The Pathology Report

Genotoxicity and Carcinogenicity of Acrylamide and its Metabolite, Glycidamide in Rodents: Two-Year Chronic Carcinogenicity Study NCTR PROTOCOL NUMBER 2150.01 (SUBCHRONIC STUDY 2150.04) PATHOLOGY REPORT PREPARED BY TOXICOLOGIC PATHOLOGY ASSOCIATES JEFFERSON, ARKANSAS FOR NATIONAL CENTER FOR TOXICOLOGICAL RESEARCH 3900 NCTR ROAD JEFFERSON, ARKANSAS 72079 November 7, 2006 Pathology Report TABLE OF CONTENTS INTRODUCTION ........................................................................................................................................... 1 EXPERIMENT DESIGN ............................................................................................................................... 1 RESULTS AND DISCUSSION ..................................................................................................................... 2 SUMMARY ..................................................................... ............................................................................... 3 UST OF TABLES Table 1 - Summary of Doses .............. ......................................................................................................... 1 LIST OF APPENDICES Appendix I Incidence Rates of Neoplasms by Anatomic Site (Pathology Report 2)........................ A-j Appendix II Incidence Rates of Non-neoplastic Lesions by Anatomic Site (Pathology Report 3) .... A-2 Appendix Ill Neoplasms by Individual Animal (Pathology Report 4 ). .............. .... ... ..... .... .... ........... .... A-3 Appendix IV Non-neoplastic Lesions by Individual Animal (Pathology Report 9) .............................. A-4 Pathology Report Genotoxlclty and Carcinogenicity of Acrylamide and Its Metabolite, Glycidamlde in Rodents: Two-Year Chronic Carcinogenicity Study NCTR PROTOCOL NUMBER 2150.01 (SUBCHRONIC STUDY 2150.04) INTRODUCTION This subchronic (13-week) toxicity study was conducted to provide information needed for dose selection for the two-year carcinogenicity study. This report by Toxicologic Pathology Associates for the National Center for Toxicological Research presents the results of pathology support for the subchronic study of B6C3F1 mice given dosed drinking water or feed as outlined in Protocol Number 2150.01 - Genotoxicity and Carcinogenicity of Acrylamide and its Metabolite, Glycidamide in Rodents: Two-Year Chronic Carcinogenicity Study. EXPERIMENT DESIGN Table 1 represents the treatment schedule from which animals were received by pathology services for necropsy, after 99 -100 days on study. Table 1 Summary of doses for subchronic (13-week) toxicity study with B6C3F1 mice administered acrylamide or g1yc I the drn I klng wa ter or feed* I Id am1 'd en Test Agent Sex Dosed Water (mM) Dosed Feed (PPM) Acrvlamide 3.52 Male 370 1.4 1 185 0.70 74 0.35 37 0 .14 18.5 0.00 0.0 Female 3.52 370 1.41 185 0.70 74 0 .35 37 0.14 18.5 0.00 0 .0 Glycldam ide Male 3.52 1.41 0 .70 0.35 0.14 0.00 Female 3.52 1.41 0.70 0.35 0 .14 0.00 - - - *Each group consisted of eight 86C3F1 mice Pathology Report At study termination, the animals were euthanized by exposure to c1:"1rbon dioxide and a complete necropsy was performed. The liver and brain were weighed as soo11 as possible after dissection. All protocol-specified tissues were examined grossly, removed, and preserved in 10% neutral buffered formalin formalin except eyes and testes which were fixed in modified Davidson's fixative. · Lesion descriptions were recorded on the IANR form (Individual Animal Necropsy Record). The protocol-designated tissues were trimmed, processed and embedded in a plastic paraffin polymer, sectioned at 4-6 microns, and stained with hematoxylin anq eosin. All tissues were examined microscopically and when applicable, non-neoplastic lesions were graded for severity as 1 (minimal), 2 (mild), 3 (moderate), or 4 (marked). RESULTS AND DISCUSSION Mortality There were four early death animals in the study, but tissue autolysis precluded determination of cause of death In all of them. One was a female from the 1.41 mM glycidamide dosed water group. The others were one male each from 18.5 ppm, 185 ppm and 370 ppm acrylamide dosed feed groups. Gross Observations Ali gross observations were recorded on the IANR form. A corresponding microscopic finding was recorded with each gross observation when possible. The on ly gross observation in the dosed water groups that was considered to be treatment related was marked dilatation of the urinary bladder in the 3.52 mM acrylamide groups. This change was evident in 100% of males and in 63% of females. This same observation was noted in one male in the 3.52 mM group treated with glycidamide. In the dosed. feed animals, 100% of the males and 88% of the females in the 370 ppm acrylamide group had a dilated urinary bladder. Ali of these animals also had a clinical observation of partial paralysis of the rear legs. Histopathology Microscopic findings are summarized by treatment groups and anatomic site in Pathology Report 2 (Neoplastic) and Pathology Report 3 (Non-neoplastic). They were also tabulated by individual animal in Pathology Report 4 (Neoplastic) and Pathology Report 9 (Non-neoplastic). Neoplastic Findings Dosed Water - One animal in the 3.52 mM glycidamlde group had a neoplastic change, which was diagnosed as a thymic lymphoma. This is not an uncommon neoplasm in B6C3F1 mice and therefore should be considered an incidental finding. Dosed Feed - There were no neoplastic lesions. . 2 Pathology Report Non-neoplastic Findings Dosed Water - Treatment related changes were observed in the following target tissues: sciatic nerve, spinal cord, skeletal muscle of the hindlimb, testes and ovaries. Target tissues were examined microscopically in progressively lower dose groups until a no-observed-adverse-effect level (NOAEL) was reached. The most significant treatment-·related change was .a radiculoneuropathy {degenerative lesion) involving the sciatic nerve and/or lumbar spinal cord {lateral funiculus) in nearly 100% of the animals treated with 3.52 mM acrylamide. This change however was not observed in the other acrylamide treated dose groups. This same lesion was noted in the sciatic nerve of one female and one male treated with 3.52 mM glycidamide. Radiculoneuropathy was characterized by nerve fiber degeneration with dilatation and vacuolization of myelin sheaths along with swollen and shrunken axons, with a severity of minimal tn mild. The neuronal degenerative changes were accompanied by muscle atrophy involving the rear legs and luminal dilatation of the urinary bladder in many of these same animals. Another significant histopathologicial finding was ovarian anestrus in 75% of the females in the 3.52 mM acrylamide group. This was not present in the lower dose groups or in animals treated with glycidamide. This change was characterized by the absence of corpora lutea in various stages of development or in regression from previous ovulations. Additionally, in males, minimal to mild depletion of the testicular germinal cell epithelium occurred in both the acrylamide and glycidamide high dose groups but not in lower dose groups. Dosed Feed - Treatment related changes were observed in the following target tissues: sciatic nerve, spinal cord , skeletal muscle of the hindlimb, testes and ovaries. Target tissues were examined until a no-observed-adverse-effect level (NOAEL) was reached . The most significant treatment-related change was radiculoneuropathy (degenerative lesion) involving the sciatic nerve of 71 % of the males and 100% of the females given 370 ppm acrylamide dosed feed. The lesion was also observed in one female in the 185 ppm dose group. The histopathologic characteristics of this lesion were very similar to those in the dosed water groups, Degenerative changes were present in the spinal cord in 29% of males of the 370 ppm dose group but were not observed in any of the female mice. The neuronal degenerative changes were accompanied by minimal to moderate muscle atrophy (rear legs) in 100% of the males and 88% of the females in the 370 ppm dose group. Luminal dilation of the urinary bladder was present in 100% of the male mice and 88% of the female mice in the 370 ppm dose group. Other significant histopathological findings were testicular degeneration in males and anestrus In females which occurred with 100% incidence in the 370 ppm dose group. These changes did not occur in any of the lower dose groups. The remaining non-neoplastic findings were considered incidental background changes commonly associated with 86C3F1 mice. SUMMARY This study establishes in B6C3F1 mice a no-observed-adverse-effect level (NOAEL) of 1.41 mM for acrylamide and glycidamide administered in the drinking water and 185 ppm acrylamide In dosed feed, since the high dose of 3.52 mM (water) and 370 ppm (feed) resulted in radiculoneuropathy, testicular germinal cell depletion, anestrus and muscle atrophy in a high percentage of exposed animals. Greg Olson, DVM, Ph.D. 3 Pathology Report Appendix I - Incidence Rates of Neoplams by Anatomic Site (Pathology Report 2) A-1 TOMS No. 2C213 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-0AY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR . Pathologist: OLSON, G. C Number: C20313 Lock Date: Not Entered. Cage Range: ALL Date Range: ALL Reasons For Removal: ALL Removal Date Range: ALL Treatment Groups: Include ALL Date Report Reqsted: 06/29/2006 Time Report Reqsted : 08:15:54 First Dose M/F: 07112/04 I 07112/04 Lab: NCTR TOMS No. 20313- 02 Test Type: 90-DAY P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) . ACRYLAMIDE Route: DOSED WATER C57BU6N XC3H/HEN MTV-NCTR MICE MALE Fi rst Dose M/F: 07/12104 / 07/12104 CAS Number : 79-06-1 Species/Strain: MICE/B6C3F1/NCTR Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 Lab: NCTR Pathologist: OLSON, G . Control Water M 0.14 Acryl M 0.35 Acryl M 0.70 Acryl M 1.41 Acryl M 8 8 8 8 8 8 8 8 8 2 8 8 8 8 (8) (0) (0) (0) (0) (8) (0) (0) (0) (0) (8) (8) (0) (0) (2) (0) (0) (0) (O) (8) Disposition Summary Animals Initially in Study Early·Deaths Survivo rs Terminal Sacrifice Animals Examined Microscopically ALIMENTARY SYSTEM Liver CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM Adrenal Cortex GENERAL BODY SYSTEM . None GENITAL SYSTEM Preputial Gland Testes HEMATOPOIETIC SYSTEM None INTEGUMENTARY SYSTEM None I a - Number of animals examined microscopically al site and number of animals wilh lesion Page2 TOMS No. 20313 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY ACRYLAMIDE CAS Number: 79-06-1 Route: DOSED WATER Species/Strain: MICE/B6C3F1 /NCTR C57Bl.i6N XC3H/HEN MTV-NCTR MICE Date Report Reqsled: 06129/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07112/04 / 07112/04 Pathologist: OLSON, G. Lab: NCTR Control Water M 0.14 Acryl M 0.35 Acryl M 0.70 Acryl M 1.41 Acryl M (8) (0) (0) (8) (8) (8) (0) (0) (0) {8) (8) (8) (0) (0) (8) (8) (0) (0) (0) (0) MALE MUSCULOSKELETAL SYSTEM Skeletal Muscle NERVOUS SYSTEM Peripheral Nerve Spinal Cord, Lumbar ' RESPIRATORY SYSTEM None SPECIAL SENSES ~YSTEM None URINARY SYSTEM Urinary Bladder a - Number of animals examined microscopically at site and number of animals with lesion Page3 TOMS No. 20313 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Pathologist: OLSON, G. Species/Strain: MICEl86C3F1 /NCTR C57BU6N XC3H/HEN MTV-NCTR MICE MALE 3.52 Acryl M Disposition Summary Animals Initially in Study Early Deaths Survivors Terminal Sacrifice Animals Examined Microscopically 8 8 8 ALIMENTARY SYSTEM Liver (8) CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM Adrenal Cortex (8) GENERAL BODY SYSTEM None GENITAL SYSTEM Preput1al Gland Testes (8) (8) HEMATOPOIETIC SYSTEM None INTEGUMENTARY SYSTEM None a - Number of animals examined microscopically at site and number of animals with lesion Page4 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 0711 2/04 Lab: NCTR TOMS No. 20313- 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR C57BU6N XC3H/HEN MTV-NCTR MICE Pathologist: OLSON, G. 3.52Acryl M MALE MUSCULOSKELETALSYSTEM SKzic~;:I Muscle (8) NERVOUS SYSTEM Peripheral Nerve Spinal Cord, Lumbar (8) (8) RESPIRATORY SYSTEM None SPECIAL SENSES SYSTEM None URINARY SYSTEM Urinary Bladder (8) a - Number of animals examined microscopically at site and number of animals with lesion Page 5 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose MJF: 07/12/04 / 07/ 12104 Lab:NCTR TOMS No. 20313 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY Route: DOSED WATER ACRYLAMIDE C::AS Number: 79-06-1 · Pathologist: OLSON, G. Species/Strain: MICE/B6C3F1 /NCTR C57BU6N XC3HJHEN MTV-NCTR MICE MALE Control Water M 0.14 Acryl M Tumor Summary for Males Total Animals with Primary Neoplasms (b) Total Primary Neoplasms Total Animals with Benign Neoplasms Total Benign Neoplasms Total Animals with Malignant Neoplasms Total Malignant Neoplasms Total Animals w ith Metastatic Neoplasms Total Metastatic Neoplasms Total Animals with Malignant Neoplasms Uncertain Primary Site Total Animals with Neoplasms Uncertain-Benign or Malignant Total Uncertain Neoplasms a - Number of animals examined microscopically at site and number of animals with lesion b - Primary tumors: all tumors except metastatic tumors Page6 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12104 I 07112104 Lab: NCTR 0.35Acryl M 0.70Acryl M 1.41 Acryl M TOMS No. 20313 - 02 Test Type: 90-DAY Route; DOSED WATER Species/S1rain: MICE/86C3F1/NCTA C57BU6N XC3H/HEN MTV-NCTR MICE P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) ACRYLAMIDE CAS Number: 79-06-1 Pathologist: OLSON, G. 3.52 Acryl M MALE Tumor Summary for Males Total Animals with Primary Neoplasms (b) Total Primary Neoplasms Total Animals with Benign Neoplasms Total Benign Neoplasms Total Animals with Malignant Neoplasr.1s Total Malignant Neoplasms Total Animals with Metastatic Neoplasms Total Metastatic Neoplasms Total Animals with Malignant Neoplasms Uncertain Primary Site Total Animals with Neoplasms Uncertain-Benign or Malignant Total Uncertain Neoplasms *** END OF MALE ... a - Number of animals examined microscopically at site and number ol animals with lesion b - Primary tumors: all tumors except metastatic tumors Page7 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12104 / 07112104 Lab: NCTR TOMS No. 20313 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Date Report Reqsted: 06/29/2006 ACRYLAMIDE Time Report Reqsted: 08:15:54 First Dose MJF: 07112/04 / 07112/04 Test Type: 90-DAY Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR C57BU6N XC3H/HEN MTV-NCTR MICE Pathologist: OLSON, G. Lab: NCTR Control Water F 0.14 Acryl F 0.35 Acryl F 0.70 Acryl F 1.41 Acryl F 8 8 8 8 8 8 8 8 8 1 8 8 8 8 {8) {O) (1) (O) (0) (8) (0) (0) (0) (0) (8) (0) {O) (0) (0) (8) (0) (1) (0) (8) (8) (0) (0) (1) (0) FEMALE Disposition Summary Anlmals Initially in Study Early Deaths Survivors Terminal Sacrifice Animals Examined Microscopically ALIMENTARY SYSTEM Liver CARDIOVASCULAR SYSTEM Heart ENDOCRINE SYSTEM Adrenal Cortex GENERAL BODY SYSTEM None GENITAL SYSTEM Ovary HEMATOPOIETIC SYSTEM Spleen INTEGUMENTARY SYSTEM None a - N• • mb~r of animals examined microscopically at site and number of animals with lesion Page 8 TOMS No. 20313 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-0AY ACAYLAMIDE CAS Number: 79-06-1 Route: DOSED WATER Species/Strain: MICE/86C3F1/NCTA C57BU6N XC3HJHEN MTV-NCTR MICE FEMALE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07112/04 I 07/12104 Pathologist: OLSON, G. Lab: NCTR Control Water F 0.14Acryl F 0.35 Acryl F 0.70 Acryl F 1.41 Acryl F (8) (0) (0) (8) (8} (8) (0) (0) (8) (0) (0) (8) (0) (8) (8) (0) (0) (1) (O) (8) (0) (0) (O} (0) MUSCULOSKELETALSYSTEM Skeletal Muscle NERVOUS SYT~M Peripheral Nerve Spinal Cord, Lumbar (8) RESPIRATORY SYSTEM None SPECIAL SENSES SYSTEM Eye URINARY SYSTEM Urinary Bladder a - Number of animals examined microscopically at site and number of animals with lesion Page9 TOMS No. 20313- 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTR C57BU6N XC3H/HEN MlV-NCTR MICE Pathologist: OLSON, G. 3.52 Acryl F FEMALE Disposition Summary Animals Initially in Study Early Deaths Survivors Terminal Sacrifice Animals Examined Microscopically 8 8 8 ALIMENTARY SYSTEM Liver (8) CARDIOVASCULAR SYSTEM Heart (8) ENDOCRINE SYSTEM Adrenal Cortex (8) GENERAL BODY SYSTEM None GENITAL SYSTEM Oval)' (8) HEMATOPOIETIC SYSTEM Spleen (8) INTEGUMENTARY SYSTEM None a - Number of animals examined microscopically at site and number of animals with lesion Page 10 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:1 5:54 First Dose M/F: 07/12104 / 07/12104 lab:NCTR TOMS No. 20313 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY ACRYLAMIDE Route: DOSED WAT ER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR C57BU6N XC3H/HEN MTV-NCTR MICE · Pathologist: OLSON, G. 3.52 Acryl F FEMALE MUSCULOSKELETALSYSTEM Skeletal Muscle (8) NERVOUS SYSTEM Peripheral Nerve Spinal Cord, Lumbar (8) (8) RESPIRATORY SYSTEM None SPECIAL SENSES SYSTEM Eye (8) URINARY SYSTEM Urinary Bladder (8) a - Number of animals examined microscopically at site and number of animals with lesion Page 11 Date Re.p ort Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 I 07112104 Lab: NCTR TDll'l'S No. 20313 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY Route: DOSED WATER ACRYLAMIDE CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR C578U6N XC3H/HEN MTV-NCTR MICE FEMA:.. E First Dose M/F: 07/12/04/07/12/04 Pathologist: OLSON, G. Control Water F 0.14 Acryl F Tumor Summary for Females Total Animals with Primary Neoplasms (b) Total Primary Neoplasms Total Animals with Benign Neoplasms Total Benign Neoplasms Total Animals with Malignant Neoplasms Total Malignant Neoplasms Total Animals with Metastatlc Neoplasms Total Metastatic Neoplasms Total Animals with Malignant Neoplasms Uncertain Primary Site Total Animals with Neoplasms Uncertain-Benign or Malignant Total Uncertain Neoplasms a - N umber of animals examined microscopically at site and number of animals wilh lesion b - Primary tumors: all tumors except metastatic tumors Page 12 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 Lab: NCTR 0.35 Acryl F 0..70 Acryl F 1.41 Acryl F TOMS No. 20313 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-0AY A9AYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR C57BU 6N XC3H/HEN MTV-NCTR MICE FEMALE Pathologist: OLSON, G. 3.52 Acryl F Tumor Summary for Females Total Animals with Primary Neoplasms (b) Total Primary Neoplasms Total Animals with Benign Neoplasms Total Benign Neoplasms Total Animals with Malignant Neoplasms Total Malignant Neoplasms Total Anlmals with Metastatic Neoplasms Total Metastatic Neoplasms Total Animals with Malignant Neoplasms Uncertain Primary Site Total Animals with Neoplasms Uncertain-Benign or Malignant Total Uncertain Neoplasms .- END OF REPORT ... a - Number of animals examined microscopically at site and number of animals with lesion b - Primary tumors: all tumors except metastatic tumors Pa ge i3 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 first Dose M/F: 07112/04 / 07/12/04 Lab: NCTA TDMS No. 20314 · 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) GLYCIDAMIDE Test Type: 90-DAY Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694·00·8 first Dose M/F: 07/26/04 / 07/26/04 Species/S1rain: MICE/86C3F1/NCTR Pathologist: OLSON, G. lab: NCTR C Number: C20314 Lock Date: Not Entered. Cage Range: ALL Date Range: ALL Reaso!1s For Removal: ALL Removal Date Range: ALL Treatment Groups: Include ALL TOMS No. 20314 • 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-0AY GLYCIDAMIOE Route: DOSED WATER CAS Number: 5694-00-8 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. C57BU6N XC3HMEN MTV-NCTR MICE MALE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07126104 Lab: NCTR Control Water M 0.14 Glycid M 0.35 Glycid M 0.70 Glycld M 1.41 Glycid M 8 8 8 8 8 8 8 2 8 2 8 8 8 8 (8) (0) (0) (0) (0) (7) (0) (0) (O) (0) (8) (1) (1) (0) (0) (8) (0) (0) (0) (8) (8) (0) (1) (0) (0) Disposition Summary Animals Initially in Study Early Deaths Survivors Terminal Sacrifice Animals Examined Microscopically ALIMENTARY SYSTEM Liver CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM Adrenal Cortex GENERAL BODY SYSTEM None GENITAL SYSTEM Preputial Gland Testes HEMATOPOIETIC SYSTEM None :NTEGU~ .1 ENTARY Skin SYSTEM a - Number of animals examined microscopically at site and number of animals with lesion Page2 TOMS No. 20314 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-0AY GLYCIOAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07126104 Species/Strain: MICE/B6C3F1/NCTR .Pathologist: OLSON, G. Lab: NCTR C57BU6N XC3H/HEN MTV-NCTR MICE MALE Control Water M . 0.14 Glycid M 0.35 Glycid M 0.70 Glycid M 1.41 Glycid M MUSCULOSKELETALSYSTEM None NERVOUS SYSTEM Peripheral Nerve (8) (0) (0) (0) (0) (8) (0) (0) (0) (0) (8) (0) (0) (0) (0) RESPIRATORY SYSTEM lung SPECIAL SENSES SYSTEM None URINARY SYSTEM Urinary Bladder a - Number of animals examined microscopically at site and number of animals with lesion Page3 TDMS No. 20314- 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07126/04 / 07/26/04 Species/Strain: MICE/B6C3F1/NCTA Pathologist: OLSON, G. Lab: NCTR C57BU6N XC3H/HEN MTV-NCTR MICE 3.52 Glycid M MALE Disposition Summary Animals Initially in Study Early Deaths Survivors Terminal Sacrifice Animals Examined Microscopically 8 8 8 ALIMENTARY SYSTEM Liver (8) CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM Adrenal Cortex (8) GENERAL BODY SYSTEM None GENITAL SYSTEM Preputial Gland Testes (8) (8) HEMATOPOIETIC SYSTEM None INTEGUMENTARY SYSTEM Skin . (8) a - Number of animals examined microscopically at site and number ol animals with lesion Page4 TOMS No. 20314 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY G~ YCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07/26/04 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR C57BLJ6N XC3H/HEN MTV-NCTR MICE MALE 3.52 Glycid M MUSCULOSKELETALSYSTEM None NERVOUS SYSTEM Peripheral Nerve (8) RESPIRATORY SYSTEM Lung (8) SPECIAL SENSES SYSTEM None URINARY SYSTEM Urinary Bladder (8) a - Number of animals examined microscopically at site and number of animals with lesion Pages TOMS No. 20314 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose MIF: 07/26/04 / 07126104 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON. G. Lab: NCTR C57BU6N XC3HJHEN MTV-NCTR MICE Control Water M 0.14 Glycid M MALE Tumor Summary for Males Total Animals with Primary Neoplasms {b) Total Primary Neoplasms Total Animals with Benign Neoplasms Total Benign Neoplasms Total Animals with Malignant Neoplasms Total Malignant Neoplasms Total Animals with Metastatic Neoplasms Total Metastatic Neoplasms Total Animals with Malignant Neoplasms Uncertain Primary Site Total Animals with Neoplasms Uncertain-Benign or Malignant Total Uncertain Neoplasms a - Number of animals examined microscopically at site and number of animals with lesion b - Primary tumors: all tumors except metastatic tumors Page6 0.35 Glycid M 0.70 Glycid M 1.41 Glycid M TDMS No. 20314 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY Route: DOSED WATER GLYCIDAMIDE CAS Number: 5694-00-8 Species/Strain: MICEJB6C3F1/NCTR Pathologist: OLSON, G. C57BU6N XC3H/HEN MTV-NCTR MICE 3.52 Glycid M MALE Tumor Summary for Males Total Animals w ith Primary Neoplasms (b) Total Primary Neoplasms Total Animals with Benign Neoplasms Total Benign Neoplasms Total Animals with Malignant Neoplasms Total Mal ignant Neoplasms Total Animals with Metastatic Neoplasms Total Metastatic Neoplasms Total Animals with Malignant Neoplasms Uncertain Primary Site Total Ani mals with Neoplasms Uncertain-Benign or Malignant T.;~I Uncertain Neoplasms ... END OF MALE *** a - Number of animals examined microscopically at site and number of animals with lesion b - Primary tumors: all tumors except metastatic tumors Page ? Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07/26/04 Lab: NCTR TOMS No. 20314- 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY Route: DOSED WATER GLYCIDAMIDE CAS Number: 5694-00-8 . Pathologist: OLSON, G. Species/Strain: MICE/86C3F1/NCTR C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07126104 I 07126104 lab: NCTR Control Water F 0.14 Glycid F 0.35 Glycid F 0.70 Glycid F 1.41 Glycid F 8 8 8 8 8 8 8 2 7 (0) (1) (0) Disposition Summary Animals Initially in Study Early Deaths Natural Death Survivors Tenninal Sacrifice Animals Examined Microscopically 8 8 8 1 (0) (0) (0) (0) (0) (8) (8) (0) (0) (0) (0) (1) (7) (0) (0) (0) (1) (8) (1) (0) (1} (1) (8) (0) (O) (0) (0) 1 ALIMENTARY SYSTEM Mesentery Pancreas Salivary Glands CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM Adrenal Cortex GENERAL BODY SYSTEM None GENITAL SYSTEM Ovary HEMATOPOIETIC SYSTEM Thymus Lymphoma Malignant a - Number of animals examined microscopically at site and number of animals with lesion Page8 TOMS No. 20314 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 GLYCIDAMIDE Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07126104 Species/Strain: MICE/86C3F1/NCTA Pathologist: OLSON, G. Lab: NGTA C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE Control Water F 0.14 Glycid F 0.35 Glycid F 0.70 Glycid F 1.41 Glycid F (8) (0) (0) (0) (0) ·ca> •(1) •(2) "(1) INTEGUMENTARY SYSTEM None -MUSCULOSKELETAL SYST EM None -NERVOUS SYST EM Peripheral Nerve RESPIRATORY SYST EM None SPECIAL SENSES SYSTEM None URINARY SYST EM None 4 SYSTEMIC LESIONS Multiple Organs Lymphoma Malignant a - Number of animals examined microscopically at site and number of animals with lesion • Number of animals with any tissue examined microscopically Page 9 TOMS No. 20314 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number. 5694-00-8 First Dose M/F: 07/26/04 I 07126104 Species/Strain: MICEJ86C3F1/NCTR Pathologist; OLSON, G. Lab: NCTR C57BU6N XC3H/HEN MTV-NCTR MICE 3.52 Glycid F FEMALE Disposition Summary Animals Initially in Study Early Deaths Natural Death Survivors l e1 .ilinal Sacrifice Animals Examined Microscopically 8 8 8 ALIMENTARY SYSTEM Mesentery Pancreas Salivary Glands (0) (8) (8) CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM Adrenal Cortex (7) GENERAL BODY SYSTEM None GENITAL SYSTEM Ovary (8) HEMATOPOIETIC SYSTEM Thymus l ymphoma Malignant (8) 1 (13%) a - Number of animals examined microscopically at site and number of animals with lesion • Number of animals with any tissue examined microscopically Page 10 TOMS No. 20314 - 02 TestType: 90-DAY P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose MJF: 07/26/04 I 07126104 Species/Strain: MICEJB6C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE 3.52 Glycid F INTEGUMENTARY SYSTEM None MUSCULOSKELETAL SYSTEM None NERVOUS SYSTEM Peripheral Nerve (8) RESPIRATORY SYSTEM None SPECIAL SENSES SYSTEM None URINARY SYSTEM None SYSTEMIC LESIONS Multiple Organs Lymphoma Malignant ·(a) 1 (13%) a - Number of animals examined microscopically at site and number of animals with lesion • Number of animals with any tissue examined microscopically Page 11 TOMS No. 20314 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 GLYCIDAMIDE Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07126104 Species/Strain: MICEJB6C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR C578U6N XC3HIHEN MTV-NCTR MICE FEMALE Control Water F 0.14 Glycid F Tumor Summary for Females Total Anjmals with Primary Neoplasms (b) Total Primary Neoplasms Total Animals with Benign Neoplasms Total Benign Neoplasms Total Animals with Malignant Neoplasms Totrl Malignant Neoplasms Total Animals with Metastatic Neoplasms Total Metastatic Neoplasms Total Animals with Malignant Neoplasms Uncertain Primary Site Total Animals with Neoplasms Uncertain-Benign or Malignant Total Uncertain Neoplasms a - Number of animals examined microscopically at site and number of animals with lesion b - Pnmary tumors: all tumors except metastatic tumors Page 12 0.35 Glycid F 0.70 Glycid F 1.41 Glycid F TOMS No. 2031 4 - 02 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY GLYCIDAMIDE Route: DOSED WATER CAS Number: 5694-00-8 . · Pathologist: OLSON, G. Species/Strain: MICE/86C3F1/NCTR C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE 3.52 Glycid F Tumor Summary for Females Total Animals with Primary Neoplasms (b) Total Primary Neoplasms Total Animals with Benign Neoplasms Total Benign Neoplasms Tola! Animals with Malignant Neoplasms Total Malignant Neoplasms Total Animals with Metastatic Neoplasms Total Metastatic Neoplasms Total Animals with Malignant Neoplasms Uncertain Pri mary Site Total Animals with Neoplasms Uncertain-Benign or Malignant Total Uncertain Neoplasms • •• END OF REPORT ... a - N umber of animals examined microscopically at site and number of animals with lesion b - Primary tumors: all tumors except metastatic tumors Page 13 Date Report Reqsted: 06f29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07/26/04 Lab: NCTA TOMS No. 20313 - 04 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) ACRYLAMIDE Test Type: 90-DAY Route: DOSED FEED Species/Strain: MICE/86C3F1/NCTR CAS Number: 79-06-1 Pathologist: OLSON, G. C Number: C20313B Lock Date: Not Entered. Cage Range: ALL Date Range: ALL Reasons For Removal: ALL Removal Date Range: ALL Treatment Groups: Include ALL Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08102/04 / 08/02/04 Lab: NCTR TOMS No. 20313 - 04 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/S!!':iin: MICE/86C3F1 /NCTR C57BU6N XC3H/HEN MTV-NCTR MICE Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08102/04 I 08/02/04 Pathologist: OLSON, G. Lab: NCTR Control Feed M 18.5 PPM Aery! M 37 PPM Aery! M 74 PPM Acryl M 185 PPM Acryl M 8 8 8 8 8 8 7 1 8 8 2 8 (8) (0) (0) (0) (0) (0) (0) (1) (B) (8) (0) (0) (1) (1) MALE Disposition Summary Animals Initially in Study Early Deaths Accidently Killed Natural Death Survivors Terminal Sacrifice Animals Examined Microscopically 8 7 ALIMENTARY SYSTEM None CARD IOVASCULAR SYSTEM None ENDOCRINE SYSTEM None GENERAL BODY SYSTEM None GENITAL SYSTEM Epididymis Testes (8) HEMATOPOIETIC SYSTEM Spleen a - Number of animals examined microscopical[y at site and number of animals with lesion Page 2 TOMS No. 20313 - 04 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-0AY ACRYLAMIDE Route: DOSED FEED CAS Number: 79--06-1 Species/Strain: MICE/B6C3F1/NCTA C57BU6N XC3H/HEN MTV-NCTR MICE MALE Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 Pathologist: OLSON, G. Lab: NCTR Control Feed M 18.5 PPM Acryl M 37 PPM Acryl M 74 PPM AcryL M 185 PPM Acryl M (8) (0) (0) (0) (8) (8) (8) (0) (0) (0) (O) (0) (0) (8) (8) (0) (0) (0) (1) INTEGU M ENTARY SYSTEM None -MUSCULOSK ELETAL SYSTEM Skeletal Muscle N ERVOUS SYSTEM Peripheral Nerve Spinal Cord, Lumbar (8) R ESPIRATORY SYSTEM None SP ECIAL S ENSES SY STEM None URINARY SYSTEM Urinary Bladder a - Number of animals examined microscopically at site and number of animals with lesion Page 3 TOMS No. 20313- 04 Test Type: 90-DAY P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Route: DOSED FEED CAS Number: 79-06-1 Pathologist: OLSON, G. ACRYLAMIDE Species/Strain: MICE/86C3F1 /NCTR CS7BU~N XC3H/HEN MTV-NCTR MICE 370 PPM Acryl M MALE Disposition Summary Animals Initially in Study Early Deaths Accidently Killed Natural Death Survivors Terminal Sacrifice Animals Examined Microscopically 8 7 8 ALIMENTARY SYSTEM None CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM None GENERAL BODY SYSTEM None GENITAL SYSTEM Epididymis Testes (7) (7) HEMATOPOIETIC SYSTEM Spleen (7) a - Number of animals examined microscopically at site and number of animals with lesion Page4 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose MJF: 08/02/04 I 08102104 Lab: NCTR TOMS No. 20313 - 04 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1 /NCTA C57BU6N XC3H/HEN MTV-NCTR MICE MALE Pathologist: OLSON, G. 370 PPM Acryl M INTEGUMENTARY SYSTEM None MUSCULOSKELETAL SYSTEM Skeletal Muscle (7) NERVOUS SYSTEM Peripheral Nerve Spinal Cord, Lumbar (7) (7) RESPIRATORY SYSTEM None SPECIAL SENSES SYSTEM None URINARY SYSTEM Urinary Bladder (7) a - Number of animals examined microscopically at site and number ot animals wrth lesion Page 5 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 I 08102104 Lab: NCTR TOMS No. 20313 - 04 Test Type: 90-DAY Route; DOSED FEED P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) ACRYLAMIDE CAS Number; 79-06-1 Pathologist; OLSON, G. Species/Strain; MICE/B6C3F1 INCTA C578U6N XCJH/HEN MTV-NCTR MICE MALE Control Feed M 18.5 PPM Aery! M Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02104 Lab: NCTR 37 PPM Aery! M 74 PPM Acryl M 185 PPM Aery! M Tumor Summary for Males Total Animals with Primary Neoplasms (b) Total Primary Neoplasms Total Animals with Benign Neoplasms Total Benign Neoplasms Total Animals with Malignant Neoplasms Total Malignant Neoplasms Total Animals with Metastatic Neoplasms Total Metastatic Neoplasms Total Animals with Malignant Neoplasms Uncertain Primary Site Total Animals with Neoplasms Uncertain-Benign or Malignant Total Uncertain Neoplasms ; a - Number of animals examineu m1croscopica1ly at site and number of animals with lesion b - Primary tumors: all tumors except metastatic tumors Page6 TOMS No. 20313 - 04 Test Type: 90-DAY Route: DOSED FEED P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) AC.RYLAMIDE CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR C57BU6N XC3HIHEN MTV-NCTR MICE MALE Pathologist: OLSON, G. 370 PPM Acryl M Tumor Summary for Males Total Animals with Primary Neoplasms (b) Total Primary Neoplasms Total Animals with Benign Neoplasms Total Benign Neoplasms Total Animals with Malignant Neoplasms Total Malignant Neoplasms Total Animals with Metastatic Neoplasms Total Metastatic Neoplasms Total Animals with Malignant Neoplasms Uncertain Primary Site Total Animals with Neoplasms Uncertain-Benign or Malignant Total Uncertain Neoplasms *** END OF MALE *** a - Number of animals examined microscopically at site and number of animals with lesion b - Primary tumors: all-tumors except metastatic tumors Page7 Date Report Reqsted: 07/31 /2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 Lab: NCTR TDMS No. 2031 3- 04 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY ACAYLAMIDE Rout e: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR C57BL16N XC3H/HEN MTV-NCTR MICE FEMALE Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08102104 Pathologist: OLSON, G. Lab: NCTR Control Feed F 18.5 PPM Acryl F 37 PPM Acryl F 74 PPM Acryl F 185 PPM Acryl F 8 8 8 8 8 8 8 8 8 8 Disposition Summary Animals Initially in Study Early Deaths Survivors Terminal Sacrifice Animals Examined Microscopically 8 8 ALIMENTARY SYSTEM None CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM Adrenal Cortex (8) (0) (0) (0) (0) (8) (0) (0) (0) (8) (8) (8) (8) (0) (0) (0) (0) (0) (0) (0) (0) (0) (0) GENERAL BODY SYSTEM None GENITAL SYSTEM Ovary HEMATOPOIETIC SYSTEM Lymph Node, Mandibular Lymph Node, Mesenteric Spleen (0) INTEGUMENTARY