Chela-Flores, J., Montenegro, M.E., Pugliese, N. Tewari, V.C. and Tuniz, C. (2009).
Evolution of plant-animal interactions. In: All flesh is grass: Plant-Animal Interactions, a
love-hate affair. Z. Dubinsky and J. Seckbach (eds.). Cellular Origin and Life in Extreme
Habitats and Astrobiology, Springer: Dordrecht, The Netherlands, in press.
EVOLUTION OF PLANT-ANIMAL INTERACTIONS
J. CHELA-FLORES 1,2, M. E. MONTENEGRO 3, N. PUGLIESE
V. TEWARI 1,5 AND C. TUNIZ 1
3,4
,
1
The Abdus Salam ICTP, Strada Costiera 11, 34151 Trieste, Italy, 2Instituto de
Estudios Avanzados, IDEA, Caracas 1015A, República Bolivariana de Venezuela;
3
Museo Nazionale dell’Antartide (sezione di Trieste), via Weiss 21, 34127 Trieste,
Italy; 4Dipartimento di Scienze Geologiche, Ambientali e Marine, University of
Trieste, via Weiss 2, 34127 Trieste, Italy; and 5Wadia Institute of Himalayan
Geology, 33, General Mahadeo Singh Road, Dehra Dun, 24800, India.
1. Introduction
The evolution of plant-animal interactions goes back to the Early Archean, where the
first signals of photosynthesis may have been detected in the Isua Peninsula in
Greenland, a phenomenon that is related to the isotopic anomalies of carbon. The first
evidence of reliable fossils of photosynthetic microorganisms has been identified by
micropaleontologists in the Late Archean and Early Proterozoic. A closely related topic
in this geologic time interval is the evolution of trophic relations and metabolic
diversification in the microbial world. In the context of the three domains of life,
Archaea, Bacteria and Eucarya, the bifurcation of multicellular organisms into plant and
animals becomes evident only during the Paleozoic. Cell evolution also leads up to the
unicellular dichotomy of autotrophs and heterotrophs. Symbiosis has a strong role to
play in the transition to plants and animals in the Phanerozoic. It is timely to focus on
details of evolution in the Cretaceous and Tertiary, where detailed pathways of evolution
have been gathered in many geographical regions, including the Karst region of northern
Italy. Various experimental techniques have contributed to elucidate the coevolution of
plants and animals. A special case of plant-animal interaction is the evolution and
dispersal of hominins, including their impact on the ecosystems. A significant
development in understanding the evolution of plant-animal interactions is based on the
possibility of identifying reliable biomarkers that can characterize its different stages,
from the earliest microbes to the extant plant and animals. Such identification of
biomarkers labeling different stages of evolution may orient the search for life in the
exploration of the Solar System.
2. Evolution of the microbial world in the Proterozoic-Early Phanerozoic
2.1. THE TREE OF LIFE AND LAST UNIVERSAL COMMON ANCESTOR
The dichotomy between plants and animals evolved from the microbial world that lasted
for the major part of Earth’s history (Archean and Proterozoic). The microbial cellular
2
plan consists of prokaryotes, lacking a central nucleus and eukaryotes that evolved later
containing a central nucleus enveloping the genetic material. Eukaryotes were earlier
considered similar to eubacteria and archaeobacteria and diversified from a universal last
common ancestor (Fig. 1).
Fig. 1. The relationship between the three domains of life. We have followed the original proposal and
terminology used by Carl Richard Woese and co-workers, where the nucleated cells are grouped in the domain
Eucarya, while the microorganisms themselves are called eukaryotes (Woese et al., 1977).
In the Archean biosphere, the microbial communities were dominantly marine,
however, unicellular eukaryotes must have been present. The unicellular organisms that
can survive in extreme conditions are very close to the eukaryotes evolved in
Paleoproterozoic around 2.1 billion years (Ga) before the present (BP). Cyanobacteria
were the main prokaryotic microbial fossils reported from rocks dating from 3.5 Ga BP.
Life on Earth has been classified in three domains Archaea, Bacteria and Eucarya
(eukaryotes), (Woese et al., 1990). The evolution of eukaryotes is a debatable subject.
The Archean oceans may have colonized by prokaryotes and proto-eukaryotes. The
prokaryotes are single-celled microorganisms and can be easily distinguished by their
DNA structure that is simple and not bounded by membrane and nucleus. The
mitochondria, chromosomes and chloroplasts are also lacking in prokaryotic cells.
3
The symbiosis between prokaryotes and eukaryotes resulting in modern
mitochondria and chloroplasts are well established. This remark lies at the basis of the
eventual separation of multicellular organisms between plants and animals (cf., Sec.
3.1). It is believed that the modern eukaryotic cell resulted from symbiosis of eubacterial
organelles into an Archaea-like rootstock (Margulis and Cohen, 1994). The Archaea
domain includes, amongst others, hot spring bacteria and methanogens. The Bacteria
domain includes cyanobacteria, anoxygenic photosynthetic bacteria and the
mitochondria and chloroplasts of the eukaryotes (Wheelis et al., 1992). Archaea and
Eucarya shared a common ancestor that was not shared by Bacteria. Hyperthermophiles
diverged from the Archaea-Eucarya domains (Woese et al., 1990, Wheelis et al., 1992).
Paleobiological evidence indicates that eukaryotes evolved more than 2 Ga BP.
Modern unicellular organisms surviving in the extremely hot conditions may be their
modern analogues (Tewari et al., 2008). This view differs from the earlier view that the
eukaryotes share a common ancestry with Archaea. This evidence was based on genes
that code for RNA of ribosomes in Eucarya, Archaea and Bacteria. The nuclear genome
contains genes that are of specific eubacterial origin, and some genes are specific to
eukaryotes (Woese, 1987). The first appearance of endosymbiont eukaryotes is not clear,
however, they must have originated in late the Archean. Multicellular eukaryotes
evolved later, around 1.5 - 1.0 Ga BP. These metazoans appeared as a major eukaryotic
radiation in the Mesoproterozoic period around 1.0 - 0.8 Ga BP (Knoll, 1984). Terminal
Neoproterozoic (0.65-0.57 Ga BP) was the time of Ediacaran explosion of diploblastic
animals (Conway Morris, 1989; Narbonne and Hofmann, 1987). Their possible modern
analogues are coelenterates like jellyfish, corals and sea anemones. The Cambrian
explosion of triploblastic animals with three germ layers is the major event of
multicellular metazoans. In the present paper we discuss physical and paleobiological
evidences of prokaryote to eukaryote evolution on Earth. The presence of
microorganisms in Antarctic lakes including eukaryotic diatoms and cyanobacteria not
only supports the idea that life can survive in extreme environments, but also that life
may thrive on Mars and Europa (Chela Flores 1998; Chela Flores et al., 2008; Tewari,
1998, 2001a).
