A Raman spectroscopic study of arsenite and thioarsenite species in
aqueous solution at 25 uC
Scott A. Wood,*a C. Drew Taitb and David R. Janeckyb
a
Department of Geological Sciences, Box 443022, University of Idaho, Moscow, ID
83844-3022, USA. E-mail: swood@uidaho.edu
b
Chemical Science and Technology Division, Los Alamos National Laboratory, Los Alamos,
NM 87545, USA
Article
Received 17th December 2001, Accepted 19th February 2002
Published on the Web 26th February 2002 First published as an Advanced Article on the web
The Raman spectra of thioarsenite and arsenite species in aqueous solution were obtained at room
temperature. Solutions at constant SAs 1 SS of 0.1 and 0.5 mol kg21 were prepared with various SS/SAs
ratios (0.1–9.0) and pH values (y7–13.2). Our data suggest that the speciation of As under the conditions
investigated is more complicated than previously thought. The Raman measurements offer evidence for at least
six separate S-bearing As species whose principal bands are centered near 365, 385, 390, 400, 415 and
420 cm21. The data suggest that at least two different species may give rise to bands at 385 cm21, bringing the
probable minimum number of species to seven. Several additional species are possible but could not be resolved
definitively. In general, the relative proportions of these species are dependent on total As concentration,
SS/SAs ratio and pH. At very low SS/SAs ratios we also observe Raman bands attributable to the dissociation
products of H3AsO3(aq). Although we were unable to assign precise stoichiometries for the various thioarsenite
species, we were able to map out general pH and SS/SAs conditions under which the various thioarsenite and
arsenite species are predominant. This study provides a basis for more detailed Raman spectroscopic and other
types of investigations of the nature of thioarsenite species.
1. Introduction
In moderately oxidizing to moderately reducing hydrothermal
solutions the species H3AsO30 or one of its deprotonated
equivalents generally is considered to be the dominant form of
dissolved arsenic.1–9 The As(V) species H3AsO40 or one of its
dissociation products may be predominant under near-surface,
oxidizing conditions.6,10 Under extremely reducing conditions,
dissolved arsine gas, AsH3(aq), may be an important species.5
However, at low temperatures, in near-neutral to alkaline,
sulfide-rich solutions, some form of thioarsenite [As(III)-sulfide]
species is most likely responsible for the hydrothermal transport of As.2,7,11–24
The exact stoichiometry of the important thioarsenite complexes remains controversial even under standard conditions
despite intense investigation. Spycher and Reed21,25,26 proposed that the As(III)-sulfide species most consistent with the
available literature at the time were the trimers H3As3S60,
H2As3S62 and HAs3S622. Krupp27,28 emphasized some of
the drawbacks of the approach of Spycher and Reed21 and
favored, in analogy with Sb, some form of the dimer (H2As2S4,
HAs2S42, or As2S422). Subsequently, new experimental solubility studies have been interpreted both in terms of the trimer
H2As3S627,23 and the dimeric species.22 Using EXAFS, Raman
spectroscopy and ab initio molecular orbital calculations, Helz
and coworkers24 have concluded that, in solutions undersaturated with respect to either orpiment or amorphous As2S3,
monomeric species (i.e., H2AsS32 and HAsS322, actually
formulated24 as AsS(SH)22 and AsS2(SH)22) are predominant.
However, Helz and coworkers24 reinterpret previous solubility
experiments7,22,23 and suggest that, at saturation, the predominant species are the trimer As3S4(SH)22 and the monomer AsS(OH)(SH)2. Thus, the results of Helz et al.24 strongly
support the possibility that the degree of polymerization of
thioarsenite species depends on the total As concentration in
solution and that there may be a progression from monomers
through dimers to trimers (to possibly higher polymers) as
DOI: 10.1039/b111453k
total As increases towards saturation with respect to an arsenic
sulfide phase. Krupp29 has suggested the possibility of a similar
progression for thioantimony species.
It is apparent from this brief review of the literature that
additional work is required to determine unambiguously the
stoichiometries and thermodynamic stabilities of thioarsenite
species. In this paper we present results of Raman spectroscopic measurements conducted on thioarsenite and arsenite
species near 25 uC in near-neutral to alkaline solutions. These
results are preliminary in nature but they do provide some
additional constraints on the nature of thioarsenite species and
the conditions under which they are transformed into arsenite
species.
2. Experimental
All solution preparation and manipulation was conducted in a
low-oxygen, nitrogen-filled glove bag in order to minimize oxidation. Solutions were prepared in which the total sulfur to
total arsenic ratio (SS/SAs) varied but the sum of the total
arsenic and sulfide concentrations was held constant. A NaHS
stock solution was prepared by bubbling H2S gas through a
solution of known NaOH concentration until measured pH
was constant with time. A sodium arsenite solution was prepared by dissolving reagent-grade As2O3(s) in a NaOH solution. For a given set of experiments, the stock solutions of
NaHS had the same sulfide concentration as the concentration
of As in the stock sodium arsenite solutions. These two stock
solutions were then mixed in various proportions to obtain
solutions with SS/SAs ratios of 9, 5.25, 4, 3.17, 1.94, 1.00,
0.515, 0.25 and 0.11. Two different sets of experiments were
carried out: one in which SAs 1 SS ~ 0.1 mol kg21, and one in
which SAs 1 SS ~ 0.5 mol kg21. In each set of experiments,
the pH of each of the solutions was adjusted to a constant value
using solutions of NaOH or HCl. A pH range from 13.2 to
approximately 7 was investigated. More acidic solutions could
Geochem. Trans., 2002, 3(4) 31-39
This journal is # The Royal Society of Chemistry and the Division of Geochemistry of the American Chemical Society 2002
not be studied owing to precipitation of arsenic sulfide.
Measurements of pH were conducted using an Orion Research
Meter (Model SA250) with a Ross combination electrode,
standardized against NIST pH buffers. An aliquot of each
solution was placed in a glass NMR tube, and the tube was
capped and sealed with Parafilm. The Raman spectrum of each
solution was acquired at room temperature within 1–2 hours
after being sealed in the NMR tube. Owing to the preliminary
nature of the experiments, no internal standards were
employed. Thus, the spectra could only be treated in a semiquantitative manner.
