Major barley

CULTIVAR DESCRIPTION Major barley W. G. Legge1, J. R. Tucker1, T. G. Fetch, Jr.2, S. Haber2, J. G. Menzies2, J. S. Noll2, A. Tekauz2, T. K. Turkington3, and M. E. Savard4 Can. J. Plant Sci. Downloaded from pubs.aic.ca by Agriculture and Agri-food Canada on 12/06/13 For personal use only. 1 Agriculture and Agri-Food Canada, Brandon Research Centre, P.O. Box 1000A, R.R. #3, 2701 Grand Valley Road, Brandon, Manitoba, Canada R7A 5Y3; 2Agriculture and Agri-Food Canada, Cereal Research Centre, 195 Dafoe Road, Winnipeg, Manitoba, Canada R3T 2M9; 3Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C&E Trail, Lacombe, Alberta, Canada T4L 1W1; and 4Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, 960 Carling Avenue, Ottawa, Ontario, Canada K1A 0C6. Received 1 October 2012, accepted 25 October 2012. Legge, W. G., Tucker, J. R., Fetch, Jr., T. G., Haber, S., Menzies, J. G., Noll, J. S., Tekauz, A., Turkington, T. K. and Savard, M. E. 2013. Major barley. Can. J. Plant Sci. 93: 291
297. Major is a hulled two-row spring malting barley (Hordeum vulgare L.) cultivar widely adapted to western Canada. Developed from the cross Rivers/Newdale made in 1999, Major was evaluated in the Western Cooperative Two-row Barley Registration Test (2006
2007) and the Collaborative Malting Barley Trials (2007
2008) conducted by the malting and brewing industry before being registered in 2009. Major has an excellent combination of agronomic traits and disease resistance with malting quality similar to AC Metcalfe, a cultivar widely used commercially by the malting and brewing industry in domestic and export markets. Key words: Malting barley, Hordeum vulgare L., cultivar description, agronomic traits, disease resistance, malting quality Legge, W. G., Tucker, J. R., Fetch, Jr., T. G., Haber, S., Menzies, J. G., Noll, J. S., Tekauz, A., Turkington, T. K. et Savard, M. E. 2013. L’orge Major. Can. J. Plant Sci. 93: 291
297. Major est un cultivar d’orge brassicole de printemps à deux rangs (Hordeum vulgare L.) très bien acclimaté à l’Ouest canadien. Issu du croisement Rivers/Newdale réalisé en 1999, Major a été évalué dans le cadre des essais coopératifs de l’Ouest sur l’orge à deux rangs (2006
2007) et des essais coopératifs sur l’orge brassicole (2007
2008) entrepris par l’industrie brassicole avant d’être homologué en 2009. Major se caractérise par une excellente combinaison de facteurs agronomiques et de résistance à la maladie, et sa qualité brassicole est similaire à celle d’AC Metcalfe, une variété très employée commercialement par l’industrie brassicole sur les marchés canadiens et d’exportation. Mots clés: Orge brassicole, Hordeum vulgare L., description de cultivar, paramètres agronomiques, résistance à la maladie, qualité brassicole Major is a hulled two-row spring malting barley (Hordeum vulgare L.) cultivar developed at the Agriculture and Agri-Food Canada (AAFC) Brandon Research Centre, Brandon, MB. It received registration No. 6604 from the Variety Registration Office, Plant Products Division, Canadian Food Inspection Agency (CFIA) on 2009 May 25. Plant Breeders’ Rights for Major (certificate No. 4214) were granted by the Plant Breeders’ Rights Office, CFIA on 2011 Nov. 17. Pedigree and Breeding Methods Major was developed from the cross Rivers/Newdale made in 1999 in the greenhouse at the AAFC Brandon Research Centre, Brandon, MB. Rivers is a two-row feed barley cultivar developed at AAFC Brandon with good agronomic performance and disease resistance. Newdale is a two-row malting barley cultivar that showed a significant advance in grain yield when it Can. J. Plant Sci. (2013) 93: 291