2.2 EVIDENCE FOR THE PRECURSORS OF PLANTS AND ANIMALS
Life is generally accepted to have evolved on Earth between 3.8 and 3.77 Ga BP, but an
earlier onset of life cannot be excluded. This would have important implications for the
current debate on the possibility of temporal overlap of earliest life with a bolide impact
scenario terminating at ~3.8 Ga BP (Whitehouse et al., 1999, Moorbath, 2005). The
early organisms probably formed around submarine hydrothermal vents. Prokaryotes are
the oldest known organisms on Earth and constitute about 70% history of life till
eukaryotes appeared around 2.1 Ga BP. The fossil bacteria are found well preserved in
black cherts associated with stromatolitic carbonates in most of the Archean and
Proterozoic period (Schopf, 1993, 1994; Knoll and Walter, 1992, Tewari, 2004, Shukla
et al., 2006). Westall (1999) has shown experimentally that earlier eukaryotes were
identified under thin sections mainly by internal spots as the supposed remains of
organelles, tetrahedral tetrads of cells, scars indicative of meiotic cell division, large cell
size, and mitotic cell division; however, these features are reported from silicified
bacteria. Tewari (1989, 2004) has also reported these features from the Deoban cherts of
4
the Lesser Himalaya. Thus it is difficult to recognize the exact time of the first
appearance of eukaryotes in fossil records. Cyanobacteria from the early Archean rocks
of Australia and South Africa have been reported from the hydrothermal environment
(Schopf, 1993; Westall et al., 2001).
The microbial assemblage was photoautotrophic, but the oxygen level was rather low
in the Achaean period. The Proterozoic microbiota is highly diversified and well
preserved in subtidal to intertidal cherts associated with microbially mediated
stromatolites (Tewari, 2004, 2007, Schopf et al., 2008). Meso- to Neoproterozoic
Deoban and Buxa stromatolitic carbonates of the NW and NE Lesser Himalaya are
classical example of preservation of these microfossils (Tewari, 1989, 2004, 2008,
Shukla et al., 2006, Schopf et al., 2008). The atmospheric oxygen increased during
Paleoproterozoic to Mesoproterozoic around 1.9-1.6 Ga BP and perhaps eukaryotes
started appearing as leiosphaerid acritarchs in China (Zhang, 1984). Eukaryotic algae are
known from 2.0 Ga BP Duck-Creek Dolomite of Western Australia. The diversity in
fossil microorganisms is also environmentally controlled in some classical sections like
Draken Formation of Spitzbergen (Knoll and Swett, 1987) and Deoban-Blaini-Krol-Tal
Formations of the Lesser Himalaya (Tewari, 1989, 2001b, 2004, 2007). Subtidal to
intertidal stromatolites and microbiota of Deoban Group include: Kussiella kussiensis,
Colonnella columnaris, Baicalia nova and Tungussia (stromatolites) and eukaryotic
algae Kildinosphaera along with Eomycetopsis robusta, Siphonophycus kestron,
Oscillatoriopsis media and others (Tewari, 1989, 2004 and references therein).
Eukaryotic algae diversified in open marine tidal flat environment.
The discovery of Vendotaenia (and a new genus Krolotaenia gniloskayi) from the
Lesser Himalaya shows that eukaryotic alga evolved during Ediacaran or
Vendian/Terminal Proterozoic period and the Vendotaenid assemblage coincides with
Ediacaran assemblage in the Krol Formation of the Lesser Himalaya, India, Canada and
elsewhere (Tewari, 1988, 1989, 1993, 1999, 2004, 2007; Hofmann, 1994). The Krol
Formation also contains Neoproterozoic diverse organic walled microfossils,
stromatolites, and Ediacaran metazoans (Tewari, 2004 and references therein).
Vendotaenia is also found in Dengying Formation of China in association with
eukaryotic sphaeromorphic acritarchs. This is a coevolution of plants and animals during
Ediacaran/Vendian times (650 – 540 million years before the present, Ma BP, after the
Neoproterozoic glaciation best known as ‘Snow Ball Earth, Tewari’, 2001b). Recently,
we have discussed the δ13C and δ18O during Meso- to Neoproterozoic period in the
Lesser Himalaya with reference to global paleoclimatic events, paleobiological
evolutionary changes and their global correlation (Tewari (2007; Tewari and Sial, 2007).
Ediacaran metazoans were diploblastic animals and related to the increase in the
atmospheric oxygen of about 4 — 8 % of the present atmospheric level (PAL).
Triploblastic organization of these metazoans is further increased by atmospheric
oxygen level up to 10 % PAL. Cambrian radiation event of multicellular life is linked
with 40 % PAL around 0.57 — 0.5 Ga BP. Prokaryotic to eukaryotic cellular
evolutionary change has taken place during one billion years of Earth’s early history.
Additional micropaleontological discoveries combined with the use of recent
instrumental techniques like Laser Raman Spectroscopy and Confocal Laser Scanning
Microscopy (three dimensional imaging) of microfossils (Schopf et al., 2002; 2008, in
press) is important to establish the biogenicity of early life and the presence of organic
matter (biomolecules) in them. Further detailed Carbon isotope chemostratigraphy of the
key stratigraphic boundaries like Proterozoic—Cambrian (Tewari and Sial, 2007,
5
Tewari, 2007) and Cretaceous—Tertiary boundary in the Karst region of northern Italy
(Tewari et al., 2007) will eventually shed more light on evolution, diversification and
catastrophic extinction of life on Earth.
2.3 THE EVOLUTION OF TROPHIC RELATIONS SINCE THE ARCHEAN
A closer and more detailed understanding of the origin of life on Earth has forced upon
us a more significant view on the Late Archean and Early Proterozoic evolution of
trophic relations in the microworld.
Presently we are more aware that hydrothermal vents in the Earth oceans may have
played a role in the origin and evolution of the three domains of life (cf., Fig. 1). Indeed,
it is possible that throughout evolution entire ecosystems depend on geothermal, rather
than solar energy. This is not only evident on the Earth, but this is also likely to be the
case on the other oceans of the solar system, as for example on the moons of Jupiter:
Europa and Ganymede. On these satellites this particular hypothesis for the origin of life
may be tested in the foreseeable future. The Europa-Jupiter System Mission is now
being seriously considered by the main space agencies of Europe, the United States,
Japan and Russia, after the initial proposal of the LAPLACE mission (Blanc et al.,
2009).
The primary sources of organic matter for the microbial autotrophs are
photosynthesis, methanogenesis and sulfate reduction. In the limited space available it is
most important to single out and highlight that the group of sulfate reducers may contain
some of the earliest forms of life on Earth. Thus, this special form of metabolism may
hold the key to understanding the primordial state of life, since sulfate-reducers are in
deeply rooted branches of the phylogenetic tree of life (Shen and Buick, 2004).
The morphological simplicity of the primitive sulfate reducers is one drawback in
probing the fossilized remains of these microbes. Instead we must rely on the science of
biogeochemistry when our objective is to enquire on the antiquity of life and its trophic
relations. From the early papers of Manfred Schildowski and co-workers the stable
isotope geochemistry of sulfur and the other biogenic elements (H, C, O, N) has been
reviewed extensively (Schildowski, 1983; Strauss and Beukes, 1996).
