inactivated channel, respectively. Swandulla & Armstrong
(1989) discussed the possibility that binding and unbinding the Ca2+ ion to the
channel pore participates in gating of ion channels.
Inferences about structure of the T-type VGCC binding site
Cationic radius is an important variable that determines the affinity and rate of
ion interactions with protein-binding sites (Tew, 1977; Tam & Williams, 1985).
Lanthanides, La3+ and y3+ are the most useful metal cations for studying Ca2+dependent processes because they share similar chemical properties and ionic radii
with Ca21 (Nieboer, 1975; dos Remedios, 1981). Assuming that the IC50s which we
measured provide a good approximation of the lanthanide antagonists' affinity for
the T-type VGCC, our results show a gradual decrease in the affinity of larger
lanthanides for the binding site. Such an affinity sequence resembles those of
lanthanide complexes with EGTA, EDTA and especially acetylacetonate, as well as
those reported for interactions of lanthanides with several proteins. However,
different affinity sequences for proteins and various biological preparations, with
maximal affinity for larger elements or for those in the middle of the lanthanide
series, have more often been observed (for references see Nieboer, 1975; dos
Remedios, 1981; Evans, 1992). The interpretation of ionic radius-dependent
interactions of lanthanides with Ca2+/lanthanide-binding sites of proteins according
to a scheme proposed by Tew (1977), explains the affinity sequence on the basis of
differences between the free energy of hydration of the cation and the energy of
interaction between the cation and the negative binding site (see dos Remedios,
1981; Evans, 1990; Lansman, 1990 and references cited therein for more detailed
discussion).
Comparison with previous studies of Y3+, La3+ and lanthanide effect on VGCC
Quantitative description of T-type VGCC block by La3+ has been reported for
mouse neuroblastoma cells (IC50 = 1-5 #M, in 50 mm Ba3+, Narahashi et al. 1987),
rat hypothalamic neurones (IC50 = 0 7 /M, in 10 mM Ca2 , Akaike et al. 1989) and
rat aorta smooth muscle cells (IC50 = 0-6 /M, in 20 mm Ca2+, Akaike et al. 1989).
IC50S for y3+ and lanthanide block of T-type VGCC have not been reported.
In comparing our results to Lansmans' study (1990) of lanthanide block of current
through L-type VGCC, we have noted the following differences: (1) the potency of
steady-state block by lanthanides is voltage independent for T-type VGCC, but
shows characteristics of open channel block for L-type VGCC; (2) repolarization of
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METAL CATION BLOCK OF T-TYPE CALCIUM CHANNELS
651
open channels to negative potentials can force lanthanides through L-type, but not
T-type VGCC; (3) larger lanthanides block L-type channels more potently than
those with smaller ionic radii. The inverse relationship holds for lanthanidemediated block of T-type VGCC.
High voltage-activated N- and P-type Ca2" channels are prominent in nerve
terminals of the CNS (Turner, Adams & Dunlap, 1992). A series of lanthanides were
shown to inhibit voltage-dependent Ca2" influx into rat brain synaptosomes
according to a potency sequence resembling that observed for block of L-type VGCC
(Nachsen, 1984). Differences between high voltage-activated VGCC (L-, N- and Ptype) and low voltage-activated T-type VGCC with respect to sensitivity to
lanthanides of different radii suggest corresponding differences in the structure of
their respective Ca2+/M3" binding sites. Surprisingly, with respect to potency
(IC50 2 /M in 2 mm Ca2", Rechling & MacDermot, 1992) and lack of voltage
dependence, block of T-type Ca2" channels by La3" resembles inhibition of the
NMDA ligand-gated ion channel.
This work was supported by National Institute of Diabetes and Digestive and Kidney grant
DK-40131 to J.J.E.
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