297 doi:10.4141/CJPS2012-248 was in the registration tests (Legge et al. 2008). It has occupied about 5% of the two-row malting barley acreage in western Canada from 2007 to 2011, and has been used commercially by one malting company. Early generations were handled by a modified bulk method. The F1 generation was grown as a bulk in the greenhouse, and F2 as a bulk plot in the field at Brandon in 2000. The F3 generation was grown as a bulk increase in the 2000
2001 winter nursery at Southern Seeds Technology, Leeston, New Zealand. The F4 generation grown in two bulk plots at Brandon in 2001 was lost due to early, severe lodging, and had to be repeated as a bulk increase in the 2001
2002 winter nursery at Southern Seeds Technology, Leeston, New Zealand. About 1000 spikes were selected at random from this bulk increase, and threshed individually with harvested seeds from each spike planted as a single F5 progeny row in the field at Brandon in 2002. Progeny rows, one of which 291 Can. J. Plant Sci. Downloaded from pubs.aic.ca by Agriculture and Agri-food Canada on 12/06/13 For personal use only. 292 CANADIAN JOURNAL OF PLANT SCIENCE became BM9929-524, were selected on the basis of height, maturity, lodging resistance, general appearance, and field disease reaction with spot blotch [Cochliobolus sativus (Ito & Kurib.) Drechs. ex Dast.] being the predominant disease. Selected F6 lines were grown as single plots in a preliminary yield test with repeated checks at Brandon in 2003. Selection was based on the same criteria plus yield, heading date, kernel plumpness, test weight, kernel weight, and preliminary malting quality analyses (i.e., grain protein concentration, alpha amylase activity, diastatic power, fine grind extract, soluble protein concentration, and ratio of soluble to total protein concentration) conducted at the AAFC, Cereal Research Centre, Winnipeg, MB. They were also evaluated at Brandon for reaction to spot blotch and netted net blotch (Pyrenophora teres Drechs. f. teres Smedeg.) in the irrigated leaf disease nursery, and to fusarium head blight (FHB) incited by Fusarium graminearum Schwabe on a 0
5 scale based on visual symptoms in the irrigated FHB nursery (Legge et al. 2004). BM9929-524 was advanced to a replicated preliminary yield test at Brandon in 2004 where it was evaluated for the same traits as the previous year plus suitability for straight-cutting, kernel brightness, hull peeling, advanced malting quality analyses (i.e., the same traits as for preliminary analyses plus wort viscosity), and reaction to stem rust (Puccinia graminis Pers.), scald [Rhynchosporium secalis (Oud.) J.J. Davis], spotted net blotch (P. teres Drechs. f. maculata Smedeg.), loose smut [Ustilago nuda (Jens.) Rostr.], covered smut [U. hordei (Pers.) Lagerh.] and false loose smut (U. nigra Tapke.). In 2005, BM9929-524 was grown in an advanced yield test at six locations in western Canada (Brandon, Hamiota and Wawanesa, MB; Saskatoon and Wakaw, SK; and Lacombe, AB) where it was evaluated for the same traits as in 2004 plus common root rot [C. sativus (Ito & Kurib.) Drechs. ex Dast.] reaction, and deoxynivalenol (DON) concentration by harvesting and threshing the row from the FHB nursery at Brandon, grinding a 20-g sample