For a proper understanding of the Archean S-isotopic record we should first realize
that the abiotic fractionation of carbon isotopes can produce effects comparable to
geomicrobiological effects, as described in detail elsewhere (Horita, 2005). However,
the situation is more favorable for sulfur. Indeed, microorganisms mediate the reduction
of sulfate to sulfide. The resulting fractionations can be reliably taken as good markers
for the geological record, especially for the Archean S-isotopic record, where we have
hinted that the oldest signatures for life are to be retrieved. The biology that underlies
this significant aspect of our quest for the evolution of trophic relations is as follows: the
preferential use of 32S over 34S by microorganisms deplete the sulfide in the environment
of 34S with respect to the original sulfate. Several species of bacteria and Archea can
make this happen via the metabolic pathway known as dissimilatory sulfate reduction.
Sulfur itself is not incorporated into cell, but it ends up in the oxidation of organic
matter. In normal marine sediments of sulfate, the fractionation can range from 10 to 49
‰, but this effect can be as large as 70 ‰. On the other hand, unlike the case of carbon
described above, abiotic isotope fractionations can yield fractionations in the range 1520 ‰, for example in the magmatic reduction of gaseous sulfate to hydrogen sulfide
6
(Rollinson, 2007). This leaves an ample margin for distinguishing the microbial activity
in rocks at a hydrothermal vent and the abiotic fractionations.
The S-isotopic record of sulfide and sulfate in Archean sedimentary rocks ranges
from Isua of ~3.8 Ga BP (pyrite in banded-iron formations) and ~3.47 Ga BP (barite
deposits). In these early times the sulfate reducers were beginning to leave measurable
traces, but some difficulties have still to be fully understood, as to their sources and the
role of the atmospheric contributions. In the more recent pyrites in black shales of ~2.7
Ga BP (Shen and Buick, 2004, Fig. 6), where the traces are better understood. In
conclusion the stable isotope geochemistry of the ~3.47 Ga barite deposit suggests that
reactions mediated by microorganisms were already fractionating sulfur much in the
same way as present day sulfate-reducing microorganisms.
2.4 METABOLIC DIVERSIFICATION
In the previous section we have sketched some of the most ancient microbes lying
deepest at the root of the tree of life. We proceed to discuss briefly how the evolution of
the Earth atmosphere repressed these Archaea into distinct niches. The main driving
force was the gradual oxygenation of the atmosphere after the discovery of
photosynthesis. Ecosystems adopted a layered mat-morphology and sediments. Such
ecosystems are well known in present day environments, such as the dry valley lakes of
Antarctica.
The microbial mats found today in these environments are composed primarily of
cyanobacteria, heterotrophic bacteria, protozoan cysts, eukaryotic algal cells and
minerals associated with microbial activity occurring throughout much of the benthic
regions of the dry valley lakes (Wharton et al., 1983; Mikell et al., 1984; Vincent, 1988).
In the Archean the photosynthesizers were distributed in the upper layers, while the
anaerobic microorganisms, such as the sulfate-reducing Archaea were relegated to the
lower layers. The eventual consequence of this atmospheric factor was not only to
segregate the sulfate reducers (and others such as the methanogens) to lower layers of
the mat formations, but to these microorganisms were further relegated to restricted
niches. Consequently it was inevitable that evolutionary diversification would follow.
Indeed, today we have a large number of obligate anaerobes, not only Archaea, but also
mesophilc bacteria. The microfossil record testifies that as oxygenation was gradually
driven towards PAL by ~2.1 Ga BP, sulfate reducers and methanogens left their imprint
supporting the general outline of Darwinian evolution of the three main domains of the
tree of life (Fig. 1). Beyond the consequences of natural selection and adaptation, a new
force in evolution, symbiosis, emerged that was to play a crucial role in the eventual
dichotomy of multicelullar life: plants and animals as we shall discuss in the following
sections.
3. Cellular evolution and the dichotomy plant-animals.
3.1 THE ROLE OF SYMBIOSIS IN EVOLUTION
We may separate living organisms in two large groups: prokaryotes (Bacteria and
Achaea) and eukaryotes (Encarta, cf., Fig 1 and Sec. 2.2), the first group consists
7
exclusively of unicellular organisms, considered vegetal organisms since immemorial
times (Gerona, 1988) and the second, on the contrary, consists of all multicultural plants
and animals inhabiting the Earth. On the base of this division we assume that bacteria
and unicellular algae, in their quality of autotrophic organisms are considered vegetal
organisms, and we focus our attention on the importance of the interactions of these
organisms among themselves, with eukaryotes, and with the multicultural organisms.
Frequently these interactions have developed along the history of life a sort of
dependence, which very often comes into a real co-evolution for both organisms. The
interactions between unicellular autotrophic organisms among them, and between
prokaryotes and eukaryotes (unicellular and multicultural), play a major role in
speciation and evolution.
In general, the close ecological relationship between the individuals of two (or more)
different species is defined as symbiosis. Ecologists use a different term for each type of
symbiotic relationship: mutualism is defined as the symbiotic relationship between the
individuals of two (or more) different species, where both species benefit;
commensalisms implies only one of the interacting species benefits, the other is
unaffected; finally, parasitism consists in one species benefits as the other remaining
harmed. Microbial symbiosis is known today to be a ubiquitous aspect of life.
According to contemporary conceptual consensus, the mitochondria of all eukaryotic
cells and the chloroplasts of plants and protests were once free-living bacteria (alphaproteobacteria and cyanobacteria, respectively) that became incorporated in a primitive
host cell (Gray, 1992; Margulis, 1992). It was first suggested that chloroplasts originated
as symbionts (Schimper, 1883). The concept was further developed by Merezhkowsky
(Sapp, 2005), who coined the word “symbiogenesis” for such a synthesis of new
organisms. He maintained that nucleus and cytoplasm had originated by symbiogenesis.
Microbial evolutionists also consider whether the cell nucleus may have also arisen by
some sort of fusion of symbiosis between two different kinds of bacteria.
Neodarwinism considers the Cambrian explosion as the “big bang” of biology.
During this period, between 560 and 495 Ma BP, many now-extinct plants and animals
burst onto the scene. Why the Cambrian explosion occurred is not fully understood, but
at the cellular scale the real “big bang” for plants and animals occurred some 1800 Ma
earlier, with the first appearance of eukaryotic cells (Sapp, 2005). With its membranebound nucleus and all the associated features, such as mitosis, meiosis, and multiple
chromosomes to package tens of thousands of genes per cell, it provided the material
and the conditions for the differentiation of tissues, organs, and organ systems of plants
and animals. Symbiosis is at the foundation of our being. Multicellular organisms,
humans included, probably evolved and were maintained by bacteria.
Symbiosis has not only played a principal role in the emergence of eukaryotes, it has
been vital throughout eukaryote evolution. Although eukaryotes are the most
morphologically complex microbes with the largest biomass on earth, have the greatest
biochemical complexity (Whitman et al., 1998). Microbial symbionts perform many
chemical reactions that are not possible for their hosts. Collectively they can
photosynthesize, fix nitrogen, metabolize sulfur, digest cellulose, synthesize amino
acids, provide vitamins and growth factors, and ward off pathogens.
