Cutting together-apart the mould

CUTTING TOGETHER-APART THE MOULD Physarum polycephalum (slime mould/mold) is an organism used in scientific research that is commonly found in piles of leaf litter and human composts. Intelligent yet brainless, P. polycephalum forms a fan-like network of tendrils in its quest for food. Each tendril moves almost visibly by cytoplasmic streaming – a pulsing movement of the liquid inside its cell walls. It is simultaneously beautiful and repulsive – an oozing, bright yellow, lace-like blob. This paper proposes to engage in a (s)mattering of Baradian “agential cuts” to draw out the intra-active performativity of this evocative critter. A dialogue is established between scientific and artistic ontoepistemologies, focusing on how (and why) the matter of slime mould/mold matters. Visual images from explorations which include P. polycephalum are woven through the paper, to support Karen Barad’s assertion that “meaning is not a property of individual words or groups of words but an ongoing performance of the world in its differential intelligibility” [1, p.821]. Author: Tarsh Bates Maddi Boyde Fig.1 Intra-action exhibition detail, 2013. (L-R) Hayden Fowler, New World Order, 2013; Tarsh Bates, in vitero node (Physarum polycephalum), 2013; Kathy High, Mr Fox, 2012 44 i n vitero node (Physarum polycephalum) was an artwork installed as part of the Intra-action: Multispecies becomings in the Anthropocene exhibition at MOP Gallery, Sydney in 2013.[1] The exhibition brought together contemporary artists exploring the relationships between humans and nonhuman animals to elucidate physicist and philosopher Karen Barad’s posthuman theory of intraaction[2] and its insistence on ethical engagements with our world. As this exhibition, including Barad’s theory, is the focus of this special issue and has been discussed in detail by others, this article attends specifically to slime moulds, in particular the species Physarum polycephalum. In 2011, I engaged in a seven month long performance (in vitero) during which I learnt how to care for and think with Physarum polycephalum.[3] As Karen Barad thinks intra-action with slime moulds in her 2012 article Nature’s Queer Performativity, Intraaction exhibition curators Eben Kirksey and Madeleine Boyd invited me to re-present in vitero in 2013 as a material instantiation of Barad’s theorizing. Since for Barad (and for myself as an artist researcher) “theorizing, like experimenting, is a material practice” [2, p.55], this exhibition offered a unique opportunity to entangle Barad’s writing with the performativity of the living in public space. Karen Barad introduces her 2012 article, Nature’s Queer Performativity, with an ethicoonto-epistemological discussion of the intra-activity of thexsocialxslimexmould, Dictyosteliumxdiscoideum. These slime moulds form a single multi-cellular sluglike entity when genetically identical individuals swarm together to form a collective to search for food. Barad’s discussion uses these social amoebae to reinforce her understanding of the inseparability of subjects and objects, and shows that D. discoideum stands-in for a “subterranean” fear that humans have for this inseparability. She unearths moral judgements embedded in a 2009 newspaper report of the discovery of a 40 foot slime mould colony, a “vast and sticky empire,” “oozing” under Houston, Texas [3], which reprises the “creeping threat of communism” that lurks in the 1958 movie The Blob [4, p.26]. Slime moulds are usually considered invisible in discussions of our ethical encounters with nonhumans and slime mould experimentation is not subject to institutional ethics approval. However, as Barad shows in Nature’s Queer Performativity, the amorphous, mindless bodies of slime moulds exemplify fear of consumption of the individual by the Other, epitomised in The Blob [4, p.27]. In this movie, the threat of Communism is metamorphosed into a voracious, unstoppable amoeba-alien: an inhuman blob that uncontrollably accrues mass and moral agency. It follows that slime moulds are both ethically non-existent and morally reprehensible. In the interests of attentiveness and accountability, it is important to disclose that the species of slime mould discussed by Barad in Nature’s Queer Performativity is Dictyostelium discoideum. The species exhibited in Intra-action and discussed here xis xPhysarumx polycephalum. D.xdiscoideum. These are microscopic, social amoeba that swarm to forage, whereas P. polycephalum is not social: it is an individual macroscopic cell with multiple nuclei, much like a human muscle cell or neuron; pulsations in its cytoplasm migrate the cell towards nutrients. P. polycephalum is useful to think intra-action with as it is has gained popularity since 2000 as a model organism for understanding the evolutionary development of intelligence and biocomputational problem solving. My interest lies in how various artistic and scientific explorations of P. polycephalum can be reread as instantiations of Barad’s intra-action. I posit that these explorations are Baradian apparatuses that enact “agential cuts” in order to facilitate understandings of the phenomenon that is “slime mould.” Rather than arguing for or against art or science, I show that they are both useful methods to reveal aspects of the complex intra-actions of humans and slime moulds. Cutting together-apart The “posthumanist” point is not to blur the boundaries between human and nonhuman, not to cross out all distinctions and differences, and not to simply invert humanism, but rather to understand the materializing effects of particular ways of drawing boundaries between “humans” and “non-humans.” –Karen Barad, Nature's Queer Performativity [4, p.31] Following physicist Neils Bohr and the foundations of quantum physics, Barad argues that there is no preexisting, independent reality waiting to be discovered. Rather, the way we understand our world is determined by the apparatuses we use to examine it.[4] For Bohr,–and for Barad,–an apparatus includes both the physical experimental equipment and the theoretical or conceptual framework (including unconscious assumptions) upon which an examination is founded. An apparatus is therefore “onto-epistemological,” an inseparable entanglement of material and knowledge [1, p.816]. In a very broad sense, “science” is one apparatus and “art” is another. Each has materials, practices, histories and 45 \ Tarsh Bates Fig.2 in vitero node (Physarum polycephalum), installation detail, 2013, slime mould, agar, glass © Bates Importantly, phenomena are not just material but as with apparatuses (which are themselves phenomena), inextricably material and discursive [1, p.816]. They are also obviously and fundamentally posthuman, recognising not just the human, but all bodies, where “bodies” includes all matter, not just the living. In Barad’s understanding, matter at all scales has agency in a phenomenon. Bodies obviously exist, but as Barad argues, “what we commonly take to be individual entities are not separate determinately bounded and propertied objects, but rather are (entangled ‘parts of’) phenomena…that extend across (what we commonly take to be separate places and moments in) space and time” [4, p.32]. Since any apparatus is inherently bounded, both physically and conceptually, any examination of our world necessarily includes and excludes. Barad describes this moment of inclusion/exclusion as an “agential cut,” which is not a Cartesian break between subject and object.[8] As has been shown, subjects and objects do not pre-exist an intra-action. An agential cut “enacts a ‘local’ resolution within the phenomenon,” [4, p.32], that is, it focuses on a particular intra-action within a phenomenon, “separating” some components of the phenomenon. Consequently, an agential cut is not an assumptions, some of which are similar and some different. Since the components of any apparatus are inseparable from each other, the “object” of examination is inseparable from the “subject” (or observer). In fact, there can be no object or subject. It is not just that an observation by a pre-existing observer influences and changes a pre-existing observed. The observer and observed are entangled parts of an apparatus which creates a moment of observation.