of the grain and sending a 1-g subsample to the Eastern Cereal and Oilseed Research Centre (ECORC), Ottawa, ON, for analysis using the Enzyme-Linked ImmunoSorbent Assay (ELISA) technique. Malting quality was determined on grain from four locations (Hamiota, Saskatoon, Wakaw and Lacombe) for the same traits as in the previous year plus malt beta-glucan concentration. BM9929-524 was advanced in 2006 to the Western Cooperative Two-row Barley Registration Test, where it was evaluated for 2 years as TR06297. TR06297 was also evaluated in the 2007 and 2008 Collaborative Malting Barley Trials conducted at the pilot-scale level by the malting and brewing industry as part of the registration recommending process under the auspices of the Prairie Recommending Committee for Oat and Barley. Performance Major is widely adapted to western Canada, and outyielded the malting check cultivar AC Metcalfe (Legge et al. 2003) by 9% across all soil zones over 2 yr of evaluation in the Western Cooperative Two-row Barley Registration Test (Table 1). In these trials, it was about 4 cm shorter than AC Metcalfe and slightly more resistant to lodging with similar days to heading and maturity (Table 2). Major had higher kernel weight and plumpness, but lower test weight than AC Metcalfe. Thus, Major has a superior combination of agronomic traits when compared with AC Metcalfe over 2 yr, and also over one year with CDC Copeland which was included as a new malting check cultivar in 2007. Overall, Major was 5% lower yielding than the feed check cultivar Xena. During its 2 yr in Western Cooperative Two-row Barley Registration Test, Major had a malting quality profile generally similar to AC Metcalfe, which is widely used commercially in domestic and export markets (Table 3). Minor differences in the malting traits of Major relative to AC Metcalfe were noted during the 2 yr of pilot-scale testing by industry Table 1. Grain yield (kg ha 1) for Major and check cultivars, Western Cooperative Two-row Barley Registration Test, 2006 and 2007 Soil zone Black (east)z Browny Black & Grey (west)x Overall Malting Feed Malting Malting Malting 5516 6784 5942 5735 6824 670 5 5188 6423 5400 5460 6130 616 7 4286 5345 4458 4435 4988 538 4 5065 6266 5334 5290 6061 336 16 Malting Feed Malting Malting 5508 5549 5187 5711 364 4 4780 5659 5214 5260 464 6 5109 5656 4807 4951 633 5 5084 5629 5071 5277 302 15 Overall means (2006
2007) Xena Feed 6235 AC Metcalfe Malting 5607 Major Malting 6329 384 LSDw0.05 No. of tests 9 6071 5314 5728 366 13 5518 4652 4967 425 9 5958 5207 5682 222 31 Cultivar Means for 2006 Harrington Xena AC Metcalfe CDC Kendall Major LSDw0.05 No. of tests Means for 2007 CDC Copeland Xena AC Metcalfe Major LSDw0.05 No. of tests z Class Black Soil Zone (east): Brandon and Glenlea (2006), MB; Indian Head, Melfort, and Regina, SK. y Brown Soil Zone: Beiseker, Lethbridge and Trochu (2006), AB; Harris (2006), Saskatoon, Scott (2007), Swift Current and Watrous, SK. x Black and Grey Soil Zone (west): Beaverlodge, Calmar, Fort Vermilion, and Lacombe, AB; Dawson Creek (2007), BC. w Least significant difference among cultivar means at the 5% probability level, where each test was treated as one replication. LEGGE ET AL. * MAJOR BARLEY 293 Table 2. Agronomic trait data for Major and check cultivars, Western Cooperative Two-row Barley Registration Test, 2006 and 2007 Can. J. Plant Sci. Downloaded from pubs.aic.ca by Agriculture and Agri-food Canada on 12/06/13 For personal use only. Cultivar Days to heading Days to maturity Height (cm) Lodging (1