The Raman instrumentation has been described in detail
previously.30,31 Raman spectra were excited using the 514-nm
line of a Spectra Physics Ar-ion laser (model 2025-05) focused
onto the sample with a cylindrical lens to match the monochromator slit. The laser delivered approximately 400 mW of power
to the sample. The Raman scattering was viewed in a 135u
backscattering configuration, with the scattered light collimated with a fast plano-convex lens (fl 1.3, planar side toward
focused spot on sample) and then subsequently focused onto
the slit of the monochromator (SPEX Triplemate, fl 6.3 input
optics) with a second plano-convex f-matching lens (fl 6.5,
planar side towards monochromator).The first two stages of
the monochromator were locked in an additive–subtractive
mode and function as a tunable bandpass filter, especially
against the Rayleigh-scattered photons. The final stage dispersed the inelastically scattered photons across a Princeton
Applied Research model 1420 intensified silicon diode-array
detector. The slit width of the monochromator was set at
140 mm. In general, 10 scans, each with a 10 s integration time,
were co-added to improve the signal-to-noise ratio. Spectra
were calibrated against standard peaks of toluene in methanol
and the emission lines of a neon lamp. Spectral data were saved
as ascii files. Attempts to reduce the spectra in the manner
described by Brooker et al.32 or to use PeakFit (Jandel Scientific) to obtain quantitative band parameters (peak maxima,
band widths, and band areas) were frustrated by the variable
nature of the fluorescence of the NMR tube. Thus, only the raw
spectra are presented here. However, PeakFit was employed to
help identify the peaks of obvious Raman bands.
Fig. 1 Raman spectrum of deionized water in a glass NMR tube. Note
broad bands from approximately 335 to 550 cm21 and 750 to 850 cm21.
There may also be a less intense broad band near 600 cm21.
of the solutions with higher SS/SAs ratios, but we did not
routinely monitor this area of the spectrum. Attention was
focused on the wavenumber range from 250 to 900 cm21 where
bands for thioarsenite and arsenite species occur.
Fig. 2 contains the spectra of solutions with SS 1 SAs ~
0.1 mol kg21 at pH ~ 13.2 for various SS/SAs ratios. At a
SS/SAs ratio of 9, a prominent, narrow band centered at
approximately 385 cm21 is evident, together with a lowintensity shoulder on its high-wavenumber side. Upon a
decrease in SS/SAs ratio to 5.25, another narrow band, centered near 400 cm21 can be seen to emerge at the expense of the
band at 385 cm21. The low-intensity shoulder on the highwavenumber side does not appear to undergo much of a
change as SS/SAs decreases. By a SS/SAs ratio of 4, the band
at 385 cm21 has disappeared. Between SS/SAs ~ 4 and SS/
SAs ~ 0.25, the band at 400 cm21 loses intensity, while a band
3. Results
Fig. 1 shows the background spectrum due to deionized water
and the glass NMR tubes. The major features of the background are: (1) the Rayleigh wing descending from 250 cm21;
(2) a broad, moderately intense, asymmetric band with a peak
near 480 cm21; (3) a broad, weak band centered at 800 cm21; and
(4) a very weak, broad band near 600 cm21. Water has libra- tion
bands owing to hydrogen bonding at 450 and 789 cm21 which
may contribute to the two most prominent broad bands.33
However, the broad band near 480 cm21 is far more intense
than usually observed in pure water, and probably is a result of
fluorescence due to impurities in the glass NMR tube. The
bands for thioarsenite species all fall on the low-wavenumber
side of the broad band near 480 cm21 which, combined with the
proximity of the Rayleigh wing, hindered quantitative bandfitting.
Fig. 2–6 contain spectra for solutions containing both S and
As. Each figure shows the spectra for a given pH and SS 1 SAs
value as a function of SS/SAs ratio. For the convenience of the
reader, some of these spectra are replotted in a slightly different
manner in Fig. 7–11. In the latter, each figure shows the spectra
for SS 1 SAs ~ 0.1 and a given SS/SAs ratio as a function of
pH.
In our solutions with relatively high SS/SAs ratios, significant bisulfide (HS2) is expected to be present. This species
should yield a narrow band at about 2574 cm21.34 At an early
stage of this study, we verified that this band is present in some
Geochem. Trans., 2002, 3(4) 31-39
Fig. 2 Raman spectra of arsenic sulfide solutions at pH ~ 13.2 and
SAs 1 SS ~ 0.1 mol kg21 H2O as a function of SS/SAs ratio.
Fig. 3 Raman spectra of arsenic sulfide solutions at pH ~ 12.6 and
SAs 1 SS ~ 0.1 mol kg21 H2O as a function of SS/SAs ratio.
Fig. 5 Raman spectra of arsenic sulfide solutions at pH ~ 8.4–8.5 and
SAs 1 SS ~ 0.1 mol kg21 H2O as a function of SS/SAs ratio.
centered near 420 cm21 becomes relatively more prominent
(although it never becomes very intense), until it too loses
intensity. Meanwhile broad bands near 600 and 800 cm21 grow
in intensity. At SS/SAs ~ 0.11, the bands in the 350–450 cm21
range have disappeared, leaving only the broad band observed
in the background spectrum, and bands of increased intensity
near 600 and 800 cm21. As indicated below, the increase in
intensity of the latter bands can be attributed to the appearance
of an arsenite species. It is possible that the shoulder observed
on the high-wavenumber side of the bands at 385 and 400 cm21
at higher SS/SAs ratios is the same as the band at 420 cm21
visible at lower SS/SAs ratios, inasmuch as there continually
appears to be some intensity at around 420 cm21 throughout
the entire range of SS/SAs ratios investigated. The bands at
385, 400 and 420 cm21 each appear to belong to separate
species as their intensities do not change in a concerted manner
as SS/SAs is varied. Thus, at pH ~ 13.2 and SS 1 SAs ~
0.1 mol kg21 at least three separate species exist, in addition to
that responsible for the bands at 600 and 800 cm21.