[5] Change any part of the observer or observed, and the moment of observation is necessarily different. Barad, following Bohr, describes the inseparability of subject and object as “intra-action.” Reality then, is constituted by a series/continuum of intra-acting “quantum entanglements,” [6] or “phenomena,” constantly changing, constantly in flux: “phenomena are entanglements of spacetimematter, not in the colloquial sense of a connection or intertwining of individual entities, but rather in the technical sense of—“quantum entanglements,”— which are the (ontological) inseparability of agentially intra-actingx ‘components’” [4,xp.32], i.e.[7] 46 subsequent “materializing effects” produced by these practices: what it “is-means” to be human and/or what it “is-means” to be slime mould [4, p.31].[10] Please note that I am not interested in dichotomising “science” and “art” here, but I am interested in the effects of the differences and similarities of their methodologies. The following discussion attends to the “the particular ways” that specificxexperimentsxwith Physarumxpolycephalum have been conducted by scientists and artists, and what experiences and meanings have been generated from those experiments. I use these experiments, or apparatuses, not as analogies, but as intra-actions, as phenomena, which “force us to confront conditions for the possibility of objectivity, the nature of measurement, the nature of nature and meaning making, and the relationship between discursive practices and the material world” [2, p.24]. observer/subject looking out at an external Other/object. Rather, self looks at self. In Barad’s terms, this separation is an “exteriority-within,” a simultaneous cutting “together-apart” [4, p.32]. An apparatus therefore, “shows” which and how many intra-actions get called a phenomenon, i.e. A time-lapse video taken through a microscope, commonly used in slime mould research, is an example of the material-discursive relationship between apparatus, cut and phenomena. The operator-microscope-camera is the apparatus – images are framed by the microscope and captured by a still camera. A movie camera allows us to view the film. A series of still images are taken, edited and then replayed at 24 frames per second. Each still image is a phenomenon, as is the compiled film. Each image “cuts out” a part of the world, including and excluding, “cutting together-apart.” The editor chooses which parts of the world get cut and which get shown. The film played by the camera shows us a particular selection of the world which would be different if other cuts/images were included. The speed at which the film is replayed (24 frames per second) depicts a particular event time: any faster or slower and we see the world differently. We are unable to see the film without the camera and the technology of the camera determines what we see. Change the technology/apparatus and the film/phenomenon changes. Since phenomena clearly do not always include the human, Barad’s theories offer possibilities for understanding the “practices of differentiating engaged in by nonhumans, whereby nonhumans differentiate themselves from their environments, from other nonhumans, and from humans, as well as from other others” [4, p.31].[9] This, for me, is the posthuman appeal of Barad’s work. Agential cuts are an obligation to pay attention to what gets included and what gets excluded (to what counts) and why, and to the consequences of those inclusions/exclusions. I take up Barad’s exhortation to become accountable for any cuts made [4, p.31]. Barad insists that “any proposal for a new political collective [which surely posthumanism is] must take account of not merely the practices that produce distinctions between the human and the nonhuman, but the practices through which their differential constitution is produced” [2, p.59]. Hence this article explores “the particular ways” the agential cutting enacted by the apparatuses of art and science draw boundaries between “humans” and “non-human” slime moulds. Further, I am interested in the The performativity of mould Images or representations are not snapshots or depictions of what awaits us but rather condensations or traces of multiple practices of engagement. Karen Barad, Meeting the Universe Halfway [2, p.53] Barad’s rejection of representationalism and insistence upon performativity is pivotal to this discussion of the “particular ways” experiences/meanings of slime mould are generated through these apparatuses. Barad is very clear that entities are not independent of their representation. Since there is no subject or object, representationalism, which presupposes a distinction between “individuals and their inherent attributes” and knowledge (or representation) of those individuals, cannot exist in a Baradian world [1, p.804]. The system which assumes: ignores the “practices through which representations are produced.”[11] A Baradian world is not waiting to be represented, it is always already represented, or more correctly, it is always already a representation: a performative representation, where representations are not abstractions of the real, but apparatuses— contiguous, iterative materializations of phenomena. Drawings, photographs, diagrams, language all trace 47 Tarsh Bates Fig.3 in vitero node (Physarum polycephalum) #1, digital print, 2011 © Bates 48 “material engagements with the world” [2, p.49]. It is instead the following system: arm leading to the yolk food source. White areas represent choices of the blank agar arm leading to the oat food source. The x axis shows the different Y-maze arm lengths. Within each arm length, food was either placed at the end of the arm at the start of the experiment (0h) or 2h prior to the start of the experiment. Stars indicate significant preference for 1 arm (binomial test).” Reprinted by permission from Macmillan Publishers Ltd: [Behavioral Ecology] [Reid et al. Amoeboid organism uses extracellular secretions to make smart foraging decisions), copyright (2013). Thinking of a representation as an apparatus enables us to recognise its role in framing knowledge, but more importantly here, forces us to acknowledge its performative materiality. Photographs of slime mould are as much slime mould as Figure 4 or even Figure 5. Importantly however, they connote different understandings of what slime mould is. Cutting together-apart the mould/mold: Nodes and mazes This section discusses the nature of apparatuses through a number of linguistic, artistic and scientific apparatuses that think with P. polycephalum . This section discusses the nature of apparatuses through a number of linguistic, artistic and scientific apparatuses that think with P. polycephalum . Firstly, the linguistic semantics involved in the word “mould,” then a number of scientific experiments, beginning with 2000 maze research conducted by Toshiyuki Nakagaki, Hiroyasu Yamada and Ágota Tóth are examined. Finally, several artistic explorations, including my own, are considered. A Baradian apparatus comprises of three aspects: • • • Conceptual framework, including methodology; Material entanglements; and Material & conceptual outcomes In the rest of this paper, the artistic and scientific apparatuses are introduced and each of the three aspects are thought through in order to understand the resultant phenomena. This discussion shows these apparatuses to be agential, intra-active entanglements of human, slime mould and environment. Fig.4 Experimental setups for agar Y-maze choice experiments, where ES stands for extracellular slime. (a) Self versus conspecific nonself extracellular slime, (b) response to heterospecific extracellular slime, (c) choice between fresh and aged extracellular slime, and (d) patch-mark cues versus food cues behavioral titration.” Reprinted by permission from Macmillan Publishers Ltd: [Behavioral Ecology] [Reid et al. Amoeboid organism uses extracellular secretions to make smart foraging decisions), copyright (2013)]. Apparatus 1: the semantics of mould/mold Before I do this however, I want to make a brief note about the word “mould” itself as an apparatus, which has motivated me to write it here as “mould/mold.”[12] Firstly, the “British” (and Commonwealth) spelling is “mould,” and although its etymology is unclear, it is thought to be derived from the medieval English word “mouled,”[13] meaning “mouldy, rotten or decayed.” “Mould” refers to the “woolly, furry, or staining growth…which forms on food, textiles, etc., esp. in moist warm air,” and has since been adopted into biology to refer to “a fungus, esp. one that produces the abundant visible mycelium or spore mass”. [14] Conversely, the “American” spelling, “mold,” is inherited from the spelling reform that occurred in Fig.5 Choice experiments between an arm containing extracellular slime leading to a yolk food source and an arm of blank agar leading to an oat food source (the experiments in Figure 2d). The proportion of choices made by plasmodia within each treatment group is presented on the y axis. Shaded areas represent choices of the extracellular slime-coated 49 “Mould” has also been used since the 1200s to refer to the action of manipulating matter into “a required shape by pouring or pressing into a hollow form or matrix; to press or cast in or into a particular form.”