9)z Test weight (kg hL1) 1000-kernel weight (g) Plump (%)y Means for 2006 Harrington Xena AC Metcalfe CDC Kendall Major LSDx0.05 No. of tests 58.0 57.8 58.4 59.2 57.7 0.8 13w 86.1 87.5 86.9 86.0 86.1 0.9 13v 81.0 83.6 83.1 80.8 80.9 1.7 14u 6.0 4.8 5.7 6.8 4.8 3.4 2t 64.5 67.0 66.2 65.4 65.5 1.0 13s 42.6 49.8 44.7 43.4 46.1 1.4 13r 88.0 93.4 89.9 92.7 91.5 3.5 10q Means for 2007 CDC Copeland Xena AC Metcalfe Major LSDx0.05 No. of tests 59.0 56.6 56.6 57.5 0.8 11w 90.0 90.3 89.2 89.5 1.1 13v 87.0 85.6 85.9 80.6 2.3 14u 4.5 3.8 4.2 3.5 2.6 2t 63.3 66.2 65.2 64.3 0.8 13s 43.8 47.2 42.7 44.2 1.1 12r 88.0 89.0 87.8 88.4 2.5 11q Overall means (2006
2007) Xena 57.2 AC Metcalfe 57.6 Major 57.6 0.6 LSDx0.05 No. of tests 24w 88.9 88.0 87.8 0.8 26v 84.6 84.5 80.8 1.4 28u 4.3 4.9 4.2 1.8 4t 66.6 65.7 64.9 0.6 26s 48.6 43.7 45.2 0.9 25r 91.1 88.8 89.9 2.0 21q z 1 no lodging; 9completely lodged. Kernel plumpness (%) as determined over a 6/64 inch (2.38 mm) slotted screen. Least significant difference among cultivar means at the 5% probability level, where each test was treated as one replication. w Locations: Brandon, MB; Indian Head, Melfort (2006), Regina (2006), Saskatoon, Swift Current and Watrous, SK; Beaverlodge, Beiseker, Calmar, Fort Vermilion, Lacombe (2007), Lethbridge and Trochu (2006), AB. v Locations: Brandon and Glenlea (2006), MB; Indian Head, Melfort (2007), Regina, Swift Current, and Watrous, SK; Beaverlodge, Beiseker, Calmar, Fort Vermilion, Lacombe, Lethbridge and Trochu (2006), AB; Dawson Creek (2007), BC. u Locations: Brandon and Glenlea (2006), MB; Indian Head, Melfort, Regina, Saskatoon, Scott (2007), Swift Current and Watrous, SK; Beiseker, Calmar, Fort Vermilion, Lacombe, Lethbridge and Trochu (2006), AB; Dawson Creek (2007), BC. t Locations: Melfort, SK; Lethbridge, AB. s Locations: Brandon, MB; Harris, Indian Head, Regina, Saskatoon and Watrous, SK; Beaverlodge, Beiseker, Calmar, Fort Vermilion, Lacombe, Lethbridge and Trochu (2006), AB; Dawson Creek (2007), BC. r Locations: Brandon, MB; Harris, Indian Head, Regina, Saskatoon and Watrous, SK; Beaverlodge, Beiseker, Calmar, Fort Vermilion, Lacombe, Lethbridge and Trochu (2006), AB. q Locations: Brandon, MB; Harris, Indian Head, Regina (2007), Saskatoon and Watrous, SK; Beiseker, Calmar, Lacombe, Lethbridge and Trochu (2006), AB; Dawson Creek (2007), BC. y x in the Collaborative Barley Quality Trial (Table 4). When the results are considered across both cooperative tests and collaborative trials, the malting quality of Major was similar to AC Metcalfe overall, except for slightly higher beta-glucan concentration than AC Metcalfe. When compared with CDC Copeland over 1 yr in the cooperative test and 2 yr in the collaborative trials, Major had higher grain, malt and soluble protein concentrations, diastatic power, alpha amylase and beta glucan concentration than CDC Copeland but lower friability. Thus, Major would be better suited to markets that use AC Metcalfe rather than CDC Copeland. Market development is currently underway for Major, so commercial acceptance by the malting and brewing industry is not known at this time. Other Characteristics PLANT. Erect juvenile growth; whitish coleoptile with very thin green strip at tip and medium elongation; medium green leaves with slight waxy bloom, green lower leaf sheath, glabrous sheath