The fact that microbial symbiosis is a fundamental aspect of life was first suggested
by botanists of the late 19th century. The dual nature of lichens, nitrogen-fixing bacteria
in the root nodules of legumes, fungi in the roots of forest trees and orchids,
photosynthetic algae living inside the bodies of protists, hydra, and the flat worm
8
Convoluta roscoffensis, suggested a temporal continuum of dependency of microbe and
host from transient to permanent interdependence. When these phenomena were
considered together with cytological evidence for reproducing organelles within the cells
of plants and animals, they led several biologists of the late 19th century to a conception
of the cell itself as a symbiotic community (Sapp, 1994). Speciation induced by parasitic
or mutualistic symbionts has been suggested for taxa ranging from plants to insects to
monkeys (Thompson, 1987). Models for symbiont-induced speciation have been
proposed based upon hybrid inferiority and selection for reinforcement genes. However,
taken on their own, such models have severe theoretical limitations and little empirical
support. Thompson highlighted the importance of the environment on the symbiontinduced speciation. He established two conditions for symbiont-induced speciation:
firstly, interaction norms in which the outcomes of host/symbiont interactions differ
between environments; and secondly, differential coadaptation of host and symbiont
populations between environments or along an environmental gradient. Thompson
defined symbiont-induced speciation as a form of ‘mixed-process coevolution’, namely,
reciprocal evolution in which adaptation of a population of one species to a population
of a second species (or co-adaptation of the populations) causes the population of the
second species to become reproductively isolated from other populations.
The term ‘symbiosis’ was introduced from the Greek language (“living together”)
into biology by Anton de Bary in 1879 (Darlington, 1951). He was discussing the
presence of algae in the leaves of the aquatic fern Azolla and the stunning discovery that
lichens were double organisms, combinations of algae and fungi. Today we know that
all of the 15,000 species of lichens are made of fungi and either algae or cyanobacteria.
A whole new structure, the thallus, emerges out of their association. For de Bary, lichens
and other examples of symbiosis offered proof of evolution, and they indicated a means
of macroevolutionary change in addition to Darwinian gradualism, based today on gene
mutations and recombination.
Frank (1885), who had used the term symbiotismus a year before de Bary, reported
another important symbiosis between fungi and the roots of forest trees, an association
that he named “mykorrhizen” (fungus root). Although the discovery of the mycorrhiza
and its ubiquity is generally attributed to Frank, the claim that root fungi may be
beneficial to plants had been made a few years earlier for the Indian pipe, Monotropa
hypopitys (Kamienski, 1881; Trappe and Berch, 1985).
Mycorrhizas are known to occur in practically all terrestrial plants: the plants benefit
from receiving essential nutrients, and the fungi benefit by receiving organic compounds
from the plant (Allen 1991; van der Heijden and Sanders, 2002). This symbiosis is
thought to have been instrumental in the colonization of land by ancient plants some 450
Ma BP (Malloch et al., 1980; Redecker et al., 2000).
Mycorrhizas are also at the very foundation of the world’s most complex and
biodiverse ecological systems. Trees of tropical rain forests, in nutrient-poor soils, rely
on these fungi to bring them precious nitrogen and minerals (Went and Stark 1968).
Nitrogen-fixing bacteria in the root nodules of legumes have also been central to
discussions of symbiosis since the 19th century. Because plants cannot metabolize the
molecular nitrogen so abundant in the atmosphere, they require nitrogen in the form of
nitrate converted to NH4+. Legumes overcome this problem by their symbiotic union
with a group of N-fixing bacteria, rhizobia, which live in specialized root nodules, where
excess NH4+ is exchanged for sugars exuded by the plant. Symbiotic N fixation
underpins the whole global N cycle. During the production of the nodule there is gene
9
transfer between host and bacteria: some of the plant DNA is transmitted to the bacteria
(Pirozynski, 1991). It is not known whether the reverse is also true (Quispel, 1998).
For plants, the associations with fungi and bacteria were the innovative key in the
colonization of land and of specific habitats. The eukaryote-associated microbes act as
metabolic partners for accessing limiting nutrients and also as protectors, producing
toxins that ward off herbivores or pathogens. Similar associations have arisen with
animals, allowing colonization of diverse niches, such as the specialized feeding on
plant or animal tissues, and the use of deep-ocean hydrothermal vent habitats. Often, the
associations are persistent for the hosts, frequently being transmitted vertically across
generations, from mother to progeny. The symbionts may be sufficiently fused that they
cannot live apart, or be easily recognized as distinct entities (Moran, 2006).
3.2 EFFECTS OF SYMBIOSIS BETWEEN AUTOTROPHS AND HETEROTROPHS
The physiological and morphological effects of symbiosis between photosynthetic
autotrophic organisms (plants) and heterotrophic organisms (animal) are varied. Sea
anemones, hydra, giant clams, sponges, and tropical corals capture algae from the ocean,
harbor them in their cells, and are nourished by their symbionts’ photosynthetically
produced carbon compounds. Corals acquire up to 60% of their nutrition from
Symbiodinium, which in return obtain from the coral polyp nitrogenous compounds that
are scarce in the crystal-clear tropical waters. A worldwide catastrophe for tropical
corals has resulted from disrupting this delicate balance (Hughes et al., 2003). Prolonged
high sea-surface temperatures, sedimentation, and inorganic pollutants cause coral
“bleaching”: corals lose their algae, leaving their tissues so transparent that only the
white calcium carbonate skeleton is visible. Without the algae, corals starve to death.
Lichens are intimate and long-term symbioses of photosynthetic algae or
cyanobacteria and heterotrophic fungi. As intimate symbioses where the photosynthetic
partner is inhabiting its heterotrophic partner (Law and Lewis, 1983; Ahmadjian, 1993),
lichen symbionts are often hypothesized to have undergone long-term coevolution,
especially when one or both symbionts appear obligate and specialized (Ahmadjian,
1987). However, coevolution has not been rigorously tested for lichen associations
(Michele et al., 2001).
To demonstrate coevolution directly requires an assessment of increased fitness
resulting from reciprocal genetic change (Thompson, 1994), although coevolution could
be demonstrated indirectly by showing parallel cladogenesis or cospeciation between
symbiont lineages (Page and Hafner, 1996). A hypothesis of parallel cladogenesis would
be accepted with highly specific associations between algal and fungal partners,
especially if there is strict vertical transfer of inhabiting algal partners throughout a
fungal lineage. In contrast, this hypothesis would be rejected in the case of horizontal
transfer of algal partners among fungal lineages. This phylogenetic process is here called
algal switching.
When reciprocal evolution leads to cospeciation, coordinated speciation events,
equal numbers of species among symbiont partners should evolve—a situation not
predicted from morphological studies of these algae (Tschermak-Woess, 1988) and their
fungal partners (Hawksworth et al., 1995).
10
3.3 THE ROLE OF THE ENVIRONMENT IN COEVOLUTION
Evidence is accumulating that interactions of animals with environmental microbes have
resulted in the coordinate evolution of complex symbioses, both benign and pathogenic
(McFall-Ngai, 1998; Henderson et al., 1999; Hooper et al., 2001), and that coevolved
animal-bacterial partnerships represent a common, fundamental theme in the biology of
animals. Most environmentally transmitted symbioses in animals, such as the alimentary
canal and squid–vibrio associations, are extracellular and often remain open to the
environment throughout the life history of the host. Thus, the host/symbiont community
must not only develop mechanisms by which to achieve a balanced, functioning
population ratio but it must also ensure specificity of the interaction from the inception
of the relationship throughout its persistence. Available evidence suggests that such
controls are mediated by: the direct interaction of the bacterial cells with the host cells
that are colonized; and by the immune system, both innate as well as adaptive (when
present), which samples the population and keeps the host informed of the state of the
interaction (McFall-Ngai, 2001). For example, interactions with the gut microbiota
induce the mammalian intestinal mucosa to produce mucins and alpha- defensins, which
inhibit the symbionts from invading host tissues (Hooper et al., 2001).