Raman spectra for solutions with SS 1 SAs ~ 0.1 mol kg21
Fig. 6 Raman spectra of arsenic sulfide solutions at pH ~ 12.6 and
SAs 1 SS ~ 0.5 mol kg21 H2O as a function of SS/SAs ratio.
Fig. 4 Raman spectra of arsenic sulfide solutions at pH ~ 10.5 and
SAs 1 SS ~ 0.1 mol kg21 H2O as a function of SS/SAs ratio.
Fig. 7 Raman spectra of arsenic sulfide solutions at SS/SAs ~ 9.0 and
SAs 1 SS ~ 0.1 mol kg21 H2O as a function of pH.
Geochem. Trans., 2002, 3(4) 31-39
Fig. 8 Raman spectra of arsenic sulfide solutions at SS/SAs ~ 4.0 and
SAs 1 SS ~ 0.1 mol kg21 H2O as a function of pH.
Fig. 9 Raman spectra of arsenic sulfide solutions at SS/SAs ~ 1.94
and SAs 1 SS ~ 0.1 mol kg21 H2O as a function of pH.
and pH ~ 12.6 are shown in Fig. 3. Only one prominent band
is noted above background in the range 350–450 cm21, but the
maximum of this band appears to shift from about 385 cm21 to
397 cm21 as the SS/SAs ratio varies from 9 to 0.25. The band is
asymmetric to the high-wavenumber side, and there may also
be some intensity above background on the low-wavenumber
side. All of these observations suggest the presence of more
Fig. 10 Raman spectra of arsenic sulfide solutions at SS/SAs ~ 0.515
and SAs 1 SS ~ 0.1 mol kg21 H2O as a function of pH.
Geochem. Trans., 2002, 3(4) 31-39
Fig. 11 Raman spectra of arsenic sulfide solutions at SS/SAs ~ 0.25
and SAs 1 SS ~ 0.1 mol kg21 H2O as a function of pH.
than one component to the band. We interpret these observations in terms of the presence of three strongly overlapping
bands, the same ones at 385 and 400 cm21 observed at pH ~
13.2, and an additional band at approximately 390 cm21. The
intensity of these bands varies with SS/SAs in such a manner
that the apparent peak of the band envelope shifts to higher
wavenumbers. As at pH ~ 13.2, as the SS/SAs ratio decreases,
bands near 600 and 800 cm21 increase in relative intensity,
suggesting the formation of arsenite species.
The band near 385 cm21, observed in both Fig. 2 and 3, is
also observed in solutions with high SS/SAs at SS 1 SAs ~
0.1 mol kg21 and pH ~ 10.5 (Fig. 4). In addition, at low
SS/SAs ratios a band near 415 cm21 is present, and this band
increases in intensity with respect to the band at 385 cm21 as
SS/SAs decreases. A broad, low-intensity band appears to be
present at about 330 cm21 at SS/SAs ~ 9. This band increases
and then seems to remain constant in intensity from SS/SAs ~
5.25 to SS/SAs ~ 3.17, and then decreases in intensity and
disappears by SS/SAs ~ 0.52. The band near 385 cm21
remains visible until SS/SAs ~ 1, after which it disappears.
The band at 415 cm21 attains a maximum intensity at
SS/SAs ~ 4, and then decreases in intensity until it disappears
completely at SS/SAs v 0.25. The peak position of this band
appears to change slightly from 415 to 412 cm21 with
decreasing SS/SAs ratio. A very low-intensity band appears
at about 435 cm21 at SS/SAs ~ 1.0 but disappears at SS/SAs
v 0.25. At about SS/SAs ~ 1.94, bands begin to appear at 600
and 790 cm21 and these grow in intensity to reach a maximum
at the lowest SS/SAs (0.11) value measured. At SS/SAs v
0.515, a weak, broad band also appears near 700 cm21. The
band near 415 cm21 could possibly be interpreted to result
from the same species responsible for the band observed at
420 cm21 at pH ~ 13.2. However, we reject this interpretation
for the following reasons: (1) A difference of 5–8 cm21 is
outside the uncertainty in identification of band peak positions
for such a relatively narrow band; (2) the intensities of the
bands at 330 and 415 cm21 appear to be correlated, yet no
corresponding band at 330 cm21 was observed to occur with
the band at 420 cm21 at pH ~ 13.2 (although admittedly the
intensity of the latter band is low and so a band at 330 cm21
might not be detectable even if present); and (3) a band near
415–420 cm21 was not observed at pH ~ 12.6, and it is highly
unlikely that a species present at pH ~ 13.2 would first
disappear and then reappear as pH decreases. Thus, we
conclude that the band at 415 cm21 represents a previously
unidentified As species. The apparent shift of this band to lower
wavenumbers with decreasing SS/SAs ratios could be an
indication of overlap of bands of two different species, the
relative intensities of which vary with SS/SAs ratio. The
apparent band at 435 cm21 may represent yet another As
species, but we are less confident of its existence because of the
very low observed intensity of this band. Thus, at least one,
possibly more, new species appear as pH is decreased to 10.5.
The spectra at low wavenumbers for solutions with SS 1
SAs ~ 0.1 mol kg21 and pH ~ 8.4–8.5 at high SS/SAs (Fig. 5)
are very similar to those in Fig. 4 (pH ~ 10.5). Bands are
observed near 330, 385 and 415 cm21, and the relative intensities of these bands at a given value of SS/SAs do not appear
to be strongly dependent on pH. In fact, given the fact that pH
differed by 2 units in the solutions the spectra of which are
shown in Fig. 4 and 5, the lack of change in relative intensities
of the bands in the region 300–450 cm21 is particularly striking.