and blade; intermediate to upright flag leaf, medium to long length, medium width, white to purplish auricles, glabrous sheath, blade and auricles, and pronounced waxy sheath; medium green stem with slight to pronounced waxy bloom, thin to medium thickness, slight stem exertion, 5 nodes, straight to slightly curved neck, and closed cup with V-shaped collar. SPIKE. Two-row type, slightly tapering to parallel shape, medium density, medium in length, erect to semi-erect attitude, with medium glaucosity; rough lemma awns longer than the spike with green tips, with a few barbs on lateral veins; glumes medium to long in length with medium long to long hairs confined to a band, rough glume awns with green to slightly purplish tips shorter in length than the glumes; first segment of rachis short with weak to medium curvature, rachis edges strongly pubescent; sterile spikelet with weakly divergent attitude; median spikelet with longer glume and awn than grain. 294 CANADIAN JOURNAL OF PLANT SCIENCE Table 3. Malting quality trait dataz for Major and check cultivars, Western Cooperative Two-row Barley Registration Test, 2006 and 2007y Germination energy Malt hull peeling (%)x 99 94 96 98 3 3





10.0 6.7 5.1 4.3 9.9 2 97 98 98 1 3 96 96 89 13 3 46.6 46.9 46.6 1.1 3 5.7 4.8 4.6 8.0 2 99 99 1 6 95 94 9 6 46.9 46.6 2.1 3 5.8 4.5 5.4 4o 1000-kernel weight (g) Grain protein (g hg1)xw 4 ml (%) Means for 2006 Harrington AC Metcalfe CDC Kendall Major LSDp0.05 No. of tests 88.7 91.7 92.9 92.9 4.4 3 43.0 44.8 42.4 45.6 2.2 3 11.5 11.7 11.8 11.3 1.0 3 100 99 99 99 2 3 Means for 2007 CDC Copeland AC Metcalfe Major LSDp0.05 No. of tests 94.1 92.8 95.2 3.0 3 43.1 42.3 44.2 4.7 3 10.2 10.7 11.0 0.9 3 Overall means (2006
2007) AC Metcalfe 92.2 Major 94.0 p 3.4 LSD0.05 No. of tests 6 43.6 44.9 3.0 6 11.2 11.1 0.5 6 Cultivar Can. J. Plant Sci. Downloaded from pubs.aic.ca by Agriculture and Agri-food Canada on 12/06/13 For personal use only. Steep-out Moisture (%)x Kernel plumpness (%)x Cultivar Fine grind extract (g hg 1)xv 8 ml (%) Soluble protein Soluble to total Diastatic Alpha amylase (g hg 1)xv protein (%)xu power (8L)wt (DU)ws Beta-glucan (mg L 1)r Wort viscosity Friability (cps)wq (%)x Means for 2006 Harrington AC Metcalfe CDC Kendall Major LSDp0.05 No. of tests 80.0 80.4 80.0 80.3 0.7 3 4.87 4.85 4.93 4.77 0.32 3 43.1 41.9 41.9 42.3 3.7 3 107 126 145 114 20 3 58.3 61.9 61.0 56.1 7.3 3 111 64 51 74 68 3 1.44 1.42 1.41 1.41 0.02 3 94.1 90.5 94.7 97.1 9.8 2 Means for 2007 CDC Copeland AC Metcalfe Major LSDp0.05 No. of tests 81.1 81.7 81.0 0.7 3 4.62 4.78 4.92 0.20 3 45.6 46.7 46.2 3.8 3 105 123 136 11 3 52.0 68.0 68.0 6.0 3 80 87 116 87 3 1.43 1.42 1.41 0.01 3 98.4 99.4 99.0 3.0 2 Overall means (2006
2007) AC Metcalfe 81.1 Major 80.6 0.6 LSDp0.05 No. of tests 6 4.81 4.85 0.27 6 44.3 44.2 2.1 6 125 125 16 6 64.9 62.0 4.5 6 76 95 38 6 1.42 1.41 0.01 6 94.9 98.0 6.5 4o z Malting quality characteristics determined by industry at the micro-malting level using procedures similar to the Grain Research Laboratory (GRL), Canadian Grain Commission, Winnipeg, MB (Mather et al. 1997). y Locations (company lab): 2006