SYSTEM a • Number ol animals examined microscopically at site and number of animals with lesion Page 8 (0) TOMS No. 20313 - 04 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR C57Bl/6N XC3H/HEN MTV-NCTR MICE FEMALE Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08102104 Pathologi st: OLSON, G. lab: NCTR Control Feed F 18.5 PPM Acryl F 37 PPM Acryl F 74 PPM Acryl F 185 PPM Acryl F (8) (0) (0) (0) (8) (8) (0) (0) (0) (8) (8) (0) (0) (0) (0) None -MUSCULOSKELETAL SYSTEM Skeletal Muscle NERVOUS SYSTEM Peripheral Nerve RESPIRATORY SYSTEM None SPECIAL SENSES SYSTEM None URINARY SYSTEM Urinary Bladder a - Number of animals examined microscopically at site and number of animals with leslon Page9 TOMS No. 20313- 04 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR C57BU6N XC3H/HEN MTV-NCTA MICE FEMALE Pathologist: OLSON, G. 370 PPM Acryl F Disposition Summary Animals Initially in Study Early Deaths Survivors Terminal Sacrifice Animals Examined Microscopically 8 8 8 ALIMENTARY SYSTEM None CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM Adi;: ;,~ ; Cortex (8) GENERAL BODY SYSTEM None GENITAL SYSTEM Ovary (8) HEMATOPOIETIC SYSTEM Lymph Node, Mandibular Lymph Node, Mesenteric Spleen (8) (8) (8) INTEGUMENTARY SYSTEM a - Number of animals examined microscopically at site and number of animals with lesion Page 10 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02104 Lab: NCTR TOMS No. 20313- 04 Test Type: 90-DAY Route: DOSED FEED P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) ACRYLAMIDE CAS Number: 79-06-1 Species/Strain: MICE/B6C3Ft /NCTR C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE Pathologist: OLSON, G. 370 PPM Acryl F None MUSCULOSKELETALSYSTEM Skeletal Muscle (8) NERVOUS SYSTEM Peripheral Nerve (7) RESPIRATORY SYSTEM None SPECIAL SENSES SYSTEM None URINARY SYSTEM Urinary Bladder (8) a - Number of animals examined microscopically at site and number of animals with lesion Page 11 Date Report Reqsted: 07/31 /2006 Time Report Reqsted: 12.:29:56 First Dose M/F: 08/02/04 / 08102/04 Lab: NCTR TOMS No. 20313 - 04 P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08102/04 Pathologist: OLSON, G. Species/Strain: MICEJB6C3F 1/NCTR Control Feed F 18.5 PPM Acryl F Tumor Summary for Females Total Animals with Primary Neoplasms (b) Total Primary Neoplasms Total Animals with Benign Neoplasms Total Benign Neoplasms Total Animals with Malignant Neoplasms Total Malignant Neoplasms Total Animals with Metastatic Neoplasms Total Metastatic Neoplasms Total Animals with Malignant Neoplasms Uncertain Primary Sile Total Animals with Neoplasms Uncertain-Benign or Malignant Total Uncertain Neoplasms a • Number of animals examineu m1croscopically at Site and number of animals wilh lesion b - Primary tumors: alf tumors except metastatic tumors Page 12 Date Report Reqsted: 07/31 /2006 Lab: NCTR 37 PPM Acryl F 74 PPM Acryl F 185 PPM Acryl F TOMS No. 20313 · 04 Test Type: 90·DAY Route: DOSED FEED P02: INCIDENCE RATES OF NEOPLASMS BY ANATOMIC SITE (a) ACRYLAMIDE CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1JNCTR C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE Pathologist: OLSON, G. 370 PPM Acryl F Tumor Summary for Females Totai Ar.lmals with Primary Neoplasms (b) Total Primary Neoplasms Total Animals with Benign Neoplasms Total Benign Neoplasms Total Animals with Malignant Neoplasms Total Malignant Neoplasms Total Animals with Metastatic Neoplasms Total Metastatic Neoplasms Total Animals with Malignant Neoplasms Uncertain Primary Site Total Animals with Neoplasms Uncerla i n~Beig or Malignant Total Uncertain Neoplasms °"END OF REPORT··· a - Number of animals examined microscop1cally at site and number of animals with lesion b - Primary tumors: all tumors except metastatic tumors Page 13 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02104 / 08/02/04 Lab: NCTR Pathology Report Appendix II Incidence Rates of Non-neoplastic Lesions by Anatomic Site (Pathology Report 3) A-2 TOMS No. 20313- 02 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-DAY ACRYLAMIDE Date Report Reqsted: 06129/2006 Time Report Aeqstecl: 08:15:54 Route: DOSED WATER . CAS Number: 79-06-1 First Dose M/F: 07/12104 / 07112104 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR C Number: C20313 Lock Date: Not Entered. Cage Range: ALL Date Range: ALL Reasons For Removal: ALL Removal Date Range: ALL Treatm~n Groups: Include ALL TOMS No. 20313 - 02 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Pathologist: OLSON, G. Species/Strain: MICE/86C3F1/NCTR C57BU6N XC3H/HEN MTV-NCTR MICE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07/ 12/04 Lab: NCTR Control Water M o.'14 Acryl M 0.35Acryl M 0.70 Acryl M 1.41 Acryl M 8 8 8 8 8 8 8 8 8 2 8 8 8 8 (8) (0) (0) (0) (0) (0) (0) (0) (0) (0) (2) 1 (50%) (0) (0) (0) (0) (0) (8) MALE Disposition Summary Ani mals Initially in Study Early Deaths Survivors Terminal Sacrifice Animals Examined Microscopically ALIMENTARY SYSTEM Liver Inflammation, Chronic Active Tension Lipidosis 1 (13%} CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM Adrenal Cortex Subcapsular, Hyperplasia ~ENRAL (8) 3 (38%) BODY SYSTEM None GENITAL SYSTEM Preputial Gland Cyst Testes Germinal Epithelium, Degeneration (8) 2 (25%) (8) -HEMATOPOIETIC SYSTEM None a - Number of animals examined microscopically at site and number of animals with lesion Page2 TOMS No. 20313 - 02 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-0AY ACRYLAMIOE CAS Number: 79-06-1 Route: DOSED WATER Species/Strain: MICEJ86C3F1 /NCTR C57BU6N XC3H/HEN MTV-NCTR MICE MALE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12104 / 07/12104 Lab: NCTR Pathologist: OLSON, G. Control Water M 0.14 Acryl M 0.35Acryl M 0.70Acryl M 1.41 Acryl M (8) (0) (0) (8) (8) (8) (0) (0) (8) (8) (8) (0) (0) (0) (8) (8) (0) (0) (0) (0) INTEGUMENTARY SYSTEM None MUSCULOSKELETALSYSTEM Skeletal Muscle Atrophy NERVOUS SYSTEM Peripheral Nerve Axon, Degeneration Spinal Cord, Lumbar Axon, Degeneration RESPIRATORY SYSTEM None SPECIAL SENSES SYSTEM None URINARY SYSTEM Urinary Bladder Lumen, Dilatation a - Number of animals examined microscopically at site and number of animals with lesion Page3 TOMS No. 20313 - 02 Test f yp..: 90-DAY P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Route: DOSED WATER CAS Number: 79-06-1 ACRYLAMIDE Species/Strain: MICE/B6C3F1/NCTR C57BV6N XC3H/HEN MTV-NCTR MICE MALE Pathologist: OLSON, G. 3.52Acryl M Disposition Summary Animals Initially In Study Early Deaths Survivors Terminal Sacrifice Animals Examined Microscopically 8 8 8 ALIMENTARY SYSTEM Liver Inflammation, Chronic Active Tension Lipidosis (8) 1 (13%) 1 (13%) CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM Adrenal Cortex Subcapsular, Hyperplasia (8) GENERAL BODY SYSTEM None GENITAL SYSTEM Preputial Gland Cyst Testes Germinal Epithelium, Degeneration (8) (8) 6 (75%) HEMATOPOIETIC SYSTEM None a - Number of animals examined microscopically at site and number of animals with lesion Page4 Date Report.Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07/12/04 Lab: NCTR TDMS No. 20313 - 02 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-DAY ACRYLAMIDE CAS Number: 79-06-1 Pathologist: OLSON. G. Route: DOSED WATER Species/Strain: MICE/B6C3F1/NCTA C57BU6N XC3H/HEN MTV-NCTR MICE 3.52 Acryl M MALE INTEGUMENTAAY SYSTEM None MUSCULOSKELETAL SYSTEM Skeletal Muscle Atrophy (8) 3 (38%) NERVOUS SYSTEM Peripheral Nerve Axon, Degeneration Spinal Cord, Lumbar Axon, Degeneration (8) 8 (100%) (8) 8 (100%) RESPIRATORY SYSTEM None SPECiAi.. SENSES SYSTEM None URINARY SYSTEM Urinary Bladder Lumen, Dilatation (8) 8 (100%) *** END OF MALE *** a - Number of animals examined microscopically at site and number of animals with lesion Page 5 Date Re;p ort Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07/12104 Lab: NCTR TOMS No. 20313 - 02 Test Type: 90-DAY P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR C"78~J6N XC3H/HEN MTV--NCTR MICE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12104 / 07/12104 Lab: NCTR Pathologist: OLSON, G. Control Water F 0.14 Acryl F 0.35 Acryl F 0.70 Acryl F 1.41 Acryl F 8 8 8 8 8 8 8 8 8 8 8 8 FEMALE Disposition Summary Animals Initially in Study Early Deaths Survivors Terminal Sacrifice Animals Examined Microscopically 8 1 ALIMENTARY SYSTEM Liver Mineralization Tension lipidosis (8) (0) (1) 1 (100%) (0) (0) (8) (0) (0) (0) (0) (8) 8 (100%) (0) (0) (0) (0) (8) (0) (1) (0) (8) (1) (0) CARDIOVASCULAR SYSTEM Heart Mineralization 1 (13%) ENDOCRINE SYSTEM Adrenal Cortex Subcapsular, Hyperplasia GENERAL BODY SYSTEM None GENITAL SYSTEM Ovary Anestrus Cyst 1 (100%) HEMATOPOIETIC SYSTEM Spleen (8) (0) a - Number ot animals examined microscopically at site and number of animals with lesion Page6 (0) TOMS No. 20313 - 02 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE First Dose M/F: 07/ 12/04 / 07/12104 Lab: NCTR Pathologist: OLSON, G. Control Water F 0.14 Acryl F Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 0.35 Acryl F Pigmentation 0.70 Acryl F 1.41 Acryl F 1 (100%) INTEGUMENTARY SYSTEM None MUSCULOSKELETALSYSTEM Skeletal Muscle Atrophy (8) (0) (0) (8) (8) (8) (0) (O) (8) (8) (8) (0) (0) (O) (8) (8) (0) (0) (1) (O) NERVOUS SYSTEM Peripheral Nerve Axon, Degeneration Spinal Cord, Lumbar Axon, Degeneration RES P l RATORY SYSTEM None SPECIAL SENSES SYSTEM • Eye Lens, Developmental Malformation 1 (100°k ) -URINAR t ' SYSTEM Urinary B ladder Lumen, Dilatation (8) (0) a - Number of animals examined microscopically at site and number of animals with lesion Page 7 (0) (0) (0) TOMS No. 203 t 3 - 02 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-DAV ACRVLAMIDE Route: DOSED WATER CAS Number: 79--06-1 Species/Strain: MICE/86C3F1/NCTR C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE Pathologi st: OLSON, G. 3.52 Acryl F Disposition Summary Animals Initially in Study Early Deaths Survh1vrs Terminal Sacrifice Animals Examined Microscopically 8 8 8 ALIMENTARY SYSTEM Liver Mineralization Tension Lipidosis (8) 1 (1 3%} CARDIOVASCULAR SYSTEM Heart Mineralization (8) ENDOCRINE SYSTEM Adrenal Cortex Subcapsular, Hyperplasia (8) 8 (100%) GENERAL BODY SYSTEM None GENITAL SYSTEM Ovary Anestrus (8) 6 (75%) Cyst HEMATOPOIETIC SYSTEM Spleen (8) a - Number of animals examined microscopically at site and number of animals with lesion Page8 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:1 5:54 First Dose M/F: 07112/04 I 07/12/04 Lab: NCTR TOMS No. 20313 - 02 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-0AY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE Pathologist; OLSON, G. 3.52 Acryl F Pigmentation INTEGUMENTARY SYSTEM None MUSCULOSKELETALSYSTEM Skeletal Muscle Atrophy (8) 5(63%) NERVOUS SYSTEM Peripheral Nerve Axon, Degeneration Spinal Cord, Lumbar Axon, Degeneration (8) 8 (100%) (8) 7 (88%) RESPIRATORY SYSTEM None SPECIAL SENSES SYSTEM Eye Lens, Developmental Malformation (8) URINARY SYSTEM Urinary Bladder Lumen, Dilatation (8) 4 (50%) *** END OF REPORT *** a - Number of animals examined microscopically at site and number of animals with lesion Page9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose MJF: 07/12104 / 07/12/04 Lab: NCTR P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) GLYCIDAMIDE CAS Number: 5694-00-8 TOMS No. 20314 - 02 Test Type: 90-DAY Route: DOSED WATER Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. C Number: C20314 Lock Date: Not Entered. Cage Range: ALL Date Range: ALL Reasons For Removal: ALL Removal Date Range: ALL Treatment Groups: Include ALL Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose MJF: 07/26/04 / 07/26/04 lab: NCTR TDMS No. 2031 ~ - 02 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-DAY Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 GLYCIDAMIDE Route: DOSED WATER CAS Number: 5694-Q0-.8 First Dose M/F: 07126/04 / 07126104 Species/Strain: MICE/86C3F1 /NCTR Pathologist: OLSON. G. Lab: NCTR C57BLJ6N XC3H/HEN MTV-NCTR MICE MALE Control Water M 0.14 Glycid M 0.35 Glycid M 0.70 Glycid M 1.41 Glycid M 8 8 8 8 8 8 8 2 8 2 8 8 8 8 (8) (0) (0) (0) (0) (0) (0) (0) (0) (8) (1) 1 (100%) (1) (0) (0) (8) (0) (0) (8) Disposition Summary Animals Initially in Study Early Deaths Survivors Terminal Sacrifice Animals Examined Microscopically ALIMENTARY SYSTEM Liver Tension Lipidosis 1 (13%) CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM Adrenal Cortex Accessory Adrenal Cortical Nodule (7) 1 (14%) GENERAL BODY SYSTEM None GENITAL SYSTEM Preputial Gland Cyst Duct, Dilatation Testes Germinal Epithelium, Degeneration 1 (100%) HEMATOPOIETIC SYSTEM None a - Number of animals examined microscopic1lly at site and number of animals with lesion Page2 (0) TOMS No. 20314 - 02 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) GLYCIDAMIDE Test Type: 90-DAY Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose MIF: 07/26/04 / 07126104 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR C57BU6N XC3HIHEN MTV·NCTR MICE Control Water M 0.14 Glycid M 0.35 Glycid M 0.70 Glycid M 1.41 Glycid M (8) (0) (1) 1 (100%) 1 (1 00%) (0) (0) (8) (0) (0) (0) (0) (8) {O) (0) (0) (0) (8) (0) (0) (0) (0) MALE INTEGUMENTARY SYSTEM Skin Inflammation, Chronic Active Fat, Necrosis MUSCULOSKELETAL SYSTEM None NERVOUS SYSTEM Peripheral Nerve Axon, Degeneration RESPIRATORY SYSTEM Lung Inflammation, Chronic Active SPECIAL SENSES SYSTEM None URINARY SYSTEM Urinary Bladder Lumen, Dilatation a - Number of animals examined microscopically at site and number of animals with lesion Page3 TOMS No. 20314 - 02 Test Ty~'!: P03: INCIDENCE RATES OF NO~EPLASTIC 90-DAY LESIONS BY ANATOMIC SITE(a) GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-()()..8 First Dose M/F: 07/26/04 / 07/26/04 Species/Strain: MICEJ86C3F1/NCTR Pathologist: OLSON, G. Lab : NCTR C57BL16N XC3H/HEN MTV-NCTR MICE MALE 3.52 Glycid M Disposition Summary Animals Initially in Study Earl y Deaths Survivors Terminal Sacrifice Animals Examined Microscopically 8 8 8 ALIMENTARY SYSTEM Liver Tension Lipidosis (8) CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM Adrenal Cortex Accessory Adrenal Cortical Nodule (8) GENERAL BODY SYSTEM None GENITAL SYSTEM Preputial Gland Cyst Duct, Dilatation Testes Germinal Epithelium, Degeneration (8) (8) 7 (88%) HEMATOPOIETIC SYSTEM None a - Number of animals examined microscopically at site and number of animals with lesion Page4 TDMS No. 20314 - 02 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-0AY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26104 / 07126104 Species/Strain: MICE/B6C3F1 /NCTR Pathologist: OLSON, G . Lab: NCTR C57BU6N XC3H/HEN MTV-NCTR MICE MALE 3.52 Glycid M INTEGUMENTARY SYSTEM Skin Inflammation, Chronic Active Fat. Necrosis (8) -MUSCULOSKELETALSYSTEM None NERVOUS SYSTEM Peripheral Nerve Axon, Degeneration (8) 1 (13%) RESPIRATORY SYSTEM Lung Inflammation, Chronic Active (8) 1 (1 3%) SPECIAL SENSES SYSTEM None URINARY SYSTEM Urinary Bladder Lumen, Dilatation (8) 1 (13%) * 0 END OF MALE *** a - Number of animals examined microscopically at site and number of animals with lesion Pages TDMS No. 20314 - 02 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-0AY GLYCIDAMIDE Date Report Reqsted: 06/2912006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-oo-B First Dose M/F: 07/26/04 / 07/26/04 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON. G. Lab: NCTR C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE Control Water F 0.14 Glycid F 0.35 Glycid F 0.70 Glycid F 1.41 Glycid F 8 8 8 8 8 8 8 8 1 8 8 2 1 (0) (0) (0) (8) 1 (13%) (8) (0) Disposition Summary Animals Initially in Study Early Deaths Natural Death Survivors Terminal Sacrifice Animals Examined Microscopically 7 ALIMENTARY SYSTEM Mesentery Fat, Necrosis Pancreas Cyst Salivary Glands Infiltration Cellular, Lymphoid (0) (0) (1) 1 (100%) (0) (0) (0) (0) (1) (7) 1 (14%) 6 (86%) (0) (0) (0) (1) (8) (1) 1 {100%) (0) (1) 1 (100%) (1) (0) CARDIOVASCULAR SYSTEM None ENDOCRINE SY STEM Adrenal Cortex Accessory Adrenal Cortical Nodule Subcapsular. Hyperplasia GENERAL BODY SYSTEM None GENITAL SYSTEM Ovary Cyst a - Number of animals examined microscopically at site and number of animals with lesion Page 6 TOMS No. 20314 - 02 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-DAY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route : DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07126104 107126104 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTA C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE Control Water F 0.1 4 Glycid F 0.35 Glycid F 0.70 Glycid F 1.41 Glycid F (8) (0) (0) (0) (0) (8) (0) {O) (0) (O) HEMATOPOIETIC SYSTEM Thymus INTEGUMENTARY SYSTEM None MUSCULOSKELETALSYSTEM None NERVOUS SYSTEM Peripheral Nerve Axon, Degeneration RESPIRATORY SYSTEM None SPECIAL SENSES SYSTEM None URINARY SYSTEM None a - Number of animals examined microscopically at site and number of animals with lesion Page 7 TOMS No. 20314 · 02 P03: INCIDENCE RATES Test Type: 90-DAY OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00·8 First Dose MIF: 07/26/04 / 07/26/04 Species/Strain: MICEJB6C3F1 /NCTR Pathologist: OLSON, G . lab: NCTR C57Bl/6N XC3H/HEN MTV-NCTR MICE FEMALE 3.52 Glycid F Disposition Summary Animals Initially in Study Early Deaths Natural Death 8 s ~ : n-!;:ors Terminal Sacrifice Animals Examined Microscopically 8 8 ALIMENTARY SYSTEM Mesentery Fat, Necrosis Pancreas Cyst Salivary Glands Infiltration Cellular, Lymphoid (0) (8) (8) 1 (13%) CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM ~ Adrenal Cortex Accessory Adrenal Cortical Nodule Subcapsular, Hyperplasia (7) 4 (57%) GENERAL BODY SYSTEM None GENITAL SYSTEM Ovary Cyst (8) a - Number of animals examined microscopically at site and number of animals with lesion Page8 TOMS No. 20314 - 02 Test Type: 90-DAY P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Route: DOSED WATER CAS Number: 5694-00-8 First Dose MIF: 07/26/04 / 07/26/04 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE GLYCIDAMIDE 3.52 Glycid F HEMATOPOIETIC SYSTEM Thymus (8) INTEGUMENTARY SYSTEM None MUSCULOSKELETALSYSTEM None NERVOUS SYSTEM Peripheral Nerve Axon, Degeneration (8) 1 (13%) RESPIRATORY SYSTEM None SPECIAL SENSES SYSTEM None URINARY SYSTEM None *** END OF REPORT*** a - Number of animals examined microscopically at site and number of animals with lesion Page9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 TOMS No. 20313- 04 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-0S-1 Species/Strain: MICEJ86C3F1 /NCTR Pathologist: OLSON, G. C Number: C20313B Lock Date: Not Entered. Cage Range: ALL Date Range: ALL Reasons For Removal: ALL Removal Date Range: ALL Treatment Groups: Include ALL Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:06:38 Firs~ Dose M/F: 08102104108/02104 Lab: NCTR TOMS No. 20313 - 04 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-DAY AC RYLAMIDE Route: DOSED FEED CAS Number: 79-06· 1 C57BU6N XC3H/HEN MTV-NCTR MICE MALE Time Report Reqsted : 12:06:38 First Dose M/F: 08/02/04 / 08/02/04 Pathologist: OLSON, G. Species/Strain: MICE/86C3F11NCTR Date Report Reqsted: 07/31/2006 lab: NCTR Control Feed M 18.5 PPM Acryl M 37 PPM Acryl M 74 PPM Acryl M 185 PPM Acryl M 8 8 8 8 8 8 8 7 1 8 8 2 7 8 (8) (0) (0) (0) (1) (8) (0) (0) (0) (8) (8) 3 (38%) (0) (0) (1) (1) Disposition Summary Animals Initially in Study Early Deaths Accidently Killed Natural Death Survivors Terminal Sacrifice Animals Examined Microscopically ALIMENTARY SYSTEM None CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM None GENERAL BODY SYSTEM None GENITAL SYSTEM Epididymis Hypospermia Testes Germinal Epithelium, Degeneration HEMATOPOIETIC SYSTEM Spleen Hyperplasia, Lymphoid a - Number of animals examined microscopically at site and number of animals with lesion Page2 TOMS No. 20313 - 04 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-DAY ACRYLAMIDE ·cAs Number: 79-06-1 Route: DOSED FEED Species/Strain: MICEJ86C3F1/NCTR C57BU6N XC3H/HEN MTV·NCTR MICE MALE First Dose MJF: 08/02/04 / 08/02104 Lab: NCTR Pathologist OLSON, G. Control Feed M 18.5 PPM Acryl M Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:06:38 37 PPM Acryl M 74 PPM AcryL M 185 PPM Acryl M INTEGUMENTARY SYSTEM None MUSCULOSKELETAL SYSTEM Skeletal Muscle Atrophy (8) (0) (0) (0) (8) (8) (0) (0) (0) (8) (8) (0) (0) (0) (8) (8) (0) (0) (O) (1) -NERVOUS SYSTEM Peripheral Nerve Axon, Degeneration Spinal Cord, Lumbar Axon, Degeneration RESPIRA10RY SYSTEM None SPECIAL SENSES SYSTEM None - URINARY SYSTEM Urinary Bladder Lumen, Dilatation a - Number of animals examined microscopically at site and number of animals with lesion Page 3 TOMS No. 2031 3 - 04 Test Type: 90-DAY Route: ;":C$i:D FEED Species/Strain: MICE/B6C3F1/NCTR C57BL/6N XC3H/HEN MTV-NCTR MICE MALE P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) ACRYLAMIDE CAS Number: 79·06-1 Pathologist: OLSON, G. 370 PPM Aery! M Disposition Summary Ani mals Initially in Study Early Deaths Accidently Killed Natural Death Survivors Terminal Sacrifice Animals EJCamined Microscopically 8 7 8 ALIMENTARY SYSTEM None CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM None GENERAL BODY SYSTEM None GENITAL SYSTEM Epididymis Hyposperm1a Testes Germinal Epithelium, Degeneration (7) 3 (43%) (7) 7 (100%) HEMATOPOIETIC SYSTEM Spleen Hyperplasia, Lymphoid (7) a - Number of animals examined microscopically at site and number of animals with lesion Page4 Date Report Reqsted: 0713112006 Time Report Reqsted: 12:06:38 First Dose M/F: 08/02/04 / 08/02104 Lab: NCTR TOMS No. 20313 • 04 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Patho logist: OLSON, G. Species/Strain: MICE/86C3F1 /NCTR C57BU6N XC3HIHEN MTV-NCTR MICE MALE 370 PPM Acryl M INTEGUMENTARY SYSTEM None MUSCULOSKELETALSYSTEM Skeletal Muscle Atrophy (7) 7 (100%) NERVOUS SYSTEM Peripheral Nerve Axon, Degeneration Spinal Cord, Lumbar Axon, Degeneration (7) 5 (71 %) (7) 2 (29%) RESPIRATORY SYSTEM None SPECIAL SENSES SYSTEM Norn~ URINARY SYSTEM Urinary Bladder Lumen, Dilatation (7) 7 (100%) *** END OF MALE *** a - Number of animals examined microscopically at site and number of animals with lesion Pages Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:06:38 First Dose M/F: 08/02/04 / 08/02104 Lab: NCTR TOMS No. 20313 - 04 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-0AY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 C57BU6N XCJH/HEN MTV-NCTR MICE FEMALE nme Report Reqsted: 12:06:38 First Dose M/F: 08102104 108102104 Pathologist: OLSON, G. Species/Strain: MICE/B6C3F1/NCTR Date Report Reqsted: 07/31/2006 Lab: NCTR Control Feed F 18.5 PPM Acryl F 37 PPM Acryl F 74 PPM Acryl F 185 PPM Acryl F 8 8 8 8 8 8 8 8 8 8 Dispositlon Summary Animals Initially in Study Early Deaths Survivors Terminal Sacrifice Animals Examined Microscopically 8 8 ALIMENTARY SY STEM None -CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM Adrenal Cortex Accessory Adrenal Cortical Nodule Subcapsular, Hyperplasia (0) (0) (0) (O} (8) (0) (0) (0) (8) (8) (0) (0) (0) (0) (8) (0) (0) (0) (0) (8) 5 (63%) GENERAL BODY SYSTEM None GENITAL SYSTEM Ovary Anestrus Cyst HEMATOPOIETIC SYSTEM Lymph Node, Mandibular Hyperplasia, Lymphoid Lymph Node, Mesenteric a - Number of animals examined microscopically at site and number of animals with lesion Page 6 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 02 Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR ·Pathologist: OLSON, G. I1 lg ............................... DAVON TEST C578U6N XC3H/HEN MTV-NCTR MICE FEMALE ~ o 1 0 0 1 0 0 1 0 0 Hf.f 1 1 0 1 ·1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose MJF: 07/12/04 / 07112/04 Lab: NCTR 0 .......... ............. 8 3 4 8 1 8 2 2 3 14 + + + + + + + + 8 Bone, Femu r + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 ANIMAL ID Control Water F Skin 7 1 7 17 7 • TOTALS MUSCULOSKELETAL SYSTEM NERVOUS SYSTEM • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present .. Insufficient tissue Page24 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 02 Test Type: 90-DAY ACAYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST 01 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04/ 07/12/04 Lab: NCTR 110 110 10 11 0 0 11 0 11 0 11 0 0 0 0 0 0 0 0 0 0 7 1 0 0 0 0 0 0 0 0 7 0 0 0 2 3 4 0 0 0 8 1 8 2 0 0 0 8 3 8 4 Lung + + + + + + + + 8 Nose + + + + + + + + 8 Trachea + + + + + + + + 8 Eye + + + + + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + ~ + + + + + + 8 Urinary Bladder + + + + + + + + 8 + + + + + + + + 8 0 ......................... ..... ...... . .... .................. .... .................................................................................................... C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID Control Water F 0 7 17 0 0 0 * TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM URINARY SYSTEM SYSTEMIC LESIONS Multiple Organ M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Nol examined microscopically • .. Total animals with tissue examined microscopieally; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 25 TDMS No. 20313- 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIOE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE186C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST 1 0 0 ......................... ..... C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 0.35 Acryl F 0 0 1 5 1 1 0 0 1 0 0 1 0 0 1 0 0 1 0 0 1 1 0 0 0 0 0 0 0 1 1 6 3 6 4 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose MIF: 07/12/04 / 07/12/04 Lab: NCTR 0 .... ....... . .. .................................. 0 0 1 5 2 0 0 1 5 3 0 0 1 5 4 0 0 1 6 1 0 0 1 6 2 *TOTALS ALIMENTARY SYSTEM Liver + 1 + 1 CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAl SYSTEM Ovary HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM NONE M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscop:cally · x .. Lesion present I .. Insufficient tissue Page 26 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE186C3F1/NCTR Pathologist: OLSON, G. DAy ON TEST C57BU6N XC3H/HEN ~;· 1 1 01 1 0 1 I1 0 1 0 0 0 10 0 · ·~- MICE FEMALE ANIMAL ID 0.35 Acryl f I -~ l ·~ · ·~ 01 0 -~ · ·~ First Dose MIF: 07112/04 / 07/ 12/04 Lab: NCTR I0 1 10 1 1 01 0 0 0 · · ·~ ·~ ·· 0 0 0 0 0 0 0 0 1 5 1 5 2 1 5 1 5 1 1 1 1 3 4 1 2 3 4 1 Date Report Reqsted: 06129/2006 Time Report Reqsted: 08:15:54 ·· ·· 6 6 616 *TOTALS MUSCULOSKELETALSYSTEM NONE NERVOUS SYSTEM NONE RES?H~ATOY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE SYSTEMIC LESIONS Multiple Organ 1 + • .. Total animals with tissue examined microscopically; Total animals with tumor M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically + ·- Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 27 TOMS Nu. 2:1'.:l13 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL ACRYLAMIDE CAS Number: Pathologist: OLSON, G. Test Type: 90-DAY Route: DOSED WATER 79-06-1 Species/Strain: MICE/86C3F1/NCTA DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 0.70 Acryl F Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07112/04 / 07112/04 Lab: NCTR 10 11 0 1 11111 0 0 0 11 0 11 0 0 11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 2 0 0 0 0 01 1 02 2 0 2 0 3 4 1 1 1 1 9 9 9 9 1 2 3 4 * TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM NONE HEMATOPOIETIC SYSTEM Spleen 1 + INTEGUMENTARYSYSTEM NONE MUSCULOSKELETALSYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 28 TOMS No. 20313 -02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose MJF: 07/12/04 / 07/12/04 ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1 /NCTR ·Pathologist: OLSON, G. DAY ON TEST Lab: NCTR 1110 1 01 1111111· 0 0 0 0 ,·1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 0 3 1 1 1 1 2 2 0 2 0 4 9 , , 9 2 9 3 9 4 0 1 + + + + + + + + 8 + + + + + + + + 8 ························-····················· ............................................................................... C57BLJ6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 0.70 Acryl F Skeletal Muscle I 0 *TOTALS NERVOUS SYSTEM Peripheral Nerve RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM Eye 1 + URINARY SYSTEM NONE SYSTEMIC LESIONS Multiple Organ + + + + + + + 8 + • .. Total animals with tissue examined microscopically; Total animals with tumor M .. Missing tissue A .. Auto!ysis precludes evaluation BLANK .. Not examined microscopically + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 29 TOMS No. 20313- 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: M ICE/B6C3F1/NCTR Pathologist: OLSON, G. 11 DAYONTE!::iT C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 1.41 Acryl F l 1 1 1 l 1 1 Date Report Reqsted: 06/29/2006 Time Report R.e qsted: 08:15:54 First Dose M/F: 07/12104 / 07/12104 Lab: NCTR 1 l 1 oo o o o o o o 0 0 0 0 0 0 0 0 .................,................................................................................................................. o o o!o o o o o 0 0 0 0 1111 1 1 1 1 1 2 3 4 I 0 1 2 1 0 0 0 111 2 2 2 2 3 4 + + * TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM # Ovary + + + + + + 8 HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM 1 + Skin M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 30 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 02 Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07/12/04 Lab: NCTR 11111 1 1 1 111 0 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0 .......··r...................................................................................................... ............................................ DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE o0 o0 o0 o0 o0 o0 o0 o0 1 1 1 1 1 1 1 2 1 1 3 1 4 2 1 1 2 2 I 32 1 2 4 + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 ANIMALID 1.41 Acryl F 1 *TOTALS MUSCULOSKELETALSYSTEM Skeletal Muscle NERVOUS SYSTEM RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE SYSTEMIC LESIONS M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not e.xamined microscopically • .. Total animals with tissue examined microscopically: Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 31 TOMS No. 20313- 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Date Report Reqsted: 06/29/2006 ACRYLAMIDE CAS Number: 79-06-1 Time Report Reqsted: 08:1 5:54 R rst Dose M/F: 07/12/04 / 07/12/04 Test Type: 90-DAY Route: DOSED WATER Pathologist: OLSON, G. Species/Strain: MICE/B6C3F1/NCTR DAy ON TEST I 1111111 0 0 0 0 I Lab: NCTR 10 11111 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 1 1 1 11 11 0 0 1 2 1 2 3 4 1 2 Is + + + + + + + 0 0 0 0 0 0 1 2 0 0 1 2 0 0 1 2 ...................................··········1··········1···· .................................................................................................. C57BLJ6N XC3H/HEN MTV-NCTR MICE FEMALE 1.41 Acryl F ANIMAL ID Multiple Organ , I * TOTALS 4 + 8 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 32 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACAYLAMIDE CAS Number: 79-06-1 Route: DOSED WATER Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. 0 0 0 0 0 3 1 0 0 0 3 2 Esophagus + + Gallbladder ++ Intestine Large, Cecum + + Intestine Large, Colon + Intestine Large, Rectum Intestine Small, Duodenum 0 10 0 0 AN IMAL ID 3.52 Acryl F Lab: NCTR 0 0 0 0 0 0 0 0 0 0 0 4 3 0 0 0 3 4 1 0 0 0 4 2 0 0 0 4 3 0 0 0 4 4 + + + + + + 8 ++ M + ++ 7 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 .................... C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE First Dose M/F: 07112/04 / 07/1 2/04 Tl' 'l'l' l'I' 0 0 DAY ON TEST Dat e Report Reqsted: 06/29/2006 Time Report Reqst ed: 08:15:54 0 0 0 3 .............................. ....................................................................................... * TOTALS ALIMENTARY SYSTEM Intestine Small, Ileum ....................................................................................................................................................................................................... Intestine Small, Jejunum ··································································································································································································· Liver Pancreas + + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 ................................................................................................................................................. .................................................................................................................................................................................................... Salivary Glands ··········································································································································-························································ Stoma~h . Forestomach + + + + + + + 8 + M .. Missing tissue A .. Autotysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 33 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: Species/Strain: MICEJ86C3F1/NCTR .Pathologist: OLSON, G. DAY ON TEST C57BL16N XC3H/HEN 79-06-1 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose MIF: Lab: NCTR 07/12/04 /07/12/04 10 11 0 11 0 11 0 11 0 11 0 11 0 1·1 0 .o 0 0 0 0 0 0 0 · · ~ · · - ~ · ·~ · · 1 ..~· ·~ · 1 · ~- r ~· · ~· ~;· 0 0 0 3 1 3 2 3 3 Stomach, Glandular + + Tongue + + 0 0 0 10 0 o o o o o o o o MICE FEMALE ANIMAL ID I 3 4 14 I 4 3 4 4 + + + 8 + + + 8 Blood Vessel +M++++++ 7 Heart + + + + + + + + 8 Adrenal Cortex + + + + + + + + 8 Adrenal Medulla + + + + + + + + 8 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland ++M +++++ 7 Pituitary Gland M + + + + 7 Thyroid Gland + +M++ +++ 7 3.52 Acryl F 4 1 2 + + + + + + *TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM + + + GENERAL BODY SYSTEM • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals wi1h tumor .. Tissue examined microscopically •. Lesion present .. Insufficient tissue Page 34 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Date Report Reqsted: 06/29/2006 Test Type: 90-DAY Route: DOSED WATER ACRYLAMIDE Time Report Reqsted: 08:15:54 First Dose MIF: 07112104 107/12104 CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. 