On the other hand, the peak of the band near 415 cm21 actually
shifts from around 419 cm21 at SS/SAs ~ 9.0, to a minimum
of 412 cm21 at SS/SAs ~ 1.0, and then back up to 415 cm21 at
SS/SAs ~ 0.52. Once again, these apparent shifts may be an
indication of the presence of two or more species with
overlapping bands. In the spectral region above 500 cm21
there are a number of changes that occur at low SS/SAs on
going from pH ~ 10.5 to pH ~ 8.5. First, the intensity of the
bands near 600 and 790 cm21 are substantially reduced at the
lower pH values. Second, a rather sharp band appears near
702 cm21 and increases in intensity towards a maximum at
SS/SAs ~ 0.11. Finally, a low, broad shoulder appears on the
low-wavenumber side of the latter peak at approximately
650 cm21.
Finally, Fig. 6 shows the spectra obtained for solutions with
SAs 1 SS ~ 0.5 mol kg21 and pH ~ 12.5–12.8. At SS/SAs ~
9.0, these solutions exhibit an intense band with a peak near
384 cm21 and asymmetric to the low-wavenumber side. Very
weak peaks are also apparent at about 308 and 344 cm21. With
decreasing SS/SAs ratio, the intense band near 384 cm21
appears to shift to 389 cm21, and a prominent shoulder
develops at ~365 cm21. The shoulder seems to maintain its
intensity relative to the more intense band from SS/SAs ~ 4.0
to 1.94. At lower SS/SAs ratios, both the shoulder near 365
and the main peak near 389 cm21 decrease in intensity, but the
intensity of the shoulder decreases faster than that of the main
peak. At SS/SAs ~ 0.52 the shoulder has all but disappeared,
but the main band remains markedly asymmetric. This main
band is still present at SS/SAs ~ 0.25, but disappears completely at SS/SAs ~ 0. Thus, at this higher value of SAs 1 SS,
at least one new species, one with a band near 365 cm21, has
been identified. At this point is not clear whether the band near
308 cm21 represents a separate species or is a band attributable
to the same species responsible for the band at 365 cm21.
However, the broadness of the spectral envelope in the region
350–400 cm21 is permissive of the existence of several additional species. Very broad bands near 600 and 800 cm21, attributable to arsenite species, are clearly present at SS/SAs ratios
as high as 1.94, and possibly 3.17. These are essentially the same
bands observed in solutions with SS 1 SAs ~ 0.1 mol kg21 and
pH ~ 12.6 at low SS/SAs, indicating that the arsenite species
do not form polynuclear species under the conditions investigated here. This conclusion is consistent with the Raman
results of Pokrovski et al.9 which showed that H3AsO30 does
not form polynuclear species until SAs w 1 mol kg21.
Fig. 7 shows the spectra at SS 1 SAs ~ 0.1 and SS/SAs ~
9.0 as a function of pH. This figure shows essentially the same
data as seen in Fig. 2–6, with the addition of the spectrum of a
solution with pH ~ 6.75, the only solution of this pH for which
we were able to obtain a spectrum. As discussed above, a band
near 385 cm21 persists at all pH values at the given values of
SS 1 SAs and SS/SAs. Moreover, a band near 415 cm21
(accompanied by a band at 330 cm21) appears starting at
pH ~ 10.5 and persists to the lowest pH investigated.
Spectra for SS 1 SAs ~ 0.1 and SS/SAs ~ 4.0 as a function
of pH are compiled in Fig. 8. Again, these data were depicted in
previous figures, with the exception of an additional spectrum
collected at pH ~ 8.0. As noted previously, a band occurs at
about 400 cm21 at pH ~ 13.2, but by pH ~ 12.6, the latter is
replaced by a band at y390 cm21. At pH values between 10.5
and 8.0, bands occur at 330, 385 and 413 cm21, the relative
intensities of which do not change much over a range of 2.5 pH
units. Fig. 9 shows similar spectral features at SS/SAs ~
1.94 to those in Fig. 8, although at pH ~ 13.2 the band near
400 cm21 seems to have a more prominent shoulder on its highwavenumber side. Also, in Fig. 9 at pH ~ 8.4–8.5, a band
appears at y700 cm21 as well.
At SS 1 SAs ~ 0.1 and SS/SAs ~ 0.515, the spectral
features between 300 and 500 cm21 (other than the background
band) have very low intensities and are not very distinct
(Fig. 10). Between pH ~ 10.5 and pH ~ 8.4–8.5, there is a
notable change above 600 cm21, in which the broad band near
800 cm21 gives way to a sharper band near 700 cm21 that has a
shoulder on the low-wavenumber side. At SS 1 SAs ~ 0.1 and
SS/SAs ~ 0.25 (Fig. 11), the spectra are very similar to those in
Fig. 10, except that there is almost no intensity above
background from 300 and 500 cm21. Also, at pH ~ 8.1 a
very broad band appears from about 300 to 420 cm21. This
band most likely represents scattering from solid particles
precipitated on decrease of pH.
The Raman bands enumerated above are the minimum
number of bands present. There may well be other bands representing additional species, that cannot be resolved without
quantitative band fitting. Indeed, results of attempts to perform
such band fitting support the likely presence of other species.
However, owing primarily to the presence and variable
intensity of the broad fluorescence band, we found it impossible
to fit the observed spectral data with bands that exhibited
systematic peak positions, intensities and peak widths as a
function of pH and SS/SAs. Nevertheless, our results do
indicate the presence of at least 6–8 separate S-bearing As
species. It seems clear that separate species are responsible for
bands near 365, 385, 390, 400, 415 (330) and 420 cm21. In
addition, more tentatively identified bands at 308, 344 and
435 cm21 may represent additional species (Table 1).
4. Discussion
Arsenite species
At low SS/SAs ratios, bands appear in the spectral region from
600 to 800 cm21 which may be attributed to S-free, As(III)
species (i.e., arsenites). Loehr and Plane35 have assigned
Raman bands to the various arsenite species, and these
assignments are given in Table 2. In Fig. 12, the distribution
of arsenite species as a function of pH at 25 uC and 1 bar is
illustrated.
The very broad bands observed near 600 and 800 cm21 at
pH ~ 13.2 (Fig. 2) are probably attributable to the species
HAsO322 and AsO332, which are present in nearly equal
proportions at pH ~ 13.2 (Fig. 12). The bands assigned by
Loehr and Plane35 to the first species are rather uncertain, but
overall our observations are consistent with their band assignments for these two most deprotonated arsenite species.