Beiseker, AB (GRL), Harris, SK (GRL), and Brandon, MB (Anheuser-Busch Inc.); 2007 - Beiseker, AB (GRL), Harris, SK (GRL), and Beaverlodge, AB (Anheuser-Busch Inc.). x Expressed as % by the malting and brewing industries. w On a grain dry matter basis. v On a malt dry matter basis. u Ratio of soluble protein to total protein concentration. t Degrees Lintner. s Dextrinizing unit measure of alpha amylase activity. r On a malt extract basis, expressed as ppm by the malting and brewing industries. q Centipoise, international viscosity units used by the malting and brewing industries. p Least significant difference among cultivar means at the 5% probability level, where each test was treated as one replication. o Data not collected in 2006 for Brandon, MB (Anheuser-Busch Inc.) and in 2007 for Beaverlodge, AB (Anheuser-Busch Inc.); mean of four tests. KERNEL. Covered (hulled), medium length and width, colourless aleurone, short to medium long rachilla with long hairs, a few abnormal rachillas, green lateral lemma nerves, clasping lodicules, no hairs on ventral furrow, and horseshoe depression basal marking. QUALITY. Very good malting quality (Tables 3 and 4). DISEASE REACTION. Resistance to spotted net blotch and loose smut; moderate resistance to covered smut, LEGGE ET AL. * MAJOR BARLEY 295 Table 4. Pilot-scale malting quality trait dataz for Major and check cultivars, Collaborative Malting Barley Trials, 2007 and 2008y Germination energy Cultivar AC Metcalfe CDC Copeland Major LSDo0.05 No. of tests Can. J. Plant Sci. Downloaded from pubs.aic.ca by Agriculture and Agri-food Canada on 12/06/13 For personal use only. Cultivar AC Metcalfe CDC Copeland Major LSDo0.05 No. of tests Cultivar AC Metcalfe CDC Copeland Major LSDo0.05 No. of tests Kernel plumpness (%)x 1000-kernel weight (g) Grain protein (g hg 1)xw 95.7 96.6 95.6 1.5 9 46.8 47.2 46.3 0.9 8n 11.9 11.1 11.8 0.6 9 Malt protein (g hg1)xv 4 mL (%) 95 97 98 3 8n 8 mL (%) 88 92 91 4 8n Steep-out Moisture (%)x Friability (%)x 45.2 45.0 46.2 0.5 9 Fine grind 708C coarse grind Fine-coarse Soluble protein Soluble to total extract (g hg 1)xv extract (g hg1)xv difference (g hg1)xv (g hg1)xv protein (%)xu 11.7 11.2 11.6 0.8 9 80.9 81.3 81.0 1.0 9 79.4 79.7 79.9 1.0 7l 1.3 1.4 1.3 0.8 7l Diastatic power (8L)wt Alpha amylase (DU)ws Beta glucan (mg L 1)r Wort viscosity (cps)wq Barley (%)xp Malt (%)xp 1.52 1.51 1.50 0.05 9 2.1 2.0 3.3 1.3 9 4.9 3.4 5.9 2.0 8k 153 130 158 13 9 73.2 83.2 74.1 6.6 7m 69.0 59.8 72.1 5.4 9 227 182 270 60 9 4.92 4.89 5.10 0.13 9 42.3 44.0 44.1 2.7 9 Peeled & Broken z Malting quality characteristics determined by industry using procedures similar to the Grain Research Laboratory (GRL), Canadian Grain Commission, Winnipeg, MB (Mather et al. 1997). y Locations (company lab): 2007
Beiseker, AB (ADM Malting Canada), Lacombe, AB (Prairie Malt Ltd.), Neapolis, AB (Anheuser-Busch Inc.), Saskatoon, SK (GRL); 2008
Beiseker, AB (GRL), Lacombe, AB (Rahr Malting Co.), Neapolis, AB (Prairie Malt Ltd.), Saskatoon, SK (Malteurop Canada), Watrous, SK (GRL). x Expressed as% by the malting and brewing industries. w On a grain dry matter basis. v On a malt dry matter basis. u Ratio of soluble protein to total protein concentration. t Degrees Lintner. s Dextrinizing unit measure of alpha amylase activity. r On a malt extract basis, expressed as ppm by the malting and brewing industries. q Centipoise, international viscosity units used by the malting and brewing industries. p Percentage of peeled and broken barley and malt, respectively, as measured by industry. o Least significant difference among cultivar means at the 5% probability level, where each test was treated as one replication. n Data not collected in 2008 for Lacombe, AB (Rahr Malting Co.). m Data not