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2009, Development
Pakistan journal of pharmaceutical sciences
Phytochemical Analysis, 2014
Pakistan journal of pharmaceutical sciences, 2015
In the present study an analytical method of high-performance thin-layer chromatography (HPTLC) has been developed for quantification of glycyrrhizin for marketed antistressliquorice root capsules (LRC) and herbal tea (HT). Chromatography was performed by using mobile phase ethyl acetate (EA): glacial acetic acid (GAA): Methanol (MeOH): water (H2O) in proportion of 6:2:2:1, v/v/v/v. The developed plate was scanned and quantified densitometrically at absorption maxima 254nm. The method was validated for various analytical parameters viz. precision, accuracy, recovery, robustness, specificity, detection and quantification limits. The developed system was found to give compact spot for glycyrrhizin (Rf= 0.33± 0.001). The linearity relationship was described by the equation Y=6.841X+ 70.428. The limit of detection (34 ng band(-1)), limit of quantification (101ng band(-1)), recovery (99.4-99.8%), and precision (<1.84% and <1.62%; intraday and interday, respectively) were found sati...
Asian Journal of Pharmaceutical and Clinical Research, 2021
Objective: The pure form of glycyrrhizin was bought from Yucca Enterprises, Mumbai-37, India. The pure form of drug used in treatment of acne vulgaris disease. Pharmacognostical studies of Glycyrrhiza glabra L. indicated surprising antibacterial action against Propionibacterium acnes. Methods: A simple, rapid, accurate, precise, and economic spectrophotometric technique for estimation of glycyrrhizin in methanolic extract of G. glabra L. have been developed. Glycyrrhizin exhibit absorbance most at 254 nm when phosphate buffer (pH-6.8) methanol is used as solvent in 70:30 proportion, so absorbance was once measured at the identical wave lengths for the determination of glycyrrhizin. Glycyrrhizin obeys the Beer Lambert’s law in the concentration range of 4–24 μg/ml. Results: This method was validated according to International Council for Harmonization guidelines and can be adopted for the general analysis of glycyrrhizin in hydroalcoholic extract of G. glabra. The approach is simple,...
Objective: To develop a simple sensitive high performance thin layer chromatography densitometric method for quantification of glycyrrhizin in the Glycyrrhiza glabra root m(HePthTLaCn)o-l extract and licorice root capsule methanol extract. Methods: Chromatography was performed on glass-backed silica gel 60 F254 HPTLC plates with vth/ve agsre meno bsiollev epnhtass eet.h Sycl aancneitnagte :a nglda cqiuaal natciefitcica taiocnid o: fm deetvhealnoople: dw aptleart ei nw parso dpoonrteio dne onfs i6t:o2m:1:e0t.r5i,c va/lvly/ aatn d25 r4o bnums.t nTehses macectohroddi nwga tso vInatleidrnataetdio nfoarl dCeotnefcetrieonnc aen odn qHuaarnmtiofniciazatitoionn l igmuiitdse, lpinreesc.i sion, recovery Results: The system gave compact spot for glycyrrhizin (Rf=0.280依0.001). The regression curve qofu astnatnifdicaardti owna s(4 7fo.1u nndg tpoe br eb aYn=d4).,2 1r3exc+o2v2e.r0y7 8(.9 9T.h4e limit of detection (15.7 ng per band), limit of %-99.8%) and precision (≤1.62% and ≤1.84%; intraday and...
Jpc – Journal Of Planar Chromatography – Modern Tlc, 2018
Chromatographia, 2009
Glycyrrhiza glabra Linn., commonly known as liquorice, is a reputed drug of Ayurveda. In the present work we developed and validated densitometric methods for quantification of glycyrrhizin, glycyrrhetinic acid, apigenin, kaempferol and quercetin using HPTLC. The developed methods were found to be precise and accurate. The amount of glycyrrhizin, glycyrrhetinic acid, and quercetin was found to be 1.070, 0.84 and 0.271% w/w, respectively. Apigenin and kaempferol were quantified in free (0.007 and 0.033% w/w) as well as bound (0.021 and 0.074% w/w) forms. This is the first report of simultaneous quantification of glycyrrhetinic acid and apigenin as well as kaempferol and quercetin from G. glabra. Furthermore, no TLC densitometric methods have been reported for the quantification of apigenin, kaempferol and quercetin from G. glabra.
Drug resistance in tumor cells is a significant roadblock in the clinical management of advanced or recurrent diseases in endometrial cancers. As a part of the tumor-stromal ecosystem, tumor cells are ecologically connected to the cancer-associated fibroblasts (CAFs), which form contributory elements of the tumor microenvironment (TME). We recently reported a novel model of patient-derived CAF-based 2-cell Hybrid Co-Culture (HyCC) to evaluate CAFs' role in developing drug resistance and understanding personalized tumor cell-CAF dialogue. Using our 2-cell HyCC model of patient-derived endometrial CAFs, we present data to demonstrate the direct counter-inhibitory effect of CAFs to combinations of cytotoxic and targeted drugs in developing drug resistance. CAFs derived from resected endometrial tumor samples were first passage-wise characterized before and after freeze-thaw for their positive and negative CAF markers expression pattern. Paclitaxel and its combination with copanlisib, TAK228, lenvatinib, and trametinib were used to test the 3D clonogenic growth of endometrial AN3CA cells on endometrial CAFs. We demonstrate that CAF-mediated resistance to antitumor drugs occurs via direct and indirect contact with CAFs in HyCC. Our data established the strength of the 2-cell HyCC model of patient-derived CAFs in solid tumors and provided a model of resistance to antitumor drugs tailored on a patientto-patient basis.
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