At pH ~ 12.6 (Fig. 3 and 11), the bands at 600 and 800 cm21
appear to be somewhat narrower than those at pH ~ 13.2,
consistent with the predominance of a single species, HAsO322,
at pH ~ 12.6 (Fig. 12). The positions of the bands are
consistent with those given by Loehr and Plane35 for HAsO322.
The species H2AsO32 is expected to be predominant at pH ~
10.5 according to the speciation diagram in Fig. 12. The
spectral assignment of Loehr and Plane35 would suggest the
most intense bands for this species to occur at 790, 610, and
570 cm21. We observe the most intense band at 790 cm21, and
a broad band that covers the region from 570 to 610 cm21,
consistent with the predominance of H2AsO32 at this pH
(Fig. 4 and 11). A weak band near 700 cm21 is apparent at low
Geochem. Trans., 2002, 3(4) 31-39
Table 1 Summary of observed Raman bands as a function of solution
composition
SS 1 SAs/
mol kg21
pH
SS/SAs
Peak positions/cm21
0.1
13.2
9.0
5.25
4.0
3.17
1.94
1.0
0.52
0.25
0.11
385
385, 400
400
400, 420, 600, 800
400, 420, 600, 800
400, 420, 600, 800
400, 420, 600, 800
420?, 600, 800
600, 800
0.1
12.6
9.0
5.25
4.0
3.17
1.94
1.0
0.52
0.25
0.11
385
389
390
391,
395,
395,
396,
397,
600,
9.0
5.25
4.0
3.17
1.94
1.0
0.52
0.25
0.11
385, 415
327, 385, 415
331, 385, 412
332, 384, 413
329, 384, 412, 600, 790
329, 384, 412, 435?, 600, 790
413, 435?, 600, 790
435?, 600, 700, 790
600, 700, 790
0.1
10.5
600,
600,
600,
600,
600,
800
800
800
800
800
800
0.1
8.4–8.5
9.0
5.25
4.0
3.17
1.94
1.0
0.52
0.25
0.11
328, 385, 419
329, 384, 418
331, 384, 413
331, 383, 413, 700
332, 382, 414, 701
334, 380, 412, 702
333, 384, 415, 702, 800?
415, y650sh, 702, 800
y650sh, 702, 800
0.1
6.75
8.0
8.1
9.0
4.0
0.25
y340, 385, 413
331, 384, 413
350, y650sh, 702, 800
0.5
12.6
9.0
4.0
3.17
1.94
1.0
0.52
0.25
0.11
308, 344, 384
308, 365sh, 389
308, 365sh, 389
308, 365sh,389, 600, 800
308, 365sh,389, 600, 800
308?, 390, 600, 800
370?, 390, 600, 800
600, 800
Fig. 12 Distribution of arsenite species as a function of pH at 25 uC and
1 bar. Thermodynamic data used to contruct this diagram (pK1 ~ 9.3;
pK2 ~ 12.1; pK3 ~ 13.4) are from Akinfiev et al.8
peak near 710 cm21, with a shoulder near 655 cm21. These
features are clearly observed in Fig. 5, 10 and 11. We also
observe a broad, low-intensity band near 790 cm21 at pH ~
8.5, which is consistent with the presence of a small proportion
of H2AsO32 at pH ~ 8.5 (Fig. 12).
Thioarsenite species
The bands observed in the region 300–450 cm21 can be
attributed to As–S bonds.24,36,37 These species may be simple
thioarsenites, or a mixed species such as AsO2S32, for example.
However, as all the bands in the region above 500 cm21 can be
accounted for by known arsenite species, we will assume that
simple thioarsenites are responsible for all the bands observed
in the region 300–450 cm21. The Raman spectra offer evidence
of the existence of at least 6–8 separate thioarsenite species over
the range of conditions investigated. To simplify discussion,
each major species (as inferred from the existence of a Raman
band exhibiting independent behavior as a function of pH and
SS/SAs) has been assigned an alphabetic designation as
enumerated in the caption to Fig. 13. Fig. 13 summarizes the
changes in species that occur as pH and SS/SAs ratio of the
solution change. This diagram is highly schematic in that the
positions and slopes of the boundaries among species are not
SS/SAs ratios in Fig. 4, which may be due to the presence of a
small proportion of H3AsO30 at pH ~ 10.5 (cf. Fig. 12).
Finally, at pH ¡ 8.5, the speciation diagram in Fig. 12
suggests that the completely protonated arsenite, H3AsO30,
should be predominant. For this species, Loehr and Plane,35
and also Pokrovski et al.,9 observed a very distinctive intense
Table 2 Compilation of Raman spectral assignments for arsenite
species from Loehr and Plane.35
Speciesa
Observed bands/cm21
H3AsO30 or As(OH)30
H2AsO32 or AsO(OH)22
HAsO322 or AsO2(OH)22
AsO332
710, 655
790, 610, 570, 370, 320
810?, 770?, 670-520?
752, 680, 340
a
Second formulations given are those preferred by Loehr and
Plane.35
Geochem. Trans., 2002, 3(4) 31-39
Fig. 13 Highly schematic diagram showing approximate predominance
fields of various As species as a function of pH and SS/SAs ratio for
SAs 1 SS ~ 0.1 mol kg21 H2O. With the exception of the boundaries
between the arsenite species (H3AsO30, H2AsO32, HAsO322, AsO332),
the slopes and the positions of the boundaries are highly uncertain. The
letters correspond to species responsible for observed Raman bands as
follows: A, 385 cm21; B, 400 cm21; C, 420 cm21; D, 390 cm21?; E, 330
and 415 cm21; and F, 385 cm21. At SAs 1 SS ~ 0.5 mol kg21 H2O an
additional species with a band at 360–370 cm21 joins species D at pH ~
12.6 and intermediate SS/SAs.
well constrained. Furthermore, we cannot make any definitive
statements regarding the exact stoichiometries of these thioarsenite species owing to a number of factors including: (1) the
interference by fluorescence of the glass tubes employed; (2)
absence of an internal standard; (3) lack of knowledge of the
scattering coefficients of the various species; and (4) the
relatively small number of bands that appear to be resolved for
each species. However, a number of conclusions can be made
regarding the chemical relationships among the various species
indicated by the Raman bands.