collected in 2007 for Neapolis, AB (Anheuser-Busch Inc.) and in 2008 for Lacombe, AB (Rahr Malting Co.). l Data not collected in 2007 for Lacombe, AB (Prairie Malt Ltd.) and in 2008 for Neapolis, AB (Prairie Malt Ltd.). k Data not collected in 2007 for Beiseker, AB (ADM Malting Canada). false loose smut, and spot blotch; moderately resistant to moderately susceptible to netted net blotch, FHB and stem rust (carries the Rpg1 gene but susceptible to race Pgt-QCCJ); moderately susceptible to common root rot; susceptible to scald and speckled leaf blotch (Septoria passerinii Sacc.) (Table 5). Overall, Major has a superior disease resistance package when compared with the checks, particularly to spot blotch and both netted and spotted net blotch. from F9 single plant selections originally made at the AAFC Brandon Research Centre in 2006 from the same increase used to provide seed for evaluation of Major in the Western Cooperative Two-row Barley Registration Test. Distribution and multiplication of other classes of Pedigreed seed stocks will be handled by Viterra Inc., 2625 Victoria Avenue, Regina, Saskatchewan, Canada S4T 7T9. Maintenance of Pedigreed Seed Stocks Breeder Seed will be maintained by the AAFC Seed Increase Unit, Experimental Farm, Box 760, Indian Head, Saskatchewan, Canada S0G 2K0. Initial Breeder Seed was produced in 2008 by the Seed Increase Unit at Indian Head from a bulk of 199 F11 lines derived Financial support from the producer check-off on barley collected in Manitoba and Saskatchewan by the Canadian Wheat Board and administered by the Western Grains Research Foundation is gratefully acknowledged. Appreciation is expressed to D. Gehl (AAFC, Indian Head, SK) for assistance in producing and maintaining the Breeder Seed and facilitating winter nurseries; P. Green, B. McLeod and Ustilago smuts Net blotch z Can. J. Plant Sci. Downloaded from pubs.aic.ca by Agriculture and Agri-food Canada on 12/06/13 For personal use only. (% infected) nuda hordei nigra Sasky Covered 2006 Harrington Xena AC Metcalfe CDC Kendall Major 29 81 0 81 0 10.0 3.0 3.0 3.5 3.5 22.5 40.0 7.5 26.5 9.0 2007 CDC Copeland Xena AC Metcalfe Major 94 89 0 0 0.5 0.0 0.0 0.0 0.0 0.0 0.0 5.0 Cultivar Spot blotch x Inoculated Melfw (1
9) Branv (1
9) Melfw (1
9) Sasku (1
9) Inoc.t 1903 9 3 5 3 1 7.5 2.0 4.5 1.5 1.5 7.5 7.0 5.5 6.5 3.5 4.5 5.0 3.5 4.0 3.0 6.5 5.0 4.5 4.8 2.5 7 6 6 6 2 5 5 5 2 2.0 1.0 3.5 1.5 4.5 5.0 5.0 3.5 5.0 4.5 3.5 2.5 5.5 4.5 3.5 3.0 6 7 6 5 102 858 857 S S R MR R 10 10 9 5 2 10 10 10 9 8 MR S R MR 6 9 9 4 9 10 9 8 Scald r Fusarium head blight Fieldp Common Root Rot (% infected)s Stem Rust MCCF SeedlingIT Inocul.q 1493 Edmon Laco Septoriao 1998 FHBn (0
5) DONm (mg kg 1) 2006 Harrington Xena AC Metcalfe CDC Kendall Major 89 89 89 87 88 3
2 0;1 0;1 3
2 0;1 S S S S S 1.5 2.5 0.5 2.0 4.0 6.5 7.0 6.0 4.0 7.0 S S S S S 2.3 1.3 2.3 2.5 2.0 5.9 3.7 5.6 5.4 3.5 2007 CDC Copeland Xena AC Metcalfe Major 96 57 80 84 0; 0; 0; 0; S S S S 3.0 2.0 2.0 0.0 9.0 8.5 8.5 9.0



1.8 2.2 2.2 2.3 1.9 1.7 5.9 4.9 Cultivar z Infected plants (%) as determined in smut tests conducted at the Agriculture and Agri-Food Canada (AAFC) Cereal Research Centre (CRC), Winnipeg, MB. Covered smut (U. hordei) rating determined at the Crop Development Centre (CDC), University of Saskatchewan, Saskatoon, SK; Ssusceptible, MRmoderately resistant, Rresistant. x Seedlings inoculated with Pyrenophora teres f. teres isolates WRS102 and WRS858, and P. teres f. maculata isolate WRS857 from the CRC, Winnipeg, MB; 1resistant, 10susceptible. w Rated for netted net blotch (P. teres f. teres) and spot blotch (Cochliobolus sativus) reactions in the leaf disease nursery at AAFC, Melfort, SK, on a 1