[16] Although the etymological relationship between “mould” as a manipulation of matter and “mould” as decay is unclear (and not within the scope of this article), slime moulds collapse the definitions into a material-discursive phenomenon: the early 19th century. Donald Scragg found that many American English spellings follow Noah Webster's An American Dictionary of the English Language published in 1828 [7, pp.82–83]. According to Scragg, the change was partly nationalistic. Following Barad’s insistence on attention to “the relationship between discursive practices and the material world,”[15] I am moved to ask about the political motivations supporting this change, given Tarsh Bates Fig.6 in vitero installation detail, 2011 © Bates that the timing around the revolution and the desire to claim a separate identity from Britain. Although these motivations may appear insignificant, or even irrelevant, to a discussion of slime mould/mold, they manifest in confusion around the name in writings about the organism. Allegiances to British or American spelling produce historical and political resonances in any discussion of the organism. As I am writing in a Commonwealth country for a British journal, I adopt the British spelling of “mould.” This one cell is a master shapeshifter. P. polycephalum takes on different appearances depending on where and how it is growing: In the forest it might fatten itself into giant yellow globs or remain as unassuming as a smear of mustard on the underside of a leaf; in the lab, confined to a petri dish, it usually spreads itself thin across the agar, branching like coral [8]. 50 Apparatus 2: The science of mould Apparatus 2.1: Efficient Mapping Apparatus 2.2: Optimal foraging In 2000, a group of scientists, led by Toshiyuki Nakagaki,zannouncedzthat Physarumzpolycephalum exhibited “primitive intelligence” demonstrated during a series of maze-solving experiments [9].[17] The article correlated efficiency with survival, and intelligence with computation. A further experiment, published in 2010 by the Nakagaki group, was even more popular [10]. This experiment, during which slime mould “re-created” a map of the Tokyo rail system, consolidated the popularity and usefulness of “brainless, yet intelligent,” slime moulds as “a biologically inspired mathematical model” [10, p.439]. Slime mould mapping of Iberia, Canada, Australia, Brazil and the UK has since followed, extoling the amazing ability of slime moulds to “efficiently construct transport networks” [11, p.1546].[18] [19] These mapping experiments and the “problem-solving” capabilities of slime mould have established P. polycephalum as a model organism for biocomputing and complex network engineering.[20] Fig.7 “a, Structure of the organism before finding the shortest path. Blue lines indicate the shortest paths between two agar blocks containing nutrients: a1 (4151 mm); a2 (3351 mm); b1 (4451 mm); and b2 (4551 mm). b, Four hours after the setting of the agar blocks (AG), the dead ends of the plasmodium shrink and the pseudopodia explore all possible connections. c, Four hours later, the shortest path has been selected. Plasmodium wet weight, 90510 mg. Yellow, plasmodium; black, ‘walls’ of the maze; scale bar, 1 cm. d, Path selection. Numbers indicate the frequency with which each pathway was selected. ‘None’, no pseudopodia (tubes) were put out.” [9] Maze-solving by Physarum polycephalum. Reprinted by permission from Macmillan Publishers Ltd: [Nature] (Nakagaki et al. Intelligence: Maze-solving by an amoeboid organism), copyright (2000) (doi:10.1038/35035159)) © Toshiyuki Nakagaki 51 Fig.8 Experimental setup. (A) No-choice experiment, diet 1:2, with a total concentration of 40 g·L−1. (B) Choice experiment, diet 6:1 vs. diet 1:2. (C) Multiple-choice experiment. The slime mold was initially placed at the center of the petri dish. © Audrey Dussutour In the wild, slime mould is part of a complex food chain. It grows on decaying organic matter in damp dark environments, consuming bacteria and fungi that in turn feed on the organic matter. It exudes chemicals that break down bacterial and fungal cell walls allowing access to the nutrients within the cells [13, p.121]. In the laboratory, nutrients are usually provided in the form of either rolled oats or glucose. A 2010 research project supplied a slime mould within different ratios of proteins and sugars and showed that slime moulds prefer a food source that comprises two thirds protein and one third carbohydrates [12]. As shown in Fig.8, slime mould was offered various combinations of proteins and carbohydrates. The researchers concluded that the slime mould was able to solve “complex nutritional challenges without possessing a centralized processing center or specialized foraging agents. Plasmodia…were able to alter their growth form and movement to exploit complementary food resources and regulate the supply of carbohydrate and protein to a target ratio that maximized performance” [12, p.4608]. This study was interested in the implications of slime mould foraging and nutritional decision making for soil ecologies. They suggested that “understanding the nutritional currencies that shape exploitation of food resources by organisms such as slime molds and fungi, and the consequences of such nutritional decisions for growth, form, and function…has significance for local and global nutrient cycling and carbon sequestration” [12, p.4608]. Subsequent research found that foraging slime mould avoids areas where it encounters its own slime, increasing its “foraging efficiency” [14, p.812]. The researchers suggested that the slime is a type of “patch marking,” “a reliable and widespread mechanism to ‘memorize’ the location of an unprofitable patch, allowing individuals to recognize previously searched areas and relocate their search to unmarked and unexplored patches” [14, p.812]. The researchers therefore concluded that the slime acts as extracellular spatial “memory,” the first time this has been demonstrated in an organism without a brain or a nervous system [15]. The efficient foraging characteristic of slime mould enables it to negotiate such mazes and transport networks above. However, unconventional computing researcher Andrew Adamatzky recently found that the usual attractants of oats and glucose used in slime mould problem solving experiments were inadequate for highly complex computational problems. Consequently, he conducted research into more complex chemotaxis responses, searching for “novel ways to control propagation of slime mould, in order to program it and make it implement computational operations” [16]. He found that slime Fig.9 The slime mould (yellow blob) was placed at one end of a petri dish with the sugary food source (black smudge) placed at the other end. The U-shaped trap was setup in between [15]. © Chris Reid mould preferred herbal sedatives to oats and glucose, with its strongest preference being for valerian root. “Using these herbal tablets we can attract slime moulds and keep them 'concentrated' in a local domain of space" [Adamatzky cited in 17]. Fig.10 Examples of experiments. (ab) Kalms Sleep (left) vs Kalms Tablets (right): (a) plasmodium propagates to both targets, (b) plasmodium propagates to Kalms Sleep (left); (c) Nytol (left) vs Kalms Tablets (right); (d) Valerian roots (left) vs Nytol (right); (e) Valerian roots (left) vs. Vervain (right); (f) Hops (left) vs. Passion flower (right) [16]. 52 electrode array (shown in Fig.11) which was connected via a computer to the robot. Light detected by sensors on the robot controlled light shone onto the slime mould. The response of the mould to the light was registered by the electrodes, causing a corresponding movement in the legs of the robot. “The robot then scrabbled away from bright lights as a mechanical embodiment of the mould.”