Gastroprotective effects of Calendula officinalis Extract.pdf

Peptic ulcer is a most common ulcer of the gastrointestinal tract that is usually acidic and thus extremely painful. This pathological condition is caused by chronic inflammation due to Helicobacter pylori, excessive use of of NSAIDs like aspirin and smoking. This disorder also results in release of massive amount of toxic free radicals which results in oxidative stress. Ethnobotanically, the whole plant of Calendula officinalis has been reported to be used in the treatment of various disorders including stomach and other diseases. Antiulcer-activity of the 50% ethanolic extracts in order to validate ethnobotanical claims regarding the plant, used in the above disorders. Four groups of six albino rats in each group were used. They were pretreated with (0.25% w/v) carboxymethyl cellulose (negative control, 10 ml/kg), 50 mg/kg ranitidine (positive control), whole plant extract of Calendula officinalis (250 and 450 mg/kg/body weight) and their effect was studied on aspirin induced ulcer, cold-resistant stress-induced ulcers, pylorus ligation and ethanol-induced ulcers. The results of the present study showed that the whole plant extract of Calendula officinalis possessed gastroprotective activity as evidenced by its significant inhibition in the formation of ulcers induced by physical and chemical agents with a maximum of 87.15 % therapeutic efficiency (450 mg/kg b.w.) in cold resistant stress-induced ulcers. The present study was also aimed to investigate the effect of this extract on oxidative stress by measuring the level of various oxidative markers. The result of enzyme assay and lipid peroxidation clearly indicates the whole plant of Calendula officinalis extract have significant antioxidant effect on ulcer pathology. Whole plant extract have decreased LPO (p< 0.001) and SOD (p<0.01) with concomitant increase in catalyse activity in cold resistant stress-induced ulcers.

Centaurea chamaerhaponticum Ball. (Asteraceae), Lawsonia inermis L. (Lythraceae), Origanum compactum Benth. (Lamiaceae), Punica granatum L. (Punicaceae) and Ceratonia siliqua L. (Sapindaceae) are medicinal plants recommended in Moroccan folk medicine for the treatment of gastric ulcers. The protective effects of the studied plants against gastric damage were investigated using three experimental models in rats (HCl/ethanol, pylorus ligation, and aspirin-induced ulcer). Each plant extract (250 or 500 mg/kg) was orally administered once prior to ulcer induction. The antiulcer activity was assessed by comparing the ulcer index and gastric parameters in the test group with those of the control group. As a result, all five plant extracts showed, at the high dose, a significant gastro-protective effect. O. compactum showed the highest ulcer protective in NSAID-induced ulcer model (86.1 % of protection), greater than that of omeprazole (79.7 %) and almost similar to that of cimetidine (84.8%). The volume of gastric secretion was decreased significantly by C. chamaerhaponticum (50% of gastric secretion reduction), C. siliqua (46.4%) and L. inermis (42.8%) similarly to omeprazole’s antisecretory effect (50% of secretion reduction). A significant decrease in the total acidity was noticed only at the highest dose for all tested plants (percent of total acidity reduction ranged from 22.2 to 30.5 %), except for C. chamaerhaponticum which showed no significant modifications in both total acidity and gastric pH. Taken together, the results of this study indicate that the selected plants possess potent gastroprotective and antisecretory properties, which justify the ethno-medicinal claims.

Iranian Red Crescent medical journal, 2011

In patients with ulcerative colitis (UC), the repeated cycle of injury and repair of intestinal mucosa has been reported to increase the risk of colon cancer. So, a safe and efficient therapy is required for the treatment and prophylaxis for the disease.This study aims to investigate the efficacy of Calendula officinalis extract in treatment of experimentally induced ulcerative colitis in dog animal model. During fall 2010, 10 out-bred female German dogs (1-2 years old; weighs of 20-25 kg) were enrolled. Ulcerative colitis was induced with 6% acetic acid as enema and method of treatment was retrograde (via enema) too by C. officinalis. Loose stools, diarrhea, gross bleeding and loss of body weight happened after administration of acetic acid and crypt damage, loss of epithelium, infiltration of inflammatory cells and depletion of goblet cells were noticed histologically. C. officinalis could successfully resolve the damages of UC. Treatment with C. officinalis can broaden the curren...