9 scale (1resistant, 9susceptible). v Rated for spot blotch (C. sativus) reactions in the irrigated leaf disease nursery at AAFC, Brandon, MB, on a 1
9 scale (1resistant, 9susceptible). u Rated for reaction to spot blotch (C. sativus) in the CDC irrigated nursery, Saskatoon, SK, on a 1
9 scale (1resistant, 9susceptible). t Seedlings inoculated with C. sativus isolate WRS1903 from the CRC, Winnipeg, MB; 1resistant, 9susceptible. s Percentage of plants infected in the common root rot (C. sativus) nursery at AAFC, Lacombe, AB. r Reaction to stem rust (Puccinia graminis) race MCCF to detect the Rpg1 stem rust resistance gene in seedling tests at the CRC, Winnipeg, MB. q Seedlings inoculated with Rhynchosporium secalis isolate WRS1493 from the CRC, Winnipeg, MB; Ssusceptible. p Field ratings for scald (R. secalis) reactions on a 0
9 scale where 0no disease, 9susceptible; EdmonUniversity of Alberta scald nursery, Edmonton, AB; Laco AAFC, Lacombe, AB, scald nursery. o Seedlings inoculated with Septoria passerinii isolate WRS1998 from the CRC, Winnipeg, MB; S susceptible. n Mean fusarium head blight (Fusarium graminearum) reaction rated visually on a 0
5 scale (0no symptoms, 5susceptible) in the irrigated FHB nursery at AAFC, Brandon, MB; mean for each year calculated from 3 replications. m Deoxynivalenol (DON) concentration determined by the Enzyme-Linked ImmunoSorbent Assay (ELISA) technique at the Eastern Cereal and Oilseed Research Centre (ECORC), Ottawa, ON, using a composite sample of 3 replications for each test. y 296 CANADIAN JOURNAL OF PLANT SCIENCE Table 5. Disease reactions for Major and check cultivars, Western Cooperative Two-row Barley Registration Test, 2006 and 2007 LEGGE ET AL. * MAJOR BARLEY Can. J. Plant Sci. Downloaded from pubs.aic.ca by Agriculture and Agri-food Canada on 12/06/13 For personal use only. K. Moore (AAFC, Brandon, MB) for their technical assistance; K. Price (AAFC, Cereal Research Centre, Winnipeg, MB) for providing malting quality data; S. Buffam (AAFC, Eastern Cereal and Oilseed Research Centre, Ottawa, ON) for the DON analyses; and Dr. B. G. Rossnagel and D. Voth (Crop Development Centre, University of Saskatchewan, Saskatoon, SK) for providing field leaf disease data. Legge, W. G., Haber, S., Harder, D. E., Menzies, J. G., Noll, J. S., Tekauz, A., Thomas, P. L., Turkington, T. K. and Bizimungu, B. 2008. Newdale barley. Can. J. Plant Sci. 88: 717
723. Legge, W. G., Metcalfe, D. R., Haber, S., Harder, D. E., Noll, J. S., Tekauz, A. and Thomas, P. L. 2003. AC Metcalfe barley. Can. J. Plant Sci. 83: 381
384. 297 Legge, W. G., Therrien, M. C., Tucker, J. R., Banik, M., Tekauz, A., Somers, D., Savard, M. E., Rossnagel, B. G., Lefol, E., Voth, D., Zatorski, T., Harvey, B. L. and Scoles, G. 2004. Progress in breeding for resistance to fusarium head blight in barley. Can. J. Plant Pathol. 26: 436
442. Mather, D. E., Tinker, N. A., LaBerge, D. E., Edney, M., Jones, B. L., Rossnagel, B. G., Legge, W. G., Briggs, K. G., Irvine, R. B., Falk, D. E. and Kasha, K. J. 1997. Regions of the genome that affect grain and malt quality in a North American two-row barley cross. Crop Sci. 37: 544
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