10 110 , 0, 110 0 0 0 0 01 0 0 0 0 0 0 0 0 10 0 0 0 0 0 0 0 3 1 3 2 3 3 3 I 4 4 1 Clitoral Gland + + + + Ovary + + + Uterus + + Bone Marrow + Lymph Node, Mandibular DAY ON TEST Lab: NCTR I 01 10 110 0 0 0 0 0 0 4 0 0 0 2 4 3 0 0 0 4 4 + + + + 8 + + + + + 8 + + + + + + 8 + + + + + + + 8 M + + + + + + + 7 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + + + + 8 + + + + + + + + 8 C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 3.52 Acryf F *TOTALS NONE GENITAL SYSTEM HEMATOPOIETIC SYSTEM . INTEGUMENTARY SYSTEM Mammary Gland M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. lnsutticient tissue Page 35 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Date Report Reqsted: 06/29/2006 ACRYLAMIDE Route: DOSED WATER Time Report Reqsted: 08:15:54 CAS Number: 79-06-1 Species/Strain: MICE186C3F1/NCTR First Dose M/F: 07/12/04 / 07/12/04 Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN ~; Lab: NCTR 0 1 10 1 10 1 1 01 1 01 1 0 1 1 01 \ 0 1 0 0 0 0 0 0 0 0 ·- · ·~ · ·~ T ·~ · ·~ - 1 ·~ 0 0 0 0 0 0 3 1 0 3 2 0 3 3 0 3 4 0 4 1 + + + + Bone, Femur + + + Skeletal Muscle + + Brain, Brain Stem + Brain, Cerebellum - -~· I · ·~ · · ··· ·· · · ·· · · · ·· ······ ·· · · · · · 0 0 0 0 4 2 0 4 3 0 4 4 + + + + 8 + + + + + 8 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 MICE FEMALE ANIMAL ID 3.52 Acryl F Skin j *TOTALS MUSCULOSKELETALSYSTEM NERVOUS SYSTEM • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor · .. Tissue examined microscopically .. Lesion present .. lnsllfficient tissue Page 36 TOMS No. 20313 • 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Date Report Reqst ed: 06/29/2006 ACAYLAMIDE Time Report Reqsted: 08:15:54 First Dose MJF: 07/12/04 I 07112104 Test Typ,.: 9C\-DAY Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. I DAy ON TEST C57BLJ6N XC3H/HEN ;~· · I I 111 I 111 1l 1 0 0 0 0 0 0 ·~ T ~ T ~· r ~· 0 0 0 0 4 2 4 3 0 o o o o o o olo ~ ·r 0 ~· ·~ 0 0 ·~ ·· · · · ·· · · ·· · ·· ·· · · ·· · 0 o o o o o o o o MICE FEMALE 3.52 Acryl F ··r 0 Lab: NCTR ANIMAL ID 3 314 I 313 1 2 3 4 1 4 14 • TOTALS RESPIRATORY SYSTEM Lung + + + + + + + + 8 Nose + + + + + + + + 8 Trachea + + + + + + + + 8 Eye + + + + + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 + + + + + + + + 8 SPECIAL SENSES SYSTEM URINARY SYSTEM SYSTEMIC LESIONS Multiple Organ M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor · + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 37 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 • 02 Test Type: 90-DAY Date Report Reqsted: 06/29/2006 ACRYLAMIDE Route: DOSED WATER Time Report Reqsted: 08:15:54 CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR 1 DAY ON TEST C57BU6N XC3H/HEN ~; ~ ~;· Lab: NCTR 0 0 · 1 0 0 · ~· 01 11 0 0 0 ·-~ 1 0 0 · · ·~ 0 0 0 3 1 3 2 3 1 0 1 1 0 0 0 0 0 1 · · ~· 0 0 · ·~ 0 · ·~ 0 ·· ·· · · ·· · · · · · · ·· · · ·· ·· · · ···· ·· ·· · · ··· · ·· 0 o o o o o o o o MICE FEMALE 3.52 Acryl F First Dose M/F: 07/12/04 / 07/12/04 Pathologist: OLSON, G. ANIMAL ID 313 4 4 414 1 2 3 4 4 * TOTALS *** END OF REPORT *** M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. lesion present I .. Insufficient tissue Page 38 TOMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL GLYCIDAMIDE Test Type: 90-DAY Date Report Reqsted: 06129/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26104 / 07126104 Species/Strain: MICE/86C3F1/NCTR ·Pathologist: OLSON, G. lab: NCTR C Number: C20314 Lock Date; Not Entered. Cage Range: ALL Date Range: ALL Reasons For Removal: ALL Removal Date Range: ALL Treatment Groups: Include ALL P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20314 - 02 Test Type: 90-DAY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07/26/04 Species/Strain: MICE/86C3F1/NCTR Path ologist: OLSON, G. Lab: NCTR DAY ON TEST C57BU6N XC3H/HEN ~ ~ ~; ·· MICE MALE I~ 0 9 9 00 0 9 9 010 9 9 9 9 0 9 9 4 2 4 4 2 1 2 4 2 3 4 2 4 9 9 9 9 4 4 3 .iTifg. ·g· ·i···g...i . . . . . . . . ... · ·i- 4 1 1 4 1 2 Esophagus + + + + + + + + 8 Gallbladder + + + + + + + + 8 Intestine Large, Cecum + + + + + + + + 8 Intestine Large, Colon + + + + + + + + 8 Intestine Large, Rectum + +++M+++ 7 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 Liver + + + + + + + + 8 Pancreas + + + + + + + + 8 Salivary Glands + + + + + + + + 8 Stomach, Forestomach + + + + + + + + 8 Control Water M ANIMAL ID 11 11 * TOTALS ALIMENTARY SYSTEM • + x I M .. Missing tissue A .. Autolys.is precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopieally; Total animals with tumor .. Tissue examined microscopically .. Lesion present .. Insufficient tissue Page 2 TOMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose MJF: 07/26/04 / 07126104 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. lab: NCTR DAy ON TEST I 09 9 II I I I I I 0 9 0 9 0 9 0 9 0 9 0 9 9 9 9 9 9 9 0 9 9 ........................1····· .............................................................................................................................. C578U6N XC3H/HEN MTV-NCTR MICE MALE Conirol Water M ANIMAL ID 0 0 4 11 1 o0 o0 0o o0 o0 o0 o0 4 1 2 4 1 3 I 4 1 4 4 2 1 4 4 2 2 3 4 2 4 2 · * TOTALS Stomach, Glandular + + + + + + + + 8 Tongue + + + + + + + + 8 CARDIOVASCULAR SYSTEM •••••••••••••••• • •••• • • • •••••·• -•••·•··•••••••u••••••••••••••••••••••••••'•••••••• • • •• •••••••••••••••••••••••• ••••• ••••••I•••••••••••• •••••••••••••••••••••• •••••••••• •••••••••• ••••••••••••••••••..,,••••••• Blood Vessel * + + + + + + + a· Heart + + + + + + + + 8 Adrenal Cortex M+++++++ 7 Adrenal Medulla M+++++++ 7 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland + + + + + + + + 8 Pituitary Gland +++++M++ 7 Thyroid Gland + 8 ENDOCRINE SYSTEM + + + + + + + GENERAL BODY SYSTEM • .. Total animals with tissue examined microscopically; Total animals with tumor M .. Missing tissue + .. Tissue examined microscopically A .. Autolysis precludes evaluation x .. Lesion present BLANK .. Not examined microscopically I .. Insufficient tissue Page3 TUMS No. 20314 · 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIOE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07126104107126104 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. lab:NCTR 0 0 9 9 9 9 0 0 4 1 0 0 9 9 9 9 0 9 9 0 0 4 0 0 4 0 0 4 1 1 2 1 3 Epididymis + + Preputial Gland + Prostate DAY ON TEST olo 0 9 19 9 9 9 9 0 0 4 0 0 4 0 0 4 0 0 4 1 4 2 1 2 2 2 3 2 4 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 Seminal Vesicle + + + + + + + + 8 Testes + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular + + + + + + + + 8 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + + + 8 C57BlJ6N XC3HJHEN MTV-NCTR MICE MALE ANIMAL ID Control Water M ............ ·················································································································· * TOTALS NONE GENITAL SYSTEM HEMATOPOIETIC SYSTEM + M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 4 TOMS No. 20314 - 02 Test Type: 90-DAY P04: NEOPLASMS BY INDIVIDUAL ANIMAL GLYCIDAMIDE CAS Number: 5694-00-8 Pathologist: OLSON, G. Route: DOSED WATER Species/Strain: MICEJ86C3F1/NCTA DAy ON TEST C57BU6N XC3H/HEN,;: ~: ~ ~ - MICE MALE Control Water M I 09 9 0 9 09 9 9 9 9 9 9 9 ..... g···gTi··-g ·g-···g· i ]i··············· 4 ANIMAL ID 010101010 9 9 9 9 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose MIF: 07/26/04 / 07126104 Lab: NCTR 1 1 4 1 2 4 1 3 4 1 4 4 4 4 4 1 2 3 4 2 2 212 * TOTALS INTEGUMENTARY SYSTEM Mammary Gland M M M M M M M M 0 Skin + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 MUSCULOSKELETALSYSTEM NERVOUS SYSTEM • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present .. Insufficient tissue Page 5 TOMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED WATER CAS Number: 5694-00-8 GLYCIDAMIDE · Pathologist: OLSON, G. Species/Strain: MICE/86C3F1 /NCTR DAY ON TEST ANIMAL ID 0 0 I 9 9 9 0 0 0 0 0 0 4 414 4 1 1 1 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07/26/04 Lab: NCTA I o9 o9 9o o9 9 C57BU6N XC3H/HEN MTV-NCTR MICE MALE I I I Date Report Reqsted: 06/29/2006 0 9 9 I 0 9 9 0 10 0 0 414 2 2 0 9 1 ·0 9 9 0 0 4 0 0 2 42 9 1 2 3 1 4 + + + + + + + + 8 Lung + + + + + + + + 8 Nose + + + + + + + + 8 Trachea + + + + + + + + 8 Eye + + + + + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 Control Water M Spinal Cord, Thoracic 1 2 3 4 *TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM URINARY SYSTEM SYSTEMIC LESIONS • + x I M .. Missing tissue .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present .. Insufficient tissue A .• Autolysis precludes evaluation BLANK .. Not examined microscopically Page6 TOMS No. 20314- 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 Rrst Dose MJF: 07/26/04 / 07/26/04 Species/Strain : MICE/86C3F1/NCTA Pathologist: OLSON, G. Lab: NCTR DAY ON TEST 1 09 1 09 09 09 09 09 1 09 1 09 9 9 9 9 9 9 9 9 4 . 4 4 4 4 4 4 4 1 1 3 4 1 2 3 4 ..............................1···· .......... .... ·····1······································································································· C57BIJ6N XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 o0 o0 o0 MICE MALE Control Water M ANIMAL ID Multiple Organ 1 1 11 2 2 21212 *TOTALS 8 ++++++++ M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopieally; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page? TOMS No. 20314 - 02 Test Type: 90-DAY Route: DUS~ WATER P04: NEOPLASMS BY INDIVIDUAL ANIMAL Gl.,YCIDAMIDE Species/St rain: MICE/B6C3F1/NCTR 0 DAY ON TEST 9 9 olo 0 0 9 9 9 9 9 9 9 9 3 3 3 3 8 3 8 1 2 0 9 9 Date Report Reqst ed: 06/29/2006 Time Report Reqsted: 08:34:07 CAS Number: 5694-00-8 First Dose M/F: 07126104107/26104 Pathologist.: OLSON, G. Lab: NCTR 0 9 9 0 9 9 ..............................f.........................,...................................................................................................... C57BLJ6N XC3H/HEN MTV-NCTR o0 o0 o0 Il o0 .00 o0 o0 o0 . MICE MALE 0.14 Glycid M 3 ANIMAL ID 71 72 717 3 4 I 3 8 3 8 3 4 *TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM Preputial Gland 1 + HEMATOPOIETIC SYSTEM Spleen 1 + INTEGUMENTARY SYSTEM NONE • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesic;m present .. Insufficient tissue Pages TOMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL GLYCIDAMIDE CAS Number: 5694-00-8 Test Ty pe: 90-DAY Route: DOSED WATER Species/Strain: MICE/86C3F1/NCTR Pat hologist: OLSON, G. DAy ON TEST I 90 1 90 1 09 1 90 1 90 1 90 Date Report Reqst ed: 06/29/2006 Time Report Reqsted : 08:34:07 First Dose M/F: 07/26104 / 07126104 Lab: NCTR 09 1 09 9 9 9 9 9 9 9 9 3 7 3 7 2 3 7 3 3 7 18 3 8 3 8 3 4 3 8 2 3 4 ·························1····· .... ·········· ............... ·································································································· o0 o0 o0 o0 o0 o0 o0 o0 • C57BU6N XC3H/HEN MTV-NCTR MICE MALE 0.14 Glycid M ANIMAL ID I 1 1 * TOTALS MUSCULOSKELETAL SYSTEM NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NC'NF: SYSTEMIC LESIONS Multiple Organ + 2 + M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page9 TDMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07126104 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. lab: NCTR - -- DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR · 0 0 0 0 0 10 10 9 9 9 9 9 9 9 9 9 9 9 0 0 0 0 0 9 9 · ·~ 1 ·~ 0 MICE MALE 0.35 Glycid M 0 4 ANIMAL ID 5 1 · ·~ · 0 I 9 0 9 ·~ 9 · ~· -~· · ·~ · · · 4 4 4 4 4 4 4 5 5 3 5 4 6 1 6 2 6 3 6 4 2 *TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM Preputial Gland + 1 HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM Skin 1 + • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present .. Insufficient tissue Page 10 TOMS No. 20314- 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Date Report Reqsted: 06/29/2006 GLYCIDAMIDE CAS Number: 5694-00-8 Time Report Reqsted: 08:34:07 Route: DOSED WATER Species/Strain : MICEIB6C3F1/NCTR ·Pathologist: OLSON, G. Lab: NCTR Test Type: 90-0AY DAy ON TEST First Dose M/F: 07/26/04 / 07/26/04 I :o I :o I ~o I :o I o~ I o~ I o~ ~ 1.0 .............................. ................................................................................................................................... C57BLJ6N XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 0o o0 o0 MICE MALE ANIMAL ID 0.35 Glycid M 4 5 1 4 5 2 4 5 3 4 5 4 4 6 1 I 4 6 2 4 6 3 4 6 4 + + *TOTALS MUSCULOSKELETALSYSTEM NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE SYSTEMIC LESIONS Multiple Organ 2 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 11 TOMS No. 20314 - 02 Test Type: 90-DAY P04: NEOPLASMS BY INDIVIDUAL ANIMAL GLYCIDAMIDE CAS Number: 5694-00-8 Route: DOSED WATER Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 1.41 Glycid M o9 , o9 o9 o9 0 0 0 0 2 5 0 0 2 6 4 + 2 5 1 0 0 2 2 5 3 + + Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07/26/04 Lab: NCTR I I 0 10 10 ! 0 9 9 9 19 9 9 9 9 0 0 2 5 Dat e Report Reqsted: 06/29/2006 9 19 9 9 0 0 2 0 0 2 0 0 2 1 6 2 6 3 6 4 + + + + * TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM 4 Testes + 8 HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM NONE MUSCULOSKELETALSVSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopieally; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 12 TOMS No. 20314 - 02 Test Type: 90-DAY Route: DOSED WATER P04: NEOPLASMS BY INDIVIDUAL ANIMAL G ~ YCIOAMIDE CAS Number: 5694-00-8 Pat hologist: OLSON, G. Species!Strain: MICE/86C3F1/NCTR DAY ON TEST ANIMAL ID 1.41 Glycid M 08:34:07 First Dose M/F: 07/26/04 / 07126104 Lab: NCTR 09 109 109 109 109 109 1010 9 9 9 C57BU6N XC3H/HEN MTV-NCTR MICE MALE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 9 9 9 9 0 0 0 0 0 9 9 9 0 2 5 0 2 5 0 2 5 0 2 5 0 2 6 0 0 2 6 0 0 2 6 0 0 2 6 1 2 3 4 1 2 3 4 + + + + + + + + * TOTALS NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE SYSTEMIC LESIONS Multiple Organ 8 M .. Missing tissue A ~ Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 13 TOMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07/26/04 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR DAY ON TEST I 0 9 9 0 9 9 01010 9 9 9 9 9 9 0 9 9 09 09 9 9 ····.············-··r···· ····················· ....1.................................................................................................. C57BU6N XC3H/HEN MTV-NCTR MICE MALE 3.52 Glycid M ANIMAL ID o0 o0 o0 o0 o0 o0 o0 0o 3 3 3 3 3 3 3 3 13 3 3 414 4 3 4 • TOTALS 1 2 3 4 1 2 3 4 Esophagus + + + + + + + + 8 G::l!tll:idder + + + + + + + + 8 Intestine Large, Cecum + + + + + + + + 8 Intestine Large, Colon + + + + + +· + + 8 Intestine Large, Rectum + + + + + + + + 8 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 Liver + + + + + + + + 8 Pancreas + + + + + + + + 8 Salivary Glands + + + + + + + + 8 Stomach, Forestomach + + + + + + + + 8 ALIMENTARY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 14 TOMS No. 2031 4 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose MJF: 07126104107126104 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR 0 DAY ON TEST C578Ll6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 3.52 Glycid M 9 9 0 09 1010 9 9 10 9 9 010 9 9 0 9 9 0 00 1 00 3 3 1 3 3 3 13 2 3 9 9 9 9 9 0 0 0 0 0 0 0 0 0 0 3 3 3 4 4 1 3 4 2 3 4 4 4 3 3 *TOTALS Stomach, Glandular + + + + + + + + 8 Tongue + + + + + + + + 8 Blood Vessel + + + + + + + + 8 Hear1 + + + + + + + + 8 Adrenal Cortex + + + + + + + + 8 Adrenal Medulla + + + + + + + + 8 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland +++M+M++ 6 Pituitary Gland + + + + + + + + 8 Thyroid Gland + + + + + + + + 8 CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM GENERAL BODY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 15 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20314 - 02 GLYCIDAMIDE Test Type: 90-0AY Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07/26/04 Species/Strain: MICE/86C3F1/NCTA ' Pathologist: OLSON, G. Lab: NCTR DAY ON TEST 09 109 109 010 9 9 9 9 9 C57BU6N XC3HIHEN MTV-NCTR MICE MALE 9 010 0 0 0 0 I o o -o 9 19 9 9 9 0 00 0 0 0 3 0 3 1 4 2 4 3 4 4 9 I 9 0 0 3 3 13 1 2 3 3 0 3 3 Epididymis + + + + + + + + 8 Preputial Gland i + + + + + + + 8 Prostate + + + + + + + + 8 Sc:n!m l Vesicle + + + + + + + + 8 Testes + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular + + + + + + + + 8 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + + + + 8 ANIMAL ID 3.52 Glycid M 3 3 4 3 4 3 *TOTALS NONE GENITAL SYSTEM HEMATOPOIETIC SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 16 P04:NEOPLASMSBY INDIVIDUAL ANIMAL TOMS No. 20314 - 02 Test Type: 90-DAY GLYCIDAMIDE Route: DOSED WATER Sp ce ies/ ~ ra .! n : CAS Number: 5694-00-8 MICE/B6C3F1/NCTR Pathologist: OLSON, G. DAVON TEST 0 9 0 9 Dat e Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07/26/04 Lab: NCTR 010101010 9 9 9 9 9 10 9 9 ' 9 . 9 ······························ ....,............... ·····r···· .................................................................................................. C57BLJ6N XC3H/HEN MTV-NCTR MICE MALE 9 9 9 9 9 o0 o0 o0 o0 o0 o0 o0 o0 3 3 3 3 3 3 3 3 3 1 3 3 4 4 1 4 2 3 3 2 4 3 4 4 Mammary Gland M M M M M M M M 0 Skin + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 ANIMAL ID 3.52 Glyc id M *TOTALS INTEGUMENTARY SYSTEM MUSCULOSKELETALSYSTEM NERVOUS SYSTEM . M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopicially; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 17 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Date Report Reqsted: 07/31/2006 Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Pathologist: OLSON, G. Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 TOMS No. 20313 - 04 Species/St rain: MICE/86C3F1 /NCTR DAY ON TEST I Lab: NCTR 1111111 0 0 0 1111 0 0 111 0 0 0 6 6 6 .6 6 1 1 6 1 2 6 1 3 Esophagus + + Gallbladder + Intestine Large, Cecum 6 6 6 6 6 1 4 6 6 2 12 1 2 6 2 3 6 2 4 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 Intestine Large, Colon + + + + + + + + 8 Intestine Large, Rectum + + + + + + + + 8 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 Liver + + + + + + + + 8 Pancreas + + + + + + + + 8 Salivary Glands + + + + + + + + 8 Stomach, Forestomach + + + + + + + + 8 ·····-················--······1····· ............................................................................................................................ C57BU6N XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 o0 o0 o0 MICE MALE Control Feed M ANIMAL ID I *TOTALS ALIMENTARY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. L esi~n present I .. Insufficient tissue Page2 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED FEED ACRYLAMIDE CAS Number: 79-06-1 Pathologist: OLSON, G. Species/Strain: MICE/86C3F1 /NCTR I 1 ' 1 I 1 1 I 1 1 1 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 I 08102104 Lab: NCTR 1 ololo o o o o o 6 6 6 6 6 6 6 6 ................................................... ......................................................................................................................... C578Uoi\J XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 o0 o0 o0 DAY ON TEST 6 s s s 6 s s s 1 1 1 2 1 3 1 4 2 1 2 2 2 I 2 3 4 Stomach, Glandular + + + + + + + + 8 Tongue + + + + + + + + 8 Blood Vessel + + + + + + + + 8 Heart + + + + + + + + 8 Adrenal Cortex + + + + + + + + 8 Adrenal Medulla + + + + + + +. + 8 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland ++MM+++M Pituitary Gland + + + + + + + + 8 Thyroid Gland + + + + + + + + 8 MICE MALE ANIMAL ID Control Feed M * TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM 5 GENERAL BODY SYSTEM • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present .. Insufficient tissue Page 3 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 04 Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTR Date Report Reqsted: 07131/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 I 08/02104 Pathologist: OLSON, G. Lab: NCTR 1 DAY ON TEST 111 1110 110 0 0 11111 0 0 0 0 6 C57BU6N XC3H/HEN ~ -· ANIMAL ID 6 16 r -~· 0 MICE MALE Control Feed M · ~· 6 6 6 1 · ·~ · · ·~ s s 0 0 0 6 6 1 1 2 3 4 11 1 11 I 6 6 · ~ · -~· ·· 0 0 0 0 2 1 2 2 2 3 2 4 ··· · ·· ·· ·· · · ·· ·· · · ··· ·· ·· ·· ..····· s s s s * TOTALS NONE GENITAL SYSTEM Epididymis + + + + + + + + 8 Preputial Gland + + + + + + + + 8 Prostate +++M+++M 6 Seminal Vesicle + + + + + + + + 8 Testes + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular + + + + + + + + 8 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + + + + 8 HEMATOPOIETIC SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 4 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TDMS No. 20313 - 04 Test Type: 90-DAY ACAYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID Control Feed M 1 0 6 lg 6 ,, 1 1 1 0 6 1 0 11 0 0 1 1 0 0 0 6 6 6 6 6 0 0 6 1 2 0 0 0 0 6 0 0 6 0 0 6 0 0 0 6 0 6 1 4 2 1 2 3 2 4 6 6 1 3 1 I 22 Date Report Reqsted: 07/31 /2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 Lab: NCTR * TOTALS INTEGUiv1ENTARY SYSTEM Mammary Gland M M M M M M M M 0 Skin + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 MUSCULOSKELETAL SYSTEM NERVOUS SYSTEM • .. Total animals with tissue examined microscopically; Total animals with tumor M .. Missing tissue A .. Aulolysis precludes evaluation BLANK .. Not examined microscopically + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Pages TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Date Report Reqsted: 07/31/2006 nme Report Reqsted: 12:29:56 ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1 /NCTR First Dose M/F: 08/02/04 / 08/02/04 Pathologist: OLSON, G. 1 DAY ON TEST 1 6 C57BU6N XC3H/HEN ~; · ·· ·· MICE MALE ANIMAL ID I1 I1 I o o o o · ~· 6 6 16 1 1 0 0 1 0 6 16 I 6 6 1 ·~- 1 0 · ~ . f · ~ · · ~· ·· · · 0 0 0 0 0 0 0 0 6 6 1 6 6 6 11 1 12 6 2 6 3 4 1 2 3 6 2 4 1 Lab: NCTR 2 · · ·· ·· ·· ···· · ·· ·· ·· · · ·· · · · · · · 1 I2 + + + + + + + + 8 Lung + + + + + + + + 8 Nose + + + + + + + + 8 Trachea + + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 Control Feed M Spinal Cord, Thoracic * TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM Eye • Harderian Gland URINARY SYSTEM SYSTEMIC LESIONS M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 6 TOMS No. 20313- 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACAYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1 /NCTR Pathologist: OLSON, G. DAY ON TEST ANIMAL ID Control Feed M Multiple Organ • + x I First Dose M/F: 08/02104 / 08/02104 Lab: NCTR 111 0 0 1 0 111111111 0 0 0 0 0 6 C57BU6N XC3H/HEN MTV-NCTR MICE MALE Date Report Reqsted: 07/ 31 /2006 Time Report Reqsted: 12:29:56 6 6 -6 6 6 ···grgr g·1 ··g···£ - ~ T 6 1 6 1 616 1 1 6 6 £ + + + + ..~ 616 26 2 2 2 1234123 + 6 + + * TOTALS 4 8 + M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present __ Insufficient tissue Page7 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90­DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79­06­1 Species/Strain: MICEIB6C3Fl/NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN MTV­NCTR MICE MALE ANIMAL ID 18.5 PPM Acryl M 1 0 Io 1 1 0 1 0 I0 I 0 6 9 6 6 6 16 0 0 6 5 0 0 6 5 0 0 6 5 0 0 0 6 5 0 6 6 1 2 3 4 1 1 1 First Dose M/F: 08/02/04 / 08/02104 Lab.: NCTR 1 \ 1 0 6 0 6 0 0 0 0 6 6 6 6 2 6 3 6 4 0 0 Date Report Reqsted: 07131/2006 Time Report Reqsted: 12:29:56 *TOTALS ALIMENT ARY SYSTEM Esophagus M 0 Gallbladder M 0 Intestine Large, Cecum M 0 Intestine Large, Colon M 0 Intestine Large, Rectum M 0 Intestine Small. Duodenum M 0 Intestine Small, Ileum M 0 Intestine Small, Jejunum M 0 Liver A 0 Pancreas M 0 Salivary Glands M 0 Stomach, Forestornach 0 M o o o o o o o O O o o o o o o o o o O O • O • O O o O O O • • O O O OO OOOOOOOOOOOOOOOOOOOl 0 0 0 0 0 0 0 0 0 0 • 0 0 0 0 0 0 0 0 0 0 0 0 0 o O o O O O O • O O O O O O O OA OOOO . . o o O o o o 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 o 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 o o o 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 o oO O O o 0 0 0 0 0 0 0 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. lnsuflicienl tissue Page8 000 000o o TOMS No. 20313 • 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Ro ute: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1 /NCTR Pathologist: OLSON, G. DAY ON TEST C57Bll6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 18.5 PPM Acryl M Stomach. Glandular I 11 0 01 I 01 1 0 1 0 6 6 1 0 6 0 0 0 0 6 0 0 0 0 6 6 6 6 3 4 1 0 01 6 9 6 ' 6 6 0 0 6 5 0 0 0 6 0 l 2 5 6 5 3 0 0 6 5 4 6 6 1 6 2 Date Report Reqsted: 07131/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08102/04 108102104 Lab: NCTA * TOTALS M 0 ····························································································································································································· M 0 Blood Vessel M 0 Heart M 0 Adrenal Cortex M 0 Adrenal Medulla M 0 Islets, Pancreatic M 0 Parathyroid Gland M 0 Pituitary Gland M 0 Thyroid Gland M 0 Tongue CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM GENERAL BODY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page9 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90·DAY Date Report Reqsted: 07/31/2006 ACAYLAMIDE Route: DOSED FEED Time Report Reqsted : 12:29:56 CAS Number: 79-06·1 Species/Strain: MICE/86C3F1/NCTR First Dose M/F: 08/02/04 / 08/02/04 Pathologist: OLSON, G. DAVON TEST 1 1 1 I 1 Lab: NCTR I I I I ~ ....... .. .. ... ... ... ... .................. ....................... 0 1 9 ~-. C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 18.5 PPM Acryl M ,I ~ 60 -~· 60 ~ 0 6 60 -~ 60 -~·. 0 6 0 6 0 6 0 6 5 1 5 2 5 15 16 3 4 1 1 0 6 I .-~ 0 6 1 0 6 -~ · 0 6 0 6 6 16 2 3 6 4 * TOTALS NONE GENITAL SYSTEM Epididymis M 0 Preputial Gland M 0 Prostale M 0 Seminal Vesicle M 0 Testes A 0 Bone Marrow M 0 Lymph Node, Mandibular M 0 Lymph Node, Mesenteric M 0 Spleen M 0 Thymus M 0 HEMATOPOIETIC SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 10 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACAYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICEJB6C3F1 /NCTR Pathologist: OLSON, G. I DAY ON TEST C57BU6N XC3HJHEN MTV-NCTR MICE MALE ANIMAL ID 18.5 PPM Acryl M 1 0 1 0 1 0 0 16 9 6 6 0 0 6 5 1 , 11 0 11 0 o 1o I o 0 0 6 16 0 5 15 2 3 5 4 6 6 6 6 6162 1 First Dose M/F: 08/02/04 / 08102/04 Lab: NCTR , I, 0 6 0 010 0 0 0 6 Date Report Reqsted: 07/31 /2006 Time Report Reqsted: 12:29:56 6 6 3 0 6 0 0 6 6 * TOTALS 4 INTEGUMENTARY SYSTEM Mammary Gland M 0 Skin M 0 Bone, Femur M 0 Skeletal Muscle A 0 Brain, Brain Stem M 0 Brain, Cerebellum M 0 Brain, Cerebrum M 0 Peripheral Nerve A 0 Spinal Cord, Cervical M 0 Spinal Cord, Lumbar M 0 MUSCULOSKELETALSYSTEM NERVOUS SYSTEM 4 • .. Total animals with tissue examined microscopically: Total animals with tumor M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 11 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 2031 3 - 04 Test Type: 90-DAY ACRYLAMIDE CAS Number: 79-06-1 Route: DOSED FEED Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST 1 0 6 Io 1 9 1 ······· ...... C57BU6N XC3H/HEN MTV-NCTB MICE MALE ANIMAL ID 18.5 PPM Acryl M 0 0 6 5 1 1 0 6 01 1 01 1 01 1 01 1 01 ·6 6 6 6 6 0 0 0 Date Report Reqsted: 07/31 /2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08102/04 / 08102104 Lab: NCTA .......... ............................ 0 0 6 5 2 0 0 6 6 0 6 6 5 3 5 4 1 0 0 6 6 2 0 0 6 6 3 0 0 6 6 4 *TOTALS ................... M 0 Lung A 0 Nose M 0 Trachea M 0 Eye M 0 Harderian Gland M 0 Kidney A 0 Urinary Bladder M 0 Spinal Cord, Tho racic RESPIRATOBY SYSTEM SPECIAL SENSES SYSTEM UBINARY SYSTEM SYSTEMIC LESIONS • .. Total animals with tissue examined microspaly~ + .. Tissue examined microscopically M .. Missing tissue A .. Autolysis p recludes evaluation BLANK .. Not examined microscopically Total animals with tumor x .. Lesion present I .. Insufficient tissue Page 12 TOMS No. 20313 · 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90·DAY ACAYLAMIDE Route: DOSED FEED CAS Number: 79·06-1 Species/Strain: MICEJB6C3F1/NCTR Pathologist: OLSON, G. '1 DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 18.5 PPM Aery! M I0 I 1 1 1 1 0 0 0 6 9 6 6 s 0 0 6 0 6 0 6 0 10 0 6 1 2 3 1 0 0 s s s 1s 4 I I, 0 1 1 0 0 6 sI 0 0 0 6 6 1 6 6 2 0 0 6 0 Dat e Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:S6 First Dose M/F: 08102/04 I 08102104 Lab: NCTR 6 0 0 6 6 6 3 4 * TOTALS ...................................................................................................................................................................................................... 0 Multiple Organ M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 13 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVlDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3Fl /NCTR Pathologist: OLSON, G. DAy ON TEST I6 1 0 Date Report Reqs ted: 07/3112006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02104 / 08/02104 Lab: NCTR 10 1 0 1 10 1 1 01 1 01 1 01 1 01 6 6 6 6 6 6 6 .......................................................·r..................................................-...................-............................... C57BL/6N XC3H/HEN MTV-NCTR MICE MALE 7 4 PPM Acryl M ANIMAL ID I I 0 0 0 0 0 0 7 7 7 7 8 1 2 3 4 1 0 0 0 0 I 0 0 0 0 0 0 8 8 3 4 s s s s s s s s 8 I2 I * TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM NONE HEMATOPOIETIC SYSTEM 1 + Spleen INTEGUMENTARYSYSTEM NONE MUSCULOSKELETALSYSTEM • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present .. Insufficient tissue Page 14 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 04 Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1 /NCTR 'P athologist: OLSON, G. 1 DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 7 4 PPM Acryl M I 1 o6 o6 01 6 0 0 0 0 5 7 1 5 7 2 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 lab: NCTR 110 11 0 0 11 0 0, 11 6 6 6 6 6 0 0 0 0 0 0 0 0 0 0 0 0 5 7 3 5 7 4 5 5 5 5 8 8 8 8 1 2 3 4 * TOTALS NONE NERVOUS SYSTEM Brain, Cerebrum + 1 + 2 RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE SYSTEMIC LESIONS Multiple Organ + M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopir.ally x .. Lesion present I .. Insufficient tissue Page 15 TOMS No. 20313 - 04 Tes t Type: 90-DAY P04: NEOPLASMS BY INDIVIDUAL ANIMAL Route: DOSED FEED CAS Number: 79-06-1 Date Report Reqsted: 07/31 /2006 Ti me Report Reqsted: 12:29:56 First Dose MJF: 08/02104 / 08102104 ACRYLAMIDE Species/Strain: MICE/86C3F1/NCTR Pat hologist: OLSON, G. Lab: NCTR I 1 111!1l 1, 1 1 0 0 0 0 0 0 1 0 DAY ON TEST C57BU6N XC3H/HEN ~ ; ~ ~ ~ ~ ; ~ · ·· 1 · · ~· 6 - ~· 6 ·~ 6 · ·~ 6 -· ~· 6 6 ·~- ·· · 0 0 0 0 0 0 0 13 1 3 2 3 3 3 4 4 1 4 2 4 3 0 s s s s s s s s MICE MALE 185 PPM Acryl M 2 1 ~ ANIMAL ID I ·· ·· ·· · ·· ·· ···· ·· . 4 4 * TOTALS ALIMENTARY SYSTEM Esophagus + 1 Gallbladder + 1 lnt£>sHr.e Large, Cecum + 1 Intestine Large, Colon + 1 Intestine Large, Rectum + 1 Intestine Small, Duodenum + 1 Intestine Small, Ileum + 1 Intestine Small, Jejunum + 1 Liver + Pancreas + Salivary Glands + 1 Stomach, Forestomach + 1 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 16 TOMS No. i!\3~ P04: NEOPLASMS BY INDIVIDUAL ANIMAL 3 • 04 Test Type: 90·DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICEJ86C3F1/NCTA Pathologist: OLSON, G. DAy ON TEST C57BLJ6N XC3H/HEN MICE MALE I1 0 2 Date Report Reqsted: 07/31/2006 n me Report Reqsted: 12:29:56 First Dose M/F: 08/02104 / 08/02104 Lab: NCTR 11111 1111111 0 0 0 I0 0 0 0 6 6 -6 3 2 3 3 , 6 6 6 6 4 2 4 3 4 4 .............................................. ............... ·································································································· MTV-NCTR o0 o0 o0 o0 o0 o0 o0 o0 5 s s s s s s s ANIMAL ID 3 14 1 I 185 PPM AeryI M 3 1 Stomach, Glandular + 1 Tongue + 1 Blood Vessel + 1 Heart + I4 *TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM Adrenal Cortex + Adrenal Medulla + Islets, Pancreatic + Parathyroid Gland M 0 Pituitary Gland + 1 Thyroid Gland + 1 1 GENERAL BODY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 17 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED FEED CAS Number: 79-06-1 ACRYLAMIDE Species/Strain: MICE/86C3F1 /NCTR Pathologist: OLSON, G. DAy ON TEST Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 Lab: NCTR II ~ II 6I6I6I6I6I 6I6 1 6 6 6 6 6 6 6 I 00 0 ··············································1·················································································································· C57BU6N XC3H/HEN MTV-NCTR MICE MALE 185 PPM Acryl M ANIMAL ID 0 0 0 0 0 0 0 0 0 0 0 0 3 1 213 3 3 3 4 4 1 0 s s s s s s s s I I 4 I 414 2 3 * TOTALS 4 NONE GENITAL SYSTEM Epididymis + Preputial Gland + Prostate + Seminal Vesicle + Testes + 1 1 + + + + + + + 8 HEMATOPOIETIC SYSTEM Bone Marrow + 1 lymph Node, Mandibular + 1 Lymph Node, Mesenteric + 1 Spleen + Thymus + 1 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 18 TOMS No. 20313- 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-0AY ACRYLAMIOE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1 /NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 185 PPM Acryl M 0 2 1 1 0 6 1 11 I , , 0 0 0 0 s I6 6 16 0 0 5 3 0 0 5 3 0 0 5 3 0 0 5 3 0 0 5 4 3 4 1 , 2 , 0 1 0 6 6 0 0 5 4 0 0 5 4 0 0 5 4 2 3 4 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08102/04 / 08102104 Lab:NCTR * TOTALS INTEGUMENTARY SYSTEM Mammary Gland M Skin + 0 MUSCULOSKELETALSYSTEM Bone, Femur + Skeletal Muscle + + + + + + + 8 + NERVOUS SYSTEM Brain, Brain Stem + 1 Brain, Cerebellum + 1 Brain, Cerebrum + Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 19 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1 /NCTA Pathologist: OLSON, G. DAY ON TEST C578U6N XC3H/HEN MTV·NCTR MICE MALE ANIMAL ID 185 PPM Acryl M Spinal Cord, Thoracic 11 0 1 0 1 , 0 0 6 01 6 6 6 6 6 0 0 5 3 0 0 0 0 0 0 0 5 3 5 3 5 4 0 0 5 4 1 2 3 4 5 4 1 0 0 5 4 2 3 4 + + + + + + + + 0 2 1 1 0 6 0 0 5 3 110 0 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 Lab:NCTR *TOTALS 8 RESPIRATORY SYSTEM 1 Lung + Nose + Trachea + 1 Eye + 1 Harderian Gland + 1 Kidney + 1 Urinary Bladder + 1 SPECIAL SENSES SYSTEM URINARY SYSTEM SYSTEMIC LESIONS M .. Missing !issue A .. Autotysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 20 P04: NEOPLASMS BY INDIVIDUAL ANIMAL ACRYLAMIDE CAS Number: 79-06-1 Pathologist : OLSON, G. TUMS No. 20313 - 04 Test Type: 90-DAY Route: DOSED FEED Species/Strain: MICE/86C3F1/NCTR 110 ,o , ,o I ,0 I DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 185 PPM Acryl M 02 1 0 0 6 6 6 6 6 6 00 1 0 0 0 0 5 0 0 5 3 4 0 0 5 4 1 o.o 5 5 3 1313 2 3 • • • •••000 00 000 0 Multiple Organ ,, + + 00• • • •000•0 0 + + 05 1 5 0 4 14 2 3 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02104 / 08/02104 Lab: NCTR 1 0 6 0 0 5 4 4 * TOTALS o0000•00000 0 00000000000000000000000000000•00 0 00000000000000000000 0000.0 0000 000 0000000000..0000 0 + + + 0000000000000000000000 00 0000000000000000 0 000000000000 0000000000H 00000 0000 00 + 8 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 21 P04 : NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 04 Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06·1 Species/Strai n: MICEJB6C3F1/NCTR Pathologist: OLSON, G. 1 0 a6 a6 6 2 1 1 0 0 0 6 6 6 0 0 0 0 10 0 0 4 9 4 .4 9 9 1 2 314 Esophagus + + + Gallbladder + + Intestine Large, Cecum + Intestine Large, Colon + DAY ON TEST 1 I Date Report Reqsted: 07/31/2006 Time Report Reqsted : 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 Lab: NCTR 1 1 0 0 6 6 0 0 0 0 5 0 0 5 0 5 0 0 0 0 1 2 3 4 + + A + + 7 + + + A + + 7 + + + + A + + 7 + + + + A + + 7 Intestine Large, Rectum +++++M++ 7 Intestine Small, Duodenum + + + + + A + + 7 Intestine Small, Ileum + + + + + A + + 7 Intestine Small, Jejunum + + + + + A + + 7 Liver + + + + + A + + 7 Pancreas + + + + + A + + 7 Salivary Glands + + + + + A + + 7 Stomach, Forestomach + + + + + A + + 7 C57BL/6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 370 PPM Acryl M 0 4 9 0 5 * TOTALS ALIMENTARY SYSTEM • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present .. Insufficient tissue Page 22 TOMS No. 20313 • 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-0AY Date Report Reqsted: 07/31/2006 ACRYLAMIDE Route: DOSED FEED Time Report Reqsted: 12:29:56 CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTA First Dose M/F: 08/02/04 / 08/02104 Pathologist: OLSON, G. DAY ON TEST C57BL16N XC3H/HEN ~; · 1 1 1 1 1 0 0 6 6 0 6 0 6 0 16 6 0 2 1 0 1 0 6 1 · ·~ 6 ·-~ 0 4 Lab: NCTR ···· ·· · ·· ··· ·· ·· · · · · · · ·· 0 4 9 4 0 0 0 1 0 4 9 3 0 19 0 4 9 2 0 1 0 2 0 3 0 4 Stomach, Glandular + + + + + A + + 7 Tongue + + + + + + + 8 Blood Vessel + + + + + A + + 7 Heart + + + + + + + + 8 Adrenal Cortex + + + + + A + + 7 Adrenal Medulla ·······················-················ + + + + + A + + 7 MICE MALE ANIMAL ID 370 PPM Acryl M s s s s + * TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM .......... Parathyroid Gland + + + + + A + + ..................................................... + + + + M A + + Pituitary Gland + + + Thyroid Gland + + + Islets, Pancreatic 7 6 ····················--········· + + A + 7 + ············································································································································································· + + A + + 7 GENERAL BODY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 23 TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Tesl Type: 90-DAY ACRYLAMIDE CAS Number: 79-06-1 Route: DOSED WATER Species/Slrain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST 0 0 0 9 9 9 9 9 9 9 0 9 9 0 0 0 0 0 0 0 0 0 0 0 0 9 0 0 9 9 9 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07/12104 lab: NCTR 0 9 9 ........................ ..... ........................................................................................... 0 0 0 0 1 1 1 2 Stomach, Forestomach + Stomach, Glandular Tongue C57BLJ6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID I 0 o o o o o o o o 11 1 3 4 2 1 2 2 2 3 2 4 + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 Blood Vessel + + + + + + + + 8 Heart + + + + + + + + 8 Adrenal Cortex + + + + + + + + 8 Adrenal Medulla + + + + + + + + 8 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland ++M+++++ 7 Pituitary Gland ++ +M++++ 7 Thyroid Gland + + 8 3.52 Acryl M * TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM + + + + + + • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade + .. Tissue examined microscopically M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation BLANK .. Not examined microscopically I .. Insufficient tissue Page 15 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TDMS No. 20313 · 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE CAS Number: 79-06-1 Route: DOSED WATER Spe<:ies/Strain: MICE/B6C3F 1/NCTA I 0 9 9 ~;· · -·~ - I I I9I 0 9 0 9 0 0 9 9 9 9 9 · ~· · ·~ -~ Lab: NCTR 0 I0 0 91 9 I9 9 9 9 · · ~· r 0 0 0 0 0 0 1 1 1 2 1 3 1 4 2 1 2 2 Epididymis + + + + + Preputial Gland + + + + Proslate + + + + + + 2 Bone Marrow + ~ · ·~ 0 · · ·· · · · ···· · · · ·· · · · ·· · ·· 0 o o o o o o o o MICE MALE 3.52 Acryl M First Dose M/F: 07/12/04 / 07/12/04 Pathologist: OLSON, G. DAy ON TEST C57BU6N XC3H/HEN Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:1 5:54 ANIMAL ID 212 *TOTALS 3 4 + + + 8 + + + + 8 + + + + + 8 + + + + + + 8 + 1 + 1 + + 2 + + + 1 8 1 + + + + + + + 8 Lymph Node, Mandibular M + + + + + + + 7 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 GENERAL BODY SYSTEM NONE GENITAL SYSTEM Scr.! r: ~I Vesicle Testes Germinal Epithelium, Degeneration 6 1.3 HEMATOPOIETIC SYSTEM • + x I .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade .. Tissue examined microscopically M .. Missing tissue .. Lesion present A .. Autolysis precludes evaruation .. Insufficient !issue BLANK .. Not examined microscopically Page 16 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked 20313- 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL TOMS No. Test Type: 90-DAY Route: DOSED WATER ACRYLAMIDE CAS Number: 79-06-1 ·Pathologist: OLSON, G. Species/Strain: MICE/B6C3F1/NCTR DAY ON TEST 09 109 109 109 109 109 109 -0 9 9 9 9 9 9 9 9 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07/12104 Lab: NCTR ..............................f.............................. .................................................. C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID o0 o0 o0 o0 o0 o0 o0 o0 o o o o o o o o 1 1 1 2 2 2 2 3 4 1 2 3 + + + + + + + Mammary Gland M M M M M M M M Skin + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle Atrophy + 2 + + + 1 + + + + 8 Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Thymus 1 I I1 3.52 Acryl M 2 4 + •TOTALS 8 INTEGUMENTARY SYSTEM 0 MUSCULOSKELETALSYSTEM 3 1.3 1 NERVOUS SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade + .. Tissue examined microscopically M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically Page 17 14 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICEJB6C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST 90 9 0 9 1 90 1 90 1 90 1 90 1 09 1 90 9 9 9 9 9 9 9 0 0 0 0 0 0 0 0 0 0 0 I 00 0 0 l3 4 1 2 3 4 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07/12/04 Lab: NCTR ..........-·.................·r..·· 1·......... .............·1··............................-·.·--.....-........................................................ C57BLJ6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 3.52 Acryl M 0 o o o o o o o o 1 1 j 1 1 2 2 2 2 1 Axon, Degeneration 1 Spinal Cord, Cervical + Spinal Cord, Lumbar Axon, Degeneration + Spinal Cord, Thoracic 1 2 * TOTALS 1 + + + + + 111 + 1 + + + + Lung + + Nose + Trachea 8 1.0 + + + 8 + 11 + + 8 + + + + + 8 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 Eye + + + + + + + + .8 Harderian Gland + + + + + + + + 8 + 8 1.0 11 RESPIRATORY SYSTEM SPEC!:!\ L SENSES SYSTEM URINARY SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 18 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked m MS No. 20313. 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER First Dose M/F: 07/12/04 / 07/12104 Lab: NCTR Pathologist: OLSON, G. 0 9 9 DAY ON TEST C57BLJ6N XC3H/HEN MICE MALE Time Report Reqsted: 08:15:54 CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTA 0 9 9 0 9 9 0 9 9 90 1 09 9 9 0 9 9 0 9 9 Date Report Reqsted: 06/2912006 ·························-····· ........................................................... . o o o oo oo oo oo oo MTV-NCTR 0 0 0 o o o o o o o o l 11 1 1 1 2 2 2 3 4 1 I2 2 1 3 4 Kidney + + + + + + + + 8 Urinary Bladder Lumen, Dilatation + + + + + + + + 8 4 4 4 3 4 4 4 4 3.52 Acryl M ANIMAL ID 2 *** *TOTALS 8 3.9 END OF MALE DATA *** • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 19 1-4 .. Lesion qualffied as: 1) Minimal 3) Moderate 4) Marked 2) Mild TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07112104 / 07112104 Lab: NCTR - DAY ON TEST 1111111 1 111 11 0 0 0 10 10 10 0 0 ··················-·····-····-····..............1.....r···· .................................................................................................. 0 0 0 0 0 0 0 0 C57BU6N XC3H/HEN MTV-NCTR o o o o o o o o 0 0 0 0 0 0 0 0 MICE FEMALE 0 0 0 0 0 0 0 0 7 7 7 7 8 8 8 8 12341234 Control Water F ANIMAL ID I I •TOTALS ALIMENTARY.SYSTEM Esophagus + + + + + + + + 8 Gallbladder + + + + + + + + 8 Intestine Large, Cecum + + + + + + + + 8 Intestine Large, Colon + + + + + + + + 8 Intestine Large, Rectum + + + + + + + + 8 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum 8 + + + + + + + + ........................................................................................................................................................................................................... Intestine Small, Jejunum + Liver + + + + + + + + 8 + + + + + + + 8 .............................................................................................................................................................................. • 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 • 0 0 4 0 0 0 o o o o o ooo o o o o o o o o O o o o o o • O O O OO OOOO Oooo o o o o o oo o 0 • 0 0 00 0 0 U O O o O o o o o o o o o o • o o o o o o o o o o o o o o o o o o o o o o o o o o o 0 00 • 0 0 0 0 0 0 0 o 0 • U O o o o o o o o o o o o o o o • • o o o oo o o o o o o o o o o o o oo• Pancreas + + + + + + + + 8 ·················································································································-····················································· Sahvary Glands Stomach, Forestomach + + + + + + + + 8 + + + + + + + + 8 ....................................................................................................................................................................................... • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue + .. Tissue examined microscopically x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically Page 20 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/St rain: MICE/86C3F1/NCTR First Dose M/F: 07/12/04/ 07/12/04 Lab: NCTR Pathologist: OLSON, G. 1 DAVON TEST C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID Control Water F 1 o0 I o0 0 0 0 7 1 0 0 0 7 2 1 0 1 0 0 0 0 0 0 7 3 1 0 0 0 10 0 0 o7 Io8 1 0 0 1 0 0 0 0 0 o0 Io0 8 4 1 2 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 1 0 0 T 8 8 3 4 - *TOTALS 8 Stomach, Glandular + + + + + + + + Tu::g:.::> + + + + + + + + 8 Blood Vessel + + + + + + + + 8 Heart Mineralization + + + + + + + + 8 CARDIOVASCULAR SYSTEM 1 1.0 1 ENDOCRINE SYSTEM 8 Adrenal Cortex Subcapsular, Hyperplasia 11111111 Adrenal Medulla + + + + + + + + 8 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland +++M++++ 7 Pituitary Gland + + + + + + + + 8 Thyroid Gland + + + + + + + + 8 + + + + + + + + 8 1.0 • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 21 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED WATER ACRYLAMIDE GAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTR ·Pathologist: OLSON, G. DAy ON TEST I 1 11 1 1 11 11 0 0 0 0 0 0 0 0 0 0 1 0 0 Date Report Reqsted: 06/29/2006 Time Report Reqst.ed: 08:15:54 First Dose M/F: 07/12/04 / 07/12/04 Lab: NCTR I I 1 0 0 ·1 0 0 -········-·····-···-·-······-·....................1··-·- ...................................................................................................... C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID Control Water F o0 o0 o0 o0 o0 o0 o0 o0 o o o o o o o o 7 7 7 7 8 1 2 3 4 1 8 I2 8 8 3 4 * TOTALS GENERAL BODY SYSTEM NONE GENITAL SYSTEM Clitoral Gland +++M++++ Ovary + + + + + + + + 8 Uterus + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular + + + + + + + + 8 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + + + + 8 7 HEMATOPOIETIC SYSTEM INTEGUMENTARY SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade + .. ii;;s;,;3 examined microscopically M •. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically Page 22 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20313 · 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE CAS Number: 79-06-1 Route: DOSED WATER Pathologist: OLSON, G. Species/Strain: MICEJB6C3F1/NCTR Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 Fi rst Dose M/F: 07/12104 / 07/12/04 Lab: NCTR 1 DAY ON TEST C57BU6N XC3H/HEN ~ -· 1 11 11 11 0 0 0 0 0 0 0 0 . r ~ -~ · ·-~ ·~- I 0 0 7 0 Mammary Gland Skin 1 1 1 11 0 10 10 10 0 0 0 0 - ~ - r ~· r ~· ·~ 0 0 0 0 0 7 0 0 7 0 0 0 0 + + + + + + + + 8 + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 MICE FEMALE ANIMAL ID Control Water F I 0 0 7 8 8 8 8 12341234 *TOTALS MUSCULOSKELETAL SYSTEM NERVOUS SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 23 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIOE CAS Number. 79-06-1 Route: DOSED WATER Species/Strain: MICEJB6C3F1 /NCTR Pathologist: OLSON, G. DAY ON TEST 1 1 1 1 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 C57BU6N XC3H/HEN ~: ~ ~ .. MICE FEMALE AN IMAL ID Control Water F .. g·rg··g·· 0 7 1 1 0 7 2 I Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12104 I 07112104 Lab:NCTR 1 i ig-··g·..g·r·g····· ···························· 0 7 3 0 7 4 I 80 0 0 0 8 8 8 1 2 3 4 *TOTALS RESPIRATORY SYSTEM Lung + + + + + + + + 8 Nose + + + + + + + + 8 Trachea + + + + + + + + 8 Eye + + + + + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 SPECIAL SENSES SYSTEM URINARY SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade · M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation BLANK .. Not examined microscopically I .. lnsutticient tissue + .. Tissue examined microscopically Page 24 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20313- 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED WATER ACRYLAMIDE CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLS0N, G. DAy ON TEST Date Report Reqsted: 06/29/2006 nme Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07112/04 lab: NCTR I 1111111 111' 0 0 0 0 0 1, 0 0 I0 0 0 0 0 0 0 0 10 ······························r···· ·········· .......... ····1·································································································· C57BlJ6N XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 o0 o0 o0 1 1 1 MICE FEMALE 0.35 Acryl F ANIMAL ID 5 1 5 2 5 3 1 5 4 1 6 1 1 6 2 1 6 3 I1 6 4 " TOTALS ALIMENTARY.SYSTEM Liver Mineralization + 1 1 + 1 1 1.0 CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE: GENITAL SYSTEM Ovary Cyst x 1 HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM NONE • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation BLANK .. Nol examined microscopically I .. Insufficient tissue + .. Tissue examined microscopically Page 25 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL ACRYLAMIDE Test Type: 90·DAY Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST Date Report Reqsted: 06/29/2ooG Time Report Reqsted: 08:1 5:54 First Dose M/F: 07112104 / 07112104 Lab: NCTR 11111 111 111 0 0 0 0 0 0 11 0 o o o o l o o o o 1 0 ..............................r··-· ...............r....r·························..·················································..········-..·············· C578U6N XC3H/HEN MTV-NCTR MICE FEMALE 0.35 Acryl f ANIMALID 0 0 0 0 0 0 0 0 1 5 1 5 1 5 1 5 1 2 3 4 0 0 0 0 1 1 6 1 6 2 0 0 I 0 0 II 6 3 6 4 * TOTALS MUSCULOSKELETALSYSTEM NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically _ + .. Tissue examined microscopically Page 26 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 4) Marked 2) Mild TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED WATER ACRYLAMIDE CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR .Pathologist: OLSON, G. I DAy ON TEST 1 0 0 1 0 0 1 0 0 0 0 1 9 1 0 0 1 9 2 0 0 1 9 3 1 0 0 1 0 0 0 0 1 9 4 0 0 2 0 1 I 1 0 0 11 0 0 2 0 2 0 0 2 0 3 0 0 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose MJF: 07/12/04 I 07112104 Lab: NCTR. I ·1 0 0 .............................. ····1···········-·········r···· .................................................................................................. C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 0.70 Acryl F I I 1 I 0 0 2 0 4 * TOTALS ALIMENTARY SYSTEM NOOE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM NONE HEMATOPOIETIC SYSTEM Spleen Pigmentation + 2 1 1 2.0 INTEGUMENTARY SYSTEM NONE • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade + .. Tissue examined microscopically M .. Missing tlssue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically Page 27 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 4) Marked 2) Mild TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACAYLAMIDE CAS Number: 79-06-1 Pathologist: OLSON, G. Roule: DOSED WATER Species/Strain: MICEJ86C3F1/NCTR DAy ON TEST · MICE FEMALE 0.70 Acryl F ANIMAL ID First Dose M/F: 07112104 / 07/12/04 Lab: NCTR 11111 0 0 0 I 111111111 0 0 0 0 0 0 C57BU6N XC3H/HEN ~;· Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 0 · 1 · · ·~ · ·~ I 0 0 0 ·~ · · · ~· 0 ·~ 0 0 · ·~ r ~ 0 2 0 . . . . . . . ... . . . . . 0 1 9 0 1 9 0 1 9 0 1 9 0 2 0 0 2 0 0 2 0 1 2 3 4 1 2 I3 + + + + + + + + 8 + + + + + + + + 8 * TOTALS 4 MUSCULOSKELETALSYSTEM Skeletal Muscle NERVOUS SYSTEM Peripheral Nerve RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM Eye Lens, Developmental Malformation + 1 x 1 URINARY SYSTEM NONE • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue A .. Autotysis precludes evaluation x •. Lesion present I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 28 1·4 .. Lesion qualified as: 1) Minimal 3) Moderate 4) Marked 2) Mild P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 02 Test Type: 90-DAY A9RYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 1.41 Acryl F 01 1 01 1 01 1 01 1 01 1 01 1 1 o o o, olo o 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 1 2 1 1 2 0 10 1 1 2 2 3 4 1 + + + + + Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07/12/04 lab: NCTR 1 2 13 2 4 *TOTALS + + 8 ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCmNESYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM Ovary + HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM $1cin 1 + • .. Total animals with tissue examined microscopically: Total animals with lesion and mean severity grade + .. Tissue examined microscopically M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation BLANK .. Not examined microscopically I .. Insufficient tissue Page 29 1-4 ... Lesion qualified as; 1) Minimal 3) Moderate 2) Mild 4) Marke<:!· TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS Test Type: 90-DAY BY INDIVIDUAL ANIMAL ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICEJB6C3F1/NCTR Pathologist: OLSON, G. 0 · ·~ 0 0 0 0 0 0 0 0 1 11 1 1 3 1 4 2 1 1 1 2 12 2 3 2 4 r -~· . 0 0 0 O•O 0 MICE FEMALE First Dose M/F: 07/12104 /07/12/04 Lab: NCTR 1111111 1 1 1\ 111 0 0 10 0 0 0 0 0 DAY ON TEST C57BU6N XC3H/HEN ~;· Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 ~· 0 r ~· 1 1 1 1 1 I2 + + + + + + + + 8 Peripheral Neive + + + + + + + + 8 Spinal Cord, Ceivical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 ANIMAL ID 1.41 Acryl F 1 *TOTALS MUSCULOSKELETALSYSTEM SkP.letal Muscle NERVOUS SYSTEM RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade + .. Tissue examined microscopically M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically Page 30 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TDMS No. 20313 • 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. 01 0 1 01 0 0 0 0 3 1 3 2 Esophagus + Gallbladder DAY ON TEST 10 0 11 0 1 1 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose MJF: 07/12/04 / 07/12104 Lab: NCTR 1 0 0 1 0 0 o Io 0 0 0 .............................. ···· 1··········0 ................................................................................................................. I o o I 0 0 0 0 0 0 0 0 0 0 3 3 3 4 4 1 4 2 4 3 4 4 + + + + + + + 8 + + + + M + + + 7 Intestine Large, Cecum + + + + + + + + 8 Intestine Large, Colon + + + + + + + + 8 Intestine Large, Rectum + + + + + + + + 8 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 liver Tension lipidosis + + + + + + + + 8 2 Pancreas + + + + + + + + 8 Salivary Glands + + + + + + + + 8 Stomach. Forestomach + + + + + + + + 8 C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE 3.52 Acryl F ANIMAL ID o o 0 0 o o o o *TOTALS ALIMENTARY SYSTEM • + x I 1 2.0 .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade .. Tissue examined microscopically M .. Missing tissue A .. Autolysis precludes evaluation .. L'.lsior:i present .. Insufficient tissue BLANK .. Not examined microscopically Page 31 1-4 .. l esion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20313 • 02 Test Type: 90-DAY P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Reqsted: 06/29/2006 ACRYLAMIDE CAS Number: 79-06-1 Time Report Reqsted: 08:15:54 First Dose M/F: 07112/04 / 07/ 12/04 Route: DOSED WATER Species/Strain: MICE/86C3F1/NCTR 'Pathologist: OLSON, G. DAy ON TEST I 1 11 0 0 0 0 1 0 0 1 0 0 11 0 0 1 0 0 II , 0 0 Lab: NCTR ·1 0 0 ...............................f........................ ................................................................................ C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE o0 o0 o0 o0 o0 o0 o0 o0 o o o o o o o o . 31313 3 1 2 3 4 4 1 4 2 s 4 4 4 Stomach, Glandular + + + + + + + + 8 Tongue + + + + + + + + 8 ++ +++ 7 3.52 Acryl F ANIMAL ID * TOTALS CARDIOVASCULAR SYSTEM Blood Vessel +M+ Heart + + + + + + + + 8 Adrenal Cortex Subcapsular, Hyperplasia + 1 + + 1 + 1 + 1 + 1 + 1 + 2 8 1 Adrenal Medulla + + + + + + + + 8 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland + +M+ + + ++ 7 Pituitary Gland M + + + + + 7 Thyroid Gland ++M+ ++ 7 ENDOCRINE SYSTEM • + x I + + ++ .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade .. nssue examined microscopically M .. Missing tissue .. Lesion present A .. Autolysis precludes evaluation .. Insufficient tissue BLANK .. Not examined microscopically Page 32 8 1.1 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TDMS No. 20313 · 02 Test Typ<?: ~0-DAY P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Pathologist: OLSON, G. Species/Strain: MICE/B6C3F1/NCTR 1 0 0 DAVON TEST 1 0 0 1 0 0 1 0 0 1 0 0 1 0 0 1 0 0 1 0 0 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 Fi rst Dose M/F: 07112/04 / 07/12/04 lab: NCTR .............................. -- ··1··········1·········· ..................................................................................................... . I 0 0 0 0 0 0 0 0 0 0 00 0 0 00 I 3 ;1 3 2 3 3 3 4 4 1 4 2 4 3 4 4 Clitoral Gland + + + + + + + + 8 Ovary + + + + + + + + 8 C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 3.52 Acryl F o o o o o o o o ! * TOTALS GENERAL BODY SYSTEM NONE GENITAL SYSTEM An estrus x x x x x x 6 + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular M + + + + + + + 7 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + +· + + + + 8 Thymus + + + + + + + 8 Uterus HEMATOPOIETIC SYSTEM + INTEGUMENTARY SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 33 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Mar!(ed TOMS No. 20313 • 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Reqsted: 06/29/2006 Test Type: 90-DAY Route: DOSED WATER ACRYLAMIDE Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07112/04 CAS Number: 79-06·1 Species/Strain: MICE/86C3F1 /NCTR Pathologist: OLSON, G. DAY ON TEST Lab: NCTR 1 1 01 01 1 01 1 01 1 01 1 01 1 01 1 0 0 0 0 0 0 0 0 0 ........................,..... ..............·1·.. ·1·.......................................................................................................... C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID o0 o0 o0 o0 o0 o0 o0 o0 o o o o o o o o 3 1 3 2 3 3 3 14 1 , 2 4 3 4 4 Mammary Gland + + + + + + + + 8 Skin + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle Atrophy + + + + + + 2 + 1 + , 8 1 3.52 Acryl f 4 4 * TOTALS MUSCULOSKELETAL SYSTEM 1 5 1.2 NERVOUS SYSTEM • + x I Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve Axon, Degeneration + 1 + 1 + 1 + 2 + 1 + 2 + 1 + 2 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade .. Tissue examined microscopically M .. Missing tissue .. Lesion present A .. Aulolysis precludes evaluation .. Insufficient tissue BLANK .. Not examined microscopically Page 34 8 1.4 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Pathologist: OLSON, G. Species/Strain: MICE/B6C3F1INCTR DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 3.52 Acryl F j gl g 0 0 1 0 0 00 1 00 0 o,o 3 3 2 1 3 3 1 0 j 1 0 1 0 olo o 1 0 0 0 0 0 0 0 0 3 4 4 0 0 0 4 4 1 2 3 0 0 0 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:1 5:54 First Dose M/F: 07/12/04 / 07/12/04 Lab: NCTR 1 0 0 0 0 0 4 4 * TOTALS 7 1.0 Axon, Degeneration Spinal Cord, Thoracic + + + + + + + + 8 Lung + + + + + + + + 8 Nose + + + + + + + + 8 Trachea + + + + + + + + 8 Eye + + + + + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder Lumen, Dilatation + + + + + + + + 8 2 4 2 RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM URINARY SYSTEM • + x I 4 2.8 3 .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade .. Tissue examined microscopically M .. Missing tissue .. Lesion present A .. Aulolysis precludes evaluation .. Insufficient tissue BLANK .. Not examined microscopically Page 35 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL TPMS No. 20313 - 02 Test Type: 90-DAY ACRYLAMIDE CAS Number: 79-06-1 Route: DOSED WATER Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. DAy ON TEST Date Report Reqsted: 06/2912006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12104 / 07112104 Lab:NCTR I 01 1 10 1 01 1 01 1 01 1 01 1 01 1 01 0 0 0 0 0 0 0 0 .. .... . .. .... .... ............ . ... . , . ..... .. ....... ... ·r ...... ... ..........•......... . C57BLJ6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 3.52 Acryl F o o o o o o o o lo 0 [ 3 1 0 0 0 0 0 I 0 l 0 o o o o o o o 3 2 3 3 3 4 4 1 4 2 I 4 3 4 4 *TOTALS *** END OF REPORT *** • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 36 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL TOMS No. 20314 · 02 Test Type: 90-DAY GLYCIDAMIDE CAS Number: 5694-00-8 Route: DOSED WATER . Pathologist: OLSON, G. Species/Strain: MICE/86C3F1/NCTR C Number: C20314 Lock Date: Not Entered. Cage Range: ALL Date Range: ALL ReaSOiiS ror Removal: ALL Removal Date Range: ALL Treatment Groups: Include ALL Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07/26/04 Lab: NCTR TOMS No. 20314- 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Gl YCIDAMIOE Test Type: 90-DAY Date Report Reqsted: 06129/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07126104107126104 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR DAY ON TEST 90 I 90 I I 90 09 90 9 9 9 I 90 I90 I90 , 9 9 9 9 9 4 4 4 4 4 4 4 4 1 2 1 3 4 2 1 I2 I 2 3 2 4 Esophagus + + + + + + + + 8 Gallbladder + + + + + + + + 8 Intestine Large, Cecum + + + + + + + + 8 Intestine Large, Colon + + + + + + + + 8 Intestine Large, Rectum ++++M+++ 7 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 Liver Tension lipidosis + + + + + + + + 8 Pancreas + + + + + + + + 8 Salivary Glands + + + + + + + + 8 S:o:n:::r.h, Forestomach + + + + + + + + 8 ........................1····················· "··1 ·········· ............................................................... C57BU6N XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 o0 o0 o0 MICE MALE Control Water M ANIMAL ID 11 1 1 2 *TOTALS ALIMENTARY SYSTEM 1 2.0 2 • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 2 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314- 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-0AY GLYCIDAMIDE Route: DOSED WATER CAS Number: 5694-00-8 Species/Strain: MICEJB6C3F1/NCTA Pathologist: OLSON, G. DAy ON TEST 0 0 0 9 9 9 9 ,9 I9 0 I 9 f9 0 I I 0 0 First Dose M/F: 07/26/04 / 07/26/04 lab: NCTR 0 9 91919 9 9 9 9 ' Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 .............................. .... ·········· .......... ....................................................................................................... C57BU6N XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 o0 o0 0o . MICE MALE 4 1 4 1 4 1 Control Water M 11 4 4 4 2 1 2 2 1 2 3 Stomach, Glandular + + + + + Tongue + + + + Blood Vessel + + + + Heart + + Adrenal Cortex Accessory Adrenal Cortical Nodule M + Adrenal Medulla M + + + + + + + 7 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland + + + + + + + + 8 Pituitary Gland +++++M++ 7 Thyroid Gland + 8 ANIMAL ID 4 4 2 4 3 2 4 + + + 8 + + + + 8 + + + + 8 + + + + + + 8 + + + + + 7 *TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM + x + + 1 + + + + + .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue A .. Autolysis precludes evaluation x .. Lesion present I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 3 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqst ed: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07126104 / 07126104 Species/Strain: MICEJ86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR 1~ 0 0 0 9 9 9 9 9 9 0 0 4 1 1 0 0 4 1 0 0 2 Epididymis + Preputial Gland DAY ON TEST 9 I o9 Io9 o9 Io9 9 9 0 0 4 3 0 0 4 1 4 + + + + Prostate + Seminal Vesicle Testes 9 9 0 0 4 0 0 0 4 2 1 2 2 2 3 2 + + + + + 8 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular + + + + + + + + 8 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 .... .... .......... .... .......... ..................................... C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID Control Water M 4 1 0 4 *TOTALS 4 GENERAL BODY SYSTEM NONE GENITAL SYSTEM HEMATOPOIETIC SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 4 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 · 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Tes1 Type: 90·0AY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07126104 Species/Strain: MICE/B6C3F1 /NCTR Pathologist: OLSON, G. Lab: NCTR DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID Control Water M Thymus 9 9 s l s~ iI s~ s9 I0s9 I 0s9 010 9 9 s 0 0 0 0 4 0 2 1 3 1 4 + + + 0 0 4 1 1 0 0 4 1 + 0 4 0 0 0 1 0 4 2 2 3 0 0 4 2 4 + + + + 0 0 4 2 4 2 *TOTALS 8 INTEGUMENTARY SYSTEM Mammary Gland M M M M M M M M 0 Skin + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 MUSCULOSKELETAL SYSTEM NERVOUS SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Pages 1-4 .. Lesion qualified as: 1) Mi.nimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314- 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL GLYCIDAMIDE Test Type: 90-DAY Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: OOSEI) WATER CAS Number: 5694--00-8 First Dose MJF: 07/26/04 / 07/26/04 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR 9 C57BU6N XC3H/HEN ~; · I o9 I o9 I o9 I o9 o9 o9 o9 DAY ON TEST ·· 1 ·~ 9 -~· 9 · · ~· 1 9 · ·~ 9 · -·~ 1 9 .0 9 9 19 · · · ~· ·~ ·· ·· · · · ·· · · · · ·· · · · · ·· ·· ·· ·· 0 0 0 0 0 0 0 0 4 4 4 4 4 4 4 4 11 1 1 2 1 3 11 4 2 1 2 2 2 3 2 4 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 Lung + + + + + + + + 8 Nose + + + + + + + + 8 Trachea + + + + + + + + 8 Eye + + + + + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + + + + + + + + Urinary Bladder + + + + + +. + + MICE MALE Control Water M ANIMAL ID * TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM URINARY SYSTEM '- 8 8 • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 6 1­4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Mar1<ed TOMS No. 20314 · 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-0AY Route: DOSED WATER GLYCIOAMIDE CAS Number: 5694--00-8 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 0.14 Glycid M 09 0 19 0 1 09 1 09 9 9 9 9 9 0 1 09 1 09 9 9 9 9 9 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 3 3 7 7 7 7 1 2 3 4 3 8 , 2 3 4 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07126104 Lab:NCTR 313 8 8 3 8 *TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM • Preputial Gland Cyst 1 + 2 1 2.0 HEMATOPOIETIC SYSTEM Spleen 1 + INTEGUMENTARY SYSTEM NONE • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade + .. Tissue examined microscopically M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation BLANK .. Not examined microscopically I .. lnsutticient tissue Page7 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL TestType: 90-0 AY GLYCIDAMIDE Date Report Reqsted: 06129/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07126104 107/26104 Species/Strain: MICE/B6C3F1 /NCTA Pathologist: OLSON, G. Lab: NCTR DAY ON TEST C57BU6N XC3H/HEN MICE MALE I I I o9 I o9 o9 o9 I o9 o9 o9 o9 9 9 9 3 72 3 3 19 9 9 9 9 3 3 3 3 8 8 3 84 ................................................................................................................................................................. MTV-NCTR o0 o0 1. o0 o0 o0 o0 o0 0O ANIMAL ID 0.14 Glycid M 7 1 7 3 718 4 1 2- 3 * TOTALS MUSCULOSKELETALSYSTEM NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present . A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 8 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 Test Type: 90-DAY P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Rout e: DOSED WATER CAS Number: 5694-00-8 Pathologist: OLSON, G. GLYCIDAMIDE Species/Strain: MICE/86C3F1/NCTR DAY ON TEST Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07/26/04 Lab: NCTR 09 0!01010101010 9 9 9 9 9 9 9 9 9 19 9 9 9 9 9 4 5 4 5 4 5 1 2 4 6 1 4 6 2 4 14 6 6 3 4 .......................... .... .......................................................... ....... C57BLJ6N XC3H/HEN MTV-NCTR 0o 0o 0o 0o 0o o0 0o o0 MICE MALE ANIMAL ID 0.35 Glycid M 4 5 3 4 * TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM Preputial Gland Duct, Dilatation + 2 1 2.0 HEMATOPOIETIC SYSTEM NONE INTEGUMENTARV SYSTEM Skin Inflammation, Chronic Active 1 + 1 3.0 3 • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue A .. Autolysis precludes evaluation x .. Lesion present BLANK .. Not examined microscopically I .. Insufficient tissue + .. Tissue examined microscopically Page 9 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIOE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Rout.e : DOSED WATER CAS Number: 5694-00-8 First Dose MIF: 07/26/04 / 07/26/04 Species/St rain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE MALE . ANIMAL ID 0.35 Glycid M Fat, .~acrosi I I o9 o9 o9 9 I9 9 010 9 9 1010 9 9 0 9 9 9 9 9 9 0 0 4 5 1 0 0 0 0 4 6 1 0 0 4 0 0 0 4 6 3 4 6 4 0 0 4 5 2 0 0 4 5 3 4 5 4 6 2 0 *TOTALS 1 2.0 2 MUSCULOSKELETALSYSTEM NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE • + x I .