[19] The researchers described the process as a transduction of “microphysical scale” environmental/chemical signals by the “intracellular informationxxprocessing”xxsystemxofxxthe Physarum cell, which is then amplified through the interaction loop to the “macroscopic environment” of the robot [18, p.217]. Apparatus 2.3: Emotional cyborgs Fig.12 Cellular robot controller. Sensors on the robot (RB) detect lamp input (LI) and transmit a signal to the computer (PC). The signals from the sensors are recoded by the PC into a spatial light pattern which is projected as light stimulus with a video projector (PR) via mirror (MR) onto the surface of the plasmodium (PP). Oscillations of the plasmodium (PP), which is patterned on an agar plate (AP) by a plastic sheet (PS), are detected by a CCD camera (CC) as intensity changes in light transmitted from the bandpass filtered (BF) light source (LS) [18, p.219]. © Soichiro Tsuda Fig.11 Physarum oscillator circuit. The plasmodium is patterned as six oscillators with star coupling (A)…A diagrammatic view of one branch is shown on the right (B). The oscillator nodes have a diameter of 3 mm; channels are 6 mm × 0.5 mm. A dashed box delineates the area irradiated for light stimulus [18, p.219]. © Soichido Tsuda In 2012, this experiment was extended to control an expressive, female Jules robot made by Hanson Robotics [20]. Here, the slime mould was grown on a 64 electrode array rather than a six node array. Attraction responses were generated by oat flakes and repulsion responses were generated by light. The electrical signals generated by its migration across the array were converted into sounds. The researchers assigned each sound an emotion using the “circumplex model of affect,” which “proposes that all [human] affective states arise from two fundamental neurophysiological systems, one related to valence (a pleasure–displeasure continuum) and the other to arousal, or alertness. Each emotion can be understood as a linear combination of these two dimensions, or as varying degrees of both valence and arousal” [21]. The slime mould researchers aligned the attraction-repulsion responses of the slime mould along the pleasure-displeasure axis of the psychological model and the volume (or strength) Slime mould has been used in attraction and repulsion experiments as it responds rapidly to chemical changes in its environment, and it is photophobic (afraid of light). Its migration towards food sources is an example of an attraction response and its migration away from light is a repulsion response. Commonly used to demonstrate the phenomena of chemotaxis and photophobia to school students,[22] a number of recent sophisticated scientific experiments have explored the biorobotic potential of these phenomena. The first of these developed a control interface between slime mould and a six-legged walking robot in 2006 [18]. Following on from research into Physarum Boolean logic gates, an interaction loop amplified the oscillation of the Physarum in response to light, steering the robot. The slime mould was grown over a six node 53 Fig.13 A graphical representation of the circumplex model of affect with the horizontal axis representing the valence dimension and the vertical axis representing the arousal or activation dimension” [21]; The Gale robot [20] © Jonathan Posner; Sandrine Ceurstemont of the response along the arousal axis. A facial expression corresponding to each emotion was then generated in the Jules robot (Fig.13) . Apparatus 3: Artistic explorations Apparatus 3.1: in vitero (2011-2013) Tarsh Bates Tarsh Bates Fig.14 in vitero, installation detail, PICA, 2011 © Megan in vitero was a durational performance that explored aesthetic experiences of care through prolonged engagement with eight other species of living organisms commonly used in reproductive biology, including P. polycephalum . The performance occurred in two locations: a scientific laboratory at University of Western Australia and The Perth Institute of Contemporary Art. After two and a half months in the laboratory, where I learned to take care of the slime mould, the project moved into the gallery and was open to the public.[23] The mould was installed in the gallery in a customised glass vessel and I lived in the gallery with it for a further three months. During this time, I engaged in necessary and often mundane activities required for the care of the organism and myself. I asked the following questions: How do our behaviours change when we care for other bodies? Is caring for slime mould different in a gallery rather than a laboratory? How do we care for creatures that are not cute or furry? Is it possible to care about something as radically other as slime mould? During the five months of care the slime mould flourished and died several times. I was interested in its ongoing performativity, in its phenomenological engagement with the environments I provided, rather than capturing a single moment of beauty as an insight into its true nature. At times it was dynamic and spectacular, Schlipalius; Physarum polycephalum, installation detail, 2011 © Tarsh Bates moving towards food within hours and forming bright yellow lace networks around the glass vessel. It withdrew to other food, leaving diaphanous traces of itself. One viewer seeing the lace network declared that I had captured it at its most beautiful. Ironically it had run out of food, and was in fact starving rather than flourishing. Another instantiation of the slime mould, in vitero node (Physarum polycephalum ), was included as part of the Intra-action exhibition that inspired this Tarsh Bates Fig.15 in vitero, installation detail, MOP Gallery, 2013 © Tarsh Bates 54 Oliver Kelhammer Fig.16 The IKEA vexation, research stills, 2013. © Oliver Kelhammer special issue of Antennae, 18 months after the in vitero project had finished. A new specimen of P. polycephalum was sourced from a local scientific research laboratory, seeded into the same vessel and placed within the MOP gallery. I considered this installation to be a node of the previous project; a continuation of the exploration of the performativity of slime mould, and an instantiation of Barad’s posthuman intra-action. However, the slime mould refused to perform: sporadic plasmodia sent out in search of food quickly died and no spectacular lace formed to signify an ideal state. Apparatus 3.2: The Ikea vexation (2013) Oliver Kelhammer In The IKEA vexation, Oliver Kelhammer built a model of the floor plan of his local IKEA store, placed an oat in each “department,” and placed the Physarum at the entrance.[24] The floor plan of the IKEA store is both the maze of the scientific experiments and an example of the capitalist architectural phenomenon now known as the Gruen transfer, which is the spatial disorientationxexperiencedxinxshoppingxmalls.[25] Teresa Schubert Fig.17 Somniferous Observatory, 2010, (L-R: untreated, valerian root, cannabis, tobacco) © Teresa Schubert 55 Apparatus 3.5: Physarum Experiment No: 013: The Spelling Test (2010) Heather Barnett This disorientation is designed to encourage unintended purchasing and is due to the intentionally confusing layout of shops and other physical barriers among other environmental cues, such as sound and lighting.[26] Kelhammer “wondered if the slime mould could resist such distractions and efficiently find its way through an IKEA without getting sidetracked?”[27] Apparatus 3.3: Somniferous Observatory (2010) Teresa Schubert Somniferous Observatory is a series of artistic experiments by Teresa Schubert that explored the “self-organisation and pattern formation of Physarum polycephalum under the influence of psychoactive and somniferous substances.”[28] Schubert’s explorations re-visit a series of experiments by pharmacologist Peter Witt, which investigated the influence of various psychoactive drugs on the webs constructed by garden spiders, including caffeine, LSD and amphetamines. The drugs used by Schubert included tobacco, cannabis, St. Johns Wort (an herbal anti-depressant), sertraline hydrochloride (a pharmaceutical anti-depressant), and valerian root (an herbal sedative). The effects of the drugs on the migration and networks formed by the slime mould were documented by Schubert in a series of digital prints. Heather Barnett Fig.19 Physarum Experiment No: 013 – The Spelling Test, 2010. video still © Heather Barnett The Spelling Test is an animation which documents one of a series of artistic experiments conducted by Heather Barnett which explores the “simple yet complex behaviours of this biological and cultural phenomenon”xthatxisxP.xpolycephalum.