If we assume that there is only a single species exhibiting a
Raman band near 385 cm21, then the spectra show that species
A occurs at the highest SS/SAs ratios at all pH values. At pH
values of 13.2 and 12.6 and SS 1 SAs ~ 0.1 mol kg21, species
A appears to be the only or the greatly dominant thioarsenite
present at SS/SAs ~ 9, and other species begin to take its place
as SS/SAs ratio decreases. At pH values of 10.5 and 8.5 and
SS 1 SAs ~ 0.1 mol kg21, or at pH ~ 12.6 and SS 1 SAs ~
0.5 mol kg21, species A is present at SS/SAs ~ 9, but other
species are present in significant proportion as well. In these
cases also, species A is converted to other species as SS/SAs
decreases. If indeed the band near 385 cm21 arises from a single
species, then these results suggest that species A is stable over a
wide range of pH as long as SS/SAs is elevated.
Alternatively, the band near 385 cm21 may contain contributions from more than one species that happen to have a
Raman signal near this frequency. Some evidence for this
interpretation comes from Fig. 7. Here it is seen that the band
near 385 cm21 appears to decrease in intensity from pH ~ 13.2
to pH ~ 12.6, but then increase again from pH ~ 12.6 to
pH ~ 10.5. Although it is difficult to be certain whether these
apparent changes in intensity are real in the absence of an
internal standard, if so they suggest that possibly two different
species exist with bands near 385 cm21. Moreover, at pH ¡
10.5, bands at 385 and 415 cm21 coexist, and their intensities
seem to be quite insensitive to pH given a range of 3.75 units.
One interpretation of the spectra in Fig. 7 is that a species (A)
responsible for the band near 385 cm21 at pH w 10.5 gives way
to a second species (F) with a band near 385 cm21 and one with
a band near 415 cm21 (E), the latter two having a pHindependent relationship to one another. This would account
for the relative insensitivity of intensities of the two bands to
pH at pH ¡ 10.5, while at the same time the initial appearance
of the 415 cm21 band is pH-dependent. The apparent near
constant ratio of intensities of the bands 385 and 415 cm21
above pH ~ 10.5 could imply that both bands arise from a
single species. However, comparison of Fig. 7 and 8 shows that
the relative intensities of these two bands, although insensitive
to pH, are a strong function of the SS/SAs ratio, and thus
cannot belong to the same species.
Fig. 13 shows that at SS 1 SAs ~ 0.1 mol kg21 and pH
values of 8.5 and 10.5, species F converts to species E as the
SS/SAs ratio decreases. Moreover, as mentioned in the Results
section, the relative proportions of species F and E at a given
SS/SAs ratio do not appear to be strongly dependent on pH
(cf. Fig. 4, 5 and 7). These observations suggest that species A
must possess a higher SS/SAs ratio than species E, but that
there is no loss or gain of protons when species F converts to
species E. Thus, the boundary between F and E has been drawn
as a vertical, pH-independent boundary. On the other hand,
species E is apparently destabilized on increasing pH to 12.6
and higher, whereupon species D, B and C appear in turn.
Species D appears only at pH ~ 12.6 at intermediate SS/SAs
ratios. It is apparently present at both SS 1 SAs values
investigated. Because the appearance of species D relative to E
depends on the pH, species D may be a less protonated form of
species E. As the SS/SAs ratio continues to decrease, species D
is converted to species B.
At pH ~ 13.2, species A apparently converts directly to
species B with decreasing SS/SAs ratio, without first going
through species D, as was the case at pH ~ 12.6. With a further
decrease in SS/SAs ratio, species C appears at the expense of
species A and B.
Comparison to previous Raman studies of thioarsenite species
We are aware of only one other Raman study of aqueous
thioarsenites—that of Helz et al.24 The latter authors studied a
solution containing 1 M NaHS and 0.08 M As (SS/SAs ~
12.5) at pH 8.2 and 12 at 25 uC. They observed only three bands
over the range 150–750 cm21 at 325, 382 and 412 cm21. Upon
an increase in pH from 8.2 to 12, the relative intensity of the
band at 382 cm21 increased at the expense of the two bands at
325 and 412 cm21. Based on ab initio calculations, Helz et al.24
attributed the bands at 382 cm21 to the species AsS2(HS)22 and
the bands at 325 and 412 cm21 to the species AsS(HS)22, which
can be related to one another through the simple protonation
reaction:
AsS2(HS)22 1 H1 < AsS(HS)22
In contrast, Fig. 4, 5 and 7 indicate that the situation may not
be as simple. Like Helz et al.,24 we note three bands in the pH
range 8.5–10.5, at approximately 325, 382 and 412 cm21 (in our
case, 330, 385 and 415 cm21, i.e., our species F and E), and we
also observe generally a relative increase in the intensity of the
band at 385 cm21 with increasing pH at pH w 10.5 (the species
responsible for the bands at 330 and 415 cm21 disappears
completely by pH ~ 12.6). However, we further observe that,
at constant pH, the intensity of the band at 385 cm21 decreases
relative to that at 415 cm21 with decreasing SS/SAs ratio. This
observation indicates the existence of two species with different
SS/SAs ratios. Moreover, the relative intensities of the bands
at 385 and 415 cm21 at constant SS/SAs ratio do not change
very much with an increase of pH from 8.4 to 10.5 (Fig. 7).
Over such a large pH range, if the species responsible for these
bands were related simply by eqn. (1), we would expect much
larger changes in relative intensity, as the ratio of concentrations of the species would have to change by one order of
magnitude for each unit change in pH. Even in the work of
Helz et al.,24 the observed changes in relative intensities of the
bands near 382 and 412 cm21 as a function of pH were
relatively small, even though pH was varied by nearly 4 units. If
the only reaction occurring was reaction (1), there should have
been a much greater change in relative intensities with a change
in pH of this magnitude. Based on this reasoning, we are thus
forced to conclude that at least three species exist at high
SS/SAs over the pH range investigated. Two species (E and F),
which differ only in their SS/SAs ratio but not their degree of
protonation, exist at high SS/SAs and pH ¡ y10.5. As pH
increases above 10.5, species E and F together give way to a
single species A, which happens to yield a Raman band with a
peak very similar to that of species F.