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade .. Tissue examined microscopically M .. Missing tissue .. Lesion present A .. Autolysis precludes evaluation .. Insufficient tissue BLANK .. Not examined microscopically Page 10 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL TOMS No. 20314 - 02 Test Type: 90-DAY GLYCIDAMIDE Route: DOSED WATER CAS Number: 5694-00-8 Species/Strain: MICE/86C3F1/NCTR · Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN ~ -~ · MICE MALE ANIMAL ID 1.41 Glycid M l o Io Io 9 9 r ~ · ·~ 9 9 0 2 0 2 I1 5 + ·~ 9 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07126/04 / 07/26/04 Lab: NCTR I o9 o9 I o9 o9 ·o9 9 0 2 0 2 5 2 5 3 + + 9 9 · ·~ r ~· · ·~ 9 9 0 0 2 0 2 0 2 0 2 5 4 6 1 6 2 6 3 6 4 *TOTALS + + + + + 8 ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM Testes HEMATOPOIETIC SYSTEM NONE INTEGUMENTARV SYSTEM NONE MUSCULOSKELETALSYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically x .. Lesion present Page 11 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-0AY GLYCIDAMIOE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07/26/04 Species/St rain: MICE/86C3F1 /NCTR Pathologist: OLSON, G. Lab: NCTR DAY ON TEST 01010 9 9 9 0 9 0101010 9 9 9 9 9 C578U6N XC3H/HEN ~; ·~- MICE MALE 1.41 Glycid M ANIMAL ID 0 2 5 1 9 9 9 · ~ · ·~ · · 1 ·~ 1 ·~ 0 2 0 2 5 2 5 3 1 9 0 2 0 2 5 4 6 1 I I 9 9 9 . ·~ r · ~ · o 0 2 0 2 6 2 6 3 0 2 6 4 * TOTALS NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue A .. Autolysis precludes evaluation x .. Lesion present BLANK .. Not examined microscopically I .. Insufficient tissue + .. Tissue examined microscopically Page 12 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 4) Marked 2) Mild TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GL.YCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07/26/04 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR DAy ON TEST lo ,' o l o l o l o l o l o Io 9 9 9 9 9 9 9 0 0 0 0 0 0 0 0 3 3 1 3 3 2 3 3 3 3 3 4 3 4 1 3 4 2 3 4 3 3 4 4 Esophagus + + + + + + + + 8 Gallbladder + + + + + + + + 8 Intestine Large, Cecum + + + + + + + + 8 Intestine Large, Colon + + + + + + + + 8 Intestine large, Rectum + + + + + + + + 8 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 liver + + + + + + + + 8 Pancreas + + + + + + + + 8 Salivary Glands + + + + + + + + 8 Stomach, Forestomach + + + + + + + + 8 C57BLJ6N XC3H/HEN ~; ·~ MICE MALE ANIMALID 3.52 Glycid M 9 9 9 · ~· 9 9 9 9 · ·~ r ~· · ·~ I 9 9 ·~ *TOTALS ALIMENTARY SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 13 1·4 .. l esion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GlYCIDAMIDE Date Report Reqsted: 06/29/2006 Route: DOSED WATER CAS Number: 5694-00-8 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 I 07/26104 Species/Strain: MICEJB6C3F1INCTR Pathologist: OLSON, G. Lab : NCTR DAVON TEST 09 010 9 9 9 9 9 3 3 3 1 3 2 Stomach, Glandular + Tongue 0 1 0 11 0 1 0 9 19 9 9 0 9 9 9 9 9 9 3 3 3 3 3 3 3 3 3 4 4 1 4 2 4 3 4 4 + + + + + + + 8 + + + + + + + + 8 Blood Vessel + + + + + + + + 8 Heart + + + + + + + + 8 Adrenal Cortex + + + + + + + + 8 Adrenal Medulla + + + + + + + + 8 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland +++M+M++ 6 Pituitary Gland + + + + + + + + 8 Thyroid Gland + + + + + + + + 8 ................................... ··········1··············· C57BU6N XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 o0 o0 o0 MICE MALE ANIMAL ID 3.52 Glycid M * TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM GENERAL BODY SYSTEM • .. Total animalS with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. l esion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 14 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Mar1<ed TOMS No. 20314- 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Dale Report Reqsted: 06/29/2006 GLYCIDAMIDE Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07/26/04 Test Type: 90-DAY Route: DOSED WATER CAS Number: 5694-00-8 Pathologist: OLSON, G. Species/Strain: MICE/86C3F1/NCTR DAY ON TEST 0 0 9 9 9 9 o C57BU6N XC3H/HEN MTV-NCTR MICE MALE ·~ olo 0 0 0 0 9 19 9 9 9 9 9 9 9 9 9 · ·~ ·~ r 9 ~· · -~· Lab: NCTR ·~ 0 0 0 0 0 0 3 3 1 3 3 2 3 3 3 13 3 3 4 4 3 14 11 2 3 4 3 3 4 4 Epididymis + + + + + + + + 8 Preputial Gland + + + + + + + + 8 Prostate + + + + + + + + 8 Seminal Vesicle + + + + + + + + 8 Testes Germinal Epithelium, Degeneration + , + 2 + 2 + 2 + 2 + 1 + + 2 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular + + + + + + + + 8 Lymph Node. Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + + + + 8 ANIMAL ID 3.52 Glycid M 0 0 *TOTALS NONE GENITAL SYSTEM 7 1.7 HEMATOPOIETIC SYSTEM "* •• Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically Page 15 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Miid 4) Marked TOMS No. 2031 4 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Reqsted: 06/29/2006 GLYCIDAMIDE Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07/26/04 Test Type: 90-DAY Route: DOSED WATER CAS Number: 5694-00-8 . Pathologist: OLSON, G. Species/Strain: MICE/86C3F1/NCTR 0 DAY ON TEST I~ 9 I I 9 9 9 9 9 9 9 ' 9 Lab: NCTR o o o o o I o ·o 919 9 9 9 ························"l····· .............................. ·································································································· C57BU6N .XC3H/HEN MTV-NCTR MICE MALE 0 0 3 0 0 0 0 0 0 o o o o o o o o 3 3 3 3 3 3 3 3 3 1 12 3 3 3 4 4 1 4 2 4 3 4 4 Mammary Gland M M M M M M M M 0 Skin + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve Axon, Degeneration + + + + , + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 ANIMAL ID 3.52 Glycid M * TOTALS INTEGUMENTARY SYSTEM MUSCULOSKELETAL SYSTEM NERVOUS SYSTEM 8 ;~:. ~rain 1 1.0 • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Nol examined microscopically + .. Tissue examined microscopically Page 16 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TDMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIDE Route: DOSED WATER CAS Number: 5694-00-8 Pathologist: OLSON, G. SpecieS/Strain: MICE/B6C3F1 /NCTR 0 DAY ON TEST I 9 o9 o9 0 9 90 1 09 9 9 9 9 I 9 9 0 9 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07126104 Lab: NCTR 0 9 9 ······························ " ····r ····1················ ····1·································································································· c57-· :SN XC3H/HEN MTV-NCTR o~ MICE MALE oo oo o0 1 o0 oo oo oo oo I 3 3 3 3 1 3 2 3 3 Spinal Cord, Lumbar + + Spinal Gord, Thoracic + Lung Inflammation, Chronic Active + Nose + + + + + + + + 8 Trachea + + + + + + + + 8 Eye + + + + + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 3.52 Glycid M ANIMAL ID 3 3 3 3 3 3 4 4 1 2 3 4 + + + + + + 8 + + + + + + + 8 + + + + + + + 8 4 414 *TOTALS RESPIRATORY SYSTEM 1 2.0 2 SPECIAL SENSES SYSTEM URINARY SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade + .. nssue examined microscopically M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically Page 17 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 • 02 Test Type: 90-0AY Route: DOSED WATER P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL G~ YCIDAMIDE CAS Number: 5694-00-S Pathologist: OLSON, G. Species/Strain: MICE/B6C3F1 /NCTR DAY ON TEST C57BU6N XC3HIHEN MTV-NCTR MICE MALE ANIMAL ID 3.52 Glycid M Lumen, Dilatation 0 9 0 9 0 0 9 9 0 9 0 9 0 9 9 9 9 9 9 9 9 ol o 0 0 0 3 4 1 0 0 0 0 3 3 3 0 0 3 3 1 2 3 0 3 0 3 3 4 3 4 2 0 3 4 3 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07/26/04 Lab: NCTR 0 9 9 0 0 3 4 4 *TOTALS 1 4.0 4 '"** END OF MALE DATA*** • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evalualion BLANK .. Not examined microscopically I .. Insufficient tissue + .. Tissue examined microscopically Page 18 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 4} Mar1<ed 2) Mild TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Reqsted: 06/29/2006 GLYCIOAMIOE Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07/26104 l ab: NCTR Test Type: 90-0AY Route: DOSED WATER CAS Number: 5694-00-8 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. 01 0 110 1 1 0 0 01 110 0 0 0 0 0 0 0 o0 o0 o0 o0 1 4 3 2 0 0 4 3 3 3 4 41414 1 2 3 4 Esophagus + + + + + + + + 8 Gallbladder + + + + + + + + 8 Intestine Large, Cecum + + + + + + + + 8 Intestine Large, Colon + + + + + + + + 8 Intestine Large, Rectum + + + + + + + + 8 Intestine Small, DuOdenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 Liver + + + + + + + + 8 Pancreas Cyst + + + + + + + + 8 Salivary Glands + + + + + + + + 8 Stomach, Forestomach + + + + + + + + 8 DAY ON TEST C578U6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID Control Water F 01 0 0 0 4 3 110 0 0 0 4 II 4 4 4 4 * TOTALS 4 ALIMENTARY SYSTEM x 1 • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. nssue examined microscopically Page 19 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 GL YCIDAMIDE Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 I 07126104 Species/Strain: MICEJB6C3F1 /NCTR Pathologist: OLSON, G. Lab: NCTR I 01 11 0 01 DAY ON TEST 0 C57BU6N XC3H/HEN ~· · 0 · · ~ - · ·~ 0 ·~ 10 111111 0 0 0 01 0 · ·~ 0 ·~ 0 0 10 ·r ~ · · ~ · · ~ · · · ·· · ·· ··· · ····· · ··· · · ·· ··· · · · · · · · · ·· · · ·· ·· · 0 0 0 0 0 0 0 0 4 4 4 4 4 4 4 4 3 1 3 2 3 3 3 4 4 1 4 2 4 3 4 4 Stomach, Glandular + + + + + + + + 8 Tongue + + + + + + + + 8 Blood Vessel + + + + + + + + 8 Heart + + + + + + + + 8 + + + + + + 7 2 1 2 1 2 MICE FEMALE ANIMAL ID Control Water F * TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM Adrenal Cortex Accessory Adrenal Cortical Nodule Subcapsular, Hyperplasia M + Adrenal Medulla M++ +++ + + 7 + + + + + + + 8 Parathyroid Gland ++M + + + + + 7 Pituitary Gland + + + + + + + + 8 Thyroid Gland + + + + + + + + 8 Islets, Pancreatic 1 + x 1 6 1.5 ..................................................................................................................................................,........................................................ Toiai animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically v •• + .. Tissue examined microscopically Page 20 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL GLYCIDAMIDE Test Type: 90-DAY Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07/26/04 Species/Strain: MICE/86C3F1/NCTR · Pathologist: OLSON, G. Lab: NCTR DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID Control Water F 1 1 0 0 0 0 01 0 0 0 0 0 4 3 1 110 , 0 0 0 01 0 0 0 0 0 0 0 4 3 4 3 4 3 4 4 2 3 4 110 1· 01 0 0 0 0 0 1 0 4 4 2 0 4 4 3 0 4 4 4 *TOTALS GENERAL BODY SYSTEM NONE GENITAL SYSTEM Clitoral Gland +M+M + + ++ 6 Ovary + + + + + + + + 8 Uterus + + + + + + + + 8 Bone Marrow + + + + + +. + + 8 Lymph Node, Mandibular + + + + + + + + 8 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + + + + 8 HEMATOPOIETIC SYSTEM INTEGUMENTARY SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 21 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 • 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Rout e: DOSED WATER CAS Number: 5694-00-8 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose MIF: 07/26/04 I 07126104 Species/Strain: MICE/86C3F1 /NCTR Pathologist: OLSON, G. Lab: NCTR Test Type: 90-DAY GLYCIDAMIDE 1 DAY ON TEST 0 10 0 0 0 11 0 11 0 1 1 0 0 0 1 11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 4 4 4 4 4 4 4 3 1 3 3 4 4 4 2 3 3 1 12 4 3 4 4 Mammary Gland + + + + + + + M 7 Skin ++++M+++ 7 .... .. .... C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID Control Water F ·········· ·········· ........................................................................................................ •TOTALS MUSCULOSKELETALSYSTEM Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Stem + + + + + + + + 8 .- Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 NERVOUS SYSTEM • .. Total animals wilh tissue examined microscopically; Total animals wrth lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 22 1-4 .. Lel?ion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDJVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIOE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07/26/04 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR DAY ON TEST I 1 1 1 0 0 O 11 1 0 O 1 1 11 O 9 0 - ·­·- .o 0 0 0 0 0 0 0 ................................................................................................................................................................ C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE gggggggg · 4 4 4 4 4 4 4 4 3 1 3 3 2 , s I4 3 4 1 4 2 4 3 4 4 • + + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 Eye; + + + + + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 ANIMAL ID Control Water F TOTALS RESPIRATORY SYSTEM Lung Nose Trachea ......................................................................................................................................................................... ................................... SPECIAL SENSES SYSTEM URINARY SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M -- Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 23 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 nme Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07/26/04 Species/Strain: MICE/B6C3F1 /NCTA Pathologist: OLSON, G. Lab: NCTA DAy ON TE:.,T 11111'I111 I o11111 o ojo o o o o 0 0 0 0 0 0 0 0 ······························ .... ................1·····..··· ...............................................................................................-.. C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE 0.14 Glycid F ANIMALID o0 o0 o0 o0 o0 o0 o0 o0 3 9 1 3 9 2 3 9 3 3 9 4 4 0 1 I 4 0 2 4 0 3 4 0 4 * TOTALS ALIMENTARY. SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM Ovary Cyst 1 + x 1 HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM NONE • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 24 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Mar1<ed TDMS No. 20314 - 02 P09: NON­NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90·0AY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 nme Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694­00­8 First Dose M/F: 07/26/04 / 07/26/04 Species/Strain: MICE/B6C3F1/NCTA Pathologist: OLSON, G. Lab: NCTR DAY ON TEST 1 1 0 0 1 0 0 1 0 0 0 10 0 0 0 0 0 0 3 13 3 3 0 0 1 0 0 1 0 0 1 1 0 0 0 0 0 0 4 0 1 0 0 4 0 2 0 0 4 0 3 0 0 4 0 4 ......... ................................................. · C57BU6N XC3H/HEN MTV­NCTR MICE FEMALE ANIMAL ID 0.14 Glycid F 9 1 ~ 9 2 9 3 9 4 *TOTALS MUSCULOSKELETAL SYSTEM NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 25 1­4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL GLYCIDAMIDE Test Type: 90-DAY Route: DOSED WATER CAS Number: 5694-00-8 Specie.s /Strain: MICE/86C3F11NCTR · Pathologist: OLSON, G. 1 DAy ON TEST ,o 1 0 0 1 0 0 3 1 1 3 1 2 3 1 0 1 , 0 0 0 0 0 0 , 1 , 0 0 3 1 4 3 2 1 3 2 2 I Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07/26/04 Lab: NCTR I1.1 0 0 ............................................. ................ ................................. ...................................................................... C57BU6N XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 o0 o0 o0 MICE F~MALE 0.70 Glycid F ANIMAL :o a 3 2 3 I 3 2 4 *TOTALS ALIMENTARY SYSTEM Mesentery Fat, Necrosis 1 + 2 1 2.0 CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE I GENERAL BODY SYSTEM NONE GENITAL SYSTEM Ovary Cyst , + x 1 HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM NONE • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation BLANK .. Not examined microscopically I .. Insufficient tissue + .. Tissue examined microscopically Page 26 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TDMS No. 20314 - 02 P09: NON­NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90­DAY Route: DOSED WATER GLYCIDAMIDE CAS Number: 5694­00­8 Species/Strain: MICE/86C3F1 /NCTA Pathologist: OLSON, G. 1 DAy ON TEST I0 111 1 I 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 3 0 3 0 3 0 3 0 3 0 3 0 3 2 1 3 1 4 2 1 2 2 2 3 2 4 0 0 0 0 I0 1 l 1 0 0 0 Date Report Reqsted: 0612912006 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07/26/04 lab: NCTR . ..............................i........................,...................................................................................................... C57BU6N XC3HJHEN MTV­NCTR MICE FEMALE ANIMAL ID 0.70 Glycid F 0 3 1 1 11 *TOTALS MUSCULOSKELETALSYSTEM NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE • + x I .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade .. Tissue examined microscopically M .. Missing tissue .. Lesion present A .. Autolysis precludes evaluation .. Insufficient tissue BLANK .. Nol examined microscopically Page27 1­4 .. lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314- 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Gl.,YCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26104 / 07/26/04 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR DAY ON TEST 11111 1 0 0 0 I0 0 0 0 0 0 1 1 11 11 2 ' 0 3 0 0 0 0 0 .................................... ............... ....................................................................................................................... C57BU6N XC3HJHEN MTV-NCTR MICE FEMALE ANIMAL ID 1.41 Glycid F o0 0o o0 o0 o0 o0 o0 o0 2 7 1 2 7 2 2 7 3 2 2 7 4 8 1 2 8 2 2 8 3 2 8 4 • TOTALS ALIMENTARY SYSTEM Esophagus + 1 G allbladder A 0 Intestine Large, Cecum A 0 Intestine Large, Colon A 0 Intestine Large, Rectum A 0 Intestine Small, Duodenum A 0 Intestine Small, Ileum A 0 Intestine Small, Jejunum A 0 Liver + 1 Pancreas A 0 Salivary Glands + 1 Stomach, Forestomach A 0 • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autotysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 28 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 4) Marked 2) Mild TOMS No. 20314 · 02 Test Type: 90-0AY P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Reqsted: 06/29/2006 GLYCIDAMIDE Route: DOSED WATER CAS Number: 5694­00­8 Time Report Reqsted: 08:34:07 First Dose M/F: 07126104107126104 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab:NCTR DAY ON TEST 1 1 01 1 20 01 1 01 1 0 0 0 0 10 3 2 7 1 2 7 2 2 2 2 7 7 8 3 4 1 01 1 01 1 01 0 0 0 ............................................. ····1····· .... ·································································································· C57BU6N XC3H/HEN MTV-NCTR Ig g g g g g g g MICE FEMALE ANIMALID 1.41 Glycid F I l 2 8 2 2 8 3 2 8 4 *TOTALS Stomach, Glandular A Tongue + , Blood Vessel + 1 Heart + 0 CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM Adrenal Cortex + 1 Adrenal Medulla + 1 Islets, Pancreatic + 1 Parathyroid Gland + 1 Pituitary Gland M 0 Thyroid Gland A 0 GENERAL BODY SYSTEM • .. x l .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade .. T:s~!' examined microscopically M .. Missing tissue .. Lesion present A .. Autolysis precludes evaluation .. Insufficient tissue BLANK .. Not examined microscopically Page 29 1­4 .. Lesion qualified as: 1) Minimal 3) Moderate 4) Marked 2) Mild TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIDE Route: DOSED WATER CAS Number: 5694·00-8 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST C57BLJ6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 1.41 Glycid F 01 0 0 0 2 l I , I, 7 1 l Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07126104 107/26/04 Lab:NCTR 110 01 110 01 0 0 0 0 0 0 2 3 0 0 2 0 0 2 0 0 2 olo 72 717 3 4· 0 0 2 8 0 2 8 1 2 0 0 0 0 0 0 2 8 3 8 2 *TOTALS 4 NONE GENITAL SYSTEM Clitoral Gland + 1 Ovary + 1 Uterus + 1 Bone Marrow + 1 Lymph Node, Mandibular + 1 Lymph Node, Mesenteric M 0 Spleen A 0 Thymus A 0 M 0 HEMATOPOIETIC SYSTEM INTEGUMENTARY SYSTEM Mammary Gland • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. nssue examined microscopically Page 30 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Mark.ed TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL GLYCIDAMIDE Test Type: 90-DAY Dat.e Report Reqsted: 06/29/2006 Route: DOSED WATER CAS Number: 5694-00-8 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 I 07126104 Species/Strain: MICE186C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR , 0 DAy ON TEST , 0 , 0 , I o 0 2 I 0 10 10 10 C57BU6N XC3H/HEN ~· ANIMAL ID 1.41 Glycid F Skin , ., 0 3 1, 0 1 0 0 I0 . .g. ·i···gTg....g...g..i.. . · ·i MICE FEMALE , 0 2 2 2 2 2 2 2 2 7 7 7 7 8 8 8 8 1 2 3 4 ' 1 2 3 4 *TOTALS A 0 + 1 Brain, Brain Stem A 0 Brain, Cerebellum A 0 Brain, Cerebrum A 0 Lung + 1 Nose + 1 Trachea A 0 MUSCULOSKELETALSVSTEM Bone, Femur NERVOUS SYSTEM RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation BLANK .. Not examined microscopically I .. Insufficient tissue Page 31 + .. Tissue examined microscopically 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 4) Marked 2) Mild TOMS No. 20314- 02 Test Type: 90-DAY P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL GLYCIDAMIDE Route: DOSED WATER Species/Strain: MICE/86C3F1/NCTR DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 1.41 Glycid F ,I First Dose M/F: 07126104107/26/04 Pathologist: OLSON, G. Lab: NCTR , 3 0 0 0 0 0 0 0 2 2 7 18 8 2 8 3 1 1 0 10 0 0 0 0 0 0 0 0 0 0 0 2 7 2 2 7 3 0 2 0 0 2 7 1 CAS Number: 5694-00-8 1 0 0 1 4 0 2 1 Eye A Harderian Gland + 2 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 0 1 0 0 0 0 2 8 4 * TOTALS 0 URINARY SYSTEM Kidney + 1 Urinary Bladder A 0 * .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 32 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BV INDIVIDUAL ANIMAL Test Type: 90-DAY Date Report Reqsted: 06/29/2006 GLYCIDAMIDE Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07/26/04 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR DAy ON TEfT I 11 111111 I 1 I 1 I 1 1 0 0 0 C57BU6N XC3H/HEN ~ - ~· - · · · r ·~ 0 · -~· 0 0 0 .Q · ~· 0 0 ·~ I 0 0 0 0 0 0 r ~ ·~ · ~ · · - ·· · · · · ·· ·· ·· ·· ·· -~ - . ···. · · · · · ·· · · · 0 0 0 0 0 0 0 0 3 5 3 5 3 5 3 5 3 3 3 3 t 2 3 4 616 I6 6 1 2 3 4 Esophagus + + + + + + + + 8 Gallbladder + + + + + + + + 8 Intestine Large, Cecum + + + + + + + + 8 Intestine Large, Colon + + + + + + + + 8 Intestine Large, Rectum + + + + + + + + 8 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 Liver + + + + + + + + 8 Pancreas + + + + + + + + 8 Salivary Glands Infiltration Cellular, Lymphoid + + + + + + + + 8 Stomach, Forestomach + MICE FEMALE 3.52 Glycid F ANIMAL ID *TOTALS ALIMENTARY SYSTEM 1 1.0 1 + + + + + + 8 + • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severi1y grade M .. Missing tissue x .. Lesion present . A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 33 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 P09: NON-NEOPLASTIC L ESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIOE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose MJF: 07/26/04 / 07/26/04 Species/Strain: MICEJB6C3F1/NCTR Pathologist: OLSON, G. lab: NCTR 1 DAY ON TEST 1, 0 10 0 0 1 1 0 0 0 0 1 0 0 111 , , , 0 0 0 0 0 0 ···························.··· ····1························· ·································································································· C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE o o o o o o o o 0 0 0 0 0 0 0 0 3 5 3 515 3 3 5 3 6 3 6 3 6 1 2 3 I I 3 6 4 1 2 3 4 Stomach, Glandular + + + + + + + + 8 Tongue + + + + + + + + 8 Blood Vessel + + + + + + + + 8 Heart + + + + + + + + 8 + I + 1 + , + + + + 1 ANIMAL ID 3.52 Glycid F * TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM Adrenal Cortex Subcapsular, Hyperplasia 1 4 1.0 1 ·--························-··························--·-························································································-···································· Adrenal Medulla + I + + + + + 7 + ....................................................................................................................................................... ........... ..­ Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland + + + + + + + + 8 Pituitary Gland + + + + + + + + 8 Thyroid Gland + + + + + + + 8 ................................................................................................................................................................................. + • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page34 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL TOMS No. 20314 - 02 Test Type: 90-DAY GLYCIDAMIOE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00­8 First Dose MIF: 07126104 107/26/04 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR Ig 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 3 3 5 5 1 2 3 1 1 0 11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 3 3 3 3 5 5 6 6 6 6 4 1 2 3 4 Clitoral Gland ++M+­r++ + 7 Ovary + + + + + + + + 8 Uterus + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular + + + + + + + + 8 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + + + + 8 DAY ON TEST C57BLJ6N XC3H/HEN MTV­NCTR MICE FEMALE ANIMAL ID 3.52 Gl) cid F 1 1 * TOTALS GENERAL BODY SYSTEM NONE GENITAL SYSTEM HEMATOPOIETIC SYSTEM INTEGUMENTARY SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mear:i severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 35 1­4 .. Lesion qualifie­0 as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GlYCIDAMIDE Route: DOSED WATER CAS Number: 5694-00-8 Species/Strain: MICE/86C3F1 /NCTR . Pathologist: OLSON., G. DAy ON TEST ~6:!fHniEN ~:· I 1 0 0 11 11 0 0 0 0 r- T ·~ ANIMAL ID 0 I, I o,o 0 0 0 o,o 0 0 6 1 1 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 I 07126104 Lab: NCTR 1 ­1 TfTI T.... . . . . . .... . . . 5 1 5 2 5 3 5 4 6 1 6 2 I3 6 4 Mammary Gland + + + + + + + + 8 Skin + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain. Cerebrum + + + + + + + + 8 Peripheral Nerve Axon, Degeneration + + + + + + + 1 + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 3.52 Glycid F *TOTALS MUSCULOSKELETALSYSTEM NERVOUS SYSTEM 1 1.0 • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 36 1-4 .. lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20314 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL GLYCIOAMIDE Test Type: 90-DAY Route: DOSED WATER CAS Number: 5694-00-8 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAVON TEST 111 0 0 11 0 01 0 0 o, o 0 0 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07/26/04 Lab: NCTA 10 110 110 110 0 0 0 0 ······························ ····1·········· ··········r···· .................................................................................................. I 0 0 0 3 5 4 0 0 3 313 6 1 6 2 6 3 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 Eye + + + + + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 0 0 0 0 3 5 1 0 3 5 2 0 3 5 3 Lung + + Nose + Trachea C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 3.52 Glycid F 1 0 0 I 0 3 6 4 * TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM YRINARY SYSTEM *** END OF REPORT *** • + x I .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade .. Tissue examined microscopically M .. Missing tissue .. Lesion present A .. Autolysis precludes evaluation .. Insufficient tissue BLANK .. Not examined microscopically Page 37 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked P09: NO~EPLASTIC TOMS No. 20313 - 04 LESIONS BY INDIVIDUAL ANIMAL ACRYLAMIDE Test Type: 90-0AY CAS Number: Route: DOSED FEED 79-0~ 1 Pathologist: OLSON, G. Species/Strain: MICE/86C3F1/NCTR C Number: C20313B Lock Date: Not Entered. Cage Rang~: ALL Date Range: ALL Reasons For Removal: ALL Removal Date Range: ALL Treatment Groups: Include ALL Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02104 / 08102104 lab: NCTR TOMS No. 2031 3 - 04 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type: 90-DAY ACRYLAMIDE Roule: DOSED FEED CAS Number: 79­06­1 Species/Strain: MICE/B6C3F1/NCTR C57BLJ6N XC3HIHEN MTV-NCTR MICE FEMALE Hyperplasia, Lymphoid Spleen Hyperplasia, Lymphoid Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:06:38 First Dose M/F: 08/02104 / 08102104 Pathologist: OLSON, G. lab: NCTR Control Feed F 18.5 PPM Acryl F 37 PPM Acryl F 74 PPM Aery! F 185 PPM Acryl F (8) 5 (63%) (0) (0) (0) (0) (8) (0) (0) (0) (8) (8) (0) (0) (0) (8) 1 (13%) (0) (0) {O) (0) INTEGUMENTARY SYSTEM None MUSCULOSKELETAL SYSTEM Skeletal Muscle Atrophy NERVOUS SYSTEM Peripheral Nerve Axon, Degeneration -RESPIRATORY SYSTEM None SPECIAL SENSES SYSTEM None URINARY SYSTEM Urinary Bladder Lumen, Dilatation (8) .. a - Number of animals examined microscopically at site and number of animals with lesion Page7 TOMS No. 20313 - 04 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE{a) Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79­06· 1 Pathologist: OLSON, G. Species/S1rain: MICE/86C3F1/NCTR C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE 370 PPM Acryl F Disposition Summary Animals Initially in Study Early Deaths Survivors Terminal Sacrifice Animals Examined Microscop ically 8 8 8 ALIMENTARY SYSTEM None CARDIOVASCULAR SYSTEM None ENDOCRINE SYSTEM Adrenal Cortex Accessory Adrenal Cortical Nodule Subcapsular, Hyperplasia (8) 1 (13%) 2 (25%) GENERAL BODY SYSTEM None GENITAL SYSTEM Ovary Anestrus Cyst (8) 8 (100%) 1 {13%) HEMATOPOIETIC SYSTEM Lymph Node, Mandibular Hyperplasia, Lymphoid Lymph Node, Mesenteric (8) 1 (13°/,.) (8) a - Number of animals examined microscopically at site and number of animals with lesion Pages Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:06:38 First Dose M/F: 08/02/04 / 08/02/04 Lab: NCTR TOMS No. 20313 - 04 P03: INCIDENCE RATES OF NON-NEOPLASTIC LESIONS BY ANATOMIC SITE(a) Test Type : 90·DAY ACRYLAMIDE CAS Number: 79·06-1 Route: DOSED FEED Species/Strain: MICE/B6C3F1/NCTA C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE Hyperplasia, Lymphoid Spleen Hyperplasia, Lymphoid Pathologist: OLSON, G. 370 PPM Acryl F 1 (13%) (8) 1 (13%) INTEGUMENTARY SYSTEM None MUSCULOSKELETALSYSTEM Skelelat Muscle Atrophy (8) 7(88%) NERVOUS SYSTEM Peripheral Nerve Axon, Degeneration (7) 7 (100%) RESP IRATORY SYSTEM None SPECIAL SENSES SYSTEM None URINARY SYSTEM Urinary Bladder Lumen, Dilatation (8) 7 (88%) *** END OF REPORT *** a · Number of animals examined microscopically at site and number of animals with lesion Page 9 Date Report Reqsted: 07131 /2006 Time Report Reqsted: 12:06:38 First Dose MJF: 08/02104 / 08/02/04 Lab: NCTR Pathology Report Appendix Ill - Neoplasms by Individual Animal (Pathology Report 4) A-3 ~MS ~0 . 2'1313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90·DAY ACRYLAM IDE Route: DOSED WATER CAS Number: 79-06· 1 Species/Strain: MICE/86C3F1 /NCTR Pathologist: OLSON, G. C Number: C20313 Lock Date: Not Entered. Cage Range: ALL Date Range: ALL Reasons For Removal : ALL Removal Date Range: ALL Treatment Groups: Include ALL Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04/07/12/04 Lab:NCTR TOMS No. 20313­ 02 Test Type: 90-DAY Route: DOSED WATER P04: NEOPLASMS BY INDIVIDUAL ANIMAL ACRYLAMIDE CAS Number: 79­06­1 Pathologist: OLSON, G. Species/Strain: MICE/86C3F1/NCTR DAy ON TEST C57BlJ6N XC3H/HEN ~; ~ ~; I I 0 0 10 0 9 9 9 9 9 19 ..........~ .. f -~ .. ·-~ 0 0 09 9 9 · · ·~ 9 0 10 10 9 9 9 9 9 9 ..~· -~· Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/1 2104 / 07/12104 Lab: NCTR ·~ · 0 0 0 0 0 0 ................................................................... o o o o o o o o MICE MALE s s I 5 s 6 6 6 6 3 4 1 2 3 4 + + + + + + + 8 + + + + + M + + 7 Intestine Large, Cecum + + + + + + + + 8 Intestine Large, Colon + + + + + + + + 8 Intestine Large, Rectum + + + + + + + + 8 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 liver + + + + + + + + 8 Pancreas + + + + + + + + 8 Salivary Glands + + + + + + + + 8 Stomach, Forestomach + + + + + + + + 8 ANIMAL ID 1 I2 Esophagus + Gallbladder Control Water M *TOTALS ALIMENTARY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page2 TOMS No. 20313- 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE CAS Number: 79-06-1 Route: DOSED WATER Species/Slrain: MICE/86C3F 1/NCTR Pathologist : OLSON, G. DAy ON Tt=ST C57BU6N XC3H/HEN ~; Io l o First Dose M/F: 07/12104 / 07/12104 Lab: NCTA olo o o o o 9 9 9 , 9 · · · i ·~ · ·~ Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 ·~ 9 9 9 9 0 0 9 9 9 9 9 9 9 9 · -~ · 1 ·~ · 1 · ~· · ~ · 0 0 0 0 15 5 5 5 6 1 2 3 4 1 6 16 6 2 3 4 + + + + + + + + 8 + + + + + + + + 8 Blood Vessel + + + + + + + + 8 Heart + + + + + + + + 8 Adrenal Cortex + + + + + + + + 8 Adrenal Medulla + + + + + + + + 8 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland + + + + + + + + 8 Pituitary Gland M + + + + + + + 7 Thyroid Gland + + + + + + + 8 Control Water M 0 0 o o o o o o o o MICE MALE ANIMAL ID Stomach, Glandular Tongue I *TOTALS ...........................................................................................................................................................................................................:....... CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM + GENERAL BODY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 3 TOMS No. 20313- 02 Test Type: 90-0AY Route: DOSED WATER P04: NEOPLASMS BY INDIVIDUAL ANIMAL ACRYLAMIDE CAS Number: 79-06-1 · Pathologi st: OLSON, G. Species/Strain: MICE./86C3F1/NCTR DAY ON TEST 0 10 19 9 9 C57BU6N XC3H/HEN ~;· r 9 ~· 0 9 0 9 0 9 0 9 0 10 9 9 9 9 9 9 9 ·~ 0 0 5 1 Epididymis · : ~· ·~ ·~ 0 0 0 0 5 5 5 6 2 3 4 1 + + + + Preputial Gland + + + Pros1ate + + Seminal Vesicle + Testes Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07/12/04 Lab: NCTR 9 ·~ ·· ·· · · · · · · · ·· · ·· · · ·· ·· · · 0 0 6 6 6 2 3 4 + + + + 8 + + + + + 8 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 ++MM++++ 6 o o o o o o o o MICE MALE ANIMAL ID Control Water M *TOTALS NONE GENITAL SYSTEM HEMATOPOIETIC SYSTEM Bone Marrow Lyrnp~ · Node, Mandibular Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + + + + 8 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page4 TOMS No. 20313 - 02. P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. DAVON TEST C57BU6N XC3H/HEN ~; rn 9 9 o o 0 0 o o o o o o 0 : 1 : 1 0 ANIMAL ID I I 5 1 0 5 2 0 5 3 Lab: NCTR I9 9 0 0 0 0 5 4 0 6 3 0 6 4 6 1 First Dose M/F: 07/12/04 / 07/12/04 o o o. 9 9 · · · 1 · ·~ · r ·~ ·~ · ·~ · MICE MALE Control Water M 1 0 9 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 6 2 *TOTALS INTEGUMENTARY SYSTEM Mammary Gland M M M + M + M M Skin + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 2 MUSCULOSKELETALSYSTEM NERVOUS SYSTEM M .. Missing tissue A. .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. lnsutticient tissue Page 5 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON. G. 90 1 90 9 9 90 1 09 1 90 9 9 9 0 0 0 0 o o o o 5 1 5 2 I 00 o .o 515 3 + + Lung + Nose Trachea DAY ON TEST Date Report Reqsted: 06/2912006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 I 07112104 Lab: NCTR 90 1 09 1 90 9 9 9 ........................... .. ........ ........................ ..... ........... . .............. ....................................................................... ....... 0 0 0 0 00 0 0 0 0 4 6 1 6 2. 6 3 6 4 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 + + + +· + + + + 8 Eye + + + + + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 C57BU..6 N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID Control Water M Spinal <;;ord, Thoracic o o * TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM URINARY SYSTEM SYSTEMIC LESIONS • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically · .. Lesion present .. Insufficient tissue Page6 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 02 Test Type: 90·DAY ACAYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE186C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST I 0 9 I III I I 0 9 0 9 0 9 0 9 0 9 9 9 9 9 9 19 0 0 0 0 0 0 0 0 0 0 1 2 3 4 1 + + + + + 9 0 9 Dat e Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 I 07112104 Lab: NCTA I 0 9 9 ......................................................·······1·······.··············........·········...·······..................... C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID Control Water M Multiple Organ 0 0 I I 0 0 0 0 2 3 4 + + + o o o o o o o o 5 5 s 5 6 6 6 6 I *TOTALS 8 M .. Missing tissue A •. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page7 TOMS No. 20313-02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Date Report Reqsted: 06/29/2006 ACAYLAMIDE Time Report Reqsted: 08:1 5:54 First Dose M/F: 07/1 2/04 / 07/12/04 CAS Number: 79­06­1 Route: DOSED WATER Spedes/Strain: MICE/86C3F1/NCTA Lab: NCTA Pathologist: OLSON, G. DAy ON TEST Io~ Io~ Io~ ~ Io I o~ ~ Io l o~ Io~ ...................................... C57BlJ6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 0.35 Acryl M 0 0 1 3 1 0 0 1 3 2 . .......... . ····································································· 0 0 1 3 3 0 0 0 1 0 1 3 4 1 4 0 0 1 4 0 2 3 + + 0 1 4 I 0 0 1 4 * TOTALS 4 ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM Preputial Gland 2 HEMATOPOIETJC SYSTEM NONE INTEGUMENTARY SYSTEM NONE MUSCULOSKELETALSYSTEM M .. Missing tissue A .. Autolysis prec.ludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 8 TOMS No. 20313 • 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL ACAYLAMIDE Test Type: 90-DAY Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST C57Bll6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 0.35 Acryl M Date Report Reqsted: 06!29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04/07/ 12/04 Lab: NCTR o o olo o olo o 9 9 9 9 9 9 9 9 19 , 9 9 9 9 0 0 0 0 0 0 0 0 0 10 0 0 1 1 313 1 2 9 0 0 9 19 0 0 1 1 1 1 1 1 3 3 4 4 4 4 3 4 1 2 3 4 + + *TOTALS NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE SYSTEMIC LESIONS Multiple Organ 2 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 9 TDMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test T;·;;>e: QO-DAY ACAYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1JNCTR Pathologist: OLSON, G. DAy ON TEST I 0 1 0 0 10 9 9 9 0 0 0 10 9 9 9 9 9 I9 9 9 9 9 j 9 0 0 0 8 3 8 4 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12104 / 07/12104 Lab: NCTR 9 ...........................···r.............................·1·....•.............••••..•.•...••......••....••......•.....•.....••••............•.........•...•.. II C57Bu6N Xe3H/HEN MTV-NCTR 0 0 0 0 0 0 0 0 MICE MALE 1 1 1 1 1 1 2 3 4 ANIMAL ID 0.70 Acryl M 0 7 7 17 7 0 8 I 0 1 I8 I1 I2 0 1 0 1 * TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM NONE HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM NONE MUSCULOSKELETALSYSTEM M .. Missing tissue A .. Aulolysis precludes evaluation BLANK .. Not examined microscopically * .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 10 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY f\CRYLAMIDE Route: DOSED WATER CAS Number: 79­06­1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST I~ 09 1 9 0 19 0 1 09 Time Report Reqst ed: 08:15:54 First Dose M/F: 07112104 / 07/12104 lab: NCTR 90 1 90 1 09 9 9. 9 9 9 9 9 0 0 1 7 0 0 0 1 0 0 1 0 0 8 0 0 1 8 2 3 0 0 1 7 4 1 + + + + + + + + + + 9 Dat e Report Reqsted: 06/29/2006 .................................. .......... .............. ················································································· C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 0.70 Acryl M Skeletal Muscle lg 1 I~ 0 1 7 1 2 8 3 8 4 + + + + 8 + + + + + 8 + + + + + 8 *TOTALS NERVOUS SYSTEM Peripheral Nerve RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE SYSTEMIC LESIONS Multiple Organ • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Nol examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion pfesent .. Insufficient tissue Page 11 TOMS No. 20313- 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACAYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICEJB6C3F1/NCTR Pathologist: OLSON, G. DAy ON TEST C57BU6N XC3HIHEN ~:;· ···· · l I I I I I ANIMAL ID 1.41 Acryl M. First Dose M/F: 07/12104 / 07/12104 lab: NCTR o9 o9 o9 o9 o9 o9 o9 o9 9 i 9 19 1 -~· 9 9 9 h 1 9 9 1 9 2 3 4 0 1 + + + + + 9 9 T ~·ir o o o o MICE MALE Date Report Reqsted: 06/29/2006 nme Report Reqsted : 08:15:54 1 9 i....... .. ..... I0 1 1 0 2 , 3 0 4 + + + " TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM Testes 8 HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM NONE MUSCULOSKELETALSYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK -· Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 12 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 02 Test Type: 90-DAY ACAYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Pathologist: OLSON, G. Species/Strain: MICE/86G3F1/NCTR DAy ON TEST Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07112/04 / 07/12/04 Lab: NCTR I ololol 9 9 I 9 olololol 9 9 9 9 o 9 9 9 9 9 9 9 9 9 ................................. ....................................................................................................................................... C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID o0 o0 o0 o0 o0 o0 o0 o0 oooo 1 1 1 1 9 1 9 2 9 3 9 4 0 1 0 2 0 3 0 4 + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 1.41 Acryl M Skeletal Muscle * TOTALS NERVOUS SYSTEM RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINAnY SYSTEM NONE SYSTEMIC LESIONS M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 13 P04: NEOPLASMS BY INDIVIDUAL ANIMAL ACRYLAMIDE TDMS No. 20313 - 02 Test Type: 90-0AY Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR . Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE MALE 1.4~ ANIMAL ID A!:ryl M Multiple Organ • + x I I ~9 0 0 0 9 9 9 9 9 9 9 0 0 0 0 0 0 r ~· - ~· 0 0 0 0 9 19 9 9 !il 0 0 0 0 .......... ·········· ..... 0 0 0 0 1 1 1 1 9 10 4 1 0 3 0 2 0 2 + + + + + + 9 + + First Dose M/F: 07/12104 / 07/12104 Lab:NCTR 9 9 3 9 1 Time Report Reqsted: 08:15:54 oI o to 0 9 Date Report Reqsted: 06/29/2006 * TOTALS 4 8 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present .. Insufficient tissue Page 14 TOMS No. 20313 • 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79--06-1 Species/Strain: MICE/B6G3F1 /NCTR Pathologist: OLSON, G. 09 109 DAY ON TEST 9 9 C57BLJ6N XC3H/HEN ~;· ·~ 0 9 9 ..~. -~ 0 9 9 r 0 0 0 1 1 1 2 1 3 Esophagus + + Gallbladder + Intestine large, Cecum ~· 0 9 9 010 9 9 9 -~· . ... 9 ~ -~. Date Report Reqst ed: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07/12/04 Lab: NCTR 0 9 9 ·~ · · . ... .. .. . .. . ... . . .... . . ... .. . ....... . ... . . . .... . . . . . . . 0 0 0 0 0 1 4 2 1 2 2 2 3 2 4 + + + + + + 8 + + + + + + M 7 + + + + + + + + 8. Intestine Large, Colon + + + + + + + + 8 Intestine large, Rectum + + + + + + + + 8 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 liver + + + + + + + + 8 Pancreas + + + + + + + + 8 Salivary Glands + + + + + + + + 8 Stomach, Forestomach + + + + + + + + 8 o o o o o o o o MICE MALE ANIMAL ID 3.52 Acryl M I * TOTALS ALIMENTARY SYSTEM • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscoplcally; Total animals with tumor .. Tissue examined microscopically .. lesion present .. Insufficient tissue Page 15 TOMS No. 20313 • 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIOE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTA Pathologist: OLSON, G. I IIIIIl First Dose M/F: 07/12104 / 07/12104 l ab: NCTR 0 9 0 9 0 9 0 9 0 9 0 9 0 9 9 9 9 9 9 9 9 0 0 0 0 0 0 I 00 o o I o o o o o o 1 2 1 3 4 2 1 2 2 2 3 Stomach, Glandular + + + + + + + + 8 Tongue + + + + + + + + 8 Blood Vessel + + + + + + + + 8 Heart + + + + + + + + 8 Adrenal Cortex + + + + + + + + 8 Adrenal Medulla + + + + + + + + 8 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland + + M + + + + + 7 Pituitary Gland +++M++++ 7 Thyroid Gland + DAY ON TEST 0 9 Date Report Reqsted: 06/29/2006 Time Report Reqst ed: 08:1 5:54 9 ······························ .............. ···············r ····················································· ............................................ C57BU6N XC3H/HEN. MTV-NCTR MICE MALE 3.52 Acryl M ANIMAL ID 0 0 11 1 0 0 0 0 11 I I 0 0 2 4 *TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM + + + + + + 8 + GENERAL BODY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 16 TDMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: Route: DOSED WATER CAS Number: 79-06-1 90-DAY ACRYLAMIDE Species/Strain: MICEJB6C3F1 /NCTR Pathologist: OLSON, G. Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose MJF: 07/1 2/04 / 07/12/04 Lab: NCTR DAYON TEST I 010101010101010 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 9 .............................·r.............................. .................................................................................................. C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID o0 o0 o0 o0 o0 o0 o0 o0 o o o o o o o o 1 1 I2 1 3 1 4 2 1 2 2 2 3 2 4 Epididymis + + + + + + + + 8 Prepulial Gland + + + + + + + + 8 Prostate + + + + + + + + 8 Seminal Vesicle + + + + + + + + 8 Testes + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular M+++++++ 7 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + +' + + 8 3..52 Acryl M 1 * TOTALS NON E GENITAL SYSTEM HEMATOPOIETIC SYSTEM • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion presenl I .. Insufficient tissue M .. Missing tissue A .. Autolysls precludes evaluation BLANK .. Not examined microscopically Page 17 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICEJ86C3F1/NCTR Pathologist: OLSON, G. DAy ON TEST I 0 9 9 I I II 0 9 9 0 9 9 0 I 0 9 9 9 j 9 0 9 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12104 / 07/12/04 Lab: NCTR II 0 9 9 0 9 9 0 0 ························r ···· .................. 1..........1.................................................................................................. 0 0 0 0 0 1 1 1 2 1 3 Mammary Gland M M M M Skin + + + e0!1e Femur + + Skeletal Muscle + Brain, Brain Stem C57BU6N XC3H/HEN MTV-NCTR MICE MALE 0 0 0 0 I 0 0 2 2 2 2 3 2 4 M M M M 0 + + + + + 8 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 ANIMAL ID 3.52 Acryl M 0 0 0 o o o o o o o o 1 I4 I1 • TOTALS INTEGUMENTARY SYSTEM MUSCULOSKELETAL SYSTEM NERVOUS SYSTEM • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present .. Insufficient tissue Page 18 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: OOSF.D WATER ACRYLAMIDE ·cAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. 0 DAY ON TEST C57BU6N XC3H/HEN ~;· 9 9 0 9 9 · · ·-~ 0 9 9 0 9 9 ·~ 9 -·~ 0 0 1 1 0 0 1 2 0 0 1 3 + + Lung + Nose Trachea I I olo r ·~ -~· o9 o9 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12104 / 07/12104 Lab: NCTR 9 19 9 9 ·· · · ·· · · ·· ·· 0 0 1 4 0 0 2 1 0 0 2 2 0 0 2 3 0 0 2 4 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 Eye + + + + + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 MICE MALE ANIMAL ID 3.52 Acryl M Spinal Cord, Thoracic *TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM URINARY SYSTEM SYSTEMIC LESIONS M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 19 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: Species/Strain: MICE/86C3F1 /NCTR Pathologist: OLSON, G. DAY ON TEST ~6c 3 HJEN ~;:· 79-0~1 lo j o 9 9 · · · ; ·f ANIMAL ID 3.52 Acryl M Multiple Organ 9 9 Date Report Reqsted: 06/29/2006 nme Report Reqsted: 08:15:54 First Dose M/F: 07/ 12/04 / 07112/04 Lab: NCTR o o o o o o 9 9 -~ T f 9 9 9 9 9 9 9 9 9 9 2 2 3 2 4 + + + .f .f]""gTf .g····························································································· 1 1 1 2 1 3 1 4 2 1 + + + + + I2 . •TOTALS 8 *** END OF MALE DATA *** M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 20 TOMS No. 20313- 02 Test Type: 90-DAY Route: DOSED WATER P04: NEOPLASMS BY INDIVIDUAL ANIMAL ACRYLAMIDE CAS Number: 79­06­1 Pathologist: OLSON, G. Species/Strain: MICEJB6C3F1/NCTR DAY ON TEST 1 0 0 110 1 0 1 0 1 1 0 0 1 0 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 Fi rst Dose M/F: 07/12104 / 07/12/04 Lab: NCTR 1 0 0 0 O· 0 0 0 I 00 0 0 0 0 0 0 0 0 0 0 0 0 7 7 7 8 8 18 2 3 7 4 8 1 1 2 3 4 Esophagus + + + + + + + + 8 Gallbladder + + + + + + + + 8 Intestine Large, Cecum + + + + + + + + 8 Intestine Large, Colon + + + + + + + + 8 Intestine Large, Rectum + + + + + + + + 8 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 Liver + + + + + + + + 8 Pancreas + + + + + + + + 8 Salivary Glands + + + + + + + + 8 Stomach, Forestomach + + + + + + + + 8 0 ··································· ·········· .... ····· ····r ······························································· C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE Control Water F ANIMAL ID I 0 0 o o o o o o o o * TOTALS ALIMENTARY SYSTEM M .. Missing tissue A •. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. nssue examined microscopically x .. Lesion present I .. Insufficient tissue Page 21 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYL.AMIDE Route: DOSED WATER CAS Number: 79-06­1 Species/Str ain: MICEJ86C3F1/NCTR First Dose M/F: 07/12104 / 07112104 Lab: NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN ~; ~ ~ ; ~· · l 1 ·~ 1 1 1 , I, 1 1 1 o o o olo o o o 0 o1 , o 0 0 0 0. 0 ·~ r ·~ ·~ Date Report Reqsted: 06/29/2006 Time Report Reqsted : 08:1 5:54 · ·~ · · · · ·· . · 0 0 0 0 0 0 0 0 17 1 7 2 7 3 7 4 8 1 8 2 8 3 8 4 Stomach, Glandular + + + + + + + + 8 Tongue + + + + + + + + 8 Blood Vessel + + + + + + + + 8 Heart + + + + + + + + 8 Adrenal Cortex + + + + + + + + 8 Adrenal Medulla + + + + + + + + 8 Islets, Pancreatic + + + + + + + 8 Parathyroid Gland +++M++++ 7 Pituitary Gland + + + + + + + + 8 Thyroid Gland + + + + + + + + 8 o o o o o o o o MICE FEMALE Control Water F ANIMAL ID * TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM + GENERAL BODY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. nssue examined microscopically x .. Lesion present I .. Insufficient tissue Page22 TOMS No. 20313 ­02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Dat e Report Reqsted: 06/29/2006 ACRYLAMIDE CAS Number: 79--06-1 Time Report Reqsted: 08:1 5:54 First Dose M/F: 07/ 12/04 / 07/12/04 Test Type: 90-DAY Route: DOSED WATER Species/Strain: MICE/B6C3Fl/NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN ~;: MICE FEMALE ~ ~ ;~·. .. ANIMAL ID Control Water F l 1 0 0 1 0 0 1 0 0 0 1 0 l 0 0 0 7 1 0 7 2 0 7 3 0 7 4 0 8 1 Lab: NCTR 1 110 11 0 0 0 h... ···g ..i ··g· ..i. ·g­rg g···.... ................ I 0 8 2 0 0 0 8 3 0 8 4 I * TOTALS NONE GENITAL SYSTEM Clitoral Gland +++M++++ Ovary + + + + + + + + 8 Uterus + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular + + + + + + + + 8 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + . + + + + 8 Thymus + + + + + + + + 8 + + + + + + + + 8 7 HEMATOPOIETIC SYSTEM INTEGUMENTARY SYSTEM Mammary Gland M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x __ Lesion present I .. Insufficient tissue Page23 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-0AY ACRYLAMIDE Route: DOSED WATER CAS Number: 79­06·1 Species/Strain: MICEJ86C3F1/NCTR . Pathologist: OLSON, G. DAY ON TEST 1 1 , , , 0 0 0 0 0 0 0 0 0 0 01 1· 0, 0 0 0 10 0 0 0 0 0 0 0 0 0 0 0 o7 7o o7 0 0 0 0 8 2 8 3 8 4 0 C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 0 0 0 7 I 0 , 110 Date Report Reqsted : 06/29/2006 Time Report Reqsted: 08:1 5:54 First Dose M/F: 07/ 12/04 I 07112104 lab: NCTR 2 3 4 8 1 + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 Control Water F Skin I1 *TOTALS MUSCULOSKELETALSYSTEM NERVOUS SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present l .. Insufficient tissue Page24 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Date Report Reqsted: 06/29/2006 ACRYLAMIDE Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07/12/04 Test Type: 90-DAY Route: DOSED WATER CAS Number: 79­06­1 Species/Strain: MICE/B6C3Fl/NCTA Pathologist: OLSON, G. DAY ON TEST Lab: NCTR 01 1 01 1 10 1 01 1 01 1 01 1 01 1 01 0 0 0 0 0 0 0 0 ........ ...... ~ ...... ........................... . 0 0 0 7 0 0 0 7 0 0 0 7 0 0 0 7 0 0 0 8 0 0 0 8 0 0 0 8 0 0 0 8 1 2 3 4 1 2 3 4 Lung + + + + + + + + 8 Nose + + + + + + + + 8 Trachea + + + + + + + + 8 Eye + + + + + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 + + + + + + + + 8 C57BLJ6N XC3HJHEN MTV-NCTR MICE FEMALE ANIMAL ID Control Water F " TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM URINARY SYSTEM SYSTEMIC LESIONS Multiple Organ M .. Missing tissue A .• Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 25 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-0AY ACRYLAMIOE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICEJB6C3F1/NCTR Pathologist: OLSON, G. DAy ON TEST I Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07/12/04 Lab: NCTR 111111111111111 0 I0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 1 1 5 1 5 2 5 3 5 4 6 1 6 2 6 3 0 ··································· ................... ····r ····························· C57BU6N XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 o0 o0 o0 MICE FEMALE 0.35 Acryl F ANIMALIO I1 6 4 *TOTALS ALIMENTARY SYSTEM Liver + 1 + 1 CARDIOVASCULAR SYSTEM NONE ENDOCruNESYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM Ovary HEMATOPOIETIC SYSTEM NONE INTEGUMENTARYSYSTEM NONE M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopicalty; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 26 TOMS No. 20313- 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR ~· 0 r ~· 0 1 MICE FEMALE ANIMAL ID Lab: NCTA 1o 1111111111 o o o o o I 111 o o 0 0.35 Acryl F First Dose M/F: 07/ 12/04 / 07/12/04 Pathologist: OLSON, G. DAY ON TEST C57Bl16N XC3H/HEN Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 ACRYLAMIDE 5 1 0 ·~ 0 I 0 0 - ~· 0 1 1 5 2 5 3 0 0 0 0 · · ~· r ~· 0 0 0 1 1 6 3 6 4 1 1 1 5 4 6 1 6 2 0 · · ~· · · ·· · · ·· · · · · ··· · · - · · ·· ·- · · · ·- · · · • TOTALS MUSCULOSKELETALSYSTEM NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NCNC.: URINARY SYSTEM NONE SYSTEMIC LESIONS Multiple Organ 1 + M .. Missing tissue A .. AUlolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 27 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED WATER ACRYlAMIDE CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1 /NCTA Pathologist: OLSON, G. Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 I 07112104 Lab: NCTR I 111111111 0 0 0 0 111 0 0 11 0 0 0 0 0 0 0 0 ............................................[...............1··········..···················-·····............................................................. DAy ON TEST I 0 0 C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 0.70 Acryl F o0 o0 o0 o0 o0 o0 o0 o0 1 1 1 1 2 2 9 1 9 2 9 3 9 4 0 1 0 2 Ia I 2 2 0 0 4 *TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM NONE HEMATOPOIETIC SYSTEM Spleen 1 + INTEGUMENTARY SYSTEM NONE MUSCULOSKELETALSYSTEM M .. Missing tissue A .. Autolysis preoludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 28 TDMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Rout.e: DOSED WATER Time Repo rt Reqsted: 08:15:54 ·cAS Number: 79-06-1 Species/Strain: MICE/86C3F1 /NCTR 1 ~; ~ ;~· 0 0 ·· MICE FEMALE 0.70 Acryl F First Dose M/F: 07/12/04 / 07/12/04 . Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN Date Report Reqsted: 06/29/2006 ACRYLAMIDE ANIMAL ID Skeletal Muscle 10 110 1 , , 01 1 01 1 01 o oIo o o · 1 · ·~ · · -~ · ·~- · ~· -~· Lab: NCTR 0 0 0 0 -~ · · · ~· · ·· ·· ··· · · ·· · ·· ·· · . . . . ... . . . ... . .... .. . 0 0 9 3 0 1 9 4 2 0 1 2 0 2 0 2 0 3 0 2 0 4 + + + + + + + 8 + + + + + + + 8 + + + + 8 0 1 19 1 0 0 1 1 9 2 + + *TOTALS NERVOUS SYSTEM Peripheral Nerve RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM Eye + URINARY SYSTEM NO~ SYSTEMIC LESIONS Multiple Organ + + + + M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 29 TOMS No. 20313 • 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL ACRYLAMIDE CAS Number: 79-06-1 Test Type: 90-DAY Route: DOSED WATER Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAy ON TEST C57BU6N XC3H/HEN ~ ~ ~· · ·· · MICE FEMALE 1.41 Acryl F Time Report Reqsted: 08:15:54 First Dose MIF: 07/12104 I 07112104 Lab: NCTR Ig111111111111111 g g g g g gI g ·g- · r ·g · 1 ANIMAL ID Date Report Reqsted: 06/29/2006 1 1 1 ·i···g·i·g·r·g·r·g··i······························· ······························································· 1 1 1 1 2 1 3 1 4 + + + l 1 1 1 1 2 12 1 2 2 3 2 4 + + + *TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM Ovary + + 8 HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM Skin 1 + • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present .. Insufficient tissue Page 30 TOMS No. 20313 · 02 ~est P04: NEOPLASMS BY INDIVIDUAL ANIMAL Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: Species/Strain: MICE./86C3F1/NCTR 79­­06­1 Pathologist: OLSON, G. DAy ON TEST C57BU6N XC3H/HEN ~;· I,,, 0 · 0 ·~ ' 0 0 -~· I 11111 1 11 0 0 0 0 0 0 .. 0 0 0 0 1 0 0 0 0 ·~ · ·1 ·~ · · ~ · · · ~· · ·~ 0 0 1 1 1 1 1 2 + Peripheral Nerve ··· 1 1 1 1 1 3 4 2 1 2 2. 2 3 2 4 + + + + + + + 8 + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 1.41 Acryl F 1 First Dose MJF: 07/12/04 I 07112104 lab: NCTR 0 ANIMAL ID 0 Time Report Reqsted: 08:1 5:54 0 MICE FEMALE 0 Date Report Reqsted: 06/29/2006 111 *TOTALS MUSCULOSKELETALSYSTEM Skeletal Muscle NERVOUS SYSTEM RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE UR!NARY SYSTEM NONE SYSTEMIC LESIONS M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopical!y; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 31 TOMS No. 20313- 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79­06­1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST I 01 1 01 1 01 1 01 1 01 1 01 1 01 0 0 0 0 0 0 0 ................................... C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE 1.41 Acryl F ANIMAL ID Multirle Organ First Dose M/F: 07/12104 / 07/12104 Lab: NCTR 10 ,................................................................................................ 0 0 0 0 0 0 0 0 1 1 1 1 I 0 0 0 0 0 0 0 0 0 1 11 I 1 11 I 2 1 2 I3 4 1 1 1 1 2 2 2 2 3 4 + + + + + Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 + + 1 + *TOTALS 8 M .. Missing tissue A .. Autolysis precludes evaluation BLANK •. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 32 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 02 Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79'-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. 10 110 110 110 1 1 1 1 0 0 0 0 0 0 0 0 0 4 0 0 0 O, O 0 0 0 0 0 0 0 0 0 0 3 1 3 2 Esophagus + + Gallbladder ++ Intestine Large, Cecum + Intestine Large, Colon DAY ON TEST Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07/ 12/04 lab: NCTR 0 0 0 0 0 3 0 3 0 4 0 0 0 4 3 4 1 2 3 0 0 4 4 + + + + + + 8 ++ M+++ 7 + + + + + + + 8 + + + + + + + + 8 Intestine Large, Rectum + + + + + + + + 8 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 Liver + + + + + + + + 8 Pancreas + + + + + + + + 8 Salivary Glands + + + + + + + + 8 Stomach, Forestomach + + + + + + + + 8 C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 3.52 Acryl F * TOTALS ALIMENTARY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscor.ically x .. Lesion present I .. Insufficient tissue Page 33 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR First Dose MJF: 07/12/04 / 07/12/04 Lab: NCTR · Pathologist: OLSON, G. DAY ON TEST I 0 110 1100 1100 110 1100 1100 1· 00, 01 0 0 ··························-··· ­··· .......... ,......-············-···········-······························· 0 0 0 0 °0 3 4 4 1 4 2 4 3 4 4 + + + + + + 8 + + + + + + 8 Blooct Vessel +M++++++ 7 Heart + + + + + + + + 8 Adrenal Cortex + + + + + + + + 8 Adrenal Medulla + + + + + + + + 8 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland + +M+++++ 7 Pituitary Gland M+++++++ 7 Thyroid Gland + 7 0 0 0 0 0 0 0 3 1 3 2 3 3 Stomach, Glandular + + Tongue + + C57BLJ6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 3.52 AcryJ F _ 0 I• 0 0 o o o o o o o o *TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM + M +' + + + + GENERAL BODY SYSTEM M .. Missing tissue A .. Aut olysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 34 TOMS No. 20313 • 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-0AY ACRYLAMIDE CAS Number: 79­06­1 Route: DOSED WATER Species/St rain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST C578U6N XC3H/HEN MTV-NCTR MICE FEMALE I 1 01 110 0 0 01 0 0 0 0 0 0 0 0 10 10 0 0 0 0 0 0 11 0 0 0 0 01 1 01 0 0 0 0 0 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose MIF: 07/12/04 / 07/12/04 lab: NCTR 1 0 0 0 0 0 0 0 0 4 4 3 4 3 1 3131314 4 2 3 4 1 2 Clitoral Gland + + + + + + + + 8 Ovary + + + + + + + + 8 Uterus + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular M+++++++ 7 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + + + + 8 + + + + + + + + 8 ANIMAL ID 3.52 Acryl F *TOTALS NONE GENITAL SYSTEM HEMATOPOIETIC SYSTEM INTEGUMENTARY SYSTEM Mammary Gland M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscop'ically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 35 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED WATER Species/Strain: MICEJB6C3F1 /NCTR ACRYLAMIDE CAS Number: 79-06-1 Pathologist: OLSON, G. DAY ON TEST I 1 O 0 11 11 11 11 I 1 0 0 0 O O 0 0 0 0 0 1 O 0 1 O 0 Date Report Reqsted: 06/2912006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12104 /07112104 lab:NCTR ­ ­- ························1·········· ............................................................................................................................ I 0 0 0 0 0 0 0 0 0 0 0 0 0 10 0 0 3 1 I2 3 3 3 3 4 4 1 4 2 4 3 4 4 + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 erair. Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 C57BLJ6N XC3H/HEN MTV-NCTR MICE FEMALE o o o o o o o o ANIMAL ID 3.52 Acryl f Skin 1 * TOTALS MUSCULOSKELETAL SYSTEM NERVOUS SYSTEM • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically · .. Lesion present .. Insufficient tissue Page 36 TOMS No. 20313- 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Time Report Reqsted: 08:1 5:54 First Dose M/F: 07/12/04 / 07/12/04 Lab: NCTR 1 11 1 0 0 110 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 3 2 3 3 3 4 Lung + + + Nose + + Trachea + Eye Harderian Gland DAY ON TEST Date Report Reqsted: 06/29/2006 1 1 1 1 1 0 0 0 0 0 I 00 0 0 0 0 0 0 4 1 4 2 4 3 + + + + + 8 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 + + + + + + + + 8 .................................... ............... ................................................................................................................ C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE 3.52 Acryl F ANIMAL ID o o o o o o o o I I 4 4 *TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM URINARY SYSTEM SYSTEMIC LESIONS Multiple Organ • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically Page 37 TOMS No. 20313 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Date Report Reqsted: 06129/2006 ACRYLAM IDE Time Report Reqsted: 08:15:54 First Dose MJF: 07/12/04 / 07/12/04 Test Type: 90-DAY Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST '1 10 1 ANIMALID 3.52 Acryl F 1 0 0 0 0 0 0 0 3 3 3 3 1 2 3 4 1 1 1 11 11 0 10 0 0 0 0 0 0 4 14 1 2 4 3 4 4 ......................... ·········· o0 o0 o0 o0 o0 0o o0 o0 o o o o o o o o ........................ , ......... C57BU6N XC3HJHEN MTV-NCTR MICE FEMALE 1 Lab: NCTR *TOTALS *** END OF REPORT **'* • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. nssue examined microscopically .. Lesion present .. Insufficient tissue Page 38 TOMS No. 20314 • 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90.DAY GlYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-0Q..8 First Dose MIF: 07/26/04 / 07126104 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR C Number: C20314 Lock Date: Not Entered. Cage Range: ALL Date iiaii;e: ALL Reasons For Removal: ALL Removal Date Range: ALL Treatment Groups: Include ALL TOMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIOAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07126104 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR o - ~ 1 o l oo o o -o DAY ON TEST 9 9 9 9 9 9 9 9 9 9 9 9 9 9 I 99 .................................. .........·r·........ ·r...................................................................................................... C57BU6N XC3H/HEN MTV-NCTR MICE MALE I 0 0 0 0 0 0 0 0 0 0 0 0 0 0 00 4 4 4 1 4 4 4 4 4 2 3 4 1 2 4 1 3 Esophagus + + + + + + + + 8 Gallbladder + + + + + + + + 8 Intestine Large, Cecum + + + + + + + + 8 Intestine Large, Colon + + + + + + + + 8 Intestine Large, Rectum ++++M+++ 7 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 Liver + + + + + + + + 8 Pancreas + + + + + + + + 8 Salivary Glands + + + + + + + + 8 Stomach, Forestomach + + + + + + + + 8 AN IMAL ID Control Water M 11 1 2 1 212 2 • TOTALS ALIMENTARY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I _ Insufficient tissue Page 2 TOMS No. 2031 4 - 02 Test Type: 90-DAY P04: NEOPLASMS BY INDIVIDUAL ANIMAL GLYCIDAMIDE CAS Number: 5694-00-8 Route: DOSED WATER Species/Strain: MICEJB6C3F1/NCTR Pathologist: OLSON, G. DAy ON TEST I 0 9 9 First Dose M/F: 07/26/04 / 07126104 Lab: NCTR I I I II I I 9 9. 9 9 9 9 9 ............................... 0 9 0 9 0 9 0 ,,- 0 9 9 0 9 0 9 ··············· .................................................................................................................. 0 0 0 0 0 0 0 0 4 4 4 4 Control Water M 1 1 1 2 1 3 1 4 Stomach, Glandular + + + + Tn~ + + + Blood Ves5el + + Heart + Adrenal Cortex M C57BU6N XC3H/HEN MTV-NCTR MICE MAL E Date Report Reqst ed: 06/29/2006 Time Report Reqsted: 08:34:07 0 0 0 00 4 4 4 4 2 1 2 2 2 3 2 4 + + + + 8 + + + + + 8 + + + + + + 8 + + + + + + + 8 + + + + + + + 7 Adrenal Medulla M + + + + + + + 7 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland + + + + + + + + 8 +++++M++ 7 ANIMAL ID tJ e 0 0 0 *TOTALS CARDIOVASCULAR. SYSTEM ENDOCRINE SYSTEM Pituital)' Gland ............................................................................................................................................................................................................. Thyroid Gland + + + + + + + 8 + GENERAL BODY SYSTEM • + x I M .. Missing tissue A .. Autotysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present .. Insufficient lissue Page 3 TOMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED WATER GLYCIDAMIDE CAS Number: 5694-00-8 First Dose MJF: 07/26/04 / 07/26104 Species/Strain: MICEJB6C3F1/NCTR Pathologist: OLSON, G. lab: NCTR 09 l l0 010 o Io 90 9 9 9 9 9 0 0 0 0 0 0 0 0 4 4 4 4 1 1 1 2 1 3 1 4 Epididymis + + + Preputial Gland + + Prostate + Seminal Vesicle Testes Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 o 9 9 9 9 9 9 .......... ................. ..................................... ........................................ DAY ON TEST 9 , 9 9 I 0 0 0 0 0 0 4 4 4 4 1 2 2 2 3 2 4 + + + + + 8 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular + + + + + + + + 8 Lymph Node, Mesent.eric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + + + + 8 C57BLJ6N XC3H/HEN MTV-NCTR MICE MALE Control Water M ANIMAL ID I2 0 0 *TOTALS NONE GENITAL SYSTEM HEMATOPOIETIC SYSTEM • + x I M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. lesion present .. Insufficient tissue Page4 TDMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIDE Dat e Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07126104 Species/St rain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. Lab : NCTR DAY ON TEST C57BLJ6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID Control Water M 0 9 0 9 0 g 0 9 9 9 9 0 0 4 1 0 0 4 0 0 4 0 1 1 1 2 3 0 9 g 0 9 9 0 9 9 0 9 9 0 0 0 0 4 1 4 2 4 2 4 2 4 2 4 1 2 3 4 9 o o' o o o * TOTALS INTEGUMENTARY SYSTEM Mammary Gland M M M M M M M M Skin + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 0 . MUSCULOSKELETALSYSTEM NERVOUS SYSTEM M .. Missing tissue A .. Aulolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tlssue examined microscopically x .. Lesion present 1 .. Insufficient tissue Page 5 TOMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: &C-DAY GLYCIDAMIDE Route: DOSED WATER CAS Number: 5694-00-8 . Pathologist: OLSON, G. SpeciesfStrain: MICEJ86C3F1/NCTR II o9 o9 o9 9 9 90 1 09 1 90 9 9 9 4 4 4 4 1 1 1 2 1 3 1 4 + + + Lung + + Nose + Trachea DAY ON TEST 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose MIF: 07/26/04 / 07/26/04 Lab: NCTA 09 1·09 9 9 ............................... .............. ····1···········································································································.. C578U6N XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 o0 0o o0 MICE MALE 4 4 4 4 212 2 1 2 3 2 4 + + + + + 8 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 Eye + + + .+ + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 Control Water M ANIMAL ID Spinal Cord, Thoracic *TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM URINARY SYSTEM SYSTEMIC LESIONS • .. Total animals with tissue examined microscopically; Total animals with tumor M .. Missing tissue A .. Avtolysis precludes evaluation BLANK .. Not examined microscopically + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 6 TOMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90­DAY GLYCIDAMIDE Route: DOSED WATER CAS Number: 5694­00­8 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID Control Water M Multiple Organ 0 9 9 o· 9 0 9 9 0 0 4 1 3 OI O 0 0 4 4 1 2 4 1 0 0 4 2 2 0 0 4 0 0 4 2 2. + + + + 0 9 90 1 90 9 9 9 0 9 9 0 0 4 0 0 4 1 2 + I~ + 0 9 + Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose M/F: 07/26/04 / 07126104 lab: NCTR 9 9 *TOTALS 3 14 8 + M .. Missing tissue • .. Total animals with tissue examined microscop.ically; Total animals with tumor + .. Tissue examined microscopically A .. Autolysis precludes evaluation BLANK .. Not examined microscopically x .. Lesion present I .. Insufficient tissue Page 7 TOMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL GI­YCIDAMIDE Test Type: 90­DAY Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694­00­8 First Dose M/F: 07/26/04 / 07/26/04 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR DAY ON TEST C57BU6N XC3H/HEN ~· ·· MICE MALE 0.14 Glycid M ANIMAL ID I I I I I o9 o9 o9 o9 o9 o9 o9 1 o9 g · · ·~ g - ~· 0 3 7 1 9 ~ 9 ·~ 0 3 7 2 9 19 · . ·~ 0 3 7 3 0 3 7 4 1 ·~ 9 ·~ 0 3 0 3 1 2 818 9 - ·~ 0 3 8 3 · · ·· 0 3 8 4 ·· ~T O TA LS AUMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM Preputial Gland 1 + HEMATOPOIETIC SYSTEM Spleen 1 + INTEGUMENTARY SYSTEM NONE M .. Missing tissue A .. Aulolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present l .. Insufficient tissue Pages TOMS No. 20314 - 02 Test Type: 90-DAY P04: NEOPLASMS BY INDIVIDUAL ANIMAL GLYCIDAMIDE CAS Number: 5694-00-8 Route: DOSED WATER Species/Strain: MICE/86C3Fl/NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN ~; MICE MALE · · · · · ·~ ANIMAL ID 0.14 Glycid M 0 9 0 9 9 9 -~· a 0 9 9 9 · ~· 9 0 9 9 ·~ ·~ a a 9 9 9 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 First Dose MJF: 07/26/04 / 07/26/04 Lab: NCTR 0 9 9 ·~ T ~· ·· o o o o a o o a 3 3 7 1 7 2 3 7 3 3 3 3 13 3 7 4 8 1 8 2 8 4 8 3 *TOTALS MUSCULOSKELETAL SYSTEM NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE SYSTEMIC LESIONS Multinle Organ • + x I + 2 + M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present .. Insufficient tissue Page9 TDMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694·00-8 First Dose MIF: 07/26/04 / 07/26/04 Species/Strain: MICEJB6C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR DAY ON TEST 0 9 9 I 0 I 0 9 9 s l g , I I I 0 9 g 0 0 g 9 9 I 0 9 19 g I 0 9 g .............................. ................... ........................................................................................................... C57BU6N XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 o0 o0 o0 MICE MALE ANIMAL ID 0.35 G?ycid M I 4 4 4 4 4 4 4 4 5 5 5 5 6 6 6 6 12341234 *TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM Preputial Gland 1 + HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM Skin 1 + M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 10 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20314 - 02 GLYCIDAMIDE CAS Number: 5694­00­8 Test Type: 90-0AY Route: DOSED WATER Species/St rain: MICE/86C3F1 /NCTR · Pathologi st: OLSON, G. 09 1 09 DAY ON TEST 9 C57BU6N XC3H/HEN MTV-NCTR MICE MALE First Dose M/F: 07/26/04 / 07/26/04 Lab: NCTR 90 1 90 1 09 1 90 1 90 1· 90 9 9 9 9 9 9 ................................f................................ o o o o o o o o l ANIMAL ID 0.35 Glycid M 9 Date Report Reqst ed: 06/29/2006 Time Report Reqsted: 08:34:07 1 0 0 0 0 4 4 4 0 4 0 4 0 4 0 4 5 5 5 5 6 16 6 6 1 2 3 4 1 2 4 3 4 + + * TOTALS MUSCULOSKEL ETALSYSTEM NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE SYSTEMIC LESIONS Multiple Organ 2 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 11 TOMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL GLYCIDAMIOE Test Type: 90-DAY Date Report Reqsted: 06/29/2006 Time Report Reqst.e d: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07126104 Species/S1rain: MICEJ86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR DAY ON TEST 0 9 9 0 9 9 9 9 0 0 9 9 0 9 9 0 9 9 0 9 0 9 9 9 ........ ........................ ....... ....................... .................... ...................................................................................... ...... C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 1.41 Glycid M o0 o0 o0 o0 o0 o0 o0 o0 2 5 1 I 2 2 2 2 2 2 2 5 5 5 6 6 6 2 3 4 1 2 I 6 3 + + + + + + + *TOTALS 4 ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE ~ GENITAL SYSTEM Testes + 8 HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM NONE MUSCULOSKELETALSYSTEM M .. Missing tissue A •. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 12 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TUMS No. 20314 - 02 Test Type: 90-DAY G~ YCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Roule: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07126104 Species/Strain: MICEJB6C3F1/NCTR Pathologist: OLSON, G. lab: NCTR DAY ON TEST 010101010 9 9 9 9 0 9 010 9 9 9 , 9 9 9 9 2 5 1 2 5 2 2 5 3 2 5 4 + + + + 9 9 9 9 2 6 1 2 6 2 2 6 3 2 6 4 + + + + ..................................................................................................................................................................... C57BLJ6N XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 0o o0 o0 MICE MALE ANIMAL ID 1.41 Glycid M l *TOTALS NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE SYSTEMIC LESIONS Multiple Organ • + x I 8 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present .. Insufficient tissue Page 13 TDMS No. 20314 - 02 P04: NEOPLASMS Test Type: 90-DAY BY INDIVIDUAL ANIMAL GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted : 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose MIF: 07126104 107126104 Species/Strain: MICEIB6C3F1 /NCTR Pathologist: OLSON, G. lab: NCTR 0 DAY ON TEST 9 9 0 9 9 0 9 0 0 0 0 9 9 9 0 9 9 9 9 9 9 9 9 ···································1·························· ·································································································· 0 0 I 00 0 0 0 0 3 3 3 3 3 1 3 2 3 3 3 4 Esophagus + + + Gallbladder + + Intestine Large, Cecum + Intestine Large, Coton I 0 0 0 0 0 0 0 0 3 3 3 3 4 1 4 2 4 3 4 4 + + + + + 8 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 Intestine Large, Rectum + + + + + + + + 8 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 Liver + + + + + + + + 8 Pancreas + + + + + + + + 8 Salivary Glands + + + + + + + + 8 Stomach, Forestomach + + + + + + + + 8 C57BU 6N XC3H/HEN MTV-NCT R MICE MALE ANIMAL ID 3.52 Glycid M I *TOTALS ALIMENTARY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 14 TOMS No. 20314 - 02 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY GLYCIDAMIDE Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 P.o:Jte: i:­'.JSEI) WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07/26/04 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. Lab: NCTA DAV ON TEST I l II o9 o9 o9 o9 o9 o9 o9 o9 9 9 9 9 9 9 9 , 9 3 3 1 3 3 2 3 3 3 3 ·3 3 4 4 1 3 4 2 3 4 3 3 4 4 Stomach, Glandular + + + + + + + + 8 Tongue + + + + + + + + 8 Blood Vessel + + + + + + + + 8 Heart + + + + + + + + 8 Adrenal Cortex + + + + + + + + 8 Adrenal Medulla + + + + + + + + 8 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland . ++ + M+M++ 6 Pituitary Gland + + + + + + + + 8 Thyroid Gland + + + + + + + + 8 ............................................. ····r ····r···· .................................................................................................. C57BLJ6N XC3HJHEN MTV-NCTR o0 o0 o0 o0 o0 o0 o0 o0 MICE MALE ANIMAL ID 3.52 Glycid M I * TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM GENERAL BODY SYSTEM M .. Missing tissue A .. Aulolysis p recludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 15 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20314 - 02 Test Type: 90-0AY GLYCIDAMIDE Route: DOSED WATER Time Report Reqsted: 08:34:07 CAS Number. 5694-00-8 Species/Strain: MICE/B6C3F1 /NCTR First Dose M/F: 07/26/04 / 07126104 Lab: NCTR . Pathologist: OLSON, G. 0 DAY ON TEST 9 9 0 9 9 0 9 9 0 0 9 9 9 9 0 9 9 0 9 9 Dale Report Reqsted: 06/2912006 0 9 ­ 9 ................................................ ........ .... . ..................................... C57BU6N XC3H/HEN MTV-NCTR MICE MALE o0 o0 o0 o0 o0 o0 o0 o0 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 1 2 3 4 1 2 3 ] 4 Epididymis + + + + + + + + 8 Preputial Gland + + + + + + + + 8 Prostate + + + + + + + + 8 Seminal Vesicle + + + + + + + + 8 Testes + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular + + + + + + + + 8 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + + + + 8 ANIMAL ID 3.52 Glycid M 3 *TOTALS NONE GENITAL SYSTEM HEMATOPOIETIC SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 16 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20314 - 02 Test Type: 90-0AY GLYCIDAMIDE Date Report Reqstecl: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07126104 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. lab: NCTR DAY ON TEST o 9 9 0 10 10 9 9 9 9 9 9 olo o o· 0 0 3 3 0 0 3 3 0 0 3 3 0 0 3 3 1 2 3 4 0 0 3 4 1 9 19 9 9 9 9 9 9 0 0 3 4 0 0 3 4 0 0 3 4 2 3 4 .............................. ..... ........................................................................................................ C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 3.52 Glycid M *TOTALS INTEGUMENTARY SYSTEM Mammary Gland M M M M M M M M Skin + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 0 MUSCULOSKELETALSYSTEM NERVOUS SYSTEM ~ M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 17 TOMS No. 20313- 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTA Pathologist: OLSON, G. DAy ON TEST I Date Report Reqsted: 07/31 /2006 Time Report Reqsted: 12:29:56 First Dose MJF: 08/02104 I 08102104 Lab : NCTR I6 11111111 0 0 0 0 0 0 0 0 6 6 6 6 6 6 6 3 I4 ................................... ··········1··············· ·································································································· C578U6N XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 o0 o0 o0 6 6 s 6 6 6 e 6 MICE MALE Control Feed M ANIMAL ID 1 1 I 1 1 1 12 2 3 4 1 2 2 2 1 2 * TOTALS ALIMENTARY SYSTEM Esophagus + + + + + + + + 8 Gallbladder + + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 + + + Intestine Large, Cecum .......................................................................................................................................................................................................... Intestine Large, Colon ................................................................................................................... .......................................................................................... Intestine Large, Rectum + + + + 8 + ··································································································································································································· Intestine Small, Duodenum + Intestine Small, Ileum + Intestine Small, Jejunum + + + + + + + 8 + ············································································································-·······--·····--······················--·············--·············---········ + + + + + + 8 + ··················-··········-···············-···············································································..································································· + + + + + + 8 + ·········································································--················-····························································---··-······································ + + Liver + Pancreas + + + Salivary Glands + + + + + + + 8 + ••••••••••••o­••• •••••• •· •••••••••••••• • ••••••·••••• • •• ••••••••••• • •••••• •­• •••••••••••••••••• • •••••••••••••••••••••••••••·••o­•••••• ••··•••••••oooo• • •• • ••••••••• •• • •••••••••••••••• • ••••••••• ' Stomach, Forestomach ..... + ·-~ + + + + + + + + + + + 8 + + + + + + 8 8 ............................................................................................................................................................................. ........ ············· ... ·············..........................·······­·····............................................. ··························................................. M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. n ssue examined microscopically x .. Lesion present I .. Insufficient tissue Page 2 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED FEED ACRYLAMIDE CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. I116 6 6 6 6 1 1 2 1 3 Stomach, Glandular + + Tongue + + Blood Vessel Heart DAy ON T EST 1 1 0 0 1 1 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02104 / 08/02104 Lab: NCTR 111 11 6 6 6 I 60 6 6 6 6 6 1 4 2 1 2 3 4 + + + + + + 8 + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 0 0 6 6 0 0 0 ............................................. .... ··········1·································································································· C57BL16N XC3H/HEN MTV-NCTR o0 o0 o0 o0 o0 o0 o0 o0 MICE MALE Control Feed M ANIMAL ID 1 I 2 212 * TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM Adrenal Cortex ··············----···········-·················································································································-··················································· Adrenal Medulla + + + + + + + 8 + ••••••tooooooooo••••Ooooo·oo•o•o • ••o••••• •• ••Ooo•ooo••••••o•oo•ooooo••••• ••• • • • •o••••• • ooo•o•••Oooooooooo•••••••••••H••••••••••••••••••••••••••••ooooooooo•ooo•o•••••oooo••o•o••••••ooooooooooo•••e Islets, Pancreatic + + + + + + + 8 + ········-············································· Parathyroid Gland + + M M + + + M 5 ·······················································--·········································································································································· Pituitary Gland + + + + + + + + + + + + + 8 + .................................................................................................................................................................................................... Thyroid Gland + + 8 GENERAL BODY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. T issue examined microscopically x .. Lesion present I .. Insufficient tissue Page 3 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313- 04 Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1 /NCTR Pathologist: OLSON, G. 16 1 1 0 16 1 0 6 0 6 0 0 6 0 0 6 0 0 6 1 1 1 2 Epididymis + Preputial Gland DAVON TEST 6 0 01 110 I6 6 6 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 I 08/02/04 Lab: NCTR 1 0 6 I 010 0 0 o0 o0 o0 ~4 , ~ 1 6 1 3 + + + + + + + 8 + + + + + + + + 8 Prostate + + + M + + + M 6 Seminal Vesicle + + + + + + + + 8 Testes + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular + + + + + + + + 8 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + + + + 8 C578U6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID Control Feed M 6 6 2 2 1 32 1 24 * TOTALS NONE GENITAL SYSTEM HEMATOPOIETIC SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .... Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page4 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 04 Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Pathologist: OLSON, G. Species/Strain: MICE/B6C3F1 /NCTR 1 11 11 DAy ON TEST ••••• • •• •••• ••• •• • 0 6 l •• ••••• •• C578 Li6N xC3H/HEN MTV-NCTR MICE MALE 00 0 6 1 00 1 , , , ,., 0 6 0 6 0 6 I I 0 6 •••••• 1•• 1 •• •••• •• 00 • • 00 0 6 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 Lab: NCTR I 06 •• ••••• r •• •;• 00 •••• 00 • ••••••• • •oo•• • • •• oo• •oo•••""''"••••••••••oo••oo•••••••••••"""""" """"• ••• oo•••• oo o o o o o o I 0 6 1 1 0 6 1 2 0 6 1 3 0 6 1 4 0 6 2 1 0 6 2 2 0 0 6 6 2 12 3 4 Mammary Gland M M M M M M M M 0 Sk1r1 + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Control Feed M ANIMAL ID *TOTALS INTEGUMENTARY SYSTEM MUSCULOSKELETALSYSTEM NERVOUS SYSTEM • .. Total animals with tissue examined microscopically; Total animals with tumor M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Pages P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 04 Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1 INCTR Pathologist: OLSON, G. I1 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08102104 lab: NCTR 01 01 1 01 1 01 1 01 6 6 6 6 6 6 6 s 6 6 s s 6 1 1 1 2 1 3 1 4 2 1 2 2 2 3 2 4 + + + + + + + + 8 Lung + + + + + + + + 8 Nose + + + + + + + + 8 Trachea + + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 DAY ON TEST C57BU6N XC3H/HEN ~; I~ . .g..i. .i.··g···i···gli.. . . .. ... . .. .... . . .... ·-g MICE MALE ANIMAL ID Control Feed M Spinal Cord, Thoracic 1 0 1 10 6 6 *TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM Eye • Harderian Gland URINARY SYSTEM SYSTEMIC LESIONS • .. Total animals with tissue examined microscopically; Total animals with tumor M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page6 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY AGRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST I 1 I~ 1 1 0 0 1 0 0 6 6 6 6 ·············································· 1 0 0 6 I 6 0 First Dose M/F: 08/02/04108/02/04 Lab: NCTR 0 10 0 0 0 6 ..................................................................................................................... 0 0 0 0 0 0 0 0 6 6 6 6 6 1 1 62 1 62 6 2 2 3 1 4 2 Control Feed M 1 1 1 2 3 4 Multiple Organ + + + + + + + + ANIMAL ID Time Report Reqsted: 12:29:56 ,1'1' 0 0 C57BU6N XC3H/HEN MTV-NCTR MICE MALE Date Report Reqsted: 07/31/2006 0 *TOTALS 8 • .. Total animals with tissue examined microscopically; Total animals with tumor M .. Missing tissue A .. Aulolysis precludes evaluation BLANK .. Not examined microscopically + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 7 TDMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79­06­1 Specis/~lr ain: MICEJ86C3F1/NCTR Pathologist: OLSON, G . DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR . MICE MALE ANIMAL ID 18.5 PPM Acryl M , 0 0 1 6 9 11 0 1 0 6 6 0 0 0 0 6 5 0 6 5 0 6 5 1 2 3 01 6 I0 o o 0 6 110 6 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose MIF: 08/02/04 / 08/02/04 lab: NCTR 1 l 1 0 10 6 6 0 10 10 66 616 6 6 516 4 1 2 3 4 6 *TOTALS ALIMENTARY SYSTEM Esophagus M 0 Gallbladder M 0 Intestine Large, Cecum M 0 Intestine Large, Colon M 0 Intestine Large, Rectum M 0 Intestine Small, Duodenum M 0 Intestine Small, Ileum M 0 Intestine Small, Jejunum M 0 Liver A 0 Pancreas M 0 Salivary Glands M 0 Stomach, Forestomach M 0 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 8 TOMS No. 20313 • 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED FEED ACRYLAMIDE CAS Number: 79-0&1 Pathologist: OLSON, G. Species/Strain: MICE/BSC3F1 /NCTR DAY ON TEST I 1 0 011 1 O 1 0 1 O Date Report Reqsted: 07/31 /2006 Time Report Reqsted: 12:29:5S First Dose M/F: 08/02104 / 08/02104 Lab:NCTR 11111 0 O o s 9 s s s s is s ····································1..··· ....................................................,.............................................. C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 18.5 PPM Acryl M o o o o o o o o 0 0 0 0 0 0 0 0 s s 6 5 1 5 3 5 .2 s s s s 5 6 s Is 4 1 2 3 6 6 4 *TOTALS Stomach, Glandular M 0 Tongue M 0 Blood Vessel M 0 Heart M 0 Adrenal Cortex M 0 Adrenal Medulla M 0 Islets, Pancreatic M 0 Parathyroid Gland M 0 Pituitary Gland M 0 Thyroid Gland M 0 CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM GENERAL BODY SYSTEM M .. Missing tissue A .. Aulolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. lnsuflicienl tissue Page9 TOMS No. 20313- 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-0AY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Pathologist: OLSON, G. Species/Strain: MICE/B6C3F1 /NCTR DAY ON TEST C57BU6N XC3H/HEN ~· · MICE MALE ANIMAL ID 18.5 PPM Acryl M 1 0 Io 1 01 1 01 1 01 1 01 1 01 1 0 6 9 6 6 6 6 r ~· 0 6 5 1 -~· 0 6 5 2 0 6 5 3 0 6 5 4 6 6 · ·~ 0 6 6 1 0 6 6 2 I 1 ·~ · · ·· ·· Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 ·· ·· First Dose M/F: 08/02/04 / 08/02/04 Lab:NCTA ·· ·· ·· ·· ·· 0 0 6 16 6 6 3 4 • TOTALS NONE GENITAL SYSTEM Epididymis M 0 Preputial Gland M 0 Prostate M 0 Seminal Vesicle M 0 Testes A 0 Bone Marrow M 0 Lymph Node, Mandibular M 0 Lymph Node, Mesenteric M 0 Spleen M 0 Thymus M 0 HEMATOPOIETIC SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 10 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90­DAY ACAYLAMIDE Route: DOSED FEED CAS Number: 79­06­1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST 1 0 6 C57BU6N XC3H/HEN MTV­NCTR MICE MALE ANIMAL ID 18.5 PPM Acryl M 0 1 9 1 11 0 0 6 1 6 6 01 6 0 lo 010 0 0 0 0 6 5 6 5 6 5 0 0 6 6 11 2 ' 3 4 1 0 6 5 Date Report Reqsted: 07131/2006 Time Report Reqsted: 12:29:56 Rrst Dose M/F: 08/02104 I 08102104 Lab: NCTR 110 110 6 6 0 0 6 6 0 0 6 6 0 0 6 6 2 3 4 *TOTALS INTEGUMENTARY SYSTEM Mammary Gland M 0 Skin M 0 Bone, Femur M 0 Skeletal Muscle A 0 Brain, Brain Stem M 0 Brain, Cerebellum M 0 Brain, Cerebrum M 0 Paripl A 0 Spinal Cord, Cervical M 0 Spinal Cord, Lumbar M 0 MUSCULOSKELETALSYSTEM NERVOUS SYSTEM : ~ral Neive • .. Total animals with tissue examined microscopically; Total animals with tumor M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically + .. nssue examined microscopically x .. Lesion present I .. Insufficient tissue Page 11 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACAYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE MALE AN IMAL ID 18.5 PPM Acryl M , Io 0 1 6 9 01 6 0 0 0 0 6 5 1 110 10 110 6 6 6 0 0 0 0 0 6 6 5 5 3 6 5 6 6 1 0 0 6 6 2 2 4 0 1 1 0 6 0 6 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/ 02/04 / 08/02104 Lab:NCTR I 616 6 6 o o 0 0 3 * TOTALS 4 ....... Spinal Cord, Thoracic M 0 Lung A 0 Nose M 0 Trachea M 0 Eye M 0 Harderian Gland M 0 Kidney A 0 Urinary Bladder M 0 RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM URINARY SYSTEM SYSTEMIC LESIONS M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. lnsutticient tissue Page 12 TOMS No. 2031 3 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1 /NCTR Pathologist: OLSON, G. DAY ON TEST C57BL/6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 18.5 PPM Acryl M I~ 0 0 0 6 5 1 I 1 1 1 0 0 1 0 0 1 0 0 9 6 6 6 6 6 6 0 0 0 6 0 0 6 0 0 0 6 6 0 6 6 0 0 6 6 1 2 3 4 0 0 6 I 0 6 5 515 2 3 4 1 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08102104 Lab: NCTR 1 6 * TOTALS Multiple Organ 0 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue exc-mined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. lesion present I .. Insufficient tissue Page 13 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 • 04 Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST C57BLJ6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 74 PPM AcryL M 1 110 110 110 111 0 0 10 10 11 0 6 6 6 j 6 6 6 6 6 0 0 0 0 0 0 0 0 0 0 5 7 2 5 7 3 5 7 4 0 5 8 010 0 0 5 7 1 0 5 8 1 2 5 8 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08102/04 Lab: NCTR 5 8 314 *TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM · NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM NONE HEMATOPOIETIC SYSTEM 1 + Spleen INTEGUMENTARY SYSTEM NONE MUSCULOSKELETALSYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 14 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test T ype: 90­DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79­06­1 Pathologi st: OLSON, G. Species/Strain : MICE/86C3F1/NCTR DAY ON TEST C578U6N XC3H/HEN MTV­NCTR MICE MALE ANIMAL ID 7 4 PPM Acryl M I 1 1 0 10 6 6 Date Report Reqsted : 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 I 08102104 Lab: NCTA I 1 1 11 1 11 0 010 0 6 6 6 6 6 6 1 o Io I 010 00 0 0 0 0 0 5 5 5 5 5 7 7 7 7 8 1 12 3 4 2 0 1 0 0 0 0 5 5 5 8 8 8 3 4 0 0 * TOTALS NONE NERVOUS SYSTEM Brain, Cerebrum + 1 + 2 RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE SYSTEMIC LESIONS Multiple Organ • + x I + M .. Missing tissue A ., Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor .. Tissue examined microscopically .. Lesion present . .. Insufficient tissue Page 15 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Date Report Reqsted: 07/31/2006 ACRYLAMIDE Time Report Reqsted: 12:29:56 CAS Number: 79-06-1 Route: DOSED FEED Species/Strain: MICE/B6C3F1/NCTR DAY ON TEST C57BU6N XC3H/HEN ~ · MICE MALE 185 PPM Acryl M First Dose M/F: 08/02/04 / 08102104 Pathologist: OLSON, G. ANIMAL ID 0 1 1 01 1 01 1 1 2 10 0 6 6 6 6 11 · 1 ~· I. · ·~ lab: NCTR 01 1 01 1 01 6 6 6 ·~ · ·· ~ ·· 0 0 0 0 0 0 3 1 3 2 3 3 3 14 4 1 4 2 0 0 4 3 4 4 · · · ·· · · · ·· · ·, ·· ·· · ·· · ·· · ·· · ·· ·· ·· · s s s s s s s s I • * TOTALS ALIMENTARY SYSTEM Esophagus + 1 Gallbladder + 1 Intestine Large, Cecum + 1 Intestine Large, Colon + 1 Intestine Large, Rectum + 1 Intestine Small, Duodenum + 1 Intestine Small, Ileum + Intestine Small, Jejunum + 1 Liver + 1 Pancreas + 1 Salivary Glands + Stomach, Forestomach + 1 • .. Total animals with tissue examined microscopically; Total animals with tumor M .. Missing tissue A .. Autolysls precludes evaluation BLANK .. Not examined microscopically + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 16 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED FEED AGAYLAMIDE Species/Strain: CAS Number: 79-06-1 MICE/86C3F1 /NCTA Pathologist: OLSON, G. DAY ON TEST I 02 1 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 Fi rst Dose M/F: 08/02/04 I 08/02/04 Lab: NCTR 1O 111111 O O O 111111 0 O o 6 6 6 6 6 6 16 .... ................................................ ··········i ···· ...................................................................................................... . C57BU6N XC3H/HEN MTV-NCTR MICE MALE o0 o0 o0 o0 o0 o0 o0 o0 s s s s s s s s 3 3 3 3 4 4 185 PPM_Acryl M 1 2 3 4 1 4 14 2 3 Stomach, Glandular + Tongue + 1 Blood Vessel + 1 Heart + 1 Adrenal Cortex + 1 Adrenal Medulla + 1 Islets, Pancreatic + 1 Parathyroid Gland M 0 Pituitary Gland + 1 Thyroid Gland + 1 ANIMAL ID I * TOTALS 4 CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM GENERAL BODY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 17 TOMS No. 20313 -04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICEJB6C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 185 PPM Acryl M 0 1 1 2 1 0 6 0 6 1 0 6 0 0 0 0 0 0 0 5 5 5 3 2 3 13 3 4 0 5 3 1 1 1 0 6 0 6 6 6 0 0 5 4 0 0 5 4 0 0 5 4 0 0 1 I 2 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 Flrst Dose M/F: 08/02/04 / 08/02104 Lab: NCTR 1 1 0 10 3 5 4 * TOTALS 4 NONE GENITAL SYSTEM 1 Epididymis + Preputial Gland + Prostate + 1 Seminal Vesicle + 1 Testes + + + + + + + + 8 HEMATOPOIETIC SYSTEM Bone Marrow + Lymph Node, Mandibular + 1 Lymph Node, Mesenteric + 1 Spleen + 1 Thymus + M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Nol examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 18 TOMS No. 20313 - 04 TestType: 90-0AY P04: NEOPLASMS BY INDIVIDUAL ANIMAL ACRYLAMIDE Route: DOSED FEED CAS Number: 79·06-1 Species/Strain: MICEIB6C3F1 /NCTR Pathologist: OLSON, G. 2 1 1 0 6 11 0 3 1 3 2 3 3 0 1 0 6 1 Date Report Reqsted: 07/31 /2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08102/04 / 08102/04 Lab: NCTR 11 1 I 1 I o o o o 6 6 6 6 6 .............................................. .................................................................................................................. C57BU6N XC3H/HEN MTV·NCTR o0 o0 o0 o0 o0 o0 o0 0o MICE MALE s s s sIs s s s DAYONTEST 185 PPM Acryl M ANIMAL ID , 314 4 4 1 2 4 4 3 4 *TOTALS INTEGUMENTARY SYSTEM Mammary Gland M 0 Skin + 1 Bone, Femur + 1 Skeletal Muscle + MUSCULOSKELETALSYSTEM + + + + + + 8 + NERVOUS SYSTEM Brain, Brain Stem + Brain, Cerebellum + Brain, Cerebrum + Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 1 • .. Total animals with tissue examined microscopically; Total animals with tumor M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 19 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-0AY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST 110 0 2 1 6 01 01 110 1 0 6 6 6 6 ANIMAL ID 185 PPM Acryl M Spinal Cord, Thoracic 1 0 6 6 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 Lab: NCTR ....... ; .... ·­·.... , ....•.• ... ......................................... •·........................ ............................... .............. · C578U6N XC3H/HEN MTV-NCTR MICE MALE 1 0 Date Report Reqsted: 07/31/2006 o·o ~ 0 0 0 0 0 0 0 0 0 0 5 3 1 5 3 2 5 3 3 5 3 4 5 4 1 5 4 2 5 + + + + + + + 0 0 10 0 5 4 14 3 4 * TOTALS ···························-·····································································-··················································· 8 + RESPIRATORY SYSTEM Lung + 1 Nose + 1 Trachea + 1 Eye + 1 Harderian Gland + 1 Kidney + 1 Urinary Bladder + 1 SPECIAL SENSES SYSTEM URINARY SYSTEM SYSTEMIC LESIONS M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 20 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-0AY ACRYLAMIDE Route: DOSED FEED Species/Strain: MICE/86C3F1/NCTR CAS Number: 79-06-1 Pathologist: OLSON, G. Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 Lab: NCTR I DAY ON TEST C578U6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 185 PPM Acryl M M• J lt i ~!e Organ 0 2 1 1 0 6 00 1 00 5 5 I~ + 3 2 + 1 0 6 1 0 01 6 6 6 6 6 0 0 0 5 0 0 0 5 4 2 0 0 0 0 5 3 3 0 3 5 4 4 1 110 11 0 1 0 5 4 0 5 4 3 4 * TOTALS ······································-·····························································································-··················································· + + + + + 8 + M.. Missing tissue • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically Page 21 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TDMS No. 20313 • 04 Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Pathologist: OLSON, G. Species/Strain: MICE/86C3F1 /NCTR DAY ON TEST C57BL/6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 370 PPM Acryl M 110 I 10 6 6 1 0 6 0 0 0 0 0 0 0 0 4 4 .4 9 9 9 1 2 + + 01 6 I 01 6 o 6 2 1 0 1 0 6 6 0 0 0 0 5 5 0 + 0 0 4 0 5 0 5 9 0 0 3 4 1 2 0 3 + + + A + Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02104 / 08/02104 lab: NCTR *TOTALS 4 ALIMENTARY SYSTEM Esophagus Gallbladder 7 ---····················--·········································································································································································· + + + + + A + 7 + ······················································································­··­­­­­­­­­­­­­················································································ + Intestine Large, Cecum + + + + + Intestine Large, Colon + + + + + + + 7 Intestine Large, Rectum ······································ + + + M + ........................................ + 7 + 7 Intestine Small, Duodenum A + 7 ································································································································································ + + + + + + + A A + ......................................................................................................................................................................................................... Intestine Small, Ileum + + + + + A + + 7 Intestine Small, Jejunum + + + + + A + + 7 Liver + + + + + A + + 7 Pancreas + + + + + A + + 7 Salivary Glands + + + + + A + + 7 Stomach, Forestomach + + + + + A + 7 + M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscop'cally x .. Lesion present I .. Insufficient tissue Page 22 TDMS No. 20313- 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Pathologist: OLSON, G. Species/Strain: MICE/B6C3F1 /NCTA DAY ON TEST 1 0 6 0 6 6 , 6 0 0 4 9 3 + 370 PPM Acryl M 1 0 0 4 9 2 Stomach, Glandular + + C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 0 0 4 9 First Dose M/F: 08/02/04 / 08/02/04 lab: NCTR 1 10 110 1 0110 111 6 0 0 1 Date Report Reqsted: 07/31 /2006 Time Report Reqsted: 12:29:56 6 2 6 6 0 0 5 0 0 0 9 10 4 1 0 0 5 0 2 3 0 4 + A + + 0 0 0 10 4 5 + 5 *TOTALS 7 ··································--··-···················································································································································· + + + + + + + + 8 Blood Vessel + + + + + A + + 7 Heart + + + + + + + + 8 Adrenal Cortex + + + + + A + + 7 Adrenal Medulla + + + + + A + + 7 Islets, Pancreatic + + + + + A + + 7 Parathyroid Gland + + + + M A + + 6 Pituitary Gland + + + + + A + + 7 Thyroid Gland + + + + + A + + 7 Tongue CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM GENERAL BODY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 23 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS Ne. 20313 - 04 Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06·1 Pathologist: OLSON, G. Species/Strain: MICE/86C3F1 /NCTR DAY ON TEST 1 0 11 I 1 1 0 6 1 6 2 6 0 0 0 0 5 0 1 6 0 10 6 0 10 6 0 0 4 9 1 010 0 0 0 0 Epididymis 6 0 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 Lab: NCTR 01 6 213 4 9 4 5 0 0 0 5 0 1 2 3 0 4 + + + + + M + + 7 Preputial Gland + + + + + A + + 7 Prostate +M+++M++ 6 Seminal Vesicle +++++M++ 7 Testes + ++++ M++ 7 Bone Marrow + + + + + A + + 7 Lymph Node, Mandibular + + + + + A + + 7 Lymph Node, Mesenteric + + + + +. A + + 7 Spleen + + + + + A + + 7 Thymus +++ + +M++ 7 C57BU6N XC3H/HEN MTV·NCTR MICE MALE AN IMAL ID 370 PPM Acryl M 4 9 4 9 0 0 5 *TOTALS NONE GENITAL SYSTEM HEMATOPOIETIC SYSTEM ~ M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 24 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 04 Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Time Report Reqsted: 12:29:56 First Dose M/F: 08/02104 / 08/02104 Pathologist: OLSON, G. DAY ON TEST C57BL/6N XC3H/HEN ~· · 1 11 1 10 1 0 0 1 01 1 0 0 6 1 10 1 0 6 2 6 · · · 1 ·~ MICE MALE 370 PPM Acryl M Date Report Reqsted: 07131 /2006 ANIMAL ID 6 -~ 6 · · ·~ 6 6 · ~ · ·~ 6 1 · ·~ 0 ·· ·· 0 0 0 0 0 4 4 4 4 s s s s 19 1 9 2 9 3 9 4 0 1 I 0 2 0 0 0 3 0 4 I Lab: NCTR · · · · · ·· · ·· · ·· · ·· · · · · ·· *TOTALS INTEGUMENTARY SYSTEM Mammary Gland M M M M M M M M Skin + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + A + + 7 Brain, Brain Stem + + + + + A + + 7 Brain, Cerebellum + + + + + A + + 7 Brain, Cerebrum + + + + + A + + 7 Peripheral Nerve + + + + + A + + 7 Spinal Cord, Cervical + + + + + A + + 7 Spinal Cord, Lumbar + + + + + A + + 7 0 MUSCULOSKELETALSYSTEM NERVOUS SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 25 TOMS No. 20313- 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Roule: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTA Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose MIF: 08/02104 / 08/02/04 Lab: NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN ~; 1 0 6 ·~ 1 I, , 1 0 0 0 0 6 6 16 6 · 1 ·~ 1 0 6 I0 1 6 · ~ · ·~ 0 0 0 4 4 9 19 4 9 4 9 1 2 3 + + Lung + Nose Trachea ·· · · · · ·· ·· · · ·· · 0 0 0 0 4 0 1 0 2 0 3 I4 + + + A + + 7 + + + + A + + 7 + + + + + A + + 7 + + + + + A + + 7 + + + + + A + + 7 + + + + + A + + 7 Kidney + + + + + A + 7 Urinary Bladder +++++M++ 7 ~ICE 0 · ·~ 0 6 2 MALE 370 PPM Acryl M ANIMAL ID Spinal Cord, Thoracic s s s s 0 *TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM Eye • Harderian Gland URINARY SYSTEM + SYSTEMIC LESIONS M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 26 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Roule: DOSED FEED ACAYLAMIDE CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTA First Dose M/F: 08/02/04 / 08/02/04 Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 370 PPM Acryl M Multiple Organ 1 1 0 0 10 11 0 0 11 6 6 6 6 6 0 0 4 9 0 0 0 0 I~ l~ + 4 4 9 9 2 3 4 0 0 5 0 1 + + + + 0 6 2 1 0 0 6 6 0 0 5 0 o Io s0 s0 2 3 + + 0 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 Lab: NCTR 1 1 0 I . 4 * TOTALS 8 + *** END OF MALE DATA *** M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined mlcroscopicalty • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present l .. Insufficient tissue Page 27 TOMS No. 20313 · 04 Test Type: 90-DAY P04: NEOPLASMS BY INDIVIDUAL ANIMAL Route: DOSED FEED ,I' I' 1 DAY ON TEST JO 0 7 7 ~; First Dose M/F: 08/02/04 I 08/02104 Pathologist: OLSON, G. Species/Strain: MICE/86C3F1/NCTR C57BU6N XC3H/HEN Date Report Reqst ed: 07/31/2006 Time Report Reqsted: 12:29:56 ACRYLAMIDE CAS Number: 79-06-1 ; ~· ·~ ·~ 0 MICE FEMALE ANIMAL ID Control Feed F 0 7 6 3 1 1 0 7 0 7 · l ·~ 0 6 3 2 0 - ~· 0 6 6 3 3 · ~- 0 6 4 1 3 4 1 0 7 1 1 0 7 0 7 ~ ~ Lab: NCTR ... .... ............................................................................................... 0 6 4 2 0 6 4 3 0 6 4 4 * TOTALS ALIMENTARY SYSTEM ......................................................................................................................... ................................................................................. Esophagus + + + + + + + 8 + ......................................................................................................................................................................................................... Gallbladder Intestine Large, Cecum 8 + + ········­­­­­·····································­­··················­························••to••••·······································­·······································­­··············· 8 + + + + + + + + + + + + + + ........................................................................................................................................................................................................... Intestine Large, Colon + + + + + + + + + + + + + + + + + + + + + + + + + + 8 + ..................................................................................................................................................................................................... Intestine Large, Rectum + + + + 8 + + + + 8 + + + + + + + + + ........................................................................................................................................................................................................ Intestine Small, Duodenum ............................... Intestine Small, Ileum · ~ .....,..........................................................................