[30] Directly inspired by the scientific research described above, The Spelling Test explores understandings of intelligence by encouraging the slime mould to form the words “Physarum polycephalum” through nutrient placement. Another work by Barnett, Physarum Experiment No: 019 is an animation which revisits the maze in Nakagaki et al’. 2000 groundbreaking experiment. Apparatus 3.4: Bodymetries (2013) Teresa Schubert Apparatus 3.6: The sublime grotesque: Gail Wight & Phil Ross Gail Wight produced a series of artworks between 1996 and 2009 which focus on the agency of slime mould through representations of cytoplasmic streaming, the mechanism of migration of P. polycephalum. When foraging, the cytoplasm within the cell membrane pulses, moving the cell. Wight’s 1996 work, Slime Trace, creates drawings using the migration of the slime mould towards [31] oats. Creep (2004) and Hydraphilia (2009) are time-lapse videos of cytoplasmic streaming. Creep is a three channel work, whereas Hydraphilia presents nine large monitors, reflecting the nine-headed Hydra of Greek mythology (polycephalum is Latin for “many-headed”). Both works present the slime mould as simultaneously beautiful and grotesque. Similarly, Phil Ross’ 2009 video work Leviathans, focuses on the migration of slime mould.[32] The work has a highly evocative soundscape Teresa Shcubert Fig.18 Bodymetries, 2013. Installation still, Totall Recall, Ars Electronica Festival © Teresa Schubert Bodymetries is a second work by Teresa Schubert that engages with pattern formation by P. physarum.[29] This interactive work is a computer simulation which maps “melanin spots on human bodies to the protoplasmic network of slime mould.” Images of yellow slime mould protoplasm are Projected onto human bodies. Bodymetries presents both slime mould and human skin as biological sensors. 56 Gale Wight Fig. 20 Slime Trace (1996), Creep (2004), Hydraphilia, 2009 © Gale Wight and the beauty of the slime mould is apparent. However, the threat expressed in The Blob and described by Barad weighs heavily in Leviathans, not only because of the reference to the sea monster, Leviathan, of Jewish mythology in the title of the work. Cells collide, merge and engulf, accompanied and enhanced by the dramatic soundscape. The organism presented here is a sublime, monstrous alien. Apparatus 3.7 Being Slime Mould: observation / simulation / enactment (2013) Slimoco Being Slime Mould was a three part artwork during which audiences could view videos of Heather Barnett’s Physarum Experiments, participate in becoming-food for slime mould and become-slime mould.[33] As viewers moved around the exhibition, a computerxxxmodelled xxxPhysarum xxxplasmodium recognised them as food and formed networks between the human “food sources.” A slime mould network was also simulated, where viewers were tied together as a “collective super-organism” and encouraged to forage: to become-slime mould. Apparatus 3.8: Morphs (2013) William Bondin Morphs was a speculative design project which proposed a series of architectural forms which are mobile in response to environmental cues.[34] Inspired by the scientific research described above, William Bondin developed the Morphs to reflect the environmentally stimulated path finding and spatial memory of slime mould. For Bondin, “the interesting thing about slime mould, in particular Physarum polycephalum, is that its cognitive processes occurs within its environment rather than a centralised brain. It is an example of an organism which has developed a clever way of exploiting its surroundings in order to perform navigational tasks and memory-related processes.”[35] The Morphs are potentially communicative, autonomous and heuristic structures that could temporarily form complex structures and perform collective tasks, learning from experience and their environment. Philip Ross Fig. 21 Leviathans, 2009 © Philip Ross 57 Slimoco Fig. 22 Being Slime Mould: observation / simulation / enactment, performance stills, 2013 © Heather Barnett Aspect 1: Conceptual frameworks: Frivolity and utility, control and agency In contrast, the artworks tend to be frivolous explorations of the behaviours of the slime mould. Even those artworks that revisit or use scientific experiments do not frame their outcomes in terms of utility or efficiency, the exceptions being Bondin’s Morphs and Schubert’s Bodymetries. Rather, the inefficiency and performativity of the slime mould is highlighted in playful ways that gently subvert the reduction of slime mould to logic gates. The video works of Gail Wight and Phil Ross and my work in vitero are framed by notions of agency and unknowability rather than optimisation and computation. The slime moulds are allowed to roam, gazed upon as in science experiments, with the same technologies, but the focus is on the body and behaviour of the mould rather than on its function. Understandings of intelligence, memory and The conceptual frameworks for these science and art explorations are very different. With the exception of Christopher Reid’s experiments into foraging, the science experiments adopt an engineering approach which is goal oriented and interested in the utility of P. polycephalum for human purposes, in particular, the solution of complex network problems (transportation) and biocomputing. Consequently, the experiments are framed by engineering concepts of efficiency, optimisation, design and computation. Reid’s experiments are framed by evolution biology and are ecologically focused, however the researchers adopt capitalist concepts of profitability, efficiency and “the costs of ‘lost opportunity,’” [14, p.813], notions prevalent in contemporary evolutionary biology. William Bondin Fig. 23 Morphs, digital image, 2013 © William Bondin 58 can only interact with a virtual slime mould within a restricted projection field. Unlike the scientific experiments however, the artistic experiments are also interested in chance and the agency of the organism. In many explorations, the temporal and sensual aesthetics of viewing/experiencing the slime mould are more important than the visual aesthetics, which opened the experiments to the agency of the mould. Allowing for unpredictability and randomness creates opportunities for complex understandings of the worlding of mould—not just the world that is the “wild,” but the naturecultural world. These artworks position themselves, as Barad says, “not merely to use non/humans as tools to think with, but in thinking with them to face our ethical obligations to them, for they are not merely tools for our use but real living beings” [4, p.33]. By relinquishing some control and opening to the performativity of P. polychepalum these works attempt to bring the human observer into the world of the slime mould, rather than the slime mould into the human world. In attempting to bring the human into the slime mould world, the aesthetics of care are a significant aspect of working with living organisms for both scientists and artists. The aesthetics of care are the visual and phenomenological experiences that occur when a living, non-living or semi-living being is cared for or about. “Caring” in this context constitutes the physical and emotional actions involved in maintaining and sustaining the continued existence of another or attending to the non-existence (or death) of another being/object (it could also apply to the self). These experiences are often contingent upon proximity to the cared for, duration spent caring for, and the power relations between carer and cared for. Attentiveness to the aesthetic experiences of care activities and responses to those activities by the cared for, can disrupt the long standing human/animal dualism and inspire a recognition of our responsibility towards the others with whom we share our world and are often dependent upon. in vitero explicitly explored this aesthetic with respect to P. polychepalum, inherent in the scientific and other artistic experiments described here. emotion frame much of the exploration, artistic and scientific. Mazes have a significant history in our construction of these understandings, beginning with rat experiments in the early 20th century. Decisionmaking within a maze in response to a stimuli is a criteria of intelligence assumed by the science experiments described above. Kelhammer’s The IKEA vexation and Barnett’s The Spelling Test demonstrate the absurdity of using human structures to attribute intelligence to nonhuman organisms. These works question assumptions about intelligence. The slime mould bursts through the walls of Kelhammer’s