We remark, then, that humans are also engaged in several mutualistic relations with
other species (cf., Sec.5). These relations range from our gut flora (without which we
would not be able to digest food efficiently) and our immune system, to domesticated
animals like dogs or horses. These interactions provide protection, or other services in
return for food and shelter. Furthermore, man takes benefits from mutualism of other
species (second degree mutualism): in traditional agriculture men exploits the
mutualistic beneficial functions of many plants as companion plants, providing each
other with shelter, soil fertility and the repelling of pests, for example, the cultivation of
beans, which may grow up cornstalks as a trellis while fixing nitrogen in the soil for the
corn. Therefore, symbiosis is recognized to be a ubiquitous aspect of life. The
mechanisms at the origin of these relationships and how and why species might
cooperate is a question that has been raised by a number of writers who philosophically
question the conception of evolutionism and the notion of linear historical progress.
4. Evolution in the Cretaceous and Tertiary of the Trieste Karst
4.1 EXAMPLES OF PLANT-ANIMAL INTERACTIONS
The geological history of the Karst area encompasses a time spanning from the early
Cretaceous to the mid-Eocene (Cucchi et al., 1987; Pugliese and Tunis, 2006). This
stratigraphic sequence includes carbonate deposits (from the early-Cretaceous to the
mid-Eocene partim) overlaid by mid-Eocenic torbiditic beds (marls and sandstones of
the flysch). Most carbonate beds record shallow water marine-to-lacustrine settings
where the development of the relationships between plants and animals took place.
Considering the carbonate sequence, paleontologists may propose examples of these
relationships and reconstruct some hypothetical environmental scenarios through the
analysis of well-defined fossil taxa. The most representative taxon was the calcareous
microalgae (especially Cyanophyta, Dasycladaceae, Charophyta, Corallinaceae), organic
builders (including rudists and corals) and microfaunae (benthic foraminifers).
11
Calcareous algae populated several shallow substrates of the depositional
environments recorded in the Karst stratigraphic sequence. The main taxa correspond to
Cyanophyta, Charophyta, Dasycladaceae and Corallinaceae. The calcareous algae may
represent a favorable basis for a food chain and a good house for the animals.
Rudists and subordinately corals were the organic builders (especially requienids,
radiolitids and hippuritids). Organic builders are usually symbiont-bearing taxa.
Symbiosis consists of a host-symbiont interdependence between them and the
microalgae. This interdependence is advantageous for both: a symbiont gets nutrients
and place to live; the host gets food and oxygen. Moreover, photosynthesis induces
calcification in settings that are poor in carbon dioxide. However, there is no data about
the symbiosis between the Cretaceous rudists and microalgae. Corals were sporadically
present in both Cretaceous and Tertiary beds and produced small build-ups, especially
during the Tertiary. Similar to the modern corals, they can be considered symbiontbearing taxa (cf., for example, Pearse and Muscatine, 1971).
Foraminifers are usually representatives of the most abundant taxon in the fossil
record. They show several kinds of relationships. Like corals, the larger foraminifera are
a symbiont-bearing taxon (cf., for example Hohenegger, 1999). Moreover, following
Langer (1986, 1993), who studied the recent foraminifers from some Mediterranean
sectors, we focus on the adaptation of the epiphytic foraminifers to a well-defined
microenvironment:
A. Permanently immobile (flat, trochospiral, conical) taxa, that live attached to plants;
some arborescent taxa live on shaded parts (rhizomes) of plants.
B. Temporarily mobile conical, trochospiral taxa that glide over flat substrates (algae,
sea-grasses) extending their pseudopodia toward moving direction.
C. Mobile taxa living within algae that build three-dimensional meshworks; some planespiral, or annular species, suspend their tests and, like the spiders, produce a
pseudopodial network to capture their food.
D. Mobile ovate, elongate or planispiral taxa living over the plants.
4.2 HYPOTHETICAL ENVIRONMENTAL SCENARIOS
Considering the previously described examples of relationships plants-animals, some
environmental scenarios can be interpreted as follows:
4.2.1 Low-hydrodynamism lagoons (inner lagoons)
More or less restricted lagoons, mostly occurred in the Cretaceous and Paleocene beds,
evidencing anoxic, or oxygen-deficient conditions. In particular:
• Early Cretaceous dark limestones, are usually present an oligotypical biota, consisting
of opportunistic taxa and limestones with calcareous algae (Cyanophyceae,
Thaumatoporella parvosiculifera, rare Dasycladaceae). Also stromatolitic layers suggest
shallow vegetated bottoms, where animals grazed. Thin-shelled both vegetarian and
predator gastropods might have lived in these environments. Vegetated bottoms may be
suggested by foraminifers that were mostly represented by morphotypes D (mobile small
Miliolidae) and A (arborescent Nubeculariidae, and rare small cone-shaped species). The
organic builders activity is sporadic (and is due to the requienids) maybe in relation with
an increase of the hydrodynamism and the related temporary improvement of
oxygenation. Caffau et al. (2000) described the corresponding biota that was
12
characterized by the requienids giving rise to thin banks. Moreover, a relatively rich
biodiversity emerged: foraminiferal morphotypes A (Nubeculariidae), D (small
Miliolidae) suggests possible vegetated bottoms, in the presence of grazing gastropods.
Just above the early Cretaceous limestones, a Cenomanian dolomitic unit testifies
very restricted conditions, in relation with a sea-regression. It represents extreme lifeconditions (innermost lagoon, tidal pool), where only gastropods and the agglutinated
foraminifer Glomospira have been found. It is presumable that these very restricted
settings might have been occasionally vegetated, as also demonstrated by sporadic
stromatolitic layers that may have been colonized by grazing taxa.
The boundary between this unit and the overlying late Cenomanian dark limestones
is marked by an interval characterized by paleosoils. The dark limestones correspond to
a renewal of the benthic life that was linked to a transgressive phase. The community
showed opportunistic taxa very similar to those already observed in the early Cretaceous
unit.
Algae (Cyanophyceae and T. parvosiculifera) might have provided a favorable
substrate for prey/predators life, also in the presence of the most opportunistic
radiolitids, which gave rise to small patch-reefs. Within a better-diversified biota, the
foraminifers showed possible morphotypes A (Nubeculariidae), B (Nezzazatidae), C
(Biplanata peneropliformis, Biconcava bentori, Broeckina balcanica) and D
(Spiroloculina, small Miliolidae), suggesting a vegetated nature of the substrate.
During the Senonian, some levels were characterized by poor-oxygen conditions
showing opportunistic biota. They consisted of taxa that were similar to those found in
the early Cretaceous. Brackish water wetlands occurred during the Danian and
Selandian. Most beds yield small foraminifers represented by the morphotypes B
(discorbids), C (Bangiana hanseni) and D (small Miliolidae). In relation with the fresh
water influence, the scenario also included Charophyta, mostly thin-shelled gastropods
and ostracods, together with very rare foraminifera (morphotype B, discorbids).