·····............ -~ ................................................................... 8 .......................................................................................................................................................................................................... Intestine Small, Jejunum + 8 ...................................................................................................................................................................................................... Liver + + + 8 ··················································-················································································································································· Pancreas + + + + + + + + · · ·- Salivary Glands ·· · 8 + + + + + + + + + 8 + + + + + 8 + · · · ·~- · · · · · · · · · · · · ..................................................................................................................................................................................................... Stomach, Forestomach + M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 28 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 04 Test Type: 90-DAY ACAYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN 0 , 1 11 0 0 , · 1 0 0 1 0 0 0 7 7 7 7 7 , 7 7 .........~ .. ·~ MTV-NC~ MICE FEMALE ·~ · ·~ 7 ·~ · · · ~· Date Report Reqsted: 07/31 /2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 Lab: NCTA 1 11 · r -~· · ·~ ............................................................................................... 0 0 0 0 0 0 0 0 6 3 6 6 6 6 4 6 16 4 4 6 4 2 3 4 1 3 3 3 2 3 14 Stomach, Glandular + + + + + + + + 8 Tongue + + + + + + + + 8 Blood Vessel + + + + + + + + 8 Heart + + + + + + + + 8 Adrenal Cortex + + + + + + + + 8 Adrenal Medulla ++++M+++ 7 Islets, Pancreatic + + + 8 Parathyroid Gland M ++ M+M++ 5 Pituitary Gland + + + + + + + + 8 Thyroid Gland M + + + + + + + 7 ANIMAL ID Control Feed F · 1 I *TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM + + + + + GENERAL BODY SYSTEM • .. Total animals with tissue examined microscopically; Total animals with tumor M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 29 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. I 1 I1 1 , , 1 I1 I 1 Date Report Reqsted: 07/3112006 Time Report Reqsted: 12:29:56 First Dose MJF: 08/02104 / 08/02104 Lab: NCTR 1 o o 0 0 o a 0 0 7 ' 7 7 7 7 7 7 7 ................................................................................................................................................................. DAY ON TEST l I 0 0 0 0 0 0 0 0 0 0 0 0 6 3 1 6 3 2 6 3 3 6 3 4 4 41414 6 Clitoral Gland + + + Ovary + + Uterus + Bone Marrow C57BL/6N XC3H/HEN MTV-NCTR MICE FEMALE 0 0 00 6 6 6 1 2 3 4 + + + + + 8 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 Lymph Node, Mandibular + + + + + + + + 8 Lymph Node, Mesenteric + + + + + + + + 8 ~plen + + + + + + + + 8 Thymus + + + + + + + + 8 + + + + + + + + 8 Control Feed F ANIMAL ID • TOTALS NONE AL SYSTEM GEt-~!T HEMATOPOIETIC SYSTEM INTEGUMENTARY SYSTEM Mammary Gland M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 30 TOMS No. 20313 - 04 Test Type: 90-DAY Route: DOSED FEED Species/Strain: MICE/B6C3Fl /NCTA P04: NEOPLASMS BY INDIVIDUAL ANIMAL Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 Lab: NCTR ACAYLAMIDE CAS Number: 79·06-1 Pathologist: OLSON, G. DAY ON TEST 1 0 7 1 1 0 7 0 7 ·~ 0 0 0 6 0 6 3 1 3 2 + Bone, Femur Skeletal Muscle C57BU6N XC3HlHEN MTV-NCTR MICE FEMALE ANIMAL ID Control Feed F Skin 1 0 7 ·~ 1 1 0 0 7 7 -~ · r 1 0 1 0 7 7 ~· · · 1 · ·~ · ·· · · · · · · · · · · · · · · · · · · · · · · · · 0 0 0 0 0 0 6 3 6 3 6 4 6 4 6 16 4 4 3 4 1 2 3 4 + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 + + + + + + + + + + + + + + *TOTALS MUSCULOSKELETALSYSTEM NERVOUS SYSTEM Brain, Brain Stem ........................................................................................................................................................................................................ Brain, Cerebellum + 8 ................................................................................................................................................................................................... • Brain, Cerebrum Peripheral Nerve 8 + ............................................................................................................................................................................. + + Spinal Cord, Cervical 8 + + + + + + ................................................................................................................................................................................................... 8 + + + + + + + + Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 ....................................................................................................................................................................................... M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Nol examined microscopically •.. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 31 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 04 Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Pathologist: OLSON, G. Species/S1rain: MICE/86C3F1/NCTR I 1 0 7 1 1 0 0 10 0 0 0 0 6 0 6 0 6 3 1 3 2 3 3 3 4 Lung + + + Nose + + Trachea + Eye + Harderian Gland I1 Date Report Reqsted: 07/31 /2006 Time Report Reqst ed: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 Lab: NCTR 7 7 7 1 0 7 0 0 0 0 0 0 6 0 6 4 0 6 4 0 6 0 6 4 4 1 2 3 4 + + + + + 8 + + + + + + 8 + + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 + + + + + + + + 8 DAY ON TEST 1 0 7 C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID Control Feed F 7 7 1 1 0 10 * TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM URINARY SYSTEM SYSTEMIC LESIONS Multiple O!'gan M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor · x .. Lesion present I .. Insufficient tissue + .. Tissue examined microscopically Page 32 TOMS No. 20313 - 04 Test Type: 90-DAY P04: NEOPLASMS BY INDIVIDUAL ANIMAL ACRYLAMIDE Route: DOSED FEED CAS Number: Species/Strain: MICEJB6C3F1/NCTR 79­06­1 Pathologist: OLSON, G. DAY ON TEST ANIMAL ID 185 PPM Acryl F 07/31/2006 12:29:S6 Fi rst Dose M/F: 08/02/04 / 08/02/04 Lab: NCTR 111111111111111 0 0 0 0 0 0 0 0 7 C57BLJ6N XC3H/HEN MTV-NCTR MICE FEMALE Date Report Reqsted: Time Report Reqsted: 7 7 7 7 7 7 7 o o!o o o o olo 0 0 0 0 0 0 0 0 s s s s s s s s s s i s s s s 6l 6 1 2 3 4 1 2 3 * TOTALS 4 ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM ··································································································································································-······································· Ovary + + + + + + + 8 + HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM NONE MUSCULOSKELETALSYSTEM • .. Total animals with tissue examined microscopically; Total animals with tumor M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 33 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Date Report Reqsted: 07/31/2006 ACRYLAMIDE Route: DOSED FEED Time Report Reqsted: 12:29:56 CAS Number: 79­06­1 Species/Strain: MICE/86C3F1/NCTR First Dose M/F: 08/02/04 I 08102104 Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN ~· ·· ·· 1 11 11 1 0 0 0 ,1 7 ~ · · 1 ·~ 0 0 7 · ~· 7 · ~· Lab: NCTR 1 ! 1 11 11 0 10 0 10 7 7 7 7 .., · ~· · ~ · ,· · ~ · · Ii" ~ 0 0 0 0 0 0 5 6 3 ·· ·· · ·· · · · · · · ·· ·· ·· ·· ·· · · · · · ·· · · · · 0 s s s s s s s s MICE FEMALE 5 3 5 4 6 1 I2 6 185 PPM Aery! F 1 2 Skeletal Muscle + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 ANIMAL ID 5 1 I 6 *TOTALS 4 NERVOUS SYSTEM RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE SYSTEMIC LESIONS • .. Total animals with tissue examined microscopically; Total animals with tumor M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 34 P04: NEOPLASMS BY INDIVIDUAL ANIMAL IDMS No. 20313 - 04 Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79­06­1 Species/Strain: MICE/B6C3F1 /NCTR C57BL/.6N XC3H/HEN First Dose M/F: 08/02/04 / 08102104 Pathologist: OLSON, G. DAY ON TEST 111 0 10 7 7 ~;·- li ·~ 0 1 1 0 7 ·~ 0 · 0 ·~ 0 7 · -~· 0 · ·~ 0 · ·~ 0 s s s s s s MICE FEMALE ANIMAL ID 185 PPM Aery! F Multiple Organ 515 5 1 2 3 + + + Lab: NCTR 1 11 11 0 0 0 7 7 7 1 0 7 ·~- · ·· 0 0 5 5 5 6 6 616 4 1 2 3 4 + + + + + · ·· Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 · · · · · ......... ........................................... . *TOTALS 8 M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. l esion present I .. Insufficient tissue Page 35 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1 /NCTA Pathologist: OLSON, G. DAVON TEST 1 1 0 7 0 7 1 0 7 1 0 7 0 7 1 0 7 0 0 5 1 1 0 0 5 0 0 5 0 0 5 0 0 5 0 0 5 1 11 12 3 4 1 2 2 1 1 0 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 I 08102104 Lab: NCTR 1 7 0 7 0 0 0 5 2 5 2 3 4 ······························ ................................. .................................................................................................... C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 370 PPM Acryl F 1 2 0 *TOTALS ALIMENTARY SYSTEM ···································································-···························-····································································································· Esophagus + + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 ...................................................................................................................................................................................................... Gallbladder Intestine Large, Cecum ...................................................................................................................................................................................................... Intestine Large, Colon Intestine Large, Rectum 8 + + + + + + + + ........................................................................................................................................................................................................ + + + + + + + + 8 ····································································································································································································· Intestine Small, Duodenum + + + + + + + 8 + . ····­·························································­ ··· ···­........ .. ..................... ... ....4.,............ ........... .... .................................................................. Intestine Small, Ileum + + + + + + + + 8 •• • •­•••••Oooo••••••u••••••••••••••••••••••••••••••••••••••••••OO•O• • ••••••••••••••••••••••••• • • oo••••••••••••••••••••••••••••••••••••••••••••••••.,•••••• • •••••••••••••• • •••••••••••• ••• •••••••••••• Intestine Small, Jejunum + + + + + + + + + + + + + + + + + + + + + + + 8 ..................................................................................................................................................................................................... Liver 8 ..................................................................................................................................................................................................... Pancreas Salivary Glands 8 + ..................................................................................................................................................................................................... 8 + + + + + + + + • ­ • • • • • • • • • • • • . . OOOO•OOo•• • • • •·• ­ • • • • • • • • • • • • • • • • • o •OOOOOOOOOoo ­ •oo • • o • • • o o • • • • • • ­• • • • • • • • • • • • . , • • o o o o o 0 • • • • • • • • • • ­ • • • • • • 0 000 000 0 000 0 000000000000000• 0 0•0 0 000.00oooooooooo•••• • •Oo•OOOOO.OOOOOoooooo•••o Stomach, Forestomach + + + + + + + 8 + M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically · x .. Lesion present I .. Insufficient tissue Page 36 P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20313 - 04 Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST 1 0 7 C57BU6N XC3H/HEN MTV-NCTR MICE FEMALE ANIMAL ID 370 PPi\,1 Acryl F I 01 I 01 7 7 0100 0 0 ' 0 5 11 5 1 2 5 113 1 1 0 7 1 0 1 1 0 0 7 7 7 0 10 0 0 0 0 0 7 0 5 1 4 Date Report Reqsted: 07131 /2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02104 / 08/02104 Lab: NCTR .............··1·................................................................................................. 0 5 5 0 5 2 1 2 2 2 3 I 0 5 I 2 4 * TOTALS Stomach, Glandular + + + + + + + + 8 Tongue + + + + + + + + 8 Blood Vessel ++MM++++ 6 Heart + + + + + + + + 8 + + + + + + + + 8 CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM Adrenal Cortex ··································································································································································································· Adrenal Medulla + + + + + + + 8 + ····················································--····················································-······························-··--··-············-·-··································· Islets, Pancreatic Parathyroid Gland + + + + + + + + + M + + + + + 8 + 7 ............................................................................................................................................................................................. ··································································································································································································· Pituitary Gland + + + + + + + + 8 ······························-···································································································································································· Thyroid Gland + + + + + + + 8 + GENERAL BODY SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 37 TOMS No. 20313- 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST 11O 1 0 7 1 11 11 0 O 0 7 7 7 1 0 7 1 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08102104 Lab: NCTR 1 0 7 0 7 I 00 I 0 0 7 ..................................................r..······· .................................................................................................. I0 0 0 1 1 11 Clitoral Gland + Ovary Uterus I 0 1 314 1 2 12 12 12 1 2 3 4 + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node, Mandibular + + + + + + + + 8 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Thymus + + + + + + + + 8 + + + + + + + + 8 370 PPM Acryl F ANIMAL ID 0 0 0 0 C57BU6N xc . 3H/HEN MTV-NCTR MICE FEMALE 0 0 0 0 s s s s s s s s 2 *TOTALS NONE GENITAL SYSTEM HEMATOPOIETIC SYSTEM INTEGUMENTARY SYSTEM M:.rnmary Gland M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 38 TOMS No. 20313 - 04 Test Type: 90­DAY Route: DOSED FEED Species/Strain: MICEJB6C3F1/NCTR P04: NEOPLASMS BY INDIVIDUAL ANIMAL ACRYLAMIDE CAS Number: 79­06­1 Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN ~; 1 0 0 0 0 11 0 1 0 0 1 0 7 7 7 7 7 7 7 7 ..........~ .. -·~ 0 1 1 1 ·~ 0 1 1 ·~ 0 1 ·~ 0 · ·~ ·· 0 0 0 0 ·· Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02/04 Lab: NCTR ·· · · · ·· · ·· ·· ·· · · · · s s s s s s s s MICE FEMALE ANIMAL ID 1 1 11 11 1 2 3 4 2 1 2 2 2 3 2 4 + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Siem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + M 7 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 370 PPM Acryl F Skin *TOTALS MUSCULOSKELETALSYSTEM NERVOUS SYSTEM M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 39 TOMS No. 20313- 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED FEED CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTA Pathologist: OLSON, G. DAVON TEST 01 Date Report Reqsted: 07/31/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02/04 / 08/02104 Lab: NCTR 110 11 0 I 01 11 0 01 11 0 11 0 7 7 7 7 0 0 0 5 0 0 0 0 5 3 1 4 2 1 5 2 2 0 5 2 3 0 0 5 2 4 + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 Eye + + + + + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + + 8 + + + + + + + 8 7 7 7 17 0 0 5 1 0 0 5 1 0 0 5 1 1 2 Lung + Nose Trachea C57BL/6N XC3H/HEN MTV·NCTR MICE FEMALE . ANIMAL ID 370 PPM Acryl F * TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM URINARY SYSTEM SYSTEMIC LESIONS Multiple Organ + M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 40 TOMS No. 20313 - 04 P04: NEOPLASMS BY INDIVIDUAL ANIMAL Test Type: 90­DAY ACRYLAMIDE Rout e: DOSED FEED CAS Number: 79­06­1 Species/Strain: MICE/86C3F1/NCTR Date Report Reqsted: 07131/2006 Time Report Reqsted: 12:29:56 First Dose M/F: 08/02104 / 08102104 Lab: NCTR Pathologist: OLSON, G. DAY ON TEST 01 1 01 17 7 10 11111 0 0 0 7 7 1 3 4 7 7 2 1 2 2 01 1 01 7 7 ................................... ........... ,......................................................................... o0 o0 o0 o0 o0 o0 o0 o0 C57B U6N XC3H/HEN MTV­NCTR MICE FEMALE 5 5 s 5 5 5 s s I ANIMAL ID 370 PPM Acryl F I1 i 1 1 2 1 I 2 3 2 4 • TOTALS *** END OF REPORT *** M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue Page 41 Pathology Report Appendix IV Non-neoplastic Lesions by Individual Animal (Pathology Report 9) A-4 TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-0AY Route: DOSED WATER ACRYLAMIDE CAS Number: 79-06-1 Species/Strain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. C Number: C20313 Lock Date: Not Entered_ Cage Range: ALL Date Range: ALL Reasons For Removal: ALL Removal Date Range: ALL Treatment Groups: Include ALL Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12104 / 07/12104 Lab: NCTR TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90.DAY Rout e: DOSED WATER ACRYLAMIDE CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST I 0 9 9 I I I I I I I I 09 0 9 9 0 9 9 0 9 9 0 9 9 0 9 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 I 07112104 Lab: NCTR 0 9 9 .... .. . . ... . . .. .r... ····r··........... . ......................................................................................................... C57BU6N XC3HIHEN MTV-NCT:l MICE MALE gggggggg o o o o o o o o I5 5 2 I3 5 4 6 1 1 6 2 6 3 6 4 Esophagus + + + + + + + + 8 Gallbladder +++++M++ 7 Intestine large, Cecum + + + + + + + + 8 Intestine Large, Colon + + + + + + + + 8 Intestine Large, Rectum + + + + + + + + 8 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 Liver Tension Lipidosis + + + + + + + + 8 Pancreas + + + + + + + + 8 Salivary Glands + + + + + + + + 8 Stomach, Forestomach + + + + + + + + 8 Control Water M ANIMAL ID 9 5 * TOTALS ALIMENTARY SYSTEM 1 2.0 2 • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation BLANK .. Not examined microscopically I .. Insufficient tissue + .. Tissue examined microscopically Page 2 1·4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4} Marked IDMS No. 20313 • 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED WATER ACRYLAMIDE CAS Number: 79-06-1 Species/Strain: MICE/86C3F1/NCTR · Pathologist: OLSON, G. Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12104 / 07/12104 Lab:NCTR o l o l o o o o ol o DAY ON TEST 9 19 9 9 9 9 9 9 9 9 19 9 9 9 9 , 9 0 0 0 0 0 0 0 0 0 0 0 0 .......................·1·.................. ................................................................................................................. C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID I 0 0 0 0 o o o o o o o o 5 1 5 2 5 3 5 4 6 1 6 Control Water M 2 6 3 6 4 Stomach, Glandular + + + + + + + + 8 Tongue + + + + + + + + 8 Blood Vessel + + + + + + + + 8 Heart + + + + + + + + 8 Adrenal Cortex Subcapsular, Hyperplasia + + + 1 + 1 + + + 1 + 8 Adrenal Medulla + + + + + + + + 8 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland + + + + + + + + 8 Pituitary Gland M + + + + + + + 7 Thyroid Gland + + + + + + + + 8 *TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM 3 1.0 .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation BLANK .. Not examined microscopically I .. Insufficient tissue Page 3 ~ + .. Tissue examined microscopically 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2} Mild 4) Marked TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACAYLAMIDE CAS Number: 79-06-1 Route: DOSED WATER Species/Strain: MICE/86C3F1/NCTR Pathologist OLSON, G. DAy ON TEST I l 0 10 9 9 9 9 0 9 9 0 9 9 0 9 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/ 12104 / 07/12104 Lab: NCTR 0 10 10 9 9 9 9 9 9 ···································r ·······-············1·····-································································································· C57BU6N XC3H/HEN MTV-NCTR MICE MALE I 0 0 0 0 0 0 0 0 l 00 0 0 0 0 0 0 0 0 6 3 6 4 I 0 0 0 0 0 0 5 1 5 5 2 , 3 5 4 6 1 6 2 Epididymis + + + + + + + + 8 Prepulial Gland Cyst + + + + + + + + 8 ANIMAL ID Control Water M [ • TOTALS GENERAL BODY SYSTEM NONE GENITAL SYSTEM 3 2 3.0 3 ·····-·································································································································--·-········· Prostate + + + + + + + + 8 ···································································-············································································-···························-·-·····-· Seminal Vesicle + + + + + + + 8 + ···············-···-····················································································---····················.. ······················-·······-.. + + + + + + + + 8 Bone Marrow + + + + + + + + 8 Lymph Node. Mandibular ++MM++++ 6 Lymph Node, Mesenteric + + + + + + + + 8 Spleen + + + + + + + + 8 Testes # HEMATOPOIETIC SYSTEM • + x I .. Total animals wi1h tissue examined microscopically; Total animals with lesion and mean severity grade .. Tissue examined microscopically M .. Missing tissue .. Lesion present A .. Autolysis precludes evaluation .. Insufficient tissue BLANK .. Not examined microscopically Page4 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 4) Marked 2) Mild TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type : 90·DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICEJB6C3F1/NCTA Pathologist: OLSON, G. l I I o o 0 Date Report Reqsted: 06/29/2006 Time Repo rt Reqsted: 08:15:54 First Dose M/F: 07112/04 / 07/12/04 Lab: NCTR 09 1 90 o 9 o 9 o 9 9 19 9 9 9 9 9 9 9 9 9 •.•. .•.•.•.••.•.•.•.•..•••.•..•••. ·1·••. •,•..•.•.••....... ., .•.• , •.•.• ......••......•.....••...•. ••...•.• .....•.....••. •. ..•• .....•......•.•.•.•.•...••••.. .•.••... 0 0 0 0 0 0 0 0 C57BU6N XC3H/HEN MTV-NCTR 0 0 0 0 0 0 0 0 MICE MALE 0 0 0 0 0 0 0 0 DAY ON TEST ANIMAL ID Control Water M Thymus 5 1 5 2 5 5 6 6 6 3 4 1 2 3 6 4 + + + + + + + + 8 M+ MM 2 * TOTALS INTEGUMENTARY SYSTEM Mammary Gland MMM+ Skin + + + + + + + + 8 Bone, Femur + + + + + + + + 8 Skeletal Muscle + + + + + + + + 8 Brain, Brain Stem + + + + + + + + 8 Brain, Cerebellum + + + + + + + + 8 Brain, Cerebrum + + + + + + + + 8 Peripheral Nerve + + + + + + + + 8 i~al s with MUSCULOSKELETAL SYSTEM NERVOUS SYSTEM • .. Total animals with tissue examined microscopically; Total an + .. Tissue examined microscopically x .. l esion present I .. Insufficient tissue lesion and mean severity grade M .. Missing tissue A .. Autolysis precludes evaluation BLANK .. Not examined microscopically Pages 1­4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-0AY ACRYLAMIDE CAS Number: 79-06-1 Route: DOSED WATER Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN ~· 0 0 0 0 0 9 9 9 9 9 9 9 9 9 -·~ 9 f ~ T ·~- r ~· · ·~- 0 9 9 0 0 9 9 9 - ·~- Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07112/04 Lab: NCTR 9 · ·~ · · 0 0 0 0 0 0 0 0 5 1 5 2 5 3 5 4 6 1 6 2 6 3 6 4 Spinal Cord, Cervical + + + + + + + + 8 Spinal Cord, Lumbar + + + + + + + + 8 Spinal Cord, Thoracic + + + + + + + + 8 Lung + + + + + + + + 8 Nose + + + + + + + + 8 Trachea + + + + + + + + 8 Eye + + + + + + + + 8 Harderian Gland + + + + + + + + 8 Kidney + + + + + + + + 8 Urinary Bladder + + + + + + + 8 o o o o o o o o MICE MALE ANIMAL ID Control Water M 1 *TOTALS RESPIRATORY SYSTEM SPECIAL SENSES SYSTEM URINARY SYSTEM • + x I + .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade .. Tissue examined microscopically M .. Missing tissue A .. Autolysis precludes evaluation .. Lesion present BLANK .. Not examined microscopically .. Insufficient tissue Page6 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 4) Marked 2) Mild TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL ACRYLAMIOE CAS Number: 79~06-1 Test Type: 90-0AY Route: DOSED WATER Species/S1rain: MICE/B6C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST C57BU6N XC3H/HEN ~;: ANIMAL ID • + x I Lab: NCTR ihli···g i" ..i .g···.. . ...................... 9 9 9 9 9 9 9 ·~ o o o o o o o o MICE MALE Control Water M Time Report Reqsted: 08:1 5:54 First Dose MJF:­07/12104 / 07/12104 ol ol o ol o o ol o 9 9 9 9 19 9 9 I 9 9 .. ..• Date Report Reqsted: 06/29/2006 sjs 1 2 5 I3 5 4 6 1 6 2 6 3 6 4 *TOTALS .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade .. Tissue examined microscopically M .. Missing tissue .. Lesion present A .. Autolysis precludes evaluation .. Insufficient tissue BLANK .. Not examined microscopically Page 7 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 4) Marked 2) Mild TOMS No. 20313-02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL ACRYLAMIDE Test Type: 90-0AY Route: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICEJB6C3F1/NCTR · Pathologist: OLSON, G. DAY ON TEST 0 0 0 9 9 9 9 9 9 0 9 9 0 9 9 0 9 9 0 ­o 9 9 9 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07/12104 / 07/12104 Lab: NCTR .......................... '"' ...... , ........................................................................................................................................... C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 0.35 Acryl M 0 0 0 0 0 0 0 0 1111 0 0 0 0 0 0 0 0 1111 3 3 3 3 4 4 4 4 1 2 3 4 1 2 3 4 + + *TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NON E GENERAL BODY SYSTEM NONE- GENITAL SYSTEM Preputial Gland Cyst 2 3 1 3.0 HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM NONE • __ Total animals Y.~th tissue examined microscopically; Total animals with lesion and mean severity grade M __ Missing tissue x __ Lesion present A __ Autolysis precludes evaluation I .. Insufficient tissue BLANK __Not examined microscopically + .. Tissue examined microscopically Page a 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20313- 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Date Report Reqsted: 06/29/2006 Test Type: 90-DAY Route: DOSED WATER ACRYLAMIDE Time Report Reqsted: 08:15:54 First Dose M/F: 07/12/04 / 07/ 12104 Lab: NCTR CAS Number: 79­0S­1 Pathologist: OLSON, G. Species/Strain: MICE/B6C3F1/NCTA DAY ON TEST . IIIII I o9 o9 o9 o9 o9 o· 9 1o 9 o 9 9 9 9 9 9 9 0 0 0 0 0 t 4 1 1 1 1 4 2 4 3 4 4 I 9 9 .............................·1·... ..................·r...................................................................................................... C578U6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 0.35 Acryl M I o0 l 1 3 1 o o o o o 0o o0 1 1 1 2 3 3 3 4 I3 *TOTALS MUSCULOSKELETALSYSTEM NONE NERVOUS SYSTEM NONE RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade + .. Tissue examined microscopically M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically Page9 1·4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20313 ­ 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE CAS Number: 79­06­1 Route: DOSED WATER Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAY ON TEST 0 09 1 9 0 19 9 . . . . . . ...... . . . . . . . . . . . . . ... . . . · C57Bl16N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 0.70 Acryl M ~ 9 9 90 1 90 1 90 1 09 9 9 9 9 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose MJF: 07/12/04 / 07/12/04 Lab: NCTR 90 9 ... . " l """ ... •.• . .... ~ ••• • · -· • • •• , . . . . . . . . . . . . . ..... . . . .... . . . . . . . . . . . . . . . .. 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 111111 1 1 7 7 7 7 8 8 8 8 1 2 3 4 1234 * TOTALS ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE . ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM NONE HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM NONE MUSCULOSKELETALSYSTEM • .. Total an imals with tissue examined microscopically; Total animals w ith lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis precludes evaluation BLANK .. Not examined microscopically I .. Insufficient tissue + .. Tissue examined microscopically Page 10 1­4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY Route: DOSED WATER ACRYLAMIDE CAS Number: 79·06­1 Pathologist: OLSON, G. Specie.s/Strain: MICEJ86C3F11NCTR DAy ON TEST I~ ~ Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 Rrst Dose MJF: 07/12104 / 07/12/04 Lab: NCTR ~ ~ ~ ~ ~ ~ 0 10 10 10 10 10 10 10 .................. ,,. ...... ................. ......... ...... ........ . C57BLJ6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 0.70 Acryl M Skeletal Muscle 0 0 oI o 0 0 1 7 1 0 0 1 7 2 0 0 7 3 81 1 11 8 4 2 8 3 + + + + + + + + 8 + + + + + + + + 8 1 1 7 0 10 0 0 0 1 0 1 8 4 *TOTALS NERVOUS SYSTEM Peripheral Nerve RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade + .. Tissue examined microscopically M... Missing tissue x .. Lesion present A .. Autolysis precludes evaluation BLANK .. Not examined microscopically I .. Insufficient tissue Page 11 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked TOMS No. 20313 ­ 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90·DAY Route: DOSED WATER CAS Number: 79-06-1 ACRYLAMIDE Species/Strain: MICE/86C3F1/NCTR Pathologist: OLSON, G. DAy ON TEST C578U6N XC3H/HEN ~ · MICE MALE Io9 l o9 9 1.41 Acryl M o9 0 ! 0 10 10 10 9 9 9 9 9 9 9 9 9 9 9 ·iri· · g····ili . i. ·g · ·~ 0 ANIMAL ID 9 9 1 I 1 0 0 0 9 2 9 3 9 4 + + + I Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 First Dose M/F: 07112104 / 07112104 Lab: NCTR · · · ··· · ·· · 1 1 1 1 0 1 0 2 0 3 0 4 *TOTALS + + + + 8 I ALIMENTARY SYSTEM NONE CARDIOVASCULAR SYSTEM NONE ENDOCRINE SYSTEM NONE GENERAL BODY SYSTEM NONE GENITAL SYSTEM Testes + HEMATOPOIETIC SYSTEM NONE INTEGUMENTARY SYSTEM N0N:: MUSCULOSKELETAL SYSTEM • .. Total animals with tissue examined microscopically; Total animals with lesion and mean severity grade M .. Missing tissue x .. Lesion present A .. Autolysis preciudes evaluation I .. Insufficient tissue BLANK .. Not examined microscopically + .. Tissue examined microscopically Page 12 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Mar1<ed TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type : 90-DAY ACRYLAMIDE Rou te: DOSED WATER CAS Number: 79-06-1 Species/Strain: MICE.186C3F1/NCTR · Pathologist: OLSON, G. 90 1 90 1 90 1 09 0 0 9 9 0 0 9 9 9 9 0 0 0 0 0 1 ,I 9 2 0 0 0 9 3 9 4 0 1 + + + + Peripheral Nerve + + + Spinal Cord, Cervical + + Spinal Cord, Lumbar + Spinal Cord, Thoracic + DAY ON TEST 9 C57BU6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 1.41 Acryl M S k e!~• I Muscle 9 I 0 9 0 1 0 Re.port Reqsted: 06/29/2006 First Dose MJF: 07112/04 / 07112104 Lab: NCTR °I° 9 9 9 0 0 1 0 0 0 .............. , .......... .... . o0 o0 Dat e Time Report Reqsted: 08:15:54 9 1 2 0 3 14 + + + + 8 + + + + + 8 + + + + + + 8 + + + + + + + 8 + + + + + + + 8 * TOTALS NERVOUS SYSTEM RESPIRATORY SYSTEM NONE SPECIAL SENSES SYSTEM NONE URINARY SYSTEM NONE • + x I .. Total animals with tissue examined microscopically; Total animals wilh lesion and mean severity grade .. Tissue examined microscopically M .. Missing tissue .. Lesion presenl A .. Autolysis precludes evaluation .. Insufficient tissue BLANK .. Nol examined microscopically Page 13 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 4) Marked 2) Mild TOMS No. 20313 - 02 P09: NON-NEOPLASTIC LESIONS BY INDIVIDUAL ANIMAL Test Type: 90-DAY ACRYLAMIDE Route: DOSED WATER CAS Number: 79-06-1 Species/Strain : MICE/86C3F1/NCTR First Dose M/F: 07/12104 / 07/12/04 Pathologist : OLSO N, G. 0 DAY ON TEST 0 919 9 9 0 9 0 9 9 9 0 9 9 0 9 9 90 9 0 9 9 0 0 0 0 0 0 Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:15:54 Lab: NCTR ....................................................................................................................................................................... 0 I 0 0 0 0 0 o0 o0 0 0 0 0 0 1 1 1 2 2 3 1 4 Esophagus + + + Gallbladder + + Intestine Large, Cecum + Intestine Large, Colon C57BLJ6N XC3H/HEN MTV-NCTR MICE MALE 11 2 2 3 0 0 2 4 + + + + + 8 + + + + + M 7 + + + + + + + 8 + + + + + + + + 8 Intestine Large, Rectum + + + + + + + + 8 Intestine Small, Duodenum + + + + + + + + 8 Intestine Small, Ileum + + + + + + + + 8 Intestine Small, Jejunum + + + + + + + + 8 Liver Inflammation, Chronic Active Tension Upidosis + + + + + + + 1 + 8 Pancreas + + + + + + + + 8 Salivary Glands + + + + + + + 8 ANIMAL ID 3.52 Acryl M 1 0 2 . * TOTALS ALIMENTARY SYSTEM 1 1.0 1 2.0 2 + ~ .. fo!a ~ '3nimals with tissue examined microscopically; Total animals with lesion and mean severity grade + .. Tissue examined microscopically M .. Missing tissue x .. Lesion present I .. Insufficient tissue A .. Autolysi s precludes evaluation BLANK .. Not examined microscopically Page 14 1-4 .. Lesion qualified as: 1) Minimal 3) Moderate 2) Mild 4) Marked P04: NEOPLASMS BY INDIVIDUAL ANIMAL TOMS No. 20314 - 02 GLYCIDAMIOE Test Type: 90-0AY Date Report Reqsted: 06/29/2006 Time Report Reqsted: 08:34:07 Route: DOSED WATER CAS Number: 5694-00-8 First Dose M/F: 07/26/04 / 07/26/04 Species/Strai n: MICE/B6C3F1/NCTR Pathologist: OLSON, G. Lab: NCTR 9 9 0l 0 919 9 9 0 0 0 0 0 0 0 0 3 3 3 3 4 4 1 4 4 2 13 4 4 + + + + + + 8 + + + + + + + 8 + + + + + + + + 8 + + + + + + + + 8 Adrenal Cortex + + + + + + + + 8 Adrenal Medulla + + + + + + + + 8 Islets, Pancreatic + + + + + + + + 8 Parathyroid Gland +++M+M++ f. Pituitary Gland + + + + + + + + 8 Thyroid Gland + + + + + + + + 8 09 10 9 9 9 9 90 1 90 9 9 0 0 0 0 0 0 0 0 3 3 1 3 3 2 3 3 3 3 3 Stomach, Glandular + + Tongue + Blood Vessel Heart DAY ON TEST C578U6N XC3H/HEN MTV-NCTR MICE MALE ANIMAL ID 3.52 Glycid M 0 9 0 * TOTALS CARDIOVASCULAR SYSTEM ENDOCRINE SYSTEM GENERAL BODY SYSTEM • .. Total animals with tissue examined microscopically; Total animals with tumor + .. Tissue examined microscopically x .. Lesion present I .. Insufficient tissue M .. Missing tissue A ·- Autolysis precludes evaluat.ion BLANK .. Not examined microscopically Page 15