IKEA maze and, even encouraged by food, is unable to “spell” its name. Likewise, Schubert’s Somniferous Observatory feeds the slime mould with the same drug (Valerian root) as Adamatzky’s scientific experiment, but from curiosity about the effects on the slime mould’s behaviour, not from a desire to improve its intelligence or efficiency. Notions of control and agency are crucial to these explorations. Control is vital to both the methodology and outcomes of scientific experiments. Framed by the lens of the scientific method, P. polychepalum is a pre-existing entity holding information that needs to be discovered. The slime mould is constrained within mazes or on electrodes, and stimulated or repulsed by chemicals or light to achieve particular outcomes. Data generated by these experiments is manipulated and filtered to produce statistical probabilities and predictable, repeatable, useful results. Any deviation is obscured by the statistics or the conclusions. Control is also important in the aesthetic framing of the artworks. The history of Western aesthetics influences understandings of the slime mould as a nonhuman organism and the aesthetic decisions made to produce the artworks. Constraints were placed on the slime mould during all these artworks: physical, such as by mazes or vessels, or by chemicals, light or food in order to elicit responses, such as in Schubert’s Sominferous Observatory and Barnett’s The Spelling Test, and also in my own in vitero, where I occasionally withheld food in order to encourage foraging and migration to coat the glass vessels with yellow lace. Wight and Ross’ videos are highly framed and constructed: Wight’s through the addition of coloured dyes to the substrate and Ross’s through digital colour enhancement and a highly evocative soundtrack. Slimoco’s BeingxxxSlimexxxMould physically constrains both slime moulds and humans. Bodymetries restricts mapping to melanin spots—the slime mould has no choice in its path or its stimuli and the human experience of the work is controlled by technological limitations, as humans Aspect 2: Material entanglements: moulding the mould To maximize its foraging efficiency, and therefore its chances of survival, the plasmodium changes its shape in the maze to form one thick tube covering the shortest distance between the food sources. This remarkable process of cellular computation implies that cellular 59 Tarsh Bates Fig. 24 in vitero, (Physarum polycephalum), 2011 © Tarsh Bates materials can intelligence. show a This is also the case in a number of the artworks: Barnett’s The Spelling Test, Ross’ Leviathans and Wight’s video works. In all these works, the equipment and the environmental context of the slime mould are absent. The opposite is the case in in vitero, Kelhammer’s The IKEA vexation,xxxSchubert’s xxSomniferousxxObservatory, Slimoco’s xxBeingxSlimexMould xxandxxxBondin’s Morphs. In these works, the vessels, food, participants and technology have almost equal importance: the organism is embedded in its context, a Harawayan cyborg. In in vitero, the materials are a substrate for the agency of the slime mould and are as much a part of the aesthetic experience of the human viewer as the living body of the slime mould. The glass vessel references scientific experimentation, and the Victorian-style table evokes that intensive period of exotic specimen collection, with all its colonising connotations. The living body of the slime mould is encountered in all its phases, including death. The human viewer is forced to bend to see the slime mould in the vessel, forcing awareness of their own body in relation to that of the slime mould. They are able to see their own reflection in the glass of the vessel, entangling with the body of the slime mould. Two still images of the original in vitero project were presented above the living installation in the Intraaction exhibition, both entanglements of slime mould and human – my gloved fingers or my writing, its body and food. Both these images show the slime mould at different stages of migration, in moments of primitive –Nakagaki et al. Intelligence: Maze-solving by an amoeboid organism [9] One of the primary considerations in working with living organisms is that these organisms have bodies. In the case of P. polycephalum, the unique materiality of its body is fundamental to both the scientific experiments and the artworks. As Whiting et al. state, “slime mould is capable of optimising the shape of its protoplasmic networks in spatial configurations of attractants and repellents” [23, p.844]. Consequently, the body of P. polycephalum is central to all the explorations described here. However, the body of the slime mould is not a pre-formed truth to be discovered. Each exploration, scientific or artistic, creates unique material conditions in which the body forms and reforms in response to physical, chemical or light obstacles: a collection of “agential cuts” performing the phenomena of slime mould. The materials used in each apparatus are quite similar: scientific glassware, agar, oats, a table, lighting, camera, still or video, computer or other electronic equipment, slime mould plasmodia, the human experimenter, and the human viewer. The focus on function within the scientific experiments relegates all these materials, except for the slime mould plasmodia, (and possibly the maze), to irrelevant components of an objective experiment. 60 living slime mould in in vitero node (Physarum polycephalum): human artefacts overrun by the sublime, grotesque plasmodia. However, the threat from the living organism is allayed in both in vitero andxxSchubert’s xxSomniferousxxObservatory through containment: the slime mould cannot escape the vessels. Any threat in the mobile and autonomous The threat is suppressed in the scientific escape from human constraints. Despite this seemingly utopic relationship however, the slime mould has nowhere to hide. Exposed through glass and to hot spotlights, the slime mould is inescapably on display. The complicated network of “mess mates” present in experimental set-ups is ignored by all the explorations described here. Donna Haraway insists on the consideration of “mess mates” as intrinsic to natureculture: architectural forms of Morphs and in the mapping of human skin by the projected P. polycephalumxxx plasmodiaxxin xxBodymetries xis assuaged by the presence of technology. The threat is suppressed in the scientific experiments by the rhetoric of optimization and rfficiency: The basic story is simple: ever more complex life forms are the continual result of ever more intricate and multidirectional acts of association of and with other life forms. Trying to make a living, critters eat critters but can only partly digest one another. Quite a lot of indigestion, not to mention excretion, is the natural result, some of which is the vehicle for new sorts of complex patternings of ones and manys in entangled association…Organisms are ecosystems of genomes, consortia, communities, partly digested dinners, mortal boundary formations…Eating one another and developing indigestion are only one kind of transformative merger practice; living critters form consortia in a baroque medley of interand intra-actions…Cum panis, mess mates [24, pp.31-32]. slime molds first engulfed the entirety of the edible maps. Within a matter of days…the protists thinned themselves away, leaving behind interconnected branches of slime that linked the pieces of food…the single-celled brainless amoebae did not grow living branches between pieces of food in a random manner; rather, they behaved like a team of human engineers, growing the most efficient networks possible [8]. However, the physical apparatuses that manipulate the bodies of the P. polycephalum —the mazes, food, chemicals and light—are the primary mechanisms of control: “by forcing the slime mould into a U-shaped obstacle, which we placed between the creature and a sugary food source it was drawn to, we found the slime mould used its spatial memory system to help navigate its way out of the obstacle to reach the food” [15]. As described by scientist Chris Reid here, the physical constraints of the maze and the “U-shaped obstacle” literally mould the bodies of the P. polycephalum into two-dimensional configurations. This two-dimensionality is reinforced by photographs and diagrams of the experiments which, without exception, present aerial views of the slime mould. This is also the case with the artworks, with the exception of in vitero , Somniferous