Helz et al.24 provided a list of possible thioarsenite species
and their predicted Raman shifts based on ab initio calculations. In principle, it should be possible to employ these
calculated Raman frequencies to assign stoichiometries to each
of the species observed in our study. However, we were unable
to match the calculated frequencies of Helz et al.24 to our
observed Raman shifts and maintain consistency with the
trends of the intensities of the spectral bands as a function of
pH and SS/SAs ratio. We suspect that the main reason is that
we have observed at least some species for which Helz et al.24
do not give calculated Raman shifts. Moreover, considering the
large number of species we have observed and how close their
Raman shifts are, even small uncertainties in the shifts
determined from ab initio calculations make it difficult to
make definite assignments of species stoichiometry.
Geochem. Trans., 2002, 3(4) 31-39
As(V) vs. As(III)
In the above discussion, it has been assumed that the
thioarsenic species present in our experiments contain arsenic
in the As(III) oxidation state. Mikenda et al.37 report Raman
bands for the As(V) species AsS432 in the solid salt Na3AsS4?
8H2O and the band attributed to the symmetrical stretch of this
species had a maximum at 385 cm21, which is very similar to
the peak position for the band attributed in this study to species
A. This raises the possibility that the arsenic in species A, and
possibly other species, may be present in the As(V) oxidation
state rather than the As(III) state as assumed. In a Raman study
of thioantimony species, Wood38 similarly observed that the
main thioantimony species in his solutions had bands with a
maximum at approximately the same wavenumber as the Sb(V)
species SbS432 reported by Mikenda et al.37 Finally, based on
ab initio calculations Tossell39 showed that calculated Raman
stretching frequencies for Sb(III)– and Sb(V)–sulfide complexes
were very similar and do not permit a distinction among these
two oxidation states based on Raman frequencies alone.
However, Wood38 (1989) was able to rule out the presence of a
significant concentration of SbS432 based on the polarization
behavior of the Raman bands. On the other hand, others40,41
have presented EXAFS evidence that aqueous Sb(V)–sulfide
species are produced when the Sb(III)–solid Sb2S3 reacts with
aqueous bisulfide.
In the experiments described here, arsenic is clearly in the
As(III) form at low SS/SAs ratios where Raman spectra
characteristic of arsenite species were obtained. Sulfide-free
solutions containing As(III) were mixed with deoxygenated
bisulfide solutions to produce the solutions at high SS/SAs
where thioarsenic species were observed. If these thioarsenic
species contain As(V), this implies that oxidation of As(III)
occurred upon mixing with the reduced bisulfide solution and
formation of the thioarsenic complexes. The question then
becomes, what is the oxidant in the reaction? It seems unlikely
to be dissolved oxygen, owing to the care taken to exclude this
gas during the preparation of the solution. Moreover, even if
the solutions were saturated with oxygen, this would result in
an O2 concentration of no more than 10 mg kg21, or 3.1 6
1024 mol kg21. The lowest As concentration in any of the
solutions employed was 0.01 mol kg21, so even in a solution
saturated in oxygen, there would be too little oxygen available
to oxidize all the As(III) initial present. Because all solutions
were prepared from degassed water and were handled in an
inert atmosphere, the oxygen content should be much lower
than 10 mg kg21, and any residual oxygen left should be
scavenged by the bisulfide in the solutions with high SS/SAs
ratios.
In order to explain the oxidation of Sb(III) to Sb(V) in their
study, Mosselmans et al.40 proposed the following reaction:
Moreover, recently Jayanetti et al.42 have shown that
oxidation–reduction reactions can occur during EXAFS
measurements owing to the radiolysis of water by intense
synchrotron X-ray beams to produce H2 and O2. There is
therefore the possibility that in the EXAFS studies,40,41
oxidation of Sb(III) to Sb(V) occurred during measurements
as a result of radiolysis. It seems only fair to mention that
oxidation–reduction reactions could also be triggered by
intense photon beams such as those employed in laser
Raman spectroscopy. The resolution of the oxidation state
of As and Sb in thio-complexes may require techniques that do
not involve intense beams of electromagnetic radiation.
Implications
Although we can not identify unambiguously the stoichiometries of the thioarsenite species present in our experiments, our
results do have some important implications. Our study clearly
demonstrates the complexity of the As–S–O–H system in that a
relatively large number of species may be present. We have
definitive proof of the existence of at least 6 or 7 different
thioarsenic species in addition to the various arsenite species,
and there may well be more species present that we could not
resolve owing to peak overlap. Although detailed band fitting
may change the details of our interpretations, our data clearly
show the presence of a relatively large number of thioarsenite
species. It may be this degree of complexity which explains the
discrepancies in interpretations of previous studies. Our study
also has implications for EXAFS investigations of such
systems. EXAFS is a very powerful technique for the study
of species in aqueous solution, but it provides a measure of the
average coordination of an element in solution. Where several
species exist simultaneously in significant concentrations, the
interpretation of EXAFS results may not be straightforward.
Thus, it may be necessary that a technique that can ‘‘see’’
individual species, such as Raman spectroscopy, be used in
conjunction with EXAFS. Also, the possibility of radiolytic
oxidation/reduction EXAFS measurements must be taken into
account when making EXAFS measurements on redoxsensitive systems.
Acknowledgement
This research was funded in part by a grant to SAW from
Barrick Goldstrike, administered by the Bureau of Land
Management. SAW also acknowledges receipt of two summer
faculty research fellowships from AWU-DOE (Associated
Western Universities – Department of Energy). CDT and DRJ
were supported by DOE – Basic Energy Sciences, Division of
Engineering and Geosciences. Two Geochemical Transactions
referees are thanked for their helpful comments.