Charophyta were represented by their girogonites, encrusted stems and Lagynophora
that probably produced meadows, where animals grazed.
4.2.2 High-hydrodynamism lagoons (open lagoons)
These settings are represented mainly by Senonian (partim) and Tertiary limestones with
a high fossiliferous content. They present a very rich life including algae, foraminifera
and mollusks.
The late Cretaceous beds include the alga T. parvovesiculifera and a rich rudist fauna
(radiolitids and, subordinately, hippuritids and requienids). Foraminifers were dominated
by mobile morphotypes C and D (Moncharmontia apenninica and Spiroloculina, and
Miliolidae, respectively) with rare morphotypes B (Stensioina surrentina). Among the
mollusks, the gastropods were sometimes abundant, maybe grazing on the vegetated
substrates, as also suggested by the presence of epiphytic foraminifers. In addition,
radiolitids and requienids provided a builder activity scenario.
The limestones from the Danian to Thanetian contain abundant algae that were
mostly represented by Dasycladaceae. Foraminifers were constituted by abundant
miliolids (morphotype D) and agglutinated and rotaliid species. Moreover, isolated and
colonial corals were present during the Danian. They were rare in some thin beds of the
Padriciano section (Pugliese et al., 2000) and common in Slovenia beds (Drobne et al.,
1988). Thus, it is presumable that a zooxanthellae/corals symbiosis occurred.
13
4.2.3 Peritidal settings
Their most common findings are signaled just below and above the K/T boundary. Their
sequences were repeated several times, denoting changes of intertidal/subtidal/supratidal
biota. Concerning the aqueous assemblages, the remnants obviously testify unstable
environmental conditions, where an opportunistic biota of lagoon occurred. In particular,
lagoon layers contain foraminifers represented by mobile morphotypes (B. hanseni and
small Miliolidae, in Tertiary beds) and grazing thin-shelled gastropods and ostracods.
Wherever present, the stromatolites testified a substrate favorable for grazing
gastropods.
4.2.4 Open marine settings
Ramps and in general coastal settings are common during the Senonian and after the
Thanetian. The limestones often present a high bioclastic content, where only rare
corallinacean algae were found.
Senonian biota was well diversified. In agreement with Caffau et al. (2004), the
Senonian ramps included organic builders consisting of hippuritids, sometimes together
with radiolitids and requienids. Moreover, rare zooxanthellate thamnanasteroid corals
occurred (Russo, personal communication, 2004). Among the foraminifers, the mobile
lenticular porcelaineous macroforaminifer Keramosphaerina tergestina was present.
This scenario corresponds to an oligotrophic scarcely vegetated bottom. Moreover,
corallinacean algae may have highlighted an epibiosis: they might have encrusted shells
of other taxa.
Tertiary ramps and coastal marine settings were characterized by limestones with a
more or less high muddy content. Corallinacean algae and larger foraminifera were very
common. Foraminifers were probably mobile morphotypes (large Miliolidae,
Alveolinidae, Nummulitidae and Orbitolites complanatus). Thamnasteroid
zooxanthellate corals (Turnšek and Drobne, 1998) and rare sea-urchins were present.
This scenario suggests oligotrophic conditions.
4.3 SYMBIOSIS SCENARIOS
Karst limestones yield a host-symbiont system in several horizons of its stratigraphic
sequence. The existence of these systems may be confirmed, in agreement with the
taxonomic uniformitarian (sensu Dodd and Stanton, 1990). Actually, modern corals and
larger foraminifera are symbiont-bearing taxa (cf., for example: Pearse and Muscatine,
1971; Hohenegger, 1999). Obviously, skeletal parts of the symbiont-bearing taxa can be
easily fossilized.
This manner of plant-animal interaction took place during episodes of environmental
stability. This stability corresponds to environments populated by a well-diversified
biota, consisting of several species in equilibrium. Following Travé et al. (1996),
Hottinger (1998), Hottinger and Drobne (1988) and Drobne et al. (2000), this biota was
k-strategist (sensu Dodd and Stanton, 1991). This biota is well recorded in the deposits
of ramps and, in general, in the coastal settings during the Senonian, the Thanetian and
after the Thanetian. Senonian ramps included mainly hippuritids, together with
radiolitids, thamnanasteroid corals, some Corallinaceae and foraminifers, such as the
lenticular porcelaineous larger foraminifer K. tergestina. Thus, corals and larger
foraminifera might testify a symbiosis zooxanthellae/corals and zooxanthellae (or other
14
algae)/larger foraminifera. Tertiary ramps-coastal marine settings confirm plant-animal
interactions that are described for the Senonian. Corals (Turnšek and Drobne, 1998;
Hohenegger, 1999) larger foraminifera (Alveolinidae, Nummulitidae, Orbitolites) were
in symbiosis with algae.
If the open lagoons, ramps and shallow marine settings represented stable
environments with a well-diversified biota, the more or less restricted lagoon testified
unstable settings. The former settings showed interactions based upon a K-strategist
biota, together with the possible and subordinate coexistence with r-strategists. The latter
settings evidenced r-strategist opportunistic taxa, only. In this regard, data concerning
possible r-strategists as symbiont-bearing taxa are not confirmed.
Larger foraminifera/microalgae systems may also provide detailed paleo-depth data.
Hohenneger (1999) proposed a precious synthesis of the extensive literature on larger
symbiont-bearing foraminifera. In general, foraminifera should protect the symbionts
from the intense illumination in a shallow-water environment.
Moreover, the presence of zooxanthellate hermatipic corals constitutes a geological
thermometer. In fact, the system zooxanthellae-corals is active if the sea-water
temperature is at least 18°C., in relation with the presence of an anhydrase enzyme, that
inhibits the system for temperature less than 18°C. The existence of the host-symbiont
system was surely inhibited during oxygen-poor conditions (inner lagoon, wetlands,
peritidal settings). Moreover, within the paleo-environmental evolution recorded by the
Karst rocks, the impact of the meteorite in correspondence of the K/T transition
produced remarkable effects. Among the evidence of this event, δC13 showed a negative
shift, denoting a decrease of the photosynthesis activity (Pugliese et al., 1995) and the
possible occurrence of host-symbiont systems.
5. Interaction of plants and animals since the appearance of hominins
5.1 THE FIRST HOMININS
Hominins emerge in the animal kingdom around 6-7 Ma BP. The hominin clade
includes the humans and all their ancestors back to the human-ape separation. They are
identified by two main traits from the great apes: bipedalism, (with the associated
differences in pelvis, spine, knee and foot) and skull shape (with the associated change
in brain size and shape), both probably induced by evolutionary effects that can be
classified as animal-plant interaction.