Observatory , and Morphs . Adamatzky’s sedative experiments attempt to find more subtle ways of moulding the mould’s body, to improve experimental efficiency. In addition, understandings of slime mould are constrained by the presentation of experimental data as objective representations of truth, despite the obvious inclusion of symbols and overlayed diagrams. In Fig.7 from the Nakagaki maze experiment, for example, the equivalent size and placement of the statistical path Although many of the explorations discussed in this paper allude to supplying oats for the slime mould, usually as an attractant or stimulant, the slime mould does not, in fact, eat the oat. Rather, it eats the bacteria and fungi invisible to the human eye that are actually eating the oat. All these experiments rely on the migration of the slime mould towards the oat/bacteria/fungi mess mates, but the bacteria/fungi are never mentioned. The slime mould must receive a chemical signal from the oat/bacteria/fungi in order to recognise their presence, a type of call-response. Do the oat/bacteria/fungi exude this chemical in order to seduce the slime mould? Is it possible that they “want” to be eaten? The radical strangeness of the slime mould and the threat of consumption and moral repugnance described by Barad manifests in Wight’s work, Creep and Hydraphilia, andxRoss’xLeviathan. Even the titles of these works evoke this fear, but it is most evident in the immense and multiple, uncontrolled and pulsating bodies, the dramatic use of colour, and the soundscape of Leviathans. The threat is also present in the images exhibited with the 61 Tarsh Bates Fig. 25 in vitero, (Physarum polycephalum), 2011 © Tarsh Bates selection diagram visually displaces the bodies of the slime mould into numbers. Similar constraints are also present in several of the artworks, including The IKEA vexation, SomniferousxxObservatory xandxxBarnett’s Physarum Experiments (in particular, No: 019 The Maze). None however, are particularly interested in efficiency, and allow for the agency of the slime mould to create unrepeatable and irrepressible performative encounters. The mould bursts through the maze walls of The IKEA vexation and migrates under the walls in Physarum Experiment No: 019. Interestingly, it does not climb the walls as it does in the vessels of in vitero. No maze obstructs the slime mould’s migration within these vessel: it is free to roam, and moulds itself into three-dimensional forms, defying gravity. Kelhammer describes the performative agency of the slime mould during The IKEA vexation: Physarum tends to behave in what is called a ‘ballistic’ manner; once they have sensed a stimulus they will try to flow toward it taking the most direct route possible. Except when they don't. Because unlike a non-sentient being, slime mould will often abort its migration toward an attractant, if it senses a rival has gotten there first. Sometimes though, competitors will merge into one happy super organism and share the [sic] love and the bounty. Sometimes even that doesn't happen and a slime mould gets distracted and decides to loiter around the perimeter of the petri dish, boycotting the experiment altogether, despite the presence of tasty oatmeal. Who knows why? Are they following their bliss? Or are they just confused?[37] 62 Tarsh Bates Fig. 26 in vitero, (Physarum polycephalum), 2011 © Tarsh Bates In fact, left to its own devices, P. polycephalum forages by sending out “step leaders,” as seen in the image above.[37] The yellow lace-like plasmodia pulse outward in waves in several directions, sending out chemical signals to identify food. The food “responds…with [out]ward signals of its own” [4, p.35]. This is not an isolated identification of the shortest path to food, but a multiplex of communication. Reid et al. showed that these diaphanous tendrils are in fact the embodied, extracellular memory of the mould, necessary for foraging [14]. Is this efficient? Certainly it is a complex form of intelligence. Although many of the experiments reduce the complex behaviour of the mould to Boolean logic gates, the two robotic experiments complicate the notions of body, intelligence, emotion, efficiency and agency. The body of the slime mould is collapsed onto/into the bodies of the computer and those of the robots. The insect-like body of the Zauner bot is described as “scrabbling away from bright lights as a mechanical embodiment of the mould” [19]. The robot-mould unit is agential, autonomous and inhuman. The Gale robot forces us to question how emotions are expressed and who expresses them. The behaviour of the slime mould, which is reduced to Boolean logic gates by others, is transformed through electricity, sound, a complex psychological model of human emotion, back to electrical signals, and into robotic facial expressions. This transformation passes through at least three stages of absurdity: firstly, the assignation of human emotion to slime mould is a surreal notion; secondly, the synaesthesia manifest in the transference of the movement of the slime mould’s body into sound; and finally, the circumplex model of affect was designed to avoid problems experienced in experimental psychology when attributing an emotion to particular facial expression [21]. As Posner et al. describe, Affective behaviors, however, are neither sufficient nor necessary to characterize emotional states (Kagan, 2003; 63 of “slime mould,” a multiplicity of cutting togetherapart. Through the phenomena formed by these apparatuses we perceive some of the performative intra-activity of Physarum polycephalum. Inherent in Barad’s agential realism is a disturbing moral imperative to “face our ethical obligations to [nonhumans], for they are not merely tools for our use but real living beings” [4, p.33]. If, as Gale’s slime mould-robot suggests, slime mould does experience fear (the Zauner bot is also described as being “moved by a slime mould's fears,” [19]), we are forced to rethink our ethical obligations to this radically alien creature. Our current systems are undone. This article has attempted to face Barad’s disturbing imperative, “to stay with the trouble.” It is a humble gesture of an attention to “the materializing effects of particular ways of drawing boundaries between ‘humans’ and [slime moulds]” [4, p.31]. While our ethical obligations to horses, rabbits, mice, or even rats, may be clear (although this is by no means given), what are our obligations to slime mould? Does the matter of slime mould matter? Panksepp, 1998). An animal, therefore, could experience feelings without demonstrating any overt changes in behavior, and conversely, through experimental manipulation, could display affective behaviors without any associated feeling. As Damasio (2003) notes, paramecium will flee from predators, but to argue that singlecelled organisms experience fear makes little sense [21, p.2]. References [1] Barad, Karen. Posthumanist performativity: Toward an understanding of how matter comes to matter. Signs, 2003, 28 (3), pp.801-831. [2] Barad, Karen. Meeting the universe halfway: Quantum physics and the entanglement of matter and meaning. Durham & London: Duke University Press, 2007. [3] Yoon, Carol Kaesuk. Oozing through texas soil, a team of amoebas billions strong. The New York Times, 23 March 2009, [Accessed 24/2/14], Available from: http://www.nytimes.com/2009/03/24/ science/24amoe.html ?