(y 1 1 – x)H1 1 HS2 1 HxSbS3(x 2 3) < HySbS4(y 2 3) 1 H2(g)
References
In this case, H1 is the oxidant. Reaction (2) suggests that,
except in the special case where x ~ 1 1 y, substantial shifts in
pH should be observed as oxidation occurs, unless the solution
is buffered. Thus, if a similar oxidation reaction occurred in our
study of As–sulfide species, we should have seen a strong shift
in pH as oxidation proceeded. During the preparation of
solutions, we did not note any drift in pH, nor did we observe
the effervescence of any gases, which could be attributed to
production of hydrogen. We cannot rule out the possibility that
a pH change occurred in the solution after it was sealed in the
capillary tube, but evolution of hydrogen also was not observed
after the solution was sealed in the tube. Thus, we feel justified
in assuming the presence of As(III)–sulfide species. However,
future investigations of this system should take steps to
determine definitively the oxidation state of As in these
thioarsenic complexes.
1 R. Höltje, Z. Anorg. Chem., 1929, 181, 395.
2 R. Nagakawa, Nippon Kagaku Zasshi, 1971, 92, 154.
3 A. A. Ivakin, S. Vorob’eva, E. M. Gertman and E. M. Voronova,
Russ. J. Inorg. Chem., 1976, 21, 237.
4 G. D. Mironova, A. Zotov and N. I. Gul’ko, Geochem. Int., 1984,
21, 53.
5 C. A. Heinrich and P. J. Eadington, Econ. Geol., 1986, 81, 511.
6 J. M. Ballantyne and J. N. Moore, Geochim. Cosmochim. Acta,
1988, 52, 475.
7 J. Webster, Geochim. Cosmochim. Acta, 1990, 54, 1009.
8 N. N. Akinfiev, A. Zotov and A. Nikonorov, Geochem. Int., 1992,
29, 109.
9 G. Pokrovski, R. Gout, J. Schott, A. Zotov and J.-C. Harrichoury,
Geochim. Cosmochim. Acta, 1996, 60, 737.
10 A. Criaud and C. Fouillac, Geochim. Cosmochim. Acta, 1986, 50,
1573.
11 M. H. Wünschendorff, Bull. Soc. Chim. Fr., 1929, 45, 897.
12 A. K. Babko and G. S. Lisetskaya, Russ. J. Inorg. Chem., 1956, 1,
95.
Geochem. Trans., 2002, 3(4) 31-39
13 M. H. N. Srivastava and S. Ghosh, Proc. Natl. Acad. Sci., India,
1957, 26, section E, part III, 250.
14 M. H. N. Srivastava and S. Ghosh, J. Ind. Chem. Soc., 1958, 35,
1659.
15 J. Angeli and P. Souchay, Compt. Rend. Acad. Sci., Paris, 1960,
250, 713.
16 B. G. Weissberg, Geochim. Cosmochim. Acta, 1966, 30, 815.
17 R. W. J. Raab, PhD Thesis, University of California, Riverside,
1969.
18 S. V. Vorob’eva and A. A. Ivakin, Russ. J. Inorg. Chem., 1977, 22,
1479.
19 A. A. Ivakin, S. Vorob’eva, A. M. Gorelov and E. M. Gertman,
Russ. J. Inorg. Chem., 1979, 24, 1089.
20 G. D. Mironova and A. Zotov, Geochem. Int., 1980, 17, 46.
21 N. F. Spycher and M. H. Reed, Geochim. Cosmochim. Acta, 1989,
53, 2185.
22 G. D. Mironova, A. Zotov and N. I. Gul’ko, Geochem. Int., 1990,
27, 61.
23 L. E. Eary, Geochim. Cosmochim. Acta, 1992, 56, 2267.
24 G. R. Helz, J. A. Tossell, J. M. Charnock, R. A. D. Pattrick,
D. J. Vaughan and C. D. Garner, Geochim. Cosmochim. Acta,
1995, 59, 4591.
25 N. F. Spycher and M. H. Reed, Geochim. Cosmochim. Acta, 1990,
54, 3241.
26 N. F. Spycher and M. H. Reed, Geochim. Cosmochim. Acta, 1990,
54, 3246.
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
R. E. Krupp, Geochim. Cosmochim. Acta, 1990, 54, 3239.
R. E. Krupp, Geochim. Cosmochim. Acta, 1990, 54, 3245.
R. E. Krupp, Geochim. Cosmochim. Acta, 1988, 52, 3005.
N. A. Marley, M. Ott, B. L. Feary, T. M. Benjamin, P. S. Z. Rogers
and J. S. Gaffney, Rev. Sci. Instrum., 1988, 59, 2247.
C. D. Tait, D. R. Janecky and P. S. Z. Rogers, Geochim.
Cosmochim. Acta, 1991, 55, 1253.
M. H. Brooker, G. Hancock, B. C. Rice and J. Shapter, J. Raman
Spectrosc., 1989, 20, 683.
G. E. Walrafen, J. Chem. Phys., 1962, 36, 1035.
B. Meyer, K. Ward, K. Koshlap and L. Peter, Inorg. Chem., 1983,
22, 2345.
T. M. Loehr and R. A. Plane, Inorg. Chem., 1968, 7, 1708.
A. Rogstad, J. Mol. Struct., 1972, 14, 421.
W. Mikenda, H. Steidl and A. Preisinger, J. Raman Spectrosc.,
1982, 12, 217.
S. A. Wood, Geochim. Cosmochim. Acta, 1989, 53, 237.
J. A. Tossell, Geochim. Cosmochim. Acta, 1994, 58, 5093.
J. F. W. Mosselmans, G. R. Helz, R. A. D. Pattrick,
J. M. Charnock and D. J. Vaughan, Appl. Geochem., 2000, 15, 879.
D. M. Sherman, K. V. Ragnarsdottir and E. H. Oelkers, Chem.
Geol., 2000, 167, 161.
S. Jayanetti, R. A. Mayanovic, A. J. Anderson, W. A. Bassett and
I.-M. Chou, J. Chem. Phys., 2001, 115, 954.
Geochem. Trans., 2002, 3(4) 31-39