Australopithecins are the hominins preceding the homo species and showing their
presence in the fossil record between 4 and 1 Ma BP. They have characters common to
apes, such as the small 300-400 g brain, and to humans, such as bipedalism and small
teeth. During times when australopithecines were in their hey day, around 3.5 - 3 Ma
BP, the Sahara desert did not exist and these hominins could move freely in a vast land
of forests and savannahs extending from the Cape of Good Hope to Java. In fact, already
3.5 Ma BP, australopithecines were present not only in southern and eastern Africa, but
also 2500 kilometers west, in Chad. One of the oldest Australopithecus Little Foot the 4
million-year old hominin found in Sterkfontain (Clark, 1999). The anatomy of the ankle
joint shows Little Foot was bipedal but able to climb in trees by virtue of a divergent big
toe. He felt comfortable walking upright, but he might have escaped back into the trees
for safety or foraging. Australopithecines were well adapted to bipedalism, but were not
15
consistent bipeds. A nearly juvenile complete A. afarensis (nicknamed Selem), was
found in 2005 at Dikika, 4 km from where Lucy, the most famous 3.5 million years old
Australopithecus was found. Its body confirms the mosaic evolution patterns found in
other australopithecines (Alemseged et al., 2006). The passage from quadrupedalism to
bipedalism started in the lower limbs and pelvis, keeping the primitive characters in
arms and shoulders.
One of the differences between Homo and Australopithecus is the smaller size of
Homo’s jaw muscles. Australopithecines needed powerful muscles for processing nuts
and other hard foods that became necessary after the environmental change in Africa
during the Pliocene. The recently discovered human gene MYH16 is responsible for
encoding the protein myosin heavy-chain 16 that has a key role in human muscles
(Stedman, 2004). They are much weaker when this protein is absent or altered. For
example, in both humans and macaques, MYH16 is encoded by a particular gene that is
expressed in the temporalis muscle. At some point the human gene had a mutation that
strongly reduced the function of the protein. After that mutation the muscle mass
become 8 times smaller than that of the macaque. But when did this mutation occur? By
comparing the number of changes in the human gene with that in other species one can
infer that the mutation happened 2.1 to 2.7 Ma BP, exactly at the time the genus Homo
originated. Also living primates such as chimps have strong jaw muscles and in fact they
have the MYH16 gene that fully activates the function of the corresponding protein.
On the other hand, muscle size can influence bone growth. Reduction of the jaw
muscle attenuates the stress on skull bones. The larger and thinner skull of humans could
be the effect of smaller jaw muscles. In conclusion there could be a strong connection
between size of jaw muscles and size of the brain. In addition, a less massive jaw muscle
could have allowed a better coordination of the mandible function, improving speech
capabilities. The first Homo species is H. habilis that emerged around 2 - 2.5 Ma BP. At
that time, the climate was becoming drier and cooler, inducing dramatic changes of
vegetation and fauna. This is a challenging moment for hominins in Africa, who needed
to change diet and way of life in order to survive.
5.2 THE INFLUENCE OF CLIMATE
At that time not only Africa, but also the whole planet was going through dramatic
environmental changes. A progressive 50 million years of slow cooling during the
Tertiary had brought the planet into the Pleistocene, when the Arctic ice cap also
developed. Despite being referred to as ‘Ice Age’, the Pleistocene was characterized by
glacial advance, as well as recess. Long periods of glaciations were punctuated by
briefer warm periods (interglacial - lasting less than 20,000 years). Between 2.6 and 1.1
Ma BP, a full cycle of glacial advance and retreat took about 41 thousand years. During
the past 1.1 million years, the cycle lasted 100 thousand years. The paleo-temperature
record is being obtained with increasing detail measuring the oxygen isotopes in ice
cores from Antarctica and Greenland. The recent EPICA project has provided a detailed
temperature record for the last 700 thousand years (EPICA, 2004).
Homo with a larger brain and stone tools suddenly appears in the African record
following an evolutionary selection induced by environmental change. He was selected
for survival, while all the other small brained were not, except for Paranthropus
Robustus, who stayed around for a while and then also was extinct about 1 Ma BP.
16
Between 2.5 and 1.5 Ma BP, H. habilis and H. ergaster, were sharing the African
savannas with robust australopitecines. This long coexistence was made possible due to
the different strategies of adaptation for coping with increasing seasonality. Some
Australopitecines, such as the Paranthropus robustus, with their powerful chewing
muscles anchored to the characteristic sagittal crest and their large molar teeth with thick
enamel, were well adapted to eat tough fibrous plant foods. Carbon and oxygen isotope
analysis using laser ablation mass spectrometry show that the australopithecines adapted
their diet to seasonal and interannual variations [Sponheimer et al. 2006)]. H. ergaster,
with his smaller jaw muscle and smaller molar teeth, had a diet of softer food, including
fruits and meat. It is generally believed that the carnivorous diet provided the energy
needed for the increase of brain in Homo. The species Australopithecus survived until
about 1.5 -1.3 Ma BP, when the African savannahs were fully inhabited by H. ergaster,
capable of controlling fire. We know little on the details of the extinction of
australopithecines and on the role that H. ergaster and environmental change had on
their demise. Who was ergaster? Was he really the product of evolutionary events in
Africa as maintained most paleoanthropologists?
The details of the true ancestor remain uncertain. Some say habilis was the ancestor
of all species once called erectus and now called ergaster in Africa, erectus in Asia and
heidelbergensis in Europe. Other claim rudolfensis and not habilis was the ancestor of
erectus. According to Meave Leakey, keniantropus was its ancestor. In any case, erectus
suddenly appeared in Africa about 1.8 Ma BP. The best representative of this species is
the Nariokotone skeleton, 1.75-cm tall (twice the size of australopithecines), modern
body proportions, only 20% size difference between males and females. A carnivorous
creature with a brain of 900 cc and armed with advanced stone tools, he was the first
species to migrate out of Africa, conquering India, China, Sudan, eventually reaching
Europe. It is generally believed that meat was the key source of energy for the growth of
Homo’s brain that led to the bifurcation of this species from other hominids at the end of
the Pliocene.
Less orthodox viewpoints have been gaining ground recently (Dennell, 2005). It has
been suggested that the speciation event that produced Homo could have occurred in
Asia. This is based on a number of assumptions. Firstly, Australopithecus and/or habilislike hominins were living in the vast savannahs going from western Africa to China 3.5 2.5 Ma BP. There are several sites with stone tools that have chronologies older than the
date assumed for the ‘out of Africa 1’ event. ‘Taphonomic circumstances and lack of
field work’ are possible reasons explaining the absence of australopithecine fossils in
Asia. Secondly, Asia was the centre of global climate change around 2.5 Ma BP with the
arising of monsoon seasons that caused the expansion of grassland in a more dramatic
way that in Africa. Climate change was also due to the collision between Indian and
Eurasian Plaques that formed the Tibetan plateau. If climate change was the cause of the
speciation that originated Homo ergaster/erectus, one should remember that according
recent paleoclimatic studies, Asia and not Africa was at the center of the cooling event at
the Pliocene/Pleistocene boundary. Third, the more advanced Homo species that
developed in Asia as a response to the cooling event disperses back to Africa. This
would explain the sudden appearance in Africa, about 1.8 Ma BP, of H. ergaster, a
species apparently without immediate ancestors.
The main morphological effects of the evolution from australopithecines to H.
ergaster had been an increase in body size and brain volume (from 300 - 400 cc to 11001200 cc). The size of the brain is also linked to the linguistic aptitude and paleo-
17
anthropologists believe H. ergaster had already developed some capacity to
communicate in a rudimentary manner through spoken language. According to recent
hypotheses, brain size is related to jaw structure that was smaller in H. ergaster than in
earlier hominids. This was probably the effect of a diet rich in cooked meat, more
available thanks to the sophisticated stone tools with sharp blades, very efficient for
butchering large animals.
Climate variability must have been the key catalyst in the evolution of the H. sapiens
genome in Africa, possibly from H. ergaster and H. heidelbergensis, and its dispersal
into Eurasia. The climate change witnessed by modern humans in the last 100 - 200,000
years is recorded, as we said before, in polar ice cores, deep-sea sediments, corals and
varves. These terrestrial archives show with great detail the dramatic variability of
climate during this period, a feature that is common to the whole Pleistocene epoch.
Isotopic analyses provide high-resolution information on the average temperature of the
planet, dropping nearly 10 degrees below the present mean values during glacial times
and reaching higher values than the present during interglacials. The movement of
modern humans must have been seriously conditioned by the corresponding changes in
landscape, vegetation and sea level. The Sahara was a critical area influencing the
diffusion North-South of modern humans. Its occupation was favored during the warm
and wet interglacial times, while aridity produced by cold and dry glacial periods would
have made life more difficult for humans. Climate change increased the flux of people in
direction of North Africa and the Levant during glacial times, facilitating Middle
Paleolithic/Middle Stone Age cultural interactions between these areas.
Cultural and social progress during the Middle Paleolithic/ Middle Stone Age was
very slow. Rock shelters and caves became the home base for relatively large groups,
which would occasionally brake into small bands for hunting and foraging.
The first use of seafood and the grinding of plant seeds were introduced, probably during
the Last Interglacial. This diversified the diet for modern humans, introducing new
elements of flexibility, useful during their geographic spreading.
5.3 ISOTOPIC SIGNATURES OF PLANT-ANIMAL INTERACTIONS
DNA analysis in extant human populations suggests that anatomically modern humans
(AMHs) left Africa around 70 thousand years (ka) BP, reaching Australia around 50 ka
BP and Europe 40 ka BP (Wells, 2005). Archaeological sites with human presence need
to be dated in the relevant areas to confirm these predictions. A precise chronology is
also required to evaluate the impact of AMH on the ecosystem they were invading,
including their possible involvement in the extinction of the ice-age megafauna and of
‘less evolved’ human species they were encroaching (Barnosky, 2004).
As an example, Australia lost 85 % of its terrestrial animals with body mass
exceeding 44 kg during a short period of time, around 50,000 ka BP, in coincidence with
the arrival of humans. One of these animals was the large flightless bird Genyornis
newtoni. Its eggshells can be found across Australia, together those of an extant large
bird, Dromaius, the emu. The chronology related to the presence of these birds during
the late Late Pleistocene have been determined using radiocarbon dating by accelerator
mass spectrometry, amino acid racemization, and thermal ionization mass spectrometry
U-series analysis on eggshells and luminescence dating on associated sediments (Miller,
1999). More than 700 dates have been obtained, showing the continuous presence of
18
Genyornis between 100 - 50 ka BP, when it suddenly disappears from the archaeological
records. On the other hand, the eggshells of emu show their continuous presence until
the present. The diet of the two birds was reconstructed using the δ13C ratio of the
eggshells, which reflects the plants that were part of their diet. The results show that
Genyornis ate mainly C3 plants, whereas emu had a broader diet including both C3 and
C4 plants. Hence, the emu could adapt its feeding strategy to different ecological
conditions and this might have been critical for surviving through environmental
changes 50 ka BP, probably caused by the arrival of the first humans in Australia
(Miller, 2005).
6. Discussion and perspectives
6.1 WHAT HAVE WE LEARNT SO FAR?
The bicentenary of Charles Darwin’s birth in 2009 as well as the 150th anniversary of the
publication of The Origin of Species seems an appropriate time to raise questions that
Darwin was unable to do, since he was constrained to the world-view that was typical of
the 19th century. For this reason it seems timely to review, as exhaustively as possible,
the main outline of what we now have learnt so far from the evolution of life, especially
the evolution of plant-animal interactions. Within the limitations of a short review, we
have attempted to sketch a comprehensive view of evolution of life on Earth spanning
from bacteria to humans. We pointed out some insights that have already been gained
from the earliest traces of life in the Lower Archean, due to the significant combined
progress of stable isotope geochemistry and micropaleontology (Sec. 2). The ascent of
multicellular life can by now be documented convincingly (Sec. 3). There is robust
evidence for evolution of multicellular life over a billion years just before the Ediacaran
fauna (Sec. 2.1) till the origin of the primates (Sec. 5.1). This is especially convincing in
the coevolution of plants and animals that is known in a given geographical region. In
such cases evolution can be followed up in exhaustive detail, as we have done in our
region for the sake of illustration (Sec. 4). With the fast progress of technology,
especially the instrumentation of the physical sciences, we have been able to present
some microfossil evidence for both the precursors of plant and animals (Sec. 2.2). and
how the trophic relations evolved from solar energy to geothermal energy (Sec. 2.3) is
also dwelt upon. The transition from these two form of energy that are available for
living processes led to an inevitable layered segregation that even led to new niches for
anaerobes. These events left a geochemical record that testifies, convincingly, as to the
general lines of Darwinism (Sec. 2.4). New instrumentation has also enabled us to
demonstrate how plant-animal interactions have influenced the origin of humans (Sec.
5). This is relevant, since stable isotope variations have shed insights in the climate and
the diet of our ancestors. The relevant parameters are δ13C and δ 18O (Ambrose, 2006).
6.2 NEW PERSPECTIVES ON EVOLUTION
Chemical evolution has been able to sketch successfully the pathway from molecules to
the first cell. But research in astrochemistry has also outlined the pathway from
nucleosynthesis in stellar interiors to the spread of the biogenic elements (C, H, O, N, S)
19
through supernova explosions into interstellar gas, leading to the inevitable synthesis of
organic molecules that will eventually be the building blocks for the origin of life on
Earth and eventually elsewhere in the solar system and around other stars. This is the
way that evolution from the Archean, the world of Archaea and Bacteria, to the
Holocene, the world of the humans, should be viewed. Some questions that could not be
formulated during Darwin’s lifetime now begin to be forced upon us. Answers might be
within our grasp in the foreseeable future. This short review only allows us to touch on
one of them, namely, What can be learnt from the evolution of life on Earth that might
contribute to the search for life elsewhere?
This question is especially relevant for understanding the intelligibility of nature
(Chela-Flores, 2009). Many space missions are currently testing the laws of physics, the
geophysical conditions of planets and satellites of the Solar System, and searching for
evidence that evolution may have taken place elsewhere. Probing carefully the different
stages of evolution that are available to us in great detail, as we have described in the
present review, will contribute to identify useful biosignatures.
Other topics of current interest for the evolution of life are beyond the scope of our
work. The reader is referred to excellent reviews elsewhere. Amongst such questions we
should mention: Can the Modern Synthesis of Darwin’s evolution and Mendelian
genetics be enlarged to take into account present-day insights of developmental biology?
(Pennisi, 2008).
7. Acknowledgments
The authors would like to thank Dr. Romana Melis (University of Trieste) and Professor
Antonio Russo (University of Modena and Reggio Emilia) for useful suggestions
concerning plant/animal interactions.
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