_r=2&. [4] Barad, Karen. Nature's queer performativity. Kvinder Køn & Forskning, 2012, 1-2 , pp.25-53. [5] Hird, Myra J. Indifferent globality. Theory, Culture & Society, 2010, 27 (2-3), pp.54-72. Klaus-Peter Zauner Fig.27 The slime mould - pictured on the screen in the [6] Haraway, Donna. Simians, cyborgs and women: The reinvention of nature. New York: Routledge, 1991. experimental set-up - causes a robot to naturally avoid the light”, 2006 © Klaus-Peter Zauner [7] Scragg, Donald. A history of english spelling. Manchester: Manchester University Press, 1974. [8] Jabr, Ferris. "How brainless slime molds redefine intelligence." Scientific American, November 7 2012. Aspect 3: Material-discursive understandings: intelligence, complexity and moral imperatives [9] Nakagaki, Toshiyuki, Hiroyasu Yamada, and Ágota Tóth. Intelligence: Maze-solving by an amoeboid organism. Nature, 2000, 407, p.470. The apparatuses discussed here facilitate access to aspects of the queer critter that is slime mould. None are “correct,” each contributes to our understanding [10] Tero, Atsushi, Seiji Takagi, Tetsu Saigusa, Kentaro Ito, Dan P. Bebber, Mark D. Fricker, Kenji Yumiki, Ryo Kobayashi, and 64 Toshiyuki Nakagaki. Rules for biologically inspired adaptive network design. Science, 2010, 327 (5964), pp.439-442. Notes [11] Reid, Chris R., and Madeleine Beekman. Solving the towers of Hanoi – how an amoeboid organism efficiently constructs transport networks. The Journal of Experimental Biology, 2013, 216 (9), pp.1546-1551. [1] http://intraactionart.com/ [2] “It is through specific agential intra-actions [in contrast to the usual ‘interaction,’ which presumes the prior existence of independent entities/relata] that the boundaries and properties of the ‘components’ of phenomena become determinate and that particular embodied concepts become meaningful. A specific intra-action (involving a specific material configuration of the ‘apparatus of observation’) enacts an agential cut (in contrast to the Cartesian cut—an inherent distinction—between subject and object) effecting a separation between ‘subject’ and ‘object’…relata do not preexist relations; rather, relatawithinphenomena emerge through specific intra-actions” [1, p.815]. [12] Dussutour, Audrey, T. Dussutour, M. Latty, and S. J. Beekman. Amoeboid organism solves complex nutritional challenges. Proceedings of the National Academy of Sciences of the United States of America, 2010, 107 (10), pp.4607-4611. [13] Lloyd, Sarah. Slime moulds: An exquisite obsession. PAN: Philosophy Activism Nature, 2013, 10, pp.119-129. [14] Reid, Chris R., Madeleine Beekman, Tanya Latty, and Audrey Dussutour. Amoeboid organism uses extracellular secretions to make smart foraging decisions. Behavioral Ecology, 2013, 24 (4), pp.812-818. [3] Along with seven other organisms. in vitero was developed at SymbioticA, an art-science research laboratory in the School of Anatomy, Physiology and Human Biology at the University of Western Australia and Perth Institute of Contemporary Art. This project investigated the aesthetics of care, which are the aesthetic experiences which develop through long term encounters with other organisms. [15] Reid, Chris. "The brainless slime mould that remembers where it’s been." The Conversation, 6 October 2012. [16] Adamatzky, Andrew. On attraction of slime mould Physarum polycephalum to plants with sedative properties. Nature Preceedings, 2011. [4] By examination, I mean any process by which we attempt to understand or make our way through the natureculture of our world (consciously or not). [17] Palmer, Jason. Slime mould prefers sedatives, say researchers. BBC News, 10 June 2011, [Available from: http://www.bbc.co.uk/news/scienceenvironment-13683377. [5] By observation, I don’t just mean the visual outcome of a conscious experiment, but any material or conceptual experience. [6] Quantum is defined as “the minimum amount of a physical quantity which can exist” (“quantum, n. 5,” OED online, http://www.oed.com/view/Entry/155941) [18] Tsuda, Soichiro, Klaus-Peter Zauner, and Yukio-Pegio Gunji. Robot control with biological cells. Biosystems, 2007, 87 (2–3), pp.215-223. [7] The following equations are my attempt to understand the relationships between phenomena, intra-actions, apparatuses and agential cuts. [19] Knight, Will. "Robot moved by a slime mould's fears." New Scientist, 13 February 2006. [8] In fact, for Barad, every intra-action is a moment of inclusion/exclusion (refer to Meeting the Universe Halfway for an in-depth discussion [2]). [20] Biever, Celeste. "Robot face lets slime mould show its emotional side." New Scientist, 8 August 2013. [21] Posner, Jonathan, James A. Russell, and Bradley S. Peterson. The circumplex model of affect: An integrative approach to affective neuroscience, cognitive development, and psychopathology. Dev. Psychopathol. 2005, 17 (3), pp.715-734. [9] Myra Hird argues that, in fact, most phenomena do not include the human [5]. It should be noted that Hird does not use the term phenomena. She persists in the notion of “actants,” which Barad categorically rejects as maintaining a subject/object distinction. As this distinction cannot exist within Barad’s universe. Hird does suggest that “actants” exist in “biophysical and biosocial entanglements,” which is definitely a Baradian notion [5, p.1]. Barad however, refers to the entanglement of matter-discourse at a quantum level, which Hird does not. Rather, Hird hinges entanglements on bacteria. [22] Whybrow, Nicolas. Encountering the city: On 'not taking yourself with you'. In Godela Weiss-Sussex and Franco Bianchini, eds. Urban mindscapes of Europe, 97-109. Amsterdam & New York: Rodopi, 2006, pp.97-109. [23] Whiting, James G. H., Ben P. J. de Lacy Costello, and Andrew Adamatzky. Towards slime mould chemical sensor: Mapping chemical inputs onto electrical potential dynamics of physarum polycephalum. Sensors and Actuators B: Chemical, 2014, 191, pp.844-853. [24] Haraway, Donna. When species meet. University of Minneapolis Press, 2008. [10] I use the neologism “is-means” here since material and knowledge are inseparably entangled (see Barad’s “materialdiscursive” [2] and Donna Haraway’s “material-semiotic” [6]). [11] These diagrams are my re-interpretations. [12] OED (http://www.oed.com/view/Entry/122811) Minneapolis: [13] OED (http://www.oed.com/view/Entry/122808) [14] OED (http://www.oed.com/view/Entry/122808) 65 [15] I am well aware of the post-structuralist and feminist legacy here, but others, including Barad herself, eloquently discuss this elsewhere. [35] http://we-make-money-notart.com/archives/2013/10/morphs-mobile-reconfigurablep.php#.U0vHz1eEf7J [16] OED (http://www.oed.com/view/Entry/122813) [36] http://www.oliverk.org/art-projects/research/slime-moldoracle [17] Research had occurred before this, but this article in particular caught popular attention, possibly due to recent interest in biological computing. [37] For a discussion of the role of “step leaders” in the formation of lightning see Barad [4], who takes up Vicky Kirby’s discussion. [18] This subsequent research has been conducted by Andrew Adamatzky’s group in the Department of Unconventional Computing at the University of West England, Bristol. [19] http://www.youtube.com/watch?v=uifP0FFmDyo] [20] Refer to research conducted at the Department of Unconventional Computing at the University of West England, Bristol, the Research Institute for Electronic Science, Hokkaido University, and the Behaviour and Genetics of Social Insects Laboratory, University of Sydney [21] See Supplementary Information [9] for an animated version of a–c . [22] http://www.southernbiological.com/specimens/livingspecimens-and-supplies/plants-and-physarum/l2-30-physarumslime-mouldlive/; http://file.southernbiological.com/Assets/Products/Specime ns/Living_Specimens_and_Supplies/Plants_and_Fungi/L2_30Physarum_slime_mould_culture/CarolinaTipsPhysarum.pdf [23] I also learned how to care for the other organisms, but discussion of the care of these others is undertaken elsewhere. [24] http://www.oliverk.org/art-projects/research/slime-moldoracle [25] Although according to Nicolas Whybrow, this was not the intention of Victor Gruen, the architect to whom this phenomenon is attributed [22, p.97]. [26] http://www.abc.net.au/tv/gruentransfer/faq.htm [27] http://www.oliverk.org/art-projects/research/slime-moldoracle [28] http://theresaschubert.org/3D-SlimeMold.html Tarsh Bates is an artist/researcher interested in how meanings and experience are created and transferred between materials, bodies, environment and culture. She completed a Master of Science (Biological Arts) in 2012 which explored care behaviours for scientific model organisms. She has worked variously as a pizza delivery driver, a fruit and vegetable stacker, a toilet paper packer, a researcher in compost science and waste management, a honeybee ejaculator, an art gallery invigilator, a raspberry picker, a lecturer/tutor in art/science, art history and gender & technology, an editor, a bookkeeper, a car detailer, and a life drawing model. Tarsh is a candidate for a PhD at SymbioticA, The University of Western Australia, where her research is concerned with the aesthetics of interspecies relationships and the human as a multispecies ecology. She is particularly enamoured withCandida albicans. [29] http://theresaschubert.org/bodymetries.html [30] http://theresaschubert.org/bodymetries.html [31] http://www.stanford.edu/~gailw/artdocs/slimetrace.html [32] http://philross.org/#projects/leviathans/ [33] http://slimoco.ning.com/profiles/blogs/being-slime-mouldobservation-simulation-enactment [34] http://beyondperformance.tumblr.com/ 66