Journal of Pharmaceutical and Scientific Innovation, 2013

Proceedings of the Indian National Science Academy

Journal of Pharmacy and Nutrition Sciences

Medicinal plants have been widely studied to identify plant-based anti-gastric ulcer medicines. The mechanism of gastroprotective action is important to discover the potential lead compounds for drug development. All relevant articles between 2011 and 2021 focusing on Malaysian plants were collected and analyzed to understand the underlying pathways. Keywords include peptic ulcer, gastric ulcer, NSAIDs (Non-Steroidal Anti-Inflammatory Drugs), Helicobacterpylori, medicinal plant, gastroprotection, antiulcer, acid secretion, cytoprotective, and digestion processes were applied in the search engines. Twenty-two of the plants had been reported based on the collected data. The review concludes that Malaysian plants could protect the gastric wall against necrotizing agents like ethanol and NSAIDs. This is mainly due to four critical defensive mechanisms: cytoprotective barriers, regulation of heat-shock protein 70 (HSP70) and pro-apoptotic protein (BAX), gastric acid secretion, and antiox...

Journal of Young Pharmacists, 2009

Peptic ulcer is one of the most common gastrointestinal diseases. The exact causes of peptic ulcer disease are not known but it may be result from an imbalance between acid-pepsin secretion and mucosal defence factors. Peptic ulcer disease occurs mainly due to consumption of NSAIDs, infection by H. pylori, stress, or due to a pathological condition such as Zollinger –Ellison Syndrome. Nature provides a number of medicines and perhaps all solutions for human illnesses. So far, nature supplies many clinically useful drugs. Ulcer is a major increasing health problem. Nowadays, there are several synthetic medications available for the treatment of peptic ulcers, but these drugs are expensive and are likely to produce more side effects than herbal drugs. Certain antiulcer drugs such as proton pump inhibitors and H2 receptor antagonists are used to treat peptic ulcers. However, these drugs have shown disease relapse, side effects, and even drug interactions. Numerous medicinal plants have anti-ulcer activity and are useful in the treatment of peptic ulcers. The aim of this review is to find out more about the anti-ulcer properties of herbal medicines. The present article reviews the antiulcerogenic and ulcer healing property of Ocimum sanctum, Allophylus serratus, Desmodium gagenticum, Azadirachta indica, Hemidesmus racemosus, Asparagus racemosus, and Musa sapientum. We have brought to light some of the important plants reported for their anti-ulcer and ulcer healing properties. Ayurvedic knowledge supported by modern science is necessary to isolate, characterize and normalize the active components of herbal sources for antiulcerative activity. KEYWORDS: Antiulcer activity, Ethanolic extract, Aspirin-induced ulcer model, NSAIDs.

Evidence-Based Complementary and Alternative Medicine, 2015

Calendula officinalisL. has been largely known for its topical anti-inflammatory properties; however, there are no experimental evidences about its antiphlogistic effect at the gastric level. To investigate whether marigold might exert an activity against gastric inflammation, a CH2Cl2extract obtained fromC. officinalisflowers was evaluatedin vitroon the NF-κB pathway. The lipophilic extract demonstrated a significant inhibitory effect on the NF-κB driven transcription. The identification of active compounds was conducted by a bio-guided fractionation of the extract that afforded 16 fractions. Fraction J exhibited a concentration-dependent inhibitory activity on the NF-κB driven transcription and significantly contributed to the antiphlogistic effect showed by CH2Cl2extract. The main components of fraction J were loliolide and the fucoside acetates ofβ-eudesmol and viridiflorol. HPLC analysis of fractions D and E led